Rh5CG263700

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0003941
Physical Location & Seq
Forward (+)
28147013 .. 28151117
4105 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG263700.1

Sequence Viewer

Length: 1938 bp
ATGGAGTCGCTGCCGTCTCCCCAGAATCCCAAGAAGCGACTGTTTTCGGATACTGTGAGCAGTTCTCCGAGCATGGACGAGGACGACGTCGTCTTGATTCCCCCTCCGGTTACTCGGACTCGGAAATCACTCAAAGGCAAAGAGGCTATTGTGCCGGATGTGATTGAAATTGGTGATGATGAAGATTCTGACACTGCAGTGTTTATGGATAAGAAGAAGGTTCACAGAAGTAATAAAGTGAAGGCCATAAAATACTCTTCCGATGATTACCTTGATCATCGGGCTAAGGGAGCTGTGGCCAATAGTTTTATTAGTTCTGGTAAGAGTTCTTCCTCAGCATCACACAAGGTTGACTATGGTGATTTTTTTTTGGATGAGATAATCGATGTCGATGAGTATACCCGACTGCAATCCCATTTTGATAATGTGGAAATTCCTCCTGGAGTAGAAGCACCTATGCCACCTATCCCCTGGTTGTTGAATCCACCTAAGAGTATTGAAACTGCAGTTTCTGGAAGTAGTTCTTTTGATAGAAACTATCAGCAGGCTGCTAAGAGTTTTGCCTCAGCATCGCATAATGTGATGGACTTAGATGGTCCCAGTTGTGATGCATCTAGTTCCAAGGGTGACTATACTGGTCTTTATTTGAACGAGTTTATAGATATCAATGAGTATGCCCAAATGACGGCTTATTTTGATGAAATGGATGTTCCTCCTGGAATAGAAGCACTTATCCCACACAGTCTCATGTTGTCAGATTTTAGAAGCAGTGTAAAATCAGTTTCTGGAAGTAGTTCAAGCTTAAAGACCCAAAAGGATAGTGTTAGTCACTCTCTTGGGATATCATCATCTTGGAAACTTCCACAAGTTTCCAGCAGTAAAAAGAAACAATCTTATCTGCAACACCAAGGGAATGCTTCTAGTCTCCCAGTGGGAGCAGAATCATCAGTATTTCAAACACAAACTCAAAACAATGTCTCTGTGAAGCCAGTTTCTAGCATGGATACAGAAAAAACTGTGGGGAAGGTAAATGGTTTCAAACAATTTGATACAGTTGAAGATCATTCAGACCATCATTATACTAGGACGAGTCATTTAACAAAGCAGAAGAACTGGGCAAAGAAAATTCAGGAGGAGTGGAAGATCCTGGAGAATGATTTGCCTGATATGATATTCGTTAGAGTTTATGAGACAAGAATGGACCTCTTGAGAGCAGTAATTGTTGGAGCGGAGGGTACTCCTTACCATGATGGTCTCTTTTTCTTTGATATTTCCTTCCCCAGTGGTTATCCCAATGGGCCACCGCATGTCTACTACCACTCTGGTGGCCTTCGACTAAATCCAAATTTGTATGATTGTGGAAAAGTATGCCTCAGCCTTCTCAACACCTGGTCTGGGGGCAAGCAAGAGAAGTGGATCCCTGGTAAGTCAACGATGCTTCAGGTTTTGGTCTCTATACAAGGGCTGATCTTGAATACAGAGCCCTACTTCAATGAGCCTGGATGGGCACCTATGAAGGGTACACCAAGCGGTGAAATGCAATCGAAGAAGTACAATGAGGACACGTTCATCCTATCTCTAAGGACGATGGTGTACATGATGAAGAAGCCTCCAAAGTATTTTGAGGAGTTTGTTATCGGGCATTTCCGCAATCGTGCTCATGACATTCTAAGTGCATGTAAAGCATATATGGATGGAGCTCAAGTGGGGTGTCTAGTCAAAGGTCAGATTCAGGGCGTTAATGGTAACAAGAGCTGCTCACAGGCGTTTAAAAATTCTGTAGCTGGGTTTTTGCCTACTCTTGTCAAACAGTTTACACAGATTGGTGCCAAGGACTGTGAGAAATTTTTACCGCCAACAACAGTCGGGAATAAGCAGAATGGTAGCATGCCTCAGGCTGCAAGACCAACGGTTTCAGAGTGGAAGAAACATTTTTAG

