Rh5BG233900

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003941
Physical Location & Seq
Forward (+)
27793962 .. 27798877
4916 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG233900.1

Sequence Viewer

Length: 1926 bp
ATGGAGTCGCCGCCGTTCCCCCATATTTCCGAGAAGCCAGTGTTTCCGGAGAGCAGTTCTCAGTGCATGGATGAAGACGACGTCGTCTTGAGTACTCCTCCAATCTCCCGGACTCCGAAATCCGAGAAAGGGAAGGAGGTTATTGTACCTGATGGTATTCACTTCGAAGATTCTGATACTACGGTGTTTATTGATGAGAAAGTTGACAGAAGTATCAAAGGGAAGACAATAAATTACTCTTCCGATGAGTTCCCTGATCGTAAAGCTAAGGGAGCTGTCGCCAATAACTTTGCTGGTTCTTCTGGTAAGAGTTTAGCCTCAGCATCGGATAACATTAACTATGCTGATCTGTTTTTGGATGATGTTATCGATGTCGATGAGTATGCCCGTTTGCTATCCCATTTTGATAATGCGGATATTTCTCCTGGAATAGAAGCACCTATGCCACCTATCCCCTGGTTGTTTGGTCCTGTTAAAAGTGATATAAAGTCAGTTTCTGGAAGTAGTTCTGATACAAATTATCGAGCTGCTAAGAGTTTTGCGTCACTCTCGCATAATGTAATTGACTTAGCTGGTCCCAGTTCTGATGCATCTAGTTCCAATGATGACTATTCTGGTCTTTATTTGGAAGCGTCCATAGATATCGATGAGTATGCTGAAATGCTAGCCCATTTTGACAAAATGGATATTCCTCCTGGAATAGAAGCACCTATCCCACATTTTCTGTCATTGTCAGATCCTTCTAAGAGCAATATAAATGCAGTTCCTGGAAGTAGTTCAAGCTTGGAGGCCCAAACAAATGTTTTTAGTCCTGCTCTTGGGCTATCATCATCTTGGAAACTTCCACAAGCTTCCAGCAGTAAAACAAAACAATCTTATCTGCAACACCAAGGGTGGGCTTCTAATATTCCAGTTGGAGTAGAATTATCAAAATTGCATACACAAACTCAAACCAATGATGTCCCTGCAAAGCAAGTTTCTAGCATGGAGACAGATGAAACTATTAGGAAGTTCAAGGATTTCAAACAATTTGATACAGTTGCAGATCACTCAGACCATCACTATACTAACAAGAGTAGTTCTACCATGCAGAAAAATTGGGCAAAGAGAATTCAGGAAGAGTGGAAAATCCTGGAGAAAGATTTGCCTGATACAATATTTGTTAGAGTTTATGAGACAAGGATGGATCTTTTGAGAGCTGTAATTGTTGGAGCCAAGGGTACCCCCTACCATGATGGTCTCTTTTTCTTTGATGTTTCATTTCCAAGTGACTATCCCAATGTACCCCCGCATGTCTACTACCACTCTGGTGGCCTTCGACTAAATCCAAATTTGTATGATTGTGGAAAAGTATGCCTCAGCCTTCTCAACACCTGGTCTGGGGGCAAGCAAGAGATGTGGATCCCTGGTAAGTCAACGATGCTTCAGGTTTTGGTCTCTATACAAGGGCTGATCTTGAATACAGAGCCCTACTTCAATGAGCCTGGATGGGCACCTATGAAGGGTACACCAGCCGGTGAAATGGCATCGAAGAAGTACAATGAGGACACGTTAATCCTATCTCTAAGGACAATGGTGTACATGATGAAGCGGCCTCCAAAGTATTTTGAGCACTTTGTTCTTGGGCATTTCCGCAATCATGCTCATGATATTCTTGTAGCATGCAAGGCATATATGGATGGAGCTGAAGTGGGGTGTTTAGTCAAAGGTTTGGTTCAGGGCGTTGATGGTAATAAGAGCTGCTCGCAGGTGTATAAGAATTCTCTGGCTAGGTTTTTGCCTACTCTAGTCAAAGAGTTGACACAAATAGGTGCCAAGGACTGTGAGAAATTTGTATCACCAGCAACAGTTGAGAATTTGCAGATTGGTAGCATGCCTCAGGCTGTAACACCAACACAGGTTTCATGGTGGAAGAAACAGTTTTAA

