FvH4_3g12250

Glutathione S-transferase omega-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0005011
Physical Location & Seq
Reverse (-)
7257624 .. 7260456
2833 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g12250.t2

Sequence Viewer

Length: 978 bp
ATGGCTCGGTCTGCACTGGATGAGGTTTCGCAATCTGGTGCATTTGTGAGAAGCGCTTCTGAATTCCGTAACTTCATTTCACGAGACCCGAATTCGCAGTTTCCAGCTGAAGCTGGGAGATATCATCTGTACATATCATATGCTTGCCCGTGGGCGTCAAGGTGCCTTGCATACTTAAATATCAAAGGACTTCAGAAAGCCATCAGCTTTACGTCGGTCAAACCAATATGGGAAAGAACAAAAGAAAGTGATGAGCATATGGGATGGGTTTTTGCTGCTTCGGATAAGGAGCTAGCAGGAGCTGAACCTGACCCCTTGAATGGAGCAAAAAGTGTTCGAGAACTTTATGAGATTGCTAGTACAAAATACACTGGAAAGTACACTGTTCCGGTTCTGTGGGATAAAAAGCTTGGAACAATTGTCAGTAATGAGAGTTCAGAGATAATTCGCATGTTCAATACTGAATTCAACAGTATAGCAGAGAACCCAGCTTTGGACTTGTATCCAACTCACTTGCAATCCCAAATCAATCAGACTAATGAATGGATATACGACAAGATAAACAACGGTGTCTATAAATGTGGGTTTGCCAAGAAGCAGGAGCCTTATGATGAGGCTGTGAAACAGTTGTATGAAGCATTGGACAGATGTGAGGAGATACTTAGCAAGCAACGATACCTTTGTGGAAACACATTGTCTGAAGCAGATATTCGCTTGTTCGTTACTCTCATAAGATTCGATGAGGTTTATGTAGTCCACTTCAAGTGCAACAAGAAACTCCTGCGAGAGTACCCGAATCTGTTTAACTACACCAAAGACATCTTCCAAGTTCCAGGAATGAGCAGCACAGTCCAGATGGATCACATCAAACAACACTACTACGGAAGCCACCCTTCCATCAATCCTTTCGGAATAGTTCCATCCGGCCCCAACATCGACTACTCTTCCCCTCATGACAGATACAAGTTTTCTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GST_C_2 GST_N_2

Protein Analysis

326

Amino Acids

37.24

Weight (kDa)

6.47

Isoelectric Point (pI)

