Prupe.4G125700_v2.0.a1

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0012861
Physical Location & Seq
Reverse (-)
6893113 .. 6896164
3052 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G125700.1

Sequence Viewer

Length: 1281 bp
ATGTGCAGCTCTAAGGCCAAGGTGACCATAGGGGTTGAAGTCACACCCATGGTTGCTAGAATCAATGGCAGACCTGTCCTTCAGCCTACCTGCAATCGGGTTCCTAGCCTCGACAGGCGGAATTCGATTAAGAAAATATCAACACCACGTGCACCTCCCCCTCCACCATTGCCAACTTCTTCTGCTAGTAGTACTAGTCCTAGAATTTCCAACAAGGCTTCATCATTGTTAACACCTCCAATTTCTCCGAAGTCAAAGTCTCCAAGGCCACCAGCAATAAAGAGAGGAAATGATCCTAATGGGTTGAACTCAAGTTCTGAGAAAGTTGTGACCCCAGGAGGTACTACAAGAGCTAAGATTTTGGAAAGGAAGAAGTCCAAGAGCTTTAAGCGTGCTAGTGTAGGTGTTGATGGTGCTTCTGCTGATCTTCATCATCATGGAGATTTTAGTGCTGGTGGATTTTCTTCTTCTTTGAATATCGAGGCATCATTGAGTTATTCGTCTTCTTTGATCACTGAGGCGCCGGGAAGCATTGCTGCTGTGAGGAGGGAGCAGATGGCCCTTCAACATGCACAAAGGAAGATGAGGATTGCCCATTATGGAAGATCAAAGTCAGCCAACTTTGAAAGGGTTGTTCCTGTTGATGCTTCTGGTAATATAGAAGCCAAGGGAGCTGAAGAAGAGAAAAGGTGCAGCTTTATCACAGCTAATTCAGATCCCATCTATGTTGCTTACCATGATGAAGAATGGGGAGTTCCAGTTCATGATGACAAGATGTTGTTTGAACTGCTTGTTCTGAGTGGAGCTCAAGTTGGCTCAGATTGGACTTCCATTTTAAAGAAACGCCAAGATTTCAGGAATGCATTTTCAGATTTTGATGCAGAAATTGTGGCCAATTTCACTGACAAGCAGATGGTGTCAATTGGTTCAGAATATGGCATTGATATAAGCAGGGTCCGAGGAGTTGTGGACAACTCTAACCGAATTCTTGAGATTAAGAAGGAATTTGGATCATTTGACAAGTACATCTGGGGGTTTGTCAATCAGAAACCCATCTCACCTCAGTACAAATTAGGCTACAAAATCCCAGTTAAGACATCAAAATCAGAGTCCATCAGCAAGGACATGGTTAGGAGGGGATTTAGGTTTGTGGGTCCAACAGTTGTTCATTCTTTCATGCAAGCCTCAGGCCTCACAAACGACCATTTGATCACTTGCCACAGGCACCTCCAGTGTACCTTGTTGGCAGCTCGCCGTCCCACATTGGAAGAAGTTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

427

Amino Acids

46.69

Weight (kDa)

9.77

Isoelectric Point (pI)

47.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012861)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12710
fragaria_vesca FvH4_3g13890
malus_domestica MD05G1240400.v1.1 MD10G1218500.v1.1
prunus_persica Prupe.4G125700_v2.0.a1
pyrus_communis pycom05g21640 pycom10g18430
rosa_chinensis RchiOBHm_Chr5g0023071
rosa_laevigata RLG00000032698
rosa_multiflora Rmu_co8421393.1_g000001
rosa_roxburghii Rroxscaffold_1G00056250
rosa_rugosa Rorug05G0077100
rosa_samantha Rh5AG163200 Rh5BG162500 Rh5CG177300 Rh5DG163700
rosa_wichuraiana Rw5G014720 Rw5G014990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 98
