RchiOBHm_Chr5g0033811

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003941
Physical Location & Seq
Forward (+)
27695281 .. 27699777
4497 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31287

Sequence Viewer

Length: 1815 bp
ATGGATGAAGACGACGTCGTCTTGAGTACTCCTCCAATCTCCCGGACTCCGAAATCGGAGAAAGGGAAGGAGGTTATTGTACCTGATGGTATTCACGTCGAAGATTCTGATACTACGGTGTTTATTGATGAGAAAGTTGACAGAAGTATCAAAGGGAAGACAATAAATTACTCTTCCGATGAGTTCCCTGATCGTAAAGCTAAGGGAGCTGTCGCCAATAACTTTGCTGGTTCTTCTGGTAAGAGTTTAGCCTCAGCATCAGATAACATTAACTATGCTGATCTGTTTTTGGATGATGTTATCGATGTCGATGAGTATGCCCGTTTGCTATCCCATTTTGATAATGCGGATATTTCTCCTGGAATAGAAGCACCTATGCCACCTATCCCCTGGTTGTTTGGTCCTGTTAAAAGTGATATAAAGTCAGTTTCTGGAAGTAGTTCTGATACAAATTATCGAGCTGCTAAGAGTTTTGCGTCACTCTCGCATAATGTAATTGACTTAGCTGGTCCCAGTTCTGATGCATCTAGTTCCAATGATGACTATTCTGGTCTTTATCTGGAAGCATCCATAGATATCGATGAGTATGCTAAAATGCTAGCCCATTTTGACAAAATGGATATTCCTCCTGGAATAGAAGCACCTATCCCACATTTTCTGTCATTGTCAGATCCTTCTAAGAGCAATATAAATGCAGTTCCTGGAAGTAGTTCAAGCTTGGAGGCCCAAACGAATGTTTTTAGTCCTGCTCTTGGGCTATCATCATCTTGGAAACTTCCACAAGCTTCCAGCAGTAAAACAAAACAATCTTATCTGCAACACCAAGGGTGGGCTTCTAATATTCCAGTTGGAGTAGAATTATCAAAATTGCATACACAAACTCAAACCAATGATGTCCCTGCAAAGCAAGTTTCTAGCATGGAGACAGATGAAACTATTAGGAAGTTCAAGGATTTCAAACAATTTGATACAGTTGCAGATCACTCAGACCATCACTATACTAACAAGAGTAGTTCTACCATGCAGAAAAATTGGGCAAAGAGAATTCAGGAAGAGTGGAAAATCCTGGAGAAAGATTTGCCTGATACAATATTTGTTAGAGTTTATGAGACAAGGATGGATCTTTTGAAAGCTGTAATTGTTGGAGCCAAGGGTACCCCCTACCATGATGGTCTCTTTTTCTTTGATGTTTCATTTCCAAGTGACTATCCCAATGTACCCCCGCATGTCTACTACCACTCTGGTGGCCTTCGACTAAATCCAAATTTGTATGATTGTGGAAAAGTATGCCTCAGCCTTCTCAACACCTGGTCTGGGGGCAAGCAAGAGATGTGGATCCCTGGTAAGTCAACGATGCTTCAGGTTTTGGTCTCTATACAAGGGCTGATCTTGAATACAGAGCCCTACTTCAATGAGCCTGGATGGGCACCTATGAAGGGTACACCAGCCGGTGAAACGGCATCGAAGAAGTACAATGAGGACACGTTAATCCTATCTCTAAGGACAATGGTGTACACGATGAAGCGGCCTCCAAAGTATTTTGAGCACTTTGTTCTCGGGCATTTCCGCAATCATGCTCATGATATTCTTGTAGCATGCAAGGCATATATGGATGGAGCTGAAGTGGGGTGTTTAGTCAAAGGTTCGGTGCAGGGCGTTGATGGTAATAAGAGCTGCTCGCAGGTGTTTAAGAATTCTCTGGCTAGGTTTTTGCCTACTCTAGTCAAAGAGTTGACACAAATAGGTGCCAAGGACTGTGAGAAATTTGTATCACCAGCAACAGTTGAGAATTTGCAGATTGGTAGCGTGCCTTAG

Protein Analysis

604

Amino Acids

