Rroxscaffold_1G00050460

NADH kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0007259
Physical Location & Seq
Reverse (-)
70992336 .. 70994967
2632 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00050460.1

Sequence Viewer

Length: 1026 bp
ATGGCGAGGAGGAGATTGTTGGTGCTGCTAAAACCGTTCGACGTTTACCCAGCCTCCCAATCCGACGGCTTGTCTCGCATCACCAGCCTCCAGCTACAGCACTTGGACAATAGGCGAAAGGTGCACAAGGATGCAATAAACTTTTGTCAGAATATTTTGCGGCACAAGCCTGTTGAGTGGAAAGCTCTAGTGCGTAACGATCTAAGGCTGCCCATCCGTGATGTGGACCTTGTTATTACAGTTGGTGGTGATGGCACTTTACTACATGCTAGCCATTATATTGATGACTCTGTTCCAGTTCTAGGAGTGAATTCTGACCCTACCCGACCTGAGGAGGTGGAAAAGCACAGCAATGAGTATGATGCTAGTAGAAGCACAGGCTATCTTTGTGCTGCAACTGTTGACAACTTTGAACAGGCAAGACTTCTAAGATTATTTCATGAATCTGACCCAAAGAAAACCAGAATGGAGGCCAGGCTCGGAAGCCCAATGGCTCAGATTCTGGACAACATCATAGAGGATCGAATTAGTCCTTCTGATCTGAGAAGGATCTGCATAGGTGTAAACTCACAAGTGATTTCAACTTATGCTCTTAATGATATTTTAATTGCACATCCATGTCCAGCAACACTCTCACGGTTCTCATTCAAAATTAAAAGTGATGACCAGCCATGCTCTCCTTTAGTGAACTCTCGATCAAGCGGTCTCAGAGTTTCAACAGCTGCTGGAGCAACAGCTGCAATGCTCTCAGCTGGCGGATTTCCAATGCCCATATTATCTCAGGACCTCCAGTATATGGTAAGAGAGCCCATTACATCAGGAGCAGCCTCAAGATTAATGCATGGGTTTATCAAGCATCACCAGTCCATGGAAGCTACATGGTTTTGTAAAGAGGGAATCATATACATTGATGGTACTCATGTCTGCTGTTCTGTAAAAAATGGGGATCGCATTGAGATATCTTCCAAGGCCCCAGTTTTGAAGGTTTTCTTGCCTCACCGTGTACAGCACGTTAGTGGTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

341

Amino Acids

37.94

Weight (kDa)

8.75

Isoelectric Point (pI)

