Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0003672

Physical Location & Seq

Genomic Coordinates Reverse (-)

31046351 .. 31055377

Gene Structure 9027 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1212700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2823 bp

ATGAACTCAAAATTTTCAAATACTTCCTCGAGTTCTTTCTTCGTAGCACTCCTCTGTCCTCCCCCACGACAAAACTCACAGTACCTCCTTATCCCAAAACCTGTCTCCCTCCGTGCAGCAGCAGCACTCCTCTCCATGACTTCCCTATTGCTTTCAACCAAGTTTTCTCTCTACCCAAACGGCACATTTCTCCATTTTATGTTCCCAAACAGACCTCAAAGAATCACCTTCCCCAGGAAAACACCGATAGCGTGCTCAGCCACAGAGCAGCCGAAGCGACGACAAAAACCGCCGAAGAAGAAGAAAAGCGTCGCTGACGGTGAGAAAGGGGTCGACCCAGTTGGCTTCCTTACCAAACATGGCATTTCCCATAAGCAATTTGCTCAGTTCCTCCGTGAAAGACACAAATCCGTGAAGGACCTCACTGATGAAATTTTCAATCGTCACGCCAATCTCCGGGATTTGGCTTCTGGGTTTGAGATATTGGGTATGCATCGACACCCGGAACACAGAGAGGACTATATGGAGTGGGCTCCAGGTGCTCGCTACTGTGCACTGGTTGGTGATTTCAACGGGTGGTCCCCTACTGAGAACTGTGCAAGAGAGGGTCATTTTGGCCACGATGACTATGGATACTGGTTCATCATTCTTGAAGATAAGTTGAGAGATGGAGAAAAACCAGATGAGCTCTATTTTCAACAGTATAACTACATCGATGACTATGATAAAGGTGACAGTGGTGTTACCATTGAGGAACTCTTTAAGAAAGCAAATGATGAGTATTGGGAACCTGGAGAAGACAGGTTTATAAAGAACCGTTTTGAAATGCCAGCAAAGTTGTATGAGCAGATTTTTGGGCCTAATGGTCCTCAAACATTGGAGGAGCTTGAAGAAATACCAGATGCAGAAACTAGATATAAATCTTGGAAAGAACAGCATAAAGATGACATACCTAGTAACGCACCTTGTTATGATGTGATTGACAATGGAAAAGAGTACGACATTTTCAATGTCGTGCTTGATCCTGTGTCGCAAGAAAAATTTCGAGGAAAGAAGCCTCCCTTGGCATATTGGTTTGAGACACGCAAAGGAAGGAAGGCATGGTTGAAAAAGTACAGTCCATGCATTCCTCATGGGAGCAAGTACAGGGTCTATTTTAACACTCCTAGTGGGCCATTGGAACGAGTACCTGCTTGGGCTACTTATGTGCAACCAGAGGCAGAAGGAGAGCAAGCTTTTGCAATACACTGGGAGCCACCGCCAGAGTATGCATTCAAATGGAAAAATTCACGCCCAAAAGTGCCAAAGTCTTTGCGCATTTATGAATGCCATGTTGGCATAAGTGGATCAGAGCCAAAAATGACTTCTTTCAATGATTTCACAGAGAAGGTCCTTCCTCATGTAAAGGAGGCCGGGTATAATACGATCCAACTGATGGGAGTTATTGAGCACAAAGATTATTTTACTGTTGGCTATAGAGTCACGAATTTATATGCTGTTAGCAGCCGATATGGAACTCCTGATGATTTCAAGCGGTTGGTTGATGAAGCACATGGACTAGGACTGCTGGTTTTTTTAGACATAGTACATTCCTACTCAGCTGCAGATGAGATGGTTGGATTGTCACTATTTGACGGAACTAATGACTGCTACTTCCACGCAGGTAAACGGGGACAACACAAGTACTGGGGTACCAGAATGTTTAAATATGGTGATCTGGATGTCCTGCATTTTCTTCTCTCAAATTTGAACTGGTGGGCCACAGAGTACCAAATTGATGGTTTTAATTTCCATTCACTCTCATCAATGATGTACACACACAATGGTTTTGCTTCTTTTACCGGTGACTTGGAGGAGTACTGTAATCAATATGTAGACAAGGACGCATTGCTATACCTCATTTTGGCAAATGAGATACTTCATGTTCTCCATCCGAATATAATAACAATTGCTGAAGATGCAACCTTCTATCCTGGGCTATGTGAACCCATCTCTCAAGGCGGGTTGGGATTTGATTATTGTGTCAATCTTTCTGTATCAGAGACGTGGTCAAATTTCCTTGAGAATGTTCCGGACCATGATTGGAGCATGAGTAAGATTGTCGGCACATTAATGGGCAACAGAAACTATGCTGATAAAATGCTTGTGTATGCTGAAAATCACAACCAGTCAATATCTGGAGGGCAGTCATTTGCGGAGATATTGTTTGGTGAAATCAAGGATGGTTCTCAAGACATGGATAAATTACTGCTCAGAGGTTGTTCCTTGCACAAGATGATCAGGTTGATTACTTTGACAATCAGTGGCCGTGCTTATCTCAACTTCATGGGCAATGAATTTGGGCATCCAGAGTGTGTTGAGTTCCCCATGTCAAGCAACAATTTCTCGTTTGCTCTTGCCAACCGTAGGTGGGATCTTCTTGCCAAGGAAGGATTGCATCGGAACTTGTTTATCTTTGATAAGGACTTGATGAAGTTGGATGAAACTGAAAGAGTACTTGCGAGAGTTTTACCGAGCATTCACCATGTGAATGACAATACCATGGTAATAGCTTACATGAGAGGCACCCTTCTATTTGTATTCAACTTTCACCCAACAGATTCTTATGAAGGATATGGAATAGGTGTAGAAGAGGCTGGAGAGTATCAACTCGTGCTAAATACGGATGAAATAAAGTATGGAGGTCAGGGACTTATAAAGGATGATCAATATTCTAGGAAAACTATTAGCAGAAGAGTCGATGGCCTCCGAAACTGCTTAGAAGTGCCTCTGCCTAGTAGGACTGCCCAGGTTTACAAACTAAGCCGGATCTTGAGAATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0007275 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009791 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0048856 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

941

Amino Acids

108.06

Weight (kDa)

6.1

Isoelectric Point (pI)

36.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Alpha-amylase	PF00128	465 - 534	6e-08	Alpha amylase, catalytic domain
Alpha-amylase_C	PF02806	842 - 934	8.5e-20	Alpha amylase, C-terminal all-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003672)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20440 AT3G20440 AT3G20440
fragaria_vesca	FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220
malus_domestica	MD11G1212700.v1.1
prunus_persica	Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1
pyrus_communis	pycom11g18770
rosa_chinensis	RchiOBHm_Chr5g0040901 RchiOBHm_Chr5g0040911
rosa_laevigata	RLG00000034012
rosa_multiflora	Rmu_sc0002848.1_g000016 Rmu_ssc0000416.1_g000003
rosa_roxburghii	Rroxscaffold_1G00039790
rosa_rugosa	Rorug05G0188500 Rorug05G0188600 Rorug05G0188600
rosa_samantha	Rh5AG274100 Rh5BG279100 Rh5CG311200 Rh5DG287300
rosa_wichuraiana	Rw5G025750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	809, 2696
Acc16I	TGCGCA	1 cut(s)	1316
Acc36I	ACCTGC	2 cut(s)	1198, 1652
Acc65I	GGTACC	1 cut(s)	1691
AccB1I	GGYRCC	2 cut(s)	1691, 2564
AccB7I	CCANNNNNTGG	1 cut(s)	1435
AccI	GTMKAC	2 cut(s)	333, 1875
AccIII	TCCGGA	1 cut(s)	2071
AciI	CCGC	5 cut(s)	290, 1259, 1534, 2001, 2195
AclWI	GGATC	5 cut(s)	1016, 1354, 1420, 2421, 2816
AcoI	YGGCCR	2 cut(s)	616, 2305
AcsI	RAATTY	8 cut(s)	11, 432, 1040, 1285, 1486, 1744, 2053, 2336
