Rw5G014990

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0012861
Physical Location & Seq
Reverse (-)
18046089 .. 18048029
1941 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G014990.1

Sequence Viewer

Length: 1230 bp
ATGTGCAGCTCTAAGGCCAAGGTGACCATGGGGATTGAAATCACACCCCTTGTTTCTAGAATCAATGGCAGACCAGTGCTTCAGCCTACCTGCAATCGAAAACTGTCAACAGCACCACCACCTCTACCATTATCAACTTCTTCTACCAGCACTAGTCCTAGAATTAGTACCAAGGCTTCATTGACAACACCACCAATTTCTCCAAAGTCAAAATCTCCGAGGCCACCTGCGATAAAGAGATCAGGCAATGACCCTAATGGCTTGAACTCCAGTTCTGAGAAAGTTGTGACACCAGGAGGTACCACAAGAGCTAAGGTTTTGGAAAGGAAGAAGTCAAAGAGTTTTAAGCTTGGTGTTGGTGCTGATAATGCTCATGATCATGGTAGACTCTCTTCTGCTTCGATTGAGGCTTCGTTGAGCTACTCCTCTTCTCTGATCACCGAGGCACCAGGAACCATTGCAGCTGGGAGGAGGGAGCAGATGGCTCTTCAACATGCACAGAGAAAGATGAGGATTGCTCATTATGGAAGATCAAAGTCTGCAAATTTTGAAAGGGTTGCTCCAGTTGATACTATGGAAGCCAAAGCTGGTGAAGAAGATCACAAAAGATGCAGCTTCATCACGGCTAATTCAGATCCCATTTATGTTGCTTACCATGATCAAGAATGGGGAGTTCCTGTTCATGATGACAAGATGTTATTTGAACTGCTTGTTCTAAGTGGAGCTCAAGTTGGATCAGATTGGACTTCCATTTTAAAGAAACCTGTGGCCAGTCTCACTGACAAGCAAATGATTTCGATTTGTTCAGAATATGGCATTGATATAAGCAGAGTTAGAGGAGTAGTAGACAACTCTAACAGAATTCTTGAGGTAAAGAAGGAATTTGGGTCATTTCACAAATACATATGGGGGTTTGTGAATCACAAGCCAATTTCCCCTCAATACAAGCAAGGCTACAAAATCCCAGTGAAGACATCAAAATCAGAGAGCATAAGCAAAGACATGGTTAGGAGGGGGTTTAGGTTTGTTGGTCCAACAGTTGTTCATTCCTTCATGCAAGCCTCAGGCCTCACAAATGACCATTTGACCACCTGCCACAGGCCTTCAGTGCACCTTACTAGCAGCCCATCCTACCACATTGGAACAAGCTCTAGGATTATTGACCCCAGAAGAGAAAAATTCATGGACAAAGTTAATCGTCCAAAAAAATATATATATGTTCAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

409

Amino Acids

45.03

Weight (kDa)

9.95

Isoelectric Point (pI)

