FvH4_3g13890

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012861
Physical Location & Seq
Reverse (-)
8351997 .. 8354932
2936 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g13890.t1

Sequence Viewer

Length: 1233 bp
ATGTGCAGCTCTAAGGCCAAGGTGACCATGGGGATTGAAATCACACCCCTTGTTTCTAGAATCAATGGCAGACCAGTTCTTCAGCCTACTTGTAACCGAGTTCCTAGCCTAGACCGGCGCAATTCCCTCAAGAAACTATCAACACCACCACCACCACCTCTACCATTATCAAATGCTTCTTCTACGAGCACTAGTCCTAGAATTAGTACCAAGGCTTCATTGACAACACCACCAGTATCTCCCAAGTCAAAATCTCCAAGACCGCCTGCGATAAAGAGATCAGGCAATGACCCTAATGGCTTGAACTCCAGTTCTGAGAAAGTTGTGACTCCAGGAGGTACCACAAGAGCTAAGGTTTTGGAAAGGAAGAAGTCAAAGAGTTTTAAGCTTGGTGTTGGTGCTGATAATGCTCATGACCATGGTAGACTTTCTTCTGCTTCGATTGAGGCTTCGTTGAGCTATTCTTCTTCTCTGATCACAGAGGCACCAGGAACCATTGCTGCTGGGAGGAGGGAGCAGATGGCTCTTCAGCATGCACAGAGAAAGATGAGGATTGCTCATTATGGAAGATCAAATTCTGCAAATTTTGAAAGGGTTGCTCCAATTGATACAATGGAAGCCAAAGGTGGTGAAGAAGATCACAAAAGATGCAGCTTCATCACAGCTAATTCAGATCCCATATATGTTGCTTACCATGATCAAGAATGGGGAGTTCCTGTTCATGATGACAAGATGTTATTTGAACTGCTTGTTCTAAGTGGAGCTCAAGTTGGATCAGACTGGACTTCAATTTTAAAGAAACGCCAAGATTTCAGGGATGCCTTTTCTGGGTTTGATGCAGAAGCTGTGGCCAATCTCACTGACAAGCAAATGATTTCAATTTGTTCAGAATATGGCATTGATATAAGCAGAGTCAGAGGAGTAGTAGACAACTCTAACAGAATTCTTGAGGTAAAGAGGGAATTTGGGTCCTTTCACAAATACATATGGGGGTTTGTGAATCACAAGCCAATCTCCCCTCAATACAAGCAAGGCTACAAAATCCCAGTGAAGACATCAAAATCAGAGAGCATAAGCAAGGACATGGTTAGGAGGGGGTTTAGGTTTGTTGGTCCAACAGTTGTTCATTCCTTCATGCAAGCCTCAGGCCTCACAAATGACCATTTGACCACCTGTCACAGGCACCTTCAGTGCACCTTATTGGCAGCCCATCCTTTGGAACAAGCTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

411

Amino Acids

45.0

Weight (kDa)

9.77

Isoelectric Point (pI)

45.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 222 - 395 2.3e-65 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012861)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12710
fragaria_vesca FvH4_3g13890
malus_domestica MD05G1240400.v1.1 MD10G1218500.v1.1
prunus_persica Prupe.4G125700_v2.0.a1
pyrus_communis pycom05g21640 pycom10g18430
rosa_chinensis RchiOBHm_Chr5g0023071
rosa_laevigata RLG00000032698
rosa_multiflora Rmu_co8421393.1_g000001
rosa_roxburghii Rroxscaffold_1G00056250
rosa_rugosa Rorug05G0077100