AccB1I GGYRCC 2 cut(s) 520, 1224
AciI CCGC 1 cut(s) 118
AclWI GGATC 3 cut(s) 287, 710, 1018
AcoI YGGCCR 1 cut(s) 891
AcsI RAATTY 4 cut(s) 121, 204, 984, 1004
AcuI CTGAAG 2 cut(s) 65, 696
AcvI CACGTG 1 cut(s) 149
AcyI GRCGYC 1 cut(s) 521
AdeI CACNNNGTG 1 cut(s) 149
AfaI GTAC 5 cut(s) 193, 343, 1025, 1067, 1237
AfiI CCNNNNNNNGG 1 cut(s) 96
AgsI TTSAA 6 cut(s) 38, 307, 475, 566, 626, 785
AhlI ACTAGT 1 cut(s) 194
AjnI CCWGG 1 cut(s) 334
AloI GAACNNNNNNTCC 2 cut(s) 744, 776
AluBI AGCT 8 cut(s) 9, 353, 384, 674, 696, 707, 806, 1250
AluI AGCT 8 cut(s) 9, 353, 384, 674, 696, 707, 806, 1250
Alw21I GWGCWC 2 cut(s) 154, 808
Alw26I GTCTC 1 cut(s) 264
Alw44I GTGCAC 1 cut(s) 150
AlwI GGATC 3 cut(s) 287, 710, 1018
AoxI GGCC 5 cut(s) 15, 266, 558, 891, 1189
ApaLI GTGCAC 1 cut(s) 150
ApeKI GCWGC 4 cut(s) 6, 536, 693, 1247
ApoI RAATTY 4 cut(s) 121, 204, 984, 1004
ArsI GACNNNNNNTTYG 4 cut(s) 242, 274, 1094, 1126
Asp700I GAANNNNTTC 1 cut(s) 1272
AspLEI GCGC 1 cut(s) 523
AspS9I GGNCC 3 cut(s) 559, 955, 1154
AsuC2I CCSGG 1 cut(s) 525
AsuHPI GGTGA 2 cut(s) 34, 1050
AvaII GGWCC 2 cut(s) 955, 1154
AxyI CCTNAGG 1 cut(s) 1186
BaeGI GKGCMC 1 cut(s) 154
BalI TGGCCA 1 cut(s) 893
BanI GGYRCC 2 cut(s) 520, 1224
BanII GRGCYC 1 cut(s) 808
BbrPI CACGTG 1 cut(s) 149
BbsI GAAGAC 1 cut(s) 495
Bbv12I GWGCWC 2 cut(s) 154, 808
BbvI GCAGC 4 cut(s) 18, 523, 705, 1259
BccI CCATC 6 cut(s) 404, 550, 728, 907, 1061, 1121
BceAI ACGGC 1 cut(s) 1239
BciT130I CCWGG 1 cut(s) 336
BclI TGATCA 2 cut(s) 510, 1209
BcnI CCSGG 1 cut(s) 525
BcoDI GTCTC 1 cut(s) 264
BcuI ACTAGT 1 cut(s) 194
BfaI CTAG 6 cut(s) 57, 105, 186, 195, 201, 396
BfmI CTRYAG 1 cut(s) 1277
BfoI RGCGCY 1 cut(s) 524
BfuAI ACCTGC 1 cut(s) 98
BisI GCNGC 4 cut(s) 7, 537, 694, 1248
BlsI GCNGC 4 cut(s) 8, 538, 695, 1249
BmcAI AGTACT 1 cut(s) 193
Bme1390I CCNGG 2 cut(s) 336, 525
Bme18I GGWCC 2 cut(s) 955, 1154
BmgT120I GGNCC 3 cut(s) 559, 955, 1154
BmiI GGNNCC 5 cut(s) 102, 522, 956, 1155, 1226
BmrFI CCNGG 2 cut(s) 336, 525
BmrI ACTGGG 1 cut(s) 1082
BmsI GCATC 3 cut(s) 494, 634, 868
BmuI ACTGGG 1 cut(s) 1082
BpiI GAAGAC 1 cut(s) 495
BplI GAGNNNNNCTC 2 cut(s) 790, 822
BpmI CTGGAG 1 cut(s) 1214
BpuEI CTTGAG 3 cut(s) 295, 792, 1010
