Rmu_co8421393.1_g000001

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8421393.1
OG0012861
Physical Location & Seq
Forward (+)
239 .. 1414
1176 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8421393.1_g000001.1.cds

Sequence Viewer

Length: 919 bp
atgtgcagctctaaggccaaggtgaccatggggattgaaatcacaccccttgtttctagaatcaatggcagacaagtgcttcagcctacctgcaatcgagttcctagcctagaccggcgcaattcccttaagaaactgtcaacagcaccaccacctctaccattatcaacttcttctaccagcactagtcctagaattagtaccaaggcttcattgacaacaccaccaatttctccaaagtcaaaatctccgaggccacctgcgataaagagatcaggcaatgaccctaatggcttgaactccagttctgagaaagttgtgacaccaggaggtaccacaagagctaaggttttggaaaggaagaagtcaaagagttttaagcttggtgttggtgctgataatgctcatgatcatggtagactctcttctgcttcgattgaggcttcgttgagctactcctcttctctgatcaccgaggcaccaggaaccattgcagctgggaggagggagcagatggctcttcaacatgcacagagaaagatgaggattgctcattatggaagatcaaagtctgcaaattttgaaagggttgctccagttgatactatggaagccaaagctggtgaagaagatcacaaaagatgcagcttcatcacggctaattcagatcccatttatgttgcttaccatgatcaagaatggggagttcctgttcatgatgacaagatgttatttgaactgcttgttctaagtggagctcaagttggatcagattggacttccattttaaagaaacgccaagatttcagggatgcattttctgggtttgatgctgaagctgtggccagtctcactgacaagcaaatgatttcgatttgttcagaatatggcattgatataagcagagttagaggagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

306

Amino Acids

33.15

Weight (kDa)

9.74

Isoelectric Point (pI)

