pycom05g21640

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0012861
Physical Location & Seq
Forward (+)
24211414 .. 24213803
2390 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g21640.4

Sequence Viewer

Length: 1233 bp
ATGTGCAGCTCAAAGGCCAAGGTGACCTTAGGGATTGAAGTCACACCCATTGTGGCTAGAATCAATGGCAGGCCAGTCCTTCAGCCTAATTGCAACCGGGTTCCTAGCCTAGACCGGCGTGGTTCAATTAAGAGAATATCGACGCCACCACCACCTCCACTACCATTGCCGGCTTCTTCTGCTAGTACAAGTCCTAGAACTACCAACAAGGCCTCACCATTGTTAACACCTCCCATTTCTCCAGAATCGAAGTCTCCACGGCCACCAGCAATCAAGAGAGGAAATGACCCTAATGGGTTAAATTCGAGTTCCGAAAAAGTTGTGACACCAGGAGGTACCACAAGAGCAAAGATATTGGAGAGGAAGAAGTCCAAGAGTTTTAAGCGGGCTAGCGTTGGTGTTGATGTTGGTGCTGATCATGGCTGCTTTAGTAATGGTGGATTTTCTGCTTCGAATATCGAGGCGTCGTTGAGTTATTCGTCTTCTTTGATCATTGAGGCACCTGGCAGCATTGCTGCTGGGAGGAGGGAGCAGATGGCGCTTCAACATGCACAGAGAAAGATGAGAATTGCGCATTACGGTAGGTCAAAGTCTGCCAACTTTGAGAGGGTTGTCCCTGTTGATGCTTCTGGTAATGTAGAAGCCAAGGCCGCTGAAGAAGAGAAAAGATGCAGCTTTATCACGGCTAACTCAGATCCCATCTATGTTGCTTACCATGATGAAGAATGGGGAGTTCCTGTCCATGATGACAAACGGAATCAAACACTTATGTTTCTAAGAAATTGCATTTTTATATTCCAACTCTGCAGGAATGCATTTTCAGGATTTGATGCAGAAACTGTGGCAAATTTAAGTGAGAAACAGATGATGTCAATTGCTTCAGAATATGGCATTGAAATCAGCAGGATCCGAGGAGTTGTAGACAACTCTAACAGGATTCTTGAGATTAAGAAGGAATTTGGATCATTTGACAAGTACATCTGGGGATTTGTCAATCAAAAGCCCATCTCACCACAGTATAAATTAGGCTACAAAATCCCAGTGAAGACTTCAAAATCAGAGTCGATTAGCAAAGACATGGTTCGGAGGGGATTTAGGTTTGTGGGTCCAACAGTCGTTCATTCATTCATGCAAGCCTCAGGCGTCACGAATGACCATCTGATCACTTGCCATAGGCACCTCCAATGCACCTTATTGGCAGCCCGCCGCCCGGCATTGGAAGAAGTTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

411

Amino Acids

44.98

Weight (kDa)

9.77

Isoelectric Point (pI)

53.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012861)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12710
fragaria_vesca FvH4_3g13890
malus_domestica MD05G1240400.v1.1 MD10G1218500.v1.1
prunus_persica Prupe.4G125700_v2.0.a1
pyrus_communis pycom05g21640 pycom10g18430
rosa_chinensis RchiOBHm_Chr5g0023071
rosa_laevigata RLG00000032698
rosa_multiflora Rmu_co8421393.1_g000001
rosa_roxburghii Rroxscaffold_1G00056250
rosa_rugosa Rorug05G0077100
rosa_samantha Rh5AG163200 Rh5BG162500 Rh5CG177300 Rh5DG163700
rosa_wichuraiana Rw5G014720 Rw5G014990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 573