AcuI	CTGAAG	1 cut(s)	1974
AdeI	CACNNNGTG	1 cut(s)	2527
AfiI	CCNNNNNNNGG	7 cut(s)	234, 456, 463, 1435, 1903, 2406, 2410
AgeI	ACCGGT	1 cut(s)	1841
AjiI	CACGTC	1 cut(s)	2046
AjnI	CCWGG	5 cut(s)	233, 535, 790, 1972, 2787
AjuI	GAANNNNNNNTTGG	6 cut(s)	1046, 1078, 1317, 1348, 1349, 1380
AluBI	AGCT	5 cut(s)	688, 886, 1235, 1601, 2552
AluI	AGCT	5 cut(s)	688, 886, 1235, 1601, 2552
Alw21I	GWGCWC	5 cut(s)	257, 544, 556, 690, 1452
Alw26I	GTCTC	3 cut(s)	109, 1073, 2036
Alw44I	GTGCAC	1 cut(s)	552
AlwI	GGATC	5 cut(s)	1016, 1354, 1420, 2421, 2816
Ama87I	CYCGRG	1 cut(s)	28
Aor13HI	TCCGGA	1 cut(s)	2071
AoxI	GGCC	7 cut(s)	616, 857, 1172, 1410, 1758, 2305, 2743
ApaLI	GTGCAC	1 cut(s)	552
ApeKI	GCWGC	6 cut(s)	116, 119, 122, 268, 1503, 1601
ApoI	RAATTY	8 cut(s)	11, 432, 1040, 1285, 1486, 1744, 2053, 2336
AseI	ATTAAT	1 cut(s)	2111
AsiGI	ACCGGT	1 cut(s)	1841
Asp700I	GAANNNNTTC	1 cut(s)	1041
Asp718I	GGTACC	1 cut(s)	1691
AspLEI	GCGC	1 cut(s)	1317
AspS9I	GGNCC	8 cut(s)	418, 579, 857, 866, 1172, 1390, 1758, 2074
AsuC2I	CCSGG	3 cut(s)	458, 503, 1414
AsuHPI	GGTGA	9 cut(s)	217, 332, 575, 743, 1724, 1856, 2222, 2513, 2582
AvaI	CYCGRG	1 cut(s)	28
AvaII	GGWCC	5 cut(s)	418, 579, 866, 1390, 2074
BaeGI	GKGCMC	1 cut(s)	556
BaeI	ACNNNNGTAYC	2 cut(s)	1675, 1708
BalI	TGGCCA	1 cut(s)	618
BanI	GGYRCC	2 cut(s)	1691, 2564
BanII	GRGCYC	2 cut(s)	535, 690
BauI	CACGAG	1 cut(s)	2651
BbsI	GAAGAC	1 cut(s)	804
Bbv12I	GWGCWC	5 cut(s)	257, 544, 556, 690, 1452
BbvI	GCAGC	6 cut(s)	128, 131, 134, 280, 1515, 1588
BccI	CCATC	8 cut(s)	662, 1429, 1606, 1772, 1938, 1997, 2216, 2735
BceAI	ACGGC	2 cut(s)	196, 2292
BcgI	CGANNNNNNTGC	2 cut(s)	2719, 2753
BciT130I	CCWGG	5 cut(s)	235, 537, 792, 1974, 2789
BciVI	GTATCC	1 cut(s)	626
BclI	TGATCA	2 cut(s)	2277, 2704
BcnI	CCSGG	3 cut(s)	458, 503, 1414
BcoDI	GTCTC	3 cut(s)	109, 1073, 2036
BfaI	CTAG	6 cut(s)	912, 954, 1167, 1559, 2715, 2775
BfmI	CTRYAG	2 cut(s)	1474, 1602
BfuAI	ACCTGC	2 cut(s)	1198, 1652
BfuI	GTATCC	1 cut(s)	626
BglI	GCCNNNNNGGC	1 cut(s)	1335
BisI	GCNGC	6 cut(s)	117, 120, 123, 269, 1504, 1602
BlpI	GCTNAGC	1 cut(s)	256
BlsI	GCNGC	6 cut(s)	118, 121, 124, 270, 1505, 1603
BmcAI	AGTACT	3 cut(s)	1685, 1859, 2496
Bme1390I	CCNGG	8 cut(s)	235, 458, 503, 537, 792, 1414, 1974, 2789
Bme18I	GGWCC	5 cut(s)	418, 579, 866, 1390, 2074
BmeT110I	CYCGRG	1 cut(s)	28
BmgBI	CACGTC	1 cut(s)	2046
BmgT120I	GGNCC	8 cut(s)	418, 579, 857, 866, 1172, 1390, 1758, 2074