rosa_samantha Rh5AG163200 Rh5BG162500 Rh5CG177300 Rh5DG163700
rosa_wichuraiana Rw5G014720 Rw5G014990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 338
AccB1I GGYRCC 3 cut(s) 338, 484, 1182
AccB7I CCANNNNNTGG 1 cut(s) 1216
AccI GTMKAC 2 cut(s) 424, 927
AciI CCGC 1 cut(s) 263
AclWI GGATC 2 cut(s) 668, 781
AcoI YGGCCR 1 cut(s) 849
AcsI RAATTY 4 cut(s) 574, 583, 942, 962
AcuI CTGAAG 3 cut(s) 65, 512, 1172
AfaI GTAC 2 cut(s) 208, 340
AfiI CCNNNNNNNGG 3 cut(s) 828, 1179, 1216
AgsI TTSAA 6 cut(s) 38, 304, 590, 743, 789, 879
AhdI GACNNNNNGTC 1 cut(s) 1173
AhlI ACTAGT 1 cut(s) 191
AjnI CCWGG 2 cut(s) 331, 487
AloI GAACNNNNNNTCC 2 cut(s) 702, 734
AluBI AGCT 9 cut(s) 9, 350, 388, 459, 654, 665, 764, 845, 1226
AluI AGCT 9 cut(s) 9, 350, 388, 459, 654, 665, 764, 845, 1226
Alw21I GWGCWC 3 cut(s) 191, 766, 1196
Alw44I GTGCAC 1 cut(s) 1192
AlwI GGATC 2 cut(s) 668, 781
AlwNI CAGNNNCTG 1 cut(s) 845
AoxI GGCC 3 cut(s) 15, 849, 1147
ApaLI GTGCAC 1 cut(s) 1192
ApeKI GCWGC 4 cut(s) 6, 500, 651, 1205
ApoI RAATTY 4 cut(s) 574, 583, 942, 962
Asp718I GGTACC 1 cut(s) 338
AspLEI GCGC 1 cut(s) 120
AspS9I GGNCC 2 cut(s) 969, 1112
AsuHPI GGTGA 2 cut(s) 34, 641
AvaII GGWCC 2 cut(s) 969, 1112
AxyI CCTNAGG 1 cut(s) 1144
BaeGI GKGCMC 1 cut(s) 1196
BalI TGGCCA 1 cut(s) 851
BanI GGYRCC 3 cut(s) 338, 484, 1182
BanII GRGCYC 1 cut(s) 766
BarI GAAGNNNNNNTAC 4 cut(s) 199, 231, 415, 447
BbsI GAAGAC 1 cut(s) 1058
Bbv12I GWGCWC 3 cut(s) 191, 766, 1196
BbvI GCAGC 4 cut(s) 18, 487, 663, 1217
BccI CCATC 2 cut(s) 514, 1218
BciT130I CCWGG 2 cut(s) 333, 489
BclI TGATCA 2 cut(s) 474, 697
BcuI ACTAGT 1 cut(s) 191
BfaI CTAG 5 cut(s) 57, 105, 110, 192, 198
BfmI CTRYAG 1 cut(s) 1229
BisI GCNGC 4 cut(s) 7, 501, 652, 1206
BlsI GCNGC 4 cut(s) 8, 502, 653, 1207
Bme1390I CCNGG 2 cut(s) 333, 489
Bme18I GGWCC 2 cut(s) 969, 1112
BmeRI GACNNNNNGTC 1 cut(s) 1173
BmgT120I GGNCC 2 cut(s) 969, 1112
BmiI GGNNCC 5 cut(s) 340, 486, 493, 970, 1184
BmrFI CCNGG 2 cut(s) 333, 489
BmrI ACTGGG 1 cut(s) 1040
BmsI GCATC 3 cut(s) 638, 808, 826
BmuI ACTGGG 1 cut(s) 1040
BpiI GAAGAC 1 cut(s) 1058
BplI GAGNNNNNCTC 2 cut(s) 541, 573
BpmI CTGGAG 2 cut(s) 292, 315