Protein Analysis

645

Amino Acids

71.51

Weight (kDa)

6.54

Isoelectric Point (pI)

42.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 376 - 494 1.5e-20 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 89
AatII GACGTC 1 cut(s) 90
AccB1I GGYRCC 2 cut(s) 1507, 1826
AccBSI CCGCTC 1 cut(s) 1229
AccI GTMKAC 2 cut(s) 398, 1311
AciI CCGC 5 cut(s) 1229, 1304, 1530, 1648, 1853
AclWI GGATC 3 cut(s) 1138, 1411, 1424
AcoI YGGCCR 1 cut(s) 297
AcsI RAATTY 5 cut(s) 432, 1125, 1345, 1774, 1844
AcuI CTGAAG 1 cut(s) 1424
AcyI GRCGYC 1 cut(s) 87
AfaI GTAC 4 cut(s) 1237, 1522, 1553, 1595
AfiI CCNNNNNNNGG 3 cut(s) 685, 1395, 1517
AflIII ACRYGT 1 cut(s) 1563
AjnI CCWGG 7 cut(s) 439, 470, 715, 1146, 1388, 1420, 1498
AleI CACNNNNGTG 2 cut(s) 197, 1323
AluBI AGCT 5 cut(s) 293, 801, 1700, 1755, 1784
AluI AGCT 5 cut(s) 293, 801, 1700, 1755, 1784
Alw21I GWGCWC 2 cut(s) 1660, 1702
Alw26I GTCTC 7 cut(s) 21, 749, 929, 982, 1184, 1259, 1456
AlwI GGATC 3 cut(s) 1138, 1411, 1424
AlwNI CAGNNNCTG 2 cut(s) 512, 785
AoxI GGCC 4 cut(s) 243, 297, 1298, 1327
ApeKI GCWGC 4 cut(s) 10, 548, 1755, 1898
ApoI RAATTY 5 cut(s) 432, 1125, 1345, 1774, 1844
Asp700I GAANNNNTTC 2 cut(s) 520, 793
AspS9I GGNCC 3 cut(s) 596, 1201, 1298
AsuHPI GGTGA 4 cut(s) 185, 371, 638, 1544
AvaII GGWCC 2 cut(s) 596, 1201
AxyI CCTNAGG 1 cut(s) 1893
BaeGI GKGCMC 1 cut(s) 1510
BalI TGGCCA 1 cut(s) 299
BamHI GGATCC 1 cut(s) 1416
BanI GGYRCC 2 cut(s) 1507, 1826
BanII GRGCYC 2 cut(s) 1485, 1702
BarI GAAGNNNNNNTAC 2 cut(s) 313, 345
Bbv12I GWGCWC 2 cut(s) 1660, 1702
BbvCI CCTCAGC 3 cut(s) 334, 565, 1373
BbvI GCAGC 3 cut(s) 535, 1742, 1885
BccI CCATC 7 cut(s) 577, 587, 1080, 1244, 1497, 1582, 1688
BceAI ACGGC 1 cut(s) 702
BciT130I CCWGG 7 cut(s) 441, 472, 717, 1148, 1390, 1422, 1500
BciVI GTATCC 2 cut(s) 43, 997
BclI TGATCA 1 cut(s) 274
BcoDI GTCTC 7 cut(s) 21, 749, 929, 982, 1184, 1259, 1456
BfaI CTAG 5 cut(s) 615, 921, 996, 1083, 1715
BfmI CTRYAG 3 cut(s) 195, 504, 1779
BfuI GTATCC 2 cut(s) 43, 997
BisI GCNGC 4 cut(s) 11, 549, 1756, 1899
BlsI GCNGC 4 cut(s) 12, 550, 1757, 1900
Bme1390I CCNGG 7 cut(s) 441, 472, 717, 1148, 1390, 1422, 1500