Protein Analysis

641

Amino Acids

70.89

Weight (kDa)

5.43

Isoelectric Point (pI)

42.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 370 - 489 1.8e-21 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1738
AasI GACNNNNNNGTC 1 cut(s) 83
AatII GACGTC 1 cut(s) 84
Acc36I ACCTGC 1 cut(s) 1738
Acc65I GGTACC 1 cut(s) 1220
AccB1I GGYRCC 3 cut(s) 1220, 1492, 1811
AccI GTMKAC 1 cut(s) 1296
AccIII TCCGGA 1 cut(s) 46
AciI CCGC 5 cut(s) 11, 413, 1289, 1591, 1633
AclWI GGATC 4 cut(s) 731, 1194, 1396, 1409
AcsI RAATTY 5 cut(s) 1110, 1330, 1759, 1829, 1855
AcuI CTGAAG 2 cut(s) 1409, 1707
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 7 cut(s) 94, 147, 1222, 1284, 1507, 1538, 1580
AfiI CCNNNNNNNGG 5 cut(s) 129, 818, 895, 1380, 1502
AflIII ACRYGT 1 cut(s) 1548
AgsI TTSAA 5 cut(s) 780, 1015, 1024, 1459, 1477
AjnI CCWGG 8 cut(s) 424, 455, 694, 766, 1131, 1373, 1405, 1483
AjuI GAANNNNNNNTTGG 2 cut(s) 1885, 1917
AleI CACNNNNGTG 1 cut(s) 1308
AluBI AGCT 9 cut(s) 266, 275, 527, 572, 783, 851, 1199, 1685, 1740
AluI AGCT 9 cut(s) 266, 275, 527, 572, 783, 851, 1199, 1685, 1740
Alw21I GWGCWC 1 cut(s) 1614
Alw26I GTCTC 4 cut(s) 983, 1169, 1244, 1441
AlwI GGATC 4 cut(s) 731, 1194, 1396, 1409
AlwNI CAGNNNCTG 2 cut(s) 497, 767
Aor13HI TCCGGA 1 cut(s) 46
AoxI GGCC 3 cut(s) 789, 1312, 1592
ApeKI GCWGC 2 cut(s) 527, 1740
ApoI RAATTY 5 cut(s) 1110, 1330, 1759, 1829, 1855
Asp700I GAANNNNTTC 2 cut(s) 505, 775
Asp718I GGTACC 1 cut(s) 1220
AspS9I GGNCC 3 cut(s) 467, 575, 790
AsuC2I CCSGG 1 cut(s) 109
AsuHPI GGTGA 2 cut(s) 1529, 1830
AsuII TTCGAA 1 cut(s) 165
AsuNHI GCTAGC 1 cut(s) 664
AvaII GGWCC 2 cut(s) 467, 575
AxyI CCTNAGG 1 cut(s) 1878
BaeGI GKGCMC 1 cut(s) 1495
BaeI ACNNNNGTAYC 4 cut(s) 168, 196, 201, 229
BamHI GGATCC 1 cut(s) 1401
BanI GGYRCC 3 cut(s) 1220, 1492, 1811
BanII GRGCYC 1 cut(s) 1470
BbsI GAAGAC 2 cut(s) 81, 230
Bbv12I GWGCWC 1 cut(s) 1614
BbvCI CCTCAGC 2 cut(s) 319, 1358
BbvI GCAGC 2 cut(s) 514, 1727
BccI CCATC 7 cut(s) 146, 1065, 1177, 1229, 1482, 1673, 1721
BcgI CGANNNNNNTGC 4 cut(s) 365, 399, 635, 669
