RchiOBHm_Chr5g0023071

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0012861
Physical Location & Seq
Reverse (-)
17073776 .. 17076881
3106 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30297

Sequence Viewer

Length: 1236 bp
ATGTGCAGCTCTAAGGCCAAGGTGACCATGGGGATTGAAATCACACCCCTTGTTTCTAGAATCAACGGCAGACCAGTGCTTCAGCCTACCTGCAATCGAGTTCCTAGCCTAGACCGGCGCAATTCCCTTAAGAAACTGTCAACAGCACCACCACCTCTACCATTATCAACTTCTTCTTCTACCAGCACTAGTCCTAGAATTAGTACCAAGGCTTCATTGACAACACCACCAATTTCTCCAAAGTCAAAATCTCCGAGGCCACCTGCGATAAAGAGATCAGGCAATGACCCTAATGGCTTGAACTCCAGTTCTGAGAAAGTTGTGACACCAGGAGGTACCACAAGAGCTAAGGTTTTGGAGAGGAAGAAGTCAAAGAGTTTTAAGCTTGGTGTTGGTGCTGATAATGCTCATGATCATGGTAGACTCTCTTCTGCTTCGATTGAGGCTTCGTTGAGCTACTCCTCTTCTCTGATCACCGAGGCACCAGGAACCATTGCAGCTGGGAGGAGGGAGCAGATGGCTCTTCAACATGCACAGAGAAAGATGAGGATTGCTCATTATGGAAGATCAAAGTCTGCAAATTTTGAAAGGGTTGCTCCAGTTGATACTATGGAAGCCAAAGCTGGTGAAGAAGATCACAAAAGATGCAGCTTCATCACGGCTAATTCAGATCCCATATATGTTGCTTACCATGATCAAGAATGGGGAGTTCCTGTTCATGATGACAAGATGTTATTTGAACTGCTTGTTCTAAGTGGAGCTCAAGTTGGATCAGATTGGACTTCCATTTTAAAGAAACGCCAAGATTTCAGGGATGCATTTTCTGGGTTTGATGCTGAAGCTGTGGCCAGTCTCACTGACAAGCAAATGATTTCGATTTGTTCAGAATATGGCATTGATATAAGCAGAGTTAGAGGAGTAGTAGACAACTCTAACAGAATTCTTGAGGTAAAGAAGGAATTTGGGTCATTTCACAAATACATATGGGGGTTTGTGAATCACAAGCCAATTTCCCCTCAATACAAGCAAGGCTACAAAATCCCAGTGAAGACATCAAAATCAGAGAGCATAAGCAAAGACATGGTTAGGAGGGGGTTTAGGTTTGTTGGTCCAACAGTTGTTCATTCCTTCATGCAAGCCTCAGGCCTCACAAATGACCATTTGACCACCTGCCACAGGCACCTTCAGTGCACCTTACTAGCAGCCCATCCTACCACATTGGAACAAGCTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

411

Amino Acids

45.05

Weight (kDa)

9.78

Isoelectric Point (pI)

44.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 221 - 394 1.7e-65 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012861)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12710
fragaria_vesca FvH4_3g13890
malus_domestica MD05G1240400.v1.1 MD10G1218500.v1.1
prunus_persica Prupe.4G125700_v2.0.a1
pyrus_communis pycom05g21640 pycom10g18430
rosa_chinensis RchiOBHm_Chr5g0023071
rosa_laevigata RLG00000032698
rosa_multiflora Rmu_co8421393.1_g000001
rosa_roxburghii Rroxscaffold_1G00056250
rosa_rugosa Rorug05G0077100
