MD11G1270000.v1.1

NADH kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007259
Physical Location & Seq
Reverse (-)
38530757 .. 38533733
2977 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1270000.v1.1.491

Sequence Viewer

Length: 951 bp
ATGGCGAGGAGGAGACTGTTGCTGCTGTTAAAACCGTTCGACGTTTACCAAGTCGGCCAATCAGACGGTCTGTGTCGCATCACCAACCCTCAGGTACTACATTACTTGGAGAACAGGCGAAAAGTGCACAAGGATGCAATAAACTTGTGTCAGAGTATTTTGCGCCAGAAGCCTGTTGAGTGGAAACCTATAGTACGTACTAATCTATCCCAGCCTATCCGTGATGTGGACCTTGTTGTTACAGTTGGGGGTGATGGCACTCTATTACAAGGCAGCCATTTCATTGATGACACGGTTCCAGTTCTAGGAGTGAATTCTGACCCCACACAACCTGAAGAGGTAGAGAAGCTCAGGAATGAGTTTGATGCTAATAGAAGCACAGGCTATCTTTGCGGTGCAACGGTCAACAACTTTGAACAGGTTCTGGACAATATCCTCGAGGGTCGAGCTAATCCTTCCAAATTGACAAGGATATCCATATGTGTAAACTCAGAAGTGATTCCAACTTATGCTCTTAATGATATTTTAATTGCACATCCATGTCCGGCAGCAGTTTCACGATTCTCATTCAAAATTAAAATGGATGACCAGCCATGTTCCCCACTGGTGCATTCTCGATCAAGTGGTCTCAGAGTTTCAACAGCTGCTGGATCAACCGCTGCAATGCTCTCAGCAGGCGGATTTCCGATGCCCATCTTATCTCAGGATCTCCAGTACATGGTAAGAGAGCCCATTTCACCAGGAAACACCTCAAGCTTAATGCACGGATCAATCAAATCTCGTCAGTCCATGGAAGCTACGTGGTTGTCTAAAGAGGGCGTCGTATACATTGACGGTTCTCATGTTTGCTATTCTATAAAGGATGGCGATGTCATAGAGATATCTCCCAAGGCCCCAGTTTTGAAGGTTTTTTTGCCTCACCATTTACAGAACATTAGTGAAGAAACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

317

Amino Acids

34.87

Weight (kDa)

6.75

Isoelectric Point (pI)