Acc65I GGTACC 1 cut(s) 335
AccB1I GGYRCC 3 cut(s) 335, 499, 1176
AccI GTMKAC 1 cut(s) 921
AciI CCGC 4 cut(s) 385, 651, 1204, 1207
AclWI GGATC 4 cut(s) 689, 901, 914, 970
AcoI YGGCCR 1 cut(s) 260
AcsI RAATTY 3 cut(s) 301, 847, 956
AcuI CTGAAG 3 cut(s) 65, 675, 864
AcyI GRCGYC 3 cut(s) 143, 464, 1143
AfaI GTAC 3 cut(s) 187, 337, 977
AfiI CCNNNNNNNGG 2 cut(s) 1210, 1216
AgsI TTSAA 5 cut(s) 38, 126, 545, 896, 1053
AjnI CCWGG 2 cut(s) 328, 502
AluBI AGCT 2 cut(s) 9, 675
AluI AGCT 2 cut(s) 9, 675
Alw26I GTCTC 1 cut(s) 258
AlwI GGATC 4 cut(s) 689, 901, 914, 970
AlwNI CAGNNNCTG 1 cut(s) 839
AoxI GGCC 5 cut(s) 15, 71, 210, 260, 648
ApeKI GCWGC 6 cut(s) 6, 423, 507, 515, 672, 1199
ApoI RAATTY 3 cut(s) 301, 847, 956
ArsI GACNNNNNNTTYG 2 cut(s) 1046, 1078
Asp700I GAANNNNTTC 1 cut(s) 1224
Asp718I GGTACC 1 cut(s) 335
AspLEI GCGC 2 cut(s) 541, 574
AspS9I GGNCC 1 cut(s) 1106
AsuC2I CCSGG 2 cut(s) 98, 1211
AsuHPI GGTGA 3 cut(s) 34, 207, 1002
AsuII TTCGAA 1 cut(s) 452
AsuNHI GCTAGC 1 cut(s) 389
AvaII GGWCC 1 cut(s) 1106
AxyI CCTNAGG 2 cut(s) 28, 1138
BamHI GGATCC 1 cut(s) 906
BanI GGYRCC 3 cut(s) 335, 499, 1176
BbsI GAAGAC 2 cut(s) 474, 1052
BbvI GCAGC 6 cut(s) 18, 410, 502, 519, 684, 1211
BccI CCATC 4 cut(s) 529, 707, 1013, 1164
BceAI ACGGC 2 cut(s) 275, 699
BciT130I CCWGG 2 cut(s) 330, 504
BclI TGATCA 3 cut(s) 415, 489, 1161
BcnI CCSGG 2 cut(s) 98, 1211
BcoDI GTCTC 1 cut(s) 258
BfaI CTAG 6 cut(s) 57, 105, 110, 183, 195, 390
BfmI CTRYAG 2 cut(s) 805, 1229
BfoI RGCGCY 1 cut(s) 542
BisI GCNGC 8 cut(s) 7, 424, 508, 516, 651, 673, 1200, 1207
BlsI GCNGC 8 cut(s) 8, 425, 509, 517, 652, 674, 1201, 1208
Bme1390I CCNGG 4 cut(s) 98, 330, 504, 1211
Bme18I GGWCC 1 cut(s) 1106
BmgT120I GGNCC 1 cut(s) 1106
BmiI GGNNCC 6 cut(s) 102, 337, 501, 908, 1107, 1178
BmrFI CCNGG 4 cut(s) 98, 330, 504, 1211
BmrI ACTGGG 1 cut(s) 1034
BmsI GCATC 3 cut(s) 613, 659, 820
BmtI GCTAGC 1 cut(s) 393
BmuI ACTGGG 1 cut(s) 1034
BpiI GAAGAC 2 cut(s) 474, 1052
BpmI CTGGAG 1 cut(s) 225
Bpu14I TTCGAA 1 cut(s) 452
BpuEI CTTGAG 1 cut(s) 962
BpuMI CCSGG 2 cut(s) 98, 1211
BsaHI GRCGYC 3 cut(s) 143, 464, 1143
BsaJI CCNNGG 4 cut(s) 18, 257, 645, 910
