MD03G1248900.v1.1

NADH kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007259
Physical Location & Seq
Reverse (-)
33636188 .. 33637647
1460 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1248900.v1.1.491

Sequence Viewer

Length: 627 bp
ATGGCACTCTATTACAACTTACAAGGTAGCCATTTCATTGATGACTCGGTTCCAGTTCTAGGAGTGAATTCTGACCCCACACAAGCTGAAGAGGTACTGGCCAACATCCTCGAGGGTCGAGCTAATCCTTCCAAATTGACAAGGATATTAATACGTGTAAACTCAAAAGTGCTTCCAACTTATGCTCTCAATGATATTTTAATTTCACATCCATGTCCAGCAGCACTTTCACGATTGTCATTCAAAATTAAAAAGGATGACCAGCCATGGTCCCTGCTGGTCCATTCTCGATCAAGTGGTCTCAGAGTTTCAACTGCTGCTGGATCAACCACTGCAATGCTCTCAGCAGGCGGATTTCCAATGCCCATCTTATCTCAGGATCTCCAGTATATGGTAATAGAGCCCATTTCACCAGGAGACACCTCAAGCTTAATGCACGGATTAATCAAATCTCATCAGTCCAAAGAGGGTGTCACATACATTGACGGTTCTGATGTACAGAGAGAATCAGTGAAGAAACATGAGGAGGAATGGTTAGATAGAAATATTTTTTTTGTAGATACGAGCTGTAAATACCGCGGAACTTCTGTAAACATGCGTTCAGATTGTAGGGTTTTAATTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

209

Amino Acids

22.98

Weight (kDa)

6.59

Isoelectric Point (pI)