BmiI	GGNNCC	6 cut(s)	534, 581, 789, 1254, 1693, 2566
BmrFI	CCNGG	8 cut(s)	235, 458, 503, 537, 792, 1414, 1974, 2789
BmrI	ACTGGG	3 cut(s)	332, 1258, 1696
BmsI	GCATC	5 cut(s)	502, 892, 1948, 2353, 2446
BmuI	ACTGGG	3 cut(s)	332, 1258, 1696
BpiI	GAAGAC	1 cut(s)	804
BpmI	CTGGAG	4 cut(s)	519, 813, 2199, 2658
Bpu1102I	GCTNAGC	1 cut(s)	256
BpuEI	CTTGAG	3 cut(s)	1980, 2081, 2214
BpuMI	CCSGG	3 cut(s)	458, 503, 1414
Bsa29I	ATCGAT	1 cut(s)	714
BsaBI	GATNNNNATC	1 cut(s)	919
BsaJI	CCNNGG	6 cut(s)	233, 1062, 1973, 2424, 2541, 2787
BsaWI	WCCGGW	2 cut(s)	1841, 2071
BsaXI	ACNNNNNCTCC	8 cut(s)	69, 99, 1400, 1430, 2077, 2107, 2189, 2219
Bsc4I	CCNNNNNNNGG	7 cut(s)	234, 456, 463, 1435, 1903, 2406, 2410
Bse118I	RCCGGY	1 cut(s)	1841
Bse1I	ACTGG	7 cut(s)	338, 561, 641, 1253, 1691, 1757, 2167
Bse3DI	GCAATG	2 cut(s)	1886, 2338
Bse8I	GATNNNNATC	1 cut(s)	919
BseAI	TCCGGA	1 cut(s)	2071
BseBI	CCWGG	5 cut(s)	235, 537, 792, 1974, 2789
BseCI	ATCGAT	1 cut(s)	714
BseDI	CCNNGG	6 cut(s)	233, 1062, 1973, 2424, 2541, 2787
BseGI	GGATG	7 cut(s)	1726, 1930, 2227, 2344, 2485, 2671, 2707
BseJI	GATNNNNATC	1 cut(s)	919
BseLI	CCNNNNNNNGG	7 cut(s)	234, 456, 463, 1435, 1903, 2406, 2410
BseMI	GCAATG	2 cut(s)	1886, 2338
BseMII	CTCAG	5 cut(s)	270, 398, 579, 1611, 2266
BseNI	ACTGG	7 cut(s)	338, 561, 641, 1253, 1691, 1757, 2167
BseRI	GAGGAG	4 cut(s)	41, 119, 896, 1868
BseSI	GKGCMC	1 cut(s)	556
BseXI	GCAGC	6 cut(s)	128, 131, 134, 280, 1515, 1588
BsgI	GTGCAG	1 cut(s)	135
BshFI	GGCC	7 cut(s)	618, 859, 1174, 1412, 1760, 2307, 2745
BshNI	GGYRCC	2 cut(s)	1691, 2564
BshTI	ACCGGT	1 cut(s)	1841
BshVI	ATCGAT	1 cut(s)	714
BsiHKAI	GWGCWC	5 cut(s)	257, 544, 556, 690, 1452
BsiHKCI	CYCGRG	1 cut(s)	28
BsiSI	CCGG	6 cut(s)	457, 503, 1413, 1842, 2072, 2806
BslFI	GGGAC	3 cut(s)	565, 1686, 2703
BslI	CCNNNNNNNGG	7 cut(s)	234, 456, 463, 1435, 1903, 2406, 2410
BsmAI	GTCTC	3 cut(s)	109, 1073, 2036
BsmBI	CGTCTC	1 cut(s)	2036
BsmFI	GGGAC	3 cut(s)	565, 1686, 2703
BsmI	GAATGC	4 cut(s)	1125, 1271, 1331, 2517
BsnI	GGCC	7 cut(s)	618, 859, 1174, 1412, 1760, 2307, 2745
BsoBI	CYCGRG	1 cut(s)	28
Bsp1286I	GDGCHC	6 cut(s)	257, 535, 544, 556, 690, 1452
Bsp13I	TCCGGA	1 cut(s)	2071
Bsp1407I	TGTACA	1 cut(s)	1812
Bsp143I	GATC	8 cut(s)	1021, 1346, 1425, 1714, 2277, 2413, 2704, 2808
Bsp1720I	GCTNAGC	1 cut(s)	256
Bsp19I	CCATGG	1 cut(s)	2541
BspACI	CCGC	5 cut(s)	290, 1259, 1534, 2001, 2195
