Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0003672

Physical Location & Seq

Genomic Coordinates Forward (+)

16289944 .. 16300651

Gene Structure 10708 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g23220.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2682 bp

ATGACTTCACTTTCACTCTCAACTAACCTCACTCTGTACCCCAATCTCACATTTCTCCAATCCCACTATCCAAACAGACCCCAAAGAATCACCTTCCCTTTGAAGAAGTTGAAAACCCAGATAGGCTGCTCCGCCACAGGTCAACAAAGACCCCCCAAAAAGAAGAAAACCCCTTCCGACGAGAAAGGCGTCGACCCAGTTGGCTTCCTCTCCAAATTCGACATTTCCCATAAGCAATTCGCTCAGTTCCTCCGTGAAAGACACAAAGCCATGAAGGAGCTGACAGATGAAATATTGAATCGGCACATCAACCTCAGGGATATGTCTTCTGGATATGAGATATTGGGTTTGCACCGACACCCAGAACATAGAGTGGATTATATGGAGTGGGCTCCAGGTGCTCGCTACTGCGCACTGGTTGGTGATTTCAATGGATGGTCTCCCACAGAGAACTGTGCAAAAGAGGGTCATTTTGGACATGATGACTATGGATACTGGTTCATCATTCTTGAAGATAAGTTGAGGAATGGAGAGCAACCAGATGAGCTGTACTTTCAGCAGTATAACTATGTAGATGATTATGATAAAGGTGACAGTGGTGTGACCATTGAAGAAGTCTTTAAGAAAGCAAATGATGAGTATTGGGAACCCGGAGAAGACCGGTTTCTAAAAAACCGTTTAGAAGTACCAGCAAAGTTGTATGAGCAGATTTTTGGGCCTAATGGACCTCAAACGTTAGAGGAACTTGAAGAAATACCAGATGCAGAAACAAGATACAAAGCGTGGAAAGAACAGCATAAAGATGACCCGCCTAGTAACTTACCTTCTTATGATGTGATTGACAGTGGAAAAGAGTATGACATTTACAATGTTGTGCTTGATCCAGTATCAGTAGAGAAATTTAAAACAAAGAAGCCTCCCTTGCCATATTGGTTCGAGACACGTAAGGGAAGGAAGGCATGGTTGAAAAAGTATAGGCCGTGCATTCCTCATGGGAGCAAGTACAGGGTCTATTTTAACACTCCTAGTGGGCCACTGGAACGAGTACCTGCTTGGGCAACTTATGTGGAACCAGACGCAGATGGAGGACAAGCTTTTGCAATCCACTGGGAACCACCCCCAGAAATTGCATACAAATGGAAGAACGCACGCCCTAAAGTGCCAAAGTCCCTGCGCATTTATGAATGCCATGTTGGCATAAGTGGATCAGAGCCTAAAGTATCTTCTTTCAGTGATTTCACAGAGAAGGTTCTTCCTCATGTCAAGGAGGCTGGCTATAATGCAATTCAGCTGATTGGAGTTATTGAGCACAAAGATTATTTCACTGTTGGTTATAGAGCCACTAATTTATATGCTGTTAGCAGCCGATTTGGCACCCCTGATGATTTCAAGCGTTTGGTTGATGAAGCACATGGACAAGGACTGCTGGTCTTCTTGGAGATTGTCCATTCCTACTCAGCTGCAGATGAGATGGTTGGATTAGCGCTTTTCGATGGAACAAATGACTGCTACTTCCACACTGGCAAAAGGGGACATCATAAGCACTGGGGTACCAGAATGTTTAAATATGGTGATCTTGATGTTTTGCATTTCCTTCTCTCGAATTTGAACTGGTGGATTACAGAGTACCAAGTTGATGGTTTTCAGTTCCATTCACTCTCATCAATGATGTATACACATAATGGCTTTGCTTCGTTTACTGGTGACTTGGAGGAGTACTGCAATCAATATGTTGATAGGGATGCATTGTTATACCTCATGTTAGCAAACGAGATACTTCATGCTCTCCACCCAGATATAATAACAATTGGTGAAGATGCAACCTTCTATCCAGGTCTATGTGAACCAATCTCTCAAGGCGGATTGGGCTTTGATTATTGCGTCAATCTTTCTGTGTCAGAGATGTGGTCATCTTTCCTTGAGAATGTTCCGGACCATGATTGGAGCATGAGTAAGATTGTGAGCGCATTAATGGGCAACAAAAACTATGCGGATAAGATGCTCATGTATGCTGAAAATCATAACCAGTCAATCTCTGGAGGGAGATCCTTTGCTGAGATACTGTTTGGTGAAATCAGGGAGGCCTCTCCTGACAAGGAGAAATTATTGCTCCGAGGTTGTTCCTTGCACAAGATGATTAGATTAATTACCATGACAATTGGTGGCCGTGCTTATCTCAACTTCATGGGCAATGAATTTGGGCATCCAGAGAGGGTTGAGTTCCCAATGCCAAGCAACAATTTCTCGTTTTCACTTGCCAAGCGAAGGTGGGATCTTCTTGCCAATGAGGGATTGCATCGCGACTTGTTTGCCTTCGATAAGGACTTGATGAAGTTGGATGAAAATGAAAGAATACTTGCAAGAGTTTTACCCAGCATTCACCATGTGAATGACAATAGCATGGTAATTGCTTATATCAGAGGGCCTCTTCTCCTCGTATTTAACTTCCATCCAACAGATTCTTATGAAGGATACCAAATAGGTGTAGAAGAAGCTGGAGAGTATCAACTTGTACTAAATACGGATGAAAGAAAGTACGGAGGTCAGGGGCTTATGAAGGATGACCAATATTCTAGGACAACTACTAGTCAAAGAACTGATGGCCTCCGAAACAGCTTAGAAGTTCCTCTGCCTAGTAGGACTGCCCAGGTTTACAAATTGAGTCGGATTTTAAGAATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0007275 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009791 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0048856 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

894

Amino Acids

102.81

Weight (kDa)

5.92

Isoelectric Point (pI)

35.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBM_48	PF02922	115 - 177	1.4e-06	Carbohydrate-binding module 48 (Isoamylase N-terminal domain)
Alpha-amylase	PF00128	418 - 486	9.8e-07	Alpha amylase, catalytic domain
Alpha-amylase_C	PF02806	795 - 887	1.4e-21	Alpha amylase, C-terminal all-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003672)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20440 AT3G20440 AT3G20440
fragaria_vesca	FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220
malus_domestica	MD11G1212700.v1.1
prunus_persica	Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1
pyrus_communis	pycom11g18770
rosa_chinensis	RchiOBHm_Chr5g0040901 RchiOBHm_Chr5g0040911
rosa_laevigata	RLG00000034012
rosa_multiflora	Rmu_sc0002848.1_g000016 Rmu_ssc0000416.1_g000003
rosa_roxburghii	Rroxscaffold_1G00039790