56.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_kinase PF01513 73 - 105 2.3e-07 ATP-NAD kinase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 437
AccB7I CCANNNNNTGG 1 cut(s) 718
AccI GTMKAC 1 cut(s) 825
AciI CCGC 3 cut(s) 393, 657, 678
AclWI GGATC 3 cut(s) 658, 714, 775
AcoI YGGCCR 1 cut(s) 55
AcsI RAATTY 1 cut(s) 313
AcuI CTGAAG 1 cut(s) 354
AcyI GRCGYC 1 cut(s) 819
AfaI GTAC 4 cut(s) 96, 195, 199, 716
AfiI CCNNNNNNNGG 3 cut(s) 226, 305, 718
AgsI TTSAA 4 cut(s) 416, 571, 639, 904
AjnI CCWGG 1 cut(s) 739
AluBI AGCT 5 cut(s) 349, 449, 644, 756, 797
AluI AGCT 5 cut(s) 349, 449, 644, 756, 797
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 2 cut(s) 7, 632
Alw44I GTGCAC 1 cut(s) 125
AlwI GGATC 3 cut(s) 658, 714, 775
AlwNI CAGNNNCTG 2 cut(s) 424, 647
Ama87I CYCGRG 1 cut(s) 437
AoxI GGCC 2 cut(s) 55, 891
ApaLI GTGCAC 1 cut(s) 125
ApeKI GCWGC 5 cut(s) 22, 273, 548, 644, 659
ApoI RAATTY 1 cut(s) 313
Asp700I GAANNNNTTC 2 cut(s) 420, 498
AspLEI GCGC 1 cut(s) 165
AspS9I GGNCC 2 cut(s) 229, 892
AsuHPI GGTGA 4 cut(s) 73, 263, 729, 911
AvaI CYCGRG 1 cut(s) 437
AvaII GGWCC 1 cut(s) 229
AxyI CCTNAGG 1 cut(s) 90
BaeGI GKGCMC 1 cut(s) 129
BanII GRGCYC 1 cut(s) 732
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 5 cut(s) 9, 285, 560, 631, 646
BccI CCATC 3 cut(s) 248, 701, 857
BciT130I CCWGG 1 cut(s) 741
BcoDI GTCTC 2 cut(s) 7, 632
BfaI CTAG 1 cut(s) 305
BfmI CTRYAG 1 cut(s) 189
BisI GCNGC 5 cut(s) 23, 274, 549, 645, 660
BlsI GCNGC 5 cut(s) 24, 275, 550, 646, 661
Bme1390I CCNGG 1 cut(s) 741
Bme18I GGWCC 1 cut(s) 229
BmeT110I CYCGRG 1 cut(s) 437
BmgT120I GGNCC 2 cut(s) 229, 892
BmiI GGNNCC 2 cut(s) 297, 894
BmrFI CCNGG 1 cut(s) 741
BmrI ACTGGG 1 cut(s) 890
BmsI GCATC 4 cut(s) 87, 124, 355, 678
BmuI ACTGGG 1 cut(s) 890
BpmI CTGGAG 1 cut(s) 695
Bpu10I CCTNAGC 1 cut(s) 350
BpuEI CTTGAG 1 cut(s) 736
BsaAI YACGTR 2 cut(s) 197, 801
BsaBI GATNNNNATC 1 cut(s) 692
BsaHI GRCGYC 1 cut(s) 819
BsaI GGTCTC 1 cut(s) 632
BsaJI CCNNGG 2 cut(s) 789, 888
Bsc4I CCNNNNNNNGG 3 cut(s) 226, 305, 718
Bse1I ACTGG 4 cut(s) 299, 609, 712, 896
Bse21I CCTNAGG 1 cut(s) 90
Bse3DI GCAATG 1 cut(s) 669
Bse8I GATNNNNATC 1 cut(s) 692
BseBI CCWGG 1 cut(s) 741
BseDI CCNNGG 2 cut(s) 789, 888
BseGI GGATG 4 cut(s) 139, 535, 589, 868
BseJI GATNNNNATC 1 cut(s) 692
BseLI CCNNNNNNNGG 3 cut(s) 226, 305, 718
BseMI GCAATG 1 cut(s) 669
BseMII CTCAG 6 cut(s) 104, 364, 504, 643, 684, 716
BseNI ACTGG 4 cut(s) 299, 609, 712, 896
