MD05G1252100.v1.1

Glutathione S-transferase omega-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005011
Physical Location & Seq
Forward (+)
38376470 .. 38377873
1404 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1252100.v1.1.491

Sequence Viewer

Length: 927 bp
ATGGCTCAAACTACGGAGATATCACAATCCGGTGCATTTTTGAGAACTGCTTCTGTGTTCCAACCAGGGAGATATCATCTGTATATATCATATGGTTGCCCTTGGGCTTCTAGGTGCCTTGCATACTTGAAAATCAAAGGACTTGAGAAAGCCATCAGCTTTACGTCAGTCAAACCAATGTGGGGAAGAACAAAAGAAAGCGATGAGCATATGGGGTTGCTTTTTCCTGCTTCAGATGCCGAGGTAGCTGGAGCTGAACCTGACCCTTTGAATGGAGCAAAAAGTATTAGAGAACTTTACGAACTTGCAAGCACACAGTATTCCGGAAAGTACACAGTTCCAGTTCTATGGGATCAAAAACTTAAAACAATTGTCAACAACGAGAGTGCAGAGATAATTCGTATGTTTAATACTGAATTCAACGACATGGCTGAAAACGCATCTATGGACTTATATCCTCCTCACTTGCAATCTCAAATTGATCAGACAAATGAATGGATATATGACATGATAAATAACGGTGTCTATAAATGTGGGTTTGCGAGGAAGCAGGAGCCTTATGACGAGGCTGTGAAAGAATTGTTCGAAGCTTTGGATAAATGCGAGGAGATACTTAGCAAGCAACGATACTTGTGTGGGAACACATTGTCTGAAGCAGATATTCGGTTGTTTGTCACTCTTATAAGATTCGACGAGGCTTACGCAGTCAACTTCAAGTGCAACAAGAAACTGCTACGAGAATACCCGAATTTGTTTAACTACACGAAAGATATCTTCCAAGTTCCTGGAGTGAGCAGCTCAGTAAACATGGAGCACATAAGGCGAGGCTATTACGGCCTTCTGGCTGTCAATCCATCCGGAATAGTTCCTATCGGCCCAAATATCGACTACTCTTCTCCTCATGACAGAGACAGGTTTTCTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GST_C_2 GST_N_2

Protein Analysis

309

Amino Acids

35.1

Weight (kDa)

5.52

Isoelectric Point (pI)

