Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0003672

Physical Location & Seq

Genomic Coordinates Reverse (-)

57482436 .. 57490627

Gene Structure 8192 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00039790.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2175 bp

ATGCAGGTGCTCGCTACTGCGCACTGGTTGGTGATTTCAATGGGGTGGTCTCCCACAGAGAACTGTGCAAAAGAGGGTCATTTTGGACATGATGACTATGGATACTGGTTCATTATTCTTGAAGATAAGTTGAGGAATGGAGAGAAACCAGATGAGCTGTACTTTCAACAGTATAACTATGTAGATGATTACGATAAAGGTGACAGTGGTGTTACCATTGAAGAAGTCTTTAAGAAAGCAAATGATGAGTATTGGGAACCTGGAGAAGACCGGTTTCTAAAAAACCGCTTAGAAGTACCAGCAAAGTTGTATGAGCAGATTTTTGGGCCTAATGGACCTCAAACGTTAGAGGAACTTGAAGAAATACCAGATGCAGAAACAAGATATAAAGCATGGAAAGAACAGCATAAAGATGACCCGCCTAGTAACTTACCTTCTTATGATGTGATTGACAACGGAAAAGTGTATGACATTTACAATGTTGTACTTGATCCAACGCAGATGGAGGACAAGCTTTTTGCAATCCACTGGGAACCACCCCCAGAACTTGCATACAAATGGAAGAATGCACGCCCTAAAGTGCCAAAGTCCCTGCGCATTTATGAATGTCATGTTGGCATAAGTGGATCAGAGCCTAAAGTTCTTCCTCATGTAAAGGAGGCTGGCTATAATGCAATTCAGCTGATTGGAGTTATTGAGCACAAAGATTATTTCACTGTTGGTTATAGAGCCACTAATTTATATGCTGTTAGCAGCCGATTTGGCACTCCTGATGATTTCAAGCGGTTGGTTGATGAAGCGCATGGACAAGGACTGCTGGTCTTCTTGGAGATTGTCCATTCCTACTCAGCTGCAGATGAGATGGTTGGATTAGGGCTTTTTGATGGAACAAATGACTGCTACTTCCACACTGACTCTGATTACTCAACATCGAGACTGACTGCAGTGAGATTACTTGATAGAGAGTGGGTTGGATCTGATCAGGTGGCAGTAGCTAATCAATGTGGATTTATTCTCTTTAACAACATTGCCATTTGTTCTCATATTCATATATCATGTTGGGCCCTCCATTTCATGATATCAACAATATCTTCTCAAATAGGCAAACGGGGACATCACAAGCACTGGGGTACCAGAATGTTTAAATATGGTGATCTGGATGTTCTGCATTTCCTTCTCTCGAATTTGAACTGGTGGATCACGGAGTACCAAGTTGATGGTTTTCAGTTCCATTCACTCTCATCAATGATTTATACTCATAATGGTTTTGCTTCGTTTACCGGTGACTTGGAGGAGTACTGCAATCAATATGTTGATAGGGACGCATTGTTATACCTCATTTTGGCAAACGAAATACTTCATGCTCTCCACCCAGATATAATAACGATTGCTGAAGATGCAACCTTCTATCCAGGTCTATGTGAACCAATCTCTCAAGGCGGATTGGGCTTTGATTATTGTGTCAATCTTTCTGTCTCAGAGATGTGGTCATCTTTCCTTGAGAATGTTCCAGACCATGATTGGAGCATGATGGACATTCATTATGTTCAACCCACTCTGCAGATTGTGAGTACATTAATGGGCAACAAAAATTATGCAGATAAGATGCTCATTCAATTTCTGGAGGGAGATCTTTTTGCAGAGATACTGTTTGGTGAAATCAGGGAGGGTTCTCCTGACAAGGAGAAATTATTGCTCAGAGGTTGTTCCTTGCACAAGATGATCAGATTAATTACCATGACAATTGGTGGCCGCGCTTATCTCAACTTCATGGGCAATGAATTTGGGCATCCAGAGAGGGTTGAGTTCCCAATGCCAAGCAACAATTTCTCATTTTCTCTTGCCAACCGAAGGTGGGATCTTCTTGCCAATGAGGGATTGCATCTCAACTTGTTTACCTTCGATAAGGTAATTGCTTACATAAGAGGGCCTCTCTTCTATTCGTATTTAACTTTTCATCCAACAGATTCTTATGAAGGATACCGAATAGGTGTAGAAGAAGCTGGAGAGTATCAACTTGTACTAAATACGGATGAAGGGAAGTACGGAGGTCAAGGGCTTTTGAAGGATGACCAATATTCTAGGACAACTACTAGCCAAAGAAGTGATGGCCTCCGAAACAGCTTGGAAGTTCCTCTGCCTAGTAGGACTGCCCAGGTTTGTAAATTCCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0007275 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009791 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0048856 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