BsaXI ACNNNNNCTCC 2 cut(s) 723, 753
Bsc4I CCNNNNNNNGG 2 cut(s) 1210, 1216
Bse118I RCCGGY 2 cut(s) 114, 169
Bse1I ACTGG 2 cut(s) 74, 1040
Bse21I CCTNAGG 2 cut(s) 28, 1138
Bse3DI GCAATG 2 cut(s) 164, 510
BseBI CCWGG 2 cut(s) 330, 504
BseDI CCNNGG 4 cut(s) 18, 257, 645, 910
BseLI CCNNNNNNNGG 2 cut(s) 1210, 1216
BseMI GCAATG 2 cut(s) 164, 510
BseMII CTCAG 2 cut(s) 705, 1152
BseNI ACTGG 2 cut(s) 74, 1040
BseRI GAGGAG 2 cut(s) 538, 927
BseXI GCAGC 6 cut(s) 18, 410, 502, 519, 684, 1211
BseYI CCCAGC 1 cut(s) 518
BsgI GTGCAG 1 cut(s) 25
BshFI GGCC 5 cut(s) 17, 73, 212, 262, 650
BshNI GGYRCC 3 cut(s) 335, 499, 1176
BsiSI CCGG 4 cut(s) 97, 115, 170, 1211
BslFI GGGAC 1 cut(s) 599
BslI CCNNNNNNNGG 2 cut(s) 1210, 1216
BsmAI GTCTC 1 cut(s) 258
BsmFI GGGAC 1 cut(s) 599
BsmI GAATGC 1 cut(s) 817
BsnI GGCC 5 cut(s) 17, 73, 212, 262, 650
Bsp119I TTCGAA 1 cut(s) 452
Bsp143I GATC 6 cut(s) 415, 489, 694, 906, 962, 1161
BspACI CCGC 4 cut(s) 385, 651, 1204, 1207
BspANI GGCC 5 cut(s) 17, 73, 212, 262, 650
BspCNI CTCAG 2 cut(s) 704, 1151
BspLI GGNNCC 6 cut(s) 102, 337, 501, 908, 1107, 1178
BspMAI CTGCAG 1 cut(s) 809
BspOI GCTAGC 1 cut(s) 393
BspPI GGATC 4 cut(s) 689, 901, 914, 970
BspT104I TTCGAA 1 cut(s) 452
BspT107I GGYRCC 3 cut(s) 335, 499, 1176
BsrDI GCAATG 2 cut(s) 164, 510
BsrFI RCCGGY 2 cut(s) 114, 169
BsrI ACTGG 2 cut(s) 74, 1040
BssAI RCCGGY 2 cut(s) 114, 169
BssECI CCNNGG 4 cut(s) 18, 257, 645, 910
BssMI GATC 6 cut(s) 415, 489, 694, 906, 962, 1161
BssNI GRCGYC 3 cut(s) 143, 464, 1143
BssT1I CCWWGG 2 cut(s) 18, 645
Bst2UI CCWGG 2 cut(s) 330, 504
Bst4CI ACNGT 4 cut(s) 581, 841, 1017, 1114
Bst6I CTCTTC 1 cut(s) 654
BstACI GRCGYC 3 cut(s) 143, 464, 1143
BstBI TTCGAA 1 cut(s) 452
BstC8I GCNNGC 6 cut(s) 71, 171, 387, 391, 1134, 1204
BstDEI CTNAG 4 cut(s) 28, 691, 776, 1138
BstDSI CCRYGG 1 cut(s) 257
BstEII GGTNACC 1 cut(s) 22
BstH2I RGCGCY 1 cut(s) 542
BstHHI GCGC 2 cut(s) 541, 574
BstKTI GATC 6 cut(s) 418, 492, 697, 909, 965, 1164
BstMAI GTCTC 1 cut(s) 258
BstMBI GATC 6 cut(s) 415, 489, 694, 906, 962, 1161
BstMWI GCNNNNNNNGC 3 cut(s) 179, 538, 650
BstNI CCWGG 2 cut(s) 330, 504
BstNSI RCATGY 1 cut(s) 551
BstPI GGTNACC 1 cut(s) 22