42.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N_2 PF13409 68 - 174 4.7e-10 Glutathione S-transferase, N-terminal domain
GST_C PF00043 216 - 296 2e-09 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 222 - 291 2e-14 Glutathione S-transferase, C-terminal domain
GST_C_3 PF14497 225 - 299 3.6e-07 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 162
AclWI GGATC 1 cut(s) 867
AcsI RAATTY 3 cut(s) 62, 91, 464
AcuI CTGAAG 3 cut(s) 129, 176, 720
AcyI GRCGYC 1 cut(s) 155
AfaI GTAC 4 cut(s) 131, 361, 380, 791
AfeI AGCGCT 1 cut(s) 55
AfiI CCNNNNNNNGG 2 cut(s) 320, 493
AgsI TTSAA 4 cut(s) 319, 457, 469, 763
AjnI CCWGG 1 cut(s) 832
AjuI GAANNNNNNNTTGG 4 cut(s) 476, 508, 806, 838
AluBI AGCT 7 cut(s) 107, 113, 207, 292, 302, 409, 491
AluI AGCT 7 cut(s) 107, 113, 207, 292, 302, 409, 491
Alw26I GTCTC 1 cut(s) 78
AlwI GGATC 1 cut(s) 867
AlwNI CAGNNNCTG 1 cut(s) 302
Aor51HI AGCGCT 1 cut(s) 55
AoxI GGCC 1 cut(s) 925
ApeKI GCWGC 2 cut(s) 275, 843
ApoI RAATTY 3 cut(s) 62, 91, 464
Asp700I GAANNNNTTC 1 cut(s) 55
AspLEI GCGC 1 cut(s) 56
AspS9I GGNCC 1 cut(s) 926
AsuNHI GCTAGC 1 cut(s) 292
BaeI ACNNNNGTAYC 2 cut(s) 667, 700
BanI GGYRCC 1 cut(s) 162
BauI CACGAG 1 cut(s) 81
BbvI GCAGC 2 cut(s) 262, 855
BccI CCATC 5 cut(s) 209, 258, 850, 905, 928
BciT130I CCWGG 1 cut(s) 834
BciVI GTATCC 1 cut(s) 513
BcoDI GTCTC 1 cut(s) 78
BfaI CTAG 2 cut(s) 293, 357
BfoI RGCGCY 1 cut(s) 57
BfuI GTATCC 1 cut(s) 513
BisI GCNGC 2 cut(s) 276, 844
BlsI GCNGC 2 cut(s) 277, 845
Bme1390I CCNGG 1 cut(s) 834
BmgT120I GGNCC 1 cut(s) 926
BmiI GGNNCC 3 cut(s) 164, 603, 928
BmrFI CCNGG 1 cut(s) 834
BmtI GCTAGC 1 cut(s) 296
BsaHI GRCGYC 1 cut(s) 155
BsaI GGTCTC 1 cut(s) 78
BsaJI CCNNGG 1 cut(s) 149
BsaWI WCCGGW 1 cut(s) 388
Bsc4I CCNNNNNNNGG 2 cut(s) 320, 493
Bse1I ACTGG 2 cut(s) 21, 376
BseBI CCWGG 1 cut(s) 834
BseDI CCNNGG 1 cut(s) 149
BseGI GGATG 3 cut(s) 25, 269, 920
BseLI CCNNNNNNNGG 2 cut(s) 320, 493
BseNI ACTGG 2 cut(s) 21, 376
BseRI GAGGAG 1 cut(s) 668
BseXI GCAGC 2 cut(s) 262, 855
BseYI CCCAGC 2 cut(s) 113, 487
BshFI GGCC 1 cut(s) 927
BshNI GGYRCC 1 cut(s) 162
BsiSI CCGG 2 cut(s) 389, 924
BslI CCNNNNNNNGG 2 cut(s) 320, 493
BsmAI GTCTC 1 cut(s) 78
BsnI GGCC 1 cut(s) 927
Bso31I GGTCTC 1 cut(s) 78
Bsp1407I TGTACA 1 cut(s) 129
Bsp143I GATC 1 cut(s) 859
BspANI GGCC 1 cut(s) 927
BspHI TCATGA 1 cut(s) 952
BspLI GGNNCC 3 cut(s) 164, 603, 928
BspOI GCTAGC 1 cut(s) 296
BspPI GGATC 1 cut(s) 867