rosa_samantha Rh5AG163200 Rh5BG162500 Rh5CG177300 Rh5DG163700
rosa_wichuraiana Rw5G014720 Rw5G014990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 271, 1178
Acc36I ACCTGC 3 cut(s) 98, 271, 1178
Acc65I GGTACC 1 cut(s) 335
AccB1I GGYRCC 3 cut(s) 335, 481, 1179
AccI GTMKAC 2 cut(s) 421, 924
AclWI GGATC 2 cut(s) 665, 778
AcoI YGGCCR 1 cut(s) 846
AcsI RAATTY 3 cut(s) 580, 939, 959
AcuI CTGAAG 3 cut(s) 65, 858, 1169
AfaI GTAC 2 cut(s) 205, 337
AfiI CCNNNNNNNGG 1 cut(s) 1176
AflII CTTAAG 1 cut(s) 128
AgsI TTSAA 5 cut(s) 38, 301, 527, 587, 740
AhlI ACTAGT 1 cut(s) 188
AjnI CCWGG 2 cut(s) 328, 484
AloI GAACNNNNNNTCC 2 cut(s) 699, 731
Alw21I GWGCWC 2 cut(s) 763, 1193
Alw26I GTCTC 1 cut(s) 857
Alw44I GTGCAC 1 cut(s) 1189
AlwI GGATC 2 cut(s) 665, 778
AoxI GGCC 4 cut(s) 15, 257, 846, 1144
ApaLI GTGCAC 1 cut(s) 1189
ApeKI GCWGC 4 cut(s) 6, 497, 648, 1202
ApoI RAATTY 3 cut(s) 580, 939, 959
Asp718I GGTACC 1 cut(s) 335
AspLEI GCGC 1 cut(s) 120
AspS9I GGNCC 1 cut(s) 1109
AsuHPI GGTGA 3 cut(s) 34, 466, 638
AvaII GGWCC 1 cut(s) 1109
AxyI CCTNAGG 1 cut(s) 1141
BaeGI GKGCMC 1 cut(s) 1193
BalI TGGCCA 1 cut(s) 848
BanI GGYRCC 3 cut(s) 335, 481, 1179
BanII GRGCYC 1 cut(s) 763
BarI GAAGNNNNNNTAC 4 cut(s) 196, 228, 412, 444
BbsI GAAGAC 1 cut(s) 1055
Bbv12I GWGCWC 2 cut(s) 763, 1193
BbvI GCAGC 4 cut(s) 18, 509, 660, 1214
BccI CCATC 2 cut(s) 511, 1215
BceAI ACGGC 2 cut(s) 82, 675
BciT130I CCWGG 2 cut(s) 330, 486
BclI TGATCA 3 cut(s) 412, 471, 694
BcoDI GTCTC 1 cut(s) 857
BcuI ACTAGT 1 cut(s) 188
BfaI CTAG 6 cut(s) 57, 105, 110, 189, 195, 1199
BfmI CTRYAG 1 cut(s) 1232
BfrI CTTAAG 1 cut(s) 128
BfuAI ACCTGC 3 cut(s) 98, 271, 1178
BisI GCNGC 4 cut(s) 7, 498, 649, 1203
BlsI GCNGC 4 cut(s) 8, 499, 650, 1204
Bme1390I CCNGG 2 cut(s) 330, 486
Bme18I GGWCC 1 cut(s) 1109
BmgT120I GGNCC 1 cut(s) 1109
BmiI GGNNCC 4 cut(s) 337, 483, 490, 1181
BmrFI CCNGG 2 cut(s) 330, 486
BmrI ACTGGG 1 cut(s) 1037
BmsI GCATC 3 cut(s) 635, 805, 823
BmuI ACTGGG 1 cut(s) 1037
BpiI GAAGAC 1 cut(s) 1055
BplI GAGNNNNNCTC 2 cut(s) 538, 570
BpmI CTGGAG 2 cut(s) 289, 582
Bpu10I CCTNAGC 1 cut(s) 348
BpuEI CTTGAG 2 cut(s) 747, 965
BsaBI GATNNNNATC 1 cut(s) 38
BsaJI CCNNGG 5 cut(s) 18, 27, 207, 254, 477