rosa_rugosa	Rorug05G0188500 Rorug05G0188600 Rorug05G0188600
rosa_samantha	Rh5AG274100 Rh5BG279100 Rh5CG311200 Rh5DG287300
rosa_wichuraiana	Rw5G025750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	412, 1175
Acc36I	ACCTGC	1 cut(s)	1057
Acc65I	GGTACC	1 cut(s)	1550
AccB1I	GGYRCC	2 cut(s)	1373, 1550
AccI	GTMKAC	2 cut(s)	192, 1673
AccII	CGCG	1 cut(s)	2301
AccIII	TCCGGA	1 cut(s)	1930
AciI	CCGC	4 cut(s)	132, 809, 1860, 1991
AclI	AACGTT	1 cut(s)	734
AclWI	GGATC	4 cut(s)	875, 1213, 2040, 2280
AcoI	YGGCCR	1 cut(s)	2164
AcsI	RAATTY	4 cut(s)	215, 899, 1603, 2195
AcyI	GRCGYC	1 cut(s)	189
AdeI	CACNNNGTG	1 cut(s)	2386
AfeI	AGCGCT	1 cut(s)	1485
AfiI	CCNNNNNNNGG	1 cut(s)	2265
AflIII	ACRYGT	1 cut(s)	941
AgeI	ACCGGT	1 cut(s)	660
AhdI	GACNNNNNGTC	1 cut(s)	1427
AhlI	ACTAGT	1 cut(s)	2585
AjnI	CCWGG	3 cut(s)	394, 1831, 2646
AjuI	GAANNNNNNNTTGG	2 cut(s)	1176, 1208
AluBI	AGCT	7 cut(s)	280, 547, 1094, 1291, 1460, 2495, 2616
AluI	AGCT	7 cut(s)	280, 547, 1094, 1291, 1460, 2495, 2616
Alw21I	GWGCWC	2 cut(s)	403, 1311
Alw26I	GTCTC	2 cut(s)	444, 932
AlwI	GGATC	4 cut(s)	875, 1213, 2040, 2280
Aor13HI	TCCGGA	1 cut(s)	1930
Aor51HI	AGCGCT	1 cut(s)	1485
AoxI	GGCC	7 cut(s)	716, 977, 1031, 2082, 2164, 2423, 2602
ApeKI	GCWGC	3 cut(s)	126, 1362, 1460
ApoI	RAATTY	4 cut(s)	215, 899, 1603, 2195
AseI	ATTAAT	2 cut(s)	1970, 2144
AsiGI	ACCGGT	1 cut(s)	660
Asp718I	GGTACC	1 cut(s)	1550
AspLEI	GCGC	4 cut(s)	413, 1176, 1486, 1967
AspS9I	GGNCC	5 cut(s)	716, 725, 1031, 1933, 2423
AsuC2I	CCSGG	1 cut(s)	651
AsuHPI	GGTGA	8 cut(s)	82, 434, 602, 1583, 1715, 1823, 2081, 2372
AvaII	GGWCC	2 cut(s)	725, 1933
AxyI	CCTNAGG	1 cut(s)	314
BaeI	ACNNNNGTAYC	2 cut(s)	1534, 1567
BanI	GGYRCC	2 cut(s)	1373, 1550
BanII	GRGCYC	1 cut(s)	394
BarI	GAAGNNNNNNTAC	2 cut(s)	808, 840
BbsI	GAAGAC	3 cut(s)	318, 663, 1423
Bbv12I	GWGCWC	2 cut(s)	403, 1311
BbvI	GCAGC	3 cut(s)	113, 1374, 1447
BccI	CCATC	7 cut(s)	429, 1076, 1465, 1487, 1631, 2457, 2594
BceAI	ACGGC	2 cut(s)	964, 2151
BcgI	CGANNNNNNTGC	2 cut(s)	2291, 2325
BciT130I	CCWGG	3 cut(s)	396, 1833, 2648
BciVI	GTATCC	2 cut(s)	485, 2465
BcnI	CCSGG	1 cut(s)	651
BcoDI	GTCTC	2 cut(s)	444, 932
