Rh5BG162500

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0012861
Physical Location & Seq
Reverse (-)
16946282 .. 16948119
1838 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG162500.1

Sequence Viewer

Length: 1104 bp
ATGGGGATTGAAATCACACCCCTTGTTTCTAGAATCAATGGCAGACCAGTGCTTCAGCCTACCTGCAATCGAGTTCCTAGCCTAGACCGGCGCAATTCCCTTAAGAAACTGTCAACAGCACCACCACCTCTACCATTATCAACTTCTTCTTCTACCAGCACTAGTCCTAGAATTAGTACCAAGGCTTCATTGACAACACCACCAATTTCTCCAAAGTCAAAATCTCCGAGACCACCGGCGATAAAGAGATCAGGCAATGACCCTAATGGCTTGAACTCCAGTTCTGAGAAAGTAGTGACACCAGGAGGTACCACAAGAGCTAAGGTTTTGGAGAGGAAGAAGTCAAAGAGTTTTAAGCTTGGTGTTGGTGCTGATAATGCTCATGATCATGGTAGACTCTCTTCTGCTTCGATTGAGGCTTCGTTGAGCTATTCCTCTTCTCTGATCACCGAGGCACCAGGAACCATTGCAGCTGGGAGGAGGGAGCAGATGGCTCTTCAACATGCACAGAGAAAGATGAAGATTGCTCATTATGGAAGATCAAAGTCTGCAAATTTTGAAAGGGTTGCTCCAGTTGATACTATGGAAGCCAAAGCTGGTGAAGAAGATCACAAAAGATGCAGCTTCATCACGGCTAATTCAGATTGGACTTCCATTTTAAAGAAACGCCAAGATTTCAGGGATGCATTTTCTGGGTTTGATGCTGAAGCTGTGGCCAGTCTCACTGACAAGCAAATGATTTCGATTTGTTCAGAATATGGCATTGATATAAGCAGAGTTAGAGGAGTAGTAGACAACTCTAACAGAATTCTTGAGGTAAAGAAGGAATTTGGGTCATTTCACAAATACATATGGGGGTTTGTGAATCACAAGCCAATTTCCCCTCAATACAAGCAAGGCTACAAAATCCCAGTGAAGACATCAAAATCAGAGAGCATAAGCAAAGACATGGTTAGGAGGGGGTTTAGGTTTGTTGGTCCAACAGTTGTTCATTCCTTCATGCAAGCCTCAGGCCTCACAAATGACCATTTGACCACCTGCCACAGGCACCTTCAGTGCACCTTACTAGCAGCCCATCCTACCACATTGGAACAAGCTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

367

Amino Acids

40.18

Weight (kDa)

10.03

Isoelectric Point (pI)

