pycom03g17460

Translation initiation factor IF-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0012929
Physical Location & Seq
Reverse (-)
18602052 .. 18602750
699 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g17460.1

Sequence Viewer

Length: 699 bp
ATGGGACCCAAAATTATGAATTACATATTCACGCTCGTAAACAATATTTACTCAATACAATCCTCAAAAAGCCCAAAGCCAATCCCTTTACCTCCTCCCCTTCTCTGTCTCCACCCACATAACAGACACCAAAAGAGAACACCACCACCACCAAAGGCCATGTTAACCTCGCCATCATCCTCCCTCCGCCACCCAATCCTCCACCACACTCCCCCTCCCCACTCCAAAACCCTAACCCTCACCCCATCAACTCTCAATTTCCAACCAGTCCAAAAACCCCAGTTCCATCTCCACATCAACATCAACACCAGCAGGCACTGTCCGGTCGGAAACCACCTCCTCTTCAGCACCATACGGTTTTCGAACGTTGTCGCCGCGAGCAAATCGCCGGGAGTGGACGAACAGAAGTTCAGCCAAGAAGGGATGGTGACGGAGTCACTCCCCAACGGTATGTTTCGAATTCGATTAGACAACGGGGACACCATCATCGGCTACATTTCTGGGAAAATCCGAAAGAAATTCATTCGAATTTTGATCGGCGACAGAGTTAAGGTTGAAGTCAGTCGGTACGACATCACGAGAGGTCGTATTGTTTTCAGATATAAACAGGATGACGACTCAAGCAAAAGAAAGATGAAGAACTCCGAAAAGAAAAATCAATCCAGGAAGGATCCTATGGACTCTTCCTCCCTAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0009507 GO:0009536 GO:0043021 GO:0043022 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0044877
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

233

Amino Acids

26.37

Weight (kDa)

10.38

Isoelectric Point (pI)

