FvH4_3g20020

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003941
Physical Location & Seq
Reverse (-)
13166311 .. 13171498
5188 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g20020.t6

Sequence Viewer

Length: 1884 bp
ATGTTTTCGGAGACTGTGAGCAGTTCTAGGAGCATGGACGAGGACGACGTCGTCGTGATCGACCCTCCGGTTAGTCTGACTCGGAAACCGCGCAAAGGCAAAGAGGCTGTTGTGCATGATGTGATCGAAATTGACGATGATGAAGATTCTGACACTGCAATGTTTATGAATAAGAAGAGATTTCGCAGAAGTTATGAAAAGAAGGCCATAAAATACTCTTCCAATGATTACCTTGATCATCAAGCTAAGGGAGCTGTGGCCAATAATTTTGTTAGTTCTGGTAAGAGTTCTTCCTCAGCATCACATAAGGTTGCCTATGGTGATCTTTACTTGGATGAGGTAATTGATGTTGACGAGTATGCCCAATTGCAATCCCATTTTGATAATGTGGATTTTCCTCCTGGAGTAGAGGCGCCTATGCCACCTATCCCTTGGTTTGATCCACCTAAGAGTACTAAAAGTGCAGTTTCTGAAAGTAGTTCTTTTGATACAAAGTATCAGGCTGCTAACAGCTTTGCCTCAGCATCGCATAACGTGATCGACATTTATGGTCCCAGTTCTGGTGCATCTTATTCCAGTGGTGACTATGCTGGTCTTTATTTGGACGAGTTTATAGATATCGATGAGTATGCCCAAATGACAGCTTATTTAGATGAAGTGGATGTTACTCCTGGAATAGAAGCACATTTCCCAGTGGGTCTCTCGTTATCAGATTTTAGCACGAGGAGTGTAAAACCAGTTTCTGGAAGTAGTTCAAGCTTGAAGAGCCAAAACGATGATGTTAGCCACCCTCTTGGGGTATCATCATCTTGGATACTTCCACAAGTTTCCAGCAGCAAAATGATGTCTCATATGGGATTTAGCTATCTGCAACAACAAGGGAGCGCTTCTAGTCTCTCTTTGGGAGCAGAATCATCAAACTTTCAAACACAAACTCAAAATGATGTCTCCGTGAAGCAAGTTTCTAGCATGAGTTCAGATAAAACTGTGGGGAAGGTAGATGGTTTCAAACAATTTGATACAGTTGTAGATCATTCAGATCATCATTATTCTAAGACAAGTCTTTCAACAAAGCAGAAGAACTGGGCAAAGAAAATTCAGGGAGAGTGGAAGATCCTGGAGAAAGACTTGCCTGATATGATATCTGTAAGAGTTTATGAGACAAGAATGGATCTTCTGAGAGCTGTAATTGTTGGAGCAGAGGGTACTCCTTACCATGATGGTCTCTTTTTCTTTGATGTTTCCTTCCCCAGTGGTTACCCCAAAGGGCCGCCTCATGTCTACTACCACGCTCATGGCCTTCGACTAAACCCAAACCTGTATGCTTGTGGAAAAGTATGCCTCAGCCTTCTTAATACTTGGTCTGGGGGCAAAGAAGAAAAGTGGATTCCTAGCAAGTCAACGATGCTTCAGGTTTTGGTCTCAATACAAGGGCTGATCTTGAATTCAGAGCCTTACTTCAATGAGCCTGGATGGGCACCTATGAAGGGTACACCATCTGGTGTAACACAATCAAAAAATTACAATGAGAACACATTCATCCTATCTCTGAGGACGATGGTGTACATGATGAAGACGCCTCCAAAGCATTTTGAGGAGTTTGTTATCGGGCATTTCTGCAATCGTACTCATGACATTCTAGGTGCATGTAAAGCATATATGGATGGAGCTCAAGTGGGGTCTCTAGTCAAAGGGTGTCTCTGCCAAGCTCATGACTCATCAAAATACTGTGATGAGAGGATGCCGGCAATGGTGAACCTTGACCAACTAAGGAGAAGGCTACACTCTTTCAAAAAAAAGAAAAAGAAAAAAGGAGAAGGCTACAGTACTGATGCTCACGACCGGTACAGAACCGCGACATTCTGCTGGCAGGGTAGATCATGA

