MD11G1270100.v1.1

NADH kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007259
Physical Location & Seq
Reverse (-)
38534697 .. 38537061
2365 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1270100.v1.1.491

Sequence Viewer

Length: 966 bp
ATGGGGAGGAAGCGATTGTTGGTGGTGCTAAAACCGATCGAAGCTGACCCAGTTTCCCAATCAGAAGGTCCATCTCGCATCACGAATCCCCAGTCTCAGATGCTACATCACTTAGACAGCAGAAGAAAGGTGCACAATGATGCAATAAGTTGTTGTCAGAATATTTTGCTCCGCAAGCCCGTTGAGTGGAATTCTGTAGTGCTTAATGATCTATCACAGCCCGTCCATGATGTGGACCTTGTTGTCACAGTAGGCGGCGATGGTACTCTATTACAAGCTAGCCATTTCATTGATGACTCAGTTCCAGTTCTAGGAGTGAATTCTGACCCCACACAGCCCAAAGAGGTAGAGAAGCTCAGCAATGAGATTGATGCTTCTAGAAGCACAGGATATCTATGCGCCACAACTATTGACAACTTCGAAGAGGTACTTGACAACATCCTCCAGGGTCGAACTATCCCTTCCAAATTGACAAGGATATCCGTATATGTAAACTCGCAAGTGCTTCCAACTTATGCCCTTAACGATATCTTGATTGCACATTCATGTCCTGCAGCACTTTCTCAGTTCTCATTCAAAATTAAAAGGGACGGCGAGTTATGTTCCCCCTTAGTGAACTCTCGGTCAAGTGGCCTCAGGGTTTCAACAGCTGCCGGATCGACAGCCGCAATGCTCTCAGCAGGCGGATTTCCGATGCCAATCTTATCTCAGGATCTCCAGTTTATGGTACGAGAGCCCATTTCACCGGGAAACACCTCACGCTTCATGCATGGATTAATCAAATCACATCAGTCCATGGAAGTTACATGGTTTTGTAAAGAAGGTGTCGTATACATCGACGGTTCTCATGTTTTCTTTTCTATACAGAATGGGGATGCCATTGAGATATCTTCGAAGGCTCCAATTTTGAAGGTTTTCTTGTCTCACTATTTTCAGACAACAGTAAAGAACTGTTGTAAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

322

Amino Acids

35.25

Weight (kDa)

6.55

Isoelectric Point (pI)