66.42

Weight (kDa)

5.48

Isoelectric Point (pI)

37.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 348 - 467 7.4e-22 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1672
AasI GACNNNNNNGTC 1 cut(s) 17
AatII GACGTC 1 cut(s) 18
Acc36I ACCTGC 1 cut(s) 1672
Acc65I GGTACC 1 cut(s) 1154
AccB1I GGYRCC 3 cut(s) 1154, 1426, 1745
AccI GTMKAC 1 cut(s) 1230
AciI CCGC 4 cut(s) 347, 1223, 1525, 1567
AclWI GGATC 4 cut(s) 665, 1128, 1330, 1343
AcsI RAATTY 5 cut(s) 1044, 1264, 1693, 1763, 1789
AcuI CTGAAG 2 cut(s) 1343, 1641
AcyI GRCGYC 1 cut(s) 15
AfaI GTAC 7 cut(s) 28, 81, 1156, 1218, 1441, 1472, 1514
AfiI CCNNNNNNNGG 4 cut(s) 752, 829, 1314, 1436
AflIII ACRYGT 1 cut(s) 1482
AgsI TTSAA 6 cut(s) 714, 949, 958, 1129, 1393, 1411
AjiI CACGTC 1 cut(s) 97
AjnI CCWGG 8 cut(s) 358, 389, 628, 700, 1065, 1307, 1339, 1417
AleI CACNNNNGTG 1 cut(s) 1242
AluBI AGCT 9 cut(s) 200, 209, 461, 506, 717, 785, 1133, 1619, 1674
AluI AGCT 9 cut(s) 200, 209, 461, 506, 717, 785, 1133, 1619, 1674
Alw21I GWGCWC 1 cut(s) 1548
Alw26I GTCTC 4 cut(s) 917, 1103, 1178, 1375
AlwI GGATC 4 cut(s) 665, 1128, 1330, 1343
AlwNI CAGNNNCTG 2 cut(s) 431, 701
Ama87I CYCGRG 1 cut(s) 1556
AoxI GGCC 3 cut(s) 723, 1246, 1526
ApeKI GCWGC 2 cut(s) 461, 1674
ApoI RAATTY 5 cut(s) 1044, 1264, 1693, 1763, 1789
Asp700I GAANNNNTTC 2 cut(s) 439, 709
Asp718I GGTACC 1 cut(s) 1154
AspS9I GGNCC 3 cut(s) 401, 509, 724
AsuC2I CCSGG 1 cut(s) 43
AsuHPI GGTGA 2 cut(s) 1463, 1764
AsuNHI GCTAGC 1 cut(s) 598
AvaI CYCGRG 1 cut(s) 1556
AvaII GGWCC 2 cut(s) 401, 509
BaeGI GKGCMC 1 cut(s) 1429
BaeI ACNNNNGTAYC 4 cut(s) 102, 130, 135, 163
BamHI GGATCC 1 cut(s) 1335
BanI GGYRCC 3 cut(s) 1154, 1426, 1745
BanII GRGCYC 1 cut(s) 1404
BbsI GAAGAC 2 cut(s) 15, 164
Bbv12I GWGCWC 1 cut(s) 1548
BbvCI CCTCAGC 2 cut(s) 253, 1292
BbvI GCAGC 2 cut(s) 448, 1661
BccI CCATC 7 cut(s) 80, 999, 1111, 1163, 1416, 1607, 1655
BceAI ACGGC 1 cut(s) 1473
BcgI CGANNNNNNTGC 4 cut(s) 299, 333, 569, 603
BciT130I CCWGG 8 cut(s) 360, 391, 630, 702, 1067, 1309, 1341, 1419
BcnI CCSGG 1 cut(s) 43
BcoDI GTCTC 4 cut(s) 917, 1103, 1178, 1375