Protein Analysis

628

Amino Acids

69.73

Weight (kDa)

6.17

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 313 - 432 3.8e-21 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 50
AatII GACGTC 1 cut(s) 51
AccB1I GGYRCC 2 cut(s) 412, 1477
AccB7I CCANNNNNTGG 1 cut(s) 743
AccI GTMKAC 1 cut(s) 1281
AccII CGCG 2 cut(s) 91, 1856
AciI CCGC 3 cut(s) 89, 1271, 1854
AclWI GGATC 3 cut(s) 434, 1108, 1179
AcoI YGGCCR 1 cut(s) 258
AcsI RAATTY 2 cut(s) 1095, 1444
AcuI CTGAAG 1 cut(s) 1394
AcyI GRCGYC 3 cut(s) 48, 413, 1577
AfaI GTAC 7 cut(s) 454, 1207, 1492, 1565, 1627, 1828, 1847
AfeI AGCGCT 1 cut(s) 886
AfiI CCNNNNNNNGG 5 cut(s) 95, 560, 743, 796, 1487
AgeI ACCGGT 1 cut(s) 1842
AgsI TTSAA 8 cut(s) 756, 763, 926, 1009, 1068, 1444, 1462, 1792
AjnI CCWGG 4 cut(s) 400, 670, 1116, 1468
AluBI AGCT 9 cut(s) 245, 254, 513, 644, 759, 864, 1184, 1670, 1709
AluI AGCT 9 cut(s) 245, 254, 513, 644, 759, 864, 1184, 1670, 1709
Alw21I GWGCWC 1 cut(s) 1672
AlwI GGATC 3 cut(s) 434, 1108, 1179
AlwNI CAGNNNCTG 2 cut(s) 470, 743
Aor51HI AGCGCT 1 cut(s) 886
AoxI GGCC 4 cut(s) 204, 258, 1268, 1297
ApeKI GCWGC 2 cut(s) 503, 834
ApoI RAATTY 2 cut(s) 1095, 1444
AsiGI ACCGGT 1 cut(s) 1842
Asp700I GAANNNNTTC 2 cut(s) 751, 1535
AspLEI GCGC 3 cut(s) 93, 415, 887
AspS9I GGNCC 2 cut(s) 551, 1268
AsuHPI GGTGA 3 cut(s) 332, 593, 1765
AvaII GGWCC 1 cut(s) 551
BaeGI GKGCMC 1 cut(s) 1480
BaeI ACNNNNGTAYC 2 cut(s) 1011, 1044
BalI TGGCCA 1 cut(s) 260
BanI GGYRCC 2 cut(s) 412, 1477
BanII GRGCYC 1 cut(s) 1672
BarI GAAGNNNNNNTAC 2 cut(s) 274, 306
BauI CACGAG 1 cut(s) 721
BbsI GAAGAC 1 cut(s) 1580
Bbv12I GWGCWC 1 cut(s) 1672
BbvCI CCTCAGC 3 cut(s) 295, 520, 1343
BbvI GCAGC 2 cut(s) 490, 846
BccI CCATC 6 cut(s) 995, 1214, 1467, 1504, 1552, 1658
BcgI CGANNNNNNTGC 2 cut(s) 611, 645
BciT130I CCWGG 4 cut(s) 402, 672, 1118, 1470
BciVI GTATCC 1 cut(s) 807
BclI TGATCA 1 cut(s) 235
BfaI CTAG 6 cut(s) 27, 891, 966, 1392, 1640, 1685
BfmI CTRYAG 1 cut(s) 1822
BfoI RGCGCY 2 cut(s) 416, 888
BfuI GTATCC 1 cut(s) 807
BisI GCNGC 3 cut(s) 504, 835, 1271
BlsI GCNGC 3 cut(s) 505, 836, 1272
BmcAI AGTACT 2 cut(s) 454, 1828
