Prupe.4G156300_v2.0.a1

NADH kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0007259
Physical Location & Seq
Forward (+)
8957741 .. 8960494
2754 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G156300.4

Sequence Viewer

Length: 1008 bp
ATGTCGAGGAAGAGATTGTTGATGCTGTTAAAACCGATCGACGTTTACTCAGATTCTCAATCAGACGGTCCATCTCGCATCACCAACCCTCACTCTCAGGAATTGCAGATGTTACATCATTTGGAAAATAGGCTAAAGGTGCACAATGATGCAATAAATTTTTGTCAGAATATTTTGTGCCGCAAACCTGTTGAGTGGAAATCTATAGTCCTTAACAATCTATCACAGCCCATCCATGATGTGGATCTTGTAGTTACAGTTGGTGGCGATGGCACTCTATTACAAGCTAGCCATTTCATTGATGACTCAGTTCCAGTTCTAGGAGTGAATTCTGACCCCACACGGGCTGAAGAGGTGGAGAAGCTCAGCAATGAGATCGATGCTACTAGAAGCACAGGCTATCTTTGCGCTGTAACTGTCAATAACTTTGAACAGGTACTGGACAACATCCTCGAGGGTCGAACAATTCCTTCAAAATTGACAAGGATATCCATATGTGTGAACTCGCAAGTGCTTTCAACTTATGCTCTTAATGATGTTTTGATCGCACATCCATGTCCAGCAGCAGTTTCACAGTTCTCATTCAAAATTAAAAGTGATGACCAGCCATGTTCCCCCTTGGTGAACTCTCGATCAAGTGGTCTCAGAGTGTCAACCGCCGCGGGATCAACAGCTGCGATGCTCTCAGCAGGCGGTTTTCTGATGCCCATCTTATCTGAGGATCTCCAGTTTATGGTAAGAGAGCCCATTTCACCAGGAGAGACCCCAAGCTTGATGCACGGATTAATCAAATCTCATCAATCCATGGATGCAACATGGTTTTGTAAAGAGGGTGCCATTTACATTGATGGTTCTCATGTTTTCTATTCTATACAAAATGGGGATACAATTGAGATATCTTCAAGGGCCCCAATTTTGAAGGTTTTCTTATCTCAACGCTTACAGACAACAGTGAAGAAACTTGAGGAGGAATATTTACAGAAAAAAGAAATACTTTGTAAATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

336

Amino Acids

37.1

Weight (kDa)

5.93

Isoelectric Point (pI)