33.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N_2 PF13409 33 - 138 3.3e-10 Glutathione S-transferase, N-terminal domain
GST_C PF00043 180 - 260 4.4e-09 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 186 - 255 3e-14 Glutathione S-transferase, C-terminal domain
GST_C_3 PF14497 192 - 265 2.5e-07 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 683
AccB1I GGYRCC 1 cut(s) 114
AccIII TCCGGA 2 cut(s) 323, 857
AclWI GGATC 1 cut(s) 360
AcsI RAATTY 2 cut(s) 416, 748
AcuI CTGAAG 2 cut(s) 216, 672
AfaI GTAC 1 cut(s) 332
AfiI CCNNNNNNNGG 2 cut(s) 182, 272
AgsI TTSAA 4 cut(s) 130, 271, 421, 715
AjnI CCWGG 2 cut(s) 64, 784
AluBI AGCT 5 cut(s) 159, 248, 254, 590, 798
AluI AGCT 5 cut(s) 159, 248, 254, 590, 798
Alw21I GWGCWC 1 cut(s) 816
Alw26I GTCTC 1 cut(s) 903
AlwI GGATC 1 cut(s) 360
Aor13HI TCCGGA 2 cut(s) 323, 857
AoxI GGCC 2 cut(s) 835, 874
ApeKI GCWGC 1 cut(s) 795
ApoI RAATTY 2 cut(s) 416, 748
Asp700I GAANNNNTTC 1 cut(s) 49
AspS9I GGNCC 1 cut(s) 875
AsuII TTCGAA 1 cut(s) 585
BaeI ACNNNNGTAYC 2 cut(s) 619, 652
BanI GGYRCC 1 cut(s) 114
Bbv12I GWGCWC 1 cut(s) 816
BbvI GCAGC 1 cut(s) 807
BccI CCATC 2 cut(s) 161, 862
BceAI ACGGC 1 cut(s) 850
BciT130I CCWGG 2 cut(s) 66, 786
BclI TGATCA 1 cut(s) 481
BcoDI GTCTC 1 cut(s) 903
BfaI CTAG 1 cut(s) 111
BisI GCNGC 1 cut(s) 796
BlsI GCNGC 1 cut(s) 797
Bme1390I CCNGG 2 cut(s) 66, 786
BmgT120I GGNCC 1 cut(s) 875
BmiI GGNNCC 2 cut(s) 116, 555
BmrFI CCNGG 2 cut(s) 66, 786
BmsI GCATC 2 cut(s) 226, 449
BpmI CTGGAG 2 cut(s) 270, 807
Bpu14I TTCGAA 1 cut(s) 585
BpuEI CTTGAG 1 cut(s) 164
BsaJI CCNNGG 3 cut(s) 65, 101, 240
BsaWI WCCGGW 3 cut(s) 29, 323, 857
Bsc4I CCNNNNNNNGG 2 cut(s) 182, 272
Bse1I ACTGG 1 cut(s) 341
BseAI TCCGGA 2 cut(s) 323, 857
BseBI CCWGG 2 cut(s) 66, 786
BseDI CCNNGG 3 cut(s) 65, 101, 240
BseGI GGATG 1 cut(s) 854
BseLI CCNNNNNNNGG 2 cut(s) 182, 272
BseMII CTCAG 1 cut(s) 813
BseNI ACTGG 1 cut(s) 341
BseRI GAGGAG 3 cut(s) 450, 620, 888
BseXI GCAGC 1 cut(s) 807
BsgI GTGCAG 1 cut(s) 408
BshFI GGCC 2 cut(s) 837, 876
BshNI GGYRCC 1 cut(s) 114
BsiHKAI GWGCWC 1 cut(s) 816
BsiSI CCGG 3 cut(s) 30, 324, 858
BslI CCNNNNNNNGG 2 cut(s) 182, 272
BsmAI GTCTC 1 cut(s) 903
BsnI GGCC 2 cut(s) 837, 876
Bsp119I TTCGAA 1 cut(s) 585
Bsp1286I GDGCHC 1 cut(s) 816
Bsp13I TCCGGA 2 cut(s) 323, 857
Bsp143I GATC 2 cut(s) 352, 481
BspANI GGCC 2 cut(s) 837, 876
