Rroxscaffold_1G00058130

Glutathione S-transferase omega-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005011
Physical Location & Seq
Forward (+)
80014157 .. 80019093
4937 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00058130.1

Sequence Viewer

Length: 1035 bp
ATGAATAGACTCGGTTGGAGAATTTTCAGCAAAAATAATCCACTGGCACACTATCACATGGCTCGGTCTGCACTGGATGAGGTCTCACAATCTGGTGCATTTGTGAGAAGTGCTTCTGAATTCCGTAACTTCATTTCACGAGACCCGAACTCGCAATTTCCAGCAGAAGCTGGGAGATATCATCTGTATGTATCATATGCTTGCCCTTGGGCGTCAAGGTGCCTTGCATACTTGAATATTAAAGGACTTCAGAAAGCTATCAGCTTTACGTCAGTCAAACCTATATGGGAAAGAACAAAAGAAAGTGATGAGCATATGGGATGGGTTTTTCCTGCTTCAGATAAGGAGCTAGAAGGAGCTGAACCTGACCCTTTGAATGGAGCAAAAAGTATACGAGAACTTTATGAGATTGCTAGTACAAAATACACTGGAAAGTACACTGTTCCGGTTCTGTGGGATAAAAAACTTGGAACAATTGTCAGTAATGAGAGTTCGGAGATTATTCGCATGTTTAATACTGAATTCAACAGTATAGCAGAGAATCCTGCTTTGGACTTGTATCCCGATCACTTGCAATCCCAAGTCAATCAGACTAATGAATGGATATACGACAAGATAAATAACGGTGTCTATAAATGTGGGTTTGCCAAGAAGCAGGAGCCGTATGATGAGGCTGTGAAACAGTTGTATGAAGCTTTGGACAGATGTGAGGAGATACTTAGCAAGCAACGATACCTCTGTGGAAACACATTATCTGAAGCAGATATTCGTTTGTTTGTGACTCTCGTAAGATTTGATGAGGTTTATACAGTCCACTTCAAGTGCAACAAGAAACTCCTGCGAGAGTACCCAAATCTGTTTAACTACACCAAGGACATTTTCCAAGTTCCTGGAATGAGCAGCACAGTCCAGATGGATCACATCAAACGTCACTACTATGGAAGCCATCCTTCTATCAATCCATTCGGGATAGTTCCGTCCGGCCCAAACATCGACTACTCTTCTCCTCATGACAGAAACAAGTTTTCTGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GST_C_2 GST_N_2

Protein Analysis

344

Amino Acids

39.62

Weight (kDa)

7.14

Isoelectric Point (pI)