BcuI	ACTAGT	1 cut(s)	2585
BfaI	CTAG	5 cut(s)	813, 1026, 2574, 2586, 2634
BfmI	CTRYAG	1 cut(s)	1461
BfoI	RGCGCY	1 cut(s)	1487
BfuAI	ACCTGC	1 cut(s)	1057
BfuI	GTATCC	2 cut(s)	485, 2465
BglI	GCCNNNNNGGC	2 cut(s)	1194, 1371
BisI	GCNGC	3 cut(s)	127, 1363, 1461
BlsI	GCNGC	3 cut(s)	128, 1364, 1462
BmcAI	AGTACT	1 cut(s)	1718
Bme1390I	CCNGG	4 cut(s)	396, 651, 1833, 2648
Bme18I	GGWCC	2 cut(s)	725, 1933
BmeRI	GACNNNNNGTC	1 cut(s)	1427
BmgT120I	GGNCC	5 cut(s)	716, 725, 1031, 1933, 2423
BmiI	GGNNCC	6 cut(s)	393, 648, 1071, 1113, 1375, 1552
BmrFI	CCNGG	4 cut(s)	396, 651, 1833, 2648
BmrI	ACTGGG	3 cut(s)	191, 1117, 1555
BmsI	GCATC	6 cut(s)	751, 1732, 1807, 1989, 2212, 2305
BmuI	ACTGGG	3 cut(s)	191, 1117, 1555
BpiI	GAAGAC	3 cut(s)	318, 663, 1423
BpmI	CTGGAG	3 cut(s)	378, 2058, 2517
BpuEI	CTTGAG	2 cut(s)	1839, 1940
BpuMI	CCSGG	1 cut(s)	651
BsaAI	YACGTR	1 cut(s)	944
BsaHI	GRCGYC	1 cut(s)	189
BsaI	GGTCTC	1 cut(s)	444
BsaJI	CCNNGG	2 cut(s)	2113, 2646
BsaWI	WCCGGW	2 cut(s)	660, 1930
BsaXI	ACNNNNNCTCC	2 cut(s)	1936, 1966
Bsc4I	CCNNNNNNNGG	1 cut(s)	2265
Bse118I	RCCGGY	1 cut(s)	660
Bse21I	CCTNAGG	1 cut(s)	314
Bse3DI	GCAATG	1 cut(s)	2197
BseAI	TCCGGA	1 cut(s)	1930
BseBI	CCWGG	3 cut(s)	396, 1833, 2648
BseDI	CCNNGG	2 cut(s)	2113, 2646
BseGI	GGATG	7 cut(s)	440, 1747, 2203, 2344, 2449, 2530, 2566
BseLI	CCNNNNNNNGG	1 cut(s)	2265
BseMI	GCAATG	1 cut(s)	2197
BseMII	CTCAG	4 cut(s)	257, 328, 1470, 2046
BseRI	GAGGAG	2 cut(s)	1727, 2423
BseXI	GCAGC	3 cut(s)	113, 1374, 1447
BseYI	CCCAGC	1 cut(s)	2372
Bsh1236I	CGCG	1 cut(s)	2301
BshFI	GGCC	7 cut(s)	718, 979, 1033, 2084, 2166, 2425, 2604
BshNI	GGYRCC	2 cut(s)	1373, 1550
BshTI	ACCGGT	1 cut(s)	660
BsiHKAI	GWGCWC	2 cut(s)	403, 1311
BsiSI	CCGG	3 cut(s)	651, 661, 1931
BslFI	GGGAC	2 cut(s)	1153, 1545
BslI	CCNNNNNNNGG	1 cut(s)	2265
BsmAI	GTCTC	2 cut(s)	444, 932
BsmFI	GGGAC	2 cut(s)	1153, 1545
BsmI	GAATGC	3 cut(s)	984, 1190, 2376
BsnI	GGCC	7 cut(s)	718, 979, 1033, 2084, 2166, 2425, 2604
Bso31I	GGTCTC	1 cut(s)	444
Bsp1286I	GDGCHC	3 cut(s)	394, 403, 1311
Bsp13I	TCCGGA	1 cut(s)	1930
Bsp143I	GATC	5 cut(s)	880, 1205, 1573, 2045, 2272
