Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0003672

Physical Location & Seq

Genomic Coordinates Forward (+)

36694112 .. 36703394

Gene Structure 9283 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG274100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2700 bp

ATGGCTTCACTTTCACTTTCAACCAAGTTCCCTCTGTACCCCAACCTCACATTTCTCCAATTCCCTCTGTACCCAAACAGACCTCAAAGAATCATCTTTCCTATCAAGAAGAAAACCCAGATAGGCTGCTCCGCCACAGGTCAACCCCACCAACAACAAAGACCACCCAAGAACAAGAAGAAAATCCTCACCGACGGCGACAAAGGCATCGACCCAGTTGGCTTCCTCTCCAAATTCGACATCTCCCATAAGCAATTTGCTCAGTTCCTCCGTGAAAGACACAAAGCCCTCAAGGACCTGACAGATGAAATATTAACTCGCCACATCAACCTCAGGGATATGTCTTCTGGATATGAGATATTGGGTTTGCATCGACACCCAGAACATAGAGAGGATTATATGGAGTGGGCTCCAGGTGCTCGCTACTGCGCACTGGTTGGTGATTTCAATGGGTGGTCTCCCACAGAGAACTGTGCAAAAGAGGGTCATTTTGGACATGATGACTATGGATACTGGTTCATCATTCTTGAAGATAAGTTGAGGAATGGAGAGAAACCAGATGAGCTGTACTTTCAACAGTATAACTATGTAGATGATTACGATAAAGGTGACAGTGGTGTTACTATTGAAGAAGTCTTTAAGAAAGCAAATGATGAGTATTGGGAACCTGGAGAAGACCGGTTTCTAAAAAACCGCTTAGAAGTACCAGCAAAGTTGTATGAGCAGATTTTTGGGCCTAATGGACCTCAAACGTTAGAGGAACTTGAAGAAATACCAGATGCAGAAACAAGATATAAAGCGTGGAAAGAACAGCATAAAGATGACCCGCCTAGTAACTTACCTTCTTATGATGTGATTGAAAATGGAAAAGAGTATGACATTTACAATGTTGTACTTGATCCAGTATCACAAGAGAAATTTAAAGCAAAGAAGCCTCCCTTGCCATATTGGTTCGAAACACGTAAAGGAAGGAAGGCATGGTTGAAAAAGTATAGGCCGTGCATTCCTCATGGGAGCAAGTACAGGGTCTATTTTAATACTCCTAGTGGGCCACTGGAACGAGTACCTGCTTGGGCAACTTATGTGGAACCAGACGCAGATGGAGGACAAGCTTTTGCAATCCACTGGGAACCACCCCCAGAACTTGCATACAAATGGAAGAATGCACGCCCTAAAGTGCCAAAGTCCCTGCGCATTTATGAATGTCATGTTGGCATAAGTGGATCAGAGCCTAAAGTATCTTCTTTCAGTGATTTCACAGAGAAGGTTCTTCCTCATGTAAAGGAGGCTGGCTATAATGCAATTCAGCTGATTGGAGTTATTGAGCACAAAGATTATTTCACTGTTGGTTATAGAGCCACTAATTTATATGCTGTTAGCAGCCGATTTGGCACTCCTGATGATTTCAAGCGGTTGGTTGATGAAGCACATGGACAAGGACTGCTGGTCTTCTTGGAGATTGTCCATTCCTACTCGGCTGCAGATGAGATGGTTGGATTAGGACTCTTTGATGGAACAAATGACTGCTACTTCCATACTGGCAAACGGGGACATCACAAGCACTGGGGTACCAGAATGTTTAAATATGGTGATCTGGATGTTCTGCATTTCCTTCTCTCGAATTTAAACTGGTGGATCACGGAGTACCAAGTTGATGGTTTTCAGTTCCATTCACTCTCATCAATGATTTATACTCATAATGGTTTTGCTTCATTTACCGGTGACTTGGAGGAGTACTGCAATCAATATGTTGATAGGGACGCATTGTTATACCTCATTTTGGCAAACGAAATACTTCATGCTCTCCACCCAGATATAATAACGATTGCTGAAGATGCAACCTTCTATCCAGGTCTATGTGAACCAATCTCTCAAGGCGGATTGGGCTTTGATTATTGTGTCAATCTTTCTGTCTCAGAGATGTGGTCATCTTTCCTTGAGAATGTTCCAGACCATGATTGGAGCATGAGTAAGATAGTGAGTACATTAATGGGCAACAAAAATTATGCAGATAAGATGCTCATGTATGCTGAAAATCATAACCAGTCAATTTCTGGAGGGAGATCTTTTGCCGAGATACTGTTTGGTGAAATCAGGGAGGGCTCTCCTGACAAGGAGAAATTATTGCTCAGAGGTTGTTCCTTGCACAAGATGATCAGATTAAGTACCATGACAATTGGTGGCTGTGCTTATCTCAACTTCATGGGCAATGAATTTGGGCATCCAGAGAGGGTTGAGTTCCCAATGCCAAGCAACAATTTCTCATTTTCTCTTGCCAACCGAAGGTGGGATCTTCTTGCCAACGAGGGATTGCATCTCAACTTGTTTACCTTCGACAAGGACTTGATGAAGTTGGATGAAAATGAAAGAATACTTCCAAGAGTTTTACCCAGCATTCACCTTGTGAATGACAATAGCATGGTAATTGCTTACATAAGAGGGCCTCTTCTATTTGTATTTAACTTTCATCCAACAGATTCTTATGAAGGATACCGAATAGGTGTAGAAGAAGCTGGAGAGTATCAACTTGTACTAAATACGGATGAAAGGAAGTACGGAGGTCAAGGGCTTTTGAAGGATGACCAATATTCTAGGACAACTACTAGCCAAAGAAGTGATGGCCTCCGAAACAGCTTAGAAGTTCCTCTGCCCAGTAGGACTGCCCAGGTTTACAAATTAAGTCGGATTTTAAGAATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0007275 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009791 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0048856 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