45.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N_2 PF13409 67 - 173 4.6e-10 Glutathione S-transferase, N-terminal domain
GST_C PF00043 215 - 295 2.3e-09 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 221 - 290 8.5e-14 Glutathione S-transferase, C-terminal domain
GST_C_3 PF14497 224 - 298 2.8e-07 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 219
AccI GTMKAC 1 cut(s) 391
AclWI GGATC 1 cut(s) 924
AcsI RAATTY 3 cut(s) 21, 119, 521
AcuI CTGAAG 3 cut(s) 233, 321, 777
AcyI GRCGYC 1 cut(s) 212
AfaI GTAC 3 cut(s) 418, 437, 848
AfiI CCNNNNNNNGG 1 cut(s) 377
AgsI TTSAA 4 cut(s) 235, 376, 526, 820
AjnI CCWGG 1 cut(s) 889
AjuI GAANNNNNNNTTGG 4 cut(s) 533, 565, 863, 895
AluBI AGCT 6 cut(s) 170, 257, 264, 349, 359, 695
AluI AGCT 6 cut(s) 170, 257, 264, 349, 359, 695
Alw26I GTCTC 2 cut(s) 88, 135
AlwI GGATC 1 cut(s) 924
AlwNI CAGNNNCTG 1 cut(s) 170
AoxI GGCC 1 cut(s) 982
ApeKI GCWGC 1 cut(s) 900
ApoI RAATTY 3 cut(s) 21, 119, 521
Asp700I GAANNNNTTC 1 cut(s) 112
AspS9I GGNCC 1 cut(s) 983
BaeI ACNNNNGTAYC 2 cut(s) 724, 757
BanI GGYRCC 1 cut(s) 219
BauI CACGAG 1 cut(s) 138
BbvI GCAGC 1 cut(s) 912
BccI CCATC 3 cut(s) 315, 907, 954
BceAI ACGGC 1 cut(s) 646
BciT130I CCWGG 1 cut(s) 891
BciVI GTATCC 1 cut(s) 570
BcoDI GTCTC 2 cut(s) 88, 135
BfaI CTAG 2 cut(s) 350, 414
BfuI GTATCC 1 cut(s) 570
BisI GCNGC 1 cut(s) 901
BlsI GCNGC 1 cut(s) 902
Bme1390I CCNGG 1 cut(s) 891
BmgT120I GGNCC 1 cut(s) 983
BmiI GGNNCC 2 cut(s) 221, 660
BmrFI CCNGG 1 cut(s) 891
BsaHI GRCGYC 1 cut(s) 212
BsaI GGTCTC 2 cut(s) 88, 135
BsaJI CCNNGG 2 cut(s) 206, 870
BsaWI WCCGGW 1 cut(s) 445
Bsc4I CCNNNNNNNGG 1 cut(s) 377
Bse1I ACTGG 3 cut(s) 48, 78, 433
BseBI CCWGG 1 cut(s) 891
BseDI CCNNGG 2 cut(s) 206, 870
BseGI GGATG 3 cut(s) 82, 326, 946
BseLI CCNNNNNNNGG 1 cut(s) 377
BseNI ACTGG 3 cut(s) 48, 78, 433
BseRI GAGGAG 2 cut(s) 725, 996
BseXI GCAGC 1 cut(s) 912
BseYI CCCAGC 1 cut(s) 170
BsgI GTGCAG 1 cut(s) 54
BshFI GGCC 1 cut(s) 984
BshNI GGYRCC 1 cut(s) 219
BsiSI CCGG 2 cut(s) 446, 981
BslI CCNNNNNNNGG 1 cut(s) 377
BsmAI GTCTC 2 cut(s) 88, 135
BsnI GGCC 1 cut(s) 984
Bso31I GGTCTC 2 cut(s) 88, 135
Bsp143I GATC 2 cut(s) 565, 916
BspANI GGCC 1 cut(s) 984
BspHI TCATGA 1 cut(s) 1009
BspLI GGNNCC 2 cut(s) 221, 660
BspPI GGATC 1 cut(s) 924
BspT107I GGYRCC 1 cut(s) 219
BspTNI GGTCTC 2 cut(s) 88, 135
BsrI ACTGG 3 cut(s) 48, 78, 433
BssECI CCNNGG 2 cut(s) 206, 870
BssMI GATC 2 cut(s) 565, 916
BssNAI GTATAC 1 cut(s) 392
BssNI GRCGYC 1 cut(s) 212