BspANI	GGCC	7 cut(s)	618, 859, 1174, 1412, 1760, 2307, 2745
BspCNI	CTCAG	5 cut(s)	269, 397, 580, 1610, 2265
BspDI	ATCGAT	1 cut(s)	714
BspEI	TCCGGA	1 cut(s)	2071
BspLI	GGNNCC	6 cut(s)	534, 581, 789, 1254, 1693, 2566
BspMAI	CTGCAG	1 cut(s)	1606
BspMI	ACCTGC	2 cut(s)	1198, 1652
BspPI	GGATC	5 cut(s)	1016, 1354, 1420, 2421, 2816
BspT107I	GGYRCC	2 cut(s)	1691, 2564
BsrDI	GCAATG	2 cut(s)	1886, 2338
BsrFI	RCCGGY	1 cut(s)	1841
BsrGI	TGTACA	1 cut(s)	1812
BsrI	ACTGG	7 cut(s)	338, 561, 641, 1253, 1691, 1757, 2167
BssAI	RCCGGY	1 cut(s)	1841
BssECI	CCNNGG	6 cut(s)	233, 1062, 1973, 2424, 2541, 2787
BssMI	GATC	8 cut(s)	1021, 1346, 1425, 1714, 2277, 2413, 2704, 2808
BssSI	CACGAG	1 cut(s)	2651
BssT1I	CCWWGG	3 cut(s)	1062, 2424, 2541
Bst2BI	CACGAG	1 cut(s)	2651
Bst2UI	CCWGG	5 cut(s)	235, 537, 792, 1974, 2789
Bst6I	CTCTTC	2 cut(s)	2625, 2728
BstAUI	TGTACA	1 cut(s)	1812
BstC8I	GCNNGC	4 cut(s)	253, 544, 831, 1233
BstDEI	CTNAG	7 cut(s)	256, 384, 588, 1597, 2252, 2758, 2801
BstDSI	CCRYGG	1 cut(s)	2541
BstENI	CCTNNNNNAGG	1 cut(s)	232
BstF5I	GGATG	7 cut(s)	1726, 1930, 2227, 2344, 2485, 2671, 2707
BstHHI	GCGC	1 cut(s)	1317
BstKTI	GATC	8 cut(s)	1024, 1349, 1428, 1717, 2280, 2416, 2707, 2811
BstMAI	GTCTC	3 cut(s)	109, 1073, 2036
BstMBI	GATC	8 cut(s)	1021, 1346, 1425, 1714, 2277, 2413, 2704, 2808
BstMWI	GCNNNNNNNGC	6 cut(s)	122, 257, 274, 539, 1335, 1958
BstNI	CCWGG	5 cut(s)	235, 537, 792, 1974, 2789
BstSCI	CCNGG	8 cut(s)	233, 456, 501, 535, 790, 1412, 1972, 2787
BstSFI	CTRYAG	2 cut(s)	1474, 1602
BstSLI	GKGCMC	1 cut(s)	556
BstV1I	GCAGC	6 cut(s)	128, 131, 134, 280, 1515, 1588
BstV2I	GAAGAC	1 cut(s)	804
BstX2I	RGATCY	2 cut(s)	2413, 2808
BstXI	CCANNNNNNTGG	1 cut(s)	1778
BstYI	RGATCY	2 cut(s)	2413, 2808
Bsu15I	ATCGAT	1 cut(s)	714
BsuI	GTATCC	1 cut(s)	626
BsuRI	GGCC	7 cut(s)	618, 859, 1174, 1412, 1760, 2307, 2745
BsuTUI	ATCGAT	1 cut(s)	714
BtgI	CCRYGG	1 cut(s)	2541
BtrI	CACGTC	1 cut(s)	2046
BtsCI	GGATG	7 cut(s)	1726, 1930, 2227, 2344, 2485, 2671, 2707
BtsIMutI	CAGTG	5 cut(s)	423, 554, 742, 1246, 2308
BveI	ACCTGC	2 cut(s)	1198, 1652
Cac8I	GCNNGC	4 cut(s)	253, 544, 831, 1233
CfoI	GCGC	1 cut(s)	1317
Cfr10I	RCCGGY	1 cut(s)	1841
Cfr13I	GGNCC	8 cut(s)	418, 579, 857, 866, 1172, 1390, 1758, 2074
ClaI	ATCGAT	1 cut(s)	714
CseI	GACGC	2 cut(s)	298, 1892
CspAI	ACCGGT	1 cut(s)	1841
DdeI	CTNAG	7 cut(s)	256, 384, 588, 1597, 2252, 2758, 2801
DpnI	GATC	8 cut(s)	1023, 1348, 1427, 1716, 2279, 2415, 2706, 2810
