MD03G1247500.v1.1

NADH kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007259
Physical Location & Seq
Forward (+)
33490192 .. 33492965
2774 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1247500.v1.1.491

Sequence Viewer

Length: 810 bp
ATGGCGAGGAGGAGACTGTTGCTGCTGTTAAAACCGTTCGACGTTTACCCAGTCGCCCAATCAGACTGTCTATCTCGCATCACCAACCCTCAGATACTACATTACTTGGAGAACAGGCAAAAGGTGCACAAGGATGCAATAAACTTTTGTCAGAATATTTTGCGCCAGAAGCCTGTTGAGTGGAAACCTACAGTACGTAGCAATCTATCAGAGCCTATCCGTGATGTGGACCTTGTTGTTACAGTTGGGGGTGATGGCACTCTATTACAAGGTAGCCATTTCATTGATGACTCGGTTCCAGTTCTAGGAGTGAATTCTGACCCCACACAACCTGAGGAGGTGGAGAAGCTCAGGAATGAGTTTGATGCTAATAGAAGCAGAGGCTATCTTTGCAGTGCAACAGTGCTGGACAACATCCTCGAGAGTCAAGCTAATCCGTCCAAATTGACGAGGATATCAATACATGTTAACTCAGAAGTGCTTCCAACTTATGCTCTTAATGATATTTTAACTTCACATCCATGTCCGGCAGCACTTTCGCGATTGTCATTCAAAATTAAAAAGAATGACCAGCCATGTTCCCTGCTGGTGCATTCTCGATCAAGTGGTCCCAGAGTTTCAACAGCTGCTGGATCAACTGCTGCAATGCTCTCAGCAGGCGGATTTCCGATGCCCATCTTATCTCAGGATCTCCAGTATATGGTAAGAGAGCCCATTTCGCCAGGCAATGCCTCGAGCTTAATGCACGGATTAATCAAATCTCATCAGTCCATGGAAGCGACGTGGTTGTCTAAAGAGGGGGTCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

270

Amino Acids

29.67

Weight (kDa)

8.27

Isoelectric Point (pI)

