Rh5DG163700

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0012861
Physical Location & Seq
Reverse (-)
17120198 .. 17125412
5215 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG163700.1

Sequence Viewer

Length: 993 bp
ATGTGCAGCTCTAAGGCCAAGGTGACCATGGGGATTGAAATCACACCCCTTGTTTCTAGAATCAACGGCAGACCAGTGCTTCAGCCTACCTGCAATCGAGTTCCTAGCCTAGACCGGCGCAATTCCCTTAAGAAACTGTCAACAGCACCACCACCTCTACCATTATCAACTTCTTCTTCTACCAGCACTAGTCCTAGAATTAGTACCAAGGCTTCATTGACAACACCACCAATTTCTCCAAAGTCAAAATCTCCGAGGCCACCTGCGATAAAGAGATCAGGCAATGACCCTAATGGCTTGAACTCCAGTTCTGAGAAAGTTGTGACACCAGGAGGTACCACAAGAGCTAAGGTTTTGGAGAGGAAGAAGTCAAAGAGTTTTAAGCTTGGTGTTGGTGCTGATAATGCTCATGATCATGGTAGACTCTCTTCTGCTTCGATTGAGGCTTCGTTGAGCTACTCCTCTTCTCTGATCACCGAGGCACCAGGAACCATTGCAGCTGGGAGGAGGGAGCAGATGGCTCTTCAACATGCACAGAGAAAGATGAGGATTGCTCATTATGGAAGATCAAAGTCTGCAAATTTTGAAAGGGTTGCTCCAGTTGATACTATGGAAGCCAAAGCTGGTGAAGAAGATCACAAAAGATGCAGCTTCATCACGGCTAATTCAGATCCCATATATGTTGCTTACCATGATCAAGAATGGGGAGTTCCTGTTCATGATGACAAGATGTTATTTGAACTGCTTGTTCTAAGTGGAGCTCAAGTTGGATCAGATTGGACTTCCATTTTAAAGAAACGCCAAGATTTCAGGGATGCATTTTCTGGGTTTGATGCTGAAGCTGTGGCCAGTCTCACTGACAAGCAAATGATTTCGATTTGTTCAGAATATGGCATTGATATAAGCAGAGTTAGAGGAGTAGTAGACAACTCTAACAGAATTCTTGAGGTAATACCAAGCATCAAATTCTGCAATTTTTTGTTTGTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

330

Amino Acids

35.9

Weight (kDa)

9.66

Isoelectric Point (pI)