Bsp68I	TCGCGA	1 cut(s)	2301
BspACI	CCGC	4 cut(s)	132, 809, 1860, 1991
BspANI	GGCC	7 cut(s)	718, 979, 1033, 2084, 2166, 2425, 2604
BspCNI	CTCAG	4 cut(s)	256, 327, 1469, 2047
BspEI	TCCGGA	1 cut(s)	1930
BspFNI	CGCG	1 cut(s)	2301
BspLI	GGNNCC	6 cut(s)	393, 648, 1071, 1113, 1375, 1552
BspMAI	CTGCAG	1 cut(s)	1465
BspMI	ACCTGC	1 cut(s)	1057
BspPI	GGATC	4 cut(s)	875, 1213, 2040, 2280
BspT107I	GGYRCC	2 cut(s)	1373, 1550
BspTNI	GGTCTC	1 cut(s)	444
BsrDI	GCAATG	1 cut(s)	2197
BsrFI	RCCGGY	1 cut(s)	660
BssAI	RCCGGY	1 cut(s)	660
BssECI	CCNNGG	2 cut(s)	2113, 2646
BssMI	GATC	5 cut(s)	880, 1205, 1573, 2045, 2272
BssNAI	GTATAC	1 cut(s)	1674
BssNI	GRCGYC	1 cut(s)	189
Bst1107I	GTATAC	1 cut(s)	1674
Bst2UI	CCWGG	3 cut(s)	396, 1833, 2648
Bst4CI	ACNGT	6 cut(s)	455, 596, 677, 845, 1327, 2064
Bst6I	CTCTTC	1 cut(s)	2433
BstACI	GRCGYC	1 cut(s)	189
BstBAI	YACGTR	1 cut(s)	944
BstC8I	GCNNGC	3 cut(s)	403, 1150, 1273
BstDEI	CTNAG	5 cut(s)	243, 314, 1456, 2055, 2617
BstF5I	GGATG	7 cut(s)	440, 1747, 2203, 2344, 2449, 2530, 2566
BstFNI	CGCG	1 cut(s)	2301
BstH2I	RGCGCY	1 cut(s)	1487
BstHHI	GCGC	4 cut(s)	413, 1176, 1486, 1967
BstKTI	GATC	5 cut(s)	883, 1208, 1576, 2048, 2275
BstMAI	GTCTC	2 cut(s)	444, 932
BstMBI	GATC	5 cut(s)	880, 1205, 1573, 2045, 2272
BstMWI	GCNNNNNNNGC	5 cut(s)	398, 922, 1194, 1371, 1866
BstNI	CCWGG	3 cut(s)	396, 1833, 2648
BstSCI	CCNGG	4 cut(s)	394, 649, 1831, 2646
BstSFI	CTRYAG	1 cut(s)	1461
BstUI	CGCG	1 cut(s)	2301
BstV1I	GCAGC	3 cut(s)	113, 1374, 1447
BstV2I	GAAGAC	3 cut(s)	318, 663, 1423
BstX2I	RGATCY	2 cut(s)	2045, 2272
BstXI	CCANNNNNNTGG	1 cut(s)	1637
BstYI	RGATCY	2 cut(s)	2045, 2272
BstZ17I	GTATAC	1 cut(s)	1674
Bsu36I	CCTNAGG	1 cut(s)	314
BsuI	GTATCC	2 cut(s)	485, 2465
BsuRI	GGCC	7 cut(s)	718, 979, 1033, 2084, 2166, 2425, 2604
BtgZI	GCGATG	1 cut(s)	2282
BtsCI	GGATG	7 cut(s)	440, 1747, 2203, 2344, 2449, 2530, 2566
BtsIMutI	CAGTG	9 cut(s)	413, 601, 850, 1034, 1105, 1237, 1323, 1518, 1543
BtuMI	TCGCGA	1 cut(s)	2301
BveI	ACCTGC	1 cut(s)	1057
Cac8I	GCNNGC	3 cut(s)	403, 1150, 1273
CfoI	GCGC	4 cut(s)	413, 1176, 1486, 1967
Cfr10I	RCCGGY	1 cut(s)	660
