Rw5G014720

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0012861
Physical Location & Seq
Reverse (-)
17672490 .. 17675576
3087 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G014720.1

Sequence Viewer

Length: 1233 bp
ATGTGCAGCTCTAAGGCCAAGGTGACCATGGGGATTGAAATCACACCCCTTGTTTCTAGAATCAATGGCAGACCAGTGCTTCAGCCTACCTGCAATCGAGTTCCTAGCCTAGACCGGCGCAATTCCCTTAAGAAACTGTCAACAGCACCACCACCTCTACCATTATCAACTTCTTCTACCAGCACTAGTCCTAGAATTAGTACCAAGGCTTCATTGACAACACCACCAATTTCTCCAAAGTCAAAATCTCCGAGGCCACCTGCGATAAAGAGATCAGGCAATGACCCTAATGGCTTGAACTCCAGTTCTGAGAAAGTTGTGACACCAGGAGGTACCACAAGAGCTAAGGTTTTGGAAAGGAAGAAGTCAAAGAGTTTTAAGCTTGGTGTTGGTGCTGATAATGCTCATGATCATGGTAGACTCTCTTCTGCTTCGATTGAGGCTTCGTTGAGCTACTCCTCTTCTCTGATCACCGAGGCACCAGGAACCATTGCAGCTGGGAGGAGGGAGCAGATGGCTCTTCAACATGCACAGAGAAAGATGAGGATTGCTCATTATGGAAGATCAAAGTCTGCAAATTTTGAAAGGGTTGCTCCAGTTGATACTATGGAAGCCAAAGCTGGTGAAGAAGATCACAAAAGATGCAGCTTCATCACGGCTAATTCAGATCCCATTTATGTTGCTTACCATGATCAAGAATGGGGAGTTCCTGTTCATGATGACAAGATGTTATTTGAACTGCTTGTTCTAAGTGGAGCTCAAGTTGGATCAGATTGGACTTCCATTTTAAAGAAACGCCAAGATTTCAGGGATGCATTTTCTGGGTTTGATGCTGAAGCTGTGGCCAGTCTCACTGACAAGCAAATGATTTCGATTTGTTCAGAATATGGCATTGATATAAGCAGAGTTAGAGGAGTAGTAGACAACTCTAACAGAATTCTTGAGGTAAAGAAGGAATTTGGGTCATTTCACAAATACATATGGGGGTTTGTGAATCACAAGCCAATTTCCCCTCAATACAAGCAAGGCTACAAAATCCCAGTGAAGACATCAAAATCAGAGAGCATAAGCAAAGACATGGTTAGGAGGGGGTTTAGGTTTGTTGGTCCAACAGTTGTTCATTCCTTCATGCAAGCCTCAGGCCTCACAAATGACCATTTGACCACCTGCCACAGGCACCTTCAGTGCACCTTACTAGCAGCCCATCCTACCACATTGGAACAAGCTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

410

Amino Acids

44.97

Weight (kDa)

9.78

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 220 - 393 1.7e-65 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012861)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12710
fragaria_vesca FvH4_3g13890
malus_domestica MD05G1240400.v1.1 MD10G1218500.v1.1
prunus_persica Prupe.4G125700_v2.0.a1
pyrus_communis pycom05g21640 pycom10g18430
rosa_chinensis RchiOBHm_Chr5g0023071
rosa_laevigata RLG00000032698
rosa_multiflora Rmu_co8421393.1_g000001
rosa_roxburghii Rroxscaffold_1G00056250
rosa_rugosa Rorug05G0077100
rosa_samantha Rh5AG163200 Rh5BG162500 Rh5CG177300 Rh5DG163700