44.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 209 - 367 3.7e-50 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012861)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12710
fragaria_vesca FvH4_3g13890
malus_domestica MD05G1240400.v1.1 MD10G1218500.v1.1
prunus_persica Prupe.4G125700_v2.0.a1
pyrus_communis pycom05g21640 pycom10g18430
rosa_chinensis RchiOBHm_Chr5g0023071
rosa_laevigata RLG00000032698
rosa_multiflora Rmu_co8421393.1_g000001
rosa_roxburghii Rroxscaffold_1G00056250
rosa_rugosa Rorug05G0077100
rosa_samantha Rh5AG163200 Rh5BG162500 Rh5CG177300 Rh5DG163700
rosa_wichuraiana Rw5G014720 Rw5G014990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 235, 1100
Acc36I ACCTGC 3 cut(s) 98, 235, 1100
Acc65I GGTACC 1 cut(s) 299
AccB1I GGYRCC 2 cut(s) 299, 445
AccI GTMKAC 2 cut(s) 385, 846
AclWI GGATC 2 cut(s) 629, 742
AcoI YGGCCR 1 cut(s) 768
AcsI RAATTY 4 cut(s) 544, 861, 881, 1178
AcuI CTGAAG 2 cut(s) 65, 1089
AfaI GTAC 2 cut(s) 169, 301
AfiI CCNNNNNNNGG 1 cut(s) 1098
AgsI TTSAA 6 cut(s) 38, 265, 491, 551, 704, 1223
AhlI ACTAGT 1 cut(s) 152
AjnI CCWGG 2 cut(s) 292, 448
AloI GAACNNNNNNTCC 2 cut(s) 663, 695
AluBI AGCT 9 cut(s) 9, 311, 349, 420, 464, 587, 615, 725, 1149
AluI AGCT 9 cut(s) 9, 311, 349, 420, 464, 587, 615, 725, 1149
Alw21I GWGCWC 2 cut(s) 727, 1113
Alw26I GTCTC 1 cut(s) 779
Alw44I GTGCAC 1 cut(s) 1109
AlwI GGATC 2 cut(s) 629, 742
AoxI GGCC 5 cut(s) 15, 221, 768, 1066, 1100
ApaLI GTGCAC 1 cut(s) 1109
ApeKI GCWGC 4 cut(s) 6, 461, 612, 1122
ApoI RAATTY 4 cut(s) 544, 861, 881, 1178
Asp718I GGTACC 1 cut(s) 299
AspS9I GGNCC 1 cut(s) 1031
AsuHPI GGTGA 3 cut(s) 34, 430, 602
AvaII GGWCC 1 cut(s) 1031
AxyI CCTNAGG 1 cut(s) 1063
BaeGI GKGCMC 1 cut(s) 1113
BalI TGGCCA 1 cut(s) 770
BanI GGYRCC 2 cut(s) 299, 445
BanII GRGCYC 1 cut(s) 727
BarI GAAGNNNNNNTAC 4 cut(s) 160, 192, 376, 408
BbsI GAAGAC 1 cut(s) 977
Bbv12I GWGCWC 2 cut(s) 727, 1113
BbvI GCAGC 4 cut(s) 18, 473, 624, 1134
BccI CCATC 2 cut(s) 475, 1135
BceAI ACGGC 1 cut(s) 639
BciT130I CCWGG 2 cut(s) 294, 450
BclI TGATCA 3 cut(s) 376, 435, 658
BcoDI GTCTC 1 cut(s) 779
BcuI ACTAGT 1 cut(s) 152
BfaI CTAG 5 cut(s) 57, 153, 159, 1119, 1152
BfuAI ACCTGC 3 cut(s) 98, 235, 1100
BisI GCNGC 4 cut(s) 7, 462, 613, 1123
BlsI GCNGC 4 cut(s) 8, 463, 614, 1124
Bme1390I CCNGG 2 cut(s) 294, 450
Bme18I GGWCC 1 cut(s) 1031
BmgT120I GGNCC 1 cut(s) 1031
BmiI GGNNCC 3 cut(s) 301, 447, 454
BmrFI CCNGG 2 cut(s) 294, 450
BmrI ACTGGG 1 cut(s) 959
BmsI GCATC 1 cut(s) 599
BmuI ACTGGG 1 cut(s) 959
BpiI GAAGAC 1 cut(s) 977
BplI GAGNNNNNCTC 2 cut(s) 502, 534
BpmI CTGGAG 2 cut(s) 253, 546