BfaI CTAG 5 cut(s) 528, 599, 915, 1704, 1721
BfuAI ACCTGC 1 cut(s) 1672
BisI GCNGC 3 cut(s) 462, 1526, 1675
BlsI GCNGC 3 cut(s) 463, 1527, 1676
BmcAI AGTACT 1 cut(s) 28
Bme1390I CCNGG 9 cut(s) 43, 360, 391, 630, 702, 1067, 1309, 1341, 1419
Bme18I GGWCC 2 cut(s) 401, 509
BmeT110I CYCGRG 1 cut(s) 1556
BmgBI CACGTC 1 cut(s) 97
BmgT120I GGNCC 3 cut(s) 401, 509, 724
BmiI GGNNCC 6 cut(s) 511, 1147, 1156, 1337, 1428, 1747
BmrFI CCNGG 9 cut(s) 43, 360, 391, 630, 702, 1067, 1309, 1341, 1419
BmrI ACTGGG 1 cut(s) 507
BmsI GCATC 6 cut(s) 266, 511, 533, 575, 1344, 1469
BmtI GCTAGC 1 cut(s) 602
BmuI ACTGGG 1 cut(s) 507
BpiI GAAGAC 2 cut(s) 15, 164
BplI GAGNNNNNCTC 2 cut(s) 16, 48
BpmI CTGGAG 1 cut(s) 1088
Bpu10I CCTNAGC 3 cut(s) 201, 253, 1292
BpuEI CTTGAG 1 cut(s) 43
BpuMI CCSGG 1 cut(s) 43
Bsa29I ATCGAT 2 cut(s) 303, 579
BsaBI GATNNNNATC 1 cut(s) 1334
BsaHI GRCGYC 1 cut(s) 15
BsaI GGTCTC 2 cut(s) 1178, 1375
BsaJI CCNNGG 5 cut(s) 389, 823, 1149, 1339, 1749
BsaXI ACNNNNNCTCC 2 cut(s) 1608, 1638
Bsc4I CCNNNNNNNGG 4 cut(s) 752, 829, 1314, 1436
Bse118I RCCGGY 1 cut(s) 1448
Bse1I ACTGG 2 cut(s) 513, 845
Bse8I GATNNNNATC 1 cut(s) 1334
BseBI CCWGG 8 cut(s) 360, 391, 630, 702, 1067, 1309, 1341, 1419
BseCI ATCGAT 2 cut(s) 303, 579
BseDI CCNNGG 5 cut(s) 389, 823, 1149, 1339, 1749
BseGI GGATG 6 cut(s) 10, 298, 566, 1122, 1427, 1618
BseJI GATNNNNATC 1 cut(s) 1334
BseLI CCNNNNNNNGG 4 cut(s) 752, 829, 1314, 1436
BseMII CTCAG 3 cut(s) 267, 999, 1306
BseNI ACTGG 2 cut(s) 513, 845
BseRI GAGGAG 1 cut(s) 21
BseSI GKGCMC 1 cut(s) 1429
BseXI GCAGC 2 cut(s) 448, 1661
BsgI GTGCAG 1 cut(s) 1670
BshFI GGCC 3 cut(s) 725, 1248, 1528
BshNI GGYRCC 3 cut(s) 1154, 1426, 1745
BshVI ATCGAT 2 cut(s) 303, 579
BsiHKAI GWGCWC 1 cut(s) 1548
BsiHKCI CYCGRG 1 cut(s) 1556
BsiSI CCGG 2 cut(s) 43, 1449
BslFI GGGAC 2 cut(s) 495, 881
BslI CCNNNNNNNGG 4 cut(s) 752, 829, 1314, 1436
BsmAI GTCTC 4 cut(s) 917, 1103, 1178, 1375
BsmFI GGGAC 2 cut(s) 495, 881
BsnI GGCC 3 cut(s) 725, 1248, 1528
Bso31I GGTCTC 2 cut(s) 1178, 1375
BsoBI CYCGRG 1 cut(s) 1556
Bsp1286I GDGCHC 3 cut(s) 1404, 1429, 1548
Bsp1407I TGTACA 1 cut(s) 1512