899

Amino Acids

103.49

Weight (kDa)

5.9

Isoelectric Point (pI)

36.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Alpha-amylase	PF00128	424 - 492	1.1e-06	Alpha amylase, catalytic domain
Alpha-amylase_C	PF02806	802 - 893	6.8e-22	Alpha amylase, C-terminal all-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003672)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20440 AT3G20440 AT3G20440
fragaria_vesca	FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220
malus_domestica	MD11G1212700.v1.1
prunus_persica	Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1
pyrus_communis	pycom11g18770
rosa_chinensis	RchiOBHm_Chr5g0040901 RchiOBHm_Chr5g0040911
rosa_laevigata	RLG00000034012
rosa_multiflora	Rmu_sc0002848.1_g000016 Rmu_ssc0000416.1_g000003
rosa_roxburghii	Rroxscaffold_1G00039790
rosa_rugosa	Rorug05G0188500 Rorug05G0188600 Rorug05G0188600
rosa_samantha	Rh5AG274100 Rh5BG279100 Rh5CG311200 Rh5DG287300
rosa_wichuraiana	Rw5G025750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	430, 1193
Acc36I	ACCTGC	1 cut(s)	1075
Acc65I	GGTACC	1 cut(s)	1568
AccB1I	GGYRCC	1 cut(s)	1568
AciI	CCGC	5 cut(s)	132, 694, 827, 1411, 1878
AclI	AACGTT	1 cut(s)	752
AclWI	GGATC	4 cut(s)	893, 1231, 1643, 2298
AcsI	RAATTY	4 cut(s)	233, 917, 1621, 2213
AcuI	CTGAAG	1 cut(s)	1851
AdeI	CACNNNGTG	1 cut(s)	2404
AfiI	CCNNNNNNNGG	3 cut(s)	1780, 2283, 2287
AflIII	ACRYGT	1 cut(s)	959
AgeI	ACCGGT	2 cut(s)	678, 1718
AhdI	GACNNNNNGTC	1 cut(s)	1445
AjnI	CCWGG	4 cut(s)	412, 667, 1849, 2664
AjuI	GAANNNNNNNTTGG	2 cut(s)	1194, 1226
AluBI	AGCT	5 cut(s)	565, 1112, 1309, 2513, 2634
AluI	AGCT	5 cut(s)	565, 1112, 1309, 2513, 2634
Alw21I	GWGCWC	2 cut(s)	421, 1329
Alw26I	GTCTC	2 cut(s)	462, 1918
AlwI	GGATC	4 cut(s)	893, 1231, 1643, 2298
AoxI	GGCC	5 cut(s)	734, 995, 1049, 2441, 2620
ApeKI	GCWGC	3 cut(s)	126, 1380, 1478
ApoI	RAATTY	4 cut(s)	233, 917, 1621, 2213
AseI	ATTAAT	1 cut(s)	1988
AsiGI	ACCGGT	2 cut(s)	678, 1718
Asp700I	GAANNNNTTC	1 cut(s)	1794
Asp718I	GGTACC	1 cut(s)	1568
AspLEI	GCGC	2 cut(s)	431, 1194
AspS9I	GGNCC	5 cut(s)	295, 734, 743, 1049, 2441