Bme18I GGWCC 2 cut(s) 596, 1201
BmgT120I GGNCC 3 cut(s) 596, 1201, 1298
BmiI GGNNCC 4 cut(s) 598, 1418, 1509, 1828
BmrFI CCNGG 7 cut(s) 441, 472, 717, 1148, 1390, 1422, 1500
BmrI ACTGGG 4 cut(s) 594, 923, 1123, 1275
BmsI GCATC 5 cut(s) 347, 578, 598, 620, 1425
BmuI ACTGGG 4 cut(s) 594, 923, 1123, 1275
BplI GAGNNNNNCTC 2 cut(s) 49, 81
BpmI CTGGAG 2 cut(s) 462, 1169
Bpu10I CCTNAGC 4 cut(s) 285, 334, 565, 1373
BpuEI CTTGAG 2 cut(s) 1228, 1686
Bsa29I ATCGAT 1 cut(s) 384
BsaHI GRCGYC 1 cut(s) 87
BsaI GGTCTC 2 cut(s) 1259, 1456
BsaJI CCNNGG 5 cut(s) 470, 621, 907, 1420, 1830
BsaWI WCCGGW 1 cut(s) 106
BsaXI ACNNNNNCTCC 2 cut(s) 1689, 1719
Bsc4I CCNNNNNNNGG 3 cut(s) 685, 1395, 1517
Bse1I ACTGG 6 cut(s) 600, 640, 929, 989, 1118, 1281
Bse21I CCTNAGG 1 cut(s) 1893
BseBI CCWGG 7 cut(s) 441, 472, 717, 1148, 1390, 1422, 1500
BseCI ATCGAT 1 cut(s) 384
BseDI CCNNGG 5 cut(s) 470, 621, 907, 1420, 1830
BseGI GGATG 6 cut(s) 163, 379, 712, 1508, 1569, 1699
BseLI CCNNNNNNNGG 3 cut(s) 685, 1395, 1517
BseMII CTCAG 4 cut(s) 348, 579, 1387, 1907
BseNI ACTGG 6 cut(s) 600, 640, 929, 989, 1118, 1281
BseRI GAGGAG 2 cut(s) 1148, 1640
BseSI GKGCMC 1 cut(s) 1510
BseXI GCAGC 3 cut(s) 535, 1742, 1885
BseYI CCCAGC 1 cut(s) 1784
BshFI GGCC 4 cut(s) 245, 299, 1300, 1329
BshNI GGYRCC 2 cut(s) 1507, 1826
BshVI ATCGAT 1 cut(s) 384
BsiHKAI GWGCWC 2 cut(s) 1660, 1702
BsiSI CCGG 2 cut(s) 107, 155
BslFI GGGAC 1 cut(s) 582
BslI CCNNNNNNNGG 3 cut(s) 685, 1395, 1517
BsmAI GTCTC 7 cut(s) 21, 749, 929, 982, 1184, 1259, 1456
BsmBI CGTCTC 1 cut(s) 21
BsmFI GGGAC 1 cut(s) 582
BsmI GAATGC 1 cut(s) 919
BsnI GGCC 4 cut(s) 245, 299, 1300, 1329
Bso31I GGTCTC 2 cut(s) 1259, 1456
Bsp1286I GDGCHC 4 cut(s) 1485, 1510, 1660, 1702
Bsp1407I TGTACA 1 cut(s) 1593
Bsp143I GATC 5 cut(s) 274, 1060, 1143, 1416, 1467
BspACI CCGC 5 cut(s) 1229, 1304, 1530, 1648, 1853
BspANI GGCC 4 cut(s) 245, 299, 1300, 1329
BspCNI CTCAG 4 cut(s) 347, 578, 1386, 1906
BspDI ATCGAT 1 cut(s) 384
BspHI TCATGA 1 cut(s) 1660
BspLI GGNNCC 4 cut(s) 598, 1418, 1509, 1828
BspMAI CTGCAG 2 cut(s) 199, 508
BspPI GGATC 3 cut(s) 1138, 1411, 1424
BspT107I GGYRCC 2 cut(s) 1507, 1826
BspTNI GGTCTC 2 cut(s) 1259, 1456