DpnII	GATC	8 cut(s)	1021, 1346, 1425, 1714, 2277, 2413, 2704, 2808
DraI	TTTAAA	1 cut(s)	1705
DraIII	CACNNNGTG	1 cut(s)	2527
EaeI	YGGCCR	2 cut(s)	616, 2305
Eam1104I	CTCTTC	2 cut(s)	2625, 2728
EarI	CTCTTC	2 cut(s)	2625, 2728
Ecl136II	GAGCTC	1 cut(s)	688
Eco130I	CCWWGG	3 cut(s)	1062, 2424, 2541
Eco24I	GRGCYC	2 cut(s)	535, 690
Eco47I	GGWCC	5 cut(s)	418, 579, 866, 1390, 2074
Eco53kI	GAGCTC	1 cut(s)	688
Eco57I	CTGAAG	1 cut(s)	1974
Eco88I	CYCGRG	1 cut(s)	28
EcoICRI	GAGCTC	1 cut(s)	688
EcoNI	CCTNNNNNAGG	1 cut(s)	232
EcoO109I	RGGNCCY	2 cut(s)	418, 1390
EcoRII	CCWGG	5 cut(s)	233, 535, 790, 1972, 2787
EcoT14I	CCWWGG	3 cut(s)	1062, 2424, 2541
EcoT22I	ATGCAT	3 cut(s)	495, 1127, 1273
EcoT38I	GRGCYC	2 cut(s)	535, 690
ErhI	CCWWGG	3 cut(s)	1062, 2424, 2541
Esp3I	CGTCTC	1 cut(s)	2036
FalI	AAGNNNNNCTT	2 cut(s)	1046, 1078
FaqI	GGGAC	3 cut(s)	565, 1686, 2703
FauI	CCCGC	1 cut(s)	1994
FbaI	TGATCA	2 cut(s)	2277, 2704
FblI	GTMKAC	2 cut(s)	333, 1875
Fnu4HI	GCNGC	6 cut(s)	117, 120, 123, 269, 1504, 1602
FokI	GGATG	7 cut(s)	1733, 1917, 2234, 2331, 2492, 2678, 2714
FriOI	GRGCYC	2 cut(s)	535, 690
Fsp4HI	GCNGC	6 cut(s)	117, 120, 123, 269, 1504, 1602
FspBI	CTAG	6 cut(s)	912, 954, 1167, 1559, 2715, 2775
FspI	TGCGCA	1 cut(s)	1316
GlaI	GCGC	1 cut(s)	1316
GluI	GCNGC	6 cut(s)	117, 120, 123, 269, 1504, 1602
GsuI	CTGGAG	4 cut(s)	519, 813, 2199, 2658
HaeIII	GGCC	7 cut(s)	618, 859, 1174, 1412, 1760, 2307, 2745
HapII	CCGG	6 cut(s)	457, 503, 1413, 1842, 2072, 2806
HgaI	GACGC	2 cut(s)	298, 1892
HhaI	GCGC	1 cut(s)	1317
Hin6I	GCGC	1 cut(s)	1315
HinP1I	GCGC	1 cut(s)	1315
HincII	GTYRAC	1 cut(s)	334
HindII	GTYRAC	1 cut(s)	334
HindIII	AAGCTT	1 cut(s)	1233
HinfI	GANTC	4 cut(s)	222, 1479, 2600, 2736
HpaII	CCGG	6 cut(s)	457, 503, 1413, 1842, 2072, 2806
HphI	GGTGA	9 cut(s)	217, 332, 575, 743, 1724, 1856, 2222, 2513, 2582
Hpy166II	GTNNAC	7 cut(s)	334, 554, 1667, 1815, 1876, 1985, 2794
Hpy188I	TCNGA	6 cut(s)	1351, 1935, 2041, 2255, 2442, 2750
Hpy188III	TCNNGA	9 cut(s)	650, 1483, 1520, 1718, 2072, 2178, 2231, 2348, 2812
Hpy8I	GTNNAC	7 cut(s)	334, 554, 1667, 1815, 1876, 1985, 2794
Hpy99I	CGWCG	2 cut(s)	282, 314
HpyCH4IV	ACGT	1 cut(s)	2045
HpyF10VI	GCNNNNNNNGC	6 cut(s)	122, 257, 274, 539, 1335, 1958
HpyF3I	CTNAG	7 cut(s)	256, 384, 588, 1597, 2252, 2758, 2801
HpySE526I	ACGT	1 cut(s)	2045
HspAI	GCGC	1 cut(s)	1315
Kpn2I	TCCGGA	1 cut(s)	2071
KpnI	GGTACC	1 cut(s)	1695
