MD10G1233400.v1.1

Glutathione S-transferase omega-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005011
Physical Location & Seq
Forward (+)
32935100 .. 32937902
2803 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1233400.v1.1.491

Sequence Viewer

Length: 978 bp
ATGGCGCGATCTGCACTAGATGAGACCTCACTATCGGGTGCATTTGTGAGAACTGCTTCTGTATTTCGTAACTTCATCTCGCGGGACCCGAATTCGCAATTTCCAGCAGAACCTGGAAGATATCATCTGTATATATCATATGCTTGCCCTTGGGCTTCCAGGTGCCTTGCGTACTTGAACATAAAAGGACTTGATAAAGCCATCAGCTTTACGTCAGTCAAACCGATATGGGAAAGGACAAAAGAAAGTGATGAACATATGGGGTGGGTTTTTCCTGCTTCCGAAACAGAGCTAGCGGGAGCTGAACCCGACCCCTTGAATGGAGCAAAAAGCATTAGAGAACTTTACGAGCTTGCAAGCACACAGTATACCGGAAAGTACACGGTTCCAGTTCTATGGGATAAAAAACTCAAAACAATTGTTAGTAATGAGAGCGGGGAGATTATTCGCATGTTCAATACTGAATTCAACGATATAGCTGAAAACGCATCTTTGGACTTATATCCTCCTCACTTGCAATCCCAAATTGATCAGACAAATGAATGGATATATGACAAGATAAACAACGGTGTCTATAAATGCGGGTTTGCGAGGAAGCAAGAGCCTTATGATGAGGCTGTGAAACAATTGTACGAAGCTTTGGATAAATGTGAGGAGATACTTAGCAAGCAACGATACCTGTGCGGGAACACATTGTCTGAAGCAGATATTCGGTTGTTTGTCACTCTTATAAGATTCGACGAGGTTTACGTAGTCCACTTCAAGTGCAACAAGAAGGTACTACGAGAATACCCGAATTTGTTCAACTACACCAAAGACATTTTCCAGGTTCCGGGGATGAGCAGCACAGTCAACATGGATCACATAAAGCGGCACTACTACGGAAGCCATCCGTCTATCAACCCATTCGGAATAATTCCTAGCGGGCCAGATATCGACTACTCTTCTCCTCATGATAGAAACAGGTTTTCGACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GST_C_2 GST_N_2

Protein Analysis

326

Amino Acids

37.33

Weight (kDa)

5.95

Isoelectric Point (pI)