BpuMI CCSGG 1 cut(s) 525
BsaAI YACGTR 1 cut(s) 149
BsaBI GATNNNNATC 1 cut(s) 429
BsaHI GRCGYC 1 cut(s) 521
BsaJI CCNNGG 6 cut(s) 18, 48, 263, 334, 666, 958
BsaXI ACNNNNNCTCC 4 cut(s) 744, 774, 795, 825
Bsc4I CCNNNNNNNGG 1 cut(s) 96
Bse1I ACTGG 3 cut(s) 758, 1088, 1231
Bse21I CCTNAGG 1 cut(s) 1186
Bse3DI GCAATG 2 cut(s) 167, 531
Bse8I GATNNNNATC 1 cut(s) 429
BseBI CCWGG 1 cut(s) 336
BseDI CCNNGG 6 cut(s) 18, 48, 263, 334, 666, 958
BseJI GATNNNNATC 1 cut(s) 429
BseLI CCNNNNNNNGG 1 cut(s) 96
BseMI GCAATG 2 cut(s) 167, 531
BseMII CTCAG 6 cut(s) 309, 507, 788, 831, 1076, 1200
BseNI ACTGG 3 cut(s) 758, 1088, 1231
BseRI GAGGAG 2 cut(s) 559, 975
BseSI GKGCMC 1 cut(s) 154
BseXI GCAGC 4 cut(s) 18, 523, 705, 1259
BsgI GTGCAG 2 cut(s) 25, 712
BshFI GGCC 5 cut(s) 17, 268, 560, 893, 1191
BshNI GGYRCC 2 cut(s) 520, 1224
BsiHKAI GWGCWC 2 cut(s) 154, 808
BsiSI CCGG 1 cut(s) 524
BslFI GGGAC 1 cut(s) 1242
BslI CCNNNNNNNGG 1 cut(s) 96
BsmAI GTCTC 1 cut(s) 264
BsmFI GGGAC 1 cut(s) 1242
BsmI GAATGC 1 cut(s) 865
BsnI GGCC 5 cut(s) 17, 268, 560, 893, 1191
Bsp1286I GDGCHC 2 cut(s) 154, 808
Bsp143I GATC 7 cut(s) 292, 424, 510, 605, 715, 1010, 1209
Bsp19I CCATGG 1 cut(s) 48
BspACI CCGC 1 cut(s) 118
BspANI GGCC 5 cut(s) 17, 268, 560, 893, 1191
BspCNI CTCAG 6 cut(s) 310, 508, 789, 830, 1075, 1199
BspHI TCATGA 1 cut(s) 763
BspLI GGNNCC 5 cut(s) 102, 522, 956, 1155, 1226
BspMI ACCTGC 1 cut(s) 98
BspPI GGATC 3 cut(s) 287, 710, 1018
BspT107I GGYRCC 2 cut(s) 520, 1224
BsrDI GCAATG 2 cut(s) 167, 531
BsrI ACTGG 3 cut(s) 758, 1088, 1231
BssECI CCNNGG 6 cut(s) 18, 48, 263, 334, 666, 958
BssMI GATC 7 cut(s) 292, 424, 510, 605, 715, 1010, 1209
BssNI GRCGYC 1 cut(s) 521
BssT1I CCWWGG 4 cut(s) 18, 48, 263, 666
Bst2UI CCWGG 1 cut(s) 336
Bst4CI ACNGT 1 cut(s) 1162
Bst6I CTCTTC 1 cut(s) 675
BstACI GRCGYC 1 cut(s) 521
BstBAI YACGTR 1 cut(s) 149
BstC8I GCNNGC 3 cut(s) 393, 1182, 1252
BstDEI CTNAG 8 cut(s) 12, 318, 354, 516, 797, 817, 1062, 1186
BstDSI CCRYGG 1 cut(s) 48
BstEII GGTNACC 1 cut(s) 22
BstH2I RGCGCY 1 cut(s) 524
BstHHI GCGC 1 cut(s) 523
BstKTI GATC 7 cut(s) 295, 427, 513, 608, 718, 1013, 1212
BstMAI GTCTC 1 cut(s) 264
BstMBI GATC 7 cut(s) 292, 424, 510, 605, 715, 1010, 1209
BstMWI GCNNNNNNNGC 1 cut(s) 671
