Prupe.4G156400_v2.0.a1

NADH kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0007259
Physical Location & Seq
Forward (+)
8960845 .. 8964370
3526 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G156400.1

Sequence Viewer

Length: 1059 bp
ATGCAAAGTCTAGTCTTGGCCGACCTTTTGGCTGAACAACCGTCATACGCTTCACATAGACGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAATGGCGACAAAGAGATTGTTGCTGCTGTTAAAACCGTTCGACGTTTACCCAGTTTCCCAATCAGACGGTCTGGCTCGCATCACCAACCCTCAGGAATTGCAGATATTACATTACTTGGACAACAGGCGAAAGGTGCACAAGGATGCAATAAACTTTTGTCAGAACATTTTGCACCACAAGCCTGTTGAGTGGAAATCCATAGTACGCAACAATCTATCAGAGCGCATCCGTGATGTGGACCTTGTTGTTACAGTTGGTGGTGATGGCACTCTGTTACAAGGTAGCCATTTCATTGATGACTCGGTTCCAGTTCTAGGAGTGAATTCTGACCCCACACAACCTGAAGAGGTTGAGAAGCTCAGTAATGAAATTGATGCTAATAGAAGCACAGGCTATCTTTGCGGTGCAACTGTCAACAACTTTGAACAGGTACTGGACAGTATCCTCGAGGATCGAGCTATTCCTTCCAAATTGACAAGGATATCTGTATCCGTAAACTCACAACCGCTTTCAACTTATGCTCTTAATGATATGTTAATTGCACATCCATGTCCAGCGGCACTTTCACGGTTCTCATTCAAAATTAAAGGGGATGACCAGCCATGCGCCCCCTTTGCGCACTCTCGATCAAGTGGTCTCAGAGTTTCAACAGCTGCTGGATCAACAGCTGCAATGCTCTCAGCAGGCGGGTTTCCGATGCCCATATTATCCCAGGATCTCCAGTATATGGTAAGAGAGCCCATCTCACCAGGAGTGGCCTCAAGCTTAATGCACGGGTTAATCAAATCTCATCAGTCCATGGAAGCTACATGGTTTGGTAAAAAGGGGGTCATATATATTGATGGTTCTCATGTTTCCCATACTATAAAAAATGGGGATGCCATTGAGATATCTTCCAAGGCCCCAATTTTGAAGGTTTTCTTGCCTCACCATTTACAGAACATTAGTGAAGAAACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

353

Amino Acids

39.06

Weight (kDa)

6.54

Isoelectric Point (pI)

