MD10G1233200.v1.1

Glutathione S-transferase omega-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005011
Physical Location & Seq
Forward (+)
32930078 .. 32932469
2392 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1233200.v1.1.491

Sequence Viewer

Length: 957 bp
ATGACCTCACTATCAGGTGCATTTGTGAGAACTGCTTCTGTATTTCGTAACTTCATTTCGCGGGACCCGAATTCGCAATTTCCAGCAGAACCTGGAAGATATCATCTGTATATATCATATGCCTGCCCTTGGGCTTCCAGGTGCCTTGCATACTTGAACATAAAAGGACTCGATAAAGCCATCAGCTTTACGTCAGTCAAACCGATATGGGAAAGGACAAAAGAAAGTGATGAACATATGGGGTGGGTTTTTCCTGCTTTCGAAACAGAGCTAGCGGGAGCTGAACCTGACCCTTTGAATGGAGCAAAAAGCATTAGAGAACTTTACGAGCTTGCAAGCACACAGTATACCGGAAAGTACACAGTTCCAGTTCTATGGGATAAAAAACTCAAAACAATTGTCAGTAATGAGAGCGGGGAGATTATTCGCATGTTCAATACTGAATTCAACGATATAGCTGAAAACGCGTCTTTGGACTTATATCCTCCTCACTTGCAATCCCAAATTGATCAGACAAATGAATGGATATATGACAAGATAAACAACGGTGTCTATAAATGCGGGTTTGCGAGGAAGCAAGAGCCTTATGATGAGGCTGTGAAACAATTGTACGAAGCTTTGGATAAATGTGAGGAGATACTTAGCAAGCAACGATACCTGTGCGGGAACACATTGTCTGAAGCAGATATTCGGTTGTTTGTCACTCTTATAAGATTCGACGAGGTTTACGTAGTCCACTTCAAGTGCAACAAGAAAGTACTACGAGAATACCTGAATTTGTTCAACTACACCAAAGACATTTTCCAGGTTCCGGGGATGAGCAGCACAGTCAACATGGATCACATAAAGCGACACTACTACGGAAGCCATCCGTCTATCAACCCATTCGGAATAATTCCTAGCGGGCCAGATATCGACTACTCGTCTCCTCACGACAGAAACAGGTTTTCGACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GST_C_2 GST_N_2

Protein Analysis

319

Amino Acids

36.66

Weight (kDa)

6.1

Isoelectric Point (pI)

