Rh5BG205200

NADH kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0007259
Physical Location & Seq
Forward (+)
23308290 .. 23310738
2449 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG205200.1

Sequence Viewer

Length: 945 bp
ATGGCGAGGAGGAGATTGTTGGTGCTGCTAAAACCGTTCGACGTTTACCCAGCTTCCCAATCCGACGGCCTGTCTCGCATCACCAGCCTCCAGCTACAGCACTTGGACAATAGGCGAAAGGTGCACAAGGATGCAATAAACTTTTGTCAGAATATTTTGCGGCACAAGCCTGTTGAGTGGAAAGCTCTACTGCGTAACGATATAAGGCAGCCCATCCATGATGTGGACTTTGTTATTACAGTTGGTGGTGATGGCACCTTACTACATGCTAGCCATTATATTGATGACTCTGTTCCAGTTCTAGGAGTGAATTCTGACCCTACCCGACCTGAAGAGGTGGAAAAGCACAGCAATGAGTATGATGCTAGTAGAAGCACAGGCTACCTTTGTGCTGCAACTGTTGACAACTTTGAACAGATACTGGACAACATCATAGAGGATCGAATTAGTCCTTCTGATCTGAGAAGGATCTGCATAGGTGTAAACTCACAAGTGATTTCAACTTGTGCTCTTAATGATATTTTAATTGCACATCCATGTCCAGCAACACTCTCACGGTTCTCATTCAAAATTAAAAGTGATGACCAGCCATGCTCTCCTTTAGTGAACTCTCGATCAAGCGGTCTCAGAGTTTCAACAGCTGCTGGAGCAACAGCTGCAATGCTCTCAGCTGGCGGATTTCCAATGCCCATATTATCTCAGGACCTCCAGTATATGGTAAGAGAGCCCATTACATCAGGAGCAGCCTCAAGATTAATGCATGGGTTTATCAAGCATCACCAGTCCATGGAAGCTACATGGTTTTGTAAAGAGGGAATCATATACATTGATGGTACTCATGTCTGCTGTCCTGTAAAAAATGGGGATCGCATTGAGATATCTTCCAAGGCCCCAGTTTTGAAGGTTTTCTTGCCTCACCGTGTACAGCACATTAGTGGTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

314

Amino Acids

34.82

Weight (kDa)

7.66

Isoelectric Point (pI)