59.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_kinase PF01513 72 - 104 1.2e-08 ATP-NAD kinase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 796, 868
AciI CCGC 3 cut(s) 160, 702, 756
AclWI GGATC 3 cut(s) 528, 557, 954
AcsI RAATTY 1 cut(s) 310
AfaI GTAC 2 cut(s) 916, 1005
AfiI CCNNNNNNNGG 5 cut(s) 223, 302, 331, 796, 868
AgsI TTSAA 5 cut(s) 413, 582, 649, 717, 982
AhdI GACNNNNNGTC 1 cut(s) 70
AjnI CCWGG 1 cut(s) 473
AleI CACNNNNGTG 1 cut(s) 1014
AluBI AGCT 6 cut(s) 94, 185, 722, 737, 752, 875
AluI AGCT 6 cut(s) 94, 185, 722, 737, 752, 875
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 2 cut(s) 78, 710
Alw44I GTGCAC 1 cut(s) 122
AlwI GGATC 3 cut(s) 528, 557, 954
AlwNI CAGNNNCTG 2 cut(s) 502, 725
AoxI GGCC 2 cut(s) 471, 969
ApaLI GTGCAC 1 cut(s) 122
ApeKI GCWGC 6 cut(s) 25, 208, 392, 722, 737, 824
ApoI RAATTY 1 cut(s) 310
AseI ATTAAT 1 cut(s) 836
Asp700I GAANNNNTTC 1 cut(s) 986
AspS9I GGNCC 3 cut(s) 226, 784, 970
AsuHPI GGTGA 4 cut(s) 73, 260, 851, 989
AsuNHI GCTAGC 1 cut(s) 269
AvaII GGWCC 2 cut(s) 226, 784
AxyI CCTNAGG 1 cut(s) 330
BaeGI GKGCMC 1 cut(s) 126
BaeI ACNNNNGTAYC 2 cut(s) 906, 939
BanII GRGCYC 1 cut(s) 810
Bbv12I GWGCWC 1 cut(s) 126
BbvI GCAGC 6 cut(s) 12, 195, 379, 709, 724, 836
BccI CCATC 3 cut(s) 221, 245, 905
BceAI ACGGC 1 cut(s) 82
BciT130I CCWGG 1 cut(s) 475
BcoDI GTCTC 2 cut(s) 78, 710
BfaI CTAG 4 cut(s) 188, 270, 302, 366
BfmI CTRYAG 1 cut(s) 95
BisI GCNGC 7 cut(s) 26, 161, 209, 393, 723, 738, 825
BlsI GCNGC 7 cut(s) 27, 162, 210, 394, 724, 739, 826
Bme1390I CCNGG 1 cut(s) 475
Bme18I GGWCC 2 cut(s) 226, 784
BmeRI GACNNNNNGTC 1 cut(s) 70
BmgT120I GGNCC 3 cut(s) 226, 784, 970
BmiI GGNNCC 1 cut(s) 972
BmrFI CCNGG 1 cut(s) 475
BmrI ACTGGG 1 cut(s) 968
BmsI GCATC 4 cut(s) 87, 121, 352, 865
BmtI GCTAGC 1 cut(s) 273
BmuI ACTGGG 1 cut(s) 968
BpmI CTGGAG 3 cut(s) 74, 747, 773
BpuEI CTTGAG 1 cut(s) 814
BsaI GGTCTC 1 cut(s) 710
BsaJI CCNNGG 2 cut(s) 867, 966
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bsc4I CCNNNNNNNGG 5 cut(s) 223, 302, 331, 796, 868
Bse1I ACTGG 4 cut(s) 296, 790, 862, 974
Bse21I CCTNAGG 1 cut(s) 330
Bse3DI GCAATG 2 cut(s) 358, 747
BseBI CCWGG 1 cut(s) 475
BseDI CCNNGG 2 cut(s) 867, 966
BseGI GGATG 3 cut(s) 136, 213, 613
BseLI CCNNNNNNNGG 5 cut(s) 223, 302, 331, 796, 868
BseMI GCAATG 2 cut(s) 358, 747
BseMII CTCAG 6 cut(s) 321, 509, 533, 721, 762, 794
BseNI ACTGG 4 cut(s) 296, 790, 862, 974