BsrBI CCGCTC 1 cut(s) 1229
BsrGI TGTACA 1 cut(s) 1593
BsrI ACTGG 6 cut(s) 600, 640, 929, 989, 1118, 1281
BssECI CCNNGG 5 cut(s) 470, 621, 907, 1420, 1830
BssMI GATC 5 cut(s) 274, 1060, 1143, 1416, 1467
BssNAI GTATAC 1 cut(s) 399
BssNI GRCGYC 1 cut(s) 87
BssT1I CCWWGG 3 cut(s) 621, 907, 1830
Bst1107I GTATAC 1 cut(s) 399
Bst2UI CCWGG 7 cut(s) 441, 472, 717, 1148, 1390, 1422, 1500
Bst4CI ACNGT 9 cut(s) 42, 55, 743, 1018, 1054, 1812, 1838, 1864, 1912
Bst6I CTCTTC 1 cut(s) 262
BstACI GRCGYC 1 cut(s) 87
BstAUI TGTACA 1 cut(s) 1593
BstC8I GCNNGC 3 cut(s) 546, 1403, 1889
BstF5I GGATG 6 cut(s) 163, 379, 712, 1508, 1569, 1699
BstKTI GATC 5 cut(s) 277, 1063, 1146, 1419, 1470
BstMAI GTCTC 7 cut(s) 21, 749, 929, 982, 1184, 1259, 1456
BstMBI GATC 5 cut(s) 274, 1060, 1143, 1416, 1467
BstMWI GCNNNNNNNGC 2 cut(s) 290, 1682
BstNI CCWGG 7 cut(s) 441, 472, 717, 1148, 1390, 1422, 1500
BstNSI RCATGY 3 cut(s) 1310, 1680, 1891
BstSCI CCNGG 7 cut(s) 439, 470, 715, 1146, 1388, 1420, 1498
BstSFI CTRYAG 3 cut(s) 195, 504, 1779
BstSLI GKGCMC 1 cut(s) 1510
BstV1I GCAGC 3 cut(s) 535, 1742, 1885
BstX2I RGATCY 2 cut(s) 1143, 1416
BstXI CCANNNNNNTGG 1 cut(s) 1325
BstYI RGATCY 2 cut(s) 1143, 1416
BstZ17I GTATAC 1 cut(s) 399
Bsu15I ATCGAT 1 cut(s) 384
Bsu36I CCTNAGG 1 cut(s) 1893
BsuI GTATCC 2 cut(s) 43, 997
BsuRI GGCC 4 cut(s) 245, 299, 1300, 1329
BsuTUI ATCGAT 1 cut(s) 384
BtgZI GCGATG 1 cut(s) 555
BtsCI GGATG 6 cut(s) 163, 379, 712, 1508, 1569, 1699
BtsI GCAGTG 3 cut(s) 192, 204, 775
BtsIMutI CAGTG 5 cut(s) 192, 204, 775, 936, 1288
Cac8I GCNNGC 3 cut(s) 546, 1403, 1889
CaiI CAGNNNCTG 2 cut(s) 512, 785
CciI TCATGA 1 cut(s) 1660
Cfr13I GGNCC 3 cut(s) 596, 1201, 1298
ClaI ATCGAT 1 cut(s) 384
CsiI ACCWGGT 1 cut(s) 1388
Csp6I GTAC 4 cut(s) 1236, 1521, 1552, 1594
CspCI CAANNNNNGTGG 2 cut(s) 1394, 1429
CviAII CATG 9 cut(s) 73, 748, 1000, 1247, 1307, 1597, 1661, 1677, 1888
CviQI GTAC 4 cut(s) 1236, 1521, 1552, 1594
DpnI GATC 5 cut(s) 276, 1062, 1145, 1418, 1469
DpnII GATC 5 cut(s) 274, 1060, 1143, 1416, 1467
DraI TTTAAA 1 cut(s) 1771
DrdI GACNNNNNNGTC 1 cut(s) 89
DseDI GACNNNNNNGTC 1 cut(s) 89
EaeI YGGCCR 1 cut(s) 297
Eam1104I CTCTTC 1 cut(s) 262