Ksp22I	TGATCA	2 cut(s)	2277, 2704
Kzo9I	GATC	8 cut(s)	1021, 1346, 1425, 1714, 2277, 2413, 2704, 2808
LmnI	GCTCC	5 cut(s)	538, 883, 1137, 1252, 2085
Lsp1109I	GCAGC	6 cut(s)	128, 131, 134, 280, 1515, 1588
LweI	GCATC	5 cut(s)	502, 892, 1948, 2353, 2446
MaeI	CTAG	6 cut(s)	912, 954, 1167, 1559, 2715, 2775
MaeII	ACGT	1 cut(s)	2045
MaeIII	GTNAC	7 cut(s)	443, 731, 742, 956, 1480, 1623, 1844
MalI	GATC	8 cut(s)	1023, 1348, 1427, 1716, 2279, 2415, 2706, 2810
MboI	GATC	8 cut(s)	1021, 1346, 1425, 1714, 2277, 2413, 2704, 2808
MfeI	CAATTG	1 cut(s)	1947
MflI	RGATCY	2 cut(s)	2413, 2808
MhlI	GDGCHC	6 cut(s)	257, 535, 544, 556, 690, 1452
MlsI	TGGCCA	1 cut(s)	618
MluNI	TGGCCA	1 cut(s)	618
MlyI	GAGTC	2 cut(s)	1488, 2745
MmeI	TCCRAC	3 cut(s)	1453, 1597, 2457
Mox20I	TGGCCA	1 cut(s)	618
Mph1103I	ATGCAT	3 cut(s)	495, 1127, 1273
MroI	TCCGGA	1 cut(s)	2071
MroXI	GAANNNNTTC	1 cut(s)	1041
MscI	TGGCCA	1 cut(s)	618
MseI	TTAA	5 cut(s)	762, 1158, 1704, 1785, 2111
MslI	CAYNNNNRTG	4 cut(s)	942, 1276, 2111, 2529
Msp20I	TGGCCA	1 cut(s)	618
MspA1I	CMGCKG	1 cut(s)	1601
MspI	CCGG	6 cut(s)	457, 503, 1413, 1842, 2072, 2806
MspR9I	CCNGG	8 cut(s)	235, 458, 503, 537, 792, 1414, 1974, 2789
MunI	CAATTG	1 cut(s)	1947
Mva1269I	GAATGC	4 cut(s)	1125, 1271, 1331, 2517
MvaI	CCWGG	5 cut(s)	235, 537, 792, 1974, 2789
MwoI	GCNNNNNNNGC	6 cut(s)	122, 257, 274, 539, 1335, 1958
NciI	CCSGG	3 cut(s)	458, 503, 1414
NcoI	CCATGG	1 cut(s)	2541
NdeII	GATC	8 cut(s)	1021, 1346, 1425, 1714, 2277, 2413, 2704, 2808
NlaIV	GGNNCC	6 cut(s)	534, 581, 789, 1254, 1693, 2566
NmuCI	GTSAC	5 cut(s)	443, 731, 1480, 1623, 1844
NsbI	TGCGCA	1 cut(s)	1316
NsiI	ATGCAT	3 cut(s)	495, 1127, 1273
PaeR7I	CTCGAG	1 cut(s)	28
PctI	GAATGC	4 cut(s)	1125, 1271, 1331, 2517
PdmI	GAANNNNTTC	1 cut(s)	1041
PfeI	GAWTC	2 cut(s)	222, 2600
PflFI	GACNNNGTC	1 cut(s)	2047
PflMI	CCANNNNNTGG	1 cut(s)	1435
PfoI	TCCNGGA	1 cut(s)	456
PinAI	ACCGGT	1 cut(s)	1841
PkrI	GCNGC	6 cut(s)	118, 121, 124, 270, 1505, 1603
PleI	GAGTC	2 cut(s)	1487, 2744
PpsI	GAGTC	2 cut(s)	1487, 2744
PpuMI	RGGWCCY	2 cut(s)	418, 1390
PshBI	ATTAAT	1 cut(s)	2111
PsiI	TTATAA	2 cut(s)	809, 2696
Psp124BI	GAGCTC	1 cut(s)	690
Psp5II	RGGWCCY	2 cut(s)	418, 1390
Psp6I	CCWGG	5 cut(s)	233, 535, 790, 1972, 2787
PspGI	CCWGG	5 cut(s)	233, 535, 790, 1972, 2787
PspN4I	GGNNCC	6 cut(s)	534, 581, 789, 1254, 1693, 2566
PspPI	GGNCC	8 cut(s)	418, 579, 857, 866, 1172, 1390, 1758, 2074