rosa_wichuraiana Rw5G014720 Rw5G014990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 268, 1175
Acc36I ACCTGC 3 cut(s) 98, 268, 1175
Acc65I GGTACC 1 cut(s) 332
AccB1I GGYRCC 3 cut(s) 332, 478, 1176
AccI GTMKAC 2 cut(s) 418, 921
AclWI GGATC 2 cut(s) 662, 775
AcoI YGGCCR 1 cut(s) 843
AcsI RAATTY 3 cut(s) 577, 936, 956
AcuI CTGAAG 3 cut(s) 65, 855, 1166
AfaI GTAC 2 cut(s) 202, 334
AfiI CCNNNNNNNGG 1 cut(s) 1173
AflII CTTAAG 1 cut(s) 128
AgsI TTSAA 5 cut(s) 38, 298, 524, 584, 737
AhlI ACTAGT 1 cut(s) 185
AjnI CCWGG 2 cut(s) 325, 481
AloI GAACNNNNNNTCC 2 cut(s) 696, 728
Alw21I GWGCWC 2 cut(s) 760, 1190
Alw26I GTCTC 1 cut(s) 854
Alw44I GTGCAC 1 cut(s) 1186
AlwI GGATC 2 cut(s) 662, 775
AoxI GGCC 4 cut(s) 15, 254, 843, 1141
ApaLI GTGCAC 1 cut(s) 1186
ApeKI GCWGC 4 cut(s) 6, 494, 645, 1199
ApoI RAATTY 3 cut(s) 577, 936, 956
Asp718I GGTACC 1 cut(s) 332
AspLEI GCGC 1 cut(s) 120
AspS9I GGNCC 1 cut(s) 1106
AsuHPI GGTGA 3 cut(s) 34, 463, 635
AvaII GGWCC 1 cut(s) 1106
AxyI CCTNAGG 1 cut(s) 1138
BaeGI GKGCMC 1 cut(s) 1190
BalI TGGCCA 1 cut(s) 845
BanI GGYRCC 3 cut(s) 332, 478, 1176
BanII GRGCYC 1 cut(s) 760
BarI GAAGNNNNNNTAC 4 cut(s) 193, 225, 409, 441
BbsI GAAGAC 1 cut(s) 1052
Bbv12I GWGCWC 2 cut(s) 760, 1190
BbvI GCAGC 4 cut(s) 18, 506, 657, 1211
BccI CCATC 2 cut(s) 508, 1212
BceAI ACGGC 1 cut(s) 672
BciT130I CCWGG 2 cut(s) 327, 483
BclI TGATCA 3 cut(s) 409, 468, 691
BcoDI GTCTC 1 cut(s) 854
BcuI ACTAGT 1 cut(s) 185
BfaI CTAG 6 cut(s) 57, 105, 110, 186, 192, 1196
BfmI CTRYAG 1 cut(s) 1229
BfrI CTTAAG 1 cut(s) 128
BfuAI ACCTGC 3 cut(s) 98, 268, 1175
BisI GCNGC 4 cut(s) 7, 495, 646, 1200
BlsI GCNGC 4 cut(s) 8, 496, 647, 1201
Bme1390I CCNGG 2 cut(s) 327, 483
Bme18I GGWCC 1 cut(s) 1106
BmgT120I GGNCC 1 cut(s) 1106
BmiI GGNNCC 4 cut(s) 334, 480, 487, 1178
BmrFI CCNGG 2 cut(s) 327, 483
BmrI ACTGGG 1 cut(s) 1034
BmsI GCATC 3 cut(s) 632, 802, 820
BmuI ACTGGG 1 cut(s) 1034
BpiI GAAGAC 1 cut(s) 1052
BplI GAGNNNNNCTC 2 cut(s) 535, 567
BpmI CTGGAG 2 cut(s) 286, 579
Bpu10I CCTNAGC 1 cut(s) 345
BpuEI CTTGAG 2 cut(s) 744, 962
BsaBI GATNNNNATC 1 cut(s) 38
BsaJI CCNNGG 5 cut(s) 18, 27, 204, 251, 474
BsaXI ACNNNNNCTCC 4 cut(s) 696, 726, 747, 777