Bsp143I GATC 7 cut(s) 190, 280, 670, 979, 1120, 1335, 1386
BspACI CCGC 4 cut(s) 347, 1223, 1525, 1567
BspANI GGCC 3 cut(s) 725, 1248, 1528
BspCNI CTCAG 3 cut(s) 266, 998, 1305
BspDI ATCGAT 2 cut(s) 303, 579
BspHI TCATGA 1 cut(s) 1579
BspLI GGNNCC 6 cut(s) 511, 1147, 1156, 1337, 1428, 1747
BspMI ACCTGC 1 cut(s) 1672
BspOI GCTAGC 1 cut(s) 602
BspPI GGATC 4 cut(s) 665, 1128, 1330, 1343
BspT107I GGYRCC 3 cut(s) 1154, 1426, 1745
BspTNI GGTCTC 2 cut(s) 1178, 1375
BsrFI RCCGGY 1 cut(s) 1448
BsrGI TGTACA 1 cut(s) 1512
BsrI ACTGG 2 cut(s) 513, 845
BssAI RCCGGY 1 cut(s) 1448
BssECI CCNNGG 5 cut(s) 389, 823, 1149, 1339, 1749
BssMI GATC 7 cut(s) 190, 280, 670, 979, 1120, 1335, 1386
BssNI GRCGYC 1 cut(s) 15
BssT1I CCWWGG 3 cut(s) 823, 1149, 1749
Bst2UI CCWGG 8 cut(s) 360, 391, 630, 702, 1067, 1309, 1341, 1419
Bst4CI ACNGT 4 cut(s) 118, 973, 1757, 1783
Bst6I CTCTTC 2 cut(s) 178, 1047
BstACI GRCGYC 1 cut(s) 15
BstAUI TGTACA 1 cut(s) 1512
BstC8I GCNNGC 5 cut(s) 600, 1322, 1597, 1679, 1808
BstDEI CTNAG 9 cut(s) 201, 253, 465, 502, 678, 985, 1292, 1499, 1812
BstF5I GGATG 6 cut(s) 10, 298, 566, 1122, 1427, 1618
BstKTI GATC 7 cut(s) 193, 283, 673, 982, 1123, 1338, 1389
BstMAI GTCTC 4 cut(s) 917, 1103, 1178, 1375
BstMBI GATC 7 cut(s) 190, 280, 670, 979, 1120, 1335, 1386
BstMWI GCNNNNNNNGC 2 cut(s) 206, 1601
BstNI CCWGG 8 cut(s) 360, 391, 630, 702, 1067, 1309, 1341, 1419
BstNSI RCATGY 2 cut(s) 1229, 1599
BstSCI CCNGG 9 cut(s) 41, 358, 389, 628, 700, 1065, 1307, 1339, 1417
BstSLI GKGCMC 1 cut(s) 1429
BstV1I GCAGC 2 cut(s) 448, 1661
BstV2I GAAGAC 2 cut(s) 15, 164
BstX2I RGATCY 3 cut(s) 670, 1120, 1335
BstXI CCANNNNNNTGG 1 cut(s) 1244
BstYI RGATCY 3 cut(s) 670, 1120, 1335
Bsu15I ATCGAT 2 cut(s) 303, 579
BsuRI GGCC 3 cut(s) 725, 1248, 1528
BsuTUI ATCGAT 2 cut(s) 303, 579
BtrI CACGTC 1 cut(s) 97
BtsCI GGATG 6 cut(s) 10, 298, 566, 1122, 1427, 1618
BveI ACCTGC 1 cut(s) 1672
Cac8I GCNNGC 5 cut(s) 600, 1322, 1597, 1679, 1808
CaiI CAGNNNCTG 2 cut(s) 431, 701
CciI TCATGA 1 cut(s) 1579
Cfr10I RCCGGY 1 cut(s) 1448
Cfr13I GGNCC 3 cut(s) 401, 509, 724
ClaI ATCGAT 2 cut(s) 303, 579
CseI GACGC 1 cut(s) 465