BsaXI ACNNNNNCTCC 4 cut(s) 699, 729, 750, 780
Bsc4I CCNNNNNNNGG 1 cut(s) 1176
Bse118I RCCGGY 1 cut(s) 114
Bse1I ACTGG 5 cut(s) 74, 306, 599, 849, 1043
Bse21I CCTNAGG 1 cut(s) 1141
Bse3DI GCAATG 2 cut(s) 289, 492
Bse8I GATNNNNATC 1 cut(s) 38
BseBI CCWGG 2 cut(s) 330, 486
BseDI CCNNGG 5 cut(s) 18, 27, 207, 254, 477
BseGI GGATG 2 cut(s) 820, 1207
BseJI GATNNNNATC 1 cut(s) 38
BseLI CCNNNNNNNGG 1 cut(s) 1176
BseMI GCAATG 2 cut(s) 289, 492
BseMII CTCAG 2 cut(s) 303, 1155
BseNI ACTGG 5 cut(s) 74, 306, 599, 849, 1043
BseRI GAGGAG 3 cut(s) 451, 520, 930
BseSI GKGCMC 1 cut(s) 1193
BseXI GCAGC 4 cut(s) 18, 509, 660, 1214
BseYI CCCAGC 1 cut(s) 500
BsgI GTGCAG 1 cut(s) 25
BshFI GGCC 4 cut(s) 17, 259, 848, 1146
BshNI GGYRCC 3 cut(s) 335, 481, 1179
BsiHKAI GWGCWC 2 cut(s) 763, 1193
BsiSI CCGG 1 cut(s) 115
BslI CCNNNNNNNGG 1 cut(s) 1176
BsmAI GTCTC 1 cut(s) 857
BsnI GGCC 4 cut(s) 17, 259, 848, 1146
Bsp1286I GDGCHC 2 cut(s) 763, 1193
Bsp143I GATC 8 cut(s) 275, 412, 471, 566, 634, 670, 694, 770
Bsp19I CCATGG 1 cut(s) 27
BspANI GGCC 4 cut(s) 17, 259, 848, 1146
BspCNI CTCAG 2 cut(s) 304, 1154
BspHI TCATGA 2 cut(s) 409, 718
BspLI GGNNCC 4 cut(s) 337, 483, 490, 1181
BspMI ACCTGC 3 cut(s) 98, 271, 1178
BspPI GGATC 2 cut(s) 665, 778
BspQI GCTCTTC 1 cut(s) 528
BspT107I GGYRCC 3 cut(s) 335, 481, 1179
BspTI CTTAAG 1 cut(s) 128
BsrDI GCAATG 2 cut(s) 289, 492
BsrFI RCCGGY 1 cut(s) 114
BsrI ACTGG 5 cut(s) 74, 306, 599, 849, 1043
BssAI RCCGGY 1 cut(s) 114
BssECI CCNNGG 5 cut(s) 18, 27, 207, 254, 477
BssMI GATC 8 cut(s) 275, 412, 471, 566, 634, 670, 694, 770
BssT1I CCWWGG 3 cut(s) 18, 27, 207
Bst2UI CCWGG 2 cut(s) 330, 486
Bst4CI ACNGT 2 cut(s) 138, 1117
Bst6I CTCTTC 3 cut(s) 433, 469, 528
BstAFI CTTAAG 1 cut(s) 128
BstC8I GCNNGC 1 cut(s) 1137
BstDEI CTNAG 5 cut(s) 12, 312, 348, 752, 1141
BstDSI CCRYGG 1 cut(s) 27
BstEII GGTNACC 1 cut(s) 22
BstENI CCTNNNNNAGG 1 cut(s) 1174
BstF5I GGATG 2 cut(s) 820, 1207
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 8 cut(s) 278, 415, 474, 569, 637, 673, 697, 773
BstMAI GTCTC 1 cut(s) 857
BstMBI GATC 8 cut(s) 275, 412, 471, 566, 634, 670, 694, 770
BstMWI GCNNNNNNNGC 1 cut(s) 404
