RLG00000033528

Translation initiation factor IF-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0012929
Physical Location & Seq
Reverse (-)
28512068 .. 28512927
860 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033528

Sequence Viewer

Length: 411 bp
ATGTTGACCTCAACATCATCCTCACTCCACCACCCAATCCTCCACACTCCCCTCCACTCCAAAACCCCAACCCTCACCCCACCCTCCCTCAAATTCCAACCAAACCCAAAACCTCAGCTTCACCTCGGAACCTCAATCCTCATCTCCAAACGTTGTCCCAACATCGTTGCCATGAGTAAATCCCCGAAAGTTGAGGAGCAGAAGTTCAGCGCAGAGGGTCTGGTCACGGAGTCGCTCCCGAATGGTATGTTTCGAATCCGATTAGACAACGCGGACAACATCATAGGATACATTTCTGGGAAAATCCGGAAGAGTTTCATTAGGATTTTGCCAGGGGATAGAGTCAAAGTTGAGGATAGACGAGGCAAGGGTAGTGATGCTACTGATGCTTTGAGTGGCGGTTTGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0009507 GO:0009536 GO:0043021 GO:0043022 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0044877
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

137

Amino Acids

14.86

Weight (kDa)

9.86

Isoelectric Point (pI)

43.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-1a PF01176 72 - 120 1.4e-14 Translation initiation factor 1A / IF-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012929)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G11175
fragaria_vesca FvH4_3g20270
malus_domestica MD11G1244000.v1.1
prunus_persica Prupe.4G182300_v2.0.a1 Prupe.4G182400_v2.0.a1
pyrus_communis pycom03g17460 pycom11g21650
rosa_chinensis RchiOBHm_Chr5g0034161 RchiOBHm_CPg0501881
rosa_laevigata RLG00000033528
rosa_multiflora Rmu_ssc0000128.1_g000028
rosa_roxburghii Rroxscaffold_1G00046120
rosa_rugosa Rorug05G0144300
rosa_samantha Rh5AG235700 Rh5BG236500 Rh5CG266500 Rh5DG243500
rosa_wichuraiana Rw5G021360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 272
AccIII TCCGGA 1 cut(s) 306
AciI CCGC 2 cut(s) 272, 399
AclI AACGTT 1 cut(s) 151
AcsI RAATTY 1 cut(s) 92
AjnI CCWGG 1 cut(s) 331
AloI GAACNNNNNNTCC 2 cut(s) 188, 220
AluBI AGCT 1 cut(s) 118
AluI AGCT 1 cut(s) 118
Aor13HI TCCGGA 1 cut(s) 306
ApoI RAATTY 1 cut(s) 92
Asp700I GAANNNNTTC 1 cut(s) 314
AspLEI GCGC 1 cut(s) 212
AsuHPI GGTGA 2 cut(s) 67, 113
AsuII TTCGAA 1 cut(s) 253
BbvCI CCTCAGC 1 cut(s) 114
BciT130I CCWGG 1 cut(s) 333
BciVI GTATCC 1 cut(s) 281
BfuI GTATCC 1 cut(s) 281
Bme1390I CCNGG 1 cut(s) 333
BmiI GGNNCC 1 cut(s) 130
BmrFI CCNGG 1 cut(s) 333
BmsI GCATC 2 cut(s) 367, 376
Bpu10I CCTNAGC 1 cut(s) 114
Bpu14I TTCGAA 1 cut(s) 253
BsaJI CCNNGG 2 cut(s) 124, 332
BsaWI WCCGGW 1 cut(s) 306
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
BseAI TCCGGA 1 cut(s) 306
BseBI CCWGG 1 cut(s) 333
BseDI CCNNGG 2 cut(s) 124, 332
BseGI GGATG 1 cut(s) 17
BseMII CTCAG 1 cut(s) 128
BseRI GAGGAG 1 cut(s) 209
Bsh1236I CGCG 1 cut(s) 272
BsiSI CCGG 1 cut(s) 307
BslFI GGGAC 1 cut(s) 141
BsmFI GGGAC 1 cut(s) 141
Bsp119I TTCGAA 1 cut(s) 253