55.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-1a PF01176 139 - 200 1.3e-21 Translation initiation factor 1A / IF-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012929)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G11175
fragaria_vesca FvH4_3g20270
malus_domestica MD11G1244000.v1.1
prunus_persica Prupe.4G182300_v2.0.a1 Prupe.4G182400_v2.0.a1
pyrus_communis pycom03g17460 pycom11g21650
rosa_chinensis RchiOBHm_Chr5g0034161 RchiOBHm_CPg0501881
rosa_laevigata RLG00000033528
rosa_multiflora Rmu_ssc0000128.1_g000028
rosa_roxburghii Rroxscaffold_1G00046120
rosa_rugosa Rorug05G0144300
rosa_samantha Rh5AG235700 Rh5BG236500 Rh5CG266500 Rh5DG243500
rosa_wichuraiana Rw5G021360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 377
AciI CCGC 2 cut(s) 187, 375
AclI AACGTT 1 cut(s) 366
AclWI GGATC 2 cut(s) 665, 678
AcsI RAATTY 3 cut(s) 459, 518, 528
AcuI CTGAAG 1 cut(s) 328
AfaI GTAC 1 cut(s) 569
AgsI TTSAA 1 cut(s) 557
AjnI CCWGG 1 cut(s) 662
Alw26I GTCTC 1 cut(s) 113
AlwI GGATC 2 cut(s) 665, 678
AlwNI CAGNNNCTG 1 cut(s) 318
AoxI GGCC 1 cut(s) 156
ApoI RAATTY 3 cut(s) 459, 518, 528
AspS9I GGNCC 1 cut(s) 5
AsuC2I CCSGG 1 cut(s) 390
AsuHPI GGTGA 2 cut(s) 232, 439
AsuII TTCGAA 3 cut(s) 362, 457, 526
AvaII GGWCC 1 cut(s) 5
BamHI GGATCC 1 cut(s) 670
BauI CACGAG 1 cut(s) 577
BccI CCATC 5 cut(s) 181, 253, 294, 418, 491
BciT130I CCWGG 1 cut(s) 664
BcnI CCSGG 1 cut(s) 390
BcoDI GTCTC 1 cut(s) 113
BisI GCNGC 1 cut(s) 375
BlsI GCNGC 1 cut(s) 376
Bme1390I CCNGG 2 cut(s) 390, 664
Bme18I GGWCC 1 cut(s) 5
BmgT120I GGNCC 1 cut(s) 5
BmiI GGNNCC 3 cut(s) 6, 7, 672
BmrFI CCNGG 2 cut(s) 390, 664
BmrI ACTGGG 1 cut(s) 274
BmuI ACTGGG 1 cut(s) 274
Bpu14I TTCGAA 3 cut(s) 362, 457, 526
BpuEI CTTGAG 1 cut(s) 604
BpuMI CCSGG 1 cut(s) 390
BsaWI WCCGGW 1 cut(s) 322
BsaXI ACNNNNNCTCC 3 cut(s) 199, 229, 671
Bse1I ACTGG 2 cut(s) 266, 280
BseBI CCWGG 1 cut(s) 664
BseGI GGATG 3 cut(s) 176, 429, 616
BseNI ACTGG 2 cut(s) 266, 280
BseRI GAGGAG 2 cut(s) 84, 329
Bsh1236I CGCG 1 cut(s) 377
Bsh1285I CGRYCG 1 cut(s) 327
BshFI GGCC 1 cut(s) 158
BsiEI CGRYCG 1 cut(s) 327
BsiSI CCGG 2 cut(s) 323, 389
BslFI GGGAC 2 cut(s) 18, 491
BsmAI GTCTC 1 cut(s) 113
BsmFI GGGAC 2 cut(s) 18, 491
BsnI GGCC 1 cut(s) 158
Bsp119I TTCGAA 3 cut(s) 362, 457, 526
Bsp143I GATC 2 cut(s) 534, 670
BspACI CCGC 2 cut(s) 187, 375
BspANI GGCC 1 cut(s) 158
BspFNI CGCG 1 cut(s) 377
BspLI GGNNCC 3 cut(s) 6, 7, 672
BspPI GGATC 2 cut(s) 665, 678
BspT104I TTCGAA 3 cut(s) 362, 457, 526
BsrI ACTGG 2 cut(s) 266, 280
BssMI GATC 2 cut(s) 534, 670
BssSI CACGAG 1 cut(s) 577
Bst2BI CACGAG 1 cut(s) 577
Bst2UI CCWGG 1 cut(s) 664
Bst4CI ACNGT 3 cut(s) 320, 357, 449
Bst6I CTCTTC 2 cut(s) 347, 688
BstBI TTCGAA 3 cut(s) 362, 457, 526
BstC8I GCNNGC 2 cut(s) 314, 379
BstF5I GGATG 3 cut(s) 176, 429, 616
BstFNI CGCG 1 cut(s) 377
BstKTI GATC 2 cut(s) 537, 673
BstMAI GTCTC 1 cut(s) 113
BstMBI GATC 2 cut(s) 534, 670
BstMCI CGRYCG 1 cut(s) 327
BstNI CCWGG 1 cut(s) 664
BstSCI CCNGG 2 cut(s) 388, 662
BstUI CGCG 1 cut(s) 377
BstX2I RGATCY 1 cut(s) 670
BstYI RGATCY 1 cut(s) 670
BsuRI GGCC 1 cut(s) 158
BtsCI GGATG 3 cut(s) 176, 429, 616
BtsIMutI CAGTG 1 cut(s) 316
Cac8I GCNNGC 2 cut(s) 314, 379
CaiI CAGNNNCTG 1 cut(s) 318
Cfr13I GGNCC 1 cut(s) 5
Csp6I GTAC 1 cut(s) 568
CviAII CATG 1 cut(s) 160
CviJI RGCY 5 cut(s) 72, 79, 158, 414, 492