Bme1390I CCNGG 4 cut(s) 402, 672, 1118, 1470
Bme18I GGWCC 1 cut(s) 551
BmgT120I GGNCC 2 cut(s) 551, 1268
BmiI GGNNCC 3 cut(s) 414, 553, 1479
BmrFI CCNGG 4 cut(s) 402, 672, 1118, 1470
BmrI ACTGGG 4 cut(s) 549, 686, 1093, 1245
BmsI GCATC 6 cut(s) 308, 533, 575, 1395, 1731, 1822
BmuI ACTGGG 4 cut(s) 549, 686, 1093, 1245
BpiI GAAGAC 1 cut(s) 1580
BpmI CTGGAG 2 cut(s) 423, 1139
Bpu10I CCTNAGC 4 cut(s) 246, 295, 520, 1343
BpuEI CTTGAG 1 cut(s) 1656
Bsa29I ATCGAT 1 cut(s) 621
BsaHI GRCGYC 3 cut(s) 48, 413, 1577
BsaI GGTCTC 4 cut(s) 704, 1229, 1426, 1686
BsaJI CCNNGG 1 cut(s) 431
BsaWI WCCGGW 2 cut(s) 67, 1842
BsaXI ACNNNNNCTCC 2 cut(s) 1659, 1689
Bsc4I CCNNNNNNNGG 5 cut(s) 95, 560, 743, 796, 1487
Bse118I RCCGGY 2 cut(s) 1744, 1842
Bse1I ACTGG 6 cut(s) 555, 576, 692, 737, 1088, 1251
Bse3DI GCAATG 2 cut(s) 165, 1755
BseBI CCWGG 4 cut(s) 402, 672, 1118, 1470
BseCI ATCGAT 1 cut(s) 621
BseDI CCNNGG 1 cut(s) 431
BseGI GGATG 6 cut(s) 340, 667, 1478, 1539, 1669, 1746
BseLI CCNNNNNNNGG 5 cut(s) 95, 560, 743, 796, 1487
BseMI GCAATG 2 cut(s) 165, 1755
BseMII CTCAG 5 cut(s) 309, 534, 1169, 1357, 1541
BseNI ACTGG 6 cut(s) 555, 576, 692, 737, 1088, 1251
BseRI GAGGAG 2 cut(s) 739, 1610
BseSI GKGCMC 1 cut(s) 1480
BseXI GCAGC 2 cut(s) 490, 846
BsgI GTGCAG 1 cut(s) 483
Bsh1236I CGCG 2 cut(s) 91, 1856
Bsh1285I CGRYCG 1 cut(s) 1843
BshFI GGCC 4 cut(s) 206, 260, 1270, 1299
BshNI GGYRCC 2 cut(s) 412, 1477
BshTI ACCGGT 1 cut(s) 1842
BshVI ATCGAT 1 cut(s) 621
BsiEI CGRYCG 1 cut(s) 1843
BsiHKAI GWGCWC 1 cut(s) 1672
BsiSI CCGG 3 cut(s) 68, 1745, 1843
BslFI GGGAC 1 cut(s) 537
BslI CCNNNNNNNGG 5 cut(s) 95, 560, 743, 796, 1487
BsmFI GGGAC 1 cut(s) 537
BsnI GGCC 4 cut(s) 206, 260, 1270, 1299
Bso31I GGTCTC 4 cut(s) 704, 1229, 1426, 1686
Bsp1286I GDGCHC 2 cut(s) 1480, 1672
Bsp1407I TGTACA 1 cut(s) 1563
BspACI CCGC 3 cut(s) 89, 1271, 1854
BspANI GGCC 4 cut(s) 206, 260, 1270, 1299
BspCNI CTCAG 5 cut(s) 308, 533, 1170, 1356, 1542
BspDI ATCGAT 1 cut(s) 621
BspFNI CGCG 2 cut(s) 91, 1856
BspHI TCATGA 3 cut(s) 1630, 1711, 1880
BspLI GGNNCC 3 cut(s) 414, 553, 1479
BspPI GGATC 3 cut(s) 434, 1108, 1179