BciT130I CCWGG 8 cut(s) 426, 457, 696, 768, 1133, 1375, 1407, 1485
BcnI CCSGG 1 cut(s) 109
BcoDI GTCTC 4 cut(s) 983, 1169, 1244, 1441
BfaI CTAG 5 cut(s) 594, 665, 981, 1770, 1787
BfuAI ACCTGC 1 cut(s) 1738
BisI GCNGC 4 cut(s) 11, 528, 1592, 1741
BlsI GCNGC 4 cut(s) 12, 529, 1593, 1742
BmcAI AGTACT 1 cut(s) 94
Bme1390I CCNGG 9 cut(s) 109, 426, 457, 696, 768, 1133, 1375, 1407, 1485
Bme18I GGWCC 2 cut(s) 467, 575
BmgT120I GGNCC 3 cut(s) 467, 575, 790
BmiI GGNNCC 6 cut(s) 577, 1213, 1222, 1403, 1494, 1813
BmrFI CCNGG 9 cut(s) 109, 426, 457, 696, 768, 1133, 1375, 1407, 1485
BmrI ACTGGG 1 cut(s) 573
BmsI GCATC 5 cut(s) 332, 577, 599, 1410, 1535
BmtI GCTAGC 1 cut(s) 668
BmuI ACTGGG 1 cut(s) 573
BpiI GAAGAC 2 cut(s) 81, 230
BplI GAGNNNNNCTC 4 cut(s) 43, 75, 82, 114
BpmI CTGGAG 1 cut(s) 1154
Bpu10I CCTNAGC 3 cut(s) 267, 319, 1358
Bpu14I TTCGAA 1 cut(s) 165
BpuEI CTTGAG 1 cut(s) 109
BpuMI CCSGG 1 cut(s) 109
Bsa29I ATCGAT 2 cut(s) 369, 645
BsaBI GATNNNNATC 1 cut(s) 1400
BsaHI GRCGYC 1 cut(s) 81
BsaI GGTCTC 2 cut(s) 1244, 1441
BsaJI CCNNGG 5 cut(s) 455, 889, 1215, 1405, 1815
BsaWI WCCGGW 1 cut(s) 46
BsaXI ACNNNNNCTCC 2 cut(s) 1674, 1704
Bsc4I CCNNNNNNNGG 5 cut(s) 129, 818, 895, 1380, 1502
Bse118I RCCGGY 1 cut(s) 1514
Bse1I ACTGG 3 cut(s) 38, 579, 911
Bse21I CCTNAGG 1 cut(s) 1878
Bse8I GATNNNNATC 1 cut(s) 1400
BseAI TCCGGA 1 cut(s) 46
BseBI CCWGG 8 cut(s) 426, 457, 696, 768, 1133, 1375, 1407, 1485
BseCI ATCGAT 2 cut(s) 369, 645
BseDI CCNNGG 5 cut(s) 455, 889, 1215, 1405, 1815
BseGI GGATG 5 cut(s) 76, 364, 1188, 1493, 1684
BseJI GATNNNNATC 1 cut(s) 1400
BseLI CCNNNNNNNGG 5 cut(s) 129, 818, 895, 1380, 1502
BseMII CTCAG 5 cut(s) 74, 333, 1065, 1372, 1892
BseNI ACTGG 3 cut(s) 38, 579, 911
BseRI GAGGAG 1 cut(s) 87
BseSI GKGCMC 1 cut(s) 1495
BseXI GCAGC 2 cut(s) 514, 1727
BshFI GGCC 3 cut(s) 791, 1314, 1594
BshNI GGYRCC 3 cut(s) 1220, 1492, 1811
BshVI ATCGAT 2 cut(s) 369, 645
BsiHKAI GWGCWC 1 cut(s) 1614
BsiSI CCGG 3 cut(s) 47, 109, 1515
BslFI GGGAC 2 cut(s) 561, 947
BslI CCNNNNNNNGG 5 cut(s) 129, 818, 895, 1380, 1502
BsmAI GTCTC 4 cut(s) 983, 1169, 1244, 1441
