Rw5G021360

Translation initiation factor IF-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0012929
Physical Location & Seq
Forward (+)
28685289 .. 28686876
1588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G021360.1

Sequence Viewer

Length: 426 bp
ATGTTAACCTCAACATCATCATCCTCACTTCACCACCCAATCCTCCACACTCCCCTCCACTCCAAAACCCCAACCCTCACCCCACCCTCCCTCAAATTCCAACCAAACCCAAAACCTCAGCTTCACCTCGGAACCTCAATCCTCATCTCCAAACGTTGTCCCAACATCGTTGCCATGAGTAAATCCCCGAAAGTTGAGGAGCAGAAGTTCAGCGCAGAGGGTCTGGTCACTGAGTCACTCCCGAACGGCATGTTTCGAATCCGATTAGACAACGCGGACAACATCATAGGATACATTTCTGGGAAAATTCGAAAGAGTTTCATTAGGATTTTACCTGGGGATAGAGTCAAAGTTGAGGTCAGTCGCTATGATACTACTAGAGGTCGAATTGTTTATAGACTCCGTAACACTAGGGATTCTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0009507 GO:0009536 GO:0043021 GO:0043022 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0044877
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

141

Amino Acids

15.83

Weight (kDa)

10.47

Isoelectric Point (pI)