AsuHPI	GGTGA	7 cut(s)	181, 452, 620, 1601, 1733, 2099, 2390
AsuII	TTCGAA	1 cut(s)	954
AvaII	GGWCC	2 cut(s)	295, 743
AxyI	CCTNAGG	1 cut(s)	332
BaeI	ACNNNNGTAYC	2 cut(s)	1552, 1585
BanI	GGYRCC	1 cut(s)	1568
BanII	GRGCYC	2 cut(s)	412, 2105
BarI	GAAGNNNNNNTAC	2 cut(s)	826, 858
BbsI	GAAGAC	3 cut(s)	336, 681, 1441
Bbv12I	GWGCWC	2 cut(s)	421, 1329
BbvI	GCAGC	3 cut(s)	113, 1392, 1465
BccI	CCATC	5 cut(s)	1094, 1483, 1505, 1649, 2612
BceAI	ACGGC	2 cut(s)	211, 982
BciT130I	CCWGG	4 cut(s)	414, 669, 1851, 2666
BciVI	GTATCC	2 cut(s)	503, 2483
BclI	TGATCA	1 cut(s)	2154
BcoDI	GTCTC	2 cut(s)	462, 1918
BfaI	CTAG	4 cut(s)	831, 1044, 2592, 2604
BfmI	CTRYAG	1 cut(s)	1479
BfuAI	ACCTGC	1 cut(s)	1075
BfuI	GTATCC	2 cut(s)	503, 2483
BglI	GCCNNNNNGGC	1 cut(s)	1389
BglII	AGATCT	1 cut(s)	2063
BisI	GCNGC	3 cut(s)	127, 1381, 1479
BlsI	GCNGC	3 cut(s)	128, 1382, 1480
BmcAI	AGTACT	1 cut(s)	1736
Bme1390I	CCNGG	4 cut(s)	414, 669, 1851, 2666
Bme18I	GGWCC	2 cut(s)	295, 743
BmeRI	GACNNNNNGTC	1 cut(s)	1445
BmgT120I	GGNCC	5 cut(s)	295, 734, 743, 1049, 2441
BmiI	GGNNCC	5 cut(s)	411, 666, 1089, 1131, 1570
BmrFI	CCNGG	4 cut(s)	414, 669, 1851, 2666
BmrI	ACTGGG	4 cut(s)	209, 1135, 1573, 2646
BmsI	GCATC	7 cut(s)	216, 379, 769, 1825, 2007, 2230, 2323
BmuI	ACTGGG	4 cut(s)	209, 1135, 1573, 2646
BpiI	GAAGAC	3 cut(s)	336, 681, 1441
BpmI	CTGGAG	4 cut(s)	396, 690, 2076, 2535
Bpu14I	TTCGAA	1 cut(s)	954
BpuEI	CTTGAG	3 cut(s)	275, 1857, 1958
BsaAI	YACGTR	1 cut(s)	962
BsaI	GGTCTC	1 cut(s)	462
BsaJI	CCNNGG	1 cut(s)	2664
BsaWI	WCCGGW	2 cut(s)	678, 1718
BsaXI	ACNNNNNCTCC	2 cut(s)	1954, 1984
Bsc4I	CCNNNNNNNGG	3 cut(s)	1780, 2283, 2287
Bse118I	RCCGGY	2 cut(s)	678, 1718
Bse21I	CCTNAGG	1 cut(s)	332
Bse3DI	GCAATG	1 cut(s)	2215
BseBI	CCWGG	4 cut(s)	414, 669, 1851, 2666
BseDI	CCNNGG	1 cut(s)	2664
BseGI	GGATG	6 cut(s)	1603, 2221, 2362, 2467, 2548, 2584
BseLI	CCNNNNNNNGG	3 cut(s)	1780, 2283, 2287
BseMI	GCAATG	1 cut(s)	2215
BseMII	CTCAG	4 cut(s)	275, 346, 1929, 2143
BseRI	GAGGAG	1 cut(s)	1745
BseXI	GCAGC	3 cut(s)	113, 1392, 1465
BseYI	CCCAGC	1 cut(s)	2390
BshFI	GGCC	5 cut(s)	736, 997, 1051, 2443, 2622