47.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 211 - 350 9.2e-46 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012861)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12710
fragaria_vesca FvH4_3g13890
malus_domestica MD05G1240400.v1.1 MD10G1218500.v1.1
prunus_persica Prupe.4G125700_v2.0.a1
pyrus_communis pycom05g21640 pycom10g18430
rosa_chinensis RchiOBHm_Chr5g0023071
rosa_laevigata RLG00000032698
rosa_multiflora Rmu_co8421393.1_g000001
rosa_roxburghii Rroxscaffold_1G00056250
rosa_rugosa Rorug05G0077100
rosa_samantha Rh5AG163200 Rh5BG162500 Rh5CG177300 Rh5DG163700
rosa_wichuraiana Rw5G014720 Rw5G014990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1046
Acc36I ACCTGC 2 cut(s) 71, 1046
Acc65I GGTACC 1 cut(s) 308
AccB1I GGYRCC 3 cut(s) 308, 454, 1047
AccI GTMKAC 2 cut(s) 394, 792
AcoI YGGCCR 1 cut(s) 714
AcsI RAATTY 3 cut(s) 553, 807, 827
AcuI CTGAAG 3 cut(s) 38, 726, 1037
AfaI GTAC 2 cut(s) 178, 310
AfiI CCNNNNNNNGG 1 cut(s) 1044
AflII CTTAAG 1 cut(s) 101
AgsI TTSAA 4 cut(s) 11, 274, 500, 560
AhlI ACTAGT 1 cut(s) 161
AjnI CCWGG 2 cut(s) 301, 457
AluBI AGCT 8 cut(s) 320, 358, 429, 473, 596, 624, 710, 1097
AluI AGCT 8 cut(s) 320, 358, 429, 473, 596, 624, 710, 1097
Alw21I GWGCWC 1 cut(s) 1061
Alw26I GTCTC 2 cut(s) 223, 725
Alw44I GTGCAC 1 cut(s) 1057
AoxI GGCC 2 cut(s) 714, 1012
ApaLI GTGCAC 1 cut(s) 1057
ApeKI GCWGC 3 cut(s) 470, 621, 1070
ApoI RAATTY 3 cut(s) 553, 807, 827
Asp718I GGTACC 1 cut(s) 308
AspLEI GCGC 1 cut(s) 93
AspS9I GGNCC 1 cut(s) 977
AsuHPI GGTGA 2 cut(s) 439, 611
AvaII GGWCC 1 cut(s) 977
AxyI CCTNAGG 1 cut(s) 1009
BaeGI GKGCMC 1 cut(s) 1061
BalI TGGCCA 1 cut(s) 716
BanI GGYRCC 3 cut(s) 308, 454, 1047
BarI GAAGNNNNNNTAC 4 cut(s) 169, 201, 385, 417
BbsI GAAGAC 1 cut(s) 923
Bbv12I GWGCWC 1 cut(s) 1061
BbvI GCAGC 3 cut(s) 482, 633, 1082
BccI CCATC 2 cut(s) 484, 1083
BceAI ACGGC 1 cut(s) 648
BciT130I CCWGG 2 cut(s) 303, 459
BclI TGATCA 2 cut(s) 385, 444
BcoDI GTCTC 2 cut(s) 223, 725
BcuI ACTAGT 1 cut(s) 161
BfaI CTAG 6 cut(s) 30, 78, 83, 162, 168, 1067
BfmI CTRYAG 1 cut(s) 1100
BfrI CTTAAG 1 cut(s) 101
BfuAI ACCTGC 2 cut(s) 71, 1046
BisI GCNGC 3 cut(s) 471, 622, 1071
BlsI GCNGC 3 cut(s) 472, 623, 1072
Bme1390I CCNGG 2 cut(s) 303, 459
Bme18I GGWCC 1 cut(s) 977
BmgT120I GGNCC 1 cut(s) 977
BmiI GGNNCC 4 cut(s) 310, 456, 463, 1049
BmrFI CCNGG 2 cut(s) 303, 459
BmrI ACTGGG 1 cut(s) 905
BmsI GCATC 3 cut(s) 608, 673, 691
BmuI ACTGGG 1 cut(s) 905
BpiI GAAGAC 1 cut(s) 923
BpmI CTGGAG 2 cut(s) 262, 555