EarI CTCTTC 1 cut(s) 262
Ecl136II GAGCTC 1 cut(s) 1700
Eco130I CCWWGG 3 cut(s) 621, 907, 1830
Eco24I GRGCYC 2 cut(s) 1485, 1702
Eco31I GGTCTC 2 cut(s) 1259, 1456
Eco32I GATATC 2 cut(s) 664, 843
Eco47I GGWCC 2 cut(s) 596, 1201
Eco53kI GAGCTC 1 cut(s) 1700
Eco57I CTGAAG 1 cut(s) 1424
Eco81I CCTNAGG 1 cut(s) 1893
EcoICRI GAGCTC 1 cut(s) 1700
EcoRII CCWGG 7 cut(s) 439, 470, 715, 1146, 1388, 1420, 1498
EcoRV GATATC 2 cut(s) 664, 843
EcoT14I CCWWGG 3 cut(s) 621, 907, 1830
EcoT22I ATGCAT 1 cut(s) 613
EcoT38I GRGCYC 2 cut(s) 1485, 1702
ErhI CCWWGG 3 cut(s) 621, 907, 1830
Esp3I CGTCTC 1 cut(s) 21
FaeI CATG 9 cut(s) 76, 751, 1003, 1250, 1310, 1600, 1664, 1680, 1891
FalI AAGNNNNNCTT 2 cut(s) 508, 540
FaqI GGGAC 1 cut(s) 582
FatI CATG 9 cut(s) 72, 747, 999, 1246, 1306, 1596, 1660, 1676, 1887
FbaI TGATCA 1 cut(s) 274
FblI GTMKAC 2 cut(s) 398, 1311
Fnu4HI GCNGC 4 cut(s) 11, 549, 1756, 1899
FokI GGATG 6 cut(s) 170, 386, 719, 1515, 1556, 1706
FriOI GRGCYC 2 cut(s) 1485, 1702
Fsp4HI GCNGC 4 cut(s) 11, 549, 1756, 1899
FspBI CTAG 5 cut(s) 615, 921, 996, 1083, 1715
GluI GCNGC 4 cut(s) 11, 549, 1756, 1899
GsaI CCCAGC 1 cut(s) 1788
GsuI CTGGAG 2 cut(s) 462, 1169
HaeIII GGCC 4 cut(s) 245, 299, 1300, 1329
HapII CCGG 2 cut(s) 107, 155
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 9 cut(s) 76, 751, 1003, 1250, 1310, 1600, 1664, 1680, 1891
HincII GTYRAC 2 cut(s) 352, 1431
HindII GTYRAC 2 cut(s) 352, 1431
HindIII AAGCTT 1 cut(s) 799
HinfI GANTC 9 cut(s) 5, 25, 97, 118, 185, 481, 941, 1090, 1729
HpaII CCGG 2 cut(s) 107, 155
HphI GGTGA 4 cut(s) 185, 371, 638, 1544
Hpy166II GTNNAC 8 cut(s) 223, 352, 399, 1312, 1431, 1523, 1594, 1815
Hpy188III TCNNGA 8 cut(s) 94, 513, 786, 1130, 1207, 1471, 1661, 1867
Hpy8I GTNNAC 8 cut(s) 223, 352, 399, 1312, 1431, 1523, 1594, 1815
Hpy99I CGWCG 2 cut(s) 89, 92
HpyAV CCTTC 7 cut(s) 211, 235, 1018, 1285, 1340, 1388, 1510
HpyCH4III ACNGT 9 cut(s) 42, 55, 743, 1018, 1054, 1812, 1838, 1864, 1912
HpyCH4IV ACGT 2 cut(s) 87, 1565
HpyCH4V TGCA 8 cut(s) 197, 409, 506, 611, 901, 1540, 1676, 1901
HpyF10VI GCNNNNNNNGC 2 cut(s) 290, 1682
HpySE526I ACGT 2 cut(s) 87, 1565