BshNI	GGYRCC	1 cut(s)	1568
BshTI	ACCGGT	2 cut(s)	678, 1718
BsiHKAI	GWGCWC	2 cut(s)	421, 1329
BsiSI	CCGG	2 cut(s)	679, 1719
BslFI	GGGAC	3 cut(s)	1171, 1563, 1772
BslI	CCNNNNNNNGG	3 cut(s)	1780, 2283, 2287
BsmAI	GTCTC	2 cut(s)	462, 1918
BsmFI	GGGAC	3 cut(s)	1171, 1563, 1772
BsmI	GAATGC	3 cut(s)	1002, 1168, 2394
BsnI	GGCC	5 cut(s)	736, 997, 1051, 2443, 2622
Bso31I	GGTCTC	1 cut(s)	462
Bsp119I	TTCGAA	1 cut(s)	954
Bsp1286I	GDGCHC	4 cut(s)	412, 421, 1329, 2105
Bsp143I	GATC	7 cut(s)	898, 1223, 1591, 1635, 2063, 2154, 2290
BspACI	CCGC	5 cut(s)	132, 694, 827, 1411, 1878
BspANI	GGCC	5 cut(s)	736, 997, 1051, 2443, 2622
BspCNI	CTCAG	4 cut(s)	274, 345, 1928, 2142
BspLI	GGNNCC	5 cut(s)	411, 666, 1089, 1131, 1570
BspMAI	CTGCAG	1 cut(s)	1483
BspMI	ACCTGC	1 cut(s)	1075
BspPI	GGATC	4 cut(s)	893, 1231, 1643, 2298
BspT104I	TTCGAA	1 cut(s)	954
BspT107I	GGYRCC	1 cut(s)	1568
BspTNI	GGTCTC	1 cut(s)	462
BsrDI	GCAATG	1 cut(s)	2215
BsrFI	RCCGGY	2 cut(s)	678, 1718
BssAI	RCCGGY	2 cut(s)	678, 1718
BssECI	CCNNGG	1 cut(s)	2664
BssMI	GATC	7 cut(s)	898, 1223, 1591, 1635, 2063, 2154, 2290
Bst2UI	CCWGG	4 cut(s)	414, 669, 1851, 2666
Bst4CI	ACNGT	5 cut(s)	473, 579, 614, 1345, 2082
Bst6I	CTCTTC	1 cut(s)	2451
BstBAI	YACGTR	1 cut(s)	962
BstBI	TTCGAA	1 cut(s)	954
BstC8I	GCNNGC	3 cut(s)	421, 1168, 1291
BstDEI	CTNAG	6 cut(s)	261, 332, 697, 1915, 2129, 2635
BstF5I	GGATG	6 cut(s)	1603, 2221, 2362, 2467, 2548, 2584
BstHHI	GCGC	2 cut(s)	431, 1194
BstKTI	GATC	7 cut(s)	901, 1226, 1594, 1638, 2066, 2157, 2293
BstMAI	GTCTC	2 cut(s)	462, 1918
BstMBI	GATC	7 cut(s)	898, 1223, 1591, 1635, 2063, 2154, 2290
BstMWI	GCNNNNNNNGC	6 cut(s)	204, 416, 940, 1389, 1835, 1884
BstNI	CCWGG	4 cut(s)	414, 669, 1851, 2666
BstSCI	CCNGG	4 cut(s)	412, 667, 1849, 2664
BstSFI	CTRYAG	1 cut(s)	1479
BstV1I	GCAGC	3 cut(s)	113, 1392, 1465
BstV2I	GAAGAC	3 cut(s)	336, 681, 1441
BstX2I	RGATCY	2 cut(s)	2063, 2290
BstXI	CCANNNNNNTGG	1 cut(s)	1655
BstYI	RGATCY	2 cut(s)	2063, 2290
Bsu36I	CCTNAGG	1 cut(s)	332
BsuI	GTATCC	2 cut(s)	503, 2483
BsuRI	GGCC	5 cut(s)	736, 997, 1051, 2443, 2622