44.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 221 - 327 2.7e-30 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012861)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12710
fragaria_vesca FvH4_3g13890
malus_domestica MD05G1240400.v1.1 MD10G1218500.v1.1
prunus_persica Prupe.4G125700_v2.0.a1
pyrus_communis pycom05g21640 pycom10g18430
rosa_chinensis RchiOBHm_Chr5g0023071
rosa_laevigata RLG00000032698
rosa_multiflora Rmu_co8421393.1_g000001
rosa_roxburghii Rroxscaffold_1G00056250
rosa_rugosa Rorug05G0077100
rosa_samantha Rh5AG163200 Rh5BG162500 Rh5CG177300 Rh5DG163700
rosa_wichuraiana Rw5G014720 Rw5G014990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 271
Acc36I ACCTGC 2 cut(s) 98, 271
Acc65I GGTACC 1 cut(s) 335
AccB1I GGYRCC 2 cut(s) 335, 481
AccI GTMKAC 2 cut(s) 421, 924
AclWI GGATC 2 cut(s) 665, 778
AcoI YGGCCR 1 cut(s) 846
AcsI RAATTY 3 cut(s) 580, 939, 965
AcuI CTGAAG 2 cut(s) 65, 858
AfaI GTAC 2 cut(s) 205, 337
AflII CTTAAG 1 cut(s) 128
AgsI TTSAA 5 cut(s) 38, 301, 527, 587, 740
AhlI ACTAGT 1 cut(s) 188
AjnI CCWGG 2 cut(s) 328, 484
AloI GAACNNNNNNTCC 2 cut(s) 699, 731
AluBI AGCT 9 cut(s) 9, 347, 385, 456, 500, 623, 651, 761, 842
AluI AGCT 9 cut(s) 9, 347, 385, 456, 500, 623, 651, 761, 842
Alw21I GWGCWC 1 cut(s) 763
Alw26I GTCTC 1 cut(s) 857
AlwI GGATC 2 cut(s) 665, 778
AoxI GGCC 3 cut(s) 15, 257, 846
ApeKI GCWGC 3 cut(s) 6, 497, 648
ApoI RAATTY 3 cut(s) 580, 939, 965
Asp718I GGTACC 1 cut(s) 335
AspLEI GCGC 1 cut(s) 120
AsuHPI GGTGA 3 cut(s) 34, 466, 638
BalI TGGCCA 1 cut(s) 848
BanI GGYRCC 2 cut(s) 335, 481
BanII GRGCYC 1 cut(s) 763
BarI GAAGNNNNNNTAC 4 cut(s) 196, 228, 412, 444
BbsI GAAGAC 1 cut(s) 979
Bbv12I GWGCWC 1 cut(s) 763
BbvI GCAGC 3 cut(s) 18, 509, 660
BccI CCATC 1 cut(s) 511
BceAI ACGGC 2 cut(s) 82, 675
BciT130I CCWGG 2 cut(s) 330, 486
BclI TGATCA 3 cut(s) 412, 471, 694
BcoDI GTCTC 1 cut(s) 857
BcuI ACTAGT 1 cut(s) 188
BfaI CTAG 5 cut(s) 57, 105, 110, 189, 195
BfrI CTTAAG 1 cut(s) 128
BfuAI ACCTGC 2 cut(s) 98, 271
BisI GCNGC 3 cut(s) 7, 498, 649
BlsI GCNGC 3 cut(s) 8, 499, 650
Bme1390I CCNGG 2 cut(s) 330, 486
BmiI GGNNCC 3 cut(s) 337, 483, 490
BmrFI CCNGG 2 cut(s) 330, 486
BmsI GCATC 4 cut(s) 635, 805, 823, 969
BpiI GAAGAC 1 cut(s) 979
BplI GAGNNNNNCTC 2 cut(s) 538, 570
BpmI CTGGAG 2 cut(s) 289, 582
Bpu10I CCTNAGC 1 cut(s) 348
BpuEI CTTGAG 2 cut(s) 747, 965
BsaBI GATNNNNATC 1 cut(s) 38
BsaJI CCNNGG 5 cut(s) 18, 27, 207, 254, 477