724

Amino Acids

83.04

Weight (kDa)

5.0

Isoelectric Point (pI)

32.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Alpha-amylase	PF00128	215 - 283	1.1e-06	Alpha amylase, catalytic domain
Alpha-amylase_C	PF02806	635 - 722	1.2e-14	Alpha amylase, C-terminal all-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003672)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20440 AT3G20440 AT3G20440
fragaria_vesca	FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220
malus_domestica	MD11G1212700.v1.1
prunus_persica	Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1
pyrus_communis	pycom11g18770
rosa_chinensis	RchiOBHm_Chr5g0040901 RchiOBHm_Chr5g0040911
rosa_laevigata	RLG00000034012
rosa_multiflora	Rmu_sc0002848.1_g000016 Rmu_ssc0000416.1_g000003
rosa_roxburghii	Rroxscaffold_1G00039790
rosa_rugosa	Rorug05G0188500 Rorug05G0188600 Rorug05G0188600
rosa_samantha	Rh5AG274100 Rh5BG279100 Rh5CG311200 Rh5DG287300
rosa_wichuraiana	Rw5G025750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	21, 596
Acc65I	GGTACC	1 cut(s)	1130
AccB1I	GGYRCC	1 cut(s)	1130
AccII	CGCG	1 cut(s)	1755
AciI	CCGC	5 cut(s)	286, 419, 784, 1440, 1753
AclI	AACGTT	1 cut(s)	344
AclWI	GGATC	5 cut(s)	485, 634, 982, 1205, 1866
AcoI	YGGCCR	1 cut(s)	1750
AcsI	RAATTY	3 cut(s)	1183, 1781, 2165
AcuI	CTGAAG	1 cut(s)	1413
AfaI	GTAC	9 cut(s)	161, 297, 486, 1132, 1208, 1298, 1573, 2022, 2045
AfiI	CCNNNNNNNGG	3 cut(s)	1342, 1851, 1855
AgeI	ACCGGT	2 cut(s)	270, 1280
AhdI	GACNNNNNGTC	1 cut(s)	818
AjnI	CCWGG	3 cut(s)	259, 1411, 2154
AjuI	GAANNNNNNNTTGG	2 cut(s)	597, 629
AluBI	AGCT	7 cut(s)	157, 514, 682, 851, 995, 2003, 2124
AluI	AGCT	7 cut(s)	157, 514, 682, 851, 995, 2003, 2124
Alw21I	GWGCWC	2 cut(s)	12, 702
Alw26I	GTCTC	3 cut(s)	54, 928, 1480
AlwI	GGATC	5 cut(s)	485, 634, 982, 1205, 1866
AoxI	GGCC	5 cut(s)	326, 1062, 1750, 1928, 2110
ApaI	GGGCCC	1 cut(s)	1066
ApeKI	GCWGC	2 cut(s)	753, 851
ApoI	RAATTY	3 cut(s)	1183, 1781, 2165
ArsI	GACNNNNNNTTYG	2 cut(s)	500, 532
AseI	ATTAAT	2 cut(s)	1577, 1730
AsiGI	ACCGGT	2 cut(s)	270, 1280