55.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 110
AccB7I CCANNNNNTGG 1 cut(s) 391
AccII CGCG 1 cut(s) 579
AciI CCGC 3 cut(s) 351, 577, 579
AclWI GGATC 2 cut(s) 331, 387
AcoI YGGCCR 1 cut(s) 99
AcsI RAATTY 1 cut(s) 67
AcuI CTGAAG 1 cut(s) 108
AfaI GTAC 2 cut(s) 96, 498
AfiI CCNNNNNNNGG 2 cut(s) 59, 391
AflIII ACRYGT 1 cut(s) 154
AgsI TTSAA 2 cut(s) 244, 312
AjnI CCWGG 1 cut(s) 412
AluBI AGCT 4 cut(s) 86, 122, 429, 567
AluI AGCT 4 cut(s) 86, 122, 429, 567
Alw26I GTCTC 2 cut(s) 305, 411
AlwI GGATC 2 cut(s) 331, 387
Ama87I CYCGRG 1 cut(s) 110
AoxI GGCC 1 cut(s) 99
ApeKI GCWGC 2 cut(s) 221, 317
ApoI RAATTY 1 cut(s) 67
ArsI GACNNNNNNTTYG 2 cut(s) 456, 488
AseI ATTAAT 2 cut(s) 149, 443
AspS9I GGNCC 2 cut(s) 270, 280
AsuHPI GGTGA 1 cut(s) 402
AvaI CYCGRG 1 cut(s) 110
AvaII GGWCC 2 cut(s) 270, 280
BalI TGGCCA 1 cut(s) 101
BanII GRGCYC 1 cut(s) 405
BbvI GCAGC 2 cut(s) 233, 304
BccI CCATC 1 cut(s) 374
BciT130I CCWGG 1 cut(s) 414
BcoDI GTCTC 2 cut(s) 305, 411
BfaI CTAG 1 cut(s) 59
BisI GCNGC 2 cut(s) 222, 318
BlsI GCNGC 2 cut(s) 223, 319
Bme1390I CCNGG 1 cut(s) 414
Bme18I GGWCC 2 cut(s) 270, 280
BmeT110I CYCGRG 1 cut(s) 110
BmgT120I GGNCC 2 cut(s) 270, 280
BmiI GGNNCC 2 cut(s) 51, 272
BmrFI CCNGG 1 cut(s) 414
BpmI CTGGAG 1 cut(s) 368
BpuEI CTTGAG 1 cut(s) 409
BsaAI YACGTR 1 cut(s) 155
BsaI GGTCTC 1 cut(s) 305
BsaJI CCNNGG 2 cut(s) 266, 577
BsaXI ACNNNNNCTCC 2 cut(s) 518, 548
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 391
Bse1I ACTGG 3 cut(s) 53, 102, 385
Bse3DI GCAATG 1 cut(s) 342
BseBI CCWGG 1 cut(s) 414
BseDI CCNNGG 2 cut(s) 266, 577
BseGI GGATG 3 cut(s) 105, 208, 262
BseLI CCNNNNNNNGG 2 cut(s) 59, 391
BseMI GCAATG 1 cut(s) 342
BseMII CTCAG 3 cut(s) 316, 357, 389
BseNI ACTGG 3 cut(s) 53, 102, 385
BseRI GAGGAG 1 cut(s) 539
BseXI GCAGC 2 cut(s) 233, 304
Bsh1236I CGCG 1 cut(s) 579
BshFI GGCC 1 cut(s) 101
BsiHKCI CYCGRG 1 cut(s) 110
BslFI GGGAC 1 cut(s) 256
BslI CCNNNNNNNGG 2 cut(s) 59, 391
BsmAI GTCTC 2 cut(s) 305, 411
BsmFI GGGAC 1 cut(s) 256
BsnI GGCC 1 cut(s) 101
Bso31I GGTCTC 1 cut(s) 305
BsoBI CYCGRG 1 cut(s) 110
Bsp1286I GDGCHC 1 cut(s) 405
Bsp1407I TGTACA 1 cut(s) 496
Bsp143I GATC 3 cut(s) 290, 323, 379
Bsp19I CCATGG 1 cut(s) 266
BspACI CCGC 3 cut(s) 351, 577, 579
BspANI GGCC 1 cut(s) 101
BspCNI CTCAG 3 cut(s) 315, 356, 388
BspFNI CGCG 1 cut(s) 579
BspLI GGNNCC 2 cut(s) 51, 272
BspPI GGATC 2 cut(s) 331, 387
BspTNI GGTCTC 1 cut(s) 305
BsrDI GCAATG 1 cut(s) 342
BsrGI TGTACA 1 cut(s) 496
BsrI ACTGG 3 cut(s) 53, 102, 385
BssECI CCNNGG 2 cut(s) 266, 577
BssMI GATC 3 cut(s) 290, 323, 379
BssT1I CCWWGG 1 cut(s) 266
Bst2UI CCWGG 1 cut(s) 414