45.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012861)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12710
fragaria_vesca FvH4_3g13890
malus_domestica MD05G1240400.v1.1 MD10G1218500.v1.1
prunus_persica Prupe.4G125700_v2.0.a1
pyrus_communis pycom05g21640 pycom10g18430
rosa_chinensis RchiOBHm_Chr5g0023071
rosa_laevigata RLG00000032698
rosa_multiflora Rmu_co8421393.1_g000001
rosa_roxburghii Rroxscaffold_1G00056250
rosa_rugosa Rorug05G0077100
rosa_samantha Rh5AG163200 Rh5BG162500 Rh5CG177300 Rh5DG163700
rosa_wichuraiana Rw5G014720 Rw5G014990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 268
Acc36I ACCTGC 2 cut(s) 98, 268
Acc65I GGTACC 1 cut(s) 332
AccB1I GGYRCC 2 cut(s) 332, 478
AccI GTMKAC 1 cut(s) 418
AclWI GGATC 2 cut(s) 662, 775
AcoI YGGCCR 1 cut(s) 843
AcsI RAATTY 1 cut(s) 577
AcuI CTGAAG 2 cut(s) 65, 855
AfaI GTAC 2 cut(s) 202, 334
AflII CTTAAG 1 cut(s) 128
AgsI TTSAA 5 cut(s) 38, 298, 524, 584, 737
AhlI ACTAGT 1 cut(s) 185
AjnI CCWGG 2 cut(s) 325, 481
AloI GAACNNNNNNTCC 2 cut(s) 696, 728
AluBI AGCT 9 cut(s) 9, 344, 382, 453, 497, 620, 648, 758, 839
AluI AGCT 9 cut(s) 9, 344, 382, 453, 497, 620, 648, 758, 839
Alw21I GWGCWC 1 cut(s) 760
Alw26I GTCTC 1 cut(s) 854
AlwI GGATC 2 cut(s) 662, 775
AoxI GGCC 3 cut(s) 15, 254, 843
ApeKI GCWGC 3 cut(s) 6, 494, 645
ApoI RAATTY 1 cut(s) 577
Asp718I GGTACC 1 cut(s) 332
AspLEI GCGC 1 cut(s) 120
AsuHPI GGTGA 3 cut(s) 34, 463, 635
BalI TGGCCA 1 cut(s) 845
BanI GGYRCC 2 cut(s) 332, 478
BanII GRGCYC 1 cut(s) 760
BarI GAAGNNNNNNTAC 4 cut(s) 193, 225, 409, 441
Bbv12I GWGCWC 1 cut(s) 760
BbvI GCAGC 3 cut(s) 18, 506, 657
BccI CCATC 1 cut(s) 508
BceAI ACGGC 1 cut(s) 672
BciT130I CCWGG 2 cut(s) 327, 483
BclI TGATCA 3 cut(s) 409, 468, 691
BcoDI GTCTC 1 cut(s) 854
BcuI ACTAGT 1 cut(s) 185
BfaI CTAG 5 cut(s) 57, 105, 110, 186, 192
BfrI CTTAAG 1 cut(s) 128
BfuAI ACCTGC 2 cut(s) 98, 268
BisI GCNGC 3 cut(s) 7, 495, 646
BlsI GCNGC 3 cut(s) 8, 496, 647
Bme1390I CCNGG 2 cut(s) 327, 483
BmiI GGNNCC 3 cut(s) 334, 480, 487
BmrFI CCNGG 2 cut(s) 327, 483
BmsI GCATC 3 cut(s) 632, 802, 820
BplI GAGNNNNNCTC 2 cut(s) 535, 567
BpmI CTGGAG 2 cut(s) 286, 579
Bpu10I CCTNAGC 1 cut(s) 345
BpuEI CTTGAG 1 cut(s) 744
BsaBI GATNNNNATC 1 cut(s) 38
BsaJI CCNNGG 5 cut(s) 18, 27, 204, 251, 474
BsaXI ACNNNNNCTCC 4 cut(s) 696, 726, 747, 777
Bse118I RCCGGY 1 cut(s) 114
Bse1I ACTGG 3 cut(s) 303, 596, 846
Bse3DI GCAATG 2 cut(s) 286, 489
Bse8I GATNNNNATC 1 cut(s) 38
BseBI CCWGG 2 cut(s) 327, 483
BseDI CCNNGG 5 cut(s) 18, 27, 204, 251, 474
BseGI GGATG 1 cut(s) 817
BseJI GATNNNNATC 1 cut(s) 38
BseMI GCAATG 2 cut(s) 286, 489
BseMII CTCAG 1 cut(s) 300
BseNI ACTGG 3 cut(s) 303, 596, 846
BseRI GAGGAG 2 cut(s) 448, 517
BseXI GCAGC 3 cut(s) 18, 506, 657
BseYI CCCAGC 1 cut(s) 497
BsgI GTGCAG 1 cut(s) 25
BshFI GGCC 3 cut(s) 17, 256, 845
BshNI GGYRCC 2 cut(s) 332, 478
BsiHKAI GWGCWC 1 cut(s) 760
BsiSI CCGG 1 cut(s) 115
BsmAI GTCTC 1 cut(s) 854
BsnI GGCC 3 cut(s) 17, 256, 845
Bsp1286I GDGCHC 1 cut(s) 760
Bsp143I GATC 8 cut(s) 272, 409, 468, 563, 631, 667, 691, 767
Bsp19I CCATGG 1 cut(s) 27
BspANI GGCC 3 cut(s) 17, 256, 845
BspCNI CTCAG 1 cut(s) 301
BspHI TCATGA 2 cut(s) 406, 715