48.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 452
AccB1I GGYRCC 1 cut(s) 833
AccB7I CCANNNNNTGG 2 cut(s) 241, 733
AccII CGCG 1 cut(s) 662
AciI CCGC 5 cut(s) 181, 657, 660, 662, 693
AclWI GGATC 3 cut(s) 252, 673, 729
AcsI RAATTY 2 cut(s) 157, 328
AcuI CTGAAG 1 cut(s) 369
AfaI GTAC 1 cut(s) 438
AfiI CCNNNNNNNGG 4 cut(s) 241, 320, 343, 733
AgsI TTSAA 6 cut(s) 431, 474, 519, 586, 903, 919
AjnI CCWGG 1 cut(s) 754
AluBI AGCT 4 cut(s) 287, 364, 674, 771
AluI AGCT 4 cut(s) 287, 364, 674, 771
Alw21I GWGCWC 1 cut(s) 144
Alw26I GTCTC 2 cut(s) 647, 755
Alw44I GTGCAC 1 cut(s) 140
AlwI GGATC 3 cut(s) 252, 673, 729
AlwNI CAGNNNCTG 1 cut(s) 439
Ama87I CYCGRG 1 cut(s) 452
AoxI GGCC 1 cut(s) 906
ApaI GGGCCC 1 cut(s) 910
ApaLI GTGCAC 1 cut(s) 140
ApeKI GCWGC 2 cut(s) 563, 674
ApoI RAATTY 2 cut(s) 157, 328
AseI ATTAAT 1 cut(s) 785
Asp700I GAANNNNTTC 1 cut(s) 923
AspLEI GCGC 1 cut(s) 410
AspS9I GGNCC 3 cut(s) 68, 906, 907
AsuHPI GGTGA 3 cut(s) 73, 634, 744
AsuNHI GCTAGC 1 cut(s) 287
AvaI CYCGRG 1 cut(s) 452
AvaII GGWCC 1 cut(s) 68
BaeGI GKGCMC 2 cut(s) 144, 910
BanI GGYRCC 1 cut(s) 833
BanII GRGCYC 2 cut(s) 747, 910
Bbv12I GWGCWC 1 cut(s) 144
BbvI GCAGC 2 cut(s) 575, 661
BccI CCATC 5 cut(s) 79, 239, 263, 716, 842
BcgI CGANNNNNNTGC 2 cut(s) 358, 392
BciT130I CCWGG 1 cut(s) 756
BciVI GTATCC 1 cut(s) 877
BcoDI GTCTC 2 cut(s) 647, 755
BfaI CTAG 4 cut(s) 288, 320, 387, 1006
BfmI CTRYAG 1 cut(s) 204
BfuI GTATCC 1 cut(s) 877
BisI GCNGC 4 cut(s) 181, 564, 660, 675
BlpI GCTNAGC 1 cut(s) 365
BlsI GCNGC 4 cut(s) 182, 565, 661, 676
Bme1390I CCNGG 1 cut(s) 756
Bme18I GGWCC 1 cut(s) 68
BmeT110I CYCGRG 1 cut(s) 452
BmgT120I GGNCC 3 cut(s) 68, 906, 907
BmiI GGNNCC 3 cut(s) 835, 908, 909
BmrFI CCNGG 1 cut(s) 756
BmsI GCATC 8 cut(s) 12, 87, 139, 370, 669, 693, 765, 799
BmtI GCTAGC 1 cut(s) 291
BpmI CTGGAG 1 cut(s) 710
Bpu1102I GCTNAGC 1 cut(s) 365
BpuEI CTTGAG 1 cut(s) 983
Bsa29I ATCGAT 1 cut(s) 378
BsaBI GATNNNNATC 2 cut(s) 243, 707
BsaI GGTCTC 2 cut(s) 647, 755
BsaJI CCNNGG 3 cut(s) 618, 660, 804
Bsc4I CCNNNNNNNGG 4 cut(s) 241, 320, 343, 733
Bse1I ACTGG 3 cut(s) 314, 444, 727
Bse3DI GCAATG 1 cut(s) 376
Bse8I GATNNNNATC 2 cut(s) 243, 707
BseBI CCWGG 1 cut(s) 756
BseCI ATCGAT 1 cut(s) 378
BseDI CCNNGG 3 cut(s) 618, 660, 804
BseGI GGATG 4 cut(s) 231, 447, 550, 814
BseJI GATNNNNATC 2 cut(s) 243, 707
BseLI CCNNNNNNNGG 4 cut(s) 241, 320, 343, 733
BseMI GCAATG 1 cut(s) 376
BseMII CTCAG 7 cut(s) 63, 110, 321, 379, 658, 699, 708
BseNI ACTGG 3 cut(s) 314, 444, 727
BseRI GAGGAG 1 cut(s) 980
BseSI GKGCMC 2 cut(s) 144, 910
BseXI GCAGC 2 cut(s) 575, 661