44.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-1a PF01176 73 - 133 1.6e-21 Translation initiation factor 1A / IF-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012929)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G11175
fragaria_vesca FvH4_3g20270
malus_domestica MD11G1244000.v1.1
prunus_persica Prupe.4G182300_v2.0.a1 Prupe.4G182400_v2.0.a1
pyrus_communis pycom03g17460 pycom11g21650
rosa_chinensis RchiOBHm_Chr5g0034161 RchiOBHm_CPg0501881
rosa_laevigata RLG00000033528
rosa_multiflora Rmu_ssc0000128.1_g000028
rosa_roxburghii Rroxscaffold_1G00046120
rosa_rugosa Rorug05G0144300
rosa_samantha Rh5AG235700 Rh5BG236500 Rh5CG266500 Rh5DG243500
rosa_wichuraiana Rw5G021360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 275
AciI CCGC 1 cut(s) 275
AclI AACGTT 1 cut(s) 154
AcsI RAATTY 2 cut(s) 95, 306
AjnI CCWGG 1 cut(s) 334
AloI GAACNNNNNNTCC 2 cut(s) 191, 223
AluBI AGCT 1 cut(s) 121
AluI AGCT 1 cut(s) 121
ApoI RAATTY 2 cut(s) 95, 306
ArsI GACNNNNNNTTYG 2 cut(s) 342, 374
AspLEI GCGC 1 cut(s) 215
AsuHPI GGTGA 3 cut(s) 23, 70, 116
AsuII TTCGAA 2 cut(s) 256, 310
BbvCI CCTCAGC 1 cut(s) 117
BceAI ACGGC 1 cut(s) 262
BciT130I CCWGG 1 cut(s) 336
BciVI GTATCC 1 cut(s) 284
BfaI CTAG 2 cut(s) 378, 411
BfuI GTATCC 1 cut(s) 284
Bme1390I CCNGG 1 cut(s) 336
BmiI GGNNCC 1 cut(s) 133
BmrFI CCNGG 1 cut(s) 336
Bpu10I CCTNAGC 1 cut(s) 117
Bpu14I TTCGAA 2 cut(s) 256, 310
BsaJI CCNNGG 2 cut(s) 127, 335
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
BseBI CCWGG 1 cut(s) 336
BseDI CCNNGG 2 cut(s) 127, 335
BseGI GGATG 1 cut(s) 20
BseMII CTCAG 2 cut(s) 131, 222
BseRI GAGGAG 1 cut(s) 212
Bsh1236I CGCG 1 cut(s) 275
BslFI GGGAC 1 cut(s) 144
BsmFI GGGAC 1 cut(s) 144
Bsp119I TTCGAA 2 cut(s) 256, 310
BspACI CCGC 1 cut(s) 275
BspCNI CTCAG 2 cut(s) 130, 223
BspFNI CGCG 1 cut(s) 275
BspLI GGNNCC 1 cut(s) 133
BspT104I TTCGAA 2 cut(s) 256, 310
BssECI CCNNGG 2 cut(s) 127, 335
Bst2UI CCWGG 1 cut(s) 336
BstBI TTCGAA 2 cut(s) 256, 310
BstDEI CTNAG 2 cut(s) 117, 231
BstF5I GGATG 1 cut(s) 20
BstFNI CGCG 1 cut(s) 275
BstHHI GCGC 1 cut(s) 215
BstNI CCWGG 1 cut(s) 336
BstNSI RCATGY 1 cut(s) 253
BstSCI CCNGG 1 cut(s) 334
BstUI CGCG 1 cut(s) 275
BsuI GTATCC 1 cut(s) 284
BtsCI GGATG 1 cut(s) 20
BtsIMutI CAGTG 1 cut(s) 228
CfoI GCGC 1 cut(s) 215
CviAII CATG 2 cut(s) 175, 250
CviJI RGCY 1 cut(s) 121
CviKI_1 RGCY 1 cut(s) 121
DdeI CTNAG 2 cut(s) 117, 231
EcoRII CCWGG 1 cut(s) 334
FaeI CATG 2 cut(s) 178, 253
FaiI YATR 5 cut(s) 176, 251, 287, 369, 396
FaqI GGGAC 1 cut(s) 144
FatI CATG 2 cut(s) 174, 249
FokI GGATG 1 cut(s) 7
FspBI CTAG 2 cut(s) 378, 411
GlaI GCGC 1 cut(s) 214
HhaI GCGC 1 cut(s) 215
Hin1II CATG 2 cut(s) 178, 253
Hin6I GCGC 1 cut(s) 213
HinP1I GCGC 1 cut(s) 213
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 5 cut(s) 233, 258, 345, 399, 416
HpaI GTTAAC 1 cut(s) 6
HphI GGTGA 3 cut(s) 23, 70, 116
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 2 cut(s) 131, 263
Hpy188III TCNNGA 1 cut(s) 241
Hpy8I GTNNAC 1 cut(s) 6
HpyCH4IV ACGT 1 cut(s) 154
HpyF3I CTNAG 2 cut(s) 117, 231
HpySE526I ACGT 1 cut(s) 154
Hsp92II CATG 2 cut(s) 178, 253
HspAI GCGC 1 cut(s) 213
KspAI GTTAAC 1 cut(s) 6
LmnI GCTCC 1 cut(s) 199
LpnPI CCDG 4 cut(s) 209, 285, 321, 348
MaeI CTAG 2 cut(s) 378, 411
MaeII ACGT 1 cut(s) 154
MaeIII GTNAC 3 cut(s) 226, 234, 404
MluCI AATT 3 cut(s) 95, 306, 387
MlyI GAGTC 3 cut(s) 242, 354, 393
MmeI TCCRAC 1 cut(s) 124
MseI TTAA 1 cut(s) 5
MspR9I CCNGG 1 cut(s) 336
MvaI CCWGG 1 cut(s) 336
MvnI CGCG 1 cut(s) 275
NlaIII CATG 2 cut(s) 178, 253
NlaIV GGNNCC 1 cut(s) 133
NmuCI GTSAC 2 cut(s) 226, 234
NspI RCATGY 1 cut(s) 253
NspV TTCGAA 2 cut(s) 256, 310
PfeI GAWTC 2 cut(s) 258, 416
PleI GAGTC 3 cut(s) 241, 353, 393
PpsI GAGTC 3 cut(s) 241, 353, 393
Psp1406I AACGTT 1 cut(s) 154
Psp6I CCWGG 1 cut(s) 334
PspGI CCWGG 1 cut(s) 334
PspN4I GGNNCC 1 cut(s) 133
SaqAI TTAA 1 cut(s) 5
SchI GAGTC 3 cut(s) 242, 354, 393
ScrFI CCNGG 1 cut(s) 336
SetI ASST 9 cut(s) 11, 118, 123, 129, 137, 157, 337, 360, 385
SfuI TTCGAA 2 cut(s) 256, 310
Sse9I AATT 3 cut(s) 95, 306, 387
SsiI CCGC 1 cut(s) 275
SspMI CTAG 2 cut(s) 378, 411
StyD4I CCNGG 1 cut(s) 334
TaiI ACGT 1 cut(s) 157
TaqI TCGA 3 cut(s) 256, 310, 385
TasI AATT 3 cut(s) 95, 306, 387
TfiI GAWTC 2 cut(s) 258, 416
Tru1I TTAA 1 cut(s) 5
Tru9I TTAA 1 cut(s) 5
TscAI CASTG 1 cut(s) 235
TseFI GTSAC 2 cut(s) 226, 234
Tsp45I GTSAC 2 cut(s) 226, 234
TspDTI ATGAA 1 cut(s) 310
TspGWI ACGGA 1 cut(s) 392
TspRI CASTG 1 cut(s) 235
XapI RAATTY 2 cut(s) 95, 306
XceI RCATGY 1 cut(s) 253
XspI CTAG 2 cut(s) 378, 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.