60.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_kinase PF01513 64 - 105 7.8e-08 ATP-NAD kinase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 787
AccB7I CCANNNNNTGG 1 cut(s) 700
AccII CGCG 1 cut(s) 541
AciI CCGC 1 cut(s) 660
AclWI GGATC 2 cut(s) 640, 696
AcsI RAATTY 1 cut(s) 313
AfaI GTAC 1 cut(s) 195
AfiI CCNNNNNNNGG 3 cut(s) 226, 305, 700
AflIII ACRYGT 1 cut(s) 463
AgsI TTSAA 2 cut(s) 553, 621
AjiI CACGTC 1 cut(s) 783
AjnI CCWGG 1 cut(s) 721
AluBI AGCT 4 cut(s) 349, 431, 626, 738
AluI AGCT 4 cut(s) 349, 431, 626, 738
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 1 cut(s) 7
Alw44I GTGCAC 1 cut(s) 125
AlwI GGATC 2 cut(s) 640, 696
AlwNI CAGNNNCTG 1 cut(s) 629
Ama87I CYCGRG 2 cut(s) 419, 733
ApaLI GTGCAC 1 cut(s) 125
ApeKI GCWGC 4 cut(s) 22, 530, 626, 641
ApoI RAATTY 1 cut(s) 313
AseI ATTAAT 1 cut(s) 752
Asp700I GAANNNNTTC 1 cut(s) 480
AspLEI GCGC 1 cut(s) 165
AspS9I GGNCC 2 cut(s) 229, 608
AsuHPI GGTGA 2 cut(s) 73, 263
AvaI CYCGRG 2 cut(s) 419, 733
AvaII GGWCC 2 cut(s) 229, 608
AxyI CCTNAGG 1 cut(s) 333
BaeGI GKGCMC 1 cut(s) 129
BanII GRGCYC 1 cut(s) 714
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 4 cut(s) 9, 542, 613, 628
BccI CCATC 2 cut(s) 248, 683
BcgI CGANNNNNNTGC 4 cut(s) 519, 553, 724, 758
BciT130I CCWGG 1 cut(s) 723
BcoDI GTCTC 1 cut(s) 7
BfaI CTAG 1 cut(s) 305
BfmI CTRYAG 1 cut(s) 189
BisI GCNGC 4 cut(s) 23, 531, 627, 642
BlsI GCNGC 4 cut(s) 24, 532, 628, 643
Bme1390I CCNGG 1 cut(s) 723
Bme18I GGWCC 2 cut(s) 229, 608
BmeT110I CYCGRG 2 cut(s) 419, 733
BmgBI CACGTC 1 cut(s) 783
BmgT120I GGNCC 2 cut(s) 229, 608
BmiI GGNNCC 2 cut(s) 297, 610
BmrFI CCNGG 1 cut(s) 723
BmrI ACTGGG 1 cut(s) 44
BmsI GCATC 4 cut(s) 87, 124, 355, 660
BmuI ACTGGG 1 cut(s) 44
BpmI CTGGAG 1 cut(s) 677
Bpu10I CCTNAGC 1 cut(s) 350
BsaAI YACGTR 1 cut(s) 197
BsaBI GATNNNNATC 1 cut(s) 674
BsaJI CCNNGG 1 cut(s) 771
Bsc4I CCNNNNNNNGG 3 cut(s) 226, 305, 700
Bse1I ACTGG 3 cut(s) 50, 299, 694
Bse21I CCTNAGG 1 cut(s) 333
Bse3DI GCAATG 2 cut(s) 651, 733
Bse8I GATNNNNATC 1 cut(s) 674
BseBI CCWGG 1 cut(s) 723
BseDI CCNNGG 1 cut(s) 771
BseGI GGATG 3 cut(s) 139, 414, 517
BseJI GATNNNNATC 1 cut(s) 674
BseLI CCNNNNNNNGG 3 cut(s) 226, 305, 700
BseMI GCAATG 2 cut(s) 651, 733
BseMII CTCAG 6 cut(s) 104, 324, 364, 486, 666, 698
BseNI ACTGG 3 cut(s) 50, 299, 694
BseRI GAGGAG 3 cut(s) 22, 25, 350
BseSI GKGCMC 1 cut(s) 129
BseXI GCAGC 4 cut(s) 9, 542, 613, 628
Bsh1236I CGCG 1 cut(s) 541
BsiHKAI GWGCWC 1 cut(s) 129
BsiHKCI CYCGRG 2 cut(s) 419, 733
BsiSI CCGG 1 cut(s) 527
BslFI GGGAC 1 cut(s) 594
BslI CCNNNNNNNGG 3 cut(s) 226, 305, 700
BsmAI GTCTC 1 cut(s) 7
BsmFI GGGAC 1 cut(s) 594
BsmI GAATGC 1 cut(s) 592
BsoBI CYCGRG 2 cut(s) 419, 733
Bsp1286I GDGCHC 2 cut(s) 129, 714
Bsp143I GATC 3 cut(s) 599, 632, 688
Bsp19I CCATGG 1 cut(s) 771
Bsp68I TCGCGA 1 cut(s) 541
BspACI CCGC 1 cut(s) 660
BspCNI CTCAG 6 cut(s) 103, 325, 363, 485, 665, 697
BspFNI CGCG 1 cut(s) 541
BspLI GGNNCC 2 cut(s) 297, 610
BspPI GGATC 2 cut(s) 640, 696
BsrDI GCAATG 2 cut(s) 651, 733
BsrI ACTGG 3 cut(s) 50, 299, 694
BssECI CCNNGG 1 cut(s) 771