Bst4CI ACNGT 1 cut(s) 488
Bst6I CTCTTC 1 cut(s) 84
BstAUI TGTACA 1 cut(s) 496
BstBAI YACGTR 1 cut(s) 155
BstC8I GCNNGC 1 cut(s) 349
BstDEI CTNAG 3 cut(s) 302, 343, 375
BstDSI CCRYGG 2 cut(s) 266, 577
BstF5I GGATG 3 cut(s) 105, 208, 262
BstFNI CGCG 1 cut(s) 579
BstKTI GATC 3 cut(s) 293, 326, 382
BstMAI GTCTC 2 cut(s) 305, 411
BstMBI GATC 3 cut(s) 290, 323, 379
BstNI CCWGG 1 cut(s) 414
BstNSI RCATGY 1 cut(s) 598
BstSCI CCNGG 1 cut(s) 412
BstUI CGCG 1 cut(s) 579
BstV1I GCAGC 2 cut(s) 233, 304
BstX2I RGATCY 1 cut(s) 379
BstYI RGATCY 1 cut(s) 379
BsuRI GGCC 1 cut(s) 101
BtgI CCRYGG 2 cut(s) 266, 577
BtsCI GGATG 3 cut(s) 105, 208, 262
BtsI GCAGTG 1 cut(s) 330
BtsIMutI CAGTG 2 cut(s) 330, 516
Cac8I GCNNGC 1 cut(s) 349
Cfr13I GGNCC 2 cut(s) 270, 280
Cfr42I CCGCGG 1 cut(s) 580
Csp6I GTAC 2 cut(s) 95, 497
CviAII CATG 4 cut(s) 213, 267, 521, 595
CviJI RGCY 8 cut(s) 30, 86, 101, 122, 265, 403, 429, 567
CviKI_1 RGCY 8 cut(s) 30, 86, 101, 122, 265, 403, 429, 567
CviQI GTAC 2 cut(s) 95, 497
DdeI CTNAG 3 cut(s) 302, 343, 375
DpnI GATC 3 cut(s) 292, 325, 381
DpnII GATC 3 cut(s) 290, 323, 379
EaeI YGGCCR 1 cut(s) 99
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
EciI GGCGGA 1 cut(s) 366
Eco130I CCWWGG 1 cut(s) 266
Eco24I GRGCYC 1 cut(s) 405
Eco31I GGTCTC 1 cut(s) 305
Eco47I GGWCC 2 cut(s) 270, 280
Eco57I CTGAAG 1 cut(s) 108
Eco88I CYCGRG 1 cut(s) 110
EcoRI GAATTC 1 cut(s) 67
EcoRII CCWGG 1 cut(s) 412
EcoT14I CCWWGG 1 cut(s) 266
EcoT38I GRGCYC 1 cut(s) 405
ErhI CCWWGG 1 cut(s) 266
FaeI CATG 4 cut(s) 216, 270, 524, 598
FaiI YATR 8 cut(s) 183, 214, 268, 390, 392, 478, 522, 596
FaqI GGGAC 1 cut(s) 256
FatI CATG 4 cut(s) 212, 266, 520, 594
Fnu4HI GCNGC 2 cut(s) 222, 318
FokI GGATG 3 cut(s) 92, 195, 269
FriOI GRGCYC 1 cut(s) 405
Fsp4HI GCNGC 2 cut(s) 222, 318
FspBI CTAG 1 cut(s) 59
GluI GCNGC 2 cut(s) 222, 318
GsuI CTGGAG 1 cut(s) 368
HaeIII GGCC 1 cut(s) 101
Hin1II CATG 4 cut(s) 216, 270, 524, 598
HindIII AAGCTT 1 cut(s) 427
HinfI GANTC 2 cut(s) 44, 506
HphI GGTGA 1 cut(s) 402
Hpy166II GTNNAC 2 cut(s) 160, 592
Hpy188I TCNGA 4 cut(s) 73, 305, 493, 604
Hpy188III TCNNGA 3 cut(s) 231, 288, 377
Hpy8I GTNNAC 2 cut(s) 160, 592
HpyAV CCTTC 1 cut(s) 138
HpyCH4III ACNGT 1 cut(s) 488
HpyCH4IV ACGT 1 cut(s) 154
HpyCH4V TGCA 2 cut(s) 335, 436
HpyF3I CTNAG 3 cut(s) 302, 343, 375
HpySE526I ACGT 1 cut(s) 154
Hsp92II CATG 4 cut(s) 216, 270, 524, 598
KspI CCGCGG 1 cut(s) 580
Kzo9I GATC 3 cut(s) 290, 323, 379
Lsp1109I GCAGC 2 cut(s) 233, 304
MaeI CTAG 1 cut(s) 59
MaeII ACGT 1 cut(s) 154
MaeIII GTNAC 1 cut(s) 472
MalI GATC 3 cut(s) 292, 325, 381
MboI GATC 3 cut(s) 290, 323, 379