BsmFI GGGAC 2 cut(s) 561, 947
BsnI GGCC 3 cut(s) 791, 1314, 1594
Bso31I GGTCTC 2 cut(s) 1244, 1441
Bsp119I TTCGAA 1 cut(s) 165
Bsp1286I GDGCHC 3 cut(s) 1470, 1495, 1614
Bsp13I TCCGGA 1 cut(s) 46
Bsp1407I TGTACA 1 cut(s) 1578
Bsp143I GATC 7 cut(s) 256, 346, 736, 1045, 1186, 1401, 1452
BspACI CCGC 5 cut(s) 11, 413, 1289, 1591, 1633
BspANI GGCC 3 cut(s) 791, 1314, 1594
BspCNI CTCAG 5 cut(s) 73, 332, 1064, 1371, 1891
BspDI ATCGAT 2 cut(s) 369, 645
BspEI TCCGGA 1 cut(s) 46
BspHI TCATGA 1 cut(s) 1645
BspLI GGNNCC 6 cut(s) 577, 1213, 1222, 1403, 1494, 1813
BspMI ACCTGC 1 cut(s) 1738
BspOI GCTAGC 1 cut(s) 668
BspPI GGATC 4 cut(s) 731, 1194, 1396, 1409
BspT104I TTCGAA 1 cut(s) 165
BspT107I GGYRCC 3 cut(s) 1220, 1492, 1811
BspTNI GGTCTC 2 cut(s) 1244, 1441
BsrFI RCCGGY 1 cut(s) 1514
BsrGI TGTACA 1 cut(s) 1578
BsrI ACTGG 3 cut(s) 38, 579, 911
BssAI RCCGGY 1 cut(s) 1514
BssECI CCNNGG 5 cut(s) 455, 889, 1215, 1405, 1815
BssMI GATC 7 cut(s) 256, 346, 736, 1045, 1186, 1401, 1452
BssNI GRCGYC 1 cut(s) 81
BssT1I CCWWGG 3 cut(s) 889, 1215, 1815
Bst2UI CCWGG 8 cut(s) 426, 457, 696, 768, 1133, 1375, 1407, 1485
Bst4CI ACNGT 5 cut(s) 184, 1039, 1823, 1849, 1920
Bst6I CTCTTC 2 cut(s) 244, 1113
BstACI GRCGYC 1 cut(s) 81
BstAUI TGTACA 1 cut(s) 1578
BstBI TTCGAA 1 cut(s) 165
BstC8I GCNNGC 5 cut(s) 666, 1388, 1663, 1745, 1874
BstF5I GGATG 5 cut(s) 76, 364, 1188, 1493, 1684
BstKTI GATC 7 cut(s) 259, 349, 739, 1048, 1189, 1404, 1455
BstMAI GTCTC 4 cut(s) 983, 1169, 1244, 1441
BstMBI GATC 7 cut(s) 256, 346, 736, 1045, 1186, 1401, 1452
BstMWI GCNNNNNNNGC 2 cut(s) 272, 1667
BstNI CCWGG 8 cut(s) 426, 457, 696, 768, 1133, 1375, 1407, 1485
BstNSI RCATGY 3 cut(s) 1295, 1665, 1876
BstSCI CCNGG 9 cut(s) 107, 424, 455, 694, 766, 1131, 1373, 1405, 1483
BstSLI GKGCMC 1 cut(s) 1495
BstV1I GCAGC 2 cut(s) 514, 1727
BstV2I GAAGAC 2 cut(s) 81, 230
BstX2I RGATCY 3 cut(s) 736, 1186, 1401
BstXI CCANNNNNNTGG 1 cut(s) 1310
BstYI RGATCY 3 cut(s) 736, 1186, 1401
Bsu15I ATCGAT 2 cut(s) 369, 645
Bsu36I CCTNAGG 1 cut(s) 1878
BsuRI GGCC 3 cut(s) 791, 1314, 1594
BsuTUI ATCGAT 2 cut(s) 369, 645
