Rh5AG235700

Translation initiation factor IF-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0012929
Physical Location & Seq
Reverse (-)
28647685 .. 28649524
1840 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG235700.1

Sequence Viewer

Length: 378 bp
ATGTTAACCTCAACATCATCATCCTCACTCCACCACCCAATCCTCCACTCTCCCCTCCACTCCAAAACCCCAACCCTCACCCCACCCTCCCTCAAATTCCAACCAAACCCAAAACCTCAGCTTCACCTCGGAACCTCAATCCTCATCTCCAAACGTTGTCCCAACATCGTTGCCATGAGTAAATCCCCGAAAGTTGAGGAGCAGAAGTTCAGCGCAGAGGGTCTGGTCACGGAGTCGCTCCCGAACGGCATGTTTCGAATCCGATTAGACAACGCGGACAACATCATAGGATACATTTCTGGGAAAATCCGAAAGAGTTTCATTAGGATTTTGCCTGGGGATAGAGTCAAAGTTGAGGTTTGTTACTGGGAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0009507 GO:0009536 GO:0043021 GO:0043022 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0044877
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

125

Amino Acids

13.94

Weight (kDa)

9.74

Isoelectric Point (pI)

46.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-1a PF01176 73 - 120 1.3e-14 Translation initiation factor 1A / IF-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012929)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G11175
fragaria_vesca FvH4_3g20270
malus_domestica MD11G1244000.v1.1
prunus_persica Prupe.4G182300_v2.0.a1 Prupe.4G182400_v2.0.a1
pyrus_communis pycom03g17460 pycom11g21650
rosa_chinensis RchiOBHm_Chr5g0034161 RchiOBHm_CPg0501881
rosa_laevigata RLG00000033528
rosa_multiflora Rmu_ssc0000128.1_g000028
rosa_roxburghii Rroxscaffold_1G00046120
rosa_rugosa Rorug05G0144300
rosa_samantha Rh5AG235700 Rh5BG236500 Rh5CG266500 Rh5DG243500
rosa_wichuraiana Rw5G021360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 376
AccII CGCG 1 cut(s) 275
AciI CCGC 1 cut(s) 275
AclI AACGTT 1 cut(s) 154
AcsI RAATTY 1 cut(s) 95
AjnI CCWGG 1 cut(s) 334
AloI GAACNNNNNNTCC 2 cut(s) 191, 223
AluBI AGCT 1 cut(s) 121
AluI AGCT 1 cut(s) 121
ApoI RAATTY 1 cut(s) 95
AspLEI GCGC 1 cut(s) 215
AsuHPI GGTGA 2 cut(s) 70, 116
AsuII TTCGAA 1 cut(s) 256
BbvCI CCTCAGC 1 cut(s) 117
BceAI ACGGC 1 cut(s) 262
BciT130I CCWGG 1 cut(s) 336
BciVI GTATCC 1 cut(s) 284
BfuI GTATCC 1 cut(s) 284
Bme1390I CCNGG 1 cut(s) 336
BmiI GGNNCC 1 cut(s) 133
BmrFI CCNGG 1 cut(s) 336
BmrI ACTGGG 1 cut(s) 376
BmuI ACTGGG 1 cut(s) 376
Bpu10I CCTNAGC 1 cut(s) 117
Bpu14I TTCGAA 1 cut(s) 256
BsaJI CCNNGG 2 cut(s) 127, 335
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bse1I ACTGG 1 cut(s) 371
BseBI CCWGG 1 cut(s) 336
BseDI CCNNGG 2 cut(s) 127, 335
BseGI GGATG 1 cut(s) 20
BseMII CTCAG 1 cut(s) 131
BseNI ACTGG 1 cut(s) 371
BseRI GAGGAG 1 cut(s) 212
Bsh1236I CGCG 1 cut(s) 275
BslFI GGGAC 1 cut(s) 144