35.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N_2 PF13409 41 - 147 4.4e-09 Glutathione S-transferase, N-terminal domain
GST_C PF00043 189 - 248 2.1e-07 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 195 - 246 3.4e-12 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 710
AccB1I GGYRCC 1 cut(s) 141
AccBSI CCGCTC 1 cut(s) 414
AccI GTMKAC 1 cut(s) 347
AccII CGCG 2 cut(s) 61, 467
AciI CCGC 6 cut(s) 61, 275, 414, 561, 663, 903
AclWI GGATC 1 cut(s) 846
AcsI RAATTY 3 cut(s) 70, 443, 775
AcuI CTGAAG 1 cut(s) 699
AfaI GTAC 3 cut(s) 359, 611, 759
AfiI CCNNNNNNNGG 3 cut(s) 129, 299, 811
AflIII ACRYGT 1 cut(s) 465
AgsI TTSAA 6 cut(s) 157, 298, 436, 448, 742, 784
AhdI GACNNNNNGTC 1 cut(s) 922
AjnI CCWGG 3 cut(s) 91, 137, 804
AjuI GAANNNNNNNTTGG 2 cut(s) 785, 817
AluBI AGCT 6 cut(s) 186, 271, 281, 331, 458, 617
AluI AGCT 6 cut(s) 186, 271, 281, 331, 458, 617
Alw26I GTCTC 1 cut(s) 930
AlwI GGATC 1 cut(s) 846
AlwNI CAGNNNCTG 1 cut(s) 92
AoxI GGCC 1 cut(s) 905
ApeKI GCWGC 1 cut(s) 822
ApoI RAATTY 3 cut(s) 70, 443, 775
ArsI GACNNNNNNTTYG 2 cut(s) 384, 416
Asp700I GAANNNNTTC 2 cut(s) 34, 779
AspS9I GGNCC 2 cut(s) 64, 905
AsuC2I CCSGG 1 cut(s) 813
AsuII TTCGAA 1 cut(s) 261
AsuNHI GCTAGC 1 cut(s) 271
AvaII GGWCC 1 cut(s) 64
BanI GGYRCC 1 cut(s) 141
BbvI GCAGC 1 cut(s) 834
BccI CCATC 2 cut(s) 188, 876
BcgI CGANNNNNNTGC 2 cut(s) 642, 676
BciT130I CCWGG 3 cut(s) 93, 139, 806
BclI TGATCA 1 cut(s) 508
BcnI CCSGG 1 cut(s) 813
BcoDI GTCTC 1 cut(s) 930
BfaI CTAG 2 cut(s) 272, 900
BisI GCNGC 1 cut(s) 823
BlsI GCNGC 1 cut(s) 824
BmcAI AGTACT 1 cut(s) 759
Bme1390I CCNGG 4 cut(s) 93, 139, 806, 813
Bme18I GGWCC 1 cut(s) 64
BmeRI GACNNNNNGTC 1 cut(s) 922
BmgT120I GGNCC 2 cut(s) 64, 905
BmiI GGNNCC 4 cut(s) 65, 66, 143, 810
BmrFI CCNGG 4 cut(s) 93, 139, 806, 813
BmtI GCTAGC 1 cut(s) 275
Bpu14I TTCGAA 1 cut(s) 261
BpuMI CCSGG 1 cut(s) 813
BsaAI YACGTR 1 cut(s) 730
BsaJI CCNNGG 2 cut(s) 128, 812
BsaWI WCCGGW 1 cut(s) 350
Bsc4I CCNNNNNNNGG 3 cut(s) 129, 299, 811
Bse1I ACTGG 1 cut(s) 368
BseBI CCWGG 3 cut(s) 93, 139, 806
BseDI CCNNGG 2 cut(s) 128, 812
BseGI GGATG 2 cut(s) 822, 868
BseLI CCNNNNNNNGG 3 cut(s) 129, 299, 811
BseNI ACTGG 1 cut(s) 368
BseRI GAGGAG 3 cut(s) 477, 647, 918
BseXI GCAGC 1 cut(s) 834
Bsh1236I CGCG 2 cut(s) 61, 467
BshFI GGCC 1 cut(s) 907
BshNI GGYRCC 1 cut(s) 141
BsiSI CCGG 2 cut(s) 351, 812
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 3 cut(s) 129, 299, 811
BsmAI GTCTC 1 cut(s) 930
BsmBI CGTCTC 1 cut(s) 930
BsmFI GGGAC 1 cut(s) 77
BsnI GGCC 1 cut(s) 907
Bsp119I TTCGAA 1 cut(s) 261
Bsp143I GATC 2 cut(s) 508, 838
BspACI CCGC 6 cut(s) 61, 275, 414, 561, 663, 903
BspANI GGCC 1 cut(s) 907
BspFNI CGCG 2 cut(s) 61, 467
