FvH4_3g20270

Translation initiation factor IF-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012929
Physical Location & Seq
Reverse (-)
13321695 .. 13323630
1936 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g20270.t1

Sequence Viewer

Length: 510 bp
ATGTTAACCTCCACATCATCACCTTCCTCCTCACTCCACTACCCAATCCTCCACTCCAAAACCCCAACCCTCAAATTCCAACCAAACTATCTCAAACTCCGACCAAACCCAAAACCTCAACTCCACCTCGGAGCCTCGGTCCTCACCTCCAAACGTTGTCCCAACATCGTTGCCATGAGCAAGTCCCCCAAAGTGGAGGAGCAGAAGTTCAGCGCCGAGGGTCTTGTCACTGAGTCTCTCCCGAATGGTATGTTTCGAATCCGATTAGACAACGCGGACAACATCATAGGATACATTTCTGGGAAAATCCGAAAGAGTTTCATTAGGATATTGCCTGGGGATAGAGTCAAAGTTGAGAAATTGCAGATAAACTTCGGCGACAACATACTTGCTGCGTTAAGTACCTTACTAGTACTTCCACAATCAAGAACAAAATTTCAAGCACATTGCAATTTAAAATGGAACGAAGCTACAAAAACCAAGAAGTTTGGATTTTACCACGAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0009507 GO:0009536 GO:0043021 GO:0043022 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0044877
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

170

Amino Acids

19.02

Weight (kDa)

10.05

Isoelectric Point (pI)

