Rroxscaffold_1G00046380

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003941
Physical Location & Seq
Reverse (-)
65545743 .. 65550188
4446 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00046380.1

Sequence Viewer

Length: 1482 bp
ATGCCACCTATCCCCTGGCTGTTGAATCCACCGAAGAGTATTGAAACTGCAGTTTCTGGAAGTAGTTCTTTTGATAGAAACTATCAGCAGGCTGCTAAGAGTTTTGCCTCAGCATCGCATAATGTGATAGACTTAGATGGTCCCAGTTGTGATGCATCTAGTTCCAATGGTGACTATACTGGTCTTTATTTGAACGAGTTTATAGATATCAATGAGTATGCCCAAATGATGGCTTATTTTGATGAAATGGATGTTCCTCCTGGAATAGAAGCACCTATCCCACATAGTCTCATGTTGTCAGATTTTAGAAGCAGTGTAAAATCAGTTTCTGGAAGTAGTTCAAGCTTGAAGATCCAAAAGGATAGTGTTAGTCACTCTCTTGCGATATCAGCATCTTGGAAACTTCCACAAGTTTCCAGCAGTAAAAAGAAACAATCTTATCTGCAACACCAAGGGAGTGCTTCTAGTCTCCCAGTGGGAGCAGAATCATCAGTATTTCAAACACAAACTCAAAACAATGTCTCTGTGAAGCCAGTTTCTAGCATGGATACAGAAAAAACTATGGGGAAGGTAAATGGCTTCAAACAATTTGATATAGTTGAAGATCATTCAGACCATCATTATACTAAGACGAGTCATTTAACAAAGCAGAAGAACTGGGCAAAGAAAATTCAGGAGGAGTGGAAGATCCTGGAGAATGATTTGCCTGATATGATATTCGTTAGAGTTTATGAGACAAGAATGGACCTCTTGAGAGCAGTAATTGTTGGAGCGGAGGGTACTCCTTACCATGATGGTCTCTTTTTCTTTGATATTTCCTTCCCCAGTGGTTACCCCAATGAGCCACCGCATGTCTACTACCATTCTGGTGGCCTTCGACTAAATCCAAATTTGTATGATTGTGGAAAAGTATGCCTCAGCCTTCTCAACACTTGGTCTGGGGGCAAGCAAGAGAAGTGGATCCCTGGTAAGTCAACGATGCTTCAGGTTTTGGTCTCTATACAAGGGCTGATCTTGAATACAGAGCCCTACTTCAATGAGCCTGGATGGGCACCTAAGAAGGGTACACCAAGCGGGAAAATGGCATCGAAGAAGTACAATGAGGACACGTTCATCCTATCTCTCAGGACGATGGTGTACATGATGAAGAAGCCTCCAAAGTGTTTTGAGGAGTTTGTTATCGGGCATTTCCGCAATCGTGCTCATGACATTCTAAGTGCATGTAAAGCATATATGGATGGAGCTCAAGTGGGGTGTCTAGTCAAAGGTCTGATTCAGGGAGTTAATGGTAACAAGAGCTGCTCACAGGCGTTTAAAAATTCTGTAGCTGGGTTTTTGCCTACTCTAGTCAAACAGTTTACACAGATTGGTGCCAAGGACTGTGAGAAATTTTTACCGCCAACAGCAGTCGGGAATAAGCAGAATGGTAGCATGCCTCAGGCTGCAAGACCAACGGTTTCAGAGTGGAAGAAACATTTTTAG

Protein Analysis

493

Amino Acids

54.62

Weight (kDa)

8.55

Isoelectric Point (pI)