BtsCI GGATG 5 cut(s) 76, 364, 1188, 1493, 1684
BtsIMutI CAGTG 2 cut(s) 45, 68
BveI ACCTGC 1 cut(s) 1738
Cac8I GCNNGC 5 cut(s) 666, 1388, 1663, 1745, 1874
CaiI CAGNNNCTG 2 cut(s) 497, 767
CciI TCATGA 1 cut(s) 1645
Cfr10I RCCGGY 1 cut(s) 1514
Cfr13I GGNCC 3 cut(s) 467, 575, 790
ClaI ATCGAT 2 cut(s) 369, 645
CseI GACGC 2 cut(s) 531, 621
CsiI ACCWGGT 1 cut(s) 1373
Csp6I GTAC 7 cut(s) 93, 146, 1221, 1283, 1506, 1537, 1579
CspCI CAANNNNNGTGG 2 cut(s) 1379, 1414
CviQI GTAC 7 cut(s) 93, 146, 1221, 1283, 1506, 1537, 1579
DpnI GATC 7 cut(s) 258, 348, 738, 1047, 1188, 1403, 1454
DpnII GATC 7 cut(s) 256, 346, 736, 1045, 1186, 1401, 1452
DrdI GACNNNNNNGTC 1 cut(s) 83
DseDI GACNNNNNNGTC 1 cut(s) 83
Eam1104I CTCTTC 2 cut(s) 244, 1113
EarI CTCTTC 2 cut(s) 244, 1113
Eco130I CCWWGG 3 cut(s) 889, 1215, 1815
Eco24I GRGCYC 1 cut(s) 1470
Eco31I GGTCTC 2 cut(s) 1244, 1441
Eco32I GATATC 1 cut(s) 643
Eco47I GGWCC 2 cut(s) 467, 575
Eco57I CTGAAG 2 cut(s) 1409, 1707
Eco81I CCTNAGG 1 cut(s) 1878
EcoRI GAATTC 2 cut(s) 1110, 1759
EcoRII CCWGG 8 cut(s) 424, 455, 694, 766, 1131, 1373, 1405, 1483
EcoRV GATATC 1 cut(s) 643
EcoT14I CCWWGG 3 cut(s) 889, 1215, 1815
EcoT22I ATGCAT 1 cut(s) 592
EcoT38I GRGCYC 1 cut(s) 1470
ErhI CCWWGG 3 cut(s) 889, 1215, 1815
FaqI GGGAC 2 cut(s) 561, 947
FauI CCCGC 1 cut(s) 1296
FblI GTMKAC 1 cut(s) 1296
Fnu4HI GCNGC 4 cut(s) 11, 528, 1592, 1741
FokI GGATG 5 cut(s) 83, 371, 1195, 1500, 1691
FriOI GRGCYC 1 cut(s) 1470
Fsp4HI GCNGC 4 cut(s) 11, 528, 1592, 1741
FspBI CTAG 5 cut(s) 594, 665, 981, 1770, 1787
GluI GCNGC 4 cut(s) 11, 528, 1592, 1741
GsuI CTGGAG 1 cut(s) 1154
HaeIII GGCC 3 cut(s) 791, 1314, 1594
HapII CCGG 3 cut(s) 47, 109, 1515
HgaI GACGC 2 cut(s) 531, 621
Hin1I GRCGYC 1 cut(s) 81
HincII GTYRAC 3 cut(s) 205, 1416, 1800
HindII GTYRAC 3 cut(s) 205, 1416, 1800
HindIII AAGCTT 2 cut(s) 781, 849
HinfI GANTC 3 cut(s) 5, 112, 170
HpaII CCGG 3 cut(s) 47, 109, 1515
HphI GGTGA 2 cut(s) 1529, 1830
Hpy166II GTNNAC 6 cut(s) 205, 1297, 1416, 1508, 1579, 1800
Hpy188III TCNNGA 6 cut(s) 47, 88, 498, 1115, 1456, 1646
Hpy8I GTNNAC 6 cut(s) 205, 1297, 1416, 1508, 1579, 1800
Hpy99I CGWCG 2 cut(s) 83, 86