BspCNI CTCAG 1 cut(s) 812
BspEI TCCGGA 2 cut(s) 323, 857
BspHI TCATGA 1 cut(s) 901
BspLI GGNNCC 2 cut(s) 116, 555
BspPI GGATC 1 cut(s) 360
BspT104I TTCGAA 1 cut(s) 585
BspT107I GGYRCC 1 cut(s) 114
BsrI ACTGG 1 cut(s) 341
BssECI CCNNGG 3 cut(s) 65, 101, 240
BssMI GATC 2 cut(s) 352, 481
BssT1I CCWWGG 1 cut(s) 101
Bst2UI CCWGG 2 cut(s) 66, 786
Bst4CI ACNGT 3 cut(s) 318, 337, 521
Bst6I CTCTTC 1 cut(s) 898
BstBI TTCGAA 1 cut(s) 585
BstC8I GCNNGC 2 cut(s) 310, 620
BstDEI CTNAG 2 cut(s) 614, 799
BstF5I GGATG 1 cut(s) 854
BstKTI GATC 2 cut(s) 355, 484
BstMAI GTCTC 1 cut(s) 903
BstMBI GATC 2 cut(s) 352, 481
BstMWI GCNNNNNNNGC 5 cut(s) 236, 245, 437, 820, 834
BstNI CCWGG 2 cut(s) 66, 786
BstSCI CCNGG 2 cut(s) 64, 784
BstV1I GCAGC 1 cut(s) 807
BstXI CCANNNNNNTGG 2 cut(s) 348, 785
BsuRI GGCC 2 cut(s) 837, 876
BtgZI GCGATG 1 cut(s) 216
BtsCI GGATG 1 cut(s) 854
Cac8I GCNNGC 2 cut(s) 310, 620
CciI TCATGA 1 cut(s) 901
Cfr13I GGNCC 1 cut(s) 875
Csp6I GTAC 1 cut(s) 331
CviAII CATG 4 cut(s) 427, 508, 808, 902
CviQI GTAC 1 cut(s) 331
DdeI CTNAG 2 cut(s) 614, 799
DpnI GATC 2 cut(s) 354, 483
DpnII GATC 2 cut(s) 352, 481
Eam1104I CTCTTC 1 cut(s) 898
EarI CTCTTC 1 cut(s) 898
Eco130I CCWWGG 1 cut(s) 101
Eco32I GATATC 3 cut(s) 21, 74, 772
Eco57I CTGAAG 2 cut(s) 216, 672
EcoRI GAATTC 1 cut(s) 416
EcoRII CCWGG 2 cut(s) 64, 784
EcoRV GATATC 3 cut(s) 21, 74, 772
EcoT14I CCWWGG 1 cut(s) 101
ErhI CCWWGG 1 cut(s) 101
FaeI CATG 4 cut(s) 430, 511, 811, 905
FatI CATG 4 cut(s) 426, 507, 807, 901
FauNDI CATATG 2 cut(s) 91, 210
FbaI TGATCA 1 cut(s) 481
Fnu4HI GCNGC 1 cut(s) 796
FokI GGATG 1 cut(s) 841
Fsp4HI GCNGC 1 cut(s) 796
FspBI CTAG 1 cut(s) 111
GluI GCNGC 1 cut(s) 796
GsuI CTGGAG 2 cut(s) 270, 807
HaeIII GGCC 2 cut(s) 837, 876
HapII CCGG 3 cut(s) 30, 324, 858
Hin1II CATG 4 cut(s) 430, 511, 811, 905
HincII GTYRAC 2 cut(s) 376, 709
HindII GTYRAC 2 cut(s) 376, 709
HindIII AAGCTT 1 cut(s) 588
HinfI GANTC 1 cut(s) 687
HpaII CCGG 3 cut(s) 30, 324, 858
Hpy166II GTNNAC 4 cut(s) 333, 376, 709, 805
Hpy188I TCNGA 3 cut(s) 235, 486, 652
Hpy188III TCNNGA 3 cut(s) 324, 858, 902
Hpy8I GTNNAC 4 cut(s) 333, 376, 709, 805
Hpy99I CGWCG 1 cut(s) 695
HpyAV CCTTC 1 cut(s) 848
HpyCH4III ACNGT 3 cut(s) 318, 337, 521
HpyCH4IV ACGT 1 cut(s) 164
HpyCH4V TGCA 6 cut(s) 35, 122, 308, 389, 469, 720
HpyF10VI GCNNNNNNNGC 5 cut(s) 236, 245, 437, 820, 834