55.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_kinase PF01513 75 - 107 9.2e-09 ATP-NAD kinase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 242
AccB7I CCANNNNNTGG 2 cut(s) 232, 724
AccI GTMKAC 1 cut(s) 831
AciI CCGC 4 cut(s) 172, 255, 666, 684
AclWI GGATC 2 cut(s) 664, 720
AcsI RAATTY 2 cut(s) 190, 319
AfaI GTAC 4 cut(s) 265, 429, 729, 962
AfiI CCNNNNNNNGG 4 cut(s) 186, 232, 311, 724
AgsI TTSAA 3 cut(s) 577, 645, 910
AjnI CCWGG 1 cut(s) 444
AjuI GAANNNNNNNTTGG 1 cut(s) 34
AluBI AGCT 4 cut(s) 44, 278, 355, 650
AluI AGCT 4 cut(s) 44, 278, 355, 650
Alw21I GWGCWC 1 cut(s) 135
Alw26I GTCTC 2 cut(s) 99, 927
Alw44I GTGCAC 1 cut(s) 131
AlwI GGATC 2 cut(s) 664, 720
AoxI GGCC 1 cut(s) 631
ApaLI GTGCAC 1 cut(s) 131
ApeKI GCWGC 2 cut(s) 554, 650
ApoI RAATTY 2 cut(s) 190, 319
ArsI GACNNNNNNTTYG 2 cut(s) 77, 109
AseI ATTAAT 1 cut(s) 776
Asp700I GAANNNNTTC 1 cut(s) 914
AspLEI GCGC 1 cut(s) 401
AspS9I GGNCC 2 cut(s) 68, 235
AsuC2I CCSGG 1 cut(s) 747
AsuHPI GGTGA 1 cut(s) 735
AsuII TTCGAA 2 cut(s) 420, 893
AsuNHI GCTAGC 1 cut(s) 278
AvaII GGWCC 2 cut(s) 68, 235
AxyI CCTNAGG 1 cut(s) 635
BaeGI GKGCMC 1 cut(s) 135
BanII GRGCYC 1 cut(s) 738
Bbv12I GWGCWC 1 cut(s) 135
BbvI GCAGC 2 cut(s) 566, 637
BccI CCATC 2 cut(s) 79, 254
BceAI ACGGC 1 cut(s) 607
BciT130I CCWGG 1 cut(s) 446
BcnI CCSGG 1 cut(s) 747
BcoDI GTCTC 2 cut(s) 99, 927
BfaI CTAG 4 cut(s) 279, 311, 378, 964
BfmI CTRYAG 2 cut(s) 195, 552
BisI GCNGC 4 cut(s) 256, 555, 651, 666
BlpI GCTNAGC 1 cut(s) 356
BlsI GCNGC 4 cut(s) 257, 556, 652, 667
BmcAI AGTACT 1 cut(s) 962
Bme1390I CCNGG 2 cut(s) 446, 747
Bme18I GGWCC 2 cut(s) 68, 235
BmgT120I GGNCC 2 cut(s) 68, 235
BmiI GGNNCC 1 cut(s) 900
BmrFI CCNGG 2 cut(s) 446, 747
BmrI ACTGGG 2 cut(s) 44, 85
BmsI GCATC 6 cut(s) 87, 90, 130, 361, 684, 865
BmtI GCTAGC 1 cut(s) 282
BmuI ACTGGG 2 cut(s) 44, 85
BpmI CTGGAG 2 cut(s) 428, 701
Bpu1102I GCTNAGC 1 cut(s) 356
Bpu14I TTCGAA 2 cut(s) 420, 893
BpuMI CCSGG 1 cut(s) 747
BsaBI GATNNNNATC 1 cut(s) 698
BsaJI CCNNGG 2 cut(s) 445, 795
Bsc4I CCNNNNNNNGG 4 cut(s) 186, 232, 311, 724
Bse1I ACTGG 4 cut(s) 50, 91, 305, 718
Bse21I CCTNAGG 1 cut(s) 635
Bse3DI GCAATG 2 cut(s) 367, 675
Bse8I GATNNNNATC 1 cut(s) 698
BseBI CCWGG 1 cut(s) 446
BseDI CCNNGG 2 cut(s) 445, 795
BseGI GGATG 2 cut(s) 438, 880
BseJI GATNNNNATC 1 cut(s) 698
BseLI CCNNNNNNNGG 4 cut(s) 186, 232, 311, 724
BseMI GCAATG 2 cut(s) 367, 675