63.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_kinase PF01513 68 - 104 3.9e-09 ATP-NAD kinase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 254
AccB7I CCANNNNNTGG 3 cut(s) 223, 715, 787
AciI CCGC 3 cut(s) 160, 621, 675
AclWI GGATC 3 cut(s) 447, 476, 873
AcsI RAATTY 1 cut(s) 310
AcuI CTGAAG 1 cut(s) 351
AfaI GTAC 2 cut(s) 835, 924
AfiI CCNNNNNNNGG 4 cut(s) 223, 302, 715, 787
AgsI TTSAA 5 cut(s) 413, 501, 568, 636, 901
AhdI GACNNNNNGTC 1 cut(s) 70
AleI CACNNNNGTG 1 cut(s) 933
AluBI AGCT 7 cut(s) 53, 94, 185, 641, 656, 671, 794
AluI AGCT 7 cut(s) 53, 94, 185, 641, 656, 671, 794
Alw21I GWGCWC 2 cut(s) 126, 511
Alw26I GTCTC 2 cut(s) 78, 629
Alw44I GTGCAC 1 cut(s) 122
AlwI GGATC 3 cut(s) 447, 476, 873
AlwNI CAGNNNCTG 2 cut(s) 421, 644
AoxI GGCC 2 cut(s) 67, 888
ApaLI GTGCAC 1 cut(s) 122
ApeKI GCWGC 6 cut(s) 25, 208, 392, 641, 656, 743
ApoI RAATTY 1 cut(s) 310
AseI ATTAAT 1 cut(s) 755
Asp700I GAANNNNTTC 1 cut(s) 905
AspS9I GGNCC 2 cut(s) 703, 889
AsuHPI GGTGA 4 cut(s) 73, 260, 770, 908
AsuNHI GCTAGC 1 cut(s) 269
AvaII GGWCC 1 cut(s) 703
BaeGI GKGCMC 1 cut(s) 126
BaeI ACNNNNGTAYC 2 cut(s) 825, 858
BanI GGYRCC 1 cut(s) 254
BanII GRGCYC 1 cut(s) 729
Bbv12I GWGCWC 2 cut(s) 126, 511
BbvI GCAGC 6 cut(s) 12, 220, 379, 628, 643, 755
BccI CCATC 3 cut(s) 221, 245, 824
BceAI ACGGC 1 cut(s) 82
BcoDI GTCTC 2 cut(s) 78, 629
BfaI CTAG 3 cut(s) 270, 302, 366
BfmI CTRYAG 1 cut(s) 95
BisI GCNGC 7 cut(s) 26, 161, 209, 393, 642, 657, 744
BlsI GCNGC 7 cut(s) 27, 162, 210, 394, 643, 658, 745
Bme18I GGWCC 1 cut(s) 703
BmeRI GACNNNNNGTC 1 cut(s) 70
BmgT120I GGNCC 2 cut(s) 703, 889
BmiI GGNNCC 2 cut(s) 256, 891
BmrI ACTGGG 1 cut(s) 887
BmsI GCATC 4 cut(s) 87, 121, 352, 784
BmtI GCTAGC 1 cut(s) 273
BmuI ACTGGG 1 cut(s) 887
BpmI CTGGAG 3 cut(s) 74, 666, 692
BpuEI CTTGAG 1 cut(s) 733
BsaI GGTCTC 1 cut(s) 629
BsaJI CCNNGG 2 cut(s) 786, 885
Bsc4I CCNNNNNNNGG 4 cut(s) 223, 302, 715, 787
Bse1I ACTGG 5 cut(s) 296, 426, 709, 781, 893
Bse3DI GCAATG 2 cut(s) 358, 666
BseDI CCNNGG 2 cut(s) 786, 885
BseGI GGATG 3 cut(s) 136, 213, 532
BseLI CCNNNNNNNGG 4 cut(s) 223, 302, 715, 787
BseMI GCAATG 2 cut(s) 358, 666
BseMII CTCAG 4 cut(s) 452, 640, 681, 713
BseNI ACTGG 5 cut(s) 296, 426, 709, 781, 893
BseRI GAGGAG 2 cut(s) 22, 25
BseSI GKGCMC 1 cut(s) 126
BseXI GCAGC 6 cut(s) 12, 220, 379, 628, 643, 755
BseYI CCCAGC 1 cut(s) 49
BshFI GGCC 2 cut(s) 69, 890
BshNI GGYRCC 1 cut(s) 254
BsiHKAI GWGCWC 2 cut(s) 126, 511
BslI CCNNNNNNNGG 4 cut(s) 223, 302, 715, 787
BsmAI GTCTC 2 cut(s) 78, 629
BsnI GGCC 2 cut(s) 69, 890
Bso31I GGTCTC 1 cut(s) 629
Bsp1286I GDGCHC 3 cut(s) 126, 511, 729
Bsp1407I TGTACA 1 cut(s) 922
Bsp143I GATC 5 cut(s) 439, 457, 468, 614, 865
Bsp19I CCATGG 1 cut(s) 786
BspACI CCGC 3 cut(s) 160, 621, 675
BspANI GGCC 2 cut(s) 69, 890
BspCNI CTCAG 4 cut(s) 453, 639, 680, 712
BspLI GGNNCC 2 cut(s) 256, 891
BspOI GCTAGC 1 cut(s) 273
BspPI GGATC 3 cut(s) 447, 476, 873
BspT107I GGYRCC 1 cut(s) 254
BspTNI GGTCTC 1 cut(s) 629
BsrDI GCAATG 2 cut(s) 358, 666