42.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 224 - 342 2.4e-21 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1051, 1370
AccB7I CCANNNNNTGG 1 cut(s) 229
AccBSI CCGCTC 1 cut(s) 773
AccI GTMKAC 1 cut(s) 855
AciI CCGC 5 cut(s) 773, 848, 1074, 1192, 1397
AclWI GGATC 4 cut(s) 346, 682, 955, 968
AcsI RAATTY 4 cut(s) 669, 889, 1318, 1388
AcuI CTGAAG 1 cut(s) 968
AfaI GTAC 4 cut(s) 781, 1066, 1097, 1139
AfiI CCNNNNNNNGG 2 cut(s) 229, 1061
AflIII ACRYGT 1 cut(s) 1107
AjnI CCWGG 5 cut(s) 14, 259, 690, 964, 1042
AluBI AGCT 4 cut(s) 345, 1244, 1299, 1328
AluI AGCT 4 cut(s) 345, 1244, 1299, 1328
Alw21I GWGCWC 2 cut(s) 1204, 1246
Alw26I GTCTC 6 cut(s) 293, 473, 526, 728, 803, 1000
AlwI GGATC 4 cut(s) 346, 682, 955, 968
AlwNI CAGNNNCTG 2 cut(s) 56, 329
AoxI GGCC 1 cut(s) 871
ApeKI GCWGC 3 cut(s) 92, 1299, 1442
ApoI RAATTY 4 cut(s) 669, 889, 1318, 1388
Asp700I GAANNNNTTC 2 cut(s) 64, 337
AspS9I GGNCC 2 cut(s) 140, 745
AsuHPI GGTGA 1 cut(s) 182
AvaII GGWCC 2 cut(s) 140, 745
AxyI CCTNAGG 1 cut(s) 1437
BaeGI GKGCMC 1 cut(s) 1054
BamHI GGATCC 1 cut(s) 960
BanI GGYRCC 2 cut(s) 1051, 1370
BanII GRGCYC 2 cut(s) 1029, 1246
Bbv12I GWGCWC 2 cut(s) 1204, 1246
BbvCI CCTCAGC 2 cut(s) 109, 917
BbvI GCAGC 3 cut(s) 79, 1286, 1429
BccI CCATC 7 cut(s) 131, 223, 624, 788, 1041, 1126, 1232
BciT130I CCWGG 5 cut(s) 16, 261, 692, 966, 1044
BciVI GTATCC 1 cut(s) 541
BcoDI GTCTC 6 cut(s) 293, 473, 526, 728, 803, 1000
BfaI CTAG 5 cut(s) 159, 465, 540, 1259, 1346
BfmI CTRYAG 2 cut(s) 48, 1323
BfuI GTATCC 1 cut(s) 541
BisI GCNGC 3 cut(s) 93, 1300, 1443
BlsI GCNGC 3 cut(s) 94, 1301, 1444
Bme1390I CCNGG 5 cut(s) 16, 261, 692, 966, 1044
Bme18I GGWCC 2 cut(s) 140, 745
BmgT120I GGNCC 2 cut(s) 140, 745
BmiI GGNNCC 4 cut(s) 142, 962, 1053, 1372
BmrFI CCNGG 5 cut(s) 16, 261, 692, 966, 1044
BmrI ACTGGG 4 cut(s) 138, 467, 667, 819
BmsI GCATC 6 cut(s) 122, 142, 164, 401, 969, 1094
BmuI ACTGGG 4 cut(s) 138, 467, 667, 819
BpmI CTGGAG 1 cut(s) 713
Bpu10I CCTNAGC 2 cut(s) 109, 917
BpuEI CTTGAG 2 cut(s) 772, 1230
BsaI GGTCTC 2 cut(s) 803, 1000
BsaJI CCNNGG 4 cut(s) 14, 451, 964, 1374
BsaXI ACNNNNNCTCC 2 cut(s) 1233, 1263
