FvH4_3g17730

NADH kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0007259
Physical Location & Seq
Forward (+)
11279420 .. 11282122
2703 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g17730.t1

Sequence Viewer

Length: 954 bp
ATGGCGACGAGGAGATTGTTGGTGCTGCTTAAACCGTTCGACGCTTACCCAGCTTCCCAATCAGAAGGCCTTTCTCGCATCGCCAGCCTCCAGCACTTGGAGAATAGGCGAAATGTGCACAAGGATGCAGTAAACTTTTGTCAGAACATTTTGCGCCACAAGCCTGTTGAGTGGAAAGCTCTACTGCGTAAAGATCTAAGGCAGCCCATCCGTGATGTGGACCTTGTTATTACAGTTGGTGGTGATGGCACTATACTACATGCGAGCCATTATATTGATGACTCAGTTCCAGTTCTAGGAGTGAATTCTGACCCCACCCAACCTGAAGAGGTGGAAAAGCACGGGAATGAGTATGATGCTTCCAGAAGCACAGGCTATCTTTGTGCTGCAACTGTTGACAACTTTGAACAGATACTGGACAACATCATAGAGGATCGAATTAGTCCTTCTGATCTGAGAAGGATCTCCATAGATGTAAACTCGCAAGTGATTTCCACTTATGCTCTTAATGATATTTTAATTGCACATCCATGTCCAGCAACACTTTCACGGTTCTCATTCAAGGTCCTTGTTTGCAGAATCAAAAGTGATGACCAGCCATCCTCTCCTTTAGTAAACTCTCGATCAAGCGGTCTCAGAGTTTCCACCGCTGCTGGAGCAACAGCTGCAATGCTCTCAGCTGGTGGATTTCCAATGCCTATATTATCTCAGGACCTCCAATATATGGTAAGAGAGCCTATTTCGTCAGGAGAGGTCTCAAGCTTAATGCATGGGTTTATCAAACCTCACCAATCCATGGAAGCTACATGGTTTTGTAGAGAGGGTATTTTATACATTGATGGCTCTCATGTCCGCTGTTCCGTAAAAAATGGGGATCGCATTGAAATCTCTTCCAAGGCCCCAGTTTTGAAGGTTTTCTTGCCTCATCGTGTACAGCACGTTAGTAGTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

318

Amino Acids

35.1

Weight (kDa)

6.86

Isoelectric Point (pI)