Bsh1236I CGCG 1 cut(s) 662
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 1 cut(s) 908
BshNI GGYRCC 1 cut(s) 833
BshVI ATCGAT 1 cut(s) 378
BsiEI CGRYCG 1 cut(s) 39
BsiHKAI GWGCWC 1 cut(s) 144
BsiHKCI CYCGRG 1 cut(s) 452
BslI CCNNNNNNNGG 4 cut(s) 241, 320, 343, 733
BsmAI GTCTC 2 cut(s) 647, 755
BsnI GGCC 1 cut(s) 908
Bso31I GGTCTC 2 cut(s) 647, 755
BsoBI CYCGRG 1 cut(s) 452
Bsp120I GGGCCC 1 cut(s) 906
Bsp1286I GDGCHC 3 cut(s) 144, 747, 910
Bsp143I GATC 7 cut(s) 36, 244, 375, 543, 632, 665, 721
Bsp1720I GCTNAGC 1 cut(s) 365
Bsp19I CCATGG 1 cut(s) 804
BspACI CCGC 5 cut(s) 181, 657, 660, 662, 693
BspANI GGCC 1 cut(s) 908
BspCNI CTCAG 7 cut(s) 62, 109, 320, 378, 657, 698, 709
BspDI ATCGAT 1 cut(s) 378
BspFNI CGCG 1 cut(s) 662
BspLI GGNNCC 3 cut(s) 835, 908, 909
BspOI GCTAGC 1 cut(s) 291
BspPI GGATC 3 cut(s) 252, 673, 729
BspT107I GGYRCC 1 cut(s) 833
BspTNI GGTCTC 2 cut(s) 647, 755
BsrDI GCAATG 1 cut(s) 376
BsrI ACTGG 3 cut(s) 314, 444, 727
BssECI CCNNGG 3 cut(s) 618, 660, 804
BssMI GATC 7 cut(s) 36, 244, 375, 543, 632, 665, 721
BssT1I CCWWGG 2 cut(s) 618, 804
Bst2UI CCWGG 1 cut(s) 756
Bst4CI ACNGT 5 cut(s) 68, 259, 418, 576, 952
Bst6I CTCTTC 2 cut(s) 5, 345
BstC8I GCNNGC 2 cut(s) 289, 691
BstDEI CTNAG 7 cut(s) 49, 96, 307, 365, 644, 685, 717
BstDSI CCRYGG 2 cut(s) 660, 804
BstF5I GGATG 4 cut(s) 231, 447, 550, 814
BstFNI CGCG 1 cut(s) 662
BstHHI GCGC 1 cut(s) 410
BstKTI GATC 7 cut(s) 39, 247, 378, 546, 635, 668, 724
BstMAI GTCTC 2 cut(s) 647, 755
BstMBI GATC 7 cut(s) 36, 244, 375, 543, 632, 665, 721
BstMCI CGRYCG 1 cut(s) 39
BstMWI GCNNNNNNNGC 2 cut(s) 139, 405
BstNI CCWGG 1 cut(s) 756
BstSCI CCNGG 1 cut(s) 754
BstSFI CTRYAG 1 cut(s) 204
BstSLI GKGCMC 2 cut(s) 144, 910
BstUI CGCG 1 cut(s) 662
BstV1I GCAGC 2 cut(s) 575, 661
BstX2I RGATCY 2 cut(s) 244, 721
BstYI RGATCY 2 cut(s) 244, 721
Bsu15I ATCGAT 1 cut(s) 378
BsuI GTATCC 1 cut(s) 877
BsuRI GGCC 1 cut(s) 908
BsuTUI ATCGAT 1 cut(s) 378
BtgI CCRYGG 2 cut(s) 660, 804
BtgZI GCGATG 2 cut(s) 282, 692
BtsCI GGATG 4 cut(s) 231, 447, 550, 814
BtsIMutI CAGTG 1 cut(s) 957
Cac8I GCNNGC 2 cut(s) 289, 691
CaiI CAGNNNCTG 1 cut(s) 439
CfoI GCGC 1 cut(s) 410
Cfr13I GGNCC 3 cut(s) 68, 906, 907
Cfr42I CCGCGG 1 cut(s) 663
ClaI ATCGAT 1 cut(s) 378
Csp6I GTAC 1 cut(s) 437
CviAII CATG 6 cut(s) 236, 555, 609, 805, 816, 857
CviQI GTAC 1 cut(s) 437
DdeI CTNAG 7 cut(s) 49, 96, 307, 365, 644, 685, 717
DpnI GATC 7 cut(s) 38, 246, 377, 545, 634, 667, 723
DpnII GATC 7 cut(s) 36, 244, 375, 543, 632, 665, 721
Eam1104I CTCTTC 2 cut(s) 5, 345
EarI CTCTTC 2 cut(s) 5, 345
Eco130I CCWWGG 2 cut(s) 618, 804