Asp700I	GAANNNNTTC	1 cut(s)	1356
Asp718I	GGTACC	1 cut(s)	1130
AspLEI	GCGC	4 cut(s)	22, 597, 802, 1757
AspS9I	GGNCC	5 cut(s)	326, 335, 1062, 1063, 1928
AsuHPI	GGTGA	5 cut(s)	43, 212, 1163, 1295, 1667
AvaII	GGWCC	1 cut(s)	335
BaeGI	GKGCMC	1 cut(s)	1066
BaeI	ACNNNNGTAYC	2 cut(s)	1114, 1147
BanI	GGYRCC	1 cut(s)	1130
BanII	GRGCYC	1 cut(s)	1066
BarI	GAAGNNNNNNTAC	2 cut(s)	418, 450
BbsI	GAAGAC	2 cut(s)	273, 814
Bbv12I	GWGCWC	2 cut(s)	12, 702
BbvI	GCAGC	2 cut(s)	765, 838
BccI	CCATC	6 cut(s)	496, 856, 878, 1211, 1525, 2102
BciT130I	CCWGG	3 cut(s)	261, 1413, 2156
BciVI	GTATCC	2 cut(s)	95, 1973
BclI	TGATCA	2 cut(s)	979, 1722
BcoDI	GTCTC	3 cut(s)	54, 928, 1480
BfaI	CTAG	4 cut(s)	423, 2082, 2094, 2142
BfmI	CTRYAG	3 cut(s)	852, 942, 1559
BfuI	GTATCC	2 cut(s)	95, 1973
BglI	GCCNNNNNGGC	1 cut(s)	762
BglII	AGATCT	1 cut(s)	1630
BisI	GCNGC	3 cut(s)	754, 852, 1753
BlsI	GCNGC	3 cut(s)	755, 853, 1754
BmcAI	AGTACT	1 cut(s)	1298
Bme1390I	CCNGG	3 cut(s)	261, 1413, 2156
Bme18I	GGWCC	1 cut(s)	335
BmeRI	GACNNNNNGTC	1 cut(s)	818
BmgT120I	GGNCC	5 cut(s)	326, 335, 1062, 1063, 1928
BmiI	GGNNCC	4 cut(s)	258, 534, 1064, 1132
BmrFI	CCNGG	3 cut(s)	261, 1413, 2156
BmrI	ACTGGG	2 cut(s)	538, 1135
BmsI	GCATC	5 cut(s)	361, 1387, 1596, 1798, 1891
BmuI	ACTGGG	2 cut(s)	538, 1135
BpiI	GAAGAC	2 cut(s)	273, 814
BplI	GAGNNNNNCTC	2 cut(s)	1917, 1949
BpmI	CTGGAG	3 cut(s)	282, 1643, 2025
BpuEI	CTTGAG	2 cut(s)	1419, 1520
BsaI	GGTCTC	1 cut(s)	54
BsaJI	CCNNGG	1 cut(s)	2154
BsaWI	WCCGGW	2 cut(s)	270, 1280
Bsc4I	CCNNNNNNNGG	3 cut(s)	1342, 1851, 1855
Bse118I	RCCGGY	2 cut(s)	270, 1280
Bse1I	ACTGG	5 cut(s)	29, 110, 533, 1130, 1196
Bse3DI	GCAATG	2 cut(s)	1026, 1783
BseBI	CCWGG	3 cut(s)	261, 1413, 2156
BseDI	CCNNGG	1 cut(s)	2154
BseGI	GGATG	5 cut(s)	1165, 1789, 1957, 2038, 2074
BseLI	CCNNNNNNNGG	3 cut(s)	1342, 1851, 1855
BseMI	GCAATG	2 cut(s)	1026, 1783
BseMII	CTCAG	3 cut(s)	861, 1491, 1711
BseNI	ACTGG	5 cut(s)	29, 110, 533, 1130, 1196
BseRI	GAGGAG	1 cut(s)	1307
BseSI	GKGCMC	1 cut(s)	1066
BseXI	GCAGC	2 cut(s)	765, 838