BsaXI ACNNNNNCTCC 4 cut(s) 699, 729, 750, 780
Bse118I RCCGGY 1 cut(s) 114
Bse1I ACTGG 4 cut(s) 74, 306, 599, 849
Bse3DI GCAATG 2 cut(s) 289, 492
Bse8I GATNNNNATC 1 cut(s) 38
BseBI CCWGG 2 cut(s) 330, 486
BseDI CCNNGG 5 cut(s) 18, 27, 207, 254, 477
BseGI GGATG 1 cut(s) 820
BseJI GATNNNNATC 1 cut(s) 38
BseMI GCAATG 2 cut(s) 289, 492
BseMII CTCAG 1 cut(s) 303
BseNI ACTGG 4 cut(s) 74, 306, 599, 849
BseRI GAGGAG 3 cut(s) 451, 520, 930
BseXI GCAGC 3 cut(s) 18, 509, 660
BseYI CCCAGC 1 cut(s) 500
BsgI GTGCAG 1 cut(s) 25
BshFI GGCC 3 cut(s) 17, 259, 848
BshNI GGYRCC 2 cut(s) 335, 481
BsiHKAI GWGCWC 1 cut(s) 763
BsiSI CCGG 1 cut(s) 115
BsmAI GTCTC 1 cut(s) 857
BsnI GGCC 3 cut(s) 17, 259, 848
Bsp1286I GDGCHC 1 cut(s) 763
Bsp143I GATC 8 cut(s) 275, 412, 471, 566, 634, 670, 694, 770
Bsp19I CCATGG 1 cut(s) 27
BspANI GGCC 3 cut(s) 17, 259, 848
BspCNI CTCAG 1 cut(s) 304
BspHI TCATGA 2 cut(s) 409, 718
BspLI GGNNCC 3 cut(s) 337, 483, 490
BspMI ACCTGC 2 cut(s) 98, 271
BspPI GGATC 2 cut(s) 665, 778
BspQI GCTCTTC 1 cut(s) 528
BspT107I GGYRCC 2 cut(s) 335, 481
BspTI CTTAAG 1 cut(s) 128
BsrDI GCAATG 2 cut(s) 289, 492
BsrFI RCCGGY 1 cut(s) 114
BsrI ACTGG 4 cut(s) 74, 306, 599, 849
BssAI RCCGGY 1 cut(s) 114
BssECI CCNNGG 5 cut(s) 18, 27, 207, 254, 477
BssMI GATC 8 cut(s) 275, 412, 471, 566, 634, 670, 694, 770
BssT1I CCWWGG 3 cut(s) 18, 27, 207
Bst2UI CCWGG 2 cut(s) 330, 486
Bst4CI ACNGT 1 cut(s) 138
Bst6I CTCTTC 3 cut(s) 433, 469, 528
BstAFI CTTAAG 1 cut(s) 128
BstDEI CTNAG 4 cut(s) 12, 312, 348, 752
BstDSI CCRYGG 1 cut(s) 27
BstEII GGTNACC 1 cut(s) 22
BstF5I GGATG 1 cut(s) 820
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 8 cut(s) 278, 415, 474, 569, 637, 673, 697, 773
BstMAI GTCTC 1 cut(s) 857
BstMBI GATC 8 cut(s) 275, 412, 471, 566, 634, 670, 694, 770
BstMWI GCNNNNNNNGC 1 cut(s) 404
BstNI CCWGG 2 cut(s) 330, 486
BstNSI RCATGY 1 cut(s) 533
BstPI GGTNACC 1 cut(s) 22
BstSCI CCNGG 2 cut(s) 328, 484
BstV1I GCAGC 3 cut(s) 18, 509, 660
BstV2I GAAGAC 1 cut(s) 979
BstX2I RGATCY 1 cut(s) 670
BstYI RGATCY 1 cut(s) 670
BsuRI GGCC 3 cut(s) 17, 259, 848
BtgI CCRYGG 1 cut(s) 27
BtsCI GGATG 1 cut(s) 820
BtsIMutI CAGTG 2 cut(s) 81, 855
BveI ACCTGC 2 cut(s) 98, 271
CciI TCATGA 2 cut(s) 409, 718
CfoI GCGC 1 cut(s) 120
Cfr10I RCCGGY 1 cut(s) 114
Csp6I GTAC 2 cut(s) 204, 336
CviAII CATG 6 cut(s) 28, 410, 416, 530, 692, 719
CviQI GTAC 2 cut(s) 204, 336
DdeI CTNAG 4 cut(s) 12, 312, 348, 752
DpnI GATC 8 cut(s) 277, 414, 473, 568, 636, 672, 696, 772