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 9 cut(s) 76, 751, 1003, 1250, 1310, 1600, 1664, 1680, 1891
Ksp22I TGATCA 1 cut(s) 274
Kzo9I GATC 5 cut(s) 274, 1060, 1143, 1416, 1467
LmnI GCTCC 4 cut(s) 290, 935, 1226, 1697
Lsp1109I GCAGC 3 cut(s) 535, 1742, 1885
LweI GCATC 5 cut(s) 347, 578, 598, 620, 1425
MabI ACCWGGT 1 cut(s) 1388
MaeI CTAG 5 cut(s) 615, 921, 996, 1083, 1715
MaeII ACGT 2 cut(s) 87, 1565
MaeIII GTNAC 4 cut(s) 109, 626, 827, 1745
MalI GATC 5 cut(s) 276, 1062, 1145, 1418, 1469
MbiI CCGCTC 1 cut(s) 1229
MboI GATC 5 cut(s) 274, 1060, 1143, 1416, 1467
MflI RGATCY 2 cut(s) 1143, 1416
MhlI GDGCHC 4 cut(s) 1485, 1510, 1660, 1702
MlsI TGGCCA 1 cut(s) 299
MluCI AATT 8 cut(s) 168, 432, 1043, 1125, 1218, 1345, 1774, 1844
MluNI TGGCCA 1 cut(s) 299
MlyI GAGTC 3 cut(s) 14, 112, 1099
MmeI TCCRAC 1 cut(s) 1204
Mox20I TGGCCA 1 cut(s) 299
Mph1103I ATGCAT 1 cut(s) 613
MroXI GAANNNNTTC 2 cut(s) 520, 793
MscI TGGCCA 1 cut(s) 299
MseI TTAA 4 cut(s) 803, 1097, 1740, 1770
MslI CAYNNNNRTG 2 cut(s) 197, 1323
Msp20I TGGCCA 1 cut(s) 299
MspI CCGG 2 cut(s) 107, 155
MspR9I CCNGG 7 cut(s) 441, 472, 717, 1148, 1390, 1422, 1500
Mva1269I GAATGC 1 cut(s) 919
MvaI CCWGG 7 cut(s) 441, 472, 717, 1148, 1390, 1422, 1500
MwoI GCNNNNNNNGC 2 cut(s) 290, 1682
NdeII GATC 5 cut(s) 274, 1060, 1143, 1416, 1467
NlaIII CATG 9 cut(s) 76, 751, 1003, 1250, 1310, 1600, 1664, 1680, 1891
NlaIV GGNNCC 4 cut(s) 598, 1418, 1509, 1828
NmuCI GTSAC 2 cut(s) 626, 827
NsiI ATGCAT 1 cut(s) 613
NspI RCATGY 3 cut(s) 1310, 1680, 1891
OliI CACNNNNGTG 2 cut(s) 197, 1323
PaeI GCATGC 1 cut(s) 1891
PagI TCATGA 1 cut(s) 1660
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PctI GAATGC 1 cut(s) 919
PdmI GAANNNNTTC 2 cut(s) 520, 793
PfeI GAWTC 6 cut(s) 25, 97, 185, 481, 941, 1729
PflFI GACNNNGTC 2 cut(s) 86, 89
PfoI TCCNGGA 3 cut(s) 439, 715, 1146
PkrI GCNGC 4 cut(s) 12, 550, 1757, 1900
PleI GAGTC 3 cut(s) 13, 112, 1098
PpsI GAGTC 3 cut(s) 13, 112, 1098
Psp124BI GAGCTC 1 cut(s) 1702
Psp6I CCWGG 7 cut(s) 439, 470, 715, 1146, 1388, 1420, 1498
PspFI CCCAGC 1 cut(s) 1784
PspGI CCWGG 7 cut(s) 439, 470, 715, 1146, 1388, 1420, 1498
PspN4I GGNNCC 4 cut(s) 598, 1418, 1509, 1828