62.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_kinase PF01513 67 - 102 2.5e-09 ATP-NAD kinase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 97, 724, 796
AciI CCGC 3 cut(s) 630, 648, 853
AclWI GGATC 3 cut(s) 441, 470, 882
AcsI RAATTY 1 cut(s) 304
AcuI CTGAAG 1 cut(s) 345
AfaI GTAC 1 cut(s) 933
AfiI CCNNNNNNNGG 5 cut(s) 97, 217, 296, 724, 796
AgsI TTSAA 4 cut(s) 407, 562, 884, 910
AluBI AGCT 6 cut(s) 53, 179, 665, 680, 762, 803
AluI AGCT 6 cut(s) 53, 179, 665, 680, 762, 803
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 2 cut(s) 638, 760
Alw44I GTGCAC 1 cut(s) 116
AlwI GGATC 3 cut(s) 441, 470, 882
AlwNI CAGNNNCTG 1 cut(s) 415
AoxI GGCC 2 cut(s) 67, 897
ApaLI GTGCAC 1 cut(s) 116
ApeKI GCWGC 5 cut(s) 25, 202, 386, 650, 665
ApoI RAATTY 1 cut(s) 304
Asp700I GAANNNNTTC 1 cut(s) 914
AspLEI GCGC 1 cut(s) 156
AspS9I GGNCC 4 cut(s) 220, 565, 712, 898
AsuHPI GGTGA 2 cut(s) 254, 779
AvaII GGWCC 3 cut(s) 220, 565, 712
BaeGI GKGCMC 1 cut(s) 120
Bbv12I GWGCWC 1 cut(s) 120
BbvI GCAGC 5 cut(s) 12, 214, 373, 637, 652
BccI CCATC 4 cut(s) 215, 239, 607, 833
BcoDI GTCTC 2 cut(s) 638, 760
BfaI CTAG 1 cut(s) 296
BglII AGATCT 1 cut(s) 193
BisI GCNGC 5 cut(s) 26, 203, 387, 651, 666
BlsI GCNGC 5 cut(s) 27, 204, 388, 652, 667
Bme18I GGWCC 3 cut(s) 220, 565, 712
BmgT120I GGNCC 4 cut(s) 220, 565, 712, 898
BmiI GGNNCC 1 cut(s) 900
BmrI ACTGGG 1 cut(s) 896
BmsI GCATC 3 cut(s) 87, 115, 346
BmuI ACTGGG 1 cut(s) 896
BpmI CTGGAG 2 cut(s) 74, 675
BpuEI CTTGAG 1 cut(s) 742
BsaI GGTCTC 2 cut(s) 638, 760
BsaJI CCNNGG 2 cut(s) 795, 894
Bsc4I CCNNNNNNNGG 5 cut(s) 97, 217, 296, 724, 796
Bse1I ACTGG 3 cut(s) 290, 420, 902
Bse3DI GCAATG 1 cut(s) 675
BseDI CCNNGG 2 cut(s) 795, 894
BseGI GGATG 4 cut(s) 130, 207, 526, 599
BseLI CCNNNNNNNGG 5 cut(s) 97, 217, 296, 724, 796
BseMI GCAATG 1 cut(s) 675
BseMII CTCAG 5 cut(s) 297, 446, 649, 690, 722
BseNI ACTGG 3 cut(s) 290, 420, 902
BseRI GAGGAG 1 cut(s) 25
BseSI GKGCMC 1 cut(s) 120
BseXI GCAGC 5 cut(s) 12, 214, 373, 637, 652
BseYI CCCAGC 1 cut(s) 49
BshFI GGCC 2 cut(s) 69, 899
BsiHKAI GWGCWC 1 cut(s) 120
BslI CCNNNNNNNGG 5 cut(s) 97, 217, 296, 724, 796
BsmAI GTCTC 2 cut(s) 638, 760
BsnI GGCC 2 cut(s) 69, 899
Bso31I GGTCTC 2 cut(s) 638, 760
Bsp1286I GDGCHC 1 cut(s) 120
Bsp1407I TGTACA 1 cut(s) 931
Bsp143I GATC 6 cut(s) 193, 433, 451, 462, 623, 874
Bsp19I CCATGG 1 cut(s) 795
BspACI CCGC 3 cut(s) 630, 648, 853
BspANI GGCC 2 cut(s) 69, 899
BspCNI CTCAG 5 cut(s) 296, 447, 648, 689, 721
BspLI GGNNCC 1 cut(s) 900
BspPI GGATC 3 cut(s) 441, 470, 882
BspTNI GGTCTC 2 cut(s) 638, 760
BsrDI GCAATG 1 cut(s) 675
BsrGI TGTACA 1 cut(s) 931
BsrI ACTGG 3 cut(s) 290, 420, 902
BssECI CCNNGG 2 cut(s) 795, 894
BssMI GATC 6 cut(s) 193, 433, 451, 462, 623, 874
BssT1I CCWWGG 2 cut(s) 795, 894