CsiI ACCWGGT 1 cut(s) 1307
Csp6I GTAC 7 cut(s) 27, 80, 1155, 1217, 1440, 1471, 1513
CspCI CAANNNNNGTGG 2 cut(s) 1313, 1348
CviAII CATG 7 cut(s) 919, 1021, 1166, 1226, 1574, 1580, 1596
CviQI GTAC 7 cut(s) 27, 80, 1155, 1217, 1440, 1471, 1513
DdeI CTNAG 9 cut(s) 201, 253, 465, 502, 678, 985, 1292, 1499, 1812
DpnI GATC 7 cut(s) 192, 282, 672, 981, 1122, 1337, 1388
DpnII GATC 7 cut(s) 190, 280, 670, 979, 1120, 1335, 1386
DrdI GACNNNNNNGTC 1 cut(s) 17
DseDI GACNNNNNNGTC 1 cut(s) 17
Eam1104I CTCTTC 2 cut(s) 178, 1047
EarI CTCTTC 2 cut(s) 178, 1047
Eco130I CCWWGG 3 cut(s) 823, 1149, 1749
Eco24I GRGCYC 1 cut(s) 1404
Eco31I GGTCTC 2 cut(s) 1178, 1375
Eco32I GATATC 1 cut(s) 577
Eco47I GGWCC 2 cut(s) 401, 509
Eco57I CTGAAG 2 cut(s) 1343, 1641
Eco88I CYCGRG 1 cut(s) 1556
EcoRI GAATTC 2 cut(s) 1044, 1693
EcoRII CCWGG 8 cut(s) 358, 389, 628, 700, 1065, 1307, 1339, 1417
EcoRV GATATC 1 cut(s) 577
EcoT14I CCWWGG 3 cut(s) 823, 1149, 1749
EcoT22I ATGCAT 1 cut(s) 526
EcoT38I GRGCYC 1 cut(s) 1404
ErhI CCWWGG 3 cut(s) 823, 1149, 1749
FaeI CATG 7 cut(s) 922, 1024, 1169, 1229, 1577, 1583, 1599
FaqI GGGAC 2 cut(s) 495, 881
FatI CATG 7 cut(s) 918, 1020, 1165, 1225, 1573, 1579, 1595
FauI CCCGC 1 cut(s) 1230
FblI GTMKAC 1 cut(s) 1230
Fnu4HI GCNGC 3 cut(s) 462, 1526, 1675
FokI GGATG 6 cut(s) 17, 305, 553, 1129, 1434, 1625
FriOI GRGCYC 1 cut(s) 1404
Fsp4HI GCNGC 3 cut(s) 462, 1526, 1675
FspBI CTAG 5 cut(s) 528, 599, 915, 1704, 1721
GluI GCNGC 3 cut(s) 462, 1526, 1675
GsuI CTGGAG 1 cut(s) 1088
HaeIII GGCC 3 cut(s) 725, 1248, 1528
HapII CCGG 2 cut(s) 43, 1449
HgaI GACGC 1 cut(s) 465
Hin1I GRCGYC 1 cut(s) 15
Hin1II CATG 7 cut(s) 922, 1024, 1169, 1229, 1577, 1583, 1599
HincII GTYRAC 3 cut(s) 139, 1350, 1734
HindII GTYRAC 3 cut(s) 139, 1350, 1734
HindIII AAGCTT 2 cut(s) 715, 783
HinfI GANTC 2 cut(s) 46, 104
HpaII CCGG 2 cut(s) 43, 1449
HphI GGTGA 2 cut(s) 1463, 1764
Hpy166II GTNNAC 7 cut(s) 139, 1231, 1350, 1442, 1513, 1515, 1734
Hpy188I TCNGA 9 cut(s) 51, 58, 109, 178, 262, 445, 520, 670, 988
Hpy188III TCNNGA 6 cut(s) 22, 432, 560, 1049, 1390, 1580