PspPI GGNCC 3 cut(s) 596, 1201, 1298
PstI CTGCAG 2 cut(s) 199, 508
PstNI CAGNNNCTG 2 cut(s) 512, 785
PsuI RGATCY 2 cut(s) 1143, 1416
PsyI GACNNNGTC 2 cut(s) 86, 89
RsaI GTAC 4 cut(s) 1237, 1522, 1553, 1595
RsaNI GTAC 4 cut(s) 1236, 1521, 1552, 1594
RseI CAYNNNNRTG 2 cut(s) 197, 1323
SacI GAGCTC 1 cut(s) 1702
SaqAI TTAA 4 cut(s) 803, 1097, 1740, 1770
SatI GCNGC 4 cut(s) 11, 549, 1756, 1899
Sau3AI GATC 5 cut(s) 274, 1060, 1143, 1416, 1467
Sau96I GGNCC 3 cut(s) 596, 1201, 1298
SchI GAGTC 3 cut(s) 14, 112, 1099
ScrFI CCNGG 7 cut(s) 441, 472, 717, 1148, 1390, 1422, 1500
SduI GDGCHC 4 cut(s) 1485, 1510, 1660, 1702
SexAI ACCWGGT 1 cut(s) 1388
SfaNI GCATC 5 cut(s) 347, 578, 598, 620, 1425
SfcI CTRYAG 3 cut(s) 195, 504, 1779
SinI GGWCC 2 cut(s) 596, 1201
SmiMI CAYNNNNRTG 2 cut(s) 197, 1323
SmlI CTYRAG 2 cut(s) 1207, 1701
SmoI CTYRAG 2 cut(s) 1207, 1701
SphI GCATGC 1 cut(s) 1891
Sse9I AATT 8 cut(s) 168, 432, 1043, 1125, 1218, 1345, 1774, 1844
SsiI CCGC 5 cut(s) 1229, 1304, 1530, 1648, 1853
SspMI CTAG 5 cut(s) 615, 921, 996, 1083, 1715
SstI GAGCTC 1 cut(s) 1702
StyD4I CCNGG 7 cut(s) 439, 470, 715, 1146, 1388, 1420, 1498
StyI CCWWGG 3 cut(s) 621, 907, 1830
TaaI ACNGT 9 cut(s) 42, 55, 743, 1018, 1054, 1812, 1838, 1864, 1912
TaiI ACGT 2 cut(s) 90, 1568
TaqI TCGA 4 cut(s) 384, 390, 1333, 1544
TasI AATT 8 cut(s) 168, 432, 1043, 1125, 1218, 1345, 1774, 1844
TatI WGTACW 2 cut(s) 1551, 1593
TfiI GAWTC 6 cut(s) 25, 97, 185, 481, 941, 1729
Tru1I TTAA 4 cut(s) 803, 1097, 1740, 1770
Tru9I TTAA 4 cut(s) 803, 1097, 1740, 1770
TscAI CASTG 5 cut(s) 199, 204, 775, 936, 1288
TseFI GTSAC 2 cut(s) 626, 827
TseI GCWGC 4 cut(s) 10, 548, 1755, 1898
Tsp45I GTSAC 2 cut(s) 626, 827
TspDTI ATGAA 5 cut(s) 195, 714, 1529, 1558, 1616
TspRI CASTG 5 cut(s) 199, 204, 775, 936, 1288
Tth111I GACNNNGTC 2 cut(s) 86, 89
VpaK11BI GGWCC 2 cut(s) 596, 1201
XapI RAATTY 5 cut(s) 432, 1125, 1345, 1774, 1844
XceI RCATGY 3 cut(s) 1310, 1680, 1891
XcmI CCANNNNNNNNNTGG 1 cut(s) 468
XmiI GTMKAC 2 cut(s) 398, 1311
XmnI GAANNNNTTC 2 cut(s) 520, 793
XspI CTAG 5 cut(s) 615, 921, 996, 1083, 1715
ZraI GACGTC 1 cut(s) 88
Zsp2I ATGCAT 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.