BspLI GGNNCC 3 cut(s) 334, 480, 487
BspMI ACCTGC 2 cut(s) 98, 268
BspPI GGATC 2 cut(s) 662, 775
BspQI GCTCTTC 1 cut(s) 525
BspT107I GGYRCC 2 cut(s) 332, 478
BspTI CTTAAG 1 cut(s) 128
BsrDI GCAATG 2 cut(s) 286, 489
BsrFI RCCGGY 1 cut(s) 114
BsrI ACTGG 3 cut(s) 303, 596, 846
BssAI RCCGGY 1 cut(s) 114
BssECI CCNNGG 5 cut(s) 18, 27, 204, 251, 474
BssMI GATC 8 cut(s) 272, 409, 468, 563, 631, 667, 691, 767
BssT1I CCWWGG 3 cut(s) 18, 27, 204
Bst2UI CCWGG 2 cut(s) 327, 483
Bst4CI ACNGT 1 cut(s) 138
Bst6I CTCTTC 3 cut(s) 430, 466, 525
BstAFI CTTAAG 1 cut(s) 128
BstDEI CTNAG 4 cut(s) 12, 309, 345, 749
BstDSI CCRYGG 1 cut(s) 27
BstEII GGTNACC 1 cut(s) 22
BstF5I GGATG 1 cut(s) 817
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 8 cut(s) 275, 412, 471, 566, 634, 670, 694, 770
BstMAI GTCTC 1 cut(s) 854
BstMBI GATC 8 cut(s) 272, 409, 468, 563, 631, 667, 691, 767
BstMWI GCNNNNNNNGC 1 cut(s) 401
BstNI CCWGG 2 cut(s) 327, 483
BstNSI RCATGY 1 cut(s) 530
BstPI GGTNACC 1 cut(s) 22
BstSCI CCNGG 2 cut(s) 325, 481
BstV1I GCAGC 3 cut(s) 18, 506, 657
BstX2I RGATCY 1 cut(s) 667
BstYI RGATCY 1 cut(s) 667
BsuRI GGCC 3 cut(s) 17, 256, 845
BtgI CCRYGG 1 cut(s) 27
BtsCI GGATG 1 cut(s) 817
BtsIMutI CAGTG 1 cut(s) 852
BveI ACCTGC 2 cut(s) 98, 268
CciI TCATGA 2 cut(s) 406, 715
CfoI GCGC 1 cut(s) 120
Cfr10I RCCGGY 1 cut(s) 114
Csp6I GTAC 2 cut(s) 201, 333
CviAII CATG 6 cut(s) 28, 407, 413, 527, 689, 716
CviQI GTAC 2 cut(s) 201, 333
DdeI CTNAG 4 cut(s) 12, 309, 345, 749
DpnI GATC 8 cut(s) 274, 411, 470, 565, 633, 669, 693, 769
DpnII GATC 8 cut(s) 272, 409, 468, 563, 631, 667, 691, 767
DraI TTTAAA 1 cut(s) 789
EaeI YGGCCR 1 cut(s) 843
Eam1104I CTCTTC 3 cut(s) 430, 466, 525
EarI CTCTTC 3 cut(s) 430, 466, 525
Ecl136II GAGCTC 1 cut(s) 758
Eco130I CCWWGG 3 cut(s) 18, 27, 204
Eco24I GRGCYC 1 cut(s) 760
Eco53kI GAGCTC 1 cut(s) 758
Eco57I CTGAAG 2 cut(s) 65, 855
Eco91I GGTNACC 1 cut(s) 22
EcoICRI GAGCTC 1 cut(s) 758
EcoO65I GGTNACC 1 cut(s) 22
EcoRII CCWGG 2 cut(s) 325, 481
EcoT14I CCWWGG 3 cut(s) 18, 27, 204
EcoT22I ATGCAT 1 cut(s) 817
EcoT38I GRGCYC 1 cut(s) 760
ErhI CCWWGG 3 cut(s) 18, 27, 204
FaeI CATG 6 cut(s) 31, 410, 416, 530, 692, 719
FatI CATG 6 cut(s) 27, 406, 412, 526, 688, 715
FbaI TGATCA 3 cut(s) 409, 468, 691
FblI GTMKAC 1 cut(s) 418
Fnu4HI GCNGC 3 cut(s) 7, 495, 646
FokI GGATG 1 cut(s) 824
FriOI GRGCYC 1 cut(s) 760
Fsp4HI GCNGC 3 cut(s) 7, 495, 646
FspBI CTAG 5 cut(s) 57, 105, 110, 186, 192
GlaI GCGC 1 cut(s) 119
GluI GCNGC 3 cut(s) 7, 495, 646
GsaI CCCAGC 1 cut(s) 501
GsuI CTGGAG 2 cut(s) 286, 579
HaeIII GGCC 3 cut(s) 17, 256, 845
HapII CCGG 1 cut(s) 115
HhaI GCGC 1 cut(s) 120
Hin1II CATG 6 cut(s) 31, 410, 416, 530, 692, 719
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HincII GTYRAC 1 cut(s) 141
HindII GTYRAC 1 cut(s) 141
HindIII AAGCTT 1 cut(s) 380
HinfI GANTC 2 cut(s) 60, 420
HpaII CCGG 1 cut(s) 115
HphI GGTGA 3 cut(s) 34, 463, 635
Hpy166II GTNNAC 2 cut(s) 141, 419
Hpy188I TCNGA 6 cut(s) 252, 310, 468, 667, 772, 883
Hpy188III TCNNGA 4 cut(s) 57, 407, 695, 716
Hpy8I GTNNAC 2 cut(s) 141, 419
HpyCH4III ACNGT 1 cut(s) 138
HpyCH4V TGCA 7 cut(s) 6, 93, 494, 530, 575, 645, 815
HpyF10VI GCNNNNNNNGC 1 cut(s) 401