Bsc4I CCNNNNNNNGG 1 cut(s) 1173
Bse118I RCCGGY 1 cut(s) 114
Bse1I ACTGG 5 cut(s) 74, 303, 596, 846, 1040
Bse21I CCTNAGG 1 cut(s) 1138
Bse3DI GCAATG 2 cut(s) 286, 489
Bse8I GATNNNNATC 1 cut(s) 38
BseBI CCWGG 2 cut(s) 327, 483
BseDI CCNNGG 5 cut(s) 18, 27, 204, 251, 474
BseGI GGATG 2 cut(s) 817, 1204
BseJI GATNNNNATC 1 cut(s) 38
BseLI CCNNNNNNNGG 1 cut(s) 1173
BseMI GCAATG 2 cut(s) 286, 489
BseMII CTCAG 2 cut(s) 300, 1152
BseNI ACTGG 5 cut(s) 74, 303, 596, 846, 1040
BseRI GAGGAG 3 cut(s) 448, 517, 927
BseSI GKGCMC 1 cut(s) 1190
BseXI GCAGC 4 cut(s) 18, 506, 657, 1211
BseYI CCCAGC 1 cut(s) 497
BsgI GTGCAG 1 cut(s) 25
BshFI GGCC 4 cut(s) 17, 256, 845, 1143
BshNI GGYRCC 3 cut(s) 332, 478, 1176
BsiHKAI GWGCWC 2 cut(s) 760, 1190
BsiSI CCGG 1 cut(s) 115
BslI CCNNNNNNNGG 1 cut(s) 1173
BsmAI GTCTC 1 cut(s) 854
BsnI GGCC 4 cut(s) 17, 256, 845, 1143
Bsp1286I GDGCHC 2 cut(s) 760, 1190
Bsp143I GATC 8 cut(s) 272, 409, 468, 563, 631, 667, 691, 767
Bsp19I CCATGG 1 cut(s) 27
BspANI GGCC 4 cut(s) 17, 256, 845, 1143
BspCNI CTCAG 2 cut(s) 301, 1151
BspHI TCATGA 2 cut(s) 406, 715
BspLI GGNNCC 4 cut(s) 334, 480, 487, 1178
BspMI ACCTGC 3 cut(s) 98, 268, 1175
BspPI GGATC 2 cut(s) 662, 775
BspQI GCTCTTC 1 cut(s) 525
BspT107I GGYRCC 3 cut(s) 332, 478, 1176
BspTI CTTAAG 1 cut(s) 128
BsrDI GCAATG 2 cut(s) 286, 489
BsrFI RCCGGY 1 cut(s) 114
BsrI ACTGG 5 cut(s) 74, 303, 596, 846, 1040
BssAI RCCGGY 1 cut(s) 114
BssECI CCNNGG 5 cut(s) 18, 27, 204, 251, 474
BssMI GATC 8 cut(s) 272, 409, 468, 563, 631, 667, 691, 767
BssT1I CCWWGG 3 cut(s) 18, 27, 204
Bst2UI CCWGG 2 cut(s) 327, 483
Bst4CI ACNGT 2 cut(s) 138, 1114
Bst6I CTCTTC 3 cut(s) 430, 466, 525
BstAFI CTTAAG 1 cut(s) 128
BstC8I GCNNGC 1 cut(s) 1134
BstDEI CTNAG 5 cut(s) 12, 309, 345, 749, 1138
BstDSI CCRYGG 1 cut(s) 27
BstEII GGTNACC 1 cut(s) 22
BstENI CCTNNNNNAGG 1 cut(s) 1171
BstF5I GGATG 2 cut(s) 817, 1204
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 8 cut(s) 275, 412, 471, 566, 634, 670, 694, 770
BstMAI GTCTC 1 cut(s) 854
BstMBI GATC 8 cut(s) 272, 409, 468, 563, 631, 667, 691, 767
BstMWI GCNNNNNNNGC 1 cut(s) 401
BstNI CCWGG 2 cut(s) 327, 483
BstNSI RCATGY 1 cut(s) 530