MboII GAAGA 2 cut(s) 101, 526
MflI RGATCY 1 cut(s) 379
MhlI GDGCHC 1 cut(s) 405
MlsI TGGCCA 1 cut(s) 101
MluCI AATT 5 cut(s) 67, 134, 201, 246, 618
MluNI TGGCCA 1 cut(s) 101
MlyI GAGTC 1 cut(s) 38
MmeI TCCRAC 1 cut(s) 200
MnlI CCTC 7 cut(s) 85, 106, 119, 433, 460, 517, 520
Mox20I TGGCCA 1 cut(s) 101
MscI TGGCCA 1 cut(s) 101
MseI TTAA 6 cut(s) 149, 200, 249, 431, 443, 617
MslI CAYNNNNRTG 2 cut(s) 211, 335
Msp20I TGGCCA 1 cut(s) 101
MspA1I CMGCKG 1 cut(s) 579
MspR9I CCNGG 1 cut(s) 414
MvaI CCWGG 1 cut(s) 414
MvnI CGCG 1 cut(s) 579
NcoI CCATGG 1 cut(s) 266
NdeII GATC 3 cut(s) 290, 323, 379
NlaIII CATG 4 cut(s) 216, 270, 524, 598
NlaIV GGNNCC 2 cut(s) 51, 272
NmuCI GTSAC 1 cut(s) 472
NspI RCATGY 1 cut(s) 598
PaeR7I CTCGAG 1 cut(s) 110
PfeI GAWTC 1 cut(s) 506
PflMI CCANNNNNTGG 1 cut(s) 391
PkrI GCNGC 2 cut(s) 223, 319
PleI GAGTC 1 cut(s) 38
PpsI GAGTC 1 cut(s) 38
Ppu21I YACGTR 1 cut(s) 155
PshBI ATTAAT 2 cut(s) 149, 443
Psp6I CCWGG 1 cut(s) 412
PspGI CCWGG 1 cut(s) 412
PspN4I GGNNCC 2 cut(s) 51, 272
PspPI GGNCC 2 cut(s) 270, 280
PspXI VCTCGAGB 1 cut(s) 110
PsuI RGATCY 1 cut(s) 379
RsaI GTAC 2 cut(s) 96, 498
RsaNI GTAC 2 cut(s) 95, 497
RseI CAYNNNNRTG 2 cut(s) 211, 335
SacII CCGCGG 1 cut(s) 580
SaqAI TTAA 6 cut(s) 149, 200, 249, 431, 443, 617
SatI GCNGC 2 cut(s) 222, 318
Sau3AI GATC 3 cut(s) 290, 323, 379
Sau96I GGNCC 2 cut(s) 270, 280
SchI GAGTC 1 cut(s) 38
ScrFI CCNGG 1 cut(s) 414
SduI GDGCHC 1 cut(s) 405
SetI ASST 8 cut(s) 28, 88, 96, 124, 157, 425, 431, 569
Sfr274I CTCGAG 1 cut(s) 110
Sfr303I CCGCGG 1 cut(s) 580
SgrBI CCGCGG 1 cut(s) 580
SinI GGWCC 2 cut(s) 270, 280
SlaI CTCGAG 1 cut(s) 110
SmiMI CAYNNNNRTG 2 cut(s) 211, 335
SmlI CTYRAG 2 cut(s) 110, 424
SmoI CTYRAG 2 cut(s) 110, 424
Sse9I AATT 5 cut(s) 67, 134, 201, 246, 618
SsiI CCGC 3 cut(s) 351, 577, 579
SspI AATATT 1 cut(s) 547
SspMI CTAG 1 cut(s) 59
StyD4I CCNGG 1 cut(s) 412
StyI CCWWGG 1 cut(s) 266
TaaI ACNGT 1 cut(s) 488
TaiI ACGT 1 cut(s) 157
TaqI TCGA 3 cut(s) 111, 118, 289
TasI AATT 5 cut(s) 67, 134, 201, 246, 618
TatI WGTACW 1 cut(s) 496
TfiI GAWTC 1 cut(s) 506
Tru1I TTAA 6 cut(s) 149, 200, 249, 431, 443, 617
Tru9I TTAA 6 cut(s) 149, 200, 249, 431, 443, 617
TscAI CASTG 2 cut(s) 337, 516
TseFI GTSAC 1 cut(s) 472
TseI GCWGC 2 cut(s) 221, 317
Tsp45I GTSAC 1 cut(s) 472
TspDTI ATGAA 1 cut(s) 25
TspGWI ACGGA 1 cut(s) 453
TspRI CASTG 2 cut(s) 337, 516
Van91I CCANNNNNTGG 1 cut(s) 391
VpaK11BI GGWCC 2 cut(s) 270, 280
VspI ATTAAT 2 cut(s) 149, 443
XapI RAATTY 1 cut(s) 67
XceI RCATGY 1 cut(s) 598
XhoI CTCGAG 1 cut(s) 110
XspI CTAG 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.