BseMII CTCAG 7 cut(s) 110, 312, 370, 578, 649, 690, 722
BseNI ACTGG 4 cut(s) 50, 91, 305, 718
BseSI GKGCMC 1 cut(s) 135
BseXI GCAGC 2 cut(s) 566, 637
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 1 cut(s) 633
BsiEI CGRYCG 1 cut(s) 39
BsiHKAI GWGCWC 1 cut(s) 135
BsiSI CCGG 2 cut(s) 654, 746
BslFI GGGAC 1 cut(s) 602
BslI CCNNNNNNNGG 4 cut(s) 186, 232, 311, 724
BsmAI GTCTC 2 cut(s) 99, 927
BsmFI GGGAC 1 cut(s) 602
BsnI GGCC 1 cut(s) 633
Bsp119I TTCGAA 2 cut(s) 420, 893
Bsp1286I GDGCHC 2 cut(s) 135, 738
Bsp143I GATC 4 cut(s) 36, 208, 656, 712
Bsp1720I GCTNAGC 1 cut(s) 356
Bsp19I CCATGG 1 cut(s) 795
BspACI CCGC 4 cut(s) 172, 255, 666, 684
BspANI GGCC 1 cut(s) 633
BspCNI CTCAG 7 cut(s) 109, 311, 369, 577, 648, 689, 721
BspLI GGNNCC 1 cut(s) 900
BspMAI CTGCAG 1 cut(s) 556
BspOI GCTAGC 1 cut(s) 282
BspPI GGATC 2 cut(s) 664, 720
BspT104I TTCGAA 2 cut(s) 420, 893
BsrDI GCAATG 2 cut(s) 367, 675
BsrI ACTGG 4 cut(s) 50, 91, 305, 718
BssECI CCNNGG 2 cut(s) 445, 795
BssMI GATC 4 cut(s) 36, 208, 656, 712
BssNAI GTATAC 1 cut(s) 832
BssT1I CCWWGG 1 cut(s) 795
Bst1107I GTATAC 1 cut(s) 832
Bst2UI CCWGG 1 cut(s) 446
Bst4CI ACNGT 4 cut(s) 250, 842, 943, 953
Bst6I CTCTTC 1 cut(s) 417
BstBI TTCGAA 2 cut(s) 420, 893
BstC8I GCNNGC 3 cut(s) 176, 280, 682
BstDEI CTNAG 9 cut(s) 96, 112, 298, 356, 564, 610, 635, 676, 708
BstDSI CCRYGG 1 cut(s) 795
BstF5I GGATG 2 cut(s) 438, 880
BstHHI GCGC 1 cut(s) 401
BstKTI GATC 4 cut(s) 39, 211, 659, 715
BstMAI GTCTC 2 cut(s) 99, 927
BstMBI GATC 4 cut(s) 36, 208, 656, 712
BstMCI CGRYCG 1 cut(s) 39
BstMWI GCNNNNNNNGC 1 cut(s) 175
BstNI CCWGG 1 cut(s) 446
BstSCI CCNGG 2 cut(s) 444, 745
BstSFI CTRYAG 2 cut(s) 195, 552
BstSLI GKGCMC 1 cut(s) 135
BstV1I GCAGC 2 cut(s) 566, 637
BstX2I RGATCY 1 cut(s) 712
BstYI RGATCY 1 cut(s) 712
BstZ17I GTATAC 1 cut(s) 832
Bsu36I CCTNAGG 1 cut(s) 635
BsuRI GGCC 1 cut(s) 633
BtgI CCRYGG 1 cut(s) 795
BtgZI GCGATG 1 cut(s) 273
BtsCI GGATG 2 cut(s) 438, 880
Cac8I GCNNGC 3 cut(s) 176, 280, 682
CfoI GCGC 1 cut(s) 401
Cfr13I GGNCC 2 cut(s) 68, 235
Csp6I GTAC 4 cut(s) 264, 428, 728, 961
CspCI CAANNNNNGTGG 2 cut(s) 391, 426
CviAII CATG 7 cut(s) 227, 546, 766, 770, 796, 807, 848
CviQI GTAC 4 cut(s) 264, 428, 728, 961
DdeI CTNAG 9 cut(s) 96, 112, 298, 356, 564, 610, 635, 676, 708
DpnI GATC 4 cut(s) 38, 210, 658, 714
DpnII GATC 4 cut(s) 36, 208, 656, 712
DrdI GACNNNNNNGTC 1 cut(s) 242
DseDI GACNNNNNNGTC 1 cut(s) 242
Eam1104I CTCTTC 1 cut(s) 417