Eco24I GRGCYC 2 cut(s) 747, 910
Eco31I GGTCTC 2 cut(s) 647, 755
Eco32I GATATC 2 cut(s) 489, 897
Eco47I GGWCC 1 cut(s) 68
Eco57I CTGAAG 1 cut(s) 369
Eco88I CYCGRG 1 cut(s) 452
EcoO109I RGGNCCY 2 cut(s) 906, 907
EcoRI GAATTC 1 cut(s) 328
EcoRII CCWGG 1 cut(s) 754
EcoRV GATATC 2 cut(s) 489, 897
EcoT14I CCWWGG 2 cut(s) 618, 804
EcoT38I GRGCYC 2 cut(s) 747, 910
ErhI CCWWGG 2 cut(s) 618, 804
FaeI CATG 6 cut(s) 239, 558, 612, 808, 819, 860
FalI AAGNNNNNCTT 3 cut(s) 911, 943, 978
FatI CATG 6 cut(s) 235, 554, 608, 804, 815, 856
FauI CCCGC 1 cut(s) 655
FauNDI CATATG 1 cut(s) 494
Fnu4HI GCNGC 4 cut(s) 181, 564, 660, 675
FokI GGATG 4 cut(s) 218, 434, 537, 821
FriOI GRGCYC 2 cut(s) 747, 910
Fsp4HI GCNGC 4 cut(s) 181, 564, 660, 675
FspBI CTAG 4 cut(s) 288, 320, 387, 1006
GlaI GCGC 1 cut(s) 409
GluI GCNGC 4 cut(s) 181, 564, 660, 675
GsuI CTGGAG 1 cut(s) 710
HaeIII GGCC 1 cut(s) 908
HhaI GCGC 1 cut(s) 410
Hin1II CATG 6 cut(s) 239, 558, 612, 808, 819, 860
Hin6I GCGC 1 cut(s) 408
HinP1I GCGC 1 cut(s) 408
HincII GTYRAC 1 cut(s) 654
HindII GTYRAC 1 cut(s) 654
HindIII AAGCTT 1 cut(s) 769
HinfI GANTC 2 cut(s) 53, 305
HphI GGTGA 3 cut(s) 73, 634, 744
Hpy166II GTNNAC 5 cut(s) 46, 142, 502, 625, 654
Hpy188I TCNGA 7 cut(s) 52, 64, 168, 334, 647, 702, 718
Hpy188III TCNNGA 2 cut(s) 98, 630
Hpy8I GTNNAC 5 cut(s) 46, 142, 502, 625, 654
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 2 cut(s) 480, 913
HpyCH4III ACNGT 5 cut(s) 68, 259, 418, 576, 952
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 5 cut(s) 106, 142, 152, 778, 812
HpyF10VI GCNNNNNNNGC 2 cut(s) 139, 405
HpyF3I CTNAG 7 cut(s) 49, 96, 307, 365, 644, 685, 717
HpySE526I ACGT 1 cut(s) 42
Hsp92II CATG 6 cut(s) 239, 558, 612, 808, 819, 860
HspAI GCGC 1 cut(s) 408
KspI CCGCGG 1 cut(s) 663
Kzo9I GATC 7 cut(s) 36, 244, 375, 543, 632, 665, 721
Lsp1109I GCAGC 2 cut(s) 575, 661
LweI GCATC 8 cut(s) 12, 87, 139, 370, 669, 693, 765, 799
MaeI CTAG 4 cut(s) 288, 320, 387, 1006
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 3 cut(s) 111, 253, 412
MalI GATC 7 cut(s) 38, 246, 377, 545, 634, 667, 723
MboI GATC 7 cut(s) 36, 244, 375, 543, 632, 665, 721
MboII GAAGA 4 cut(s) 22, 362, 891, 967
MfeI CAATTG 1 cut(s) 888
MflI RGATCY 2 cut(s) 244, 721
MhlI GDGCHC 3 cut(s) 144, 747, 910
MluCI AATT 8 cut(s) 101, 157, 328, 465, 476, 588, 888, 912
MlyI GAGTC 1 cut(s) 299
MnlI CCTC 8 cut(s) 99, 346, 448, 461, 712, 823, 958, 961
MroXI GAANNNNTTC 1 cut(s) 923
MseI TTAA 5 cut(s) 29, 213, 531, 591, 785
MslI CAYNNNNRTG 3 cut(s) 147, 497, 553
MspA1I CMGCKG 2 cut(s) 662, 674
MspR9I CCNGG 1 cut(s) 756