HpyF3I CTNAG 2 cut(s) 614, 799
HpySE526I ACGT 1 cut(s) 164
Hsp92II CATG 4 cut(s) 430, 511, 811, 905
Kpn2I TCCGGA 2 cut(s) 323, 857
Ksp22I TGATCA 1 cut(s) 481
Kzo9I GATC 2 cut(s) 352, 481
LmnI GCTCC 4 cut(s) 251, 275, 553, 811
Lsp1109I GCAGC 1 cut(s) 807
LweI GCATC 2 cut(s) 226, 449
MaeI CTAG 1 cut(s) 111
MaeII ACGT 1 cut(s) 164
MaeIII GTNAC 1 cut(s) 673
MalI GATC 2 cut(s) 354, 483
MboI GATC 2 cut(s) 352, 481
MboII GAAGA 3 cut(s) 198, 766, 885
MfeI CAATTG 1 cut(s) 369
MhlI GDGCHC 1 cut(s) 816
MluCI AATT 6 cut(s) 369, 396, 416, 477, 578, 748
MmeI TCCRAC 1 cut(s) 85
MnlI CCTC 9 cut(s) 235, 468, 471, 537, 559, 598, 688, 818, 909
MroI TCCGGA 2 cut(s) 323, 857
MroXI GAANNNNTTC 1 cut(s) 49
MseI TTAA 3 cut(s) 363, 408, 756
MspI CCGG 3 cut(s) 30, 324, 858
MspR9I CCNGG 2 cut(s) 66, 786
MunI CAATTG 1 cut(s) 369
MvaI CCWGG 2 cut(s) 66, 786
MwoI GCNNNNNNNGC 5 cut(s) 236, 245, 437, 820, 834
NdeI CATATG 2 cut(s) 91, 210
NdeII GATC 2 cut(s) 352, 481
NlaIII CATG 4 cut(s) 430, 511, 811, 905
NlaIV GGNNCC 2 cut(s) 116, 555
NmeAIII GCCGAG 1 cut(s) 265
NmuCI GTSAC 1 cut(s) 673
NspV TTCGAA 1 cut(s) 585
PagI TCATGA 1 cut(s) 901
PdmI GAANNNNTTC 1 cut(s) 49
PfeI GAWTC 1 cut(s) 687
PfoI TCCNGGA 1 cut(s) 784
PkrI GCNGC 1 cut(s) 797
PsiI TTATAA 1 cut(s) 683
Psp6I CCWGG 2 cut(s) 64, 784
PspGI CCWGG 2 cut(s) 64, 784
PspN4I GGNNCC 2 cut(s) 116, 555
PspPI GGNCC 1 cut(s) 875
RsaI GTAC 1 cut(s) 332
RsaNI GTAC 1 cut(s) 331
SaqAI TTAA 3 cut(s) 363, 408, 756
SatI GCNGC 1 cut(s) 796
Sau3AI GATC 2 cut(s) 352, 481
Sau96I GGNCC 1 cut(s) 875
ScrFI CCNGG 2 cut(s) 66, 786
SduI GDGCHC 1 cut(s) 816
SfaNI GCATC 2 cut(s) 226, 449
SfuI TTCGAA 1 cut(s) 585
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
Sse9I AATT 6 cut(s) 369, 396, 416, 477, 578, 748
SspMI CTAG 1 cut(s) 111
StyD4I CCNGG 2 cut(s) 64, 784
StyI CCWWGG 1 cut(s) 101
TaaI ACNGT 3 cut(s) 318, 337, 521
TaiI ACGT 1 cut(s) 167
TaqI TCGA 3 cut(s) 585, 690, 885
TasI AATT 6 cut(s) 369, 396, 416, 477, 578, 748
TatI WGTACW 1 cut(s) 330
TfiI GAWTC 1 cut(s) 687
Tru1I TTAA 3 cut(s) 363, 408, 756
Tru9I TTAA 3 cut(s) 363, 408, 756
TseFI GTSAC 1 cut(s) 673
TseI GCWGC 1 cut(s) 795
Tsp45I GTSAC 1 cut(s) 673
TspDTI ATGAA 1 cut(s) 507
TspGWI ACGGA 1 cut(s) 29
XapI RAATTY 2 cut(s) 416, 748
XmnI GAANNNNTTC 1 cut(s) 49
XspI CTAG 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.