DpnII GATC 8 cut(s) 275, 412, 471, 566, 634, 670, 694, 770
DraI TTTAAA 1 cut(s) 792
EaeI YGGCCR 1 cut(s) 846
Eam1104I CTCTTC 3 cut(s) 433, 469, 528
EarI CTCTTC 3 cut(s) 433, 469, 528
Ecl136II GAGCTC 1 cut(s) 761
Eco130I CCWWGG 3 cut(s) 18, 27, 207
Eco24I GRGCYC 1 cut(s) 763
Eco53kI GAGCTC 1 cut(s) 761
Eco57I CTGAAG 2 cut(s) 65, 858
Eco91I GGTNACC 1 cut(s) 22
EcoICRI GAGCTC 1 cut(s) 761
EcoO65I GGTNACC 1 cut(s) 22
EcoRI GAATTC 1 cut(s) 939
EcoRII CCWGG 2 cut(s) 328, 484
EcoT14I CCWWGG 3 cut(s) 18, 27, 207
EcoT22I ATGCAT 1 cut(s) 820
EcoT38I GRGCYC 1 cut(s) 763
ErhI CCWWGG 3 cut(s) 18, 27, 207
FaeI CATG 6 cut(s) 31, 413, 419, 533, 695, 722
FatI CATG 6 cut(s) 27, 409, 415, 529, 691, 718
FbaI TGATCA 3 cut(s) 412, 471, 694
FblI GTMKAC 2 cut(s) 421, 924
Fnu4HI GCNGC 3 cut(s) 7, 498, 649
FokI GGATG 1 cut(s) 827
FriOI GRGCYC 1 cut(s) 763
Fsp4HI GCNGC 3 cut(s) 7, 498, 649
FspBI CTAG 5 cut(s) 57, 105, 110, 189, 195
GlaI GCGC 1 cut(s) 119
GluI GCNGC 3 cut(s) 7, 498, 649
GsaI CCCAGC 1 cut(s) 504
GsuI CTGGAG 2 cut(s) 289, 582
HaeIII GGCC 3 cut(s) 17, 259, 848
HapII CCGG 1 cut(s) 115
HhaI GCGC 1 cut(s) 120
Hin1II CATG 6 cut(s) 31, 413, 419, 533, 695, 722
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HincII GTYRAC 1 cut(s) 141
HindII GTYRAC 1 cut(s) 141
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 2 cut(s) 60, 423
HpaII CCGG 1 cut(s) 115
HphI GGTGA 3 cut(s) 34, 466, 638
Hpy166II GTNNAC 3 cut(s) 141, 422, 925
Hpy188I TCNGA 6 cut(s) 255, 313, 471, 670, 775, 886
Hpy188III TCNNGA 5 cut(s) 57, 410, 698, 719, 944
Hpy8I GTNNAC 3 cut(s) 141, 422, 925
HpyCH4III ACNGT 1 cut(s) 138
HpyCH4V TGCA 8 cut(s) 6, 93, 497, 533, 578, 648, 818, 972
HpyF10VI GCNNNNNNNGC 1 cut(s) 404
HpyF3I CTNAG 4 cut(s) 12, 312, 348, 752
Hsp92II CATG 6 cut(s) 31, 413, 419, 533, 695, 722
HspAI GCGC 1 cut(s) 118
KpnI GGTACC 1 cut(s) 339
Ksp22I TGATCA 3 cut(s) 412, 471, 694
Kzo9I GATC 8 cut(s) 275, 412, 471, 566, 634, 670, 694, 770
LguI GCTCTTC 1 cut(s) 528
LmnI GCTCC 3 cut(s) 511, 601, 758
Lsp1109I GCAGC 3 cut(s) 18, 509, 660
LweI GCATC 4 cut(s) 635, 805, 823, 969
MaeI CTAG 5 cut(s) 57, 105, 110, 189, 195
MaeIII GTNAC 2 cut(s) 22, 322
MalI GATC 8 cut(s) 277, 414, 473, 568, 636, 672, 696, 772
MboI GATC 8 cut(s) 275, 412, 471, 566, 634, 670, 694, 770
MflI RGATCY 1 cut(s) 670
MhlI GDGCHC 1 cut(s) 763
MlsI TGGCCA 1 cut(s) 848
MluCI AATT 8 cut(s) 121, 198, 231, 580, 664, 939, 965, 973
MluNI TGGCCA 1 cut(s) 848