Bsh1236I	CGCG	1 cut(s)	1755
BshFI	GGCC	5 cut(s)	328, 1064, 1752, 1930, 2112
BshNI	GGYRCC	1 cut(s)	1130
BshTI	ACCGGT	2 cut(s)	270, 1280
BsiHKAI	GWGCWC	2 cut(s)	12, 702
BsiSI	CCGG	2 cut(s)	271, 1281
BslFI	GGGAC	3 cut(s)	574, 1125, 1334
BslI	CCNNNNNNNGG	3 cut(s)	1342, 1851, 1855
BsmAI	GTCTC	3 cut(s)	54, 928, 1480
BsmFI	GGGAC	3 cut(s)	574, 1125, 1334
BsmI	GAATGC	1 cut(s)	571
BsnI	GGCC	5 cut(s)	328, 1064, 1752, 1930, 2112
Bso31I	GGTCTC	1 cut(s)	54
Bsp120I	GGGCCC	1 cut(s)	1062
Bsp1286I	GDGCHC	3 cut(s)	12, 702, 1066
Bsp143I	GATC	9 cut(s)	490, 626, 974, 979, 1153, 1197, 1630, 1722, 1858
BspACI	CCGC	5 cut(s)	286, 419, 784, 1440, 1753
BspANI	GGCC	5 cut(s)	328, 1064, 1752, 1930, 2112
BspCNI	CTCAG	3 cut(s)	860, 1490, 1710
BspFNI	CGCG	1 cut(s)	1755
BspHI	TCATGA	1 cut(s)	1074
BspLI	GGNNCC	4 cut(s)	258, 534, 1064, 1132
BspMAI	CTGCAG	3 cut(s)	856, 946, 1563
BspPI	GGATC	5 cut(s)	485, 634, 982, 1205, 1866
BspT107I	GGYRCC	1 cut(s)	1130
BspTNI	GGTCTC	1 cut(s)	54
BsrDI	GCAATG	2 cut(s)	1026, 1783
BsrFI	RCCGGY	2 cut(s)	270, 1280
BsrI	ACTGG	5 cut(s)	29, 110, 533, 1130, 1196
BssAI	RCCGGY	2 cut(s)	270, 1280
BssECI	CCNNGG	1 cut(s)	2154
BssMI	GATC	9 cut(s)	490, 626, 974, 979, 1153, 1197, 1630, 1722, 1858
Bst2UI	CCWGG	3 cut(s)	261, 1413, 2156
Bst4CI	ACNGT	5 cut(s)	65, 171, 206, 718, 1650
Bst6I	CTCTTC	1 cut(s)	1940
BstC8I	GCNNGC	3 cut(s)	12, 571, 664
BstDEI	CTNAG	4 cut(s)	289, 847, 1477, 1697
BstF5I	GGATG	5 cut(s)	1165, 1789, 1957, 2038, 2074
BstFNI	CGCG	1 cut(s)	1755
BstHHI	GCGC	4 cut(s)	22, 597, 802, 1757
BstKTI	GATC	9 cut(s)	493, 629, 977, 982, 1156, 1200, 1633, 1725, 1861
BstMAI	GTCTC	3 cut(s)	54, 928, 1480
BstMBI	GATC	9 cut(s)	490, 626, 974, 979, 1153, 1197, 1630, 1722, 1858
BstMWI	GCNNNNNNNGC	3 cut(s)	762, 1397, 1446
BstNI	CCWGG	3 cut(s)	261, 1413, 2156
BstSCI	CCNGG	3 cut(s)	259, 1411, 2154
BstSFI	CTRYAG	3 cut(s)	852, 942, 1559
BstSLI	GKGCMC	1 cut(s)	1066
BstUI	CGCG	1 cut(s)	1755
BstV1I	GCAGC	2 cut(s)	765, 838
BstV2I	GAAGAC	2 cut(s)	273, 814
BstX2I	RGATCY	3 cut(s)	974, 1630, 1858