55.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 545
Acc16I TGCGCA 1 cut(s) 717
AccB7I CCANNNNNTGG 1 cut(s) 826
AciI CCGC 4 cut(s) 501, 605, 656, 786
AclWI GGATC 3 cut(s) 558, 766, 822
AcoI YGGCCR 1 cut(s) 18
AcsI RAATTY 1 cut(s) 421
AcuI CTGAAG 1 cut(s) 462
AfaI GTAC 2 cut(s) 303, 531
AfiI CCNNNNNNNGG 3 cut(s) 334, 413, 826
AgsI TTSAA 5 cut(s) 524, 612, 679, 747, 1012
AjnI CCWGG 2 cut(s) 810, 847
AluBI AGCT 6 cut(s) 457, 557, 752, 767, 864, 905
AluI AGCT 6 cut(s) 457, 557, 752, 767, 864, 905
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 1 cut(s) 740
Alw44I GTGCAC 1 cut(s) 233
AlwI GGATC 3 cut(s) 558, 766, 822
AlwNI CAGNNNCTG 2 cut(s) 532, 755
Ama87I CYCGRG 1 cut(s) 545
AoxI GGCC 3 cut(s) 18, 855, 999
ApaLI GTGCAC 1 cut(s) 233
ApeKI GCWGC 3 cut(s) 121, 752, 767
ApoI RAATTY 1 cut(s) 421
Asp700I GAANNNNTTC 1 cut(s) 1016
AspLEI GCGC 3 cut(s) 324, 707, 718
AspS9I GGNCC 2 cut(s) 337, 1000
AsuHPI GGTGA 4 cut(s) 172, 371, 837, 1019
AvaI CYCGRG 1 cut(s) 545
AvaII GGWCC 1 cut(s) 337
AxyI CCTNAGG 1 cut(s) 189
BaeGI GKGCMC 1 cut(s) 237
BanII GRGCYC 1 cut(s) 840
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 3 cut(s) 108, 739, 754
BccI CCATC 3 cut(s) 356, 848, 935
BciT130I CCWGG 2 cut(s) 812, 849
BciVI GTATCC 2 cut(s) 551, 598
BcoDI GTCTC 1 cut(s) 740
BfaI CTAG 2 cut(s) 11, 413
BfuI GTATCC 2 cut(s) 551, 598
BisI GCNGC 4 cut(s) 122, 657, 753, 768
BlsI GCNGC 4 cut(s) 123, 658, 754, 769
Bme1390I CCNGG 2 cut(s) 812, 849
Bme18I GGWCC 1 cut(s) 337
BmeT110I CYCGRG 1 cut(s) 545
BmgT120I GGNCC 2 cut(s) 337, 1000
BmiI GGNNCC 2 cut(s) 405, 1002
BmrFI CCNGG 2 cut(s) 812, 849
BmrI ACTGGG 1 cut(s) 143
BmsI GCATC 6 cut(s) 186, 232, 333, 463, 786, 967
BmuI ACTGGG 1 cut(s) 143
BplI GAGNNNNNCTC 2 cut(s) 827, 859
BpmI CTGGAG 1 cut(s) 803
BpuEI CTTGAG 1 cut(s) 844
BsaI GGTCTC 1 cut(s) 740
BsaJI CCNNGG 3 cut(s) 810, 897, 996
Bsc4I CCNNNNNNNGG 3 cut(s) 334, 413, 826
Bse1I ACTGG 4 cut(s) 149, 407, 537, 820
Bse21I CCTNAGG 1 cut(s) 189
Bse3DI GCAATG 1 cut(s) 777
BseBI CCWGG 2 cut(s) 812, 849
BseDI CCNNGG 3 cut(s) 810, 897, 996
BseGI GGATG 5 cut(s) 247, 324, 643, 697, 982
BseLI CCNNNNNNNGG 3 cut(s) 334, 413, 826
BseMI GCAATG 1 cut(s) 777
BseMII CTCAG 4 cut(s) 203, 472, 751, 792
BseNI ACTGG 4 cut(s) 149, 407, 537, 820
BseSI GKGCMC 1 cut(s) 237
BseXI GCAGC 3 cut(s) 108, 739, 754
BshFI GGCC 3 cut(s) 20, 857, 1001
BsiHKAI GWGCWC 1 cut(s) 237
BsiHKCI CYCGRG 1 cut(s) 545
BslI CCNNNNNNNGG 3 cut(s) 334, 413, 826
BsmAI GTCTC 1 cut(s) 740
BsnI GGCC 3 cut(s) 20, 857, 1001
Bso31I GGTCTC 1 cut(s) 740
BsoBI CYCGRG 1 cut(s) 545
Bsp1286I GDGCHC 2 cut(s) 237, 840
Bsp143I GATC 4 cut(s) 550, 725, 758, 814
Bsp19I CCATGG 1 cut(s) 897
BspACI CCGC 4 cut(s) 501, 605, 656, 786
BspANI GGCC 3 cut(s) 20, 857, 1001
BspCNI CTCAG 4 cut(s) 202, 471, 750, 791
BspLI GGNNCC 2 cut(s) 405, 1002
BspPI GGATC 3 cut(s) 558, 766, 822
BspTNI GGTCTC 1 cut(s) 740
BsrDI GCAATG 1 cut(s) 777
BsrI ACTGG 4 cut(s) 149, 407, 537, 820