BseRI GAGGAG 2 cut(s) 22, 25
BseSI GKGCMC 1 cut(s) 129
BseXI GCAGC 5 cut(s) 9, 285, 560, 631, 646
BseYI CCCAGC 1 cut(s) 210
BshFI GGCC 2 cut(s) 57, 893
BsiHKAI GWGCWC 1 cut(s) 129
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 1 cut(s) 545
BslI CCNNNNNNNGG 3 cut(s) 226, 305, 718
BsmAI GTCTC 2 cut(s) 7, 632
BsmI GAATGC 1 cut(s) 610
BsnI GGCC 2 cut(s) 57, 893
Bso31I GGTCTC 1 cut(s) 632
BsoBI CYCGRG 1 cut(s) 437
Bsp1286I GDGCHC 2 cut(s) 129, 732
Bsp143I GATC 4 cut(s) 617, 650, 706, 767
Bsp19I CCATGG 1 cut(s) 789
BspACI CCGC 3 cut(s) 393, 657, 678
BspANI GGCC 2 cut(s) 57, 893
BspCNI CTCAG 6 cut(s) 103, 363, 503, 642, 683, 715
BspLI GGNNCC 2 cut(s) 297, 894
BspPI GGATC 3 cut(s) 658, 714, 775
BspTNI GGTCTC 1 cut(s) 632
BsrDI GCAATG 1 cut(s) 669
BsrI ACTGG 4 cut(s) 299, 609, 712, 896
BssECI CCNNGG 2 cut(s) 789, 888
BssMI GATC 4 cut(s) 617, 650, 706, 767
BssNAI GTATAC 1 cut(s) 826
BssNI GRCGYC 1 cut(s) 819
BssT1I CCWWGG 2 cut(s) 789, 888
Bst1107I GTATAC 1 cut(s) 826
Bst2UI CCWGG 1 cut(s) 741
Bst4CI ACNGT 7 cut(s) 18, 36, 68, 244, 295, 403, 836
Bst6I CTCTTC 1 cut(s) 330
BstACI GRCGYC 1 cut(s) 819
BstBAI YACGTR 2 cut(s) 197, 801
BstC8I GCNNGC 1 cut(s) 676
BstDEI CTNAG 6 cut(s) 90, 350, 490, 629, 670, 702
BstDSI CCRYGG 1 cut(s) 789
BstF5I GGATG 4 cut(s) 139, 535, 589, 868
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 4 cut(s) 620, 653, 709, 770
BstMAI GTCTC 2 cut(s) 7, 632
BstMBI GATC 4 cut(s) 617, 650, 706, 767
BstMWI GCNNNNNNNGC 3 cut(s) 124, 169, 390
BstNI CCWGG 1 cut(s) 741
BstSCI CCNGG 1 cut(s) 739
BstSFI CTRYAG 1 cut(s) 189
BstSLI GKGCMC 1 cut(s) 129
BstSNI TACGTA 1 cut(s) 197
BstV1I GCAGC 5 cut(s) 9, 285, 560, 631, 646
BstX2I RGATCY 1 cut(s) 706
BstYI RGATCY 1 cut(s) 706
BstZ17I GTATAC 1 cut(s) 826
Bsu36I CCTNAGG 1 cut(s) 90
BsuRI GGCC 2 cut(s) 57, 893
BtgI CCRYGG 1 cut(s) 789
BtgZI GCGATG 1 cut(s) 882
BtsCI GGATG 4 cut(s) 139, 535, 589, 868
BtsIMutI CAGTG 1 cut(s) 602
Cac8I GCNNGC 1 cut(s) 676
CaiI CAGNNNCTG 2 cut(s) 424, 647
CfoI GCGC 1 cut(s) 165
Cfr13I GGNCC 2 cut(s) 229, 892
CseI GACGC 1 cut(s) 808
Csp6I GTAC 4 cut(s) 95, 194, 198, 715
CviAII CATG 5 cut(s) 540, 594, 718, 790, 842
CviQI GTAC 4 cut(s) 95, 194, 198, 715
DdeI CTNAG 6 cut(s) 90, 350, 490, 629, 670, 702
DpnI GATC 4 cut(s) 619, 652, 708, 769
DpnII GATC 4 cut(s) 617, 650, 706, 767
EaeI YGGCCR 1 cut(s) 55
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
EciI GGCGGA 1 cut(s) 693