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 1061
Bse1I ACTGG 6 cut(s) 144, 184, 473, 533, 662, 825
Bse21I CCTNAGG 1 cut(s) 1437
BseBI CCWGG 5 cut(s) 16, 261, 692, 966, 1044
BseDI CCNNGG 4 cut(s) 14, 451, 964, 1374
BseGI GGATG 4 cut(s) 256, 1052, 1113, 1243
BseLI CCNNNNNNNGG 2 cut(s) 229, 1061
BseMII CTCAG 4 cut(s) 123, 931, 1138, 1451
BseNI ACTGG 6 cut(s) 144, 184, 473, 533, 662, 825
BseRI GAGGAG 2 cut(s) 692, 1184
BseSI GKGCMC 1 cut(s) 1054
BseXI GCAGC 3 cut(s) 79, 1286, 1429
BseYI CCCAGC 1 cut(s) 1328
BshFI GGCC 1 cut(s) 873
BshNI GGYRCC 2 cut(s) 1051, 1370
BsiHKAI GWGCWC 2 cut(s) 1204, 1246
BslFI GGGAC 1 cut(s) 126
BslI CCNNNNNNNGG 2 cut(s) 229, 1061
BsmAI GTCTC 6 cut(s) 293, 473, 526, 728, 803, 1000
BsmFI GGGAC 1 cut(s) 126
BsnI GGCC 1 cut(s) 873
Bso31I GGTCTC 2 cut(s) 803, 1000
Bsp1286I GDGCHC 4 cut(s) 1029, 1054, 1204, 1246
Bsp1407I TGTACA 1 cut(s) 1137
Bsp143I GATC 5 cut(s) 351, 604, 687, 960, 1011
BspACI CCGC 5 cut(s) 773, 848, 1074, 1192, 1397
BspANI GGCC 1 cut(s) 873
BspCNI CTCAG 4 cut(s) 122, 930, 1137, 1450
BspHI TCATGA 1 cut(s) 1204
BspLI GGNNCC 4 cut(s) 142, 962, 1053, 1372
BspMAI CTGCAG 1 cut(s) 52
BspPI GGATC 4 cut(s) 346, 682, 955, 968
BspT107I GGYRCC 2 cut(s) 1051, 1370
BspTNI GGTCTC 2 cut(s) 803, 1000
BsrBI CCGCTC 1 cut(s) 773
BsrGI TGTACA 1 cut(s) 1137
BsrI ACTGG 6 cut(s) 144, 184, 473, 533, 662, 825
BssECI CCNNGG 4 cut(s) 14, 451, 964, 1374
BssMI GATC 5 cut(s) 351, 604, 687, 960, 1011
BssT1I CCWWGG 2 cut(s) 451, 1374
Bst2UI CCWGG 5 cut(s) 16, 261, 692, 966, 1044
Bst4CI ACNGT 3 cut(s) 1356, 1382, 1456
Bst6I CTCTTC 1 cut(s) 29
BstAUI TGTACA 1 cut(s) 1137
BstC8I GCNNGC 3 cut(s) 90, 947, 1433
BstDEI CTNAG 9 cut(s) 96, 109, 133, 627, 917, 1056, 1124, 1214, 1437
BstEII GGTNACC 1 cut(s) 830
BstF5I GGATG 4 cut(s) 256, 1052, 1113, 1243
BstKTI GATC 5 cut(s) 354, 607, 690, 963, 1014
BstMAI GTCTC 6 cut(s) 293, 473, 526, 728, 803, 1000
BstMBI GATC 5 cut(s) 351, 604, 687, 960, 1011
BstMWI GCNNNNNNNGC 2 cut(s) 389, 1226
BstNI CCWGG 5 cut(s) 16, 261, 692, 966, 1044
BstNSI RCATGY 3 cut(s) 854, 1224, 1435
BstPI GGTNACC 1 cut(s) 830
