pycom11g21650

Translation initiation factor IF-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0012929
Physical Location & Seq
Forward (+)
24723834 .. 24724568
735 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g21650.1

Sequence Viewer

Length: 735 bp
ATGAGAAAGAAGAGAAAAAGAATTCTAATGAAACTACATATTCACCCTCGACAGAATCCAATATTTACTCAAAAGTCAAAACAATCCTCAAAAATCCCCAAACCCAATCCCTTTACCTCCTCCCCTTTCCTGTCTCCACCCACAAAACAGACACCAAAAGCGAACACCGTTACCAGCACCACCACCAAAGCCATGTTAGCCTCGCCATCATCCTCCCTCCGCCACCCAATCGTCCACCACTCTCCCCCATTCCACTCTAAAACCCTAACCATAACCCTAACCCTCACCCCATCAACTCTCAAATTCCAACCAAACCCAAAACCCCAGTCCCATCTCCACATCACCAACAGCAGCAGCAGCCACTGTCTTGTCGGAAACCACTTCTTCCTCAGCCCCAAACGGTTCCCGTTCGTTGTCGCCGCGAGCAAATCCCCGGGAGTGGACGAGCAGAAGTTCAGCCAAGAAGGGTTGGTCACGGAGTCGCTCCCCAACGGTATGTTTCGAATCCGATTAGACAACGCCGACACTATCATCGGCTACATTTCTGGGAAAATCCGAAAGAAATTCATTCGAATTTTGATCGGAGACAGAGTTAAGGTTGAGGTCAGCCGGTACGACACCACTAGAGGTCGTATTGTTTTCAGGTATAAACAGGATGACGACCCAAGCAAAAGAAAGATGAAGAATTCCGAAACGAAAAATAAATCCAGGAAGGATCCTATGGACTCTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0009507 GO:0009536 GO:0043021 GO:0043022 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0044877
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

245

Amino Acids

27.68

Weight (kDa)

10.82

Isoelectric Point (pI)