Bpu10I CCTNAGC 1 cut(s) 351
BpuEI CTTGAG 3 cut(s) 113, 750, 968
BsaBI GATNNNNATC 1 cut(s) 38
BsaJI CCNNGG 4 cut(s) 18, 27, 210, 418
BsaXI ACNNNNNCTCC 4 cut(s) 702, 732, 753, 783
Bsc4I CCNNNNNNNGG 3 cut(s) 828, 1179, 1216
Bse118I RCCGGY 1 cut(s) 114
Bse1I ACTGG 5 cut(s) 74, 233, 309, 785, 1046
Bse21I CCTNAGG 1 cut(s) 1144
Bse3DI GCAATG 2 cut(s) 292, 495
Bse8I GATNNNNATC 1 cut(s) 38
BseBI CCWGG 2 cut(s) 333, 489
BseDI CCNNGG 4 cut(s) 18, 27, 210, 418
BseGI GGATG 2 cut(s) 823, 1210
BseJI GATNNNNATC 1 cut(s) 38
BseLI CCNNNNNNNGG 3 cut(s) 828, 1179, 1216
BseMI GCAATG 2 cut(s) 292, 495
BseMII CTCAG 2 cut(s) 306, 1158
BseNI ACTGG 5 cut(s) 74, 233, 309, 785, 1046
BseRI GAGGAG 2 cut(s) 523, 933
BseSI GKGCMC 1 cut(s) 1196
BseXI GCAGC 4 cut(s) 18, 487, 663, 1217
BseYI CCCAGC 1 cut(s) 503
BsgI GTGCAG 1 cut(s) 25
BshFI GGCC 3 cut(s) 17, 851, 1149
BshNI GGYRCC 3 cut(s) 338, 484, 1182
BsiHKAI GWGCWC 3 cut(s) 191, 766, 1196
BsiSI CCGG 1 cut(s) 115
BslI CCNNNNNNNGG 3 cut(s) 828, 1179, 1216
BsnI GGCC 3 cut(s) 17, 851, 1149
Bsp1286I GDGCHC 3 cut(s) 191, 766, 1196
Bsp143I GATC 7 cut(s) 278, 474, 569, 637, 673, 697, 773
Bsp19I CCATGG 2 cut(s) 27, 418
BspACI CCGC 1 cut(s) 263
BspANI GGCC 3 cut(s) 17, 851, 1149
BspCNI CTCAG 2 cut(s) 307, 1157
BspHI TCATGA 2 cut(s) 412, 721
BspLI GGNNCC 5 cut(s) 340, 486, 493, 970, 1184
BspPI GGATC 2 cut(s) 668, 781
BspQI GCTCTTC 1 cut(s) 531
BspT107I GGYRCC 3 cut(s) 338, 484, 1182
BsrDI GCAATG 2 cut(s) 292, 495
BsrFI RCCGGY 1 cut(s) 114
BsrI ACTGG 5 cut(s) 74, 233, 309, 785, 1046
BssAI RCCGGY 1 cut(s) 114
BssECI CCNNGG 4 cut(s) 18, 27, 210, 418
BssMI GATC 7 cut(s) 278, 474, 569, 637, 673, 697, 773
BssT1I CCWWGG 4 cut(s) 18, 27, 210, 418
Bst2UI CCWGG 2 cut(s) 333, 489
Bst4CI ACNGT 1 cut(s) 1120
Bst6I CTCTTC 1 cut(s) 531
BstC8I GCNNGC 3 cut(s) 267, 534, 1140
BstDEI CTNAG 5 cut(s) 12, 315, 351, 755, 1144
BstDSI CCRYGG 2 cut(s) 27, 418
BstEII GGTNACC 1 cut(s) 22
BstENI CCTNNNNNAGG 1 cut(s) 1177
BstF5I GGATG 2 cut(s) 823, 1210
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 7 cut(s) 281, 477, 572, 640, 676, 700, 776
BstMBI GATC 7 cut(s) 278, 474, 569, 637, 673, 697, 773