BspQI GCTCTTC 1 cut(s) 758
BspT107I GGYRCC 2 cut(s) 412, 1477
BspTNI GGTCTC 4 cut(s) 704, 1229, 1426, 1686
BsrDI GCAATG 2 cut(s) 165, 1755
BsrFI RCCGGY 2 cut(s) 1744, 1842
BsrGI TGTACA 1 cut(s) 1563
BsrI ACTGG 6 cut(s) 555, 576, 692, 737, 1088, 1251
BssAI RCCGGY 2 cut(s) 1744, 1842
BssECI CCNNGG 1 cut(s) 431
BssNI GRCGYC 3 cut(s) 48, 413, 1577
BssSI CACGAG 1 cut(s) 721
BssT1I CCWWGG 1 cut(s) 431
Bst2BI CACGAG 1 cut(s) 721
Bst2UI CCWGG 4 cut(s) 402, 672, 1118, 1470
Bst4CI ACNGT 5 cut(s) 16, 988, 1024, 1730, 1826
Bst6I CTCTTC 3 cut(s) 170, 223, 758
BstACI GRCGYC 3 cut(s) 48, 413, 1577
BstAUI TGTACA 1 cut(s) 1563
BstC8I GCNNGC 2 cut(s) 1746, 1868
BstDEI CTNAG 9 cut(s) 246, 295, 447, 520, 1053, 1178, 1343, 1550, 1769
BstEII GGTNACC 1 cut(s) 1256
BstF5I GGATG 6 cut(s) 340, 667, 1478, 1539, 1669, 1746
BstFNI CGCG 2 cut(s) 91, 1856
BstH2I RGCGCY 2 cut(s) 416, 888
BstHHI GCGC 3 cut(s) 93, 415, 887
BstMCI CGRYCG 1 cut(s) 1843
BstMWI GCNNNNNNNGC 4 cut(s) 251, 765, 1585, 1652
BstNI CCWGG 4 cut(s) 402, 672, 1118, 1470
BstNSI RCATGY 1 cut(s) 1650
BstPI GGTNACC 1 cut(s) 1256
BstSCI CCNGG 4 cut(s) 400, 670, 1116, 1468
BstSFI CTRYAG 1 cut(s) 1822
BstSLI GKGCMC 1 cut(s) 1480
BstUI CGCG 2 cut(s) 91, 1856
BstV1I GCAGC 2 cut(s) 490, 846
BstV2I GAAGAC 1 cut(s) 1580
BstX2I RGATCY 2 cut(s) 1113, 1171
BstXI CCANNNNNNTGG 2 cut(s) 794, 1295
BstYI RGATCY 2 cut(s) 1113, 1171
Bsu15I ATCGAT 1 cut(s) 621
BsuI GTATCC 1 cut(s) 807
BsuRI GGCC 4 cut(s) 206, 260, 1270, 1299
BsuTUI ATCGAT 1 cut(s) 621
BtgZI GCGATG 1 cut(s) 510
BtsCI GGATG 6 cut(s) 340, 667, 1478, 1539, 1669, 1746
BtsI GCAGTG 1 cut(s) 153
BtsIMutI CAGTG 4 cut(s) 153, 583, 699, 1258
Cac8I GCNNGC 2 cut(s) 1746, 1868
CaiI CAGNNNCTG 2 cut(s) 470, 743
CciI TCATGA 3 cut(s) 1630, 1711, 1880
CfoI GCGC 3 cut(s) 93, 415, 887
Cfr10I RCCGGY 2 cut(s) 1744, 1842
Cfr13I GGNCC 2 cut(s) 551, 1268
ClaI ATCGAT 1 cut(s) 621
CseI GACGC 1 cut(s) 1585
Csp6I GTAC 7 cut(s) 453, 1206, 1491, 1564, 1626, 1827, 1846
CspAI ACCGGT 1 cut(s) 1842
CviQI GTAC 7 cut(s) 453, 1206, 1491, 1564, 1626, 1827, 1846
DdeI CTNAG 9 cut(s) 246, 295, 447, 520, 1053, 1178, 1343, 1550, 1769