BstNI CCWGG 1 cut(s) 336
BstNSI RCATGY 1 cut(s) 572
BstPI GGTNACC 1 cut(s) 22
BstSCI CCNGG 2 cut(s) 334, 523
BstSFI CTRYAG 1 cut(s) 1277
BstSLI GKGCMC 1 cut(s) 154
BstV1I GCAGC 4 cut(s) 18, 523, 705, 1259
BstV2I GAAGAC 1 cut(s) 495
BstX2I RGATCY 1 cut(s) 715
BstYI RGATCY 1 cut(s) 715
Bsu36I CCTNAGG 1 cut(s) 1186
BsuRI GGCC 5 cut(s) 17, 268, 560, 893, 1191
BtgI CCRYGG 1 cut(s) 48
BtsIMutI CAGTG 3 cut(s) 513, 900, 1238
BveI ACCTGC 1 cut(s) 98
Cac8I GCNNGC 3 cut(s) 393, 1182, 1252
CciI TCATGA 1 cut(s) 763
CfoI GCGC 1 cut(s) 523
Cfr13I GGNCC 3 cut(s) 559, 955, 1154
Csp6I GTAC 5 cut(s) 192, 342, 1024, 1066, 1236
CviAII CATG 7 cut(s) 49, 437, 569, 737, 764, 1126, 1177
CviQI GTAC 5 cut(s) 192, 342, 1024, 1066, 1236
DdeI CTNAG 8 cut(s) 12, 318, 354, 516, 797, 817, 1062, 1186
DinI GGCGCC 1 cut(s) 522
DpnI GATC 7 cut(s) 294, 426, 512, 607, 717, 1012, 1211
DpnII GATC 7 cut(s) 292, 424, 510, 605, 715, 1010, 1209
DraI TTTAAA 1 cut(s) 837
DraIII CACNNNGTG 1 cut(s) 149
EaeI YGGCCR 1 cut(s) 891
Eam1104I CTCTTC 1 cut(s) 675
EarI CTCTTC 1 cut(s) 675
EciI GGCGGA 1 cut(s) 133
Ecl136II GAGCTC 1 cut(s) 806
Eco130I CCWWGG 4 cut(s) 18, 48, 263, 666
Eco147I AGGCCT 1 cut(s) 1191
Eco24I GRGCYC 1 cut(s) 808
Eco47I GGWCC 2 cut(s) 955, 1154
Eco53kI GAGCTC 1 cut(s) 806
Eco57I CTGAAG 2 cut(s) 65, 696
Eco72I CACGTG 1 cut(s) 149
Eco81I CCTNAGG 1 cut(s) 1186
Eco91I GGTNACC 1 cut(s) 22
EcoICRI GAGCTC 1 cut(s) 806
EcoO65I GGTNACC 1 cut(s) 22
EcoRI GAATTC 2 cut(s) 121, 984
EcoRII CCWGG 1 cut(s) 334
EcoT14I CCWWGG 4 cut(s) 18, 48, 263, 666
EcoT22I ATGCAT 1 cut(s) 865
EcoT38I GRGCYC 1 cut(s) 808
EgeI GGCGCC 1 cut(s) 522
EheI GGCGCC 1 cut(s) 522
ErhI CCWWGG 4 cut(s) 18, 48, 263, 666
FaeI CATG 7 cut(s) 52, 440, 572, 740, 767, 1129, 1180
FaqI GGGAC 1 cut(s) 1242
FatI CATG 7 cut(s) 48, 436, 568, 736, 763, 1125, 1176
FbaI TGATCA 2 cut(s) 510, 1209
Fnu4HI GCNGC 4 cut(s) 7, 537, 694, 1248
FriOI GRGCYC 1 cut(s) 808
Fsp4HI GCNGC 4 cut(s) 7, 537, 694, 1248
FspBI CTAG 6 cut(s) 57, 105, 186, 195, 201, 396
GlaI GCGC 1 cut(s) 522
GluI GCNGC 4 cut(s) 7, 537, 694, 1248
GsuI CTGGAG 1 cut(s) 1214
HaeII RGCGCY 1 cut(s) 524
HaeIII GGCC 5 cut(s) 17, 268, 560, 893, 1191
HapII CCGG 1 cut(s) 524
HhaI GCGC 1 cut(s) 523
Hin1I GRCGYC 1 cut(s) 521