EarI CTCTTC 1 cut(s) 417
EciI GGCGGA 1 cut(s) 699
Eco130I CCWWGG 1 cut(s) 795
Eco24I GRGCYC 1 cut(s) 738
Eco32I GATATC 4 cut(s) 392, 480, 529, 888
Eco47I GGWCC 2 cut(s) 68, 235
Eco81I CCTNAGG 1 cut(s) 635
EcoRI GAATTC 2 cut(s) 190, 319
EcoRII CCWGG 1 cut(s) 444
EcoRV GATATC 4 cut(s) 392, 480, 529, 888
EcoT14I CCWWGG 1 cut(s) 795
EcoT22I ATGCAT 1 cut(s) 771
EcoT38I GRGCYC 1 cut(s) 738
ErhI CCWWGG 1 cut(s) 795
FaeI CATG 7 cut(s) 230, 549, 769, 773, 799, 810, 851
FalI AAGNNNNNCTT 4 cut(s) 414, 446, 902, 934
FaqI GGGAC 1 cut(s) 602
FatI CATG 7 cut(s) 226, 545, 765, 769, 795, 806, 847
FblI GTMKAC 1 cut(s) 831
Fnu4HI GCNGC 4 cut(s) 256, 555, 651, 666
FokI GGATG 2 cut(s) 425, 887
FriOI GRGCYC 1 cut(s) 738
Fsp4HI GCNGC 4 cut(s) 256, 555, 651, 666
FspBI CTAG 4 cut(s) 279, 311, 378, 964
GlaI GCGC 1 cut(s) 400
GluI GCNGC 4 cut(s) 256, 555, 651, 666
GsuI CTGGAG 2 cut(s) 428, 701
HaeIII GGCC 1 cut(s) 633
HapII CCGG 2 cut(s) 654, 746
HhaI GCGC 1 cut(s) 401
Hin1II CATG 7 cut(s) 230, 549, 769, 773, 799, 810, 851
Hin6I GCGC 1 cut(s) 399
HinP1I GCGC 1 cut(s) 399
HinfI GANTC 2 cut(s) 85, 296
HpaII CCGG 2 cut(s) 654, 746
HphI GGTGA 1 cut(s) 735
Hpy166II GTNNAC 5 cut(s) 133, 235, 493, 616, 832
Hpy188I TCNGA 6 cut(s) 64, 99, 159, 325, 693, 936
Hpy188III TCNNGA 4 cut(s) 82, 378, 532, 710
Hpy8I GTNNAC 5 cut(s) 133, 235, 493, 616, 832
Hpy99I CGWCG 1 cut(s) 842
HpyAV CCTTC 5 cut(s) 59, 471, 815, 889, 904
HpyCH4III ACNGT 4 cut(s) 250, 842, 943, 953
HpyCH4V TGCA 5 cut(s) 133, 143, 539, 554, 769
HpyF10VI GCNNNNNNNGC 1 cut(s) 175
HpyF3I CTNAG 9 cut(s) 96, 112, 298, 356, 564, 610, 635, 676, 708
Hsp92II CATG 7 cut(s) 230, 549, 769, 773, 799, 810, 851
HspAI GCGC 1 cut(s) 399
Kzo9I GATC 4 cut(s) 36, 208, 656, 712
LmnI GCTCC 2 cut(s) 174, 904
Lsp1109I GCAGC 2 cut(s) 566, 637
LweI GCATC 6 cut(s) 87, 90, 130, 361, 684, 865
MaeI CTAG 4 cut(s) 279, 311, 378, 964
MaeIII GTNAC 2 cut(s) 244, 802
MalI GATC 4 cut(s) 38, 210, 658, 714
MboI GATC 4 cut(s) 36, 208, 656, 712
MboII GAAGA 3 cut(s) 135, 434, 882
MflI RGATCY 1 cut(s) 712
MhlI GDGCHC 2 cut(s) 135, 738
MluCI AATT 5 cut(s) 190, 319, 467, 579, 903
MlyI GAGTC 1 cut(s) 290
MmeI TCCRAC 1 cut(s) 533
MnlI CCTC 5 cut(s) 337, 418, 452, 644, 766
Mph1103I ATGCAT 1 cut(s) 771
MroXI GAANNNNTTC 1 cut(s) 914
MseI TTAA 4 cut(s) 204, 522, 582, 776
MslI CAYNNNNRTG 2 cut(s) 138, 544
MspA1I CMGCKG 1 cut(s) 650
MspI CCGG 2 cut(s) 654, 746
MspR9I CCNGG 2 cut(s) 446, 747