DinI GGCGCC 1 cut(s) 414
DrdI GACNNNNNNGTC 1 cut(s) 50
DseDI GACNNNNNNGTC 1 cut(s) 50
EaeI YGGCCR 1 cut(s) 258
Eam1104I CTCTTC 3 cut(s) 170, 223, 758
EarI CTCTTC 3 cut(s) 170, 223, 758
Ecl136II GAGCTC 1 cut(s) 1670
Eco130I CCWWGG 1 cut(s) 431
Eco24I GRGCYC 1 cut(s) 1672
Eco31I GGTCTC 4 cut(s) 704, 1229, 1426, 1686
Eco32I GATATC 2 cut(s) 619, 1143
Eco47I GGWCC 1 cut(s) 551
Eco47III AGCGCT 1 cut(s) 886
Eco53kI GAGCTC 1 cut(s) 1670
Eco57I CTGAAG 1 cut(s) 1394
Eco91I GGTNACC 1 cut(s) 1256
EcoICRI GAGCTC 1 cut(s) 1670
EcoO65I GGTNACC 1 cut(s) 1256
EcoRI GAATTC 1 cut(s) 1444
EcoRII CCWGG 4 cut(s) 400, 670, 1116, 1468
EcoRV GATATC 2 cut(s) 619, 1143
EcoT14I CCWWGG 1 cut(s) 431
EcoT38I GRGCYC 1 cut(s) 1672
EgeI GGCGCC 1 cut(s) 414
EheI GGCGCC 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 431
FalI AAGNNNNNCTT 2 cut(s) 466, 498
FaqI GGGAC 1 cut(s) 537
FauNDI CATATG 1 cut(s) 852
FbaI TGATCA 1 cut(s) 235
FblI GTMKAC 1 cut(s) 1281
Fnu4HI GCNGC 3 cut(s) 504, 835, 1271
FokI GGATG 6 cut(s) 347, 674, 1485, 1526, 1676, 1753
FriOI GRGCYC 1 cut(s) 1672
Fsp4HI GCNGC 3 cut(s) 504, 835, 1271
FspBI CTAG 6 cut(s) 27, 891, 966, 1392, 1640, 1685
GlaI GCGC 3 cut(s) 92, 414, 886
GluI GCNGC 3 cut(s) 504, 835, 1271
GsuI CTGGAG 2 cut(s) 423, 1139
HaeII RGCGCY 2 cut(s) 416, 888
HaeIII GGCC 4 cut(s) 206, 260, 1270, 1299
HapII CCGG 3 cut(s) 68, 1745, 1843
HgaI GACGC 1 cut(s) 1585
HhaI GCGC 3 cut(s) 93, 415, 887
Hin1I GRCGYC 3 cut(s) 48, 413, 1577
Hin6I GCGC 3 cut(s) 91, 413, 885
HinP1I GCGC 3 cut(s) 91, 413, 885
HincII GTYRAC 2 cut(s) 352, 1401
HindII GTYRAC 2 cut(s) 352, 1401
HindIII AAGCTT 1 cut(s) 757
HinfI GANTC 5 cut(s) 79, 146, 911, 1387, 1715
HpaII CCGG 3 cut(s) 68, 1745, 1843
HphI GGTGA 3 cut(s) 332, 593, 1765
Hpy166II GTNNAC 6 cut(s) 352, 1282, 1401, 1493, 1564, 1756
Hpy188III TCNNGA 7 cut(s) 55, 744, 1441, 1631, 1712, 1838, 1881
Hpy8I GTNNAC 6 cut(s) 352, 1282, 1401, 1493, 1564, 1756
Hpy99I CGWCG 3 cut(s) 50, 53, 56
HpyAV CCTTC 8 cut(s) 196, 988, 1255, 1310, 1358, 1480, 1770, 1811
HpyCH4III ACNGT 5 cut(s) 16, 988, 1024, 1730, 1826
HpyCH4IV ACGT 2 cut(s) 48, 534
HpyCH4V TGCA 8 cut(s) 115, 158, 370, 464, 566, 871, 1620, 1646