52.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-1a PF01176 73 - 120 2e-14 Translation initiation factor 1A / IF-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012929)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G11175
fragaria_vesca FvH4_3g20270
malus_domestica MD11G1244000.v1.1
prunus_persica Prupe.4G182300_v2.0.a1 Prupe.4G182400_v2.0.a1
pyrus_communis pycom03g17460 pycom11g21650
rosa_chinensis RchiOBHm_Chr5g0034161 RchiOBHm_CPg0501881
rosa_laevigata RLG00000033528
rosa_multiflora Rmu_ssc0000128.1_g000028
rosa_roxburghii Rroxscaffold_1G00046120
rosa_rugosa Rorug05G0144300
rosa_samantha Rh5AG235700 Rh5BG236500 Rh5CG266500 Rh5DG243500
rosa_wichuraiana Rw5G021360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 275
AciI CCGC 1 cut(s) 275
AclI AACGTT 1 cut(s) 154
AcsI RAATTY 2 cut(s) 74, 434
AfaI GTAC 2 cut(s) 403, 414
AfiI CCNNNNNNNGG 1 cut(s) 193
AgsI TTSAA 1 cut(s) 440
AhlI ACTAGT 1 cut(s) 409
AjnI CCWGG 1 cut(s) 334
AloI GAACNNNNNNTCC 2 cut(s) 191, 223
AluBI AGCT 1 cut(s) 470
AluI AGCT 1 cut(s) 470
Alw26I GTCTC 1 cut(s) 240
ApeKI GCWGC 1 cut(s) 392
ApoI RAATTY 2 cut(s) 74, 434
AspLEI GCGC 1 cut(s) 215
AspS9I GGNCC 1 cut(s) 139
AsuHPI GGTGA 2 cut(s) 12, 136
AsuII TTCGAA 1 cut(s) 256
AvaII GGWCC 1 cut(s) 139
BbvI GCAGC 1 cut(s) 379
BciT130I CCWGG 1 cut(s) 336
BciVI GTATCC 1 cut(s) 284
BcoDI GTCTC 1 cut(s) 240
BcuI ACTAGT 1 cut(s) 409
BfaI CTAG 1 cut(s) 410
BfoI RGCGCY 1 cut(s) 216
BfuI GTATCC 1 cut(s) 284
BisI GCNGC 1 cut(s) 393
BlsI GCNGC 1 cut(s) 394
BmcAI AGTACT 1 cut(s) 414
Bme1390I CCNGG 1 cut(s) 336
Bme18I GGWCC 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 139
BmiI GGNNCC 1 cut(s) 133
BmrFI CCNGG 1 cut(s) 336
Bpu14I TTCGAA 1 cut(s) 256
BsaJI CCNNGG 4 cut(s) 127, 135, 216, 335
BsaXI ACNNNNNCTCC 6 cut(s) 105, 123, 135, 153, 191, 221
Bsc4I CCNNNNNNNGG 1 cut(s) 193
Bse3DI GCAATG 1 cut(s) 445
BseBI CCWGG 1 cut(s) 336
BseDI CCNNGG 4 cut(s) 127, 135, 216, 335
BseLI CCNNNNNNNGG 1 cut(s) 193
BseMI GCAATG 1 cut(s) 445
BseMII CTCAG 1 cut(s) 222
BseRI GAGGAG 2 cut(s) 19, 212
BseXI GCAGC 1 cut(s) 379
Bsh1236I CGCG 1 cut(s) 275
BslFI GGGAC 2 cut(s) 144, 169
BslI CCNNNNNNNGG 1 cut(s) 193
BsmAI GTCTC 1 cut(s) 240
BsmFI GGGAC 2 cut(s) 144, 169
Bsp119I TTCGAA 1 cut(s) 256
BspACI CCGC 1 cut(s) 275
BspCNI CTCAG 1 cut(s) 223
BspFNI CGCG 1 cut(s) 275
BspLI GGNNCC 1 cut(s) 133
BspT104I TTCGAA 1 cut(s) 256
BsrDI GCAATG 1 cut(s) 445
BssECI CCNNGG 4 cut(s) 127, 135, 216, 335
Bst2UI CCWGG 1 cut(s) 336
BstBI TTCGAA 1 cut(s) 256
BstDEI CTNAG 1 cut(s) 231
BstFNI CGCG 1 cut(s) 275
BstH2I RGCGCY 1 cut(s) 216
BstHHI GCGC 1 cut(s) 215
BstMAI GTCTC 1 cut(s) 240
BstNI CCWGG 1 cut(s) 336
BstSCI CCNGG 1 cut(s) 334
BstUI CGCG 1 cut(s) 275
BstV1I GCAGC 1 cut(s) 379
BsuI GTATCC 1 cut(s) 284
BtsIMutI CAGTG 1 cut(s) 228
CfoI GCGC 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 139
Csp6I GTAC 2 cut(s) 402, 413
CviAII CATG 1 cut(s) 175
CviJI RGCY 2 cut(s) 134, 470
CviKI_1 RGCY 2 cut(s) 134, 470
CviQI GTAC 2 cut(s) 402, 413
DdeI CTNAG 1 cut(s) 231
DraI TTTAAA 1 cut(s) 456
Eco47I GGWCC 1 cut(s) 139
EcoRII CCWGG 1 cut(s) 334
FaeI CATG 1 cut(s) 178
FaiI YATR 4 cut(s) 176, 251, 287, 386
FaqI GGGAC 2 cut(s) 144, 169
FatI CATG 1 cut(s) 174
Fnu4HI GCNGC 1 cut(s) 393
Fsp4HI GCNGC 1 cut(s) 393
FspBI CTAG 1 cut(s) 410
GlaI GCGC 1 cut(s) 214
GluI GCNGC 1 cut(s) 393
HaeII RGCGCY 1 cut(s) 216
HhaI GCGC 1 cut(s) 215
Hin1II CATG 1 cut(s) 178
Hin6I GCGC 1 cut(s) 213
HinP1I GCGC 1 cut(s) 213
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 3 cut(s) 233, 258, 345
HpaI GTTAAC 1 cut(s) 6
HphI GGTGA 2 cut(s) 12, 136
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 4 cut(s) 101, 131, 263, 311
Hpy188III TCNNGA 2 cut(s) 241, 426
Hpy8I GTNNAC 1 cut(s) 6
HpyAV CCTTC 1 cut(s) 33
HpyCH4IV ACGT 1 cut(s) 154
HpyCH4V TGCA 2 cut(s) 364, 450
HpyF3I CTNAG 1 cut(s) 231
HpySE526I ACGT 1 cut(s) 154
Hsp92II CATG 1 cut(s) 178
HspAI GCGC 1 cut(s) 213
KspAI GTTAAC 1 cut(s) 6
LmnI GCTCC 2 cut(s) 131, 199
LpnPI CCDG 3 cut(s) 285, 321, 348
Lsp1109I GCAGC 1 cut(s) 379
MaeI CTAG 1 cut(s) 410
MaeII ACGT 1 cut(s) 154
MaeIII GTNAC 1 cut(s) 226
MluCI AATT 4 cut(s) 74, 359, 434, 451
MlyI GAGTC 2 cut(s) 242, 354
MmeI TCCRAC 2 cut(s) 103, 124
MseI TTAA 3 cut(s) 5, 398, 455
MspR9I CCNGG 1 cut(s) 336
MvaI CCWGG 1 cut(s) 336
MvnI CGCG 1 cut(s) 275
NlaIII CATG 1 cut(s) 178
NlaIV GGNNCC 1 cut(s) 133
NmeAIII GCCGAG 1 cut(s) 241
NmuCI GTSAC 1 cut(s) 226
NspV TTCGAA 1 cut(s) 256
PfeI GAWTC 1 cut(s) 258
PkrI GCNGC 1 cut(s) 394
PleI GAGTC 2 cut(s) 241, 353
PpsI GAGTC 2 cut(s) 241, 353
Psp1406I AACGTT 1 cut(s) 154
Psp6I CCWGG 1 cut(s) 334
PspGI CCWGG 1 cut(s) 334
PspN4I GGNNCC 1 cut(s) 133
PspPI GGNCC 1 cut(s) 139
RsaI GTAC 2 cut(s) 403, 414
RsaNI GTAC 2 cut(s) 402, 413
SaqAI TTAA 3 cut(s) 5, 398, 455
SatI GCNGC 1 cut(s) 393
Sau96I GGNCC 1 cut(s) 139
ScaI AGTACT 1 cut(s) 414
SchI GAGTC 2 cut(s) 242, 354
ScrFI CCNGG 1 cut(s) 336
SetI ASST 8 cut(s) 11, 25, 118, 129, 149, 157, 407, 472
SfuI TTCGAA 1 cut(s) 256
SinI GGWCC 1 cut(s) 139
SpeI ACTAGT 1 cut(s) 409
Sse9I AATT 4 cut(s) 74, 359, 434, 451
SsiI CCGC 1 cut(s) 275
SspMI CTAG 1 cut(s) 410
StyD4I CCNGG 1 cut(s) 334
TaiI ACGT 1 cut(s) 157
TaqI TCGA 1 cut(s) 256
TaqII GACCGA 1 cut(s) 127
TasI AATT 4 cut(s) 74, 359, 434, 451
TatI WGTACW 1 cut(s) 412
TfiI GAWTC 1 cut(s) 258
Tru1I TTAA 3 cut(s) 5, 398, 455
Tru9I TTAA 3 cut(s) 5, 398, 455
TscAI CASTG 1 cut(s) 235
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 1 cut(s) 392
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 1 cut(s) 310
TspRI CASTG 1 cut(s) 235
VpaK11BI GGWCC 1 cut(s) 139
XapI RAATTY 2 cut(s) 74, 434
XspI CTAG 1 cut(s) 410
ZrmI AGTACT 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.