Cfr13I	GGNCC	5 cut(s)	716, 725, 1031, 1933, 2423
CseI	GACGC	3 cut(s)	178, 1085, 1870
CspAI	ACCGGT	1 cut(s)	660
CspCI	CAANNNNNGTGG	2 cut(s)	1047, 1082
DdeI	CTNAG	5 cut(s)	243, 314, 1456, 2055, 2617
DpnI	GATC	5 cut(s)	882, 1207, 1575, 2047, 2274
DpnII	GATC	5 cut(s)	880, 1205, 1573, 2045, 2272
DraI	TTTAAA	2 cut(s)	904, 1564
DraIII	CACNNNGTG	1 cut(s)	2386
DriI	GACNNNNNGTC	1 cut(s)	1427
EaeI	YGGCCR	1 cut(s)	2164
Eam1104I	CTCTTC	1 cut(s)	2433
Eam1105I	GACNNNNNGTC	1 cut(s)	1427
EarI	CTCTTC	1 cut(s)	2433
EciI	GGCGGA	2 cut(s)	121, 1875
Eco147I	AGGCCT	1 cut(s)	2084
Eco24I	GRGCYC	1 cut(s)	394
Eco31I	GGTCTC	1 cut(s)	444
Eco47I	GGWCC	2 cut(s)	725, 1933
Eco47III	AGCGCT	1 cut(s)	1485
Eco81I	CCTNAGG	1 cut(s)	314
EcoO109I	RGGNCCY	1 cut(s)	2423
EcoRII	CCWGG	3 cut(s)	394, 1831, 2646
EcoT22I	ATGCAT	1 cut(s)	1747
EcoT38I	GRGCYC	1 cut(s)	394
FalI	AAGNNNNNCTT	2 cut(s)	905, 937
FaqI	GGGAC	2 cut(s)	1153, 1545
FauI	CCCGC	1 cut(s)	816
FblI	GTMKAC	2 cut(s)	192, 1673
Fnu4HI	GCNGC	3 cut(s)	127, 1363, 1461
FokI	GGATG	7 cut(s)	447, 1754, 2190, 2351, 2436, 2537, 2573
FriOI	GRGCYC	1 cut(s)	394
Fsp4HI	GCNGC	3 cut(s)	127, 1363, 1461
FspBI	CTAG	5 cut(s)	813, 1026, 2574, 2586, 2634
FspI	TGCGCA	2 cut(s)	412, 1175
GlaI	GCGC	4 cut(s)	412, 1175, 1485, 1966
GluI	GCNGC	3 cut(s)	127, 1363, 1461
GsaI	CCCAGC	1 cut(s)	2376
GsuI	CTGGAG	3 cut(s)	378, 2058, 2517
HaeII	RGCGCY	1 cut(s)	1487
HaeIII	GGCC	7 cut(s)	718, 979, 1033, 2084, 2166, 2425, 2604
HapII	CCGG	3 cut(s)	651, 661, 1931
HgaI	GACGC	3 cut(s)	178, 1085, 1870
HhaI	GCGC	4 cut(s)	413, 1176, 1486, 1967
Hin1I	GRCGYC	1 cut(s)	189
Hin6I	GCGC	4 cut(s)	411, 1174, 1484, 1965
HinP1I	GCGC	4 cut(s)	411, 1174, 1484, 1965
HincII	GTYRAC	2 cut(s)	143, 193
HindII	GTYRAC	2 cut(s)	143, 193
HindIII	AAGCTT	1 cut(s)	1092
HinfI	GANTC	4 cut(s)	87, 298, 2459, 2662
HpaII	CCGG	3 cut(s)	651, 661, 1931
HphI	GGTGA	8 cut(s)	82, 434, 602, 1583, 1715, 1823, 2081, 2372
Hpy166II	GTNNAC	6 cut(s)	143, 193, 1674, 1698, 1844, 2653
Hpy188I	TCNGA	7 cut(s)	178, 1210, 1900, 2114, 2420, 2609, 2667
Hpy8I	GTNNAC	6 cut(s)	143, 193, 1674, 1698, 1844, 2653