BstXI	CCANNNNNNTGG	1 cut(s)	1217
BstYI	RGATCY	3 cut(s)	974, 1630, 1858
BsuI	GTATCC	2 cut(s)	95, 1973
BsuRI	GGCC	5 cut(s)	328, 1064, 1752, 1930, 2112
BtsCI	GGATG	5 cut(s)	1165, 1789, 1957, 2038, 2074
BtsI	GCAGTG	1 cut(s)	951
BtsIMutI	CAGTG	7 cut(s)	22, 211, 526, 714, 909, 951, 1123
Cac8I	GCNNGC	3 cut(s)	12, 571, 664
CciI	TCATGA	1 cut(s)	1074
CfoI	GCGC	4 cut(s)	22, 597, 802, 1757
Cfr10I	RCCGGY	2 cut(s)	270, 1280
Cfr13I	GGNCC	5 cut(s)	326, 335, 1062, 1063, 1928
CseI	GACGC	1 cut(s)	1331
Csp6I	GTAC	9 cut(s)	160, 296, 485, 1131, 1207, 1297, 1572, 2021, 2044
CspAI	ACCGGT	2 cut(s)	270, 1280
CviQI	GTAC	9 cut(s)	160, 296, 485, 1131, 1207, 1297, 1572, 2021, 2044
DdeI	CTNAG	4 cut(s)	289, 847, 1477, 1697
DpnI	GATC	9 cut(s)	492, 628, 976, 981, 1155, 1199, 1632, 1724, 1860
DpnII	GATC	9 cut(s)	490, 626, 974, 979, 1153, 1197, 1630, 1722, 1858
DraI	TTTAAA	1 cut(s)	1144
DriI	GACNNNNNGTC	1 cut(s)	818
EaeI	YGGCCR	1 cut(s)	1750
Eam1104I	CTCTTC	1 cut(s)	1940
Eam1105I	GACNNNNNGTC	1 cut(s)	818
EarI	CTCTTC	1 cut(s)	1940
EciI	GGCGGA	1 cut(s)	1455
Eco24I	GRGCYC	1 cut(s)	1066
Eco31I	GGTCTC	1 cut(s)	54
Eco32I	GATATC	1 cut(s)	1080
Eco47I	GGWCC	1 cut(s)	335
Eco57I	CTGAAG	1 cut(s)	1413
EcoO109I	RGGNCCY	2 cut(s)	1063, 1928
EcoRII	CCWGG	3 cut(s)	259, 1411, 2154
EcoRV	GATATC	1 cut(s)	1080
EcoT38I	GRGCYC	1 cut(s)	1066
FaqI	GGGAC	3 cut(s)	574, 1125, 1334
FauI	CCCGC	1 cut(s)	426
FbaI	TGATCA	2 cut(s)	979, 1722
Fnu4HI	GCNGC	3 cut(s)	754, 852, 1753
FokI	GGATG	5 cut(s)	1172, 1776, 1944, 2045, 2081
FriOI	GRGCYC	1 cut(s)	1066
Fsp4HI	GCNGC	3 cut(s)	754, 852, 1753
FspBI	CTAG	4 cut(s)	423, 2082, 2094, 2142
FspI	TGCGCA	2 cut(s)	21, 596
GlaI	GCGC	4 cut(s)	21, 596, 801, 1756
GluI	GCNGC	3 cut(s)	754, 852, 1753
GsuI	CTGGAG	3 cut(s)	282, 1643, 2025
HaeIII	GGCC	5 cut(s)	328, 1064, 1752, 1930, 2112
HapII	CCGG	2 cut(s)	271, 1281
HgaI	GACGC	1 cut(s)	1331
HhaI	GCGC	4 cut(s)	22, 597, 802, 1757
Hin6I	GCGC	4 cut(s)	20, 595, 800, 1755
HinP1I	GCGC	4 cut(s)	20, 595, 800, 1755
HindIII	AAGCTT	1 cut(s)	512
HinfI	GANTC	2 cut(s)	914, 1967