BstMWI GCNNNNNNNGC 1 cut(s) 407
BstNI CCWGG 2 cut(s) 333, 489
BstNSI RCATGY 1 cut(s) 536
BstPI GGTNACC 1 cut(s) 22
BstSCI CCNGG 2 cut(s) 331, 487
BstSFI CTRYAG 1 cut(s) 1229
BstSLI GKGCMC 1 cut(s) 1196
BstV1I GCAGC 4 cut(s) 18, 487, 663, 1217
BstV2I GAAGAC 1 cut(s) 1058
BstX2I RGATCY 1 cut(s) 673
BstYI RGATCY 1 cut(s) 673
Bsu36I CCTNAGG 1 cut(s) 1144
BsuRI GGCC 3 cut(s) 17, 851, 1149
BtgI CCRYGG 2 cut(s) 27, 418
BtsCI GGATG 2 cut(s) 823, 1210
BtsIMutI CAGTG 3 cut(s) 858, 1053, 1196
Cac8I GCNNGC 3 cut(s) 267, 534, 1140
CaiI CAGNNNCTG 1 cut(s) 845
CciI TCATGA 2 cut(s) 412, 721
CfoI GCGC 1 cut(s) 120
Cfr10I RCCGGY 1 cut(s) 114
Cfr13I GGNCC 2 cut(s) 969, 1112
Csp6I GTAC 2 cut(s) 207, 339
CviAII CATG 8 cut(s) 28, 413, 419, 533, 695, 722, 1084, 1135
CviQI GTAC 2 cut(s) 207, 339
DdeI CTNAG 5 cut(s) 12, 315, 351, 755, 1144
DpnI GATC 7 cut(s) 280, 476, 571, 639, 675, 699, 775
DpnII GATC 7 cut(s) 278, 474, 569, 637, 673, 697, 773
DraI TTTAAA 1 cut(s) 795
DriI GACNNNNNGTC 1 cut(s) 1173
EaeI YGGCCR 1 cut(s) 849
Eam1104I CTCTTC 1 cut(s) 531
Eam1105I GACNNNNNGTC 1 cut(s) 1173
EarI CTCTTC 1 cut(s) 531
Ecl136II GAGCTC 1 cut(s) 764
Eco130I CCWWGG 4 cut(s) 18, 27, 210, 418
Eco147I AGGCCT 1 cut(s) 1149
Eco24I GRGCYC 1 cut(s) 766
Eco47I GGWCC 2 cut(s) 969, 1112
Eco53kI GAGCTC 1 cut(s) 764
Eco57I CTGAAG 3 cut(s) 65, 512, 1172
Eco81I CCTNAGG 1 cut(s) 1144
Eco91I GGTNACC 1 cut(s) 22
EcoICRI GAGCTC 1 cut(s) 764
EcoNI CCTNNNNNAGG 1 cut(s) 1177
EcoO109I RGGNCCY 1 cut(s) 969
EcoO65I GGTNACC 1 cut(s) 22
EcoRI GAATTC 1 cut(s) 942
EcoRII CCWGG 2 cut(s) 331, 487
EcoT14I CCWWGG 4 cut(s) 18, 27, 210, 418
EcoT38I GRGCYC 1 cut(s) 766
ErhI CCWWGG 4 cut(s) 18, 27, 210, 418
FaeI CATG 8 cut(s) 31, 416, 422, 536, 698, 725, 1087, 1138
FatI CATG 8 cut(s) 27, 412, 418, 532, 694, 721, 1083, 1134
FauNDI CATATG 1 cut(s) 986
FbaI TGATCA 2 cut(s) 474, 697
FblI GTMKAC 2 cut(s) 424, 927
Fnu4HI GCNGC 4 cut(s) 7, 501, 652, 1206
FokI GGATG 2 cut(s) 830, 1197
FriOI GRGCYC 1 cut(s) 766
Fsp4HI GCNGC 4 cut(s) 7, 501, 652, 1206
FspBI CTAG 5 cut(s) 57, 105, 110, 192, 198
GlaI GCGC 1 cut(s) 119
GluI GCNGC 4 cut(s) 7, 501, 652, 1206