Hpy99I	CGWCG	2 cut(s)	182, 194
HpyCH4III	ACNGT	6 cut(s)	455, 596, 677, 845, 1327, 2064
HpyCH4IV	ACGT	2 cut(s)	734, 943
HpyF10VI	GCNNNNNNNGC	5 cut(s)	398, 922, 1194, 1371, 1866
HpyF3I	CTNAG	5 cut(s)	243, 314, 1456, 2055, 2617
HpySE526I	ACGT	2 cut(s)	734, 943
Hsp92I	GRCGYC	1 cut(s)	189
HspAI	GCGC	4 cut(s)	411, 1174, 1484, 1965
Kpn2I	TCCGGA	1 cut(s)	1930
KpnI	GGTACC	1 cut(s)	1554
Kzo9I	GATC	5 cut(s)	880, 1205, 1573, 2045, 2272
LmnI	GCTCC	6 cut(s)	134, 277, 397, 996, 1944, 2115
Lsp1109I	GCAGC	3 cut(s)	113, 1374, 1447
LweI	GCATC	6 cut(s)	751, 1732, 1807, 1989, 2212, 2305
MaeI	CTAG	5 cut(s)	813, 1026, 2574, 2586, 2634
MaeII	ACGT	2 cut(s)	734, 943
MaeIII	GTNAC	4 cut(s)	590, 601, 815, 1703
MalI	GATC	5 cut(s)	882, 1207, 1575, 2047, 2274
MboI	GATC	5 cut(s)	880, 1205, 1573, 2045, 2272
MfeI	CAATTG	2 cut(s)	1806, 2157
MflI	RGATCY	2 cut(s)	2045, 2272
MhlI	GDGCHC	3 cut(s)	394, 403, 1311
MlyI	GAGTC	1 cut(s)	2671
MmeI	TCCRAC	5 cut(s)	201, 1456, 2316, 2477, 2645
Mph1103I	ATGCAT	1 cut(s)	1747
MroI	TCCGGA	1 cut(s)	1930
MseI	TTAA	8 cut(s)	621, 903, 1017, 1563, 1970, 2144, 2442, 2672
MslI	CAYNNNNRTG	3 cut(s)	801, 1135, 2388
MspA1I	CMGCKG	2 cut(s)	1291, 1460
MspI	CCGG	3 cut(s)	651, 661, 1931
MspR9I	CCNGG	4 cut(s)	396, 651, 1833, 2648
MunI	CAATTG	2 cut(s)	1806, 2157
Mva1269I	GAATGC	3 cut(s)	984, 1190, 2376
MvaI	CCWGG	3 cut(s)	396, 1833, 2648
MvnI	CGCG	1 cut(s)	2301
MwoI	GCNNNNNNNGC	5 cut(s)	398, 922, 1194, 1371, 1866
NciI	CCSGG	1 cut(s)	651
NdeII	GATC	5 cut(s)	880, 1205, 1573, 2045, 2272
NlaIV	GGNNCC	6 cut(s)	393, 648, 1071, 1113, 1375, 1552
NmuCI	GTSAC	3 cut(s)	590, 601, 1703
NruI	TCGCGA	1 cut(s)	2301
NsbI	TGCGCA	2 cut(s)	412, 1175
NsiI	ATGCAT	1 cut(s)	1747
PceI	AGGCCT	1 cut(s)	2084
PcsI	WCGNNNNNNNCGW	1 cut(s)	186
PctI	GAATGC	3 cut(s)	984, 1190, 2376
PfeI	GAWTC	3 cut(s)	87, 298, 2459
PinAI	ACCGGT	1 cut(s)	660
PkrI	GCNGC	3 cut(s)	128, 1364, 1462
PleI	GAGTC	1 cut(s)	2670
PpsI	GAGTC	1 cut(s)	2670
Ppu21I	YACGTR	1 cut(s)	944
PshBI	ATTAAT	2 cut(s)	1970, 2144
Psp1406I	AACGTT	1 cut(s)	734
Psp6I	CCWGG	3 cut(s)	394, 1831, 2646
PspFI	CCCAGC	1 cut(s)	2372
PspGI	CCWGG	3 cut(s)	394, 1831, 2646