57.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-1a PF01176 154 - 215 8.5e-21 Translation initiation factor 1A / IF-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012929)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G11175
fragaria_vesca FvH4_3g20270
malus_domestica MD11G1244000.v1.1
prunus_persica Prupe.4G182300_v2.0.a1 Prupe.4G182400_v2.0.a1
pyrus_communis pycom03g17460 pycom11g21650
rosa_chinensis RchiOBHm_Chr5g0034161 RchiOBHm_CPg0501881
rosa_laevigata RLG00000033528
rosa_multiflora Rmu_ssc0000128.1_g000028
rosa_roxburghii Rroxscaffold_1G00046120
rosa_rugosa Rorug05G0144300
rosa_samantha Rh5AG235700 Rh5BG236500 Rh5CG266500 Rh5DG243500
rosa_wichuraiana Rw5G021360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 422
AciI CCGC 2 cut(s) 220, 420
AclWI GGATC 2 cut(s) 710, 723
AcsI RAATTY 5 cut(s) 21, 302, 563, 573, 685
AfaI GTAC 1 cut(s) 614
AfiI CCNNNNNNNGG 1 cut(s) 439
AjnI CCWGG 1 cut(s) 707
Alw26I GTCTC 2 cut(s) 138, 579
AlwI GGATC 2 cut(s) 710, 723
AlwNI CAGNNNCTG 1 cut(s) 363
Ama87I CYCGRG 1 cut(s) 433
ApeKI GCWGC 3 cut(s) 351, 354, 357
ApoI RAATTY 5 cut(s) 21, 302, 563, 573, 685
ArsI GACNNNNNNTTYG 2 cut(s) 311, 343
AsuC2I CCSGG 2 cut(s) 434, 435
AsuHPI GGTGA 3 cut(s) 35, 277, 334
AsuII TTCGAA 2 cut(s) 502, 571
AvaI CYCGRG 1 cut(s) 433
BamHI GGATCC 1 cut(s) 715
BbvCI CCTCAGC 1 cut(s) 389
BbvI GCAGC 3 cut(s) 363, 366, 369
BccI CCATC 3 cut(s) 214, 298, 339
BciT130I CCWGG 1 cut(s) 709
BcnI CCSGG 2 cut(s) 434, 435
BcoDI GTCTC 2 cut(s) 138, 579
BfaI CTAG 1 cut(s) 624
BisI GCNGC 4 cut(s) 352, 355, 358, 420
BlsI GCNGC 4 cut(s) 353, 356, 359, 421
Bme1390I CCNGG 3 cut(s) 434, 435, 709
BmeT110I CYCGRG 1 cut(s) 433
BmiI GGNNCC 2 cut(s) 404, 717
BmrFI CCNGG 3 cut(s) 434, 435, 709
BmrI ACTGGG 1 cut(s) 319
BmuI ACTGGG 1 cut(s) 319
Bpu10I CCTNAGC 1 cut(s) 389
Bpu14I TTCGAA 2 cut(s) 502, 571
BpuMI CCSGG 2 cut(s) 434, 435
BsaJI CCNNGG 2 cut(s) 432, 433
BsaXI ACNNNNNCTCC 2 cut(s) 576, 606
Bsc4I CCNNNNNNNGG 1 cut(s) 439
Bse118I RCCGGY 1 cut(s) 609
Bse1I ACTGG 1 cut(s) 325
BseBI CCWGG 1 cut(s) 709
BseDI CCNNGG 2 cut(s) 432, 433
BseGI GGATG 2 cut(s) 209, 661
BseLI CCNNNNNNNGG 1 cut(s) 439
BseMII CTCAG 1 cut(s) 403
BseNI ACTGG 1 cut(s) 325
BseRI GAGGAG 1 cut(s) 109
BseXI GCAGC 3 cut(s) 363, 366, 369
Bsh1236I CGCG 1 cut(s) 422
BsiHKCI CYCGRG 1 cut(s) 433
BsiSI CCGG 2 cut(s) 434, 610
BslFI GGGAC 1 cut(s) 313
BslI CCNNNNNNNGG 1 cut(s) 439
BsmAI GTCTC 2 cut(s) 138, 579
BsmFI GGGAC 1 cut(s) 313
BsoBI CYCGRG 1 cut(s) 433
Bsp119I TTCGAA 2 cut(s) 502, 571
Bsp143I GATC 2 cut(s) 579, 715
BspACI CCGC 2 cut(s) 220, 420
BspCNI CTCAG 1 cut(s) 402
BspFNI CGCG 1 cut(s) 422
BspLI GGNNCC 2 cut(s) 404, 717
BspPI GGATC 2 cut(s) 710, 723
BspT104I TTCGAA 2 cut(s) 502, 571
BsrFI RCCGGY 1 cut(s) 609
BsrI ACTGG 1 cut(s) 325
BssAI RCCGGY 1 cut(s) 609
BssECI CCNNGG 2 cut(s) 432, 433
BssMI GATC 2 cut(s) 579, 715
Bst2UI CCWGG 1 cut(s) 709
Bst4CI ACNGT 4 cut(s) 169, 365, 402, 494
Bst6I CTCTTC 1 cut(s) 5
BstBI TTCGAA 2 cut(s) 502, 571
BstC8I GCNNGC 1 cut(s) 424
BstDEI CTNAG 1 cut(s) 389
BstF5I GGATG 2 cut(s) 209, 661
BstFNI CGCG 1 cut(s) 422
BstKTI GATC 2 cut(s) 582, 718
BstMAI GTCTC 2 cut(s) 138, 579
BstMBI GATC 2 cut(s) 579, 715
BstMWI GCNNNNNNNGC 2 cut(s) 197, 357
BstNI CCWGG 1 cut(s) 709
BstSCI CCNGG 3 cut(s) 432, 433, 707
BstUI CGCG 1 cut(s) 422
BstV1I GCAGC 3 cut(s) 363, 366, 369
BstX2I RGATCY 1 cut(s) 715
BstYI RGATCY 1 cut(s) 715