Bpu10I CCTNAGC 1 cut(s) 312
BpuEI CTTGAG 2 cut(s) 711, 887
BsaBI GATNNNNATC 1 cut(s) 38
BsaJI CCNNGG 5 cut(s) 18, 27, 171, 218, 441
BsaXI ACNNNNNCTCC 4 cut(s) 663, 693, 714, 744
Bsc4I CCNNNNNNNGG 1 cut(s) 1098
Bse1I ACTGG 5 cut(s) 74, 270, 563, 771, 965
Bse21I CCTNAGG 1 cut(s) 1063
Bse3DI GCAATG 2 cut(s) 253, 456
Bse8I GATNNNNATC 1 cut(s) 38
BseBI CCWGG 2 cut(s) 294, 450
BseDI CCNNGG 5 cut(s) 18, 27, 171, 218, 441
BseGI GGATG 1 cut(s) 1127
BseJI GATNNNNATC 1 cut(s) 38
BseLI CCNNNNNNNGG 1 cut(s) 1098
BseMI GCAATG 2 cut(s) 253, 456
BseMII CTCAG 2 cut(s) 267, 1077
BseNI ACTGG 5 cut(s) 74, 270, 563, 771, 965
BseRI GAGGAG 3 cut(s) 415, 484, 852
BseSI GKGCMC 1 cut(s) 1113
BseXI GCAGC 4 cut(s) 18, 473, 624, 1134
BseYI CCCAGC 1 cut(s) 464
BsgI GTGCAG 1 cut(s) 25
BshFI GGCC 5 cut(s) 17, 223, 770, 1068, 1102
BshNI GGYRCC 2 cut(s) 299, 445
BsiHKAI GWGCWC 2 cut(s) 727, 1113
BslI CCNNNNNNNGG 1 cut(s) 1098
BsmAI GTCTC 1 cut(s) 779
BsnI GGCC 5 cut(s) 17, 223, 770, 1068, 1102
Bsp1286I GDGCHC 2 cut(s) 727, 1113
Bsp143I GATC 8 cut(s) 239, 376, 435, 530, 598, 634, 658, 734
Bsp19I CCATGG 1 cut(s) 27
BspANI GGCC 5 cut(s) 17, 223, 770, 1068, 1102
BspCNI CTCAG 2 cut(s) 268, 1076
BspHI TCATGA 2 cut(s) 373, 682
BspLI GGNNCC 3 cut(s) 301, 447, 454
BspMI ACCTGC 3 cut(s) 98, 235, 1100
BspPI GGATC 2 cut(s) 629, 742
BspQI GCTCTTC 1 cut(s) 492
BspT107I GGYRCC 2 cut(s) 299, 445
BsrDI GCAATG 2 cut(s) 253, 456
BsrI ACTGG 5 cut(s) 74, 270, 563, 771, 965
BssECI CCNNGG 5 cut(s) 18, 27, 171, 218, 441
BssMI GATC 8 cut(s) 239, 376, 435, 530, 598, 634, 658, 734
BssT1I CCWWGG 3 cut(s) 18, 27, 171
Bst2UI CCWGG 2 cut(s) 294, 450
Bst4CI ACNGT 2 cut(s) 105, 1039
Bst6I CTCTTC 4 cut(s) 397, 433, 492, 1165
BstC8I GCNNGC 1 cut(s) 1059
BstDEI CTNAG 5 cut(s) 12, 276, 312, 716, 1063
BstDSI CCRYGG 1 cut(s) 27
BstEII GGTNACC 1 cut(s) 22
BstENI CCTNNNNNAGG 1 cut(s) 1096
BstF5I GGATG 1 cut(s) 1127
BstKTI GATC 8 cut(s) 242, 379, 438, 533, 601, 637, 661, 737
BstMAI GTCTC 1 cut(s) 779
BstMBI GATC 8 cut(s) 239, 376, 435, 530, 598, 634, 658, 734
BstMWI GCNNNNNNNGC 2 cut(s) 368, 1108
BstNI CCWGG 2 cut(s) 294, 450
BstNSI RCATGY 1 cut(s) 497
BstPI GGTNACC 1 cut(s) 22
BstSCI CCNGG 2 cut(s) 292, 448
BstSLI GKGCMC 1 cut(s) 1113
BstV1I GCAGC 4 cut(s) 18, 473, 624, 1134
BstV2I GAAGAC 1 cut(s) 977
BstX2I RGATCY 1 cut(s) 634
BstYI RGATCY 1 cut(s) 634
Bsu36I CCTNAGG 1 cut(s) 1063
BsuRI GGCC 5 cut(s) 17, 223, 770, 1068, 1102
BtgI CCRYGG 1 cut(s) 27
BtsCI GGATG 1 cut(s) 1127
BtsIMutI CAGTG 4 cut(s) 81, 777, 972, 1113
BveI ACCTGC 3 cut(s) 98, 235, 1100
Cac8I GCNNGC 1 cut(s) 1059