Bst4CI ACNGT 4 cut(s) 36, 235, 394, 552
Bst6I CTCTTC 2 cut(s) 321, 895
BstAPI GCANNNNNTGC 1 cut(s) 665
BstAUI TGTACA 1 cut(s) 931
BstC8I GCNNGC 2 cut(s) 85, 265
BstDEI CTNAG 6 cut(s) 197, 283, 455, 635, 676, 708
BstDSI CCRYGG 1 cut(s) 795
BstF5I GGATG 4 cut(s) 130, 207, 526, 599
BstHHI GCGC 1 cut(s) 156
BstKTI GATC 6 cut(s) 196, 436, 454, 465, 626, 877
BstMAI GTCTC 2 cut(s) 638, 760
BstMBI GATC 6 cut(s) 193, 433, 451, 462, 623, 874
BstMWI GCNNNNNNNGC 7 cut(s) 50, 75, 84, 115, 160, 656, 665
BstNSI RCATGY 1 cut(s) 263
BstSLI GKGCMC 1 cut(s) 120
BstV1I GCAGC 5 cut(s) 12, 214, 373, 637, 652
BstX2I RGATCY 2 cut(s) 193, 462
BstYI RGATCY 2 cut(s) 193, 462
BsuRI GGCC 2 cut(s) 69, 899
BtgI CCRYGG 1 cut(s) 795
BtgZI GCGATG 1 cut(s) 64
BtsCI GGATG 4 cut(s) 130, 207, 526, 599
Cac8I GCNNGC 2 cut(s) 85, 265
CaiI CAGNNNCTG 1 cut(s) 415
CfoI GCGC 1 cut(s) 156
Cfr13I GGNCC 4 cut(s) 220, 565, 712, 898
CseI GACGC 1 cut(s) 50
Csp6I GTAC 1 cut(s) 932
CviAII CATG 6 cut(s) 260, 531, 770, 796, 807, 848
CviQI GTAC 1 cut(s) 932
DdeI CTNAG 6 cut(s) 197, 283, 455, 635, 676, 708
DpnI GATC 6 cut(s) 195, 435, 453, 464, 625, 876
DpnII GATC 6 cut(s) 193, 433, 451, 462, 623, 874
Eam1104I CTCTTC 2 cut(s) 321, 895
EarI CTCTTC 2 cut(s) 321, 895
Eco130I CCWWGG 2 cut(s) 795, 894
Eco147I AGGCCT 1 cut(s) 69
Eco31I GGTCTC 2 cut(s) 638, 760
Eco47I GGWCC 3 cut(s) 220, 565, 712
Eco57I CTGAAG 1 cut(s) 345
EcoO109I RGGNCCY 3 cut(s) 565, 712, 898
EcoRI GAATTC 1 cut(s) 304
EcoT14I CCWWGG 2 cut(s) 795, 894
EcoT22I ATGCAT 1 cut(s) 771
ErhI CCWWGG 2 cut(s) 795, 894
FaeI CATG 6 cut(s) 263, 534, 773, 799, 810, 851
FalI AAGNNNNNCTT 2 cut(s) 902, 934
FatI CATG 6 cut(s) 259, 530, 769, 795, 806, 847
Fnu4HI GCNGC 5 cut(s) 26, 203, 387, 651, 666
FokI GGATG 4 cut(s) 137, 194, 513, 586
Fsp4HI GCNGC 5 cut(s) 26, 203, 387, 651, 666
FspBI CTAG 1 cut(s) 296
GlaI GCGC 1 cut(s) 155
GluI GCNGC 5 cut(s) 26, 203, 387, 651, 666
GsaI CCCAGC 1 cut(s) 53
GsuI CTGGAG 2 cut(s) 74, 675
HaeIII GGCC 2 cut(s) 69, 899
HgaI GACGC 1 cut(s) 50
HhaI GCGC 1 cut(s) 156
Hin1II CATG 6 cut(s) 263, 534, 773, 799, 810, 851
Hin6I GCGC 1 cut(s) 154
HinP1I GCGC 1 cut(s) 154
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HindIII AAGCTT 1 cut(s) 760
HinfI GANTC 2 cut(s) 281, 579
HphI GGTGA 2 cut(s) 254, 779
Hpy166II GTNNAC 7 cut(s) 118, 133, 220, 397, 478, 616, 932
Hpy188I TCNGA 6 cut(s) 64, 144, 310, 451, 456, 638
Hpy188III TCNNGA 4 cut(s) 363, 621, 710, 747
Hpy8I GTNNAC 7 cut(s) 118, 133, 220, 397, 478, 616, 932
Hpy99I CGWCG 2 cut(s) 10, 44
HpyAV CCTTC 4 cut(s) 59, 453, 456, 904
HpyCH4III ACNGT 4 cut(s) 36, 235, 394, 552
HpyCH4IV ACGT 1 cut(s) 939
HpyCH4V TGCA 7 cut(s) 118, 128, 389, 524, 576, 668, 769
HpyF10VI GCNNNNNNNGC 7 cut(s) 50, 75, 84, 115, 160, 656, 665
HpyF3I CTNAG 6 cut(s) 197, 283, 455, 635, 676, 708