Eco105I TACGTA 1 cut(s) 197
Eco130I CCWWGG 2 cut(s) 789, 888
Eco24I GRGCYC 1 cut(s) 732
Eco31I GGTCTC 1 cut(s) 632
Eco32I GATATC 2 cut(s) 474, 882
Eco47I GGWCC 1 cut(s) 229
Eco57I CTGAAG 1 cut(s) 354
Eco81I CCTNAGG 1 cut(s) 90
Eco88I CYCGRG 1 cut(s) 437
EcoO109I RGGNCCY 1 cut(s) 892
EcoRI GAATTC 1 cut(s) 313
EcoRII CCWGG 1 cut(s) 739
EcoRV GATATC 2 cut(s) 474, 882
EcoT14I CCWWGG 2 cut(s) 789, 888
EcoT38I GRGCYC 1 cut(s) 732
ErhI CCWWGG 2 cut(s) 789, 888
FaeI CATG 5 cut(s) 543, 597, 721, 793, 845
FatI CATG 5 cut(s) 539, 593, 717, 789, 841
FauNDI CATATG 1 cut(s) 479
FblI GTMKAC 1 cut(s) 825
Fnu4HI GCNGC 5 cut(s) 23, 274, 549, 645, 660
FokI GGATG 4 cut(s) 146, 522, 596, 875
FriOI GRGCYC 1 cut(s) 732
Fsp4HI GCNGC 5 cut(s) 23, 274, 549, 645, 660
FspBI CTAG 1 cut(s) 305
GlaI GCGC 1 cut(s) 164
GluI GCNGC 5 cut(s) 23, 274, 549, 645, 660
GsaI CCCAGC 1 cut(s) 214
GsuI CTGGAG 1 cut(s) 695
HaeIII GGCC 2 cut(s) 57, 893
HapII CCGG 1 cut(s) 545
HgaI GACGC 1 cut(s) 808
HhaI GCGC 1 cut(s) 165
Hin1I GRCGYC 1 cut(s) 819
Hin1II CATG 5 cut(s) 543, 597, 721, 793, 845
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HincII GTYRAC 1 cut(s) 406
HindII GTYRAC 1 cut(s) 406
HindIII AAGCTT 1 cut(s) 754
HinfI GANTC 2 cut(s) 499, 561
HpaII CCGG 1 cut(s) 545
HphI GGTGA 4 cut(s) 73, 263, 729, 911
Hpy166II GTNNAC 6 cut(s) 46, 127, 229, 406, 487, 826
Hpy188I TCNGA 6 cut(s) 64, 153, 319, 493, 632, 687
Hpy188III TCNNGA 5 cut(s) 352, 425, 558, 615, 704
Hpy8I GTNNAC 6 cut(s) 46, 127, 229, 406, 487, 826
Hpy99I CGWCG 2 cut(s) 44, 824
HpyAV CCTTC 2 cut(s) 465, 898
HpyCH4III ACNGT 7 cut(s) 18, 36, 68, 244, 295, 403, 836
HpyCH4IV ACGT 4 cut(s) 42, 196, 800, 947
HpyCH4V TGCA 7 cut(s) 127, 137, 398, 533, 610, 662, 763
HpyF10VI GCNNNNNNNGC 3 cut(s) 124, 169, 390
HpyF3I CTNAG 6 cut(s) 90, 350, 490, 629, 670, 702
HpySE526I ACGT 4 cut(s) 42, 196, 800, 947
Hsp92I GRCGYC 1 cut(s) 819
Hsp92II CATG 5 cut(s) 543, 597, 721, 793, 845
HspAI GCGC 1 cut(s) 163
Kzo9I GATC 4 cut(s) 617, 650, 706, 767
Lsp1109I GCAGC 5 cut(s) 9, 285, 560, 631, 646
LweI GCATC 4 cut(s) 87, 124, 355, 678
MaeI CTAG 1 cut(s) 305
MaeII ACGT 4 cut(s) 42, 196, 800, 947
MaeIII GTNAC 1 cut(s) 238
MalI GATC 4 cut(s) 619, 652, 708, 769
MboI GATC 4 cut(s) 617, 650, 706, 767
MboII GAAGA 1 cut(s) 347
MflI RGATCY 1 cut(s) 706
MhlI GDGCHC 2 cut(s) 129, 732
MluCI AATT 4 cut(s) 313, 461, 528, 573
MmeI TCCRAC 1 cut(s) 527
MnlI CCTC 8 cut(s) 3, 99, 331, 433, 446, 760, 808, 927