Hpy8I GTNNAC 7 cut(s) 139, 1231, 1350, 1442, 1513, 1515, 1734
Hpy99I CGWCG 3 cut(s) 17, 20, 101
HpyAV CCTTC 5 cut(s) 61, 684, 1259, 1307, 1429
HpyCH4III ACNGT 4 cut(s) 118, 973, 1757, 1783
HpyCH4IV ACGT 3 cut(s) 15, 96, 1484
HpyF10VI GCNNNNNNNGC 2 cut(s) 206, 1601
HpyF3I CTNAG 9 cut(s) 201, 253, 465, 502, 678, 985, 1292, 1499, 1812
HpySE526I ACGT 3 cut(s) 15, 96, 1484
Hsp92I GRCGYC 1 cut(s) 15
Hsp92II CATG 7 cut(s) 922, 1024, 1169, 1229, 1577, 1583, 1599
KpnI GGTACC 1 cut(s) 1158
Kzo9I GATC 7 cut(s) 190, 280, 670, 979, 1120, 1335, 1386
LmnI GCTCC 3 cut(s) 206, 1145, 1616
Lsp1109I GCAGC 2 cut(s) 448, 1661
LweI GCATC 6 cut(s) 266, 511, 533, 575, 1344, 1469
MabI ACCWGGT 1 cut(s) 1307
MaeI CTAG 5 cut(s) 528, 599, 915, 1704, 1721
MaeII ACGT 3 cut(s) 15, 96, 1484
MaeIII GTNAC 2 cut(s) 477, 1202
MalI GATC 7 cut(s) 192, 282, 672, 981, 1122, 1337, 1388
MboI GATC 7 cut(s) 190, 280, 670, 979, 1120, 1335, 1386
MboII GAAGA 7 cut(s) 20, 113, 165, 169, 225, 1064, 1477
MflI RGATCY 3 cut(s) 670, 1120, 1335
MhlI GDGCHC 3 cut(s) 1404, 1429, 1548
MlyI GAGTC 1 cut(s) 40
MmeI TCCRAC 2 cut(s) 829, 1123
MnlI CCTC 8 cut(s) 42, 64, 262, 636, 715, 1301, 1471, 1539
Mph1103I ATGCAT 1 cut(s) 526
MroXI GAANNNNTTC 2 cut(s) 439, 709
MseI TTAA 4 cut(s) 270, 408, 1487, 1689
MslI CAYNNNNRTG 2 cut(s) 1242, 1578
MspI CCGG 2 cut(s) 43, 1449
MspR9I CCNGG 9 cut(s) 43, 360, 391, 630, 702, 1067, 1309, 1341, 1419
MvaI CCWGG 8 cut(s) 360, 391, 630, 702, 1067, 1309, 1341, 1419
MwoI GCNNNNNNNGC 2 cut(s) 206, 1601
NciI CCSGG 1 cut(s) 43
NdeII GATC 7 cut(s) 190, 280, 670, 979, 1120, 1335, 1386
NheI GCTAGC 1 cut(s) 598
NlaIII CATG 7 cut(s) 922, 1024, 1169, 1229, 1577, 1583, 1599
NlaIV GGNNCC 6 cut(s) 511, 1147, 1156, 1337, 1428, 1747
NmuCI GTSAC 2 cut(s) 477, 1202
NsiI ATGCAT 1 cut(s) 526
NspI RCATGY 2 cut(s) 1229, 1599
OliI CACNNNNGTG 1 cut(s) 1242
PaeI GCATGC 1 cut(s) 1599
PagI TCATGA 1 cut(s) 1579
PaqCI CACCTGC 1 cut(s) 1672
PdmI GAANNNNTTC 2 cut(s) 439, 709
PfeI GAWTC 1 cut(s) 104
PflFI GACNNNGTC 2 cut(s) 14, 17
PfoI TCCNGGA 5 cut(s) 41, 358, 628, 700, 1065
PkrI GCNGC 3 cut(s) 463, 1527, 1676
PleI GAGTC 1 cut(s) 40
PpsI GAGTC 1 cut(s) 40