PspPPI	RGGWCCY	2 cut(s)	418, 1390
PspXI	VCTCGAGB	1 cut(s)	28
PstI	CTGCAG	1 cut(s)	1606
PsuI	RGATCY	2 cut(s)	2413, 2808
PsyI	GACNNNGTC	1 cut(s)	2047
PvuII	CAGCTG	1 cut(s)	1601
RseI	CAYNNNNRTG	4 cut(s)	942, 1276, 2111, 2529
SacI	GAGCTC	1 cut(s)	690
SalI	GTCGAC	1 cut(s)	332
SaqAI	TTAA	5 cut(s)	762, 1158, 1704, 1785, 2111
SatI	GCNGC	6 cut(s)	117, 120, 123, 269, 1504, 1602
Sau3AI	GATC	8 cut(s)	1021, 1346, 1425, 1714, 2277, 2413, 2704, 2808
Sau96I	GGNCC	8 cut(s)	418, 579, 857, 866, 1172, 1390, 1758, 2074
ScaI	AGTACT	3 cut(s)	1685, 1859, 2496
SchI	GAGTC	2 cut(s)	1488, 2745
ScrFI	CCNGG	8 cut(s)	235, 458, 503, 537, 792, 1414, 1974, 2789
SduI	GDGCHC	6 cut(s)	257, 535, 544, 556, 690, 1452
SfaNI	GCATC	5 cut(s)	502, 892, 1948, 2353, 2446
SfcI	CTRYAG	2 cut(s)	1474, 1602
Sfr274I	CTCGAG	1 cut(s)	28
SinI	GGWCC	5 cut(s)	418, 579, 866, 1390, 2074
SlaI	CTCGAG	1 cut(s)	28
SmiMI	CAYNNNNRTG	4 cut(s)	942, 1276, 2111, 2529
SmlI	CTYRAG	5 cut(s)	28, 1995, 2060, 2229, 2812
SmoI	CTYRAG	5 cut(s)	28, 1995, 2060, 2229, 2812
SsiI	CCGC	5 cut(s)	290, 1259, 1534, 2001, 2195
SspI	AATATT	1 cut(s)	2711
SspMI	CTAG	6 cut(s)	912, 954, 1167, 1559, 2715, 2775
SstI	GAGCTC	1 cut(s)	690
StyD4I	CCNGG	8 cut(s)	233, 456, 501, 535, 790, 1412, 1972, 2787
StyI	CCWWGG	3 cut(s)	1062, 2424, 2541
TaiI	ACGT	1 cut(s)	2048
TaqI	TCGA	6 cut(s)	29, 333, 496, 714, 1045, 2739
TatI	WGTACW	7 cut(s)	1113, 1143, 1585, 1683, 1812, 1857, 2494
TfiI	GAWTC	2 cut(s)	222, 2600
Tru1I	TTAA	5 cut(s)	762, 1158, 1704, 1785, 2111
Tru9I	TTAA	5 cut(s)	762, 1158, 1704, 1785, 2111
TscAI	CASTG	5 cut(s)	430, 561, 742, 1253, 2308
TseFI	GTSAC	5 cut(s)	443, 731, 1480, 1623, 1844
TseI	GCWGC	6 cut(s)	116, 119, 122, 268, 1503, 1601
Tsp45I	GTSAC	5 cut(s)	443, 731, 1480, 1623, 1844
TspGWI	ACGGA	5 cut(s)	101, 383, 400, 1650, 2678
TspRI	CASTG	5 cut(s)	430, 561, 742, 1253, 2308
Tth111I	GACNNNGTC	1 cut(s)	2047
Van91I	CCANNNNNTGG	1 cut(s)	1435
VneI	GTGCAC	1 cut(s)	552
VpaK11BI	GGWCC	5 cut(s)	418, 579, 866, 1390, 2074
VspI	ATTAAT	1 cut(s)	2111
XagI	CCTNNNNNAGG	1 cut(s)	232
XapI	RAATTY	8 cut(s)	11, 432, 1040, 1285, 1486, 1744, 2053, 2336
XcmI	CCANNNNNNNNNTGG	2 cut(s)	626, 2174
XhoI	CTCGAG	1 cut(s)	28
XmiI	GTMKAC	2 cut(s)	333, 1875
XmnI	GAANNNNTTC	1 cut(s)	1041
XspI	CTAG	6 cut(s)	912, 954, 1167, 1559, 2715, 2775
ZrmI	AGTACT	3 cut(s)	1685, 1859, 2496
Zsp2I	ATGCAT	3 cut(s)	495, 1127, 1273

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.