BspLI GGNNCC 4 cut(s) 65, 66, 143, 810
BspOI GCTAGC 1 cut(s) 275
BspPI GGATC 1 cut(s) 846
BspT104I TTCGAA 1 cut(s) 261
BspT107I GGYRCC 1 cut(s) 141
BsrBI CCGCTC 1 cut(s) 414
BsrI ACTGG 1 cut(s) 368
BssECI CCNNGG 2 cut(s) 128, 812
BssMI GATC 2 cut(s) 508, 838
BssNAI GTATAC 1 cut(s) 348
BssT1I CCWWGG 1 cut(s) 128
Bst1107I GTATAC 1 cut(s) 348
Bst2UI CCWGG 3 cut(s) 93, 139, 806
Bst4CI ACNGT 4 cut(s) 345, 364, 548, 829
BstBAI YACGTR 1 cut(s) 730
BstBI TTCGAA 1 cut(s) 261
BstC8I GCNNGC 6 cut(s) 124, 273, 333, 337, 647, 905
BstDEI CTNAG 1 cut(s) 641
BstF5I GGATG 2 cut(s) 822, 868
BstFNI CGCG 2 cut(s) 61, 467
BstKTI GATC 2 cut(s) 511, 841
BstMAI GTCTC 1 cut(s) 930
BstMBI GATC 2 cut(s) 508, 838
BstMWI GCNNNNNNNGC 1 cut(s) 464
BstNI CCWGG 3 cut(s) 93, 139, 806
BstNSI RCATGY 1 cut(s) 433
BstSCI CCNGG 4 cut(s) 91, 137, 804, 811
BstSNI TACGTA 1 cut(s) 730
BstUI CGCG 2 cut(s) 61, 467
BstV1I GCAGC 1 cut(s) 834
BstXI CCANNNNNNTGG 1 cut(s) 375
BstZ17I GTATAC 1 cut(s) 348
BsuRI GGCC 1 cut(s) 907
BtsCI GGATG 2 cut(s) 822, 868
Cac8I GCNNGC 6 cut(s) 124, 273, 333, 337, 647, 905
CaiI CAGNNNCTG 1 cut(s) 92
Cfr13I GGNCC 2 cut(s) 64, 905
CseI GACGC 1 cut(s) 456
Csp6I GTAC 3 cut(s) 358, 610, 758
CviAII CATG 2 cut(s) 430, 835
CviQI GTAC 3 cut(s) 358, 610, 758
DdeI CTNAG 1 cut(s) 641
DpnI GATC 2 cut(s) 510, 840
DpnII GATC 2 cut(s) 508, 838
DriI GACNNNNNGTC 1 cut(s) 922
Eam1105I GACNNNNNGTC 1 cut(s) 922
Eco105I TACGTA 1 cut(s) 730
Eco130I CCWWGG 1 cut(s) 128
Eco32I GATATC 2 cut(s) 101, 913
Eco47I GGWCC 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 699
EcoO109I RGGNCCY 1 cut(s) 64
EcoRI GAATTC 2 cut(s) 70, 443
EcoRII CCWGG 3 cut(s) 91, 137, 804
EcoRV GATATC 2 cut(s) 101, 913
EcoT14I CCWWGG 1 cut(s) 128
ErhI CCWWGG 1 cut(s) 128
Esp3I CGTCTC 1 cut(s) 930
FaeI CATG 2 cut(s) 433, 838
FaqI GGGAC 1 cut(s) 77
FatI CATG 2 cut(s) 429, 834
FauI CCCGC 6 cut(s) 54, 268, 407, 554, 656, 896
FauNDI CATATG 2 cut(s) 118, 237
FbaI TGATCA 1 cut(s) 508
FblI GTMKAC 1 cut(s) 347
Fnu4HI GCNGC 1 cut(s) 823
FokI GGATG 2 cut(s) 829, 855
Fsp4HI GCNGC 1 cut(s) 823
FspBI CTAG 2 cut(s) 272, 900
GluI GCNGC 1 cut(s) 823
HaeIII GGCC 1 cut(s) 907
HapII CCGG 2 cut(s) 351, 812
HgaI GACGC 1 cut(s) 456
Hin1II CATG 2 cut(s) 433, 838
HincII GTYRAC 1 cut(s) 832
HindII GTYRAC 1 cut(s) 832
HindIII AAGCTT 1 cut(s) 615
HinfI GANTC 2 cut(s) 168, 714
HpaII CCGG 2 cut(s) 351, 812
Hpy166II GTNNAC 5 cut(s) 348, 360, 727, 736, 832
Hpy188I TCNGA 3 cut(s) 513, 679, 890
Hpy188III TCNNGA 1 cut(s) 932
Hpy8I GTNNAC 5 cut(s) 348, 360, 727, 736, 832
Hpy99I CGWCG 1 cut(s) 722
HpyCH4III ACNGT 4 cut(s) 345, 364, 548, 829
HpyCH4IV ACGT 2 cut(s) 191, 729
HpyCH4V TGCA 5 cut(s) 20, 149, 335, 496, 747
HpyF10VI GCNNNNNNNGC 1 cut(s) 464