38.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N_2 PF13409 48 - 154 4.6e-09 Glutathione S-transferase, N-terminal domain
GST_C PF00043 196 - 276 5.2e-09 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 202 - 271 4.4e-14 Glutathione S-transferase, C-terminal domain
GST_C_3 PF14497 207 - 279 4e-07 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 731
AccB1I GGYRCC 1 cut(s) 162
AccBSI CCGCTC 1 cut(s) 435
AccI GTMKAC 1 cut(s) 368
AccII CGCG 2 cut(s) 7, 82
AciI CCGC 7 cut(s) 82, 296, 435, 582, 684, 871, 924
AclWI GGATC 1 cut(s) 867
AcsI RAATTY 3 cut(s) 91, 464, 796
AcuI CTGAAG 1 cut(s) 720
AfaI GTAC 4 cut(s) 173, 380, 632, 780
AfiI CCNNNNNNNGG 2 cut(s) 320, 832
AgsI TTSAA 6 cut(s) 178, 319, 457, 469, 763, 805
AjnI CCWGG 3 cut(s) 112, 158, 825
AjuI GAANNNNNNNTTGG 2 cut(s) 806, 838
AluBI AGCT 6 cut(s) 207, 292, 302, 352, 479, 638
AluI AGCT 6 cut(s) 207, 292, 302, 352, 479, 638
Alw26I GTCTC 1 cut(s) 17
AlwI GGATC 1 cut(s) 867
AlwNI CAGNNNCTG 1 cut(s) 113
AoxI GGCC 1 cut(s) 926
ApeKI GCWGC 1 cut(s) 843
ApoI RAATTY 3 cut(s) 91, 464, 796
Asp700I GAANNNNTTC 2 cut(s) 55, 800
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 2 cut(s) 85, 926
AsuC2I CCSGG 1 cut(s) 834
AsuNHI GCTAGC 1 cut(s) 292
AvaII GGWCC 1 cut(s) 85
BanI GGYRCC 1 cut(s) 162
BbvI GCAGC 1 cut(s) 855
BccI CCATC 2 cut(s) 209, 897
BcgI CGANNNNNNTGC 2 cut(s) 663, 697
BciT130I CCWGG 3 cut(s) 114, 160, 827
BclI TGATCA 1 cut(s) 529
BcnI CCSGG 1 cut(s) 834
BcoDI GTCTC 1 cut(s) 17
BfaI CTAG 3 cut(s) 17, 293, 921
BisI GCNGC 2 cut(s) 844, 872
BlsI GCNGC 2 cut(s) 845, 873
Bme1390I CCNGG 4 cut(s) 114, 160, 827, 834
Bme18I GGWCC 1 cut(s) 85
BmgT120I GGNCC 2 cut(s) 85, 926
BmiI GGNNCC 5 cut(s) 86, 87, 164, 387, 831
BmrFI CCNGG 4 cut(s) 114, 160, 827, 834
BmsI GCATC 1 cut(s) 497
BmtI GCTAGC 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 834
BsaAI YACGTR 1 cut(s) 751
BsaI GGTCTC 1 cut(s) 17
BsaJI CCNNGG 2 cut(s) 149, 833
BsaWI WCCGGW 1 cut(s) 371
Bsc4I CCNNNNNNNGG 2 cut(s) 320, 832
Bse1I ACTGG 1 cut(s) 389
BseBI CCWGG 3 cut(s) 114, 160, 827
BseDI CCNNGG 2 cut(s) 149, 833
BseGI GGATG 2 cut(s) 843, 889
BseLI CCNNNNNNNGG 2 cut(s) 320, 832
BseNI ACTGG 1 cut(s) 389
BseRI GAGGAG 3 cut(s) 498, 668, 939
BseXI GCAGC 1 cut(s) 855
Bsh1236I CGCG 2 cut(s) 7, 82
BshFI GGCC 1 cut(s) 928
BshNI GGYRCC 1 cut(s) 162
BsiSI CCGG 2 cut(s) 372, 833
BslFI GGGAC 1 cut(s) 98
BslI CCNNNNNNNGG 2 cut(s) 320, 832
BsmAI GTCTC 1 cut(s) 17
BsmFI GGGAC 1 cut(s) 98
BsnI GGCC 1 cut(s) 928
Bso31I GGTCTC 1 cut(s) 17
Bsp143I GATC 3 cut(s) 8, 529, 859
BspACI CCGC 7 cut(s) 82, 296, 435, 582, 684, 871, 924
BspANI GGCC 1 cut(s) 928
BspFNI CGCG 2 cut(s) 7, 82
BspHI TCATGA 1 cut(s) 952
BspLI GGNNCC 5 cut(s) 86, 87, 164, 387, 831
BspOI GCTAGC 1 cut(s) 296
BspPI GGATC 1 cut(s) 867