Bpu10I CCTNAGC 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 833
BsaBI GATNNNNATC 1 cut(s) 11
BsaI GGTCTC 1 cut(s) 223
BsaJI CCNNGG 2 cut(s) 180, 450
Bsc4I CCNNNNNNNGG 1 cut(s) 1044
Bse118I RCCGGY 2 cut(s) 87, 235
Bse1I ACTGG 5 cut(s) 47, 279, 572, 717, 911
Bse21I CCTNAGG 1 cut(s) 1009
Bse3DI GCAATG 2 cut(s) 262, 465
Bse8I GATNNNNATC 1 cut(s) 11
BseBI CCWGG 2 cut(s) 303, 459
BseDI CCNNGG 2 cut(s) 180, 450
BseGI GGATG 2 cut(s) 688, 1075
BseJI GATNNNNATC 1 cut(s) 11
BseLI CCNNNNNNNGG 1 cut(s) 1044
BseMI GCAATG 2 cut(s) 262, 465
BseMII CTCAG 2 cut(s) 276, 1023
BseNI ACTGG 5 cut(s) 47, 279, 572, 717, 911
BseRI GAGGAG 2 cut(s) 493, 798
BseSI GKGCMC 1 cut(s) 1061
BseXI GCAGC 3 cut(s) 482, 633, 1082
BseYI CCCAGC 1 cut(s) 473
BshFI GGCC 2 cut(s) 716, 1014
BshNI GGYRCC 3 cut(s) 308, 454, 1047
BsiHKAI GWGCWC 1 cut(s) 1061
BsiSI CCGG 2 cut(s) 88, 236
BslI CCNNNNNNNGG 1 cut(s) 1044
BsmAI GTCTC 2 cut(s) 223, 725
BsnI GGCC 2 cut(s) 716, 1014
Bso31I GGTCTC 1 cut(s) 223
Bsp1286I GDGCHC 1 cut(s) 1061
Bsp143I GATC 5 cut(s) 248, 385, 444, 539, 607
BspANI GGCC 2 cut(s) 716, 1014
BspCNI CTCAG 2 cut(s) 277, 1022
BspHI TCATGA 1 cut(s) 382
BspLI GGNNCC 4 cut(s) 310, 456, 463, 1049
BspMI ACCTGC 2 cut(s) 71, 1046
BspQI GCTCTTC 1 cut(s) 501
BspT107I GGYRCC 3 cut(s) 308, 454, 1047
BspTI CTTAAG 1 cut(s) 101
BspTNI GGTCTC 1 cut(s) 223
BsrDI GCAATG 2 cut(s) 262, 465
BsrFI RCCGGY 2 cut(s) 87, 235
BsrI ACTGG 5 cut(s) 47, 279, 572, 717, 911
BssAI RCCGGY 2 cut(s) 87, 235
BssECI CCNNGG 2 cut(s) 180, 450
BssMI GATC 5 cut(s) 248, 385, 444, 539, 607
BssT1I CCWWGG 1 cut(s) 180
Bst2UI CCWGG 2 cut(s) 303, 459
Bst4CI ACNGT 2 cut(s) 111, 985
Bst6I CTCTTC 3 cut(s) 406, 442, 501
BstAFI CTTAAG 1 cut(s) 101
BstC8I GCNNGC 1 cut(s) 1005
BstDEI CTNAG 3 cut(s) 285, 321, 1009
BstENI CCTNNNNNAGG 1 cut(s) 1042
BstF5I GGATG 2 cut(s) 688, 1075
BstHHI GCGC 1 cut(s) 93
BstKTI GATC 5 cut(s) 251, 388, 447, 542, 610
BstMAI GTCTC 2 cut(s) 223, 725
BstMBI GATC 5 cut(s) 248, 385, 444, 539, 607
BstMWI GCNNNNNNNGC 1 cut(s) 377
BstNI CCWGG 2 cut(s) 303, 459
BstNSI RCATGY 1 cut(s) 506
BstSCI CCNGG 2 cut(s) 301, 457
BstSFI CTRYAG 1 cut(s) 1100
BstSLI GKGCMC 1 cut(s) 1061
BstV1I GCAGC 3 cut(s) 482, 633, 1082
BstV2I GAAGAC 1 cut(s) 923
Bsu36I CCTNAGG 1 cut(s) 1009
BsuRI GGCC 2 cut(s) 716, 1014
BtsCI GGATG 2 cut(s) 688, 1075
BtsIMutI CAGTG 4 cut(s) 54, 723, 918, 1061
BveI ACCTGC 2 cut(s) 71, 1046
Cac8I GCNNGC 1 cut(s) 1005
CciI TCATGA 1 cut(s) 382
CfoI GCGC 1 cut(s) 93
Cfr10I RCCGGY 2 cut(s) 87, 235
Cfr13I GGNCC 1 cut(s) 977
Csp6I GTAC 2 cut(s) 177, 309