GsaI CCCAGC 1 cut(s) 507
GsuI CTGGAG 2 cut(s) 292, 315
HaeIII GGCC 3 cut(s) 17, 851, 1149
HapII CCGG 1 cut(s) 115
HhaI GCGC 1 cut(s) 120
Hin1II CATG 8 cut(s) 31, 416, 422, 536, 698, 725, 1087, 1138
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HindIII AAGCTT 1 cut(s) 386
HinfI GANTC 4 cut(s) 60, 328, 912, 1000
HpaII CCGG 1 cut(s) 115
HphI GGTGA 2 cut(s) 34, 641
Hpy166II GTNNAC 3 cut(s) 425, 928, 1194
Hpy188I TCNGA 7 cut(s) 316, 474, 673, 778, 889, 917, 1066
Hpy188III TCNNGA 6 cut(s) 57, 130, 413, 701, 722, 947
Hpy8I GTNNAC 3 cut(s) 425, 928, 1194
HpyAV CCTTC 2 cut(s) 1141, 1196
HpyCH4III ACNGT 1 cut(s) 1120
HpyCH4V TGCA 7 cut(s) 6, 536, 581, 651, 839, 1138, 1194
HpyF10VI GCNNNNNNNGC 1 cut(s) 407
HpyF3I CTNAG 5 cut(s) 12, 315, 351, 755, 1144
Hsp92II CATG 8 cut(s) 31, 416, 422, 536, 698, 725, 1087, 1138
HspAI GCGC 1 cut(s) 118
KpnI GGTACC 1 cut(s) 342
Ksp22I TGATCA 2 cut(s) 474, 697
Kzo9I GATC 7 cut(s) 278, 474, 569, 637, 673, 697, 773
LguI GCTCTTC 1 cut(s) 531
LmnI GCTCC 3 cut(s) 514, 604, 761
Lsp1109I GCAGC 4 cut(s) 18, 487, 663, 1217
LweI GCATC 3 cut(s) 638, 808, 826
MaeI CTAG 5 cut(s) 57, 105, 110, 192, 198
MaeIII GTNAC 4 cut(s) 22, 92, 325, 1175
MalI GATC 7 cut(s) 280, 476, 571, 639, 675, 699, 775
MboI GATC 7 cut(s) 278, 474, 569, 637, 673, 697, 773
MfeI CAATTG 1 cut(s) 603
MflI RGATCY 1 cut(s) 673
MhlI GDGCHC 3 cut(s) 191, 766, 1196
MlsI TGGCCA 1 cut(s) 851
MluNI TGGCCA 1 cut(s) 851
MlyI GAGTC 2 cut(s) 322, 921
MmeI TCCRAC 2 cut(s) 751, 1139
Mox20I TGGCCA 1 cut(s) 851
MscI TGGCCA 1 cut(s) 851
MseI TTAA 2 cut(s) 384, 794
MslI CAYNNNNRTG 1 cut(s) 417
Msp20I TGGCCA 1 cut(s) 851
MspI CCGG 1 cut(s) 115
MspR9I CCNGG 2 cut(s) 333, 489
MunI CAATTG 1 cut(s) 603
MvaI CCWGG 2 cut(s) 333, 489
MwoI GCNNNNNNNGC 1 cut(s) 407
NcoI CCATGG 2 cut(s) 27, 418
NdeI CATATG 1 cut(s) 986
NdeII GATC 7 cut(s) 278, 474, 569, 637, 673, 697, 773
NlaIII CATG 8 cut(s) 31, 416, 422, 536, 698, 725, 1087, 1138
NlaIV GGNNCC 5 cut(s) 340, 486, 493, 970, 1184
NmuCI GTSAC 3 cut(s) 22, 325, 1175
NspI RCATGY 1 cut(s) 536
PaeI GCATGC 1 cut(s) 536
PagI TCATGA 2 cut(s) 412, 721
PceI AGGCCT 1 cut(s) 1149
PciSI GCTCTTC 1 cut(s) 531