BseRI GAGGAG 3 cut(s) 22, 25, 347
BseSI GKGCMC 1 cut(s) 126
BseXI GCAGC 6 cut(s) 12, 195, 379, 709, 724, 836
BseYI CCCAGC 1 cut(s) 49
BshFI GGCC 2 cut(s) 473, 971
BsiHKAI GWGCWC 1 cut(s) 126
BslI CCNNNNNNNGG 5 cut(s) 223, 302, 331, 796, 868
BsmAI GTCTC 2 cut(s) 78, 710
BsnI GGCC 2 cut(s) 473, 971
Bso31I GGTCTC 1 cut(s) 710
Bsp1286I GDGCHC 2 cut(s) 126, 810
Bsp1407I TGTACA 1 cut(s) 1003
Bsp143I GATC 6 cut(s) 199, 520, 538, 549, 695, 946
Bsp19I CCATGG 1 cut(s) 867
BspACI CCGC 3 cut(s) 160, 702, 756
BspANI GGCC 2 cut(s) 473, 971
BspCNI CTCAG 6 cut(s) 322, 508, 534, 720, 761, 793
BspHI TCATGA 1 cut(s) 439
BspLI GGNNCC 1 cut(s) 972
BspOI GCTAGC 1 cut(s) 273
BspPI GGATC 3 cut(s) 528, 557, 954
BspTNI GGTCTC 1 cut(s) 710
BsrDI GCAATG 2 cut(s) 358, 747
BsrGI TGTACA 1 cut(s) 1003
BsrI ACTGG 4 cut(s) 296, 790, 862, 974
BssECI CCNNGG 2 cut(s) 867, 966
BssMI GATC 6 cut(s) 199, 520, 538, 549, 695, 946
BssT1I CCWWGG 2 cut(s) 867, 966
Bst2UI CCWGG 1 cut(s) 475
Bst4CI ACNGT 5 cut(s) 36, 241, 400, 639, 1001
BstAPI GCANNNNNTGC 1 cut(s) 737
BstAUI TGTACA 1 cut(s) 1003
BstC8I GCNNGC 2 cut(s) 271, 754
BstDEI CTNAG 8 cut(s) 203, 330, 428, 495, 542, 707, 748, 780
BstDSI CCRYGG 1 cut(s) 867
BstF5I GGATG 3 cut(s) 136, 213, 613
BstKTI GATC 6 cut(s) 202, 523, 541, 552, 698, 949
BstMAI GTCTC 2 cut(s) 78, 710
BstMBI GATC 6 cut(s) 199, 520, 538, 549, 695, 946
BstMWI GCNNNNNNNGC 6 cut(s) 75, 84, 121, 166, 728, 737
BstNI CCWGG 1 cut(s) 475
BstNSI RCATGY 1 cut(s) 269
BstSCI CCNGG 1 cut(s) 473
BstSFI CTRYAG 1 cut(s) 95
BstSLI GKGCMC 1 cut(s) 126
BstV1I GCAGC 6 cut(s) 12, 195, 379, 709, 724, 836
BstX2I RGATCY 1 cut(s) 549
BstYI RGATCY 1 cut(s) 549
Bsu36I CCTNAGG 1 cut(s) 330
BsuRI GGCC 2 cut(s) 473, 971
BtgI CCRYGG 1 cut(s) 867
BtsCI GGATG 3 cut(s) 136, 213, 613
Cac8I GCNNGC 2 cut(s) 271, 754
CaiI CAGNNNCTG 2 cut(s) 502, 725
CciI TCATGA 1 cut(s) 439
Cfr13I GGNCC 3 cut(s) 226, 784, 970
Csp6I GTAC 2 cut(s) 915, 1004
CviAII CATG 8 cut(s) 266, 440, 618, 672, 842, 868, 879, 920
CviQI GTAC 2 cut(s) 915, 1004
DdeI CTNAG 8 cut(s) 203, 330, 428, 495, 542, 707, 748, 780
DpnI GATC 6 cut(s) 201, 522, 540, 551, 697, 948
DpnII GATC 6 cut(s) 199, 520, 538, 549, 695, 946
DriI GACNNNNNGTC 1 cut(s) 70
Eam1105I GACNNNNNGTC 1 cut(s) 70
EciI GGCGGA 1 cut(s) 771
Eco130I CCWWGG 2 cut(s) 867, 966
Eco24I GRGCYC 1 cut(s) 810
Eco31I GGTCTC 1 cut(s) 710
Eco32I GATATC 1 cut(s) 960
Eco47I GGWCC 2 cut(s) 226, 784