Bsp13I TCCGGA 1 cut(s) 306
BspACI CCGC 2 cut(s) 272, 399
BspCNI CTCAG 1 cut(s) 127
BspEI TCCGGA 1 cut(s) 306
BspFNI CGCG 1 cut(s) 272
BspLI GGNNCC 1 cut(s) 130
BspT104I TTCGAA 1 cut(s) 253
BssECI CCNNGG 2 cut(s) 124, 332
Bst2UI CCWGG 1 cut(s) 333
Bst6I CTCTTC 1 cut(s) 305
BstBI TTCGAA 1 cut(s) 253
BstDEI CTNAG 1 cut(s) 114
BstF5I GGATG 1 cut(s) 17
BstFNI CGCG 1 cut(s) 272
BstHHI GCGC 1 cut(s) 212
BstMWI GCNNNNNNNGC 1 cut(s) 386
BstNI CCWGG 1 cut(s) 333
BstSCI CCNGG 1 cut(s) 331
BstUI CGCG 1 cut(s) 272
BsuI GTATCC 1 cut(s) 281
BtsCI GGATG 1 cut(s) 17
CfoI GCGC 1 cut(s) 212
CviAII CATG 1 cut(s) 172
CviJI RGCY 1 cut(s) 118
CviKI_1 RGCY 1 cut(s) 118
DdeI CTNAG 1 cut(s) 114
Eam1104I CTCTTC 1 cut(s) 305
EarI CTCTTC 1 cut(s) 305
EcoRII CCWGG 1 cut(s) 331
FaeI CATG 1 cut(s) 175
FaiI YATR 3 cut(s) 173, 248, 284
FaqI GGGAC 1 cut(s) 141
FatI CATG 1 cut(s) 171
FokI GGATG 1 cut(s) 4
GlaI GCGC 1 cut(s) 211
HapII CCGG 1 cut(s) 307
HhaI GCGC 1 cut(s) 212
Hin1II CATG 1 cut(s) 175
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 3 cut(s) 230, 255, 342
HpaII CCGG 1 cut(s) 307
HphI GGTGA 2 cut(s) 67, 113
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 2 cut(s) 128, 260
Hpy188III TCNNGA 2 cut(s) 238, 307
Hpy8I GTNNAC 1 cut(s) 6
HpyCH4IV ACGT 1 cut(s) 151
HpyF10VI GCNNNNNNNGC 1 cut(s) 386
HpyF3I CTNAG 1 cut(s) 114
HpySE526I ACGT 1 cut(s) 151
Hsp92II CATG 1 cut(s) 175
HspAI GCGC 1 cut(s) 210
Kpn2I TCCGGA 1 cut(s) 306
LmnI GCTCC 2 cut(s) 196, 240
LpnPI CCDG 5 cut(s) 206, 282, 318, 320, 345
LweI GCATC 2 cut(s) 367, 376
MaeII ACGT 1 cut(s) 151
MaeIII GTNAC 1 cut(s) 223
MboII GAAGA 1 cut(s) 322
MluCI AATT 1 cut(s) 92
MlyI GAGTC 2 cut(s) 239, 351
MmeI TCCRAC 1 cut(s) 121
MroI TCCGGA 1 cut(s) 306
MroXI GAANNNNTTC 1 cut(s) 314
MspI CCGG 1 cut(s) 307
MspR9I CCNGG 1 cut(s) 333
MvaI CCWGG 1 cut(s) 333
MvnI CGCG 1 cut(s) 272
MwoI GCNNNNNNNGC 1 cut(s) 386
NlaIII CATG 1 cut(s) 175
NlaIV GGNNCC 1 cut(s) 130
NmuCI GTSAC 1 cut(s) 223
NspV TTCGAA 1 cut(s) 253
PdmI GAANNNNTTC 1 cut(s) 314
PfeI GAWTC 1 cut(s) 255
PleI GAGTC 2 cut(s) 238, 350
PpsI GAGTC 2 cut(s) 238, 350
Psp1406I AACGTT 1 cut(s) 151
Psp6I CCWGG 1 cut(s) 331
PspGI CCWGG 1 cut(s) 331
PspN4I GGNNCC 1 cut(s) 130
SchI GAGTC 2 cut(s) 239, 351
ScrFI CCNGG 1 cut(s) 333
SetI ASST 6 cut(s) 11, 115, 120, 126, 134, 154
SfaNI GCATC 2 cut(s) 367, 376
SfuI TTCGAA 1 cut(s) 253
Sse9I AATT 1 cut(s) 92
SsiI CCGC 2 cut(s) 272, 399
StyD4I CCNGG 1 cut(s) 331
TaiI ACGT 1 cut(s) 154
TaqI TCGA 1 cut(s) 253
TasI AATT 1 cut(s) 92
TfiI GAWTC 1 cut(s) 255
TseFI GTSAC 1 cut(s) 223
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 1 cut(s) 307
TspGWI ACGGA 1 cut(s) 242
XapI RAATTY 1 cut(s) 92
XmnI GAANNNNTTC 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.