BstSCI CCNGG 4 cut(s) 96, 328, 502, 1209
BstSFI CTRYAG 2 cut(s) 805, 1229
BstV1I GCAGC 6 cut(s) 18, 410, 502, 519, 684, 1211
BstV2I GAAGAC 2 cut(s) 474, 1052
BstX2I RGATCY 2 cut(s) 694, 906
BstYI RGATCY 2 cut(s) 694, 906
Bsu36I CCTNAGG 2 cut(s) 28, 1138
BsuRI GGCC 5 cut(s) 17, 73, 212, 262, 650
BtgI CCRYGG 1 cut(s) 257
BtsIMutI CAGTG 1 cut(s) 1047
Cac8I GCNNGC 6 cut(s) 71, 171, 387, 391, 1134, 1204
CaiI CAGNNNCTG 1 cut(s) 839
CfoI GCGC 2 cut(s) 541, 574
Cfr10I RCCGGY 2 cut(s) 114, 169
Cfr13I GGNCC 1 cut(s) 1106
CseI GACGC 3 cut(s) 151, 453, 1132
Csp6I GTAC 3 cut(s) 186, 336, 976
CspCI CAANNNNNGTGG 2 cut(s) 147, 182
CviAII CATG 6 cut(s) 419, 548, 716, 743, 1078, 1129
CviQI GTAC 3 cut(s) 186, 336, 976
DdeI CTNAG 4 cut(s) 28, 691, 776, 1138
DpnI GATC 6 cut(s) 417, 491, 696, 908, 964, 1163
DpnII GATC 6 cut(s) 415, 489, 694, 906, 962, 1161
EaeI YGGCCR 1 cut(s) 260
Eam1104I CTCTTC 1 cut(s) 654
EarI CTCTTC 1 cut(s) 654
Eco130I CCWWGG 2 cut(s) 18, 645
Eco147I AGGCCT 1 cut(s) 212
Eco47I GGWCC 1 cut(s) 1106
Eco57I CTGAAG 3 cut(s) 65, 675, 864
Eco81I CCTNAGG 2 cut(s) 28, 1138
Eco91I GGTNACC 1 cut(s) 22
EcoO65I GGTNACC 1 cut(s) 22
EcoRII CCWGG 2 cut(s) 328, 502
EcoT14I CCWWGG 2 cut(s) 18, 645
EcoT22I ATGCAT 1 cut(s) 817
ErhI CCWWGG 2 cut(s) 18, 645
FaeI CATG 6 cut(s) 422, 551, 719, 746, 1081, 1132
FalI AAGNNNNNCTT 2 cut(s) 11, 43
FaqI GGGAC 1 cut(s) 599
FatI CATG 6 cut(s) 418, 547, 715, 742, 1077, 1128
FauI CCCGC 2 cut(s) 378, 1211
FbaI TGATCA 3 cut(s) 415, 489, 1161
FblI GTMKAC 1 cut(s) 921
Fnu4HI GCNGC 8 cut(s) 7, 424, 508, 516, 651, 673, 1200, 1207
Fsp4HI GCNGC 8 cut(s) 7, 424, 508, 516, 651, 673, 1200, 1207
FspBI CTAG 6 cut(s) 57, 105, 110, 183, 195, 390
FspI TGCGCA 1 cut(s) 573
GlaI GCGC 2 cut(s) 540, 573
GluI GCNGC 8 cut(s) 7, 424, 508, 516, 651, 673, 1200, 1207
GsaI CCCAGC 1 cut(s) 522
GsuI CTGGAG 1 cut(s) 225
HaeII RGCGCY 1 cut(s) 542
HaeIII GGCC 5 cut(s) 17, 73, 212, 262, 650
HapII CCGG 4 cut(s) 97, 115, 170, 1211
HgaI GACGC 3 cut(s) 151, 453, 1132
HhaI GCGC 2 cut(s) 541, 574
Hin1I GRCGYC 3 cut(s) 143, 464, 1143
Hin1II CATG 6 cut(s) 422, 551, 719, 746, 1081, 1132
Hin6I GCGC 2 cut(s) 539, 572
HinP1I GCGC 2 cut(s) 539, 572
HincII GTYRAC 1 cut(s) 225