MroXI GAANNNNTTC 2 cut(s) 420, 498
MseI TTAA 5 cut(s) 29, 516, 527, 576, 758
MslI CAYNNNNRTG 2 cut(s) 132, 538
MspA1I CMGCKG 2 cut(s) 644, 659
MspI CCGG 1 cut(s) 545
MspR9I CCNGG 1 cut(s) 741
Mva1269I GAATGC 1 cut(s) 610
MvaI CCWGG 1 cut(s) 741
MwoI GCNNNNNNNGC 3 cut(s) 124, 169, 390
NcoI CCATGG 1 cut(s) 789
NdeI CATATG 1 cut(s) 479
NdeII GATC 4 cut(s) 617, 650, 706, 767
NlaIII CATG 5 cut(s) 543, 597, 721, 793, 845
NlaIV GGNNCC 2 cut(s) 297, 894
PaeR7I CTCGAG 1 cut(s) 437
PctI GAATGC 1 cut(s) 610
PdmI GAANNNNTTC 2 cut(s) 420, 498
PfeI GAWTC 2 cut(s) 499, 561
PflMI CCANNNNNTGG 1 cut(s) 718
PkrI GCNGC 5 cut(s) 24, 275, 550, 646, 661
Ppu21I YACGTR 2 cut(s) 197, 801
Psp6I CCWGG 1 cut(s) 739
PspFI CCCAGC 1 cut(s) 210
PspGI CCWGG 1 cut(s) 739
PspN4I GGNNCC 2 cut(s) 297, 894
PspPI GGNCC 2 cut(s) 229, 892
PspXI VCTCGAGB 1 cut(s) 437
PstNI CAGNNNCTG 2 cut(s) 424, 647
PsuI RGATCY 1 cut(s) 706
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 4 cut(s) 96, 195, 199, 716
RsaNI GTAC 4 cut(s) 95, 194, 198, 715
RseI CAYNNNNRTG 2 cut(s) 132, 538
SaqAI TTAA 5 cut(s) 29, 516, 527, 576, 758
SatI GCNGC 5 cut(s) 23, 274, 549, 645, 660
Sau3AI GATC 4 cut(s) 617, 650, 706, 767
Sau96I GGNCC 2 cut(s) 229, 892
ScrFI CCNGG 1 cut(s) 741
SduI GDGCHC 2 cut(s) 129, 732
SfaNI GCATC 4 cut(s) 87, 124, 355, 678
SfcI CTRYAG 1 cut(s) 189
Sfr274I CTCGAG 1 cut(s) 437
SinI GGWCC 1 cut(s) 229
SlaI CTCGAG 1 cut(s) 437
SmiMI CAYNNNNRTG 2 cut(s) 132, 538
SmlI CTYRAG 2 cut(s) 437, 751
SmoI CTYRAG 2 cut(s) 437, 751
SnaBI TACGTA 1 cut(s) 197
Sse9I AATT 4 cut(s) 313, 461, 528, 573
SsiI CCGC 3 cut(s) 393, 657, 678
SspMI CTAG 1 cut(s) 305
StyD4I CCNGG 1 cut(s) 739
StyI CCWWGG 2 cut(s) 789, 888
TaaI ACNGT 7 cut(s) 18, 36, 68, 244, 295, 403, 836
TaiI ACGT 4 cut(s) 45, 199, 803, 950
TaqI TCGA 4 cut(s) 39, 438, 445, 616
TasI AATT 4 cut(s) 313, 461, 528, 573
TatI WGTACW 1 cut(s) 714
TfiI GAWTC 2 cut(s) 499, 561
Tru1I TTAA 5 cut(s) 29, 516, 527, 576, 758
Tru9I TTAA 5 cut(s) 29, 516, 527, 576, 758
TscAI CASTG 1 cut(s) 609
TseI GCWGC 5 cut(s) 22, 273, 548, 644, 659
TspDTI ATGAA 1 cut(s) 271
TspGWI ACGGA 2 cut(s) 209, 780
TspRI CASTG 1 cut(s) 609
Van91I CCANNNNNTGG 1 cut(s) 718
VneI GTGCAC 1 cut(s) 125
VpaK11BI GGWCC 1 cut(s) 229
XapI RAATTY 1 cut(s) 313
XhoI CTCGAG 1 cut(s) 437
XmiI GTMKAC 1 cut(s) 825
XmnI GAANNNNTTC 2 cut(s) 420, 498
XspI CTAG 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.