PspN4I	GGNNCC	6 cut(s)	393, 648, 1071, 1113, 1375, 1552
PspPI	GGNCC	5 cut(s)	716, 725, 1031, 1933, 2423
PstI	CTGCAG	1 cut(s)	1465
PsuI	RGATCY	2 cut(s)	2045, 2272
PvuII	CAGCTG	2 cut(s)	1291, 1460
RruI	TCGCGA	1 cut(s)	2301
RseI	CAYNNNNRTG	3 cut(s)	801, 1135, 2388
SalI	GTCGAC	1 cut(s)	191
SaqAI	TTAA	8 cut(s)	621, 903, 1017, 1563, 1970, 2144, 2442, 2672
SatI	GCNGC	3 cut(s)	127, 1363, 1461
Sau3AI	GATC	5 cut(s)	880, 1205, 1573, 2045, 2272
Sau96I	GGNCC	5 cut(s)	716, 725, 1031, 1933, 2423
ScaI	AGTACT	1 cut(s)	1718
SchI	GAGTC	1 cut(s)	2671
ScrFI	CCNGG	4 cut(s)	396, 651, 1833, 2648
SduI	GDGCHC	3 cut(s)	394, 403, 1311
SfaNI	GCATC	6 cut(s)	751, 1732, 1807, 1989, 2212, 2305
SfcI	CTRYAG	1 cut(s)	1461
SinI	GGWCC	2 cut(s)	725, 1933
SmiMI	CAYNNNNRTG	3 cut(s)	801, 1135, 2388
SmlI	CTYRAG	2 cut(s)	1854, 1919
SmoI	CTYRAG	2 cut(s)	1854, 1919
SpeI	ACTAGT	1 cut(s)	2585
SseBI	AGGCCT	1 cut(s)	2084
SsiI	CCGC	4 cut(s)	132, 809, 1860, 1991
SspI	AATATT	2 cut(s)	294, 2570
SspMI	CTAG	5 cut(s)	813, 1026, 2574, 2586, 2634
StuI	AGGCCT	1 cut(s)	2084
StyD4I	CCNGG	4 cut(s)	394, 649, 1831, 2646
TaaI	ACNGT	6 cut(s)	455, 596, 677, 845, 1327, 2064
TaiI	ACGT	2 cut(s)	737, 946
TaqI	TCGA	6 cut(s)	192, 219, 936, 1491, 1601, 2316
TatI	WGTACW	4 cut(s)	549, 1002, 1716, 2512
TfiI	GAWTC	3 cut(s)	87, 298, 2459
Tru1I	TTAA	8 cut(s)	621, 903, 1017, 1563, 1970, 2144, 2442, 2672
Tru9I	TTAA	8 cut(s)	621, 903, 1017, 1563, 1970, 2144, 2442, 2672
TscAI	CASTG	9 cut(s)	420, 601, 850, 1041, 1112, 1237, 1330, 1525, 1550
TseFI	GTSAC	3 cut(s)	590, 601, 1703
TseI	GCWGC	3 cut(s)	126, 1362, 1460
Tsp45I	GTSAC	3 cut(s)	590, 601, 1703
TspGWI	ACGGA	3 cut(s)	242, 2537, 2553
TspRI	CASTG	9 cut(s)	420, 601, 850, 1041, 1112, 1237, 1330, 1525, 1550
VpaK11BI	GGWCC	2 cut(s)	725, 1933
VspI	ATTAAT	2 cut(s)	1970, 2144
XapI	RAATTY	4 cut(s)	215, 899, 1603, 2195
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2033
XmiI	GTMKAC	2 cut(s)	192, 1673
XspI	CTAG	5 cut(s)	813, 1026, 2574, 2586, 2634
ZrmI	AGTACT	1 cut(s)	1718
Zsp2I	ATGCAT	1 cut(s)	1747

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.