BssSI CACGAG 1 cut(s) 138
BssT1I CCWWGG 2 cut(s) 206, 870
Bst1107I GTATAC 1 cut(s) 392
Bst2BI CACGAG 1 cut(s) 138
Bst2UI CCWGG 1 cut(s) 891
Bst4CI ACNGT 6 cut(s) 442, 530, 626, 684, 811, 907
Bst6I CTCTTC 1 cut(s) 1006
BstACI GRCGYC 1 cut(s) 212
BstC8I GCNNGC 2 cut(s) 202, 725
BstDEI CTNAG 1 cut(s) 719
BstF5I GGATG 3 cut(s) 82, 326, 946
BstKTI GATC 2 cut(s) 568, 919
BstMAI GTCTC 2 cut(s) 88, 135
BstMBI GATC 2 cut(s) 565, 916
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstNI CCWGG 1 cut(s) 891
BstNSI RCATGY 1 cut(s) 511
BstSCI CCNGG 1 cut(s) 889
BstV1I GCAGC 1 cut(s) 912
BstXI CCANNNNNNTGG 1 cut(s) 890
BstZ17I GTATAC 1 cut(s) 392
BsuI GTATCC 1 cut(s) 570
BsuRI GGCC 1 cut(s) 984
BtsCI GGATG 3 cut(s) 82, 326, 946
BtsIMutI CAGTG 4 cut(s) 41, 71, 426, 438
Cac8I GCNNGC 2 cut(s) 202, 725
CaiI CAGNNNCTG 1 cut(s) 170
CciI TCATGA 1 cut(s) 1009
Cfr13I GGNCC 1 cut(s) 983
CseI GACGC 1 cut(s) 201
Csp6I GTAC 3 cut(s) 417, 436, 847
CviAII CATG 3 cut(s) 58, 508, 1010
CviQI GTAC 3 cut(s) 417, 436, 847
DdeI CTNAG 1 cut(s) 719
DpnI GATC 2 cut(s) 567, 918
DpnII GATC 2 cut(s) 565, 916
Eam1104I CTCTTC 1 cut(s) 1006
EarI CTCTTC 1 cut(s) 1006
Eco130I CCWWGG 2 cut(s) 206, 870
Eco31I GGTCTC 2 cut(s) 88, 135
Eco32I GATATC 1 cut(s) 179
Eco57I CTGAAG 3 cut(s) 233, 321, 777
EcoRI GAATTC 2 cut(s) 119, 521
EcoRII CCWGG 1 cut(s) 889
EcoRV GATATC 1 cut(s) 179
EcoT14I CCWWGG 2 cut(s) 206, 870
ErhI CCWWGG 2 cut(s) 206, 870
FaeI CATG 3 cut(s) 61, 511, 1013
FalI AAGNNNNNCTT 2 cut(s) 934, 966
FatI CATG 3 cut(s) 57, 507, 1009
FauNDI CATATG 2 cut(s) 196, 315
FblI GTMKAC 1 cut(s) 391
Fnu4HI GCNGC 1 cut(s) 901
FokI GGATG 3 cut(s) 89, 333, 933
Fsp4HI GCNGC 1 cut(s) 901
FspBI CTAG 2 cut(s) 350, 414
GluI GCNGC 1 cut(s) 901
GsaI CCCAGC 1 cut(s) 174
HaeIII GGCC 1 cut(s) 984
HapII CCGG 2 cut(s) 446, 981
HgaI GACGC 1 cut(s) 201
Hin1I GRCGYC 1 cut(s) 212
Hin1II CATG 3 cut(s) 61, 511, 1013
HindIII AAGCTT 1 cut(s) 693
HinfI GANTC 3 cut(s) 9, 541, 781
HpaII CCGG 2 cut(s) 446, 981
Hpy166II GTNNAC 3 cut(s) 392, 438, 814
Hpy188I TCNGA 6 cut(s) 118, 252, 340, 496, 591, 757
Hpy188III TCNNGA 5 cut(s) 138, 563, 910, 967, 1010
Hpy8I GTNNAC 3 cut(s) 392, 438, 814
HpyAV CCTTC 2 cut(s) 347, 960
HpyCH4III ACNGT 6 cut(s) 442, 530, 626, 684, 811, 907
HpyCH4IV ACGT 2 cut(s) 269, 928
HpyCH4V TGCA 5 cut(s) 71, 98, 227, 574, 825
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
HpyF3I CTNAG 1 cut(s) 719
HpySE526I ACGT 2 cut(s) 269, 928