BstPI GGTNACC 1 cut(s) 22
BstSCI CCNGG 2 cut(s) 325, 481
BstSFI CTRYAG 1 cut(s) 1229
BstSLI GKGCMC 1 cut(s) 1190
BstV1I GCAGC 4 cut(s) 18, 506, 657, 1211
BstV2I GAAGAC 1 cut(s) 1052
BstX2I RGATCY 1 cut(s) 667
BstYI RGATCY 1 cut(s) 667
Bsu36I CCTNAGG 1 cut(s) 1138
BsuRI GGCC 4 cut(s) 17, 256, 845, 1143
BtgI CCRYGG 1 cut(s) 27
BtsCI GGATG 2 cut(s) 817, 1204
BtsIMutI CAGTG 4 cut(s) 81, 852, 1047, 1190
BveI ACCTGC 3 cut(s) 98, 268, 1175
Cac8I GCNNGC 1 cut(s) 1134
CciI TCATGA 2 cut(s) 406, 715
CfoI GCGC 1 cut(s) 120
Cfr10I RCCGGY 1 cut(s) 114
Cfr13I GGNCC 1 cut(s) 1106
Csp6I GTAC 2 cut(s) 201, 333
CviAII CATG 8 cut(s) 28, 407, 413, 527, 689, 716, 1078, 1129
CviQI GTAC 2 cut(s) 201, 333
DdeI CTNAG 5 cut(s) 12, 309, 345, 749, 1138
DpnI GATC 8 cut(s) 274, 411, 470, 565, 633, 669, 693, 769
DpnII GATC 8 cut(s) 272, 409, 468, 563, 631, 667, 691, 767
DraI TTTAAA 1 cut(s) 789
EaeI YGGCCR 1 cut(s) 843
Eam1104I CTCTTC 3 cut(s) 430, 466, 525
EarI CTCTTC 3 cut(s) 430, 466, 525
Ecl136II GAGCTC 1 cut(s) 758
Eco130I CCWWGG 3 cut(s) 18, 27, 204
Eco147I AGGCCT 1 cut(s) 1143
Eco24I GRGCYC 1 cut(s) 760
Eco47I GGWCC 1 cut(s) 1106
Eco53kI GAGCTC 1 cut(s) 758
Eco57I CTGAAG 3 cut(s) 65, 855, 1166
Eco81I CCTNAGG 1 cut(s) 1138
Eco91I GGTNACC 1 cut(s) 22
EcoICRI GAGCTC 1 cut(s) 758
EcoNI CCTNNNNNAGG 1 cut(s) 1171
EcoO65I GGTNACC 1 cut(s) 22
EcoRI GAATTC 1 cut(s) 936
EcoRII CCWGG 2 cut(s) 325, 481
EcoT14I CCWWGG 3 cut(s) 18, 27, 204
EcoT22I ATGCAT 1 cut(s) 817
EcoT38I GRGCYC 1 cut(s) 760
ErhI CCWWGG 3 cut(s) 18, 27, 204
FaeI CATG 8 cut(s) 31, 410, 416, 530, 692, 719, 1081, 1132
FatI CATG 8 cut(s) 27, 406, 412, 526, 688, 715, 1077, 1128
FauNDI CATATG 1 cut(s) 980
FbaI TGATCA 3 cut(s) 409, 468, 691
FblI GTMKAC 2 cut(s) 418, 921
Fnu4HI GCNGC 4 cut(s) 7, 495, 646, 1200
FokI GGATG 2 cut(s) 824, 1191
FriOI GRGCYC 1 cut(s) 760
Fsp4HI GCNGC 4 cut(s) 7, 495, 646, 1200
FspBI CTAG 6 cut(s) 57, 105, 110, 186, 192, 1196
GlaI GCGC 1 cut(s) 119
GluI GCNGC 4 cut(s) 7, 495, 646, 1200
GsaI CCCAGC 1 cut(s) 501
GsuI CTGGAG 2 cut(s) 286, 579
HaeIII GGCC 4 cut(s) 17, 256, 845, 1143
HapII CCGG 1 cut(s) 115
HhaI GCGC 1 cut(s) 120