HpaII	CCGG	2 cut(s)	271, 1281
HphI	GGTGA	5 cut(s)	43, 212, 1163, 1295, 1667
Hpy166II	GTNNAC	3 cut(s)	1278, 1424, 1896
Hpy188I	TCNGA	7 cut(s)	631, 919, 979, 1480, 1700, 1727, 2117
Hpy8I	GTNNAC	3 cut(s)	1278, 1424, 1896
HpyAV	CCTTC	8 cut(s)	444, 1184, 1414, 1845, 1909, 1970, 2030, 2059
HpyCH4III	ACNGT	5 cut(s)	65, 171, 206, 718, 1650
HpyCH4IV	ACGT	1 cut(s)	344
HpyF10VI	GCNNNNNNNGC	3 cut(s)	762, 1397, 1446
HpyF3I	CTNAG	4 cut(s)	289, 847, 1477, 1697
HpySE526I	ACGT	1 cut(s)	344
HspAI	GCGC	4 cut(s)	20, 595, 800, 1755
KpnI	GGTACC	1 cut(s)	1134
Ksp22I	TGATCA	2 cut(s)	979, 1722
Kzo9I	GATC	9 cut(s)	490, 626, 974, 979, 1153, 1197, 1630, 1722, 1858
LmnI	GCTCC	1 cut(s)	1524
Lsp1109I	GCAGC	2 cut(s)	765, 838
LweI	GCATC	5 cut(s)	361, 1387, 1596, 1798, 1891
MaeI	CTAG	4 cut(s)	423, 2082, 2094, 2142
MaeII	ACGT	1 cut(s)	344
MaeIII	GTNAC	4 cut(s)	200, 211, 425, 1283
MalI	GATC	9 cut(s)	492, 628, 976, 981, 1155, 1199, 1632, 1724, 1860
MboI	GATC	9 cut(s)	490, 626, 974, 979, 1153, 1197, 1630, 1722, 1858
MfeI	CAATTG	1 cut(s)	1743
MflI	RGATCY	3 cut(s)	974, 1630, 1858
MhlI	GDGCHC	3 cut(s)	12, 702, 1066
MlyI	GAGTC	1 cut(s)	908
MmeI	TCCRAC	4 cut(s)	518, 847, 952, 1985
MroXI	GAANNNNTTC	1 cut(s)	1356
MseI	TTAA	6 cut(s)	231, 1020, 1143, 1577, 1730, 1949
MslI	CAYNNNNRTG	2 cut(s)	411, 556
MspA1I	CMGCKG	2 cut(s)	682, 851
MspI	CCGG	2 cut(s)	271, 1281
MspR9I	CCNGG	3 cut(s)	261, 1413, 2156
MunI	CAATTG	1 cut(s)	1743
Mva1269I	GAATGC	1 cut(s)	571
MvaI	CCWGG	3 cut(s)	261, 1413, 2156
MvnI	CGCG	1 cut(s)	1755
MwoI	GCNNNNNNNGC	3 cut(s)	762, 1397, 1446
NdeII	GATC	9 cut(s)	490, 626, 974, 979, 1153, 1197, 1630, 1722, 1858
NlaIV	GGNNCC	4 cut(s)	258, 534, 1064, 1132
NmuCI	GTSAC	2 cut(s)	200, 1283
NsbI	TGCGCA	2 cut(s)	21, 596
PagI	TCATGA	1 cut(s)	1074
PctI	GAATGC	1 cut(s)	571
PdmI	GAANNNNTTC	1 cut(s)	1356
PfeI	GAWTC	1 cut(s)	1967
PinAI	ACCGGT	2 cut(s)	270, 1280
PkrI	GCNGC	3 cut(s)	755, 853, 1754
PleI	GAGTC	1 cut(s)	908
PpsI	GAGTC	1 cut(s)	908
PshBI	ATTAAT	2 cut(s)	1577, 1730
Psp1406I	AACGTT	1 cut(s)	344
Psp6I	CCWGG	3 cut(s)	259, 1411, 2154