MvaI CCWGG 1 cut(s) 446
MwoI GCNNNNNNNGC 1 cut(s) 175
NciI CCSGG 1 cut(s) 747
NcoI CCATGG 1 cut(s) 795
NdeII GATC 4 cut(s) 36, 208, 656, 712
NheI GCTAGC 1 cut(s) 278
NlaIII CATG 7 cut(s) 230, 549, 769, 773, 799, 810, 851
NlaIV GGNNCC 1 cut(s) 900
NmuCI GTSAC 1 cut(s) 244
NsiI ATGCAT 1 cut(s) 771
NspV TTCGAA 2 cut(s) 420, 893
PcsI WCGNNNNNNNCGW 1 cut(s) 834
PdmI GAANNNNTTC 1 cut(s) 914
PfeI GAWTC 1 cut(s) 85
PflMI CCANNNNNTGG 2 cut(s) 232, 724
PkrI GCNGC 4 cut(s) 257, 556, 652, 667
Ple19I CGATCG 1 cut(s) 39
PleI GAGTC 1 cut(s) 290
PpsI GAGTC 1 cut(s) 290
PshBI ATTAAT 1 cut(s) 776
Psp6I CCWGG 1 cut(s) 444
PspGI CCWGG 1 cut(s) 444
PspN4I GGNNCC 1 cut(s) 900
PspPI GGNCC 2 cut(s) 68, 235
PstI CTGCAG 1 cut(s) 556
PsuI RGATCY 1 cut(s) 712
PvuI CGATCG 1 cut(s) 39
PvuII CAGCTG 1 cut(s) 650
RsaI GTAC 4 cut(s) 265, 429, 729, 962
RsaNI GTAC 4 cut(s) 264, 428, 728, 961
RseI CAYNNNNRTG 2 cut(s) 138, 544
SaqAI TTAA 4 cut(s) 204, 522, 582, 776
SatI GCNGC 4 cut(s) 256, 555, 651, 666
Sau3AI GATC 4 cut(s) 36, 208, 656, 712
Sau96I GGNCC 2 cut(s) 68, 235
ScaI AGTACT 1 cut(s) 962
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 2 cut(s) 446, 747
SduI GDGCHC 2 cut(s) 135, 738
SfaNI GCATC 6 cut(s) 87, 90, 130, 361, 684, 865
SfcI CTRYAG 2 cut(s) 195, 552
SfuI TTCGAA 2 cut(s) 420, 893
SinI GGWCC 2 cut(s) 68, 235
SmiMI CAYNNNNRTG 2 cut(s) 138, 544
Sse9I AATT 5 cut(s) 190, 319, 467, 579, 903
SsiI CCGC 4 cut(s) 172, 255, 666, 684
SspI AATATT 1 cut(s) 163
SspMI CTAG 4 cut(s) 279, 311, 378, 964
StyD4I CCNGG 2 cut(s) 444, 745
StyI CCWWGG 1 cut(s) 795
TaaI ACNGT 4 cut(s) 250, 842, 943, 953
TaqI TCGA 6 cut(s) 39, 420, 451, 659, 837, 893
TaqII GACCGA 1 cut(s) 612
TasI AATT 5 cut(s) 190, 319, 467, 579, 903
TatI WGTACW 1 cut(s) 960
TauI GCSGC 2 cut(s) 258, 668
TfiI GAWTC 1 cut(s) 85
Tru1I TTAA 4 cut(s) 204, 522, 582, 776
Tru9I TTAA 4 cut(s) 204, 522, 582, 776
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 2 cut(s) 554, 650
Tsp45I GTSAC 1 cut(s) 244
TspDTI ATGAA 3 cut(s) 277, 534, 754
TspGWI ACGGA 1 cut(s) 472
Van91I CCANNNNNTGG 2 cut(s) 232, 724
VneI GTGCAC 1 cut(s) 131
VpaK11BI GGWCC 2 cut(s) 68, 235
VspI ATTAAT 1 cut(s) 776
XapI RAATTY 2 cut(s) 190, 319
XbaI TCTAGA 1 cut(s) 377
XmiI GTMKAC 1 cut(s) 831
XmnI GAANNNNTTC 1 cut(s) 914
XspI CTAG 4 cut(s) 279, 311, 378, 964
ZrmI AGTACT 1 cut(s) 962
Zsp2I ATGCAT 1 cut(s) 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.