BstSCI CCNGG 5 cut(s) 14, 259, 690, 964, 1042
BstSFI CTRYAG 2 cut(s) 48, 1323
BstSLI GKGCMC 1 cut(s) 1054
BstV1I GCAGC 3 cut(s) 79, 1286, 1429
BstX2I RGATCY 3 cut(s) 351, 687, 960
BstXI CCANNNNNNTGG 1 cut(s) 869
BstYI RGATCY 3 cut(s) 351, 687, 960
Bsu36I CCTNAGG 1 cut(s) 1437
BsuI GTATCC 1 cut(s) 541
BsuRI GGCC 1 cut(s) 873
BtgZI GCGATG 1 cut(s) 99
BtsCI GGATG 4 cut(s) 256, 1052, 1113, 1243
BtsI GCAGTG 1 cut(s) 319
BtsIMutI CAGTG 3 cut(s) 319, 480, 832
Cac8I GCNNGC 3 cut(s) 90, 947, 1433
CaiI CAGNNNCTG 2 cut(s) 56, 329
CciI TCATGA 1 cut(s) 1204
Cfr13I GGNCC 2 cut(s) 140, 745
Csp6I GTAC 4 cut(s) 780, 1065, 1096, 1138
CspCI CAANNNNNGTGG 2 cut(s) 938, 973
CviAII CATG 8 cut(s) 292, 544, 791, 851, 1141, 1205, 1221, 1432
CviQI GTAC 4 cut(s) 780, 1065, 1096, 1138
DdeI CTNAG 9 cut(s) 96, 109, 133, 627, 917, 1056, 1124, 1214, 1437
DpnI GATC 5 cut(s) 353, 606, 689, 962, 1013
DpnII GATC 5 cut(s) 351, 604, 687, 960, 1011
DraI TTTAAA 1 cut(s) 1315
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Ecl136II GAGCTC 1 cut(s) 1244
Eco130I CCWWGG 2 cut(s) 451, 1374
Eco24I GRGCYC 2 cut(s) 1029, 1246
Eco31I GGTCTC 2 cut(s) 803, 1000
Eco32I GATATC 2 cut(s) 208, 387
Eco47I GGWCC 2 cut(s) 140, 745
Eco53kI GAGCTC 1 cut(s) 1244
Eco57I CTGAAG 1 cut(s) 968
Eco81I CCTNAGG 1 cut(s) 1437
Eco91I GGTNACC 1 cut(s) 830
EcoICRI GAGCTC 1 cut(s) 1244
EcoO65I GGTNACC 1 cut(s) 830
EcoRII CCWGG 5 cut(s) 14, 259, 690, 964, 1042
EcoRV GATATC 2 cut(s) 208, 387
EcoT14I CCWWGG 2 cut(s) 451, 1374
EcoT22I ATGCAT 1 cut(s) 157
EcoT38I GRGCYC 2 cut(s) 1029, 1246
ErhI CCWWGG 2 cut(s) 451, 1374
FaeI CATG 8 cut(s) 295, 547, 794, 854, 1144, 1208, 1224, 1435
FalI AAGNNNNNCTT 2 cut(s) 52, 84
FaqI GGGAC 1 cut(s) 126
FatI CATG 8 cut(s) 291, 543, 790, 850, 1140, 1204, 1220, 1431
FauI CCCGC 1 cut(s) 1067
FblI GTMKAC 1 cut(s) 855
Fnu4HI GCNGC 3 cut(s) 93, 1300, 1443
FokI GGATG 4 cut(s) 263, 1059, 1100, 1250
FriOI GRGCYC 2 cut(s) 1029, 1246
Fsp4HI GCNGC 3 cut(s) 93, 1300, 1443
FspBI CTAG 5 cut(s) 159, 465, 540, 1259, 1346
GluI GCNGC 3 cut(s) 93, 1300, 1443
GsaI CCCAGC 1 cut(s) 1332
GsuI CTGGAG 1 cut(s) 713
HaeIII GGCC 1 cut(s) 873