HpySE526I ACGT 1 cut(s) 939
Hsp92II CATG 6 cut(s) 263, 534, 773, 799, 810, 851
HspAI GCGC 1 cut(s) 154
Kzo9I GATC 6 cut(s) 193, 433, 451, 462, 623, 874
LmnI GCTCC 1 cut(s) 656
Lsp1109I GCAGC 5 cut(s) 12, 214, 373, 637, 652
LweI GCATC 3 cut(s) 87, 115, 346
MaeI CTAG 1 cut(s) 296
MaeII ACGT 1 cut(s) 939
MalI GATC 6 cut(s) 195, 435, 453, 464, 625, 876
MboI GATC 6 cut(s) 193, 433, 451, 462, 623, 874
MboII GAAGA 2 cut(s) 338, 882
MflI RGATCY 2 cut(s) 193, 462
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 3 cut(s) 304, 438, 519
MlyI GAGTC 1 cut(s) 275
Mph1103I ATGCAT 1 cut(s) 771
MroXI GAANNNNTTC 1 cut(s) 914
MseI TTAA 4 cut(s) 30, 507, 518, 764
MslI CAYNNNNRTG 3 cut(s) 123, 345, 529
MspA1I CMGCKG 4 cut(s) 650, 665, 680, 855
MwoI GCNNNNNNNGC 7 cut(s) 50, 75, 84, 115, 160, 656, 665
NcoI CCATGG 1 cut(s) 795
NdeII GATC 6 cut(s) 193, 433, 451, 462, 623, 874
NlaIII CATG 6 cut(s) 263, 534, 773, 799, 810, 851
NlaIV GGNNCC 1 cut(s) 900
NsiI ATGCAT 1 cut(s) 771
NspI RCATGY 1 cut(s) 263
PceI AGGCCT 1 cut(s) 69
PdmI GAANNNNTTC 1 cut(s) 914
PfeI GAWTC 1 cut(s) 579
PflMI CCANNNNNTGG 3 cut(s) 97, 724, 796
PkrI GCNGC 5 cut(s) 27, 204, 388, 652, 667
PleI GAGTC 1 cut(s) 275
PpsI GAGTC 1 cut(s) 275
PpuMI RGGWCCY 2 cut(s) 565, 712
Psp5II RGGWCCY 2 cut(s) 565, 712
PspFI CCCAGC 1 cut(s) 49
PspN4I GGNNCC 1 cut(s) 900
PspPI GGNCC 4 cut(s) 220, 565, 712, 898
PspPPI RGGWCCY 2 cut(s) 565, 712
PstNI CAGNNNCTG 1 cut(s) 415
PsuI RGATCY 2 cut(s) 193, 462
PvuII CAGCTG 2 cut(s) 665, 680
RsaI GTAC 1 cut(s) 933
RsaNI GTAC 1 cut(s) 932
RseI CAYNNNNRTG 3 cut(s) 123, 345, 529
SaqAI TTAA 4 cut(s) 30, 507, 518, 764
SatI GCNGC 5 cut(s) 26, 203, 387, 651, 666
Sau3AI GATC 6 cut(s) 193, 433, 451, 462, 623, 874
Sau96I GGNCC 4 cut(s) 220, 565, 712, 898
SchI GAGTC 1 cut(s) 275
SduI GDGCHC 1 cut(s) 120
SfaNI GCATC 3 cut(s) 87, 115, 346
SinI GGWCC 3 cut(s) 220, 565, 712
SmiMI CAYNNNNRTG 3 cut(s) 123, 345, 529
SmlI CTYRAG 1 cut(s) 757
SmoI CTYRAG 1 cut(s) 757
Sse9I AATT 3 cut(s) 304, 438, 519
SseBI AGGCCT 1 cut(s) 69
SsiI CCGC 3 cut(s) 630, 648, 853
SspMI CTAG 1 cut(s) 296
StuI AGGCCT 1 cut(s) 69
StyI CCWWGG 2 cut(s) 795, 894
TaaI ACNGT 4 cut(s) 36, 235, 394, 552
TaiI ACGT 1 cut(s) 942
TaqI TCGA 3 cut(s) 39, 436, 622
TasI AATT 3 cut(s) 304, 438, 519
TatI WGTACW 1 cut(s) 931
TfiI GAWTC 1 cut(s) 579
Tru1I TTAA 4 cut(s) 30, 507, 518, 764
Tru9I TTAA 4 cut(s) 30, 507, 518, 764
TseI GCWGC 5 cut(s) 25, 202, 386, 650, 665
TspGWI ACGGA 2 cut(s) 200, 850
Van91I CCANNNNNTGG 3 cut(s) 97, 724, 796
VneI GTGCAC 1 cut(s) 116
VpaK11BI GGWCC 3 cut(s) 220, 565, 712
XapI RAATTY 1 cut(s) 304
XceI RCATGY 1 cut(s) 263
XcmI CCANNNNNNNNNTGG 1 cut(s) 214
XmnI GAANNNNTTC 1 cut(s) 914
XspI CTAG 1 cut(s) 296
Zsp2I ATGCAT 1 cut(s) 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.