BstNI CCWGG 2 cut(s) 330, 486
BstNSI RCATGY 1 cut(s) 533
BstPI GGTNACC 1 cut(s) 22
BstSCI CCNGG 2 cut(s) 328, 484
BstSFI CTRYAG 1 cut(s) 1232
BstSLI GKGCMC 1 cut(s) 1193
BstV1I GCAGC 4 cut(s) 18, 509, 660, 1214
BstV2I GAAGAC 1 cut(s) 1055
BstX2I RGATCY 1 cut(s) 670
BstYI RGATCY 1 cut(s) 670
Bsu36I CCTNAGG 1 cut(s) 1141
BsuRI GGCC 4 cut(s) 17, 259, 848, 1146
BtgI CCRYGG 1 cut(s) 27
BtsCI GGATG 2 cut(s) 820, 1207
BtsIMutI CAGTG 4 cut(s) 81, 855, 1050, 1193
BveI ACCTGC 3 cut(s) 98, 271, 1178
Cac8I GCNNGC 1 cut(s) 1137
CciI TCATGA 2 cut(s) 409, 718
CfoI GCGC 1 cut(s) 120
Cfr10I RCCGGY 1 cut(s) 114
Cfr13I GGNCC 1 cut(s) 1109
Csp6I GTAC 2 cut(s) 204, 336
CviAII CATG 8 cut(s) 28, 410, 416, 530, 692, 719, 1081, 1132
CviQI GTAC 2 cut(s) 204, 336
DdeI CTNAG 5 cut(s) 12, 312, 348, 752, 1141
DpnI GATC 8 cut(s) 277, 414, 473, 568, 636, 672, 696, 772
DpnII GATC 8 cut(s) 275, 412, 471, 566, 634, 670, 694, 770
DraI TTTAAA 1 cut(s) 792
EaeI YGGCCR 1 cut(s) 846
Eam1104I CTCTTC 3 cut(s) 433, 469, 528
EarI CTCTTC 3 cut(s) 433, 469, 528
Ecl136II GAGCTC 1 cut(s) 761
Eco130I CCWWGG 3 cut(s) 18, 27, 207
Eco147I AGGCCT 1 cut(s) 1146
Eco24I GRGCYC 1 cut(s) 763
Eco47I GGWCC 1 cut(s) 1109
Eco53kI GAGCTC 1 cut(s) 761
Eco57I CTGAAG 3 cut(s) 65, 858, 1169
Eco81I CCTNAGG 1 cut(s) 1141
Eco91I GGTNACC 1 cut(s) 22
EcoICRI GAGCTC 1 cut(s) 761
EcoNI CCTNNNNNAGG 1 cut(s) 1174
EcoO65I GGTNACC 1 cut(s) 22
EcoRI GAATTC 1 cut(s) 939
EcoRII CCWGG 2 cut(s) 328, 484
EcoT14I CCWWGG 3 cut(s) 18, 27, 207
EcoT22I ATGCAT 1 cut(s) 820
EcoT38I GRGCYC 1 cut(s) 763
ErhI CCWWGG 3 cut(s) 18, 27, 207
FaeI CATG 8 cut(s) 31, 413, 419, 533, 695, 722, 1084, 1135
FatI CATG 8 cut(s) 27, 409, 415, 529, 691, 718, 1080, 1131
FauNDI CATATG 1 cut(s) 983
FbaI TGATCA 3 cut(s) 412, 471, 694
FblI GTMKAC 2 cut(s) 421, 924
Fnu4HI GCNGC 4 cut(s) 7, 498, 649, 1203
FokI GGATG 2 cut(s) 827, 1194
FriOI GRGCYC 1 cut(s) 763
Fsp4HI GCNGC 4 cut(s) 7, 498, 649, 1203
FspBI CTAG 6 cut(s) 57, 105, 110, 189, 195, 1199
GlaI GCGC 1 cut(s) 119
GluI GCNGC 4 cut(s) 7, 498, 649, 1203
GsaI CCCAGC 1 cut(s) 504
GsuI CTGGAG 2 cut(s) 289, 582
HaeIII GGCC 4 cut(s) 17, 259, 848, 1146
HapII CCGG 1 cut(s) 115