BspT107I GGYRCC 1 cut(s) 162
BspTNI GGTCTC 1 cut(s) 78
BsrGI TGTACA 1 cut(s) 129
BsrI ACTGG 2 cut(s) 21, 376
BssECI CCNNGG 1 cut(s) 149
BssMI GATC 1 cut(s) 859
BssNI GRCGYC 1 cut(s) 155
BssSI CACGAG 1 cut(s) 81
Bst2BI CACGAG 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 834
Bst4CI ACNGT 5 cut(s) 385, 473, 569, 627, 850
Bst6I CTCTTC 1 cut(s) 949
BstACI GRCGYC 1 cut(s) 155
BstAUI TGTACA 1 cut(s) 129
BstC8I GCNNGC 3 cut(s) 145, 294, 668
BstDEI CTNAG 1 cut(s) 662
BstDSI CCRYGG 1 cut(s) 149
BstF5I GGATG 3 cut(s) 25, 269, 920
BstH2I RGCGCY 1 cut(s) 57
BstHHI GCGC 1 cut(s) 56
BstKTI GATC 1 cut(s) 862
BstMAI GTCTC 1 cut(s) 78
BstMBI GATC 1 cut(s) 859
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 1 cut(s) 834
BstNSI RCATGY 1 cut(s) 454
BstSCI CCNGG 1 cut(s) 832
BstV1I GCAGC 2 cut(s) 262, 855
BsuI GTATCC 1 cut(s) 513
BsuRI GGCC 1 cut(s) 927
BtgI CCRYGG 1 cut(s) 149
BtsCI GGATG 3 cut(s) 25, 269, 920
BtsIMutI CAGTG 3 cut(s) 14, 369, 381
Cac8I GCNNGC 3 cut(s) 145, 294, 668
CaiI CAGNNNCTG 1 cut(s) 302
CciI TCATGA 1 cut(s) 952
CfoI GCGC 1 cut(s) 56
Cfr13I GGNCC 1 cut(s) 926
CseI GACGC 1 cut(s) 144
Csp6I GTAC 4 cut(s) 130, 360, 379, 790
CviAII CATG 2 cut(s) 451, 953
CviQI GTAC 4 cut(s) 130, 360, 379, 790
DdeI CTNAG 1 cut(s) 662
DpnI GATC 1 cut(s) 861
DpnII GATC 1 cut(s) 859
Eam1104I CTCTTC 1 cut(s) 949
EarI CTCTTC 1 cut(s) 949
Eco31I GGTCTC 1 cut(s) 78
Eco32I GATATC 1 cut(s) 122
Eco47III AGCGCT 1 cut(s) 55
Eco57I CTGAAG 3 cut(s) 129, 176, 720
EcoRI GAATTC 3 cut(s) 62, 91, 464
EcoRII CCWGG 1 cut(s) 832
EcoRV GATATC 1 cut(s) 122
FaeI CATG 2 cut(s) 454, 956
FalI AAGNNNNNCTT 2 cut(s) 877, 909
FatI CATG 2 cut(s) 450, 952
FauNDI CATATG 2 cut(s) 139, 258
Fnu4HI GCNGC 2 cut(s) 276, 844
FokI GGATG 3 cut(s) 32, 276, 907
Fsp4HI GCNGC 2 cut(s) 276, 844
FspBI CTAG 2 cut(s) 293, 357
GlaI GCGC 1 cut(s) 55
GluI GCNGC 2 cut(s) 276, 844
GsaI CCCAGC 2 cut(s) 117, 491
HaeII RGCGCY 1 cut(s) 57
HaeIII GGCC 1 cut(s) 927
HapII CCGG 2 cut(s) 389, 924
HgaI GACGC 1 cut(s) 144
HhaI GCGC 1 cut(s) 56
Hin1I GRCGYC 1 cut(s) 155
Hin1II CATG 2 cut(s) 454, 956
Hin6I GCGC 1 cut(s) 54
HinP1I GCGC 1 cut(s) 54
HindIII AAGCTT 1 cut(s) 407
HinfI GANTC 2 cut(s) 735, 796
HpaII CCGG 2 cut(s) 389, 924
Hpy166II GTNNAC 2 cut(s) 381, 757
Hpy188I TCNGA 7 cut(s) 61, 195, 283, 439, 534, 700, 911
Hpy188III TCNNGA 4 cut(s) 81, 338, 853, 953
Hpy8I GTNNAC 2 cut(s) 381, 757
Hpy99I CGWCG 1 cut(s) 217