Hin1II CATG 8 cut(s) 295, 547, 794, 854, 1144, 1208, 1224, 1435
HincII GTYRAC 1 cut(s) 975
HindII GTYRAC 1 cut(s) 975
HindIII AAGCTT 1 cut(s) 343
HinfI GANTC 4 cut(s) 25, 485, 634, 1273
HphI GGTGA 1 cut(s) 182
Hpy166II GTNNAC 5 cut(s) 856, 975, 1067, 1138, 1359
Hpy188I TCNGA 4 cut(s) 301, 613, 1272, 1462
Hpy188III TCNNGA 8 cut(s) 57, 330, 674, 751, 1015, 1126, 1205, 1411
Hpy8I GTNNAC 5 cut(s) 856, 975, 1067, 1138, 1359
HpyAV CCTTC 5 cut(s) 562, 829, 884, 932, 1054
HpyCH4III ACNGT 3 cut(s) 1356, 1382, 1456
HpyCH4IV ACGT 1 cut(s) 1109
HpyCH4V TGCA 5 cut(s) 50, 155, 445, 1220, 1445
HpyF10VI GCNNNNNNNGC 2 cut(s) 389, 1226
HpyF3I CTNAG 9 cut(s) 96, 109, 133, 627, 917, 1056, 1124, 1214, 1437
HpySE526I ACGT 1 cut(s) 1109
Hsp92II CATG 8 cut(s) 295, 547, 794, 854, 1144, 1208, 1224, 1435
Kzo9I GATC 5 cut(s) 351, 604, 687, 960, 1011
LmnI GCTCC 3 cut(s) 479, 770, 1241
Lsp1109I GCAGC 3 cut(s) 79, 1286, 1429
LweI GCATC 6 cut(s) 122, 142, 164, 401, 969, 1094
MaeI CTAG 5 cut(s) 159, 465, 540, 1259, 1346
MaeII ACGT 1 cut(s) 1109
MaeIII GTNAC 4 cut(s) 170, 371, 830, 1289
MalI GATC 5 cut(s) 353, 606, 689, 962, 1013
MbiI CCGCTC 1 cut(s) 773
MboI GATC 5 cut(s) 351, 604, 687, 960, 1011
MboII GAAGA 8 cut(s) 46, 361, 614, 664, 697, 1102, 1159, 1480
MflI RGATCY 3 cut(s) 351, 687, 960
MhlI GDGCHC 4 cut(s) 1029, 1054, 1204, 1246
MluCI AATT 6 cut(s) 587, 669, 762, 889, 1318, 1388
MlyI GAGTC 1 cut(s) 643
MmeI TCCRAC 1 cut(s) 748
Mph1103I ATGCAT 1 cut(s) 157
MroXI GAANNNNTTC 2 cut(s) 64, 337
MseI TTAA 3 cut(s) 641, 1284, 1314
MslI CAYNNNNRTG 1 cut(s) 867
MspR9I CCNGG 5 cut(s) 16, 261, 692, 966, 1044
MvaI CCWGG 5 cut(s) 16, 261, 692, 966, 1044
MwoI GCNNNNNNNGC 2 cut(s) 389, 1226
NdeII GATC 5 cut(s) 351, 604, 687, 960, 1011
NlaIII CATG 8 cut(s) 295, 547, 794, 854, 1144, 1208, 1224, 1435
NlaIV GGNNCC 4 cut(s) 142, 962, 1053, 1372
NmuCI GTSAC 2 cut(s) 170, 371
NsiI ATGCAT 1 cut(s) 157
NspI RCATGY 3 cut(s) 854, 1224, 1435
PaeI GCATGC 1 cut(s) 1435
PagI TCATGA 1 cut(s) 1204
PdmI GAANNNNTTC 2 cut(s) 64, 337
PfeI GAWTC 3 cut(s) 25, 485, 1273
PflMI CCANNNNNTGG 1 cut(s) 229
PfoI TCCNGGA 2 cut(s) 259, 690