HhaI GCGC 1 cut(s) 120
Hin1II CATG 8 cut(s) 31, 413, 419, 533, 695, 722, 1084, 1135
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HincII GTYRAC 1 cut(s) 141
HindII GTYRAC 1 cut(s) 141
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 3 cut(s) 60, 423, 997
HpaII CCGG 1 cut(s) 115
HphI GGTGA 3 cut(s) 34, 466, 638
Hpy166II GTNNAC 4 cut(s) 141, 422, 925, 1191
Hpy188I TCNGA 7 cut(s) 255, 313, 471, 670, 775, 886, 1063
Hpy188III TCNNGA 5 cut(s) 57, 410, 698, 719, 944
Hpy8I GTNNAC 4 cut(s) 141, 422, 925, 1191
HpyAV CCTTC 3 cut(s) 949, 1138, 1193
HpyCH4III ACNGT 2 cut(s) 138, 1117
HpyCH4V TGCA 9 cut(s) 6, 93, 497, 533, 578, 648, 818, 1135, 1191
HpyF10VI GCNNNNNNNGC 1 cut(s) 404
HpyF3I CTNAG 5 cut(s) 12, 312, 348, 752, 1141
Hsp92II CATG 8 cut(s) 31, 413, 419, 533, 695, 722, 1084, 1135
HspAI GCGC 1 cut(s) 118
KpnI GGTACC 1 cut(s) 339
Ksp22I TGATCA 3 cut(s) 412, 471, 694
Kzo9I GATC 8 cut(s) 275, 412, 471, 566, 634, 670, 694, 770
LguI GCTCTTC 1 cut(s) 528
LmnI GCTCC 3 cut(s) 511, 601, 758
Lsp1109I GCAGC 4 cut(s) 18, 509, 660, 1214
LweI GCATC 3 cut(s) 635, 805, 823
MaeI CTAG 6 cut(s) 57, 105, 110, 189, 195, 1199
MaeIII GTNAC 2 cut(s) 22, 322
MalI GATC 8 cut(s) 277, 414, 473, 568, 636, 672, 696, 772
MboI GATC 8 cut(s) 275, 412, 471, 566, 634, 670, 694, 770
MflI RGATCY 1 cut(s) 670
MhlI GDGCHC 2 cut(s) 763, 1193
MlsI TGGCCA 1 cut(s) 848
MluCI AATT 8 cut(s) 121, 198, 231, 580, 664, 939, 959, 1008
MluNI TGGCCA 1 cut(s) 848
MlyI GAGTC 1 cut(s) 417
MmeI TCCRAC 2 cut(s) 748, 1136
Mox20I TGGCCA 1 cut(s) 848
Mph1103I ATGCAT 1 cut(s) 820
MscI TGGCCA 1 cut(s) 848
MseI TTAA 3 cut(s) 129, 381, 791
MslI CAYNNNNRTG 1 cut(s) 414
Msp20I TGGCCA 1 cut(s) 848
MspA1I CMGCKG 1 cut(s) 500
MspCI CTTAAG 1 cut(s) 128
MspI CCGG 1 cut(s) 115
MspR9I CCNGG 2 cut(s) 330, 486
MvaI CCWGG 2 cut(s) 330, 486
MwoI GCNNNNNNNGC 1 cut(s) 404
NcoI CCATGG 1 cut(s) 27
NdeI CATATG 1 cut(s) 983
NdeII GATC 8 cut(s) 275, 412, 471, 566, 634, 670, 694, 770
NlaIII CATG 8 cut(s) 31, 413, 419, 533, 695, 722, 1084, 1135
NlaIV GGNNCC 4 cut(s) 337, 483, 490, 1181
NmuCI GTSAC 2 cut(s) 22, 322
NsiI ATGCAT 1 cut(s) 820
NspI RCATGY 1 cut(s) 533
PagI TCATGA 2 cut(s) 409, 718