BsrGI TGTACA 1 cut(s) 922
BsrI ACTGG 5 cut(s) 296, 426, 709, 781, 893
BssECI CCNNGG 2 cut(s) 786, 885
BssMI GATC 5 cut(s) 439, 457, 468, 614, 865
BssT1I CCWWGG 2 cut(s) 786, 885
Bst4CI ACNGT 5 cut(s) 36, 241, 400, 558, 920
Bst6I CTCTTC 1 cut(s) 327
BstAPI GCANNNNNTGC 1 cut(s) 656
BstAUI TGTACA 1 cut(s) 922
BstC8I GCNNGC 2 cut(s) 271, 673
BstDEI CTNAG 4 cut(s) 461, 626, 667, 699
BstDSI CCRYGG 1 cut(s) 786
BstF5I GGATG 3 cut(s) 136, 213, 532
BstKTI GATC 5 cut(s) 442, 460, 471, 617, 868
BstMAI GTCTC 2 cut(s) 78, 629
BstMBI GATC 5 cut(s) 439, 457, 468, 614, 865
BstMWI GCNNNNNNNGC 6 cut(s) 75, 84, 121, 166, 647, 656
BstNSI RCATGY 1 cut(s) 269
BstSFI CTRYAG 1 cut(s) 95
BstSLI GKGCMC 1 cut(s) 126
BstV1I GCAGC 6 cut(s) 12, 220, 379, 628, 643, 755
BstX2I RGATCY 1 cut(s) 468
BstYI RGATCY 1 cut(s) 468
BsuRI GGCC 2 cut(s) 69, 890
BtgI CCRYGG 1 cut(s) 786
BtsCI GGATG 3 cut(s) 136, 213, 532
Cac8I GCNNGC 2 cut(s) 271, 673
CaiI CAGNNNCTG 2 cut(s) 421, 644
Cfr13I GGNCC 2 cut(s) 703, 889
Csp6I GTAC 2 cut(s) 834, 923
CviAII CATG 8 cut(s) 218, 266, 537, 591, 761, 787, 798, 839
CviQI GTAC 2 cut(s) 834, 923
DdeI CTNAG 4 cut(s) 461, 626, 667, 699
DpnI GATC 5 cut(s) 441, 459, 470, 616, 867
DpnII GATC 5 cut(s) 439, 457, 468, 614, 865
DriI GACNNNNNGTC 1 cut(s) 70
Eam1104I CTCTTC 1 cut(s) 327
Eam1105I GACNNNNNGTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 327
EciI GGCGGA 1 cut(s) 690
Eco130I CCWWGG 2 cut(s) 786, 885
Eco24I GRGCYC 1 cut(s) 729
Eco31I GGTCTC 1 cut(s) 629
Eco32I GATATC 1 cut(s) 879
Eco47I GGWCC 1 cut(s) 703
Eco57I CTGAAG 1 cut(s) 351
EcoO109I RGGNCCY 2 cut(s) 703, 889
EcoRI GAATTC 1 cut(s) 310
EcoRV GATATC 1 cut(s) 879
EcoT14I CCWWGG 2 cut(s) 786, 885
EcoT22I ATGCAT 1 cut(s) 762
EcoT38I GRGCYC 1 cut(s) 729
ErhI CCWWGG 2 cut(s) 786, 885
FaeI CATG 8 cut(s) 221, 269, 540, 594, 764, 790, 801, 842
FalI AAGNNNNNCTT 2 cut(s) 893, 925
FatI CATG 8 cut(s) 217, 265, 536, 590, 760, 786, 797, 838
Fnu4HI GCNGC 7 cut(s) 26, 161, 209, 393, 642, 657, 744
FokI GGATG 3 cut(s) 143, 200, 519
FriOI GRGCYC 1 cut(s) 729
Fsp4HI GCNGC 7 cut(s) 26, 161, 209, 393, 642, 657, 744
FspBI CTAG 3 cut(s) 270, 302, 366
GluI GCNGC 7 cut(s) 26, 161, 209, 393, 642, 657, 744
GsaI CCCAGC 1 cut(s) 53
GsuI CTGGAG 3 cut(s) 74, 666, 692
HaeIII GGCC 2 cut(s) 69, 890
Hin1II CATG 8 cut(s) 221, 269, 540, 594, 764, 790, 801, 842
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HinfI GANTC 2 cut(s) 287, 816
HphI GGTGA 4 cut(s) 73, 260, 770, 908
Hpy166II GTNNAC 7 cut(s) 46, 124, 226, 403, 484, 607, 923
Hpy188I TCNGA 6 cut(s) 64, 150, 316, 457, 462, 629
Hpy188III TCNNGA 4 cut(s) 612, 701, 738, 750
Hpy8I GTNNAC 7 cut(s) 46, 124, 226, 403, 484, 607, 923
Hpy99I CGWCG 2 cut(s) 44, 68
HpyAV CCTTC 3 cut(s) 459, 462, 895
HpyCH4III ACNGT 5 cut(s) 36, 241, 400, 558, 920
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 7 cut(s) 124, 134, 395, 474, 530, 659, 760
HpyF10VI GCNNNNNNNGC 6 cut(s) 75, 84, 121, 166, 647, 656
HpyF3I CTNAG 4 cut(s) 461, 626, 667, 699
HpySE526I ACGT 1 cut(s) 42
