MD11G1244000.v1.1

Translation initiation factor IF-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0012929
Physical Location & Seq
Forward (+)
35504663 .. 35505205
543 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1244000.v1.1.491

Sequence Viewer

Length: 543 bp
ATGTTAACCTCGCCATCATCCTCCCTCCGCTACCCAATCCTCCACCACTGTCCCCCTTTCCACTCTAAAACCCTAACCCTAACCCTCACCCTCACCCCATCAACTCTCAAATTCCAACCAAACCCAAAACCCCAGTCCCATCTCCACATCACCAACAACAGCAGCAGCCACTGTCTTGTCGGAAACCACTTCCTCGTCAGCCCCAAACGGTTCCCGTTCGTTGTCGCCGCGAGCAAATCCCCGGGAGTGGACGAGCAGAAGTTCAGCCAAGAAGGGCTGGTCACGGAGTCGCTCCCCAACGGTATGTTTCGAATCCGATTAGACAACGCCGACACTATCATCGGCTACATTTCTGGGAAAATCCGAAAGAAATTCATTCGAATTTTGATCGGAGACAGAGTTAAGGTCGAGGTCAGCCGGTACGACACCACTAGAGGTCGTATTGTTTTCAGGTATAAACAGGATGACGACCCAAGCAAAAGAAAGATGAAGAATTCCGAAACGAAAAATAAATCCAGGAAGGATCCTATGGACTCTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0009507 GO:0009536 GO:0043021 GO:0043022 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0044877
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

181

Amino Acids

20.45

Weight (kDa)

10.12

Isoelectric Point (pI)