BtsCI	GGATG	6 cut(s)	1603, 2221, 2362, 2467, 2548, 2584
BtsIMutI	CAGTG	7 cut(s)	431, 619, 1052, 1123, 1255, 1341, 1561
BveI	ACCTGC	1 cut(s)	1075
Cac8I	GCNNGC	3 cut(s)	421, 1168, 1291
CfoI	GCGC	2 cut(s)	431, 1194
Cfr10I	RCCGGY	2 cut(s)	678, 1718
Cfr13I	GGNCC	5 cut(s)	295, 734, 743, 1049, 2441
CseI	GACGC	2 cut(s)	1103, 1769
CspAI	ACCGGT	2 cut(s)	678, 1718
CspCI	CAANNNNNGTGG	2 cut(s)	1065, 1100
DdeI	CTNAG	6 cut(s)	261, 332, 697, 1915, 2129, 2635
DpnI	GATC	7 cut(s)	900, 1225, 1593, 1637, 2065, 2156, 2292
DpnII	GATC	7 cut(s)	898, 1223, 1591, 1635, 2063, 2154, 2290
DraI	TTTAAA	3 cut(s)	922, 1582, 1626
DraIII	CACNNNGTG	1 cut(s)	2404
DriI	GACNNNNNGTC	1 cut(s)	1445
Eam1104I	CTCTTC	1 cut(s)	2451
Eam1105I	GACNNNNNGTC	1 cut(s)	1445
EarI	CTCTTC	1 cut(s)	2451
EciI	GGCGGA	2 cut(s)	121, 1893
Eco24I	GRGCYC	2 cut(s)	412, 2105
Eco31I	GGTCTC	1 cut(s)	462
Eco47I	GGWCC	2 cut(s)	295, 743
Eco57I	CTGAAG	1 cut(s)	1851
Eco81I	CCTNAGG	1 cut(s)	332
EcoO109I	RGGNCCY	2 cut(s)	295, 2441
EcoRII	CCWGG	4 cut(s)	412, 667, 1849, 2664
EcoT38I	GRGCYC	2 cut(s)	412, 2105
FalI	AAGNNNNNCTT	2 cut(s)	923, 955
FaqI	GGGAC	3 cut(s)	1171, 1563, 1772
FauI	CCCGC	1 cut(s)	834
FbaI	TGATCA	1 cut(s)	2154
Fnu4HI	GCNGC	3 cut(s)	127, 1381, 1479
FokI	GGATG	6 cut(s)	1610, 2208, 2369, 2454, 2555, 2591
FriOI	GRGCYC	2 cut(s)	412, 2105
Fsp4HI	GCNGC	3 cut(s)	127, 1381, 1479
FspBI	CTAG	4 cut(s)	831, 1044, 2592, 2604
FspI	TGCGCA	2 cut(s)	430, 1193
GlaI	GCGC	2 cut(s)	430, 1193
GluI	GCNGC	3 cut(s)	127, 1381, 1479
GsaI	CCCAGC	1 cut(s)	2394
GsuI	CTGGAG	4 cut(s)	396, 690, 2076, 2535
HaeIII	GGCC	5 cut(s)	736, 997, 1051, 2443, 2622
HapII	CCGG	2 cut(s)	679, 1719
HgaI	GACGC	2 cut(s)	1103, 1769
HhaI	GCGC	2 cut(s)	431, 1194
Hin6I	GCGC	2 cut(s)	429, 1192
HinP1I	GCGC	2 cut(s)	429, 1192
HincII	GTYRAC	1 cut(s)	143
HindII	GTYRAC	1 cut(s)	143
HindIII	AAGCTT	1 cut(s)	1110
HinfI	GANTC	3 cut(s)	90, 1503, 2477
HpaII	CCGG	2 cut(s)	679, 1719
HphI	GGTGA	7 cut(s)	181, 452, 620, 1601, 1733, 2099, 2390
Hpy166II	GTNNAC	4 cut(s)	143, 1862, 2328, 2671
Hpy188I	TCNGA	6 cut(s)	1228, 1918, 2132, 2159, 2627, 2685