Hin1II CATG 7 cut(s) 52, 440, 572, 740, 767, 1129, 1180
Hin6I GCGC 1 cut(s) 521
HinP1I GCGC 1 cut(s) 521
HincII GTYRAC 1 cut(s) 231
HindII GTYRAC 1 cut(s) 231
HinfI GANTC 2 cut(s) 60, 1109
HpaI GTTAAC 1 cut(s) 231
HpaII CCGG 1 cut(s) 524
HphI GGTGA 2 cut(s) 34, 1050
Hpy166II GTNNAC 4 cut(s) 152, 231, 970, 1236
Hpy188III TCNNGA 3 cut(s) 764, 856, 989
Hpy8I GTNNAC 4 cut(s) 152, 231, 970, 1236
HpyAV CCTTC 3 cut(s) 89, 572, 994
HpyCH4III ACNGT 1 cut(s) 1162
HpyCH4IV ACGT 1 cut(s) 148
HpyCH4V TGCA 8 cut(s) 6, 93, 152, 572, 693, 863, 881, 1180
HpyF10VI GCNNNNNNNGC 1 cut(s) 671
HpyF3I CTNAG 8 cut(s) 12, 318, 354, 516, 797, 817, 1062, 1186
HpySE526I ACGT 1 cut(s) 148
Hsp92I GRCGYC 1 cut(s) 521
Hsp92II CATG 7 cut(s) 52, 440, 572, 740, 767, 1129, 1180
HspAI GCGC 1 cut(s) 521
KasI GGCGCC 1 cut(s) 520
Ksp22I TGATCA 2 cut(s) 510, 1209
KspAI GTTAAC 1 cut(s) 231
Kzo9I GATC 7 cut(s) 292, 424, 510, 605, 715, 1010, 1209
LmnI GCTCC 3 cut(s) 550, 671, 803
Lsp1109I GCAGC 4 cut(s) 18, 523, 705, 1259
LweI GCATC 3 cut(s) 494, 634, 868
MaeI CTAG 6 cut(s) 57, 105, 186, 195, 201, 396
MaeII ACGT 1 cut(s) 148
MaeIII GTNAC 3 cut(s) 22, 40, 328
MalI GATC 7 cut(s) 294, 426, 512, 607, 717, 1012, 1211
MboI GATC 7 cut(s) 292, 424, 510, 605, 715, 1010, 1209
MfeI CAATTG 1 cut(s) 921
MflI RGATCY 1 cut(s) 715
MhlI GDGCHC 2 cut(s) 154, 808
MlsI TGGCCA 1 cut(s) 893
MluNI TGGCCA 1 cut(s) 893
Mly113I GGCGCC 1 cut(s) 521
MlyI GAGTC 1 cut(s) 1118
MmeI TCCRAC 2 cut(s) 234, 1181
Mox20I TGGCCA 1 cut(s) 893
Mph1103I ATGCAT 1 cut(s) 865
MroXI GAANNNNTTC 1 cut(s) 1272
MscI TGGCCA 1 cut(s) 893
MseI TTAA 6 cut(s) 129, 230, 387, 836, 996, 1092
MslI CAYNNNNRTG 2 cut(s) 47, 435
Msp20I TGGCCA 1 cut(s) 893
MspI CCGG 1 cut(s) 524
MspR9I CCNGG 2 cut(s) 336, 525
MunI CAATTG 1 cut(s) 921
Mva1269I GAATGC 1 cut(s) 865
MvaI CCWGG 1 cut(s) 336
MwoI GCNNNNNNNGC 1 cut(s) 671
NarI GGCGCC 1 cut(s) 521
NciI CCSGG 1 cut(s) 525
NcoI CCATGG 1 cut(s) 48
NdeII GATC 7 cut(s) 292, 424, 510, 605, 715, 1010, 1209
NlaIII CATG 7 cut(s) 52, 440, 572, 740, 767, 1129, 1180
NlaIV GGNNCC 5 cut(s) 102, 522, 956, 1155, 1226
NmuCI GTSAC 3 cut(s) 22, 40, 328
NsiI ATGCAT 1 cut(s) 865
NspI RCATGY 1 cut(s) 572
PagI TCATGA 1 cut(s) 763
PceI AGGCCT 1 cut(s) 1191