HpyAV CCTTC 1 cut(s) 903
HpyCH4III ACNGT 5 cut(s) 385, 473, 569, 627, 850
HpyCH4IV ACGT 1 cut(s) 212
HpyCH4V TGCA 6 cut(s) 14, 41, 170, 517, 768, 974
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 1 cut(s) 662
HpySE526I ACGT 1 cut(s) 212
Hsp92I GRCGYC 1 cut(s) 155
Hsp92II CATG 2 cut(s) 454, 956
HspAI GCGC 1 cut(s) 54
Kzo9I GATC 1 cut(s) 859
LmnI GCTCC 4 cut(s) 289, 299, 323, 601
Lsp1109I GCAGC 2 cut(s) 262, 855
MaeI CTAG 2 cut(s) 293, 357
MaeII ACGT 1 cut(s) 212
MaeIII GTNAC 2 cut(s) 68, 721
MalI GATC 1 cut(s) 861
MboI GATC 1 cut(s) 859
MboII GAAGA 2 cut(s) 814, 936
MfeI CAATTG 1 cut(s) 417
MluCI AATT 5 cut(s) 62, 91, 417, 444, 464
MmeI TCCRAC 1 cut(s) 530
MnlI CCTC 5 cut(s) 16, 607, 646, 736, 960
MroXI GAANNNNTTC 1 cut(s) 55
MseI TTAA 2 cut(s) 176, 804
MspA1I CMGCKG 1 cut(s) 107
MspI CCGG 2 cut(s) 389, 924
MspR9I CCNGG 1 cut(s) 834
MunI CAATTG 1 cut(s) 417
MvaI CCWGG 1 cut(s) 834
MwoI GCNNNNNNNGC 1 cut(s) 11
NdeI CATATG 2 cut(s) 139, 258
NdeII GATC 1 cut(s) 859
NheI GCTAGC 1 cut(s) 292
NlaIII CATG 2 cut(s) 454, 956
NlaIV GGNNCC 3 cut(s) 164, 603, 928
NspI RCATGY 1 cut(s) 454
PagI TCATGA 1 cut(s) 952
PdmI GAANNNNTTC 1 cut(s) 55
PfeI GAWTC 2 cut(s) 735, 796
PfoI TCCNGGA 1 cut(s) 832
PkrI GCNGC 2 cut(s) 277, 845
Psp6I CCWGG 1 cut(s) 832
PspFI CCCAGC 2 cut(s) 113, 487
PspGI CCWGG 1 cut(s) 832
PspN4I GGNNCC 3 cut(s) 164, 603, 928
PspPI GGNCC 1 cut(s) 926
PsrI GAACNNNNNNTAC 2 cut(s) 418, 450
PstNI CAGNNNCTG 1 cut(s) 302
PvuII CAGCTG 1 cut(s) 107
RsaI GTAC 4 cut(s) 131, 361, 380, 791
RsaNI GTAC 4 cut(s) 130, 360, 379, 790
SaqAI TTAA 2 cut(s) 176, 804
SatI GCNGC 2 cut(s) 276, 844
Sau3AI GATC 1 cut(s) 859
Sau96I GGNCC 1 cut(s) 926
ScrFI CCNGG 1 cut(s) 834
Sse9I AATT 5 cut(s) 62, 91, 417, 444, 464
SspMI CTAG 2 cut(s) 293, 357
StyD4I CCNGG 1 cut(s) 832
TaaI ACNGT 5 cut(s) 385, 473, 569, 627, 850
TaiI ACGT 1 cut(s) 215
TaqI TCGA 3 cut(s) 337, 738, 936
TaqII GACCGA 1 cut(s) 205
TasI AATT 5 cut(s) 62, 91, 417, 444, 464
TatI WGTACW 3 cut(s) 129, 359, 378
TfiI GAWTC 2 cut(s) 735, 796
Tru1I TTAA 2 cut(s) 176, 804
Tru9I TTAA 2 cut(s) 176, 804
TscAI CASTG 3 cut(s) 21, 376, 388
TseI GCWGC 2 cut(s) 275, 843
TspDTI ATGAA 3 cut(s) 64, 555, 648
TspGWI ACGGA 2 cut(s) 56, 897
TspRI CASTG 3 cut(s) 21, 376, 388
XapI RAATTY 3 cut(s) 62, 91, 464
XceI RCATGY 1 cut(s) 454
XmnI GAANNNNTTC 1 cut(s) 55
XspI CTAG 2 cut(s) 293, 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.