BssECI CCNNGG 3 cut(s) 810, 897, 996
BssMI GATC 4 cut(s) 550, 725, 758, 814
BssT1I CCWWGG 2 cut(s) 897, 996
Bst2UI CCWGG 2 cut(s) 812, 849
Bst4CI ACNGT 7 cut(s) 42, 135, 167, 352, 511, 539, 669
Bst6I CTCTTC 1 cut(s) 438
BstC8I GCNNGC 2 cut(s) 175, 784
BstDEI CTNAG 4 cut(s) 189, 458, 737, 778
BstDSI CCRYGG 1 cut(s) 897
BstF5I GGATG 5 cut(s) 247, 324, 643, 697, 982
BstHHI GCGC 3 cut(s) 324, 707, 718
BstKTI GATC 4 cut(s) 553, 728, 761, 817
BstMAI GTCTC 1 cut(s) 740
BstMBI GATC 4 cut(s) 550, 725, 758, 814
BstMWI GCNNNNNNNGC 4 cut(s) 232, 277, 498, 713
BstNI CCWGG 2 cut(s) 812, 849
BstSCI CCNGG 2 cut(s) 810, 847
BstSLI GKGCMC 1 cut(s) 237
BstV1I GCAGC 3 cut(s) 108, 739, 754
BstX2I RGATCY 1 cut(s) 814
BstYI RGATCY 1 cut(s) 814
Bsu36I CCTNAGG 1 cut(s) 189
BsuI GTATCC 2 cut(s) 551, 598
BsuRI GGCC 3 cut(s) 20, 857, 1001
BtgI CCRYGG 1 cut(s) 897
BtsCI GGATG 5 cut(s) 247, 324, 643, 697, 982
Cac8I GCNNGC 2 cut(s) 175, 784
CaiI CAGNNNCTG 2 cut(s) 532, 755
CfoI GCGC 3 cut(s) 324, 707, 718
Cfr13I GGNCC 2 cut(s) 337, 1000
Csp6I GTAC 2 cut(s) 302, 530
CviAII CATG 5 cut(s) 648, 702, 898, 909, 950
CviQI GTAC 2 cut(s) 302, 530
DdeI CTNAG 4 cut(s) 189, 458, 737, 778
DpnI GATC 4 cut(s) 552, 727, 760, 816
DpnII GATC 4 cut(s) 550, 725, 758, 814
EaeI YGGCCR 1 cut(s) 18
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
Eco130I CCWWGG 2 cut(s) 897, 996
Eco24I GRGCYC 1 cut(s) 840
Eco31I GGTCTC 1 cut(s) 740
Eco32I GATATC 2 cut(s) 582, 990
Eco47I GGWCC 1 cut(s) 337
Eco57I CTGAAG 1 cut(s) 462
Eco81I CCTNAGG 1 cut(s) 189
Eco88I CYCGRG 1 cut(s) 545
EcoO109I RGGNCCY 1 cut(s) 1000
EcoRI GAATTC 1 cut(s) 421
EcoRII CCWGG 2 cut(s) 810, 847
EcoRV GATATC 2 cut(s) 582, 990
EcoT14I CCWWGG 2 cut(s) 897, 996
EcoT38I GRGCYC 1 cut(s) 840
ErhI CCWWGG 2 cut(s) 897, 996
FaeI CATG 5 cut(s) 651, 705, 901, 912, 953
FalI AAGNNNNNCTT 2 cut(s) 1004, 1036
FatI CATG 5 cut(s) 647, 701, 897, 908, 949
FauI CCCGC 1 cut(s) 779
Fnu4HI GCNGC 4 cut(s) 122, 657, 753, 768
FokI GGATG 5 cut(s) 254, 311, 630, 704, 989
FriOI GRGCYC 1 cut(s) 840
Fsp4HI GCNGC 4 cut(s) 122, 657, 753, 768
FspBI CTAG 2 cut(s) 11, 413
FspI TGCGCA 1 cut(s) 717
GlaI GCGC 3 cut(s) 323, 706, 717
GluI GCNGC 4 cut(s) 122, 657, 753, 768
GsuI CTGGAG 1 cut(s) 803
HaeIII GGCC 3 cut(s) 20, 857, 1001
HhaI GCGC 3 cut(s) 324, 707, 718
Hin1II CATG 5 cut(s) 651, 705, 901, 912, 953
Hin6I GCGC 3 cut(s) 322, 705, 716
HinP1I GCGC 3 cut(s) 322, 705, 716
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HindIII AAGCTT 1 cut(s) 862
HinfI GANTC 1 cut(s) 398
HphI GGTGA 4 cut(s) 172, 371, 837, 1019
Hpy166II GTNNAC 5 cut(s) 145, 235, 337, 514, 595
Hpy188I TCNGA 6 cut(s) 163, 261, 319, 427, 740, 795
Hpy188III TCNNGA 2 cut(s) 191, 723
Hpy8I GTNNAC 5 cut(s) 145, 235, 337, 514, 595
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 2 cut(s) 573, 1006
HpyCH4III ACNGT 7 cut(s) 42, 135, 167, 352, 511, 539, 669
HpyCH4IV ACGT 2 cut(s) 141, 1055
HpyCH4V TGCA 9 cut(s) 4, 199, 235, 245, 271, 506, 641, 770, 871