PaqCI CACCTGC 2 cut(s) 271, 1178
PceI AGGCCT 1 cut(s) 1146
PciSI GCTCTTC 1 cut(s) 528
PfeI GAWTC 2 cut(s) 60, 997
PkrI GCNGC 4 cut(s) 8, 499, 650, 1204
PleI GAGTC 1 cut(s) 417
PpsI GAGTC 1 cut(s) 417
Psp124BI GAGCTC 1 cut(s) 763
Psp6I CCWGG 2 cut(s) 328, 484
PspEI GGTNACC 1 cut(s) 22
PspFI CCCAGC 1 cut(s) 500
PspGI CCWGG 2 cut(s) 328, 484
PspN4I GGNNCC 4 cut(s) 337, 483, 490, 1181
PspPI GGNCC 1 cut(s) 1109
PsuI RGATCY 1 cut(s) 670
PvuII CAGCTG 1 cut(s) 500
RsaI GTAC 2 cut(s) 205, 337
RsaNI GTAC 2 cut(s) 204, 336
RseI CAYNNNNRTG 1 cut(s) 414
SacI GAGCTC 1 cut(s) 763
SapI GCTCTTC 1 cut(s) 528
SaqAI TTAA 3 cut(s) 129, 381, 791
SatI GCNGC 4 cut(s) 7, 498, 649, 1203
Sau3AI GATC 8 cut(s) 275, 412, 471, 566, 634, 670, 694, 770
Sau96I GGNCC 1 cut(s) 1109
SchI GAGTC 1 cut(s) 417
ScrFI CCNGG 2 cut(s) 330, 486
SduI GDGCHC 2 cut(s) 763, 1193
SfaNI GCATC 3 cut(s) 635, 805, 823
SfcI CTRYAG 1 cut(s) 1232
SinI GGWCC 1 cut(s) 1109
SmiMI CAYNNNNRTG 1 cut(s) 414
SmlI CTYRAG 3 cut(s) 128, 762, 944
SmoI CTYRAG 3 cut(s) 128, 762, 944
SpeI ACTAGT 1 cut(s) 188
Sse9I AATT 8 cut(s) 121, 198, 231, 580, 664, 939, 959, 1008
SseBI AGGCCT 1 cut(s) 1146
SspMI CTAG 6 cut(s) 57, 105, 110, 189, 195, 1199
SstI GAGCTC 1 cut(s) 763
StuI AGGCCT 1 cut(s) 1146
StyD4I CCNGG 2 cut(s) 328, 484
StyI CCWWGG 3 cut(s) 18, 27, 207
TaaI ACNGT 2 cut(s) 138, 1117
TaqI TCGA 3 cut(s) 97, 437, 875
TasI AATT 8 cut(s) 121, 198, 231, 580, 664, 939, 959, 1008
TfiI GAWTC 2 cut(s) 60, 997
Tru1I TTAA 3 cut(s) 129, 381, 791
Tru9I TTAA 3 cut(s) 129, 381, 791
TscAI CASTG 4 cut(s) 81, 862, 1050, 1193
TseFI GTSAC 2 cut(s) 22, 322
TseI GCWGC 4 cut(s) 6, 497, 648, 1202
Tsp45I GTSAC 2 cut(s) 22, 322
TspDTI ATGAA 5 cut(s) 204, 643, 707, 1112, 1120
TspRI CASTG 4 cut(s) 81, 862, 1050, 1193
Vha464I CTTAAG 1 cut(s) 128
VneI GTGCAC 1 cut(s) 1189
VpaK11BI GGWCC 1 cut(s) 1109
XagI CCTNNNNNAGG 1 cut(s) 1174
XapI RAATTY 3 cut(s) 580, 939, 959
XbaI TCTAGA 1 cut(s) 56
XceI RCATGY 1 cut(s) 533
XcmI CCANNNNNNNNNTGG 1 cut(s) 25
XmiI GTMKAC 2 cut(s) 421, 924
XspI CTAG 6 cut(s) 57, 105, 110, 189, 195, 1199
Zsp2I ATGCAT 1 cut(s) 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.