Eco81I CCTNAGG 1 cut(s) 330
EcoO109I RGGNCCY 2 cut(s) 784, 970
EcoRI GAATTC 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 473
EcoRV GATATC 1 cut(s) 960
EcoT14I CCWWGG 2 cut(s) 867, 966
EcoT22I ATGCAT 1 cut(s) 843
EcoT38I GRGCYC 1 cut(s) 810
ErhI CCWWGG 2 cut(s) 867, 966
FaeI CATG 8 cut(s) 269, 443, 621, 675, 845, 871, 882, 923
FalI AAGNNNNNCTT 2 cut(s) 974, 1006
FatI CATG 8 cut(s) 265, 439, 617, 671, 841, 867, 878, 919
Fnu4HI GCNGC 7 cut(s) 26, 161, 209, 393, 723, 738, 825
FokI GGATG 3 cut(s) 143, 200, 600
FriOI GRGCYC 1 cut(s) 810
Fsp4HI GCNGC 7 cut(s) 26, 161, 209, 393, 723, 738, 825
FspBI CTAG 4 cut(s) 188, 270, 302, 366
GluI GCNGC 7 cut(s) 26, 161, 209, 393, 723, 738, 825
GsaI CCCAGC 1 cut(s) 53
GsuI CTGGAG 3 cut(s) 74, 747, 773
HaeIII GGCC 2 cut(s) 473, 971
Hin1II CATG 8 cut(s) 269, 443, 621, 675, 845, 871, 882, 923
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HinfI GANTC 4 cut(s) 287, 443, 499, 897
HphI GGTGA 4 cut(s) 73, 260, 851, 989
Hpy166II GTNNAC 7 cut(s) 46, 124, 226, 403, 565, 688, 1004
Hpy188I TCNGA 9 cut(s) 64, 150, 316, 448, 482, 498, 538, 543, 710
Hpy188III TCNNGA 6 cut(s) 440, 503, 693, 782, 819, 831
Hpy8I GTNNAC 7 cut(s) 46, 124, 226, 403, 565, 688, 1004
Hpy99I CGWCG 2 cut(s) 44, 68
HpyAV CCTTC 3 cut(s) 540, 543, 976
HpyCH4III ACNGT 5 cut(s) 36, 241, 400, 639, 1001
HpyCH4IV ACGT 2 cut(s) 42, 1011
HpyCH4V TGCA 7 cut(s) 124, 134, 395, 555, 611, 740, 841
HpyF10VI GCNNNNNNNGC 6 cut(s) 75, 84, 121, 166, 728, 737
HpyF3I CTNAG 8 cut(s) 203, 330, 428, 495, 542, 707, 748, 780
HpySE526I ACGT 2 cut(s) 42, 1011
Hsp92II CATG 8 cut(s) 269, 443, 621, 675, 845, 871, 882, 923
Kzo9I GATC 6 cut(s) 199, 520, 538, 549, 695, 946
LmnI GCTCC 2 cut(s) 728, 821
Lsp1109I GCAGC 6 cut(s) 12, 195, 379, 709, 724, 836
LweI GCATC 4 cut(s) 87, 121, 352, 865
MaeI CTAG 4 cut(s) 188, 270, 302, 366
MaeII ACGT 2 cut(s) 42, 1011
MaeIII GTNAC 1 cut(s) 194
MalI GATC 6 cut(s) 201, 522, 540, 551, 697, 948
MboI GATC 6 cut(s) 199, 520, 538, 549, 695, 946
MboII GAAGA 1 cut(s) 954
MflI RGATCY 1 cut(s) 549
MhlI GDGCHC 2 cut(s) 126, 810
MluCI AATT 4 cut(s) 310, 525, 606, 651
MlyI GAGTC 1 cut(s) 281
MmeI TCCRAC 1 cut(s) 87
Mph1103I ATGCAT 1 cut(s) 843
MroXI GAANNNNTTC 1 cut(s) 986
MseI TTAA 4 cut(s) 594, 605, 654, 836
MslI CAYNNNNRTG 4 cut(s) 129, 351, 616, 1014
MspA1I CMGCKG 3 cut(s) 722, 737, 752
MspR9I CCNGG 1 cut(s) 475
MvaI CCWGG 1 cut(s) 475