52.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-1a PF01176 90 - 151 4.7e-21 Translation initiation factor 1A / IF-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012929)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G11175
fragaria_vesca FvH4_3g20270
malus_domestica MD11G1244000.v1.1
prunus_persica Prupe.4G182300_v2.0.a1 Prupe.4G182400_v2.0.a1
pyrus_communis pycom03g17460 pycom11g21650
rosa_chinensis RchiOBHm_Chr5g0034161 RchiOBHm_CPg0501881
rosa_laevigata RLG00000033528
rosa_multiflora Rmu_ssc0000128.1_g000028
rosa_roxburghii Rroxscaffold_1G00046120
rosa_rugosa Rorug05G0144300
rosa_samantha Rh5AG235700 Rh5BG236500 Rh5CG266500 Rh5DG243500
rosa_wichuraiana Rw5G021360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 230
AciI CCGC 2 cut(s) 28, 228
AclWI GGATC 2 cut(s) 518, 531
AcsI RAATTY 4 cut(s) 110, 371, 381, 493
AfaI GTAC 1 cut(s) 422
AfiI CCNNNNNNNGG 1 cut(s) 247
AjnI CCWGG 1 cut(s) 515
Alw26I GTCTC 1 cut(s) 387
AlwI GGATC 2 cut(s) 518, 531
AlwNI CAGNNNCTG 1 cut(s) 171
Ama87I CYCGRG 1 cut(s) 241
ApeKI GCWGC 2 cut(s) 162, 165
ApoI RAATTY 4 cut(s) 110, 371, 381, 493
ArsI GACNNNNNNTTYG 2 cut(s) 119, 151
AsuC2I CCSGG 2 cut(s) 242, 243
AsuHPI GGTGA 3 cut(s) 79, 85, 142
AsuII TTCGAA 2 cut(s) 310, 379
AvaI CYCGRG 1 cut(s) 241
BamHI GGATCC 1 cut(s) 523
BbvI GCAGC 2 cut(s) 174, 177
BccI CCATC 3 cut(s) 22, 106, 147
BciT130I CCWGG 1 cut(s) 517
BcnI CCSGG 2 cut(s) 242, 243
BcoDI GTCTC 1 cut(s) 387
BfaI CTAG 1 cut(s) 432
BisI GCNGC 3 cut(s) 163, 166, 228
BlsI GCNGC 3 cut(s) 164, 167, 229
Bme1390I CCNGG 3 cut(s) 242, 243, 517
BmeT110I CYCGRG 1 cut(s) 241
BmiI GGNNCC 2 cut(s) 212, 525
BmrFI CCNGG 3 cut(s) 242, 243, 517
BmrI ACTGGG 1 cut(s) 127
BmuI ACTGGG 1 cut(s) 127
Bpu14I TTCGAA 2 cut(s) 310, 379
BpuMI CCSGG 2 cut(s) 242, 243
BsaJI CCNNGG 2 cut(s) 240, 241
BsaXI ACNNNNNCTCC 2 cut(s) 384, 414
Bsc4I CCNNNNNNNGG 1 cut(s) 247
Bse118I RCCGGY 1 cut(s) 417
Bse1I ACTGG 1 cut(s) 133
BseBI CCWGG 1 cut(s) 517
BseDI CCNNGG 2 cut(s) 240, 241
BseGI GGATG 2 cut(s) 17, 469
BseLI CCNNNNNNNGG 1 cut(s) 247
BseNI ACTGG 1 cut(s) 133
BseXI GCAGC 2 cut(s) 174, 177
Bsh1236I CGCG 1 cut(s) 230
BsiHKCI CYCGRG 1 cut(s) 241
BsiSI CCGG 2 cut(s) 242, 418
BslFI GGGAC 2 cut(s) 36, 121
BslI CCNNNNNNNGG 1 cut(s) 247
BsmAI GTCTC 1 cut(s) 387
BsmFI GGGAC 2 cut(s) 36, 121
BsoBI CYCGRG 1 cut(s) 241
Bsp119I TTCGAA 2 cut(s) 310, 379
Bsp143I GATC 2 cut(s) 387, 523
BspACI CCGC 2 cut(s) 28, 228
BspFNI CGCG 1 cut(s) 230
BspLI GGNNCC 2 cut(s) 212, 525
BspPI GGATC 2 cut(s) 518, 531
BspT104I TTCGAA 2 cut(s) 310, 379
BsrFI RCCGGY 1 cut(s) 417
BsrI ACTGG 1 cut(s) 133
BssAI RCCGGY 1 cut(s) 417
BssECI CCNNGG 2 cut(s) 240, 241
BssMI GATC 2 cut(s) 387, 523
Bst2UI CCWGG 1 cut(s) 517
Bst4CI ACNGT 4 cut(s) 50, 173, 210, 302
BstBI TTCGAA 2 cut(s) 310, 379
BstC8I GCNNGC 1 cut(s) 232
BstF5I GGATG 2 cut(s) 17, 469
BstFNI CGCG 1 cut(s) 230
BstKTI GATC 2 cut(s) 390, 526
BstMAI GTCTC 1 cut(s) 387
BstMBI GATC 2 cut(s) 387, 523
BstNI CCWGG 1 cut(s) 517
BstSCI CCNGG 3 cut(s) 240, 241, 515
BstUI CGCG 1 cut(s) 230
BstV1I GCAGC 2 cut(s) 174, 177
BstX2I RGATCY 1 cut(s) 523
BstYI RGATCY 1 cut(s) 523