Hsp92I GRCGYC 1 cut(s) 212
Hsp92II CATG 3 cut(s) 61, 511, 1013
Kzo9I GATC 2 cut(s) 565, 916
LmnI GCTCC 4 cut(s) 346, 356, 380, 658
Lsp1109I GCAGC 1 cut(s) 912
MaeI CTAG 2 cut(s) 350, 414
MaeII ACGT 2 cut(s) 269, 928
MaeIII GTNAC 3 cut(s) 125, 778, 929
MalI GATC 2 cut(s) 567, 918
MboI GATC 2 cut(s) 565, 916
MboII GAAGA 1 cut(s) 993
MfeI CAATTG 1 cut(s) 474
MluCI AATT 5 cut(s) 21, 119, 155, 474, 521
MlyI GAGTC 2 cut(s) 3, 775
MnlI CCTC 6 cut(s) 73, 664, 703, 746, 793, 1017
MroXI GAANNNNTTC 1 cut(s) 112
MseI TTAA 3 cut(s) 240, 513, 861
MslI CAYNNNNRTG 2 cut(s) 186, 936
MspI CCGG 2 cut(s) 446, 981
MspR9I CCNGG 1 cut(s) 891
MunI CAATTG 1 cut(s) 474
MvaI CCWGG 1 cut(s) 891
MwoI GCNNNNNNNGC 1 cut(s) 68
NdeI CATATG 2 cut(s) 196, 315
NdeII GATC 2 cut(s) 565, 916
NlaIII CATG 3 cut(s) 61, 511, 1013
NlaIV GGNNCC 2 cut(s) 221, 660
NmuCI GTSAC 2 cut(s) 778, 929
NspI RCATGY 1 cut(s) 511
PagI TCATGA 1 cut(s) 1009
PdmI GAANNNNTTC 1 cut(s) 112
PfeI GAWTC 1 cut(s) 541
PfoI TCCNGGA 1 cut(s) 889
PkrI GCNGC 1 cut(s) 902
PleI GAGTC 2 cut(s) 3, 775
PpsI GAGTC 2 cut(s) 3, 775
Psp6I CCWGG 1 cut(s) 889
PspFI CCCAGC 1 cut(s) 170
PspGI CCWGG 1 cut(s) 889
PspN4I GGNNCC 2 cut(s) 221, 660
PspPI GGNCC 1 cut(s) 983
PsrI GAACNNNNNNTAC 2 cut(s) 475, 507
PstNI CAGNNNCTG 1 cut(s) 170
RsaI GTAC 3 cut(s) 418, 437, 848
RsaNI GTAC 3 cut(s) 417, 436, 847
RseI CAYNNNNRTG 2 cut(s) 186, 936
SaqAI TTAA 3 cut(s) 240, 513, 861
SatI GCNGC 1 cut(s) 901
Sau3AI GATC 2 cut(s) 565, 916
Sau96I GGNCC 1 cut(s) 983
SchI GAGTC 2 cut(s) 3, 775
ScrFI CCNGG 1 cut(s) 891
SmiMI CAYNNNNRTG 2 cut(s) 186, 936
Sse9I AATT 5 cut(s) 21, 119, 155, 474, 521
SspI AATATT 1 cut(s) 238
SspMI CTAG 2 cut(s) 350, 414
StyD4I CCNGG 1 cut(s) 889
StyI CCWWGG 2 cut(s) 206, 870
TaaI ACNGT 6 cut(s) 442, 530, 626, 684, 811, 907
TaiI ACGT 2 cut(s) 272, 931
TaqI TCGA 1 cut(s) 993
TaqII GACCGA 1 cut(s) 54
TasI AATT 5 cut(s) 21, 119, 155, 474, 521
TatI WGTACW 2 cut(s) 416, 435
TfiI GAWTC 1 cut(s) 541
Tru1I TTAA 3 cut(s) 240, 513, 861
Tru9I TTAA 3 cut(s) 240, 513, 861
TscAI CASTG 4 cut(s) 48, 78, 433, 445
TseFI GTSAC 2 cut(s) 778, 929
TseI GCWGC 1 cut(s) 900
Tsp45I GTSAC 2 cut(s) 778, 929
TspDTI ATGAA 4 cut(s) 17, 121, 612, 705
TspGWI ACGGA 2 cut(s) 113, 966
TspRI CASTG 4 cut(s) 48, 78, 433, 445
XapI RAATTY 3 cut(s) 21, 119, 521
XceI RCATGY 1 cut(s) 511
XmiI GTMKAC 1 cut(s) 391
XmnI GAANNNNTTC 1 cut(s) 112
XspI CTAG 2 cut(s) 350, 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.