MunI CAATTG 1 cut(s) 888
MvaI CCWGG 1 cut(s) 756
MvnI CGCG 1 cut(s) 662
MwoI GCNNNNNNNGC 2 cut(s) 139, 405
NcoI CCATGG 1 cut(s) 804
NdeI CATATG 1 cut(s) 494
NdeII GATC 7 cut(s) 36, 244, 375, 543, 632, 665, 721
NheI GCTAGC 1 cut(s) 287
NlaIII CATG 6 cut(s) 239, 558, 612, 808, 819, 860
NlaIV GGNNCC 3 cut(s) 835, 908, 909
PaeR7I CTCGAG 1 cut(s) 452
PdmI GAANNNNTTC 1 cut(s) 923
PfeI GAWTC 1 cut(s) 53
PflMI CCANNNNNTGG 2 cut(s) 241, 733
PkrI GCNGC 4 cut(s) 182, 565, 661, 676
Ple19I CGATCG 1 cut(s) 39
PleI GAGTC 1 cut(s) 299
PpsI GAGTC 1 cut(s) 299
PshBI ATTAAT 1 cut(s) 785
Psp6I CCWGG 1 cut(s) 754
PspGI CCWGG 1 cut(s) 754
PspN4I GGNNCC 3 cut(s) 835, 908, 909
PspOMI GGGCCC 1 cut(s) 906
PspPI GGNCC 3 cut(s) 68, 906, 907
PspXI VCTCGAGB 1 cut(s) 452
PstNI CAGNNNCTG 1 cut(s) 439
PsuI RGATCY 2 cut(s) 244, 721
PvuI CGATCG 1 cut(s) 39
PvuII CAGCTG 1 cut(s) 674
RsaI GTAC 1 cut(s) 438
RsaNI GTAC 1 cut(s) 437
RseI CAYNNNNRTG 3 cut(s) 147, 497, 553
SacII CCGCGG 1 cut(s) 663
SaqAI TTAA 5 cut(s) 29, 213, 531, 591, 785
SatI GCNGC 4 cut(s) 181, 564, 660, 675
Sau3AI GATC 7 cut(s) 36, 244, 375, 543, 632, 665, 721
Sau96I GGNCC 3 cut(s) 68, 906, 907
SchI GAGTC 1 cut(s) 299
ScrFI CCNGG 1 cut(s) 756
SduI GDGCHC 3 cut(s) 144, 747, 910
SfaNI GCATC 8 cut(s) 12, 87, 139, 370, 669, 693, 765, 799
SfcI CTRYAG 1 cut(s) 204
Sfr274I CTCGAG 1 cut(s) 452
Sfr303I CCGCGG 1 cut(s) 663
SgrBI CCGCGG 1 cut(s) 663
SinI GGWCC 1 cut(s) 68
SlaI CTCGAG 1 cut(s) 452
SmiMI CAYNNNNRTG 3 cut(s) 147, 497, 553
SmlI CTYRAG 2 cut(s) 452, 962
SmoI CTYRAG 2 cut(s) 452, 962
Sse9I AATT 8 cut(s) 101, 157, 328, 465, 476, 588, 888, 912
SsiI CCGC 5 cut(s) 181, 657, 660, 662, 693
SspI AATATT 2 cut(s) 172, 974
SspMI CTAG 4 cut(s) 288, 320, 387, 1006
StyD4I CCNGG 1 cut(s) 754
StyI CCWWGG 2 cut(s) 618, 804
TaaI ACNGT 5 cut(s) 68, 259, 418, 576, 952
TaiI ACGT 1 cut(s) 45
TaqI TCGA 6 cut(s) 5, 39, 378, 453, 460, 631
TasI AATT 8 cut(s) 101, 157, 328, 465, 476, 588, 888, 912
TauI GCSGC 2 cut(s) 183, 662
TfiI GAWTC 1 cut(s) 53
Tru1I TTAA 5 cut(s) 29, 213, 531, 591, 785
Tru9I TTAA 5 cut(s) 29, 213, 531, 591, 785
TscAI CASTG 1 cut(s) 957
TseI GCWGC 2 cut(s) 563, 674
TspDTI ATGAA 1 cut(s) 286
TspGWI ACGGA 1 cut(s) 795
TspRI CASTG 1 cut(s) 957
Van91I CCANNNNNTGG 2 cut(s) 241, 733
VneI GTGCAC 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 68
VspI ATTAAT 1 cut(s) 785
XapI RAATTY 2 cut(s) 157, 328
XcmI CCANNNNNNNNNTGG 1 cut(s) 238
XhoI CTCGAG 1 cut(s) 452
XmnI GAANNNNTTC 1 cut(s) 923
XspI CTAG 4 cut(s) 288, 320, 387, 1006
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.