Psp6I CCWGG 8 cut(s) 358, 389, 628, 700, 1065, 1307, 1339, 1417
PspGI CCWGG 8 cut(s) 358, 389, 628, 700, 1065, 1307, 1339, 1417
PspN4I GGNNCC 6 cut(s) 511, 1147, 1156, 1337, 1428, 1747
PspPI GGNCC 3 cut(s) 401, 509, 724
PstNI CAGNNNCTG 2 cut(s) 431, 701
PsuI RGATCY 3 cut(s) 670, 1120, 1335
PsyI GACNNNGTC 2 cut(s) 14, 17
RsaI GTAC 7 cut(s) 28, 81, 1156, 1218, 1441, 1472, 1514
RsaNI GTAC 7 cut(s) 27, 80, 1155, 1217, 1440, 1471, 1513
RseI CAYNNNNRTG 2 cut(s) 1242, 1578
SaqAI TTAA 4 cut(s) 270, 408, 1487, 1689
SatI GCNGC 3 cut(s) 462, 1526, 1675
Sau3AI GATC 7 cut(s) 190, 280, 670, 979, 1120, 1335, 1386
Sau96I GGNCC 3 cut(s) 401, 509, 724
ScaI AGTACT 1 cut(s) 28
SchI GAGTC 1 cut(s) 40
ScrFI CCNGG 9 cut(s) 43, 360, 391, 630, 702, 1067, 1309, 1341, 1419
SduI GDGCHC 3 cut(s) 1404, 1429, 1548
SexAI ACCWGGT 1 cut(s) 1307
SfaNI GCATC 6 cut(s) 266, 511, 533, 575, 1344, 1469
SinI GGWCC 2 cut(s) 401, 509
SmiMI CAYNNNNRTG 2 cut(s) 1242, 1578
SmlI CTYRAG 1 cut(s) 22
SmoI CTYRAG 1 cut(s) 22
SphI GCATGC 1 cut(s) 1599
SsiI CCGC 4 cut(s) 347, 1223, 1525, 1567
SspI AATATT 2 cut(s) 841, 1092
SspMI CTAG 5 cut(s) 528, 599, 915, 1704, 1721
StyD4I CCNGG 9 cut(s) 41, 358, 389, 628, 700, 1065, 1307, 1339, 1417
StyI CCWWGG 3 cut(s) 823, 1149, 1749
TaaI ACNGT 4 cut(s) 118, 973, 1757, 1783
TaiI ACGT 3 cut(s) 18, 99, 1487
TaqI TCGA 7 cut(s) 99, 303, 309, 457, 579, 1252, 1463
TatI WGTACW 3 cut(s) 26, 1470, 1512
TauI GCSGC 1 cut(s) 1528
TfiI GAWTC 1 cut(s) 104
Tru1I TTAA 4 cut(s) 270, 408, 1487, 1689
Tru9I TTAA 4 cut(s) 270, 408, 1487, 1689
TseFI GTSAC 2 cut(s) 477, 1202
TseI GCWGC 2 cut(s) 461, 1674
Tsp45I GTSAC 2 cut(s) 477, 1202
TspDTI ATGAA 5 cut(s) 21, 945, 1182, 1448, 1535
Tth111I GACNNNGTC 2 cut(s) 14, 17
VpaK11BI GGWCC 2 cut(s) 401, 509
XapI RAATTY 5 cut(s) 1044, 1264, 1693, 1763, 1789
XceI RCATGY 2 cut(s) 1229, 1599
XcmI CCANNNNNNNNNTGG 1 cut(s) 387
XmiI GTMKAC 1 cut(s) 1230
XmnI GAANNNNTTC 2 cut(s) 439, 709
XspI CTAG 5 cut(s) 528, 599, 915, 1704, 1721
ZraI GACGTC 1 cut(s) 16
ZrmI AGTACT 1 cut(s) 28
Zsp2I ATGCAT 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.