HpyF10VI GCNNNNNNNGC 4 cut(s) 232, 277, 498, 713
HpyF3I CTNAG 4 cut(s) 189, 458, 737, 778
HpySE526I ACGT 2 cut(s) 141, 1055
Hsp92II CATG 5 cut(s) 651, 705, 901, 912, 953
HspAI GCGC 3 cut(s) 322, 705, 716
Kzo9I GATC 4 cut(s) 550, 725, 758, 814
Lsp1109I GCAGC 3 cut(s) 108, 739, 754
LweI GCATC 6 cut(s) 186, 232, 333, 463, 786, 967
MaeI CTAG 2 cut(s) 11, 413
MaeII ACGT 2 cut(s) 141, 1055
MaeIII GTNAC 2 cut(s) 346, 372
MalI GATC 4 cut(s) 552, 727, 760, 816
MboI GATC 4 cut(s) 550, 725, 758, 814
MboII GAAGA 2 cut(s) 455, 984
MflI RGATCY 1 cut(s) 814
MhlI GDGCHC 2 cut(s) 237, 840
MluCI AATT 7 cut(s) 194, 421, 468, 569, 636, 681, 1005
MlyI GAGTC 1 cut(s) 392
MnlI CCTC 6 cut(s) 198, 439, 541, 554, 868, 1035
MroXI GAANNNNTTC 1 cut(s) 1016
MseI TTAA 6 cut(s) 128, 624, 635, 684, 866, 878
MslI CAYNNNNRTG 2 cut(s) 240, 646
MspA1I CMGCKG 3 cut(s) 656, 752, 767
MspR9I CCNGG 2 cut(s) 812, 849
MvaI CCWGG 2 cut(s) 812, 849
MwoI GCNNNNNNNGC 4 cut(s) 232, 277, 498, 713
NcoI CCATGG 1 cut(s) 897
NdeII GATC 4 cut(s) 550, 725, 758, 814
NlaIII CATG 5 cut(s) 651, 705, 901, 912, 953
NlaIV GGNNCC 2 cut(s) 405, 1002
NsbI TGCGCA 1 cut(s) 717
PaeR7I CTCGAG 1 cut(s) 545
PdmI GAANNNNTTC 1 cut(s) 1016
PflMI CCANNNNNTGG 1 cut(s) 826
PkrI GCNGC 4 cut(s) 123, 658, 754, 769
PleI GAGTC 1 cut(s) 392
PpsI GAGTC 1 cut(s) 392
Psp6I CCWGG 2 cut(s) 810, 847
PspGI CCWGG 2 cut(s) 810, 847
PspN4I GGNNCC 2 cut(s) 405, 1002
PspPI GGNCC 2 cut(s) 337, 1000
PspXI VCTCGAGB 1 cut(s) 545
PstNI CAGNNNCTG 2 cut(s) 532, 755
PsuI RGATCY 1 cut(s) 814
PvuII CAGCTG 2 cut(s) 752, 767
RsaI GTAC 2 cut(s) 303, 531
RsaNI GTAC 2 cut(s) 302, 530
RseI CAYNNNNRTG 2 cut(s) 240, 646
SaqAI TTAA 6 cut(s) 128, 624, 635, 684, 866, 878
SatI GCNGC 4 cut(s) 122, 657, 753, 768
Sau3AI GATC 4 cut(s) 550, 725, 758, 814
Sau96I GGNCC 2 cut(s) 337, 1000
SchI GAGTC 1 cut(s) 392
ScrFI CCNGG 2 cut(s) 812, 849
SduI GDGCHC 2 cut(s) 237, 840
SfaNI GCATC 6 cut(s) 186, 232, 333, 463, 786, 967
Sfr274I CTCGAG 1 cut(s) 545
SinI GGWCC 1 cut(s) 337
SlaI CTCGAG 1 cut(s) 545
SmiMI CAYNNNNRTG 2 cut(s) 240, 646
SmlI CTYRAG 2 cut(s) 545, 859
SmoI CTYRAG 2 cut(s) 545, 859
Sse9I AATT 7 cut(s) 194, 421, 468, 569, 636, 681, 1005
SsiI CCGC 4 cut(s) 501, 605, 656, 786
SspMI CTAG 2 cut(s) 11, 413
StyD4I CCNGG 2 cut(s) 810, 847
StyI CCWWGG 2 cut(s) 897, 996
TaaI ACNGT 7 cut(s) 42, 135, 167, 352, 511, 539, 669
TaiI ACGT 2 cut(s) 144, 1058
TaqI TCGA 4 cut(s) 138, 546, 553, 724
TasI AATT 7 cut(s) 194, 421, 468, 569, 636, 681, 1005
TauI GCSGC 1 cut(s) 659
Tru1I TTAA 6 cut(s) 128, 624, 635, 684, 866, 878
Tru9I TTAA 6 cut(s) 128, 624, 635, 684, 866, 878
TseI GCWGC 3 cut(s) 121, 752, 767
TspDTI ATGAA 2 cut(s) 379, 480
TspGWI ACGGA 3 cut(s) 76, 317, 580
Van91I CCANNNNNTGG 1 cut(s) 826
VneI GTGCAC 1 cut(s) 233
VpaK11BI GGWCC 1 cut(s) 337
XapI RAATTY 1 cut(s) 421
XhoI CTCGAG 1 cut(s) 545
XmnI GAANNNNTTC 1 cut(s) 1016
XspI CTAG 2 cut(s) 11, 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.