HpyAV CCTTC 5 cut(s) 127, 750, 1325, 1373, 1495
HpyCH4III ACNGT 5 cut(s) 184, 1039, 1823, 1849, 1920
HpyCH4IV ACGT 2 cut(s) 81, 1550
HpyF10VI GCNNNNNNNGC 2 cut(s) 272, 1667
HpySE526I ACGT 2 cut(s) 81, 1550
Hsp92I GRCGYC 1 cut(s) 81
Kpn2I TCCGGA 1 cut(s) 46
KpnI GGTACC 1 cut(s) 1224
Kzo9I GATC 7 cut(s) 256, 346, 736, 1045, 1186, 1401, 1452
LmnI GCTCC 3 cut(s) 272, 1211, 1682
Lsp1109I GCAGC 2 cut(s) 514, 1727
LweI GCATC 5 cut(s) 332, 577, 599, 1410, 1535
MabI ACCWGGT 1 cut(s) 1373
MaeI CTAG 5 cut(s) 594, 665, 981, 1770, 1787
MaeII ACGT 2 cut(s) 81, 1550
MaeIII GTNAC 3 cut(s) 543, 1268, 1885
MalI GATC 7 cut(s) 258, 348, 738, 1047, 1188, 1403, 1454
MboI GATC 7 cut(s) 256, 346, 736, 1045, 1186, 1401, 1452
MboII GAAGA 8 cut(s) 86, 179, 231, 235, 291, 1130, 1543, 1924
MflI RGATCY 3 cut(s) 736, 1186, 1401
MhlI GDGCHC 3 cut(s) 1470, 1495, 1614
MlyI GAGTC 2 cut(s) 14, 106
MmeI TCCRAC 2 cut(s) 895, 1189
MnlI CCTC 9 cut(s) 108, 130, 328, 702, 781, 1367, 1537, 1605, 1887
Mph1103I ATGCAT 1 cut(s) 592
MroI TCCGGA 1 cut(s) 46
MroXI GAANNNNTTC 2 cut(s) 505, 775
MseI TTAA 4 cut(s) 336, 474, 1553, 1924
MslI CAYNNNNRTG 2 cut(s) 1308, 1644
MspI CCGG 3 cut(s) 47, 109, 1515
MspR9I CCNGG 9 cut(s) 109, 426, 457, 696, 768, 1133, 1375, 1407, 1485
MvaI CCWGG 8 cut(s) 426, 457, 696, 768, 1133, 1375, 1407, 1485
MwoI GCNNNNNNNGC 2 cut(s) 272, 1667
NciI CCSGG 1 cut(s) 109
NdeII GATC 7 cut(s) 256, 346, 736, 1045, 1186, 1401, 1452
NheI GCTAGC 1 cut(s) 664
NlaIV GGNNCC 6 cut(s) 577, 1213, 1222, 1403, 1494, 1813
NmuCI GTSAC 2 cut(s) 543, 1268
NsiI ATGCAT 1 cut(s) 592
NspI RCATGY 3 cut(s) 1295, 1665, 1876
NspV TTCGAA 1 cut(s) 165
OliI CACNNNNGTG 1 cut(s) 1308
PaeI GCATGC 2 cut(s) 1665, 1876
PagI TCATGA 1 cut(s) 1645
PaqCI CACCTGC 1 cut(s) 1738
PdmI GAANNNNTTC 2 cut(s) 505, 775
PfeI GAWTC 1 cut(s) 170
PflFI GACNNNGTC 2 cut(s) 80, 83
PfoI TCCNGGA 5 cut(s) 107, 424, 694, 766, 1131
PkrI GCNGC 4 cut(s) 12, 529, 1593, 1742
PleI GAGTC 2 cut(s) 13, 106
PpsI GAGTC 2 cut(s) 13, 106
Psp6I CCWGG 8 cut(s) 424, 455, 694, 766, 1131, 1373, 1405, 1483
PspGI CCWGG 8 cut(s) 424, 455, 694, 766, 1131, 1373, 1405, 1483