CciI TCATGA 2 cut(s) 373, 682
Cfr13I GGNCC 1 cut(s) 1031
Csp6I GTAC 2 cut(s) 168, 300
CviQI GTAC 2 cut(s) 168, 300
DdeI CTNAG 5 cut(s) 12, 276, 312, 716, 1063
DpnI GATC 8 cut(s) 241, 378, 437, 532, 600, 636, 660, 736
DpnII GATC 8 cut(s) 239, 376, 435, 530, 598, 634, 658, 734
DraI TTTAAA 1 cut(s) 756
EaeI YGGCCR 1 cut(s) 768
Eam1104I CTCTTC 4 cut(s) 397, 433, 492, 1165
EarI CTCTTC 4 cut(s) 397, 433, 492, 1165
Ecl136II GAGCTC 1 cut(s) 725
Eco130I CCWWGG 3 cut(s) 18, 27, 171
Eco147I AGGCCT 2 cut(s) 1068, 1102
Eco24I GRGCYC 1 cut(s) 727
Eco47I GGWCC 1 cut(s) 1031
Eco53kI GAGCTC 1 cut(s) 725
Eco57I CTGAAG 2 cut(s) 65, 1089
Eco81I CCTNAGG 1 cut(s) 1063
Eco91I GGTNACC 1 cut(s) 22
EcoICRI GAGCTC 1 cut(s) 725
EcoNI CCTNNNNNAGG 1 cut(s) 1096
EcoO65I GGTNACC 1 cut(s) 22
EcoRI GAATTC 1 cut(s) 861
EcoRII CCWGG 2 cut(s) 292, 448
EcoT14I CCWWGG 3 cut(s) 18, 27, 171
EcoT38I GRGCYC 1 cut(s) 727
ErhI CCWWGG 3 cut(s) 18, 27, 171
FauNDI CATATG 1 cut(s) 905
FbaI TGATCA 3 cut(s) 376, 435, 658
FblI GTMKAC 2 cut(s) 385, 846
Fnu4HI GCNGC 4 cut(s) 7, 462, 613, 1123
FokI GGATG 1 cut(s) 1114
FriOI GRGCYC 1 cut(s) 727
Fsp4HI GCNGC 4 cut(s) 7, 462, 613, 1123
FspBI CTAG 5 cut(s) 57, 153, 159, 1119, 1152
GluI GCNGC 4 cut(s) 7, 462, 613, 1123
GsaI CCCAGC 1 cut(s) 468
GsuI CTGGAG 2 cut(s) 253, 546
HaeIII GGCC 5 cut(s) 17, 223, 770, 1068, 1102
HincII GTYRAC 1 cut(s) 108
HindII GTYRAC 1 cut(s) 108
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 3 cut(s) 60, 387, 919
HphI GGTGA 3 cut(s) 34, 430, 602
Hpy166II GTNNAC 4 cut(s) 108, 386, 847, 1111
Hpy188I TCNGA 7 cut(s) 219, 277, 435, 634, 739, 808, 985
Hpy188III TCNNGA 5 cut(s) 57, 374, 662, 683, 866
Hpy8I GTNNAC 4 cut(s) 108, 386, 847, 1111
HpyAV CCTTC 3 cut(s) 871, 1060, 1113
HpyCH4III ACNGT 2 cut(s) 105, 1039
HpyCH4V TGCA 8 cut(s) 6, 93, 461, 497, 542, 612, 1057, 1111
HpyF10VI GCNNNNNNNGC 2 cut(s) 368, 1108
HpyF3I CTNAG 5 cut(s) 12, 276, 312, 716, 1063
KpnI GGTACC 1 cut(s) 303
Ksp22I TGATCA 3 cut(s) 376, 435, 658
Kzo9I GATC 8 cut(s) 239, 376, 435, 530, 598, 634, 658, 734
LguI GCTCTTC 1 cut(s) 492
LmnI GCTCC 3 cut(s) 475, 565, 722
Lsp1109I GCAGC 4 cut(s) 18, 473, 624, 1134
LweI GCATC 1 cut(s) 599
MaeI CTAG 5 cut(s) 57, 153, 159, 1119, 1152
MaeIII GTNAC 2 cut(s) 22, 286
MalI GATC 8 cut(s) 241, 378, 437, 532, 600, 636, 660, 736
MboI GATC 8 cut(s) 239, 376, 435, 530, 598, 634, 658, 734
MflI RGATCY 1 cut(s) 634
MhlI GDGCHC 2 cut(s) 727, 1113
MlsI TGGCCA 1 cut(s) 770
MluCI AATT 8 cut(s) 162, 195, 544, 628, 861, 881, 930, 1178
MluNI TGGCCA 1 cut(s) 770
MlyI GAGTC 1 cut(s) 381
MmeI TCCRAC 2 cut(s) 712, 1058