HindII GTYRAC 1 cut(s) 225
HinfI GANTC 5 cut(s) 60, 245, 757, 937, 1061
HpaI GTTAAC 1 cut(s) 225
HpaII CCGG 4 cut(s) 97, 115, 170, 1211
HphI GGTGA 3 cut(s) 34, 207, 1002
Hpy166II GTNNAC 2 cut(s) 225, 922
Hpy188I TCNGA 7 cut(s) 313, 694, 883, 911, 1060, 1086, 1161
Hpy188III TCNNGA 5 cut(s) 242, 274, 822, 941, 1147
Hpy8I GTNNAC 2 cut(s) 225, 922
Hpy99I CGWCG 2 cut(s) 145, 469
HpyAV CCTTC 2 cut(s) 89, 946
HpyCH4III ACNGT 4 cut(s) 581, 841, 1017, 1114
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 538, 650
HpyF3I CTNAG 4 cut(s) 28, 691, 776, 1138
Hsp92I GRCGYC 3 cut(s) 143, 464, 1143
Hsp92II CATG 6 cut(s) 422, 551, 719, 746, 1081, 1132
HspAI GCGC 2 cut(s) 539, 572
KpnI GGTACC 1 cut(s) 339
KroI GCCGGC 1 cut(s) 169
KroNI GCCGGC 1 cut(s) 171
Ksp22I TGATCA 3 cut(s) 415, 489, 1161
KspAI GTTAAC 1 cut(s) 225
Kzo9I GATC 6 cut(s) 415, 489, 694, 906, 962, 1161
LmnI GCTCC 1 cut(s) 529
Lsp1109I GCAGC 6 cut(s) 18, 410, 502, 519, 684, 1211
LweI GCATC 3 cut(s) 613, 659, 820
MaeI CTAG 6 cut(s) 57, 105, 110, 183, 195, 390
MaeIII GTNAC 4 cut(s) 22, 40, 322, 1144
MalI GATC 6 cut(s) 417, 491, 696, 908, 964, 1163
MboI GATC 6 cut(s) 415, 489, 694, 906, 962, 1161
MboII GAAGA 8 cut(s) 168, 376, 474, 668, 671, 734, 1057, 1232
MfeI CAATTG 1 cut(s) 873
MflI RGATCY 2 cut(s) 694, 906
MluCI AATT 9 cut(s) 88, 126, 301, 567, 781, 847, 873, 956, 1022
MlyI GAGTC 1 cut(s) 1070
MmeI TCCRAC 2 cut(s) 823, 1133
Mph1103I ATGCAT 1 cut(s) 817
MroNI GCCGGC 1 cut(s) 169
MroXI GAANNNNTTC 1 cut(s) 1224
MseI TTAA 6 cut(s) 129, 224, 299, 381, 851, 948
MspA1I CMGCKG 1 cut(s) 653
MspI CCGG 4 cut(s) 97, 115, 170, 1211
MspR9I CCNGG 4 cut(s) 98, 330, 504, 1211
MunI CAATTG 1 cut(s) 873
Mva1269I GAATGC 1 cut(s) 817
MvaI CCWGG 2 cut(s) 330, 504
MwoI GCNNNNNNNGC 3 cut(s) 179, 538, 650
NaeI GCCGGC 1 cut(s) 171
NciI CCSGG 2 cut(s) 98, 1211
NdeII GATC 6 cut(s) 415, 489, 694, 906, 962, 1161
NgoMIV GCCGGC 1 cut(s) 169
NheI GCTAGC 1 cut(s) 389
NlaIII CATG 6 cut(s) 422, 551, 719, 746, 1081, 1132
NlaIV GGNNCC 6 cut(s) 102, 337, 501, 908, 1107, 1178
NmuCI GTSAC 4 cut(s) 22, 40, 322, 1144
NsbI TGCGCA 1 cut(s) 573
NsiI ATGCAT 1 cut(s) 817
NspI RCATGY 1 cut(s) 551
NspV TTCGAA 1 cut(s) 452