MlyI GAGTC 1 cut(s) 417
MmeI TCCRAC 1 cut(s) 748
Mox20I TGGCCA 1 cut(s) 848
Mph1103I ATGCAT 1 cut(s) 820
MscI TGGCCA 1 cut(s) 848
MseI TTAA 3 cut(s) 129, 381, 791
MslI CAYNNNNRTG 1 cut(s) 414
Msp20I TGGCCA 1 cut(s) 848
MspA1I CMGCKG 1 cut(s) 500
MspCI CTTAAG 1 cut(s) 128
MspI CCGG 1 cut(s) 115
MspR9I CCNGG 2 cut(s) 330, 486
MvaI CCWGG 2 cut(s) 330, 486
MwoI GCNNNNNNNGC 1 cut(s) 404
NcoI CCATGG 1 cut(s) 27
NdeII GATC 8 cut(s) 275, 412, 471, 566, 634, 670, 694, 770
NlaIII CATG 6 cut(s) 31, 413, 419, 533, 695, 722
NlaIV GGNNCC 3 cut(s) 337, 483, 490
NmuCI GTSAC 2 cut(s) 22, 322
NsiI ATGCAT 1 cut(s) 820
NspI RCATGY 1 cut(s) 533
PagI TCATGA 2 cut(s) 409, 718
PaqCI CACCTGC 1 cut(s) 271
PciSI GCTCTTC 1 cut(s) 528
PfeI GAWTC 1 cut(s) 60
PkrI GCNGC 3 cut(s) 8, 499, 650
PleI GAGTC 1 cut(s) 417
PpsI GAGTC 1 cut(s) 417
Psp124BI GAGCTC 1 cut(s) 763
Psp6I CCWGG 2 cut(s) 328, 484
PspEI GGTNACC 1 cut(s) 22
PspFI CCCAGC 1 cut(s) 500
PspGI CCWGG 2 cut(s) 328, 484
PspN4I GGNNCC 3 cut(s) 337, 483, 490
PsuI RGATCY 1 cut(s) 670
PvuII CAGCTG 1 cut(s) 500
RsaI GTAC 2 cut(s) 205, 337
RsaNI GTAC 2 cut(s) 204, 336
RseI CAYNNNNRTG 1 cut(s) 414
SacI GAGCTC 1 cut(s) 763
SapI GCTCTTC 1 cut(s) 528
SaqAI TTAA 3 cut(s) 129, 381, 791
SatI GCNGC 3 cut(s) 7, 498, 649
Sau3AI GATC 8 cut(s) 275, 412, 471, 566, 634, 670, 694, 770
SchI GAGTC 1 cut(s) 417
ScrFI CCNGG 2 cut(s) 330, 486
SduI GDGCHC 1 cut(s) 763
SfaNI GCATC 4 cut(s) 635, 805, 823, 969
SmiMI CAYNNNNRTG 1 cut(s) 414
SmlI CTYRAG 3 cut(s) 128, 762, 944
SmoI CTYRAG 3 cut(s) 128, 762, 944
SpeI ACTAGT 1 cut(s) 188
Sse9I AATT 8 cut(s) 121, 198, 231, 580, 664, 939, 965, 973
SspMI CTAG 5 cut(s) 57, 105, 110, 189, 195
SstI GAGCTC 1 cut(s) 763
StyD4I CCNGG 2 cut(s) 328, 484
StyI CCWWGG 3 cut(s) 18, 27, 207
TaaI ACNGT 1 cut(s) 138
TaqI TCGA 3 cut(s) 97, 437, 875
TasI AATT 8 cut(s) 121, 198, 231, 580, 664, 939, 965, 973
TfiI GAWTC 1 cut(s) 60
Tru1I TTAA 3 cut(s) 129, 381, 791
Tru9I TTAA 3 cut(s) 129, 381, 791
TscAI CASTG 2 cut(s) 81, 862
TseFI GTSAC 2 cut(s) 22, 322
TseI GCWGC 3 cut(s) 6, 497, 648
Tsp45I GTSAC 2 cut(s) 22, 322
TspDTI ATGAA 3 cut(s) 204, 643, 707
TspRI CASTG 2 cut(s) 81, 862
Vha464I CTTAAG 1 cut(s) 128
XapI RAATTY 3 cut(s) 580, 939, 965
XbaI TCTAGA 1 cut(s) 56
XceI RCATGY 1 cut(s) 533
XcmI CCANNNNNNNNNTGG 1 cut(s) 25
XmiI GTMKAC 2 cut(s) 421, 924
XspI CTAG 5 cut(s) 57, 105, 110, 189, 195
Zsp2I ATGCAT 1 cut(s) 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.