PspGI	CCWGG	3 cut(s)	259, 1411, 2154
PspN4I	GGNNCC	4 cut(s)	258, 534, 1064, 1132
PspOMI	GGGCCC	1 cut(s)	1062
PspPI	GGNCC	5 cut(s)	326, 335, 1062, 1063, 1928
PstI	CTGCAG	3 cut(s)	856, 946, 1563
PsuI	RGATCY	3 cut(s)	974, 1630, 1858
PvuII	CAGCTG	2 cut(s)	682, 851
RsaI	GTAC	9 cut(s)	161, 297, 486, 1132, 1208, 1298, 1573, 2022, 2045
RsaNI	GTAC	9 cut(s)	160, 296, 485, 1131, 1207, 1297, 1572, 2021, 2044
RseI	CAYNNNNRTG	2 cut(s)	411, 556
SaqAI	TTAA	6 cut(s)	231, 1020, 1143, 1577, 1730, 1949
SatI	GCNGC	3 cut(s)	754, 852, 1753
Sau3AI	GATC	9 cut(s)	490, 626, 974, 979, 1153, 1197, 1630, 1722, 1858
Sau96I	GGNCC	5 cut(s)	326, 335, 1062, 1063, 1928
ScaI	AGTACT	1 cut(s)	1298
SchI	GAGTC	1 cut(s)	908
ScrFI	CCNGG	3 cut(s)	261, 1413, 2156
SduI	GDGCHC	3 cut(s)	12, 702, 1066
SfaNI	GCATC	5 cut(s)	361, 1387, 1596, 1798, 1891
SfcI	CTRYAG	3 cut(s)	852, 942, 1559
SinI	GGWCC	1 cut(s)	335
SmiMI	CAYNNNNRTG	2 cut(s)	411, 556
SmlI	CTYRAG	2 cut(s)	1434, 1499
SmoI	CTYRAG	2 cut(s)	1434, 1499
SsiI	CCGC	5 cut(s)	286, 419, 784, 1440, 1753
SspI	AATATT	1 cut(s)	2078
SspMI	CTAG	4 cut(s)	423, 2082, 2094, 2142
StyD4I	CCNGG	3 cut(s)	259, 1411, 2154
TaaI	ACNGT	5 cut(s)	65, 171, 206, 718, 1650
TaiI	ACGT	1 cut(s)	347
TaqI	TCGA	3 cut(s)	932, 1181, 1902
TatI	WGTACW	5 cut(s)	159, 484, 1296, 1571, 2020
TauI	GCSGC	1 cut(s)	1755
TfiI	GAWTC	1 cut(s)	1967
Tru1I	TTAA	6 cut(s)	231, 1020, 1143, 1577, 1730, 1949
Tru9I	TTAA	6 cut(s)	231, 1020, 1143, 1577, 1730, 1949
TscAI	CASTG	7 cut(s)	29, 211, 533, 721, 916, 951, 1130
TseFI	GTSAC	2 cut(s)	200, 1283
TseI	GCWGC	2 cut(s)	753, 851
Tsp45I	GTSAC	2 cut(s)	200, 1283
TspGWI	ACGGA	4 cut(s)	471, 1217, 2045, 2061
TspRI	CASTG	7 cut(s)	29, 211, 533, 721, 916, 951, 1130
VpaK11BI	GGWCC	1 cut(s)	335
VspI	ATTAAT	2 cut(s)	1577, 1730
XapI	RAATTY	3 cut(s)	1183, 1781, 2165
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1518, 2105
XmnI	GAANNNNTTC	1 cut(s)	1356
XspI	CTAG	4 cut(s)	423, 2082, 2094, 2142
ZrmI	AGTACT	1 cut(s)	1298

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.