BssMI GATC 3 cut(s) 599, 632, 688
BssT1I CCWWGG 1 cut(s) 771
Bst2UI CCWGG 1 cut(s) 723
Bst4CI ACNGT 6 cut(s) 18, 36, 68, 193, 244, 403
BstAPI GCANNNNNTGC 1 cut(s) 124
BstBAI YACGTR 1 cut(s) 197
BstC8I GCNNGC 1 cut(s) 658
BstDEI CTNAG 6 cut(s) 90, 333, 350, 472, 652, 684
BstDSI CCRYGG 1 cut(s) 771
BstF5I GGATG 3 cut(s) 139, 414, 517
BstFNI CGCG 1 cut(s) 541
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 3 cut(s) 602, 635, 691
BstMAI GTCTC 1 cut(s) 7
BstMBI GATC 3 cut(s) 599, 632, 688
BstMWI GCNNNNNNNGC 4 cut(s) 124, 169, 390, 718
BstNI CCWGG 1 cut(s) 723
BstNSI RCATGY 1 cut(s) 467
BstSCI CCNGG 1 cut(s) 721
BstSFI CTRYAG 1 cut(s) 189
BstSLI GKGCMC 1 cut(s) 129
BstSNI TACGTA 1 cut(s) 197
BstUI CGCG 1 cut(s) 541
BstV1I GCAGC 4 cut(s) 9, 542, 613, 628
BstX2I RGATCY 1 cut(s) 688
BstYI RGATCY 1 cut(s) 688
Bsu36I CCTNAGG 1 cut(s) 333
BtgI CCRYGG 1 cut(s) 771
BtrI CACGTC 1 cut(s) 783
BtsCI GGATG 3 cut(s) 139, 414, 517
BtsI GCAGTG 1 cut(s) 400
BtsIMutI CAGTG 2 cut(s) 400, 408
BtuMI TCGCGA 1 cut(s) 541
Cac8I GCNNGC 1 cut(s) 658
CaiI CAGNNNCTG 1 cut(s) 629
CfoI GCGC 1 cut(s) 165
Cfr13I GGNCC 2 cut(s) 229, 608
Csp6I GTAC 1 cut(s) 194
CviAII CATG 4 cut(s) 464, 522, 576, 772
CviQI GTAC 1 cut(s) 194
DdeI CTNAG 6 cut(s) 90, 333, 350, 472, 652, 684
DpnI GATC 3 cut(s) 601, 634, 690
DpnII GATC 3 cut(s) 599, 632, 688
DrdI GACNNNNNNGTC 1 cut(s) 787
DseDI GACNNNNNNGTC 1 cut(s) 787
EciI GGCGGA 1 cut(s) 675
Eco105I TACGTA 1 cut(s) 197
Eco130I CCWWGG 1 cut(s) 771
Eco24I GRGCYC 1 cut(s) 714
Eco32I GATATC 1 cut(s) 456
Eco47I GGWCC 2 cut(s) 229, 608
Eco81I CCTNAGG 1 cut(s) 333
Eco88I CYCGRG 2 cut(s) 419, 733
EcoRI GAATTC 1 cut(s) 313
EcoRII CCWGG 1 cut(s) 721
EcoRV GATATC 1 cut(s) 456
EcoT14I CCWWGG 1 cut(s) 771
EcoT38I GRGCYC 1 cut(s) 714
ErhI CCWWGG 1 cut(s) 771
FaeI CATG 4 cut(s) 467, 525, 579, 775
FaiI YATR 9 cut(s) 465, 492, 523, 577, 699, 701, 773, 806, 808
FaqI GGGAC 1 cut(s) 594
FatI CATG 4 cut(s) 463, 521, 575, 771
Fnu4HI GCNGC 4 cut(s) 23, 531, 627, 642
FokI GGATG 3 cut(s) 146, 401, 504
FriOI GRGCYC 1 cut(s) 714
Fsp4HI GCNGC 4 cut(s) 23, 531, 627, 642
FspBI CTAG 1 cut(s) 305
GlaI GCGC 1 cut(s) 164
GluI GCNGC 4 cut(s) 23, 531, 627, 642
GsuI CTGGAG 1 cut(s) 677
HapII CCGG 1 cut(s) 527
HhaI GCGC 1 cut(s) 165
Hin1II CATG 4 cut(s) 467, 525, 579, 775
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HincII GTYRAC 1 cut(s) 469
HindII GTYRAC 1 cut(s) 469
HinfI GANTC 2 cut(s) 290, 424
HpaI GTTAAC 1 cut(s) 469
HpaII CCGG 1 cut(s) 527
HphI GGTGA 2 cut(s) 73, 263
Hpy166II GTNNAC 4 cut(s) 46, 127, 229, 469
Hpy188I TCNGA 7 cut(s) 64, 93, 153, 211, 319, 475, 669
Hpy188III TCNNGA 5 cut(s) 352, 421, 540, 597, 686
Hpy8I GTNNAC 4 cut(s) 46, 127, 229, 469
Hpy99I CGWCG 2 cut(s) 44, 784
HpyCH4III ACNGT 6 cut(s) 18, 36, 68, 193, 244, 403
HpyCH4IV ACGT 3 cut(s) 42, 196, 782
HpyCH4V TGCA 7 cut(s) 127, 137, 393, 398, 592, 644, 745
HpyF10VI GCNNNNNNNGC 4 cut(s) 124, 169, 390, 718
HpyF3I CTNAG 6 cut(s) 90, 333, 350, 472, 652, 684
HpySE526I ACGT 3 cut(s) 42, 196, 782
Hsp92II CATG 4 cut(s) 467, 525, 579, 775