BtsCI GGATG 2 cut(s) 17, 469
BtsIMutI CAGTG 2 cut(s) 46, 169
Cac8I GCNNGC 1 cut(s) 232
CaiI CAGNNNCTG 1 cut(s) 171
Cfr10I RCCGGY 1 cut(s) 417
Cfr9I CCCGGG 1 cut(s) 241
Csp6I GTAC 1 cut(s) 421
CviJI RGCY 6 cut(s) 168, 201, 267, 277, 345, 417
CviKI_1 RGCY 6 cut(s) 168, 201, 267, 277, 345, 417
CviQI GTAC 1 cut(s) 421
DpnI GATC 2 cut(s) 389, 525
DpnII GATC 2 cut(s) 387, 523
Eco88I CYCGRG 1 cut(s) 241
EcoRI GAATTC 1 cut(s) 493
EcoRII CCWGG 1 cut(s) 515
FaiI YATR 3 cut(s) 305, 456, 530
FaqI GGGAC 2 cut(s) 36, 121
Fnu4HI GCNGC 3 cut(s) 163, 166, 228
FokI GGATG 2 cut(s) 4, 476
Fsp4HI GCNGC 3 cut(s) 163, 166, 228
FspBI CTAG 1 cut(s) 432
GluI GCNGC 3 cut(s) 163, 166, 228
HapII CCGG 2 cut(s) 242, 418
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 3 cut(s) 287, 312, 533
HpaI GTTAAC 1 cut(s) 6
HpaII CCGG 2 cut(s) 242, 418
HphI GGTGA 3 cut(s) 79, 85, 142
Hpy166II GTNNAC 2 cut(s) 6, 250
Hpy188I TCNGA 5 cut(s) 182, 317, 365, 392, 499
Hpy8I GTNNAC 2 cut(s) 6, 250
HpyAV CCTTC 2 cut(s) 266, 514
HpyCH4III ACNGT 4 cut(s) 50, 173, 210, 302
KspAI GTTAAC 1 cut(s) 6
Kzo9I GATC 2 cut(s) 387, 523
LmnI GCTCC 1 cut(s) 297
LpnPI CCDG 9 cut(s) 146, 255, 263, 339, 431, 436, 446, 502, 529
Lsp1109I GCAGC 2 cut(s) 174, 177
MaeI CTAG 1 cut(s) 432
MaeIII GTNAC 1 cut(s) 280
MalI GATC 2 cut(s) 389, 525
MboI GATC 2 cut(s) 387, 523
MboII GAAGA 1 cut(s) 502
MflI RGATCY 1 cut(s) 523
MluCI AATT 4 cut(s) 110, 371, 381, 493
MlyI GAGTC 2 cut(s) 296, 527
MmeI TCCRAC 2 cut(s) 139, 160
MnlI CCTC 9 cut(s) 19, 31, 35, 50, 95, 101, 203, 403, 428
MseI TTAA 2 cut(s) 5, 402
MspI CCGG 2 cut(s) 242, 418
MspR9I CCNGG 3 cut(s) 242, 243, 517
MvaI CCWGG 1 cut(s) 517
MvnI CGCG 1 cut(s) 230
NciI CCSGG 2 cut(s) 242, 243
NdeII GATC 2 cut(s) 387, 523
NlaIV GGNNCC 2 cut(s) 212, 525
NmuCI GTSAC 1 cut(s) 280
NspV TTCGAA 2 cut(s) 310, 379
PfeI GAWTC 1 cut(s) 312
PfoI TCCNGGA 1 cut(s) 515
PkrI GCNGC 3 cut(s) 164, 167, 229
PleI GAGTC 2 cut(s) 295, 527
PpsI GAGTC 2 cut(s) 295, 527
Psp6I CCWGG 1 cut(s) 515
PspGI CCWGG 1 cut(s) 515
PspN4I GGNNCC 2 cut(s) 212, 525
PstNI CAGNNNCTG 1 cut(s) 171
PsuI RGATCY 1 cut(s) 523
RsaI GTAC 1 cut(s) 422
RsaNI GTAC 1 cut(s) 421
SaqAI TTAA 2 cut(s) 5, 402
SatI GCNGC 3 cut(s) 163, 166, 228
Sau3AI GATC 2 cut(s) 387, 523
SchI GAGTC 2 cut(s) 296, 527
ScrFI CCNGG 3 cut(s) 242, 243, 517
SetI ASST 5 cut(s) 11, 408, 414, 439, 455
SfuI TTCGAA 2 cut(s) 310, 379
SmaI CCCGGG 1 cut(s) 243
Sse9I AATT 4 cut(s) 110, 371, 381, 493
SsiI CCGC 2 cut(s) 28, 228
SspMI CTAG 1 cut(s) 432
StyD4I CCNGG 3 cut(s) 240, 241, 515
TaaI ACNGT 4 cut(s) 50, 173, 210, 302
TaqI TCGA 3 cut(s) 310, 379, 408
TasI AATT 4 cut(s) 110, 371, 381, 493
TauI GCSGC 1 cut(s) 230
TfiI GAWTC 1 cut(s) 312
Tru1I TTAA 2 cut(s) 5, 402
Tru9I TTAA 2 cut(s) 5, 402
TscAI CASTG 2 cut(s) 53, 176
TseFI GTSAC 1 cut(s) 280
TseI GCWGC 2 cut(s) 162, 165
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 2 cut(s) 364, 503
TspGWI ACGGA 1 cut(s) 299
TspMI CCCGGG 1 cut(s) 241
TspRI CASTG 2 cut(s) 53, 176
XapI RAATTY 4 cut(s) 110, 371, 381, 493
XmaI CCCGGG 1 cut(s) 241
XspI CTAG 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.