BspT107I GGYRCC 1 cut(s) 162
BspTNI GGTCTC 1 cut(s) 17
BsrBI CCGCTC 1 cut(s) 435
BsrI ACTGG 1 cut(s) 389
BssECI CCNNGG 2 cut(s) 149, 833
BssMI GATC 3 cut(s) 8, 529, 859
BssNAI GTATAC 1 cut(s) 369
BssT1I CCWWGG 1 cut(s) 149
Bst1107I GTATAC 1 cut(s) 369
Bst2UI CCWGG 3 cut(s) 114, 160, 827
Bst4CI ACNGT 4 cut(s) 366, 385, 569, 850
Bst6I CTCTTC 1 cut(s) 949
BstBAI YACGTR 1 cut(s) 751
BstC8I GCNNGC 6 cut(s) 145, 294, 354, 358, 668, 926
BstDEI CTNAG 1 cut(s) 662
BstF5I GGATG 2 cut(s) 843, 889
BstFNI CGCG 2 cut(s) 7, 82
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 3 cut(s) 11, 532, 862
BstMAI GTCTC 1 cut(s) 17
BstMBI GATC 3 cut(s) 8, 529, 859
BstMWI GCNNNNNNNGC 2 cut(s) 11, 485
BstNI CCWGG 3 cut(s) 114, 160, 827
BstNSI RCATGY 1 cut(s) 454
BstSCI CCNGG 4 cut(s) 112, 158, 825, 832
BstSNI TACGTA 1 cut(s) 751
BstUI CGCG 2 cut(s) 7, 82
BstV1I GCAGC 1 cut(s) 855
BstXI CCANNNNNNTGG 1 cut(s) 396
BstZ17I GTATAC 1 cut(s) 369
BsuRI GGCC 1 cut(s) 928
BtsCI GGATG 2 cut(s) 843, 889
Cac8I GCNNGC 6 cut(s) 145, 294, 354, 358, 668, 926
CaiI CAGNNNCTG 1 cut(s) 113
CciI TCATGA 1 cut(s) 952
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 2 cut(s) 85, 926
Csp6I GTAC 4 cut(s) 172, 379, 631, 779
CviAII CATG 3 cut(s) 451, 856, 953
CviQI GTAC 4 cut(s) 172, 379, 631, 779
DdeI CTNAG 1 cut(s) 662
DpnI GATC 3 cut(s) 10, 531, 861
DpnII GATC 3 cut(s) 8, 529, 859
Eam1104I CTCTTC 1 cut(s) 949
EarI CTCTTC 1 cut(s) 949
Eco105I TACGTA 1 cut(s) 751
Eco130I CCWWGG 1 cut(s) 149
Eco31I GGTCTC 1 cut(s) 17
Eco32I GATATC 2 cut(s) 122, 934
Eco47I GGWCC 1 cut(s) 85
Eco57I CTGAAG 1 cut(s) 720
EcoO109I RGGNCCY 1 cut(s) 85
EcoRI GAATTC 2 cut(s) 91, 464
EcoRII CCWGG 3 cut(s) 112, 158, 825
EcoRV GATATC 2 cut(s) 122, 934
EcoT14I CCWWGG 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 149
FaeI CATG 3 cut(s) 454, 859, 956
FaqI GGGAC 1 cut(s) 98
FatI CATG 3 cut(s) 450, 855, 952
FauI CCCGC 6 cut(s) 75, 289, 428, 575, 677, 917
FauNDI CATATG 2 cut(s) 139, 258
FbaI TGATCA 1 cut(s) 529
FblI GTMKAC 1 cut(s) 368
Fnu4HI GCNGC 2 cut(s) 844, 872
FokI GGATG 2 cut(s) 850, 876
Fsp4HI GCNGC 2 cut(s) 844, 872
FspBI CTAG 3 cut(s) 17, 293, 921
GlaI GCGC 1 cut(s) 6
GluI GCNGC 2 cut(s) 844, 872
HaeIII GGCC 1 cut(s) 928
HapII CCGG 2 cut(s) 372, 833
HhaI GCGC 1 cut(s) 7
Hin1II CATG 3 cut(s) 454, 859, 956
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 853
HindII GTYRAC 1 cut(s) 853
HindIII AAGCTT 1 cut(s) 636
HinfI GANTC 1 cut(s) 735
HpaII CCGG 2 cut(s) 372, 833
Hpy166II GTNNAC 5 cut(s) 369, 381, 748, 757, 853
Hpy188I TCNGA 4 cut(s) 283, 534, 700, 911
Hpy188III TCNNGA 1 cut(s) 953
Hpy8I GTNNAC 5 cut(s) 369, 381, 748, 757, 853
Hpy99I CGWCG 1 cut(s) 743
HpyAV CCTTC 1 cut(s) 769
HpyCH4III ACNGT 4 cut(s) 366, 385, 569, 850
HpyCH4IV ACGT 2 cut(s) 212, 750