HspAI GCGC 1 cut(s) 163
KspAI GTTAAC 1 cut(s) 469
Kzo9I GATC 3 cut(s) 599, 632, 688
Lsp1109I GCAGC 4 cut(s) 9, 542, 613, 628
LweI GCATC 4 cut(s) 87, 124, 355, 660
MaeI CTAG 1 cut(s) 305
MaeII ACGT 3 cut(s) 42, 196, 782
MaeIII GTNAC 1 cut(s) 238
MalI GATC 3 cut(s) 601, 634, 690
MboI GATC 3 cut(s) 599, 632, 688
MflI RGATCY 1 cut(s) 688
MhlI GDGCHC 2 cut(s) 129, 714
MluCI AATT 3 cut(s) 313, 443, 555
MlyI GAGTC 2 cut(s) 284, 433
MmeI TCCRAC 1 cut(s) 509
MnlI CCTC 9 cut(s) 3, 99, 328, 331, 374, 428, 444, 742, 790
MroXI GAANNNNTTC 1 cut(s) 480
MseI TTAA 7 cut(s) 29, 468, 498, 509, 558, 740, 752
MslI CAYNNNNRTG 2 cut(s) 132, 520
MspA1I CMGCKG 1 cut(s) 626
MspI CCGG 1 cut(s) 527
MspR9I CCNGG 1 cut(s) 723
Mva1269I GAATGC 1 cut(s) 592
MvaI CCWGG 1 cut(s) 723
MvnI CGCG 1 cut(s) 541
MwoI GCNNNNNNNGC 4 cut(s) 124, 169, 390, 718
NcoI CCATGG 1 cut(s) 771
NdeII GATC 3 cut(s) 599, 632, 688
NlaIII CATG 4 cut(s) 467, 525, 579, 775
NlaIV GGNNCC 2 cut(s) 297, 610
NruI TCGCGA 1 cut(s) 541
NspI RCATGY 1 cut(s) 467
PaeR7I CTCGAG 2 cut(s) 419, 733
PciI ACATGT 1 cut(s) 463
PctI GAATGC 1 cut(s) 592
PdmI GAANNNNTTC 1 cut(s) 480
PflMI CCANNNNNTGG 1 cut(s) 700
PkrI GCNGC 4 cut(s) 24, 532, 628, 643
PleI GAGTC 2 cut(s) 284, 432
PpsI GAGTC 2 cut(s) 284, 432
Ppu21I YACGTR 1 cut(s) 197
PscI ACATGT 1 cut(s) 463
PshBI ATTAAT 1 cut(s) 752
Psp6I CCWGG 1 cut(s) 721
PspGI CCWGG 1 cut(s) 721
PspN4I GGNNCC 2 cut(s) 297, 610
PspPI GGNCC 2 cut(s) 229, 608
PspXI VCTCGAGB 1 cut(s) 733
PstNI CAGNNNCTG 1 cut(s) 629
PsuI RGATCY 1 cut(s) 688
PvuII CAGCTG 1 cut(s) 626
RruI TCGCGA 1 cut(s) 541
RsaI GTAC 1 cut(s) 195
RsaNI GTAC 1 cut(s) 194
RseI CAYNNNNRTG 2 cut(s) 132, 520
SaqAI TTAA 7 cut(s) 29, 468, 498, 509, 558, 740, 752
SatI GCNGC 4 cut(s) 23, 531, 627, 642
Sau3AI GATC 3 cut(s) 599, 632, 688
Sau96I GGNCC 2 cut(s) 229, 608
SchI GAGTC 2 cut(s) 284, 433
ScrFI CCNGG 1 cut(s) 723
SduI GDGCHC 2 cut(s) 129, 714
SfaNI GCATC 4 cut(s) 87, 124, 355, 660
SfcI CTRYAG 1 cut(s) 189
Sfr274I CTCGAG 2 cut(s) 419, 733
SinI GGWCC 2 cut(s) 229, 608
SlaI CTCGAG 2 cut(s) 419, 733
SmiMI CAYNNNNRTG 2 cut(s) 132, 520
SmlI CTYRAG 2 cut(s) 419, 733
SmoI CTYRAG 2 cut(s) 419, 733
SnaBI TACGTA 1 cut(s) 197
Sse9I AATT 3 cut(s) 313, 443, 555
SsiI CCGC 1 cut(s) 660
SspI AATATT 1 cut(s) 157
SspMI CTAG 1 cut(s) 305
StyD4I CCNGG 1 cut(s) 721
StyI CCWWGG 1 cut(s) 771
TaaI ACNGT 6 cut(s) 18, 36, 68, 193, 244, 403
TaiI ACGT 3 cut(s) 45, 199, 785
TaqI TCGA 4 cut(s) 39, 420, 598, 734
TasI AATT 3 cut(s) 313, 443, 555
Tru1I TTAA 7 cut(s) 29, 468, 498, 509, 558, 740, 752
Tru9I TTAA 7 cut(s) 29, 468, 498, 509, 558, 740, 752
TscAI CASTG 2 cut(s) 400, 408
TseI GCWGC 4 cut(s) 22, 530, 626, 641
TspDTI ATGAA 1 cut(s) 271
TspGWI ACGGA 3 cut(s) 209, 426, 762
TspRI CASTG 2 cut(s) 400, 408
Van91I CCANNNNNTGG 1 cut(s) 700
VneI GTGCAC 1 cut(s) 125
VpaK11BI GGWCC 2 cut(s) 229, 608
VspI ATTAAT 1 cut(s) 752
XapI RAATTY 1 cut(s) 313
XceI RCATGY 1 cut(s) 467
XhoI CTCGAG 2 cut(s) 419, 733
XmnI GAANNNNTTC 1 cut(s) 480
XspI CTAG 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.