Hin1II CATG 8 cut(s) 31, 410, 416, 530, 692, 719, 1081, 1132
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HincII GTYRAC 1 cut(s) 141
HindII GTYRAC 1 cut(s) 141
HindIII AAGCTT 1 cut(s) 380
HinfI GANTC 3 cut(s) 60, 420, 994
HpaII CCGG 1 cut(s) 115
HphI GGTGA 3 cut(s) 34, 463, 635
Hpy166II GTNNAC 4 cut(s) 141, 419, 922, 1188
Hpy188I TCNGA 7 cut(s) 252, 310, 468, 667, 772, 883, 1060
Hpy188III TCNNGA 5 cut(s) 57, 407, 695, 716, 941
Hpy8I GTNNAC 4 cut(s) 141, 419, 922, 1188
HpyAV CCTTC 3 cut(s) 946, 1135, 1190
HpyCH4III ACNGT 2 cut(s) 138, 1114
HpyCH4V TGCA 9 cut(s) 6, 93, 494, 530, 575, 645, 815, 1132, 1188
HpyF10VI GCNNNNNNNGC 1 cut(s) 401
HpyF3I CTNAG 5 cut(s) 12, 309, 345, 749, 1138
Hsp92II CATG 8 cut(s) 31, 410, 416, 530, 692, 719, 1081, 1132
HspAI GCGC 1 cut(s) 118
KpnI GGTACC 1 cut(s) 336
Ksp22I TGATCA 3 cut(s) 409, 468, 691
Kzo9I GATC 8 cut(s) 272, 409, 468, 563, 631, 667, 691, 767
LguI GCTCTTC 1 cut(s) 525
LmnI GCTCC 3 cut(s) 508, 598, 755
Lsp1109I GCAGC 4 cut(s) 18, 506, 657, 1211
LweI GCATC 3 cut(s) 632, 802, 820
MaeI CTAG 6 cut(s) 57, 105, 110, 186, 192, 1196
MaeIII GTNAC 2 cut(s) 22, 319
MalI GATC 8 cut(s) 274, 411, 470, 565, 633, 669, 693, 769
MboI GATC 8 cut(s) 272, 409, 468, 563, 631, 667, 691, 767
MboII GAAGA 9 cut(s) 165, 373, 417, 453, 512, 573, 638, 641, 1057
MflI RGATCY 1 cut(s) 667
MhlI GDGCHC 2 cut(s) 760, 1190
MlsI TGGCCA 1 cut(s) 845
MluCI AATT 8 cut(s) 121, 195, 228, 577, 661, 936, 956, 1005
MluNI TGGCCA 1 cut(s) 845
MlyI GAGTC 1 cut(s) 414
MmeI TCCRAC 2 cut(s) 745, 1133
Mox20I TGGCCA 1 cut(s) 845
Mph1103I ATGCAT 1 cut(s) 817
MscI TGGCCA 1 cut(s) 845
MseI TTAA 3 cut(s) 129, 378, 788
MslI CAYNNNNRTG 1 cut(s) 411
Msp20I TGGCCA 1 cut(s) 845
MspA1I CMGCKG 1 cut(s) 497
MspCI CTTAAG 1 cut(s) 128
MspI CCGG 1 cut(s) 115
MspR9I CCNGG 2 cut(s) 327, 483
MvaI CCWGG 2 cut(s) 327, 483
MwoI GCNNNNNNNGC 1 cut(s) 401
NcoI CCATGG 1 cut(s) 27
NdeI CATATG 1 cut(s) 980
NdeII GATC 8 cut(s) 272, 409, 468, 563, 631, 667, 691, 767
NlaIII CATG 8 cut(s) 31, 410, 416, 530, 692, 719, 1081, 1132
NlaIV GGNNCC 4 cut(s) 334, 480, 487, 1178
NmuCI GTSAC 2 cut(s) 22, 319
NsiI ATGCAT 1 cut(s) 817
NspI RCATGY 1 cut(s) 530
PagI TCATGA 2 cut(s) 406, 715