PfeI GAWTC 2 cut(s) 60, 1000
PflMI CCANNNNNTGG 1 cut(s) 1216
PfoI TCCNGGA 1 cut(s) 331
PkrI GCNGC 4 cut(s) 8, 502, 653, 1207
PleI GAGTC 2 cut(s) 322, 920
PpsI GAGTC 2 cut(s) 322, 920
PpuMI RGGWCCY 1 cut(s) 969
Psp124BI GAGCTC 1 cut(s) 766
Psp5II RGGWCCY 1 cut(s) 969
Psp6I CCWGG 2 cut(s) 331, 487
PspEI GGTNACC 1 cut(s) 22
PspFI CCCAGC 1 cut(s) 503
PspGI CCWGG 2 cut(s) 331, 487
PspN4I GGNNCC 5 cut(s) 340, 486, 493, 970, 1184
PspPI GGNCC 2 cut(s) 969, 1112
PspPPI RGGWCCY 1 cut(s) 969
PstNI CAGNNNCTG 1 cut(s) 845
PsuI RGATCY 1 cut(s) 673
RsaI GTAC 2 cut(s) 208, 340
RsaNI GTAC 2 cut(s) 207, 339
RseI CAYNNNNRTG 1 cut(s) 417
SacI GAGCTC 1 cut(s) 766
SapI GCTCTTC 1 cut(s) 531
SaqAI TTAA 2 cut(s) 384, 794
SatI GCNGC 4 cut(s) 7, 501, 652, 1206
Sau3AI GATC 7 cut(s) 278, 474, 569, 637, 673, 697, 773
Sau96I GGNCC 2 cut(s) 969, 1112
SchI GAGTC 2 cut(s) 322, 921
ScrFI CCNGG 2 cut(s) 333, 489
SduI GDGCHC 3 cut(s) 191, 766, 1196
SfaNI GCATC 3 cut(s) 638, 808, 826
SfcI CTRYAG 1 cut(s) 1229
SinI GGWCC 2 cut(s) 969, 1112
SmiMI CAYNNNNRTG 1 cut(s) 417
SmlI CTYRAG 3 cut(s) 128, 765, 947
SmoI CTYRAG 3 cut(s) 128, 765, 947
SpeI ACTAGT 1 cut(s) 191
SphI GCATGC 1 cut(s) 536
SseBI AGGCCT 1 cut(s) 1149
SsiI CCGC 1 cut(s) 263
SspMI CTAG 5 cut(s) 57, 105, 110, 192, 198
SstI GAGCTC 1 cut(s) 766
StuI AGGCCT 1 cut(s) 1149
StyD4I CCNGG 2 cut(s) 331, 487
StyI CCWWGG 4 cut(s) 18, 27, 210, 418
TaaI ACNGT 1 cut(s) 1120
TaqI TCGA 1 cut(s) 440
TfiI GAWTC 2 cut(s) 60, 1000
Tru1I TTAA 2 cut(s) 384, 794
Tru9I TTAA 2 cut(s) 384, 794
TscAI CASTG 3 cut(s) 865, 1053, 1196
TseFI GTSAC 3 cut(s) 22, 325, 1175
TseI GCWGC 4 cut(s) 6, 500, 651, 1205
Tsp45I GTSAC 3 cut(s) 22, 325, 1175
TspDTI ATGAA 5 cut(s) 207, 646, 710, 1115, 1123
TspRI CASTG 3 cut(s) 865, 1053, 1196
Van91I CCANNNNNTGG 1 cut(s) 1216
VneI GTGCAC 1 cut(s) 1192
VpaK11BI GGWCC 2 cut(s) 969, 1112
XagI CCTNNNNNAGG 1 cut(s) 1177
XapI RAATTY 4 cut(s) 574, 583, 942, 962
XbaI TCTAGA 1 cut(s) 56
XceI RCATGY 1 cut(s) 536
XcmI CCANNNNNNNNNTGG 1 cut(s) 25
XmiI GTMKAC 2 cut(s) 424, 927
XspI CTAG 5 cut(s) 57, 105, 110, 192, 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.