PspN4I GGNNCC 6 cut(s) 577, 1213, 1222, 1403, 1494, 1813
PspPI GGNCC 3 cut(s) 467, 575, 790
PstNI CAGNNNCTG 2 cut(s) 497, 767
PsuI RGATCY 3 cut(s) 736, 1186, 1401
PsyI GACNNNGTC 2 cut(s) 80, 83
RsaI GTAC 7 cut(s) 94, 147, 1222, 1284, 1507, 1538, 1580
RsaNI GTAC 7 cut(s) 93, 146, 1221, 1283, 1506, 1537, 1579
RseI CAYNNNNRTG 2 cut(s) 1308, 1644
SaqAI TTAA 4 cut(s) 336, 474, 1553, 1924
SatI GCNGC 4 cut(s) 11, 528, 1592, 1741
Sau3AI GATC 7 cut(s) 256, 346, 736, 1045, 1186, 1401, 1452
Sau96I GGNCC 3 cut(s) 467, 575, 790
ScaI AGTACT 1 cut(s) 94
SchI GAGTC 2 cut(s) 14, 106
ScrFI CCNGG 9 cut(s) 109, 426, 457, 696, 768, 1133, 1375, 1407, 1485
SduI GDGCHC 3 cut(s) 1470, 1495, 1614
SexAI ACCWGGT 1 cut(s) 1373
SfaNI GCATC 5 cut(s) 332, 577, 599, 1410, 1535
SfuI TTCGAA 1 cut(s) 165
SinI GGWCC 2 cut(s) 467, 575
SmiMI CAYNNNNRTG 2 cut(s) 1308, 1644
SmlI CTYRAG 1 cut(s) 88
SmoI CTYRAG 1 cut(s) 88
SphI GCATGC 2 cut(s) 1665, 1876
SsiI CCGC 5 cut(s) 11, 413, 1289, 1591, 1633
SspI AATATT 2 cut(s) 907, 1158
SspMI CTAG 5 cut(s) 594, 665, 981, 1770, 1787
StyD4I CCNGG 9 cut(s) 107, 424, 455, 694, 766, 1131, 1373, 1405, 1483
StyI CCWWGG 3 cut(s) 889, 1215, 1815
TaaI ACNGT 5 cut(s) 184, 1039, 1823, 1849, 1920
TaiI ACGT 2 cut(s) 84, 1553
TaqI TCGA 7 cut(s) 165, 369, 375, 523, 645, 1318, 1529
TatI WGTACW 3 cut(s) 92, 1536, 1578
TauI GCSGC 2 cut(s) 13, 1594
TfiI GAWTC 1 cut(s) 170
Tru1I TTAA 4 cut(s) 336, 474, 1553, 1924
Tru9I TTAA 4 cut(s) 336, 474, 1553, 1924
TscAI CASTG 2 cut(s) 45, 68
TseFI GTSAC 2 cut(s) 543, 1268
TseI GCWGC 2 cut(s) 527, 1740
Tsp45I GTSAC 2 cut(s) 543, 1268
TspDTI ATGAA 6 cut(s) 87, 1011, 1248, 1514, 1601, 1893
TspRI CASTG 2 cut(s) 45, 68
Tth111I GACNNNGTC 2 cut(s) 80, 83
VpaK11BI GGWCC 2 cut(s) 467, 575
XapI RAATTY 5 cut(s) 1110, 1330, 1759, 1829, 1855
XceI RCATGY 3 cut(s) 1295, 1665, 1876
XcmI CCANNNNNNNNNTGG 1 cut(s) 453
XmiI GTMKAC 1 cut(s) 1296
XmnI GAANNNNTTC 2 cut(s) 505, 775
XspI CTAG 5 cut(s) 594, 665, 981, 1770, 1787
ZraI GACGTC 1 cut(s) 82
ZrmI AGTACT 1 cut(s) 94
Zsp2I ATGCAT 1 cut(s) 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.