HpyF3I CTNAG 4 cut(s) 12, 309, 345, 749
Hsp92II CATG 6 cut(s) 31, 410, 416, 530, 692, 719
HspAI GCGC 1 cut(s) 118
KpnI GGTACC 1 cut(s) 336
Ksp22I TGATCA 3 cut(s) 409, 468, 691
Kzo9I GATC 8 cut(s) 272, 409, 468, 563, 631, 667, 691, 767
LguI GCTCTTC 1 cut(s) 525
LmnI GCTCC 3 cut(s) 508, 598, 755
Lsp1109I GCAGC 3 cut(s) 18, 506, 657
LweI GCATC 3 cut(s) 632, 802, 820
MaeI CTAG 5 cut(s) 57, 105, 110, 186, 192
MaeIII GTNAC 2 cut(s) 22, 319
MalI GATC 8 cut(s) 274, 411, 470, 565, 633, 669, 693, 769
MboI GATC 8 cut(s) 272, 409, 468, 563, 631, 667, 691, 767
MboII GAAGA 8 cut(s) 165, 373, 417, 453, 512, 573, 638, 641
MflI RGATCY 1 cut(s) 667
MhlI GDGCHC 1 cut(s) 760
MlsI TGGCCA 1 cut(s) 845
MluCI AATT 5 cut(s) 121, 195, 228, 577, 661
MluNI TGGCCA 1 cut(s) 845
MlyI GAGTC 1 cut(s) 414
MmeI TCCRAC 1 cut(s) 745
Mox20I TGGCCA 1 cut(s) 845
Mph1103I ATGCAT 1 cut(s) 817
MscI TGGCCA 1 cut(s) 845
MseI TTAA 3 cut(s) 129, 378, 788
MslI CAYNNNNRTG 1 cut(s) 411
Msp20I TGGCCA 1 cut(s) 845
MspA1I CMGCKG 1 cut(s) 497
MspCI CTTAAG 1 cut(s) 128
MspI CCGG 1 cut(s) 115
MspR9I CCNGG 2 cut(s) 327, 483
MvaI CCWGG 2 cut(s) 327, 483
MwoI GCNNNNNNNGC 1 cut(s) 401
NcoI CCATGG 1 cut(s) 27
NdeII GATC 8 cut(s) 272, 409, 468, 563, 631, 667, 691, 767
NlaIII CATG 6 cut(s) 31, 410, 416, 530, 692, 719
NlaIV GGNNCC 3 cut(s) 334, 480, 487
NmuCI GTSAC 2 cut(s) 22, 319
NsiI ATGCAT 1 cut(s) 817
NspI RCATGY 1 cut(s) 530
PagI TCATGA 2 cut(s) 406, 715
PaqCI CACCTGC 1 cut(s) 268
PciSI GCTCTTC 1 cut(s) 525
PfeI GAWTC 1 cut(s) 60
PkrI GCNGC 3 cut(s) 8, 496, 647
PleI GAGTC 1 cut(s) 414
PpsI GAGTC 1 cut(s) 414
Psp124BI GAGCTC 1 cut(s) 760
Psp6I CCWGG 2 cut(s) 325, 481
PspEI GGTNACC 1 cut(s) 22
PspFI CCCAGC 1 cut(s) 497
PspGI CCWGG 2 cut(s) 325, 481
PspN4I GGNNCC 3 cut(s) 334, 480, 487
PsuI RGATCY 1 cut(s) 667
PvuII CAGCTG 1 cut(s) 497
RsaI GTAC 2 cut(s) 202, 334
RsaNI GTAC 2 cut(s) 201, 333
RseI CAYNNNNRTG 1 cut(s) 411
SacI GAGCTC 1 cut(s) 760
SapI GCTCTTC 1 cut(s) 525
SaqAI TTAA 3 cut(s) 129, 378, 788
SatI GCNGC 3 cut(s) 7, 495, 646
Sau3AI GATC 8 cut(s) 272, 409, 468, 563, 631, 667, 691, 767
SchI GAGTC 1 cut(s) 414
ScrFI CCNGG 2 cut(s) 327, 483
SduI GDGCHC 1 cut(s) 760
SfaNI GCATC 3 cut(s) 632, 802, 820
SmiMI CAYNNNNRTG 1 cut(s) 411
SmlI CTYRAG 2 cut(s) 128, 759
SmoI CTYRAG 2 cut(s) 128, 759
SpeI ACTAGT 1 cut(s) 185
Sse9I AATT 5 cut(s) 121, 195, 228, 577, 661
SspMI CTAG 5 cut(s) 57, 105, 110, 186, 192
SstI GAGCTC 1 cut(s) 760
StyD4I CCNGG 2 cut(s) 325, 481
StyI CCWWGG 3 cut(s) 18, 27, 204
TaaI ACNGT 1 cut(s) 138
TaqI TCGA 3 cut(s) 97, 434, 872
TasI AATT 5 cut(s) 121, 195, 228, 577, 661
TfiI GAWTC 1 cut(s) 60
Tru1I TTAA 3 cut(s) 129, 378, 788
Tru9I TTAA 3 cut(s) 129, 378, 788
TscAI CASTG 1 cut(s) 859
TseFI GTSAC 2 cut(s) 22, 319
TseI GCWGC 3 cut(s) 6, 494, 645
Tsp45I GTSAC 2 cut(s) 22, 319
TspDTI ATGAA 3 cut(s) 201, 640, 704
TspRI CASTG 1 cut(s) 859
Vha464I CTTAAG 1 cut(s) 128
XapI RAATTY 1 cut(s) 577
XbaI TCTAGA 1 cut(s) 56
XceI RCATGY 1 cut(s) 530
XcmI CCANNNNNNNNNTGG 1 cut(s) 25
XmiI GTMKAC 1 cut(s) 418
XspI CTAG 5 cut(s) 57, 105, 110, 186, 192
Zsp2I ATGCAT 1 cut(s) 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.