BtsCI GGATG 2 cut(s) 209, 661
BtsIMutI CAGTG 1 cut(s) 361
Cac8I GCNNGC 1 cut(s) 424
CaiI CAGNNNCTG 1 cut(s) 363
Cfr10I RCCGGY 1 cut(s) 609
Cfr9I CCCGGG 1 cut(s) 433
Csp6I GTAC 1 cut(s) 613
CviAII CATG 1 cut(s) 193
CviJI RGCY 7 cut(s) 191, 200, 360, 393, 459, 537, 609
CviKI_1 RGCY 7 cut(s) 191, 200, 360, 393, 459, 537, 609
CviQI GTAC 1 cut(s) 613
DdeI CTNAG 1 cut(s) 389
DpnI GATC 2 cut(s) 581, 717
DpnII GATC 2 cut(s) 579, 715
Eam1104I CTCTTC 1 cut(s) 5
EarI CTCTTC 1 cut(s) 5
EciI GGCGGA 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 433
EcoRI GAATTC 2 cut(s) 21, 685
EcoRII CCWGG 1 cut(s) 707
FaeI CATG 1 cut(s) 196
FaiI YATR 6 cut(s) 39, 194, 272, 497, 648, 722
FaqI GGGAC 1 cut(s) 313
FatI CATG 1 cut(s) 192
Fnu4HI GCNGC 4 cut(s) 352, 355, 358, 420
FokI GGATG 2 cut(s) 196, 668
Fsp4HI GCNGC 4 cut(s) 352, 355, 358, 420
FspBI CTAG 1 cut(s) 624
GluI GCNGC 4 cut(s) 352, 355, 358, 420
HapII CCGG 2 cut(s) 434, 610
Hin1II CATG 1 cut(s) 196
HinfI GANTC 4 cut(s) 55, 479, 504, 725
HpaII CCGG 2 cut(s) 434, 610
HphI GGTGA 3 cut(s) 35, 277, 334
Hpy166II GTNNAC 2 cut(s) 235, 442
Hpy188I TCNGA 5 cut(s) 374, 509, 557, 584, 691
Hpy8I GTNNAC 2 cut(s) 235, 442
HpyAV CCTTC 2 cut(s) 458, 706
HpyCH4III ACNGT 4 cut(s) 169, 365, 402, 494
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 357
HpyF3I CTNAG 1 cut(s) 389
Hsp92II CATG 1 cut(s) 196
Kzo9I GATC 2 cut(s) 579, 715
LmnI GCTCC 1 cut(s) 489
Lsp1109I GCAGC 3 cut(s) 363, 366, 369
MaeI CTAG 1 cut(s) 624
MaeIII GTNAC 2 cut(s) 169, 472
MalI GATC 2 cut(s) 581, 717
MboI GATC 2 cut(s) 579, 715
MboII GAAGA 3 cut(s) 22, 376, 694
MflI RGATCY 1 cut(s) 715
MluCI AATT 5 cut(s) 21, 302, 563, 573, 685
MlyI GAGTC 2 cut(s) 488, 719
MmeI TCCRAC 2 cut(s) 331, 352
MseI TTAA 1 cut(s) 594
MspI CCGG 2 cut(s) 434, 610
MspR9I CCNGG 3 cut(s) 434, 435, 709
MvaI CCWGG 1 cut(s) 709
MvnI CGCG 1 cut(s) 422
MwoI GCNNNNNNNGC 2 cut(s) 197, 357
NciI CCSGG 2 cut(s) 434, 435
NdeII GATC 2 cut(s) 579, 715
NlaIII CATG 1 cut(s) 196
NlaIV GGNNCC 2 cut(s) 404, 717
NmuCI GTSAC 1 cut(s) 472
NspV TTCGAA 2 cut(s) 502, 571
PfeI GAWTC 2 cut(s) 55, 504
PfoI TCCNGGA 1 cut(s) 707
PkrI GCNGC 4 cut(s) 353, 356, 359, 421
PleI GAGTC 2 cut(s) 487, 719
PpsI GAGTC 2 cut(s) 487, 719
Psp6I CCWGG 1 cut(s) 707
PspGI CCWGG 1 cut(s) 707
PspN4I GGNNCC 2 cut(s) 404, 717
PstNI CAGNNNCTG 1 cut(s) 363
PsuI RGATCY 1 cut(s) 715
RsaI GTAC 1 cut(s) 614
RsaNI GTAC 1 cut(s) 613
SaqAI TTAA 1 cut(s) 594
SatI GCNGC 4 cut(s) 352, 355, 358, 420
Sau3AI GATC 2 cut(s) 579, 715
SchI GAGTC 2 cut(s) 488, 719
ScrFI CCNGG 3 cut(s) 434, 435, 709
SetI ASST 5 cut(s) 119, 600, 606, 631, 647
SfuI TTCGAA 2 cut(s) 502, 571
SmaI CCCGGG 1 cut(s) 435
Sse9I AATT 5 cut(s) 21, 302, 563, 573, 685
SsiI CCGC 2 cut(s) 220, 420
SspI AATATT 1 cut(s) 63
SspMI CTAG 1 cut(s) 624
StyD4I CCNGG 3 cut(s) 432, 433, 707
TaaI ACNGT 4 cut(s) 169, 365, 402, 494
TaqI TCGA 3 cut(s) 49, 502, 571
TasI AATT 5 cut(s) 21, 302, 563, 573, 685
TauI GCSGC 1 cut(s) 422
TfiI GAWTC 2 cut(s) 55, 504
Tru1I TTAA 1 cut(s) 594
Tru9I TTAA 1 cut(s) 594
TscAI CASTG 1 cut(s) 368
TseFI GTSAC 1 cut(s) 472
TseI GCWGC 3 cut(s) 351, 354, 357
Tsp45I GTSAC 1 cut(s) 472
TspDTI ATGAA 3 cut(s) 44, 556, 695
TspGWI ACGGA 1 cut(s) 491
TspMI CCCGGG 1 cut(s) 433
TspRI CASTG 1 cut(s) 368
XapI RAATTY 5 cut(s) 21, 302, 563, 573, 685
XmaI CCCGGG 1 cut(s) 433
XspI CTAG 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.