PceI AGGCCT 1 cut(s) 212
PctI GAATGC 1 cut(s) 817
PdiI GCCGGC 1 cut(s) 171
PdmI GAANNNNTTC 1 cut(s) 1224
PfeI GAWTC 4 cut(s) 60, 245, 757, 937
PkrI GCNGC 8 cut(s) 8, 425, 509, 517, 652, 674, 1201, 1208
PleI GAGTC 1 cut(s) 1069
PpsI GAGTC 1 cut(s) 1069
Psp6I CCWGG 2 cut(s) 328, 502
PspEI GGTNACC 1 cut(s) 22
PspFI CCCAGC 1 cut(s) 518
PspGI CCWGG 2 cut(s) 328, 502
PspN4I GGNNCC 6 cut(s) 102, 337, 501, 908, 1107, 1178
PspPI GGNCC 1 cut(s) 1106
PstI CTGCAG 1 cut(s) 809
PstNI CAGNNNCTG 1 cut(s) 839
PsuI RGATCY 2 cut(s) 694, 906
RsaI GTAC 3 cut(s) 187, 337, 977
RsaNI GTAC 3 cut(s) 186, 336, 976
SaqAI TTAA 6 cut(s) 129, 224, 299, 381, 851, 948
SatI GCNGC 8 cut(s) 7, 424, 508, 516, 651, 673, 1200, 1207
Sau3AI GATC 6 cut(s) 415, 489, 694, 906, 962, 1161
Sau96I GGNCC 1 cut(s) 1106
SchI GAGTC 1 cut(s) 1070
ScrFI CCNGG 4 cut(s) 98, 330, 504, 1211
SfaNI GCATC 3 cut(s) 613, 659, 820
SfcI CTRYAG 2 cut(s) 805, 1229
SfuI TTCGAA 1 cut(s) 452
SinI GGWCC 1 cut(s) 1106
SmlI CTYRAG 1 cut(s) 941
SmoI CTYRAG 1 cut(s) 941
Sse9I AATT 9 cut(s) 88, 126, 301, 567, 781, 847, 873, 956, 1022
SseBI AGGCCT 1 cut(s) 212
SsiI CCGC 4 cut(s) 385, 651, 1204, 1207
SspMI CTAG 6 cut(s) 57, 105, 110, 183, 195, 390
StuI AGGCCT 1 cut(s) 212
StyD4I CCNGG 4 cut(s) 96, 328, 502, 1209
StyI CCWWGG 2 cut(s) 18, 645
TaaI ACNGT 4 cut(s) 581, 841, 1017, 1114
TaqI TCGA 6 cut(s) 140, 248, 305, 452, 459, 1064
TasI AATT 9 cut(s) 88, 126, 301, 567, 781, 847, 873, 956, 1022
TatI WGTACW 2 cut(s) 185, 975
TauI GCSGC 2 cut(s) 653, 1209
TfiI GAWTC 4 cut(s) 60, 245, 757, 937
Tru1I TTAA 6 cut(s) 129, 224, 299, 381, 851, 948
Tru9I TTAA 6 cut(s) 129, 224, 299, 381, 851, 948
TscAI CASTG 1 cut(s) 1047
TseFI GTSAC 4 cut(s) 22, 40, 322, 1144
TseI GCWGC 6 cut(s) 6, 423, 507, 515, 672, 1199
Tsp45I GTSAC 4 cut(s) 22, 40, 322, 1144
TspDTI ATGAA 4 cut(s) 735, 1109, 1113, 1117
TspGWI ACGGA 1 cut(s) 769
TspRI CASTG 1 cut(s) 1047
VpaK11BI GGWCC 1 cut(s) 1106
XapI RAATTY 3 cut(s) 301, 847, 956
XceI RCATGY 1 cut(s) 551
XmiI GTMKAC 1 cut(s) 921
XmnI GAANNNNTTC 1 cut(s) 1224
XspI CTAG 6 cut(s) 57, 105, 110, 183, 195, 390
Zsp2I ATGCAT 1 cut(s) 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.