Hsp92II CATG 8 cut(s) 221, 269, 540, 594, 764, 790, 801, 842
Kzo9I GATC 5 cut(s) 439, 457, 468, 614, 865
LmnI GCTCC 2 cut(s) 647, 740
Lsp1109I GCAGC 6 cut(s) 12, 220, 379, 628, 643, 755
LweI GCATC 4 cut(s) 87, 121, 352, 784
MaeI CTAG 3 cut(s) 270, 302, 366
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 1 cut(s) 194
MalI GATC 5 cut(s) 441, 459, 470, 616, 867
MboI GATC 5 cut(s) 439, 457, 468, 614, 865
MboII GAAGA 2 cut(s) 344, 873
MflI RGATCY 1 cut(s) 468
MhlI GDGCHC 3 cut(s) 126, 511, 729
MluCI AATT 4 cut(s) 310, 444, 525, 570
MlyI GAGTC 1 cut(s) 281
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 8 cut(s) 3, 98, 328, 430, 716, 757, 805, 924
Mph1103I ATGCAT 1 cut(s) 762
MroXI GAANNNNTTC 1 cut(s) 905
MseI TTAA 4 cut(s) 513, 524, 573, 755
MslI CAYNNNNRTG 4 cut(s) 129, 351, 535, 933
MspA1I CMGCKG 3 cut(s) 641, 656, 671
MwoI GCNNNNNNNGC 6 cut(s) 75, 84, 121, 166, 647, 656
NcoI CCATGG 1 cut(s) 786
NdeII GATC 5 cut(s) 439, 457, 468, 614, 865
NheI GCTAGC 1 cut(s) 269
NlaIII CATG 8 cut(s) 221, 269, 540, 594, 764, 790, 801, 842
NlaIV GGNNCC 2 cut(s) 256, 891
NsiI ATGCAT 1 cut(s) 762
NspI RCATGY 1 cut(s) 269
OliI CACNNNNGTG 1 cut(s) 933
PdmI GAANNNNTTC 1 cut(s) 905
PfeI GAWTC 1 cut(s) 816
PflMI CCANNNNNTGG 3 cut(s) 223, 715, 787
PkrI GCNGC 7 cut(s) 27, 162, 210, 394, 643, 658, 745
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
PpuMI RGGWCCY 1 cut(s) 703
PshBI ATTAAT 1 cut(s) 755
Psp5II RGGWCCY 1 cut(s) 703
PspFI CCCAGC 1 cut(s) 49
PspN4I GGNNCC 2 cut(s) 256, 891
PspPI GGNCC 2 cut(s) 703, 889
PspPPI RGGWCCY 1 cut(s) 703
PstNI CAGNNNCTG 2 cut(s) 421, 644
PsuI RGATCY 1 cut(s) 468
PvuII CAGCTG 3 cut(s) 641, 656, 671
RsaI GTAC 2 cut(s) 835, 924
RsaNI GTAC 2 cut(s) 834, 923
RseI CAYNNNNRTG 4 cut(s) 129, 351, 535, 933
SaqAI TTAA 4 cut(s) 513, 524, 573, 755
SatI GCNGC 7 cut(s) 26, 161, 209, 393, 642, 657, 744
Sau3AI GATC 5 cut(s) 439, 457, 468, 614, 865
Sau96I GGNCC 2 cut(s) 703, 889
SchI GAGTC 1 cut(s) 281
SduI GDGCHC 3 cut(s) 126, 511, 729
SfaNI GCATC 4 cut(s) 87, 121, 352, 784
SfcI CTRYAG 1 cut(s) 95
SinI GGWCC 1 cut(s) 703
SmiMI CAYNNNNRTG 4 cut(s) 129, 351, 535, 933
SmlI CTYRAG 1 cut(s) 748
SmoI CTYRAG 1 cut(s) 748
Sse9I AATT 4 cut(s) 310, 444, 525, 570
SsiI CCGC 3 cut(s) 160, 621, 675
SspI AATATT 1 cut(s) 154
SspMI CTAG 3 cut(s) 270, 302, 366
StyI CCWWGG 2 cut(s) 786, 885
TaaI ACNGT 5 cut(s) 36, 241, 400, 558, 920
TaiI ACGT 1 cut(s) 45
TaqI TCGA 3 cut(s) 39, 442, 613
TasI AATT 4 cut(s) 310, 444, 525, 570
TatI WGTACW 1 cut(s) 922
TauI GCSGC 1 cut(s) 163
TfiI GAWTC 1 cut(s) 816
Tru1I TTAA 4 cut(s) 513, 524, 573, 755
Tru9I TTAA 4 cut(s) 513, 524, 573, 755
TseI GCWGC 6 cut(s) 25, 208, 392, 641, 656, 743
Van91I CCANNNNNTGG 3 cut(s) 223, 715, 787
VneI GTGCAC 1 cut(s) 122
VpaK11BI GGWCC 1 cut(s) 703
VspI ATTAAT 1 cut(s) 755
XapI RAATTY 1 cut(s) 310
XceI RCATGY 1 cut(s) 269
XcmI CCANNNNNNNNNTGG 1 cut(s) 220
XmnI GAANNNNTTC 1 cut(s) 905
XspI CTAG 3 cut(s) 270, 302, 366
Zsp2I ATGCAT 1 cut(s) 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.