BsmFI GGGAC 1 cut(s) 144
Bsp119I TTCGAA 1 cut(s) 256
BspACI CCGC 1 cut(s) 275
BspCNI CTCAG 1 cut(s) 130
BspFNI CGCG 1 cut(s) 275
BspLI GGNNCC 1 cut(s) 133
BspT104I TTCGAA 1 cut(s) 256
BsrI ACTGG 1 cut(s) 371
BssECI CCNNGG 2 cut(s) 127, 335
Bst2UI CCWGG 1 cut(s) 336
BstBI TTCGAA 1 cut(s) 256
BstDEI CTNAG 1 cut(s) 117
BstF5I GGATG 1 cut(s) 20
BstFNI CGCG 1 cut(s) 275
BstHHI GCGC 1 cut(s) 215
BstNI CCWGG 1 cut(s) 336
BstNSI RCATGY 1 cut(s) 253
BstSCI CCNGG 1 cut(s) 334
BstUI CGCG 1 cut(s) 275
BsuI GTATCC 1 cut(s) 284
BtsCI GGATG 1 cut(s) 20
CfoI GCGC 1 cut(s) 215
CviAII CATG 2 cut(s) 175, 250
CviJI RGCY 1 cut(s) 121
CviKI_1 RGCY 1 cut(s) 121
DdeI CTNAG 1 cut(s) 117
EcoRII CCWGG 1 cut(s) 334
FaeI CATG 2 cut(s) 178, 253
FaiI YATR 4 cut(s) 176, 251, 287, 376
FaqI GGGAC 1 cut(s) 144
FatI CATG 2 cut(s) 174, 249
FokI GGATG 1 cut(s) 7
GlaI GCGC 1 cut(s) 214
HhaI GCGC 1 cut(s) 215
Hin1II CATG 2 cut(s) 178, 253
Hin6I GCGC 1 cut(s) 213
HinP1I GCGC 1 cut(s) 213
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 3 cut(s) 233, 258, 345
HpaI GTTAAC 1 cut(s) 6
HphI GGTGA 2 cut(s) 70, 116
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 3 cut(s) 131, 263, 311
Hpy188III TCNNGA 1 cut(s) 241
Hpy8I GTNNAC 1 cut(s) 6
HpyCH4IV ACGT 1 cut(s) 154
HpyF3I CTNAG 1 cut(s) 117
HpySE526I ACGT 1 cut(s) 154
Hsp92II CATG 2 cut(s) 178, 253
HspAI GCGC 1 cut(s) 213
KspAI GTTAAC 1 cut(s) 6
LmnI GCTCC 2 cut(s) 199, 243
LpnPI CCDG 5 cut(s) 209, 285, 321, 348, 352
MaeII ACGT 1 cut(s) 154
MaeIII GTNAC 2 cut(s) 226, 362
MluCI AATT 2 cut(s) 95, 371
MlyI GAGTC 2 cut(s) 242, 354
MmeI TCCRAC 1 cut(s) 124
MseI TTAA 1 cut(s) 5
MspR9I CCNGG 1 cut(s) 336
MvaI CCWGG 1 cut(s) 336
MvnI CGCG 1 cut(s) 275
NlaIII CATG 2 cut(s) 178, 253
NlaIV GGNNCC 1 cut(s) 133
NmuCI GTSAC 1 cut(s) 226
NspI RCATGY 1 cut(s) 253
NspV TTCGAA 1 cut(s) 256
PfeI GAWTC 1 cut(s) 258
PleI GAGTC 2 cut(s) 241, 353
PpsI GAGTC 2 cut(s) 241, 353
PsiI TTATAA 1 cut(s) 376
Psp1406I AACGTT 1 cut(s) 154
Psp6I CCWGG 1 cut(s) 334
PspGI CCWGG 1 cut(s) 334
PspN4I GGNNCC 1 cut(s) 133
SaqAI TTAA 1 cut(s) 5
SchI GAGTC 2 cut(s) 242, 354
ScrFI CCNGG 1 cut(s) 336
SetI ASST 7 cut(s) 11, 118, 123, 129, 137, 157, 360
SfuI TTCGAA 1 cut(s) 256
Sse9I AATT 2 cut(s) 95, 371
SsiI CCGC 1 cut(s) 275
StyD4I CCNGG 1 cut(s) 334
TaiI ACGT 1 cut(s) 157
TaqI TCGA 1 cut(s) 256
TasI AATT 2 cut(s) 95, 371
TfiI GAWTC 1 cut(s) 258
Tru1I TTAA 1 cut(s) 5
Tru9I TTAA 1 cut(s) 5
TseFI GTSAC 1 cut(s) 226
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 1 cut(s) 310
TspGWI ACGGA 1 cut(s) 245
XapI RAATTY 1 cut(s) 95
XceI RCATGY 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.