HpyF3I CTNAG 1 cut(s) 641
HpySE526I ACGT 2 cut(s) 191, 729
Hsp92II CATG 2 cut(s) 433, 838
KflI GGGWCCC 1 cut(s) 64
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 2 cut(s) 508, 838
LmnI GCTCC 2 cut(s) 278, 302
Lsp1109I GCAGC 1 cut(s) 834
MaeI CTAG 2 cut(s) 272, 900
MaeII ACGT 2 cut(s) 191, 729
MaeIII GTNAC 2 cut(s) 47, 700
MalI GATC 2 cut(s) 510, 840
MbiI CCGCTC 1 cut(s) 414
MboI GATC 2 cut(s) 508, 838
MboII GAAGA 1 cut(s) 108
MfeI CAATTG 2 cut(s) 396, 605
MluCI AATT 8 cut(s) 70, 77, 396, 443, 504, 605, 775, 894
MluI ACGCGT 1 cut(s) 465
MlyI GAGTC 1 cut(s) 162
MnlI CCTC 8 cut(s) 16, 495, 498, 564, 586, 625, 715, 939
MroXI GAANNNNTTC 2 cut(s) 34, 779
MspI CCGG 2 cut(s) 351, 812
MspR9I CCNGG 4 cut(s) 93, 139, 806, 813
MunI CAATTG 2 cut(s) 396, 605
MvaI CCWGG 3 cut(s) 93, 139, 806
MvnI CGCG 2 cut(s) 61, 467
MwoI GCNNNNNNNGC 1 cut(s) 464
NciI CCSGG 1 cut(s) 813
NdeI CATATG 2 cut(s) 118, 237
NdeII GATC 2 cut(s) 508, 838
NheI GCTAGC 1 cut(s) 271
NlaIII CATG 2 cut(s) 433, 838
NlaIV GGNNCC 4 cut(s) 65, 66, 143, 810
NmuCI GTSAC 1 cut(s) 700
NspI RCATGY 1 cut(s) 433
NspV TTCGAA 1 cut(s) 261
PcsI WCGNNNNNNNCGW 2 cut(s) 65, 726
PdmI GAANNNNTTC 2 cut(s) 34, 779
PfeI GAWTC 1 cut(s) 714
PkrI GCNGC 1 cut(s) 824
PleI GAGTC 1 cut(s) 162
PpsI GAGTC 1 cut(s) 162
Ppu21I YACGTR 1 cut(s) 730
PpuMI RGGWCCY 1 cut(s) 64
PsiI TTATAA 1 cut(s) 710
Psp5II RGGWCCY 1 cut(s) 64
Psp6I CCWGG 3 cut(s) 91, 137, 804
PspGI CCWGG 3 cut(s) 91, 137, 804
PspN4I GGNNCC 4 cut(s) 65, 66, 143, 810
PspPI GGNCC 2 cut(s) 64, 905
PspPPI RGGWCCY 1 cut(s) 64
PstNI CAGNNNCTG 1 cut(s) 92
RsaI GTAC 3 cut(s) 359, 611, 759
RsaNI GTAC 3 cut(s) 358, 610, 758
SatI GCNGC 1 cut(s) 823
Sau3AI GATC 2 cut(s) 508, 838
Sau96I GGNCC 2 cut(s) 64, 905
ScaI AGTACT 1 cut(s) 759
SchI GAGTC 1 cut(s) 162
ScrFI CCNGG 4 cut(s) 93, 139, 806, 813
SfuI TTCGAA 1 cut(s) 261
SinI GGWCC 1 cut(s) 64
SnaBI TACGTA 1 cut(s) 730
Sse9I AATT 8 cut(s) 70, 77, 396, 443, 504, 605, 775, 894
SsiI CCGC 6 cut(s) 61, 275, 414, 561, 663, 903
SspMI CTAG 2 cut(s) 272, 900
StyD4I CCNGG 4 cut(s) 91, 137, 804, 811
StyI CCWWGG 1 cut(s) 128
TaaI ACNGT 4 cut(s) 345, 364, 548, 829
TaiI ACGT 2 cut(s) 194, 732
TaqI TCGA 5 cut(s) 171, 261, 717, 915, 950
TasI AATT 8 cut(s) 70, 77, 396, 443, 504, 605, 775, 894
TatI WGTACW 2 cut(s) 357, 757
TfiI GAWTC 1 cut(s) 714
TseFI GTSAC 1 cut(s) 700
TseI GCWGC 1 cut(s) 822
Tsp45I GTSAC 1 cut(s) 700
TspDTI ATGAA 3 cut(s) 43, 246, 534
TspGWI ACGGA 2 cut(s) 861, 876
VpaK11BI GGWCC 1 cut(s) 64
XapI RAATTY 3 cut(s) 70, 443, 775
XceI RCATGY 1 cut(s) 433
XmiI GTMKAC 1 cut(s) 347
XmnI GAANNNNTTC 2 cut(s) 34, 779
XspI CTAG 2 cut(s) 272, 900
ZrmI AGTACT 1 cut(s) 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.