Mox20I TGGCCA 1 cut(s) 770
MscI TGGCCA 1 cut(s) 770
MseI TTAA 3 cut(s) 345, 755, 1194
MslI CAYNNNNRTG 1 cut(s) 378
Msp20I TGGCCA 1 cut(s) 770
MspA1I CMGCKG 1 cut(s) 464
MspR9I CCNGG 2 cut(s) 294, 450
MvaI CCWGG 2 cut(s) 294, 450
MwoI GCNNNNNNNGC 2 cut(s) 368, 1108
NcoI CCATGG 1 cut(s) 27
NdeI CATATG 1 cut(s) 905
NdeII GATC 8 cut(s) 239, 376, 435, 530, 598, 634, 658, 734
NlaIV GGNNCC 3 cut(s) 301, 447, 454
NmuCI GTSAC 2 cut(s) 22, 286
NspI RCATGY 1 cut(s) 497
PagI TCATGA 2 cut(s) 373, 682
PaqCI CACCTGC 2 cut(s) 235, 1100
PceI AGGCCT 2 cut(s) 1068, 1102
PciSI GCTCTTC 1 cut(s) 492
PfeI GAWTC 2 cut(s) 60, 919
PkrI GCNGC 4 cut(s) 8, 463, 614, 1124
PleI GAGTC 1 cut(s) 381
PpsI GAGTC 1 cut(s) 381
Psp124BI GAGCTC 1 cut(s) 727
Psp6I CCWGG 2 cut(s) 292, 448
PspEI GGTNACC 1 cut(s) 22
PspFI CCCAGC 1 cut(s) 464
PspGI CCWGG 2 cut(s) 292, 448
PspN4I GGNNCC 3 cut(s) 301, 447, 454
PspPI GGNCC 1 cut(s) 1031
PsuI RGATCY 1 cut(s) 634
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 2 cut(s) 169, 301
RsaNI GTAC 2 cut(s) 168, 300
RseI CAYNNNNRTG 1 cut(s) 378
SacI GAGCTC 1 cut(s) 727
SapI GCTCTTC 1 cut(s) 492
SaqAI TTAA 3 cut(s) 345, 755, 1194
SatI GCNGC 4 cut(s) 7, 462, 613, 1123
Sau3AI GATC 8 cut(s) 239, 376, 435, 530, 598, 634, 658, 734
Sau96I GGNCC 1 cut(s) 1031
SchI GAGTC 1 cut(s) 381
ScrFI CCNGG 2 cut(s) 294, 450
SduI GDGCHC 2 cut(s) 727, 1113
SfaNI GCATC 1 cut(s) 599
SinI GGWCC 1 cut(s) 1031
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 2 cut(s) 726, 866
SmoI CTYRAG 2 cut(s) 726, 866
SpeI ACTAGT 1 cut(s) 152
Sse9I AATT 8 cut(s) 162, 195, 544, 628, 861, 881, 930, 1178
SseBI AGGCCT 2 cut(s) 1068, 1102
SspMI CTAG 5 cut(s) 57, 153, 159, 1119, 1152
SstI GAGCTC 1 cut(s) 727
StuI AGGCCT 2 cut(s) 1068, 1102
StyD4I CCNGG 2 cut(s) 292, 448
StyI CCWWGG 3 cut(s) 18, 27, 171
TaaI ACNGT 2 cut(s) 105, 1039
TaqI TCGA 3 cut(s) 97, 401, 797
TasI AATT 8 cut(s) 162, 195, 544, 628, 861, 881, 930, 1178
TfiI GAWTC 2 cut(s) 60, 919
Tru1I TTAA 3 cut(s) 345, 755, 1194
Tru9I TTAA 3 cut(s) 345, 755, 1194
TscAI CASTG 4 cut(s) 81, 784, 972, 1113
TseFI GTSAC 2 cut(s) 22, 286
TseI GCWGC 4 cut(s) 6, 461, 612, 1122
Tsp45I GTSAC 2 cut(s) 22, 286
TspDTI ATGAA 6 cut(s) 168, 607, 671, 1034, 1042, 1171
TspRI CASTG 4 cut(s) 81, 784, 972, 1113
VneI GTGCAC 1 cut(s) 1109
VpaK11BI GGWCC 1 cut(s) 1031
XagI CCTNNNNNAGG 1 cut(s) 1096
XapI RAATTY 4 cut(s) 544, 861, 881, 1178
XbaI TCTAGA 1 cut(s) 56
XceI RCATGY 1 cut(s) 497
XcmI CCANNNNNNNNNTGG 1 cut(s) 25
XmiI GTMKAC 2 cut(s) 385, 846
XspI CTAG 5 cut(s) 57, 153, 159, 1119, 1152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.