HpyF10VI GCNNNNNNNGC 4 cut(s) 251, 765, 1585, 1652
HpyF3I CTNAG 9 cut(s) 246, 295, 447, 520, 1053, 1178, 1343, 1550, 1769
HpySE526I ACGT 2 cut(s) 48, 534
Hsp92I GRCGYC 3 cut(s) 48, 413, 1577
HspAI GCGC 3 cut(s) 91, 413, 885
KasI GGCGCC 1 cut(s) 412
KroI GCCGGC 1 cut(s) 1744
KroNI GCCGGC 1 cut(s) 1746
Ksp22I TGATCA 1 cut(s) 235
LguI GCTCTTC 1 cut(s) 758
LmnI GCTCC 6 cut(s) 30, 251, 882, 905, 1196, 1667
Lsp1109I GCAGC 2 cut(s) 490, 846
LweI GCATC 6 cut(s) 308, 533, 575, 1395, 1731, 1822
MaeI CTAG 6 cut(s) 27, 891, 966, 1392, 1640, 1685
MaeII ACGT 2 cut(s) 48, 534
MaeIII GTNAC 4 cut(s) 581, 664, 1256, 1504
MfeI CAATTG 1 cut(s) 365
MflI RGATCY 2 cut(s) 1113, 1171
MhlI GDGCHC 2 cut(s) 1480, 1672
MlsI TGGCCA 1 cut(s) 260
MluCI AATT 9 cut(s) 129, 265, 342, 365, 1013, 1095, 1188, 1444, 1519
MluNI TGGCCA 1 cut(s) 260
Mly113I GGCGCC 1 cut(s) 413
MlyI GAGTC 2 cut(s) 73, 1709
MmeI TCCRAC 1 cut(s) 1174
Mox20I TGGCCA 1 cut(s) 260
MroNI GCCGGC 1 cut(s) 1744
MroXI GAANNNNTTC 2 cut(s) 751, 1535
MscI TGGCCA 1 cut(s) 260
MseI TTAA 1 cut(s) 1353
MslI CAYNNNNRTG 2 cut(s) 158, 1293
Msp20I TGGCCA 1 cut(s) 260
MspI CCGG 3 cut(s) 68, 1745, 1843
MspR9I CCNGG 4 cut(s) 402, 672, 1118, 1470
MunI CAATTG 1 cut(s) 365
MvaI CCWGG 4 cut(s) 402, 672, 1118, 1470
MvnI CGCG 2 cut(s) 91, 1856
MwoI GCNNNNNNNGC 4 cut(s) 251, 765, 1585, 1652
NaeI GCCGGC 1 cut(s) 1746
NarI GGCGCC 1 cut(s) 413
NdeI CATATG 1 cut(s) 852
NgoMIV GCCGGC 1 cut(s) 1744
NlaIV GGNNCC 3 cut(s) 414, 553, 1479
NmuCI GTSAC 1 cut(s) 581
NspI RCATGY 1 cut(s) 1650
PagI TCATGA 3 cut(s) 1630, 1711, 1880
PciSI GCTCTTC 1 cut(s) 758
PcsI WCGNNNNNNNCGW 4 cut(s) 45, 51, 57, 132
PdiI GCCGGC 1 cut(s) 1746
PdmI GAANNNNTTC 2 cut(s) 751, 1535
PfeI GAWTC 3 cut(s) 146, 911, 1387
PflFI GACNNNGTC 2 cut(s) 47, 50
PflMI CCANNNNNTGG 1 cut(s) 743
PfoI TCCNGGA 3 cut(s) 400, 670, 1116
PinAI ACCGGT 1 cut(s) 1842
PkrI GCNGC 3 cut(s) 505, 836, 1272
PleI GAGTC 2 cut(s) 73, 1709
PluTI GGCGCC 1 cut(s) 416
PpsI GAGTC 2 cut(s) 73, 1709
Psp124BI GAGCTC 1 cut(s) 1672
Psp6I CCWGG 4 cut(s) 400, 670, 1116, 1468
PspEI GGTNACC 1 cut(s) 1256