CviAII CATG 5 cut(s) 383, 389, 503, 949, 1000
CviQI GTAC 2 cut(s) 177, 309
DdeI CTNAG 3 cut(s) 285, 321, 1009
DpnI GATC 5 cut(s) 250, 387, 446, 541, 609
DpnII GATC 5 cut(s) 248, 385, 444, 539, 607
DraI TTTAAA 1 cut(s) 660
EaeI YGGCCR 1 cut(s) 714
Eam1104I CTCTTC 3 cut(s) 406, 442, 501
EarI CTCTTC 3 cut(s) 406, 442, 501
Eco130I CCWWGG 1 cut(s) 180
Eco147I AGGCCT 1 cut(s) 1014
Eco31I GGTCTC 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 977
Eco57I CTGAAG 3 cut(s) 38, 726, 1037
Eco81I CCTNAGG 1 cut(s) 1009
EcoNI CCTNNNNNAGG 1 cut(s) 1042
EcoRI GAATTC 1 cut(s) 807
EcoRII CCWGG 2 cut(s) 301, 457
EcoT14I CCWWGG 1 cut(s) 180
EcoT22I ATGCAT 1 cut(s) 688
ErhI CCWWGG 1 cut(s) 180
FaeI CATG 5 cut(s) 386, 392, 506, 952, 1003
FatI CATG 5 cut(s) 382, 388, 502, 948, 999
FauNDI CATATG 1 cut(s) 851
FbaI TGATCA 2 cut(s) 385, 444
FblI GTMKAC 2 cut(s) 394, 792
Fnu4HI GCNGC 3 cut(s) 471, 622, 1071
FokI GGATG 2 cut(s) 695, 1062
Fsp4HI GCNGC 3 cut(s) 471, 622, 1071
FspBI CTAG 6 cut(s) 30, 78, 83, 162, 168, 1067
GlaI GCGC 1 cut(s) 92
GluI GCNGC 3 cut(s) 471, 622, 1071
GsaI CCCAGC 1 cut(s) 477
GsuI CTGGAG 2 cut(s) 262, 555
HaeIII GGCC 2 cut(s) 716, 1014
HapII CCGG 2 cut(s) 88, 236
HhaI GCGC 1 cut(s) 93
Hin1II CATG 5 cut(s) 386, 392, 506, 952, 1003
Hin6I GCGC 1 cut(s) 91
HinP1I GCGC 1 cut(s) 91
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HindIII AAGCTT 1 cut(s) 356
HinfI GANTC 3 cut(s) 33, 396, 865
HpaII CCGG 2 cut(s) 88, 236
HphI GGTGA 2 cut(s) 439, 611
Hpy166II GTNNAC 4 cut(s) 114, 395, 793, 1059
Hpy188I TCNGA 6 cut(s) 228, 286, 444, 643, 754, 931
Hpy188III TCNNGA 3 cut(s) 30, 383, 812
Hpy8I GTNNAC 4 cut(s) 114, 395, 793, 1059
HpyAV CCTTC 3 cut(s) 817, 1006, 1061
HpyCH4III ACNGT 2 cut(s) 111, 985
HpyCH4V TGCA 8 cut(s) 66, 470, 506, 551, 621, 686, 1003, 1059
HpyF10VI GCNNNNNNNGC 1 cut(s) 377
HpyF3I CTNAG 3 cut(s) 285, 321, 1009
Hsp92II CATG 5 cut(s) 386, 392, 506, 952, 1003
HspAI GCGC 1 cut(s) 91
KpnI GGTACC 1 cut(s) 312
Ksp22I TGATCA 2 cut(s) 385, 444
Kzo9I GATC 5 cut(s) 248, 385, 444, 539, 607
LguI GCTCTTC 1 cut(s) 501
LmnI GCTCC 2 cut(s) 484, 574
Lsp1109I GCAGC 3 cut(s) 482, 633, 1082
LweI GCATC 3 cut(s) 608, 673, 691
MaeI CTAG 6 cut(s) 30, 78, 83, 162, 168, 1067
MaeIII GTNAC 1 cut(s) 295
MalI GATC 5 cut(s) 250, 387, 446, 541, 609
MboI GATC 5 cut(s) 248, 385, 444, 539, 607
MhlI GDGCHC 1 cut(s) 1061
MlsI TGGCCA 1 cut(s) 716
MluCI AATT 8 cut(s) 94, 171, 204, 553, 637, 807, 827, 876
MluNI TGGCCA 1 cut(s) 716
MlyI GAGTC 1 cut(s) 390
MmeI TCCRAC 1 cut(s) 1004