PaqCI CACCTGC 2 cut(s) 268, 1175
PceI AGGCCT 1 cut(s) 1143
PciSI GCTCTTC 1 cut(s) 525
PfeI GAWTC 2 cut(s) 60, 994
PkrI GCNGC 4 cut(s) 8, 496, 647, 1201
PleI GAGTC 1 cut(s) 414
PpsI GAGTC 1 cut(s) 414
Psp124BI GAGCTC 1 cut(s) 760
Psp6I CCWGG 2 cut(s) 325, 481
PspEI GGTNACC 1 cut(s) 22
PspFI CCCAGC 1 cut(s) 497
PspGI CCWGG 2 cut(s) 325, 481
PspN4I GGNNCC 4 cut(s) 334, 480, 487, 1178
PspPI GGNCC 1 cut(s) 1106
PsuI RGATCY 1 cut(s) 667
PvuII CAGCTG 1 cut(s) 497
RsaI GTAC 2 cut(s) 202, 334
RsaNI GTAC 2 cut(s) 201, 333
RseI CAYNNNNRTG 1 cut(s) 411
SacI GAGCTC 1 cut(s) 760
SapI GCTCTTC 1 cut(s) 525
SaqAI TTAA 3 cut(s) 129, 378, 788
SatI GCNGC 4 cut(s) 7, 495, 646, 1200
Sau3AI GATC 8 cut(s) 272, 409, 468, 563, 631, 667, 691, 767
Sau96I GGNCC 1 cut(s) 1106
SchI GAGTC 1 cut(s) 414
ScrFI CCNGG 2 cut(s) 327, 483
SduI GDGCHC 2 cut(s) 760, 1190
SfaNI GCATC 3 cut(s) 632, 802, 820
SfcI CTRYAG 1 cut(s) 1229
SinI GGWCC 1 cut(s) 1106
SmiMI CAYNNNNRTG 1 cut(s) 411
SmlI CTYRAG 3 cut(s) 128, 759, 941
SmoI CTYRAG 3 cut(s) 128, 759, 941
SpeI ACTAGT 1 cut(s) 185
Sse9I AATT 8 cut(s) 121, 195, 228, 577, 661, 936, 956, 1005
SseBI AGGCCT 1 cut(s) 1143
SspMI CTAG 6 cut(s) 57, 105, 110, 186, 192, 1196
SstI GAGCTC 1 cut(s) 760
StuI AGGCCT 1 cut(s) 1143
StyD4I CCNGG 2 cut(s) 325, 481
StyI CCWWGG 3 cut(s) 18, 27, 204
TaaI ACNGT 2 cut(s) 138, 1114
TaqI TCGA 3 cut(s) 97, 434, 872
TasI AATT 8 cut(s) 121, 195, 228, 577, 661, 936, 956, 1005
TfiI GAWTC 2 cut(s) 60, 994
Tru1I TTAA 3 cut(s) 129, 378, 788
Tru9I TTAA 3 cut(s) 129, 378, 788
TscAI CASTG 4 cut(s) 81, 859, 1047, 1190
TseFI GTSAC 2 cut(s) 22, 319
TseI GCWGC 4 cut(s) 6, 494, 645, 1199
Tsp45I GTSAC 2 cut(s) 22, 319
TspDTI ATGAA 5 cut(s) 201, 640, 704, 1109, 1117
TspRI CASTG 4 cut(s) 81, 859, 1047, 1190
Vha464I CTTAAG 1 cut(s) 128
VneI GTGCAC 1 cut(s) 1186
VpaK11BI GGWCC 1 cut(s) 1106
XagI CCTNNNNNAGG 1 cut(s) 1171
XapI RAATTY 3 cut(s) 577, 936, 956
XbaI TCTAGA 1 cut(s) 56
XceI RCATGY 1 cut(s) 530
XcmI CCANNNNNNNNNTGG 1 cut(s) 25
XmiI GTMKAC 2 cut(s) 418, 921
XspI CTAG 6 cut(s) 57, 105, 110, 186, 192, 1196
Zsp2I ATGCAT 1 cut(s) 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.