PspGI CCWGG 4 cut(s) 400, 670, 1116, 1468
PspN4I GGNNCC 3 cut(s) 414, 553, 1479
PspPI GGNCC 2 cut(s) 551, 1268
PstNI CAGNNNCTG 2 cut(s) 470, 743
PsuI RGATCY 2 cut(s) 1113, 1171
PsyI GACNNNGTC 2 cut(s) 47, 50
RsaI GTAC 7 cut(s) 454, 1207, 1492, 1565, 1627, 1828, 1847
RsaNI GTAC 7 cut(s) 453, 1206, 1491, 1564, 1626, 1827, 1846
RseI CAYNNNNRTG 2 cut(s) 158, 1293
SacI GAGCTC 1 cut(s) 1672
SapI GCTCTTC 1 cut(s) 758
SaqAI TTAA 1 cut(s) 1353
SatI GCNGC 3 cut(s) 504, 835, 1271
Sau96I GGNCC 2 cut(s) 551, 1268
ScaI AGTACT 2 cut(s) 454, 1828
SchI GAGTC 2 cut(s) 73, 1709
ScrFI CCNGG 4 cut(s) 402, 672, 1118, 1470
SduI GDGCHC 2 cut(s) 1480, 1672
SfaNI GCATC 6 cut(s) 308, 533, 575, 1395, 1731, 1822
SfcI CTRYAG 1 cut(s) 1822
SfoI GGCGCC 1 cut(s) 414
SinI GGWCC 1 cut(s) 551
SmiMI CAYNNNNRTG 2 cut(s) 158, 1293
SmlI CTYRAG 1 cut(s) 1671
SmoI CTYRAG 1 cut(s) 1671
Sse9I AATT 9 cut(s) 129, 265, 342, 365, 1013, 1095, 1188, 1444, 1519
SsiI CCGC 3 cut(s) 89, 1271, 1854
SspDI GGCGCC 1 cut(s) 412
SspMI CTAG 6 cut(s) 27, 891, 966, 1392, 1640, 1685
SstI GAGCTC 1 cut(s) 1672
StyD4I CCNGG 4 cut(s) 400, 670, 1116, 1468
StyI CCWWGG 1 cut(s) 431
TaaI ACNGT 5 cut(s) 16, 988, 1024, 1730, 1826
TaiI ACGT 2 cut(s) 51, 537
TaqI TCGA 5 cut(s) 60, 126, 540, 621, 1303
TasI AATT 9 cut(s) 129, 265, 342, 365, 1013, 1095, 1188, 1444, 1519
TatI WGTACW 3 cut(s) 452, 1563, 1826
TauI GCSGC 1 cut(s) 1273
TfiI GAWTC 3 cut(s) 146, 911, 1387
Tru1I TTAA 1 cut(s) 1353
Tru9I TTAA 1 cut(s) 1353
TscAI CASTG 4 cut(s) 160, 583, 699, 1258
TseFI GTSAC 1 cut(s) 581
TseI GCWGC 2 cut(s) 503, 834
Tsp45I GTSAC 1 cut(s) 581
TspDTI ATGAA 7 cut(s) 156, 182, 210, 669, 1499, 1528, 1586
TspGWI ACGGA 1 cut(s) 940
TspRI CASTG 4 cut(s) 160, 583, 699, 1258
Tth111I GACNNNGTC 2 cut(s) 47, 50
Van91I CCANNNNNTGG 1 cut(s) 743
VpaK11BI GGWCC 1 cut(s) 551
XapI RAATTY 2 cut(s) 1095, 1444
XceI RCATGY 1 cut(s) 1650
XcmI CCANNNNNNNNNTGG 1 cut(s) 429
XmiI GTMKAC 1 cut(s) 1281
XmnI GAANNNNTTC 2 cut(s) 751, 1535
XspI CTAG 6 cut(s) 27, 891, 966, 1392, 1640, 1685
ZraI GACGTC 1 cut(s) 49
ZrmI AGTACT 2 cut(s) 454, 1828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.