MwoI GCNNNNNNNGC 6 cut(s) 75, 84, 121, 166, 728, 737
NcoI CCATGG 1 cut(s) 867
NdeII GATC 6 cut(s) 199, 520, 538, 549, 695, 946
NheI GCTAGC 1 cut(s) 269
NlaIII CATG 8 cut(s) 269, 443, 621, 675, 845, 871, 882, 923
NlaIV GGNNCC 1 cut(s) 972
NsiI ATGCAT 1 cut(s) 843
NspI RCATGY 1 cut(s) 269
OliI CACNNNNGTG 1 cut(s) 1014
PagI TCATGA 1 cut(s) 439
PdmI GAANNNNTTC 1 cut(s) 986
PfeI GAWTC 3 cut(s) 443, 499, 897
PflMI CCANNNNNTGG 2 cut(s) 796, 868
PkrI GCNGC 7 cut(s) 27, 162, 210, 394, 724, 739, 826
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
PpuMI RGGWCCY 1 cut(s) 784
PshBI ATTAAT 1 cut(s) 836
Psp5II RGGWCCY 1 cut(s) 784
Psp6I CCWGG 1 cut(s) 473
PspFI CCCAGC 1 cut(s) 49
PspGI CCWGG 1 cut(s) 473
PspN4I GGNNCC 1 cut(s) 972
PspPI GGNCC 3 cut(s) 226, 784, 970
PspPPI RGGWCCY 1 cut(s) 784
PstNI CAGNNNCTG 2 cut(s) 502, 725
PsuI RGATCY 1 cut(s) 549
PvuII CAGCTG 3 cut(s) 722, 737, 752
RsaI GTAC 2 cut(s) 916, 1005
RsaNI GTAC 2 cut(s) 915, 1004
RseI CAYNNNNRTG 4 cut(s) 129, 351, 616, 1014
SaqAI TTAA 4 cut(s) 594, 605, 654, 836
SatI GCNGC 7 cut(s) 26, 161, 209, 393, 723, 738, 825
Sau3AI GATC 6 cut(s) 199, 520, 538, 549, 695, 946
Sau96I GGNCC 3 cut(s) 226, 784, 970
SchI GAGTC 1 cut(s) 281
ScrFI CCNGG 1 cut(s) 475
SduI GDGCHC 2 cut(s) 126, 810
SfaNI GCATC 4 cut(s) 87, 121, 352, 865
SfcI CTRYAG 1 cut(s) 95
SinI GGWCC 2 cut(s) 226, 784
SmiMI CAYNNNNRTG 4 cut(s) 129, 351, 616, 1014
SmlI CTYRAG 1 cut(s) 829
SmoI CTYRAG 1 cut(s) 829
Sse9I AATT 4 cut(s) 310, 525, 606, 651
SsiI CCGC 3 cut(s) 160, 702, 756
SspI AATATT 1 cut(s) 154
SspMI CTAG 4 cut(s) 188, 270, 302, 366
StyD4I CCNGG 1 cut(s) 473
StyI CCWWGG 2 cut(s) 867, 966
TaaI ACNGT 5 cut(s) 36, 241, 400, 639, 1001
TaiI ACGT 2 cut(s) 45, 1014
TaqI TCGA 3 cut(s) 39, 523, 694
TasI AATT 4 cut(s) 310, 525, 606, 651
TatI WGTACW 1 cut(s) 1003
TauI GCSGC 1 cut(s) 163
TfiI GAWTC 3 cut(s) 443, 499, 897
Tru1I TTAA 4 cut(s) 594, 605, 654, 836
Tru9I TTAA 4 cut(s) 594, 605, 654, 836
TseI GCWGC 6 cut(s) 25, 208, 392, 722, 737, 824
TspDTI ATGAA 2 cut(s) 428, 456
TspGWI ACGGA 1 cut(s) 206
Van91I CCANNNNNTGG 2 cut(s) 796, 868
VneI GTGCAC 1 cut(s) 122
VpaK11BI GGWCC 2 cut(s) 226, 784
VspI ATTAAT 1 cut(s) 836
XapI RAATTY 1 cut(s) 310
XceI RCATGY 1 cut(s) 269
XcmI CCANNNNNNNNNTGG 1 cut(s) 220
XmnI GAANNNNTTC 1 cut(s) 986
XspI CTAG 4 cut(s) 188, 270, 302, 366
Zsp2I ATGCAT 1 cut(s) 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.