Mox20I TGGCCA 1 cut(s) 716
Mph1103I ATGCAT 1 cut(s) 688
MscI TGGCCA 1 cut(s) 716
MseI TTAA 3 cut(s) 102, 354, 659
MslI CAYNNNNRTG 1 cut(s) 387
Msp20I TGGCCA 1 cut(s) 716
MspA1I CMGCKG 1 cut(s) 473
MspCI CTTAAG 1 cut(s) 101
MspI CCGG 2 cut(s) 88, 236
MspR9I CCNGG 2 cut(s) 303, 459
MvaI CCWGG 2 cut(s) 303, 459
MwoI GCNNNNNNNGC 1 cut(s) 377
NdeI CATATG 1 cut(s) 851
NdeII GATC 5 cut(s) 248, 385, 444, 539, 607
NlaIII CATG 5 cut(s) 386, 392, 506, 952, 1003
NlaIV GGNNCC 4 cut(s) 310, 456, 463, 1049
NmuCI GTSAC 1 cut(s) 295
NsiI ATGCAT 1 cut(s) 688
NspI RCATGY 1 cut(s) 506
PagI TCATGA 1 cut(s) 382
PaqCI CACCTGC 1 cut(s) 1046
PceI AGGCCT 1 cut(s) 1014
PciSI GCTCTTC 1 cut(s) 501
PfeI GAWTC 2 cut(s) 33, 865
PkrI GCNGC 3 cut(s) 472, 623, 1072
PleI GAGTC 1 cut(s) 390
PpsI GAGTC 1 cut(s) 390
Psp6I CCWGG 2 cut(s) 301, 457
PspFI CCCAGC 1 cut(s) 473
PspGI CCWGG 2 cut(s) 301, 457
PspN4I GGNNCC 4 cut(s) 310, 456, 463, 1049
PspPI GGNCC 1 cut(s) 977
PvuII CAGCTG 1 cut(s) 473
RsaI GTAC 2 cut(s) 178, 310
RsaNI GTAC 2 cut(s) 177, 309
RseI CAYNNNNRTG 1 cut(s) 387
SapI GCTCTTC 1 cut(s) 501
SaqAI TTAA 3 cut(s) 102, 354, 659
SatI GCNGC 3 cut(s) 471, 622, 1071
Sau3AI GATC 5 cut(s) 248, 385, 444, 539, 607
Sau96I GGNCC 1 cut(s) 977
SchI GAGTC 1 cut(s) 390
ScrFI CCNGG 2 cut(s) 303, 459
SduI GDGCHC 1 cut(s) 1061
SfaNI GCATC 3 cut(s) 608, 673, 691
SfcI CTRYAG 1 cut(s) 1100
SgrAI CRCCGGYG 1 cut(s) 235
SinI GGWCC 1 cut(s) 977
SmiMI CAYNNNNRTG 1 cut(s) 387
SmlI CTYRAG 2 cut(s) 101, 812
SmoI CTYRAG 2 cut(s) 101, 812
SpeI ACTAGT 1 cut(s) 161
Sse9I AATT 8 cut(s) 94, 171, 204, 553, 637, 807, 827, 876
SseBI AGGCCT 1 cut(s) 1014
SspMI CTAG 6 cut(s) 30, 78, 83, 162, 168, 1067
StuI AGGCCT 1 cut(s) 1014
StyD4I CCNGG 2 cut(s) 301, 457
StyI CCWWGG 1 cut(s) 180
TaaI ACNGT 2 cut(s) 111, 985
TaqI TCGA 3 cut(s) 70, 410, 743
TasI AATT 8 cut(s) 94, 171, 204, 553, 637, 807, 827, 876
TfiI GAWTC 2 cut(s) 33, 865
Tru1I TTAA 3 cut(s) 102, 354, 659
Tru9I TTAA 3 cut(s) 102, 354, 659
TscAI CASTG 4 cut(s) 54, 730, 918, 1061
TseFI GTSAC 1 cut(s) 295
TseI GCWGC 3 cut(s) 470, 621, 1070
Tsp45I GTSAC 1 cut(s) 295
TspDTI ATGAA 5 cut(s) 177, 533, 616, 980, 988
TspRI CASTG 4 cut(s) 54, 730, 918, 1061
Vha464I CTTAAG 1 cut(s) 101
VneI GTGCAC 1 cut(s) 1057
VpaK11BI GGWCC 1 cut(s) 977
XagI CCTNNNNNAGG 1 cut(s) 1042
XapI RAATTY 3 cut(s) 553, 807, 827
XbaI TCTAGA 1 cut(s) 29
XceI RCATGY 1 cut(s) 506
XmiI GTMKAC 2 cut(s) 394, 792
XspI CTAG 6 cut(s) 30, 78, 83, 162, 168, 1067
Zsp2I ATGCAT 1 cut(s) 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.