CviKI_1 RGCY 5 cut(s) 72, 79, 158, 414, 492
CviQI GTAC 1 cut(s) 568
DpnI GATC 2 cut(s) 536, 672
DpnII GATC 2 cut(s) 534, 670
Eam1104I CTCTTC 2 cut(s) 347, 688
EarI CTCTTC 2 cut(s) 347, 688
EciI GGCGGA 1 cut(s) 176
Eco47I GGWCC 1 cut(s) 5
Eco57I CTGAAG 1 cut(s) 328
EcoO109I RGGNCCY 1 cut(s) 5
EcoRI GAATTC 1 cut(s) 459
EcoRII CCWGG 1 cut(s) 662
FaeI CATG 1 cut(s) 163
FaiI YATR 8 cut(s) 17, 26, 120, 161, 353, 452, 603, 677
FaqI GGGAC 2 cut(s) 18, 491
FatI CATG 1 cut(s) 159
Fnu4HI GCNGC 1 cut(s) 375
FokI GGATG 3 cut(s) 163, 436, 623
Fsp4HI GCNGC 1 cut(s) 375
GluI GCNGC 1 cut(s) 375
HaeIII GGCC 1 cut(s) 158
HapII CCGG 2 cut(s) 323, 389
Hin1II CATG 1 cut(s) 163
HincII GTYRAC 1 cut(s) 165
HindII GTYRAC 1 cut(s) 165
HinfI GANTC 3 cut(s) 434, 617, 680
HpaI GTTAAC 1 cut(s) 165
HpaII CCGG 2 cut(s) 323, 389
HphI GGTGA 2 cut(s) 232, 439
Hpy166II GTNNAC 3 cut(s) 40, 165, 397
Hpy188I TCNGA 4 cut(s) 329, 512, 599, 646
Hpy188III TCNNGA 1 cut(s) 577
Hpy8I GTNNAC 3 cut(s) 40, 165, 397
HpyAV CCTTC 3 cut(s) 110, 413, 661
HpyCH4III ACNGT 3 cut(s) 320, 357, 449
HpyCH4IV ACGT 1 cut(s) 366
HpySE526I ACGT 1 cut(s) 366
Hsp92II CATG 1 cut(s) 163
KflI GGGWCCC 1 cut(s) 5
KspAI GTTAAC 1 cut(s) 165
Kzo9I GATC 2 cut(s) 534, 670
MaeII ACGT 1 cut(s) 366
MaeIII GTNAC 2 cut(s) 427, 435
MalI GATC 2 cut(s) 536, 672
MboI GATC 2 cut(s) 534, 670
MboII GAAGA 3 cut(s) 334, 649, 675
MflI RGATCY 1 cut(s) 670
MluCI AATT 6 cut(s) 12, 19, 256, 459, 518, 528
MlyI GAGTC 3 cut(s) 443, 611, 674
MmeI TCCRAC 2 cut(s) 286, 307
MseI TTAA 2 cut(s) 164, 549
MspI CCGG 2 cut(s) 323, 389
MspR9I CCNGG 2 cut(s) 390, 664
MvaI CCWGG 1 cut(s) 664
MvnI CGCG 1 cut(s) 377
NciI CCSGG 1 cut(s) 390
NdeII GATC 2 cut(s) 534, 670
NlaIII CATG 1 cut(s) 163
NlaIV GGNNCC 3 cut(s) 6, 7, 672
NmuCI GTSAC 2 cut(s) 427, 435
NspV TTCGAA 3 cut(s) 362, 457, 526
PflFI GACNNNGTC 1 cut(s) 433
PfoI TCCNGGA 1 cut(s) 662
PkrI GCNGC 1 cut(s) 376
PleI GAGTC 3 cut(s) 442, 611, 674
PpsI GAGTC 3 cut(s) 442, 611, 674
PpuMI RGGWCCY 1 cut(s) 5
Psp1406I AACGTT 1 cut(s) 366
Psp5II RGGWCCY 1 cut(s) 5
Psp6I CCWGG 1 cut(s) 662
PspGI CCWGG 1 cut(s) 662
PspN4I GGNNCC 3 cut(s) 6, 7, 672
PspPI GGNCC 1 cut(s) 5
PspPPI RGGWCCY 1 cut(s) 5
PstNI CAGNNNCTG 1 cut(s) 318
PsuI RGATCY 1 cut(s) 670
PsyI GACNNNGTC 1 cut(s) 433
RsaI GTAC 1 cut(s) 569
RsaNI GTAC 1 cut(s) 568
SaqAI TTAA 2 cut(s) 164, 549
SatI GCNGC 1 cut(s) 375
Sau3AI GATC 2 cut(s) 534, 670
Sau96I GGNCC 1 cut(s) 5
SchI GAGTC 3 cut(s) 443, 611, 674
ScrFI CCNGG 2 cut(s) 390, 664
SetI ASST 6 cut(s) 94, 170, 339, 369, 555, 586
SfuI TTCGAA 3 cut(s) 362, 457, 526
SinI GGWCC 1 cut(s) 5
SmlI CTYRAG 1 cut(s) 619
SmoI CTYRAG 1 cut(s) 619
Sse9I AATT 6 cut(s) 12, 19, 256, 459, 518, 528
SsiI CCGC 2 cut(s) 187, 375
SspI AATATT 1 cut(s) 46
StyD4I CCNGG 2 cut(s) 388, 662
TaaI ACNGT 3 cut(s) 320, 357, 449
TaiI ACGT 1 cut(s) 369
TaqI TCGA 4 cut(s) 362, 457, 463, 526
TasI AATT 6 cut(s) 12, 19, 256, 459, 518, 528
TauI GCSGC 1 cut(s) 377
Tru1I TTAA 2 cut(s) 164, 549
Tru9I TTAA 2 cut(s) 164, 549
TscAI CASTG 1 cut(s) 323
TseFI GTSAC 2 cut(s) 427, 435
Tsp45I GTSAC 2 cut(s) 427, 435
TspDTI ATGAA 3 cut(s) 32, 511, 650
TspGWI ACGGA 1 cut(s) 446
TspRI CASTG 1 cut(s) 323
Tth111I GACNNNGTC 1 cut(s) 433
VpaK11BI GGWCC 1 cut(s) 5
XapI RAATTY 3 cut(s) 459, 518, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.