PctI GAATGC 1 cut(s) 865
PdmI GAANNNNTTC 1 cut(s) 1272
PfeI GAWTC 1 cut(s) 60
PkrI GCNGC 4 cut(s) 8, 538, 695, 1249
PleI GAGTC 1 cut(s) 1117
PluTI GGCGCC 1 cut(s) 524
PmaCI CACGTG 1 cut(s) 149
PmlI CACGTG 1 cut(s) 149
PpsI GAGTC 1 cut(s) 1117
Ppu21I YACGTR 1 cut(s) 149
Psp124BI GAGCTC 1 cut(s) 808
Psp6I CCWGG 1 cut(s) 334
PspCI CACGTG 1 cut(s) 149
PspEI GGTNACC 1 cut(s) 22
PspGI CCWGG 1 cut(s) 334
PspN4I GGNNCC 5 cut(s) 102, 522, 956, 1155, 1226
PspPI GGNCC 3 cut(s) 559, 955, 1154
PsuI RGATCY 1 cut(s) 715
RsaI GTAC 5 cut(s) 193, 343, 1025, 1067, 1237
RsaNI GTAC 5 cut(s) 192, 342, 1024, 1066, 1236
RseI CAYNNNNRTG 2 cut(s) 47, 435
SacI GAGCTC 1 cut(s) 808
SaqAI TTAA 6 cut(s) 129, 230, 387, 836, 996, 1092
SatI GCNGC 4 cut(s) 7, 537, 694, 1248
Sau3AI GATC 7 cut(s) 292, 424, 510, 605, 715, 1010, 1209
Sau96I GGNCC 3 cut(s) 559, 955, 1154
ScaI AGTACT 1 cut(s) 193
SchI GAGTC 1 cut(s) 1118
ScrFI CCNGG 2 cut(s) 336, 525
SduI GDGCHC 2 cut(s) 154, 808
SfaNI GCATC 3 cut(s) 494, 634, 868
SfcI CTRYAG 1 cut(s) 1277
SfoI GGCGCC 1 cut(s) 522
SinI GGWCC 2 cut(s) 955, 1154
SmiMI CAYNNNNRTG 2 cut(s) 47, 435
SmlI CTYRAG 3 cut(s) 310, 807, 989
SmoI CTYRAG 3 cut(s) 310, 807, 989
SpeI ACTAGT 1 cut(s) 194
SseBI AGGCCT 1 cut(s) 1191
SsiI CCGC 1 cut(s) 118
SspDI GGCGCC 1 cut(s) 520
SspMI CTAG 6 cut(s) 57, 105, 186, 195, 201, 396
SstI GAGCTC 1 cut(s) 808
StuI AGGCCT 1 cut(s) 1191
StyD4I CCNGG 2 cut(s) 334, 523
StyI CCWWGG 4 cut(s) 18, 48, 263, 666
TaaI ACNGT 1 cut(s) 1162
TaiI ACGT 1 cut(s) 151
TaqI TCGA 3 cut(s) 111, 125, 480
TatI WGTACW 3 cut(s) 191, 1023, 1065
TfiI GAWTC 1 cut(s) 60
Tru1I TTAA 6 cut(s) 129, 230, 387, 836, 996, 1092
Tru9I TTAA 6 cut(s) 129, 230, 387, 836, 996, 1092
TscAI CASTG 3 cut(s) 520, 907, 1238
TseFI GTSAC 3 cut(s) 22, 40, 328
TseI GCWGC 4 cut(s) 6, 536, 693, 1247
Tsp45I GTSAC 3 cut(s) 22, 40, 328
TspDTI ATGAA 6 cut(s) 210, 419, 752, 756, 1157, 1165
TspRI CASTG 3 cut(s) 520, 907, 1238
VneI GTGCAC 1 cut(s) 150
VpaK11BI GGWCC 2 cut(s) 955, 1154
XapI RAATTY 4 cut(s) 121, 204, 984, 1004
XceI RCATGY 1 cut(s) 572
XmnI GAANNNNTTC 1 cut(s) 1272
XspI CTAG 6 cut(s) 57, 105, 186, 195, 201, 396
ZrmI AGTACT 1 cut(s) 193
Zsp2I ATGCAT 1 cut(s) 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.