HpyCH4V TGCA 5 cut(s) 14, 41, 356, 517, 768
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 485
HpyF3I CTNAG 1 cut(s) 662
HpySE526I ACGT 2 cut(s) 212, 750
Hsp92II CATG 3 cut(s) 454, 859, 956
HspAI GCGC 1 cut(s) 5
KflI GGGWCCC 1 cut(s) 85
Ksp22I TGATCA 1 cut(s) 529
Kzo9I GATC 3 cut(s) 8, 529, 859
LmnI GCTCC 2 cut(s) 299, 323
Lsp1109I GCAGC 1 cut(s) 855
LweI GCATC 1 cut(s) 497
MaeI CTAG 3 cut(s) 17, 293, 921
MaeII ACGT 2 cut(s) 212, 750
MaeIII GTNAC 2 cut(s) 68, 721
MalI GATC 3 cut(s) 10, 531, 861
MbiI CCGCTC 1 cut(s) 435
MboI GATC 3 cut(s) 8, 529, 859
MboII GAAGA 2 cut(s) 129, 936
MfeI CAATTG 2 cut(s) 417, 626
MluCI AATT 8 cut(s) 91, 98, 417, 464, 525, 626, 796, 915
MnlI CCTC 8 cut(s) 37, 516, 519, 585, 607, 646, 736, 960
MroXI GAANNNNTTC 2 cut(s) 55, 800
MspI CCGG 2 cut(s) 372, 833
MspR9I CCNGG 4 cut(s) 114, 160, 827, 834
MunI CAATTG 2 cut(s) 417, 626
MvaI CCWGG 3 cut(s) 114, 160, 827
MvnI CGCG 2 cut(s) 7, 82
MwoI GCNNNNNNNGC 2 cut(s) 11, 485
NciI CCSGG 1 cut(s) 834
NdeI CATATG 2 cut(s) 139, 258
NdeII GATC 3 cut(s) 8, 529, 859
NheI GCTAGC 1 cut(s) 292
NlaIII CATG 3 cut(s) 454, 859, 956
NlaIV GGNNCC 5 cut(s) 86, 87, 164, 387, 831
NmuCI GTSAC 1 cut(s) 721
NspI RCATGY 1 cut(s) 454
PagI TCATGA 1 cut(s) 952
PcsI WCGNNNNNNNCGW 2 cut(s) 86, 747
PdmI GAANNNNTTC 2 cut(s) 55, 800
PfeI GAWTC 1 cut(s) 735
PkrI GCNGC 2 cut(s) 845, 873
Ppu21I YACGTR 1 cut(s) 751
PpuMI RGGWCCY 1 cut(s) 85
PsiI TTATAA 1 cut(s) 731
Psp5II RGGWCCY 1 cut(s) 85
Psp6I CCWGG 3 cut(s) 112, 158, 825
PspGI CCWGG 3 cut(s) 112, 158, 825
PspN4I GGNNCC 5 cut(s) 86, 87, 164, 387, 831
PspPI GGNCC 2 cut(s) 85, 926
PspPPI RGGWCCY 1 cut(s) 85
PstNI CAGNNNCTG 1 cut(s) 113
RsaI GTAC 4 cut(s) 173, 380, 632, 780
RsaNI GTAC 4 cut(s) 172, 379, 631, 779
SatI GCNGC 2 cut(s) 844, 872
Sau3AI GATC 3 cut(s) 8, 529, 859
Sau96I GGNCC 2 cut(s) 85, 926
ScrFI CCNGG 4 cut(s) 114, 160, 827, 834
SfaNI GCATC 1 cut(s) 497
SinI GGWCC 1 cut(s) 85
SnaBI TACGTA 1 cut(s) 751
Sse9I AATT 8 cut(s) 91, 98, 417, 464, 525, 626, 796, 915
SsiI CCGC 7 cut(s) 82, 296, 435, 582, 684, 871, 924
SspMI CTAG 3 cut(s) 17, 293, 921
StyD4I CCNGG 4 cut(s) 112, 158, 825, 832
StyI CCWWGG 1 cut(s) 149
TaaI ACNGT 4 cut(s) 366, 385, 569, 850
TaiI ACGT 2 cut(s) 215, 753
TaqI TCGA 3 cut(s) 738, 936, 971
TasI AATT 8 cut(s) 91, 98, 417, 464, 525, 626, 796, 915
TatI WGTACW 1 cut(s) 378
TauI GCSGC 1 cut(s) 874
TfiI GAWTC 1 cut(s) 735
TseFI GTSAC 1 cut(s) 721
TseI GCWGC 1 cut(s) 843
Tsp45I GTSAC 1 cut(s) 721
TspDTI ATGAA 3 cut(s) 64, 267, 555
TspGWI ACGGA 2 cut(s) 882, 897
VpaK11BI GGWCC 1 cut(s) 85
XapI RAATTY 3 cut(s) 91, 464, 796
XceI RCATGY 1 cut(s) 454
XmiI GTMKAC 1 cut(s) 368
XmnI GAANNNNTTC 2 cut(s) 55, 800
XspI CTAG 3 cut(s) 17, 293, 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.