PkrI GCNGC 3 cut(s) 94, 1301, 1444
PleI GAGTC 1 cut(s) 642
PpsI GAGTC 1 cut(s) 642
Psp124BI GAGCTC 1 cut(s) 1246
Psp6I CCWGG 5 cut(s) 14, 259, 690, 964, 1042
PspEI GGTNACC 1 cut(s) 830
PspFI CCCAGC 1 cut(s) 1328
PspGI CCWGG 5 cut(s) 14, 259, 690, 964, 1042
PspN4I GGNNCC 4 cut(s) 142, 962, 1053, 1372
PspPI GGNCC 2 cut(s) 140, 745
PstI CTGCAG 1 cut(s) 52
PstNI CAGNNNCTG 2 cut(s) 56, 329
PsuI RGATCY 3 cut(s) 351, 687, 960
RsaI GTAC 4 cut(s) 781, 1066, 1097, 1139
RsaNI GTAC 4 cut(s) 780, 1065, 1096, 1138
RseI CAYNNNNRTG 1 cut(s) 867
SacI GAGCTC 1 cut(s) 1246
SaqAI TTAA 3 cut(s) 641, 1284, 1314
SatI GCNGC 3 cut(s) 93, 1300, 1443
Sau3AI GATC 5 cut(s) 351, 604, 687, 960, 1011
Sau96I GGNCC 2 cut(s) 140, 745
SchI GAGTC 1 cut(s) 643
ScrFI CCNGG 5 cut(s) 16, 261, 692, 966, 1044
SduI GDGCHC 4 cut(s) 1029, 1054, 1204, 1246
SfaNI GCATC 6 cut(s) 122, 142, 164, 401, 969, 1094
SfcI CTRYAG 2 cut(s) 48, 1323
SinI GGWCC 2 cut(s) 140, 745
SmiMI CAYNNNNRTG 1 cut(s) 867
SmlI CTYRAG 2 cut(s) 751, 1245
SmoI CTYRAG 2 cut(s) 751, 1245
SphI GCATGC 1 cut(s) 1435
Sse9I AATT 6 cut(s) 587, 669, 762, 889, 1318, 1388
SsiI CCGC 5 cut(s) 773, 848, 1074, 1192, 1397
SspMI CTAG 5 cut(s) 159, 465, 540, 1259, 1346
SstI GAGCTC 1 cut(s) 1246
StyD4I CCNGG 5 cut(s) 14, 259, 690, 964, 1042
StyI CCWWGG 2 cut(s) 451, 1374
TaaI ACNGT 3 cut(s) 1356, 1382, 1456
TaiI ACGT 1 cut(s) 1112
TaqI TCGA 2 cut(s) 877, 1088
TasI AATT 6 cut(s) 587, 669, 762, 889, 1318, 1388
TatI WGTACW 2 cut(s) 1095, 1137
TfiI GAWTC 3 cut(s) 25, 485, 1273
Tru1I TTAA 3 cut(s) 641, 1284, 1314
Tru9I TTAA 3 cut(s) 641, 1284, 1314
TscAI CASTG 3 cut(s) 319, 480, 832
TseFI GTSAC 2 cut(s) 170, 371
TseI GCWGC 3 cut(s) 92, 1299, 1442
Tsp45I GTSAC 2 cut(s) 170, 371
TspDTI ATGAA 3 cut(s) 258, 1102, 1160
TspRI CASTG 3 cut(s) 319, 480, 832
Van91I CCANNNNNTGG 1 cut(s) 229
VpaK11BI GGWCC 2 cut(s) 140, 745
XapI RAATTY 4 cut(s) 669, 889, 1318, 1388
XceI RCATGY 3 cut(s) 854, 1224, 1435
XcmI CCANNNNNNNNNTGG 1 cut(s) 12
XmiI GTMKAC 1 cut(s) 855
XmnI GAANNNNTTC 2 cut(s) 64, 337
XspI CTAG 5 cut(s) 159, 465, 540, 1259, 1346
Zsp2I ATGCAT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.