Hpy8I	GTNNAC	4 cut(s)	143, 1862, 2328, 2671
Hpy99I	CGWCG	1 cut(s)	197
HpyCH4III	ACNGT	5 cut(s)	473, 579, 614, 1345, 2082
HpyCH4IV	ACGT	2 cut(s)	752, 961
HpyF10VI	GCNNNNNNNGC	6 cut(s)	204, 416, 940, 1389, 1835, 1884
HpyF3I	CTNAG	6 cut(s)	261, 332, 697, 1915, 2129, 2635
HpySE526I	ACGT	2 cut(s)	752, 961
HspAI	GCGC	2 cut(s)	429, 1192
KpnI	GGTACC	1 cut(s)	1572
Ksp22I	TGATCA	1 cut(s)	2154
Kzo9I	GATC	7 cut(s)	898, 1223, 1591, 1635, 2063, 2154, 2290
LmnI	GCTCC	4 cut(s)	134, 415, 1014, 1962
Lsp1109I	GCAGC	3 cut(s)	113, 1392, 1465
LweI	GCATC	7 cut(s)	216, 379, 769, 1825, 2007, 2230, 2323
MaeI	CTAG	4 cut(s)	831, 1044, 2592, 2604
MaeII	ACGT	2 cut(s)	752, 961
MaeIII	GTNAC	4 cut(s)	608, 619, 833, 1721
MalI	GATC	7 cut(s)	900, 1225, 1593, 1637, 2065, 2156, 2292
MboI	GATC	7 cut(s)	898, 1223, 1591, 1635, 2063, 2154, 2290
MfeI	CAATTG	1 cut(s)	2175
MflI	RGATCY	2 cut(s)	2063, 2290
MhlI	GDGCHC	4 cut(s)	412, 421, 1329, 2105
MlyI	GAGTC	1 cut(s)	1497
MmeI	TCCRAC	4 cut(s)	1474, 2334, 2495, 2663
MroXI	GAANNNNTTC	1 cut(s)	1794
MslI	CAYNNNNRTG	2 cut(s)	819, 1153
MspA1I	CMGCKG	1 cut(s)	1309
MspI	CCGG	2 cut(s)	679, 1719
MspR9I	CCNGG	4 cut(s)	414, 669, 1851, 2666
MunI	CAATTG	1 cut(s)	2175
Mva1269I	GAATGC	3 cut(s)	1002, 1168, 2394
MvaI	CCWGG	4 cut(s)	414, 669, 1851, 2666
MwoI	GCNNNNNNNGC	6 cut(s)	204, 416, 940, 1389, 1835, 1884
NdeII	GATC	7 cut(s)	898, 1223, 1591, 1635, 2063, 2154, 2290
NlaIV	GGNNCC	5 cut(s)	411, 666, 1089, 1131, 1570
NmeAIII	GCCGAG	2 cut(s)	1454, 2098
NmuCI	GTSAC	2 cut(s)	608, 1721
NsbI	TGCGCA	2 cut(s)	430, 1193
NspV	TTCGAA	1 cut(s)	954
PctI	GAATGC	3 cut(s)	1002, 1168, 2394
PdmI	GAANNNNTTC	1 cut(s)	1794
PfeI	GAWTC	2 cut(s)	90, 2477
PinAI	ACCGGT	2 cut(s)	678, 1718
PkrI	GCNGC	3 cut(s)	128, 1382, 1480
PleI	GAGTC	1 cut(s)	1497
PpsI	GAGTC	1 cut(s)	1497
Ppu21I	YACGTR	1 cut(s)	962
PpuMI	RGGWCCY	1 cut(s)	295
PshBI	ATTAAT	1 cut(s)	1988
Psp1406I	AACGTT	1 cut(s)	752
Psp5II	RGGWCCY	1 cut(s)	295
Psp6I	CCWGG	4 cut(s)	412, 667, 1849, 2664
PspFI	CCCAGC	1 cut(s)	2390
PspGI	CCWGG	4 cut(s)	412, 667, 1849, 2664
PspN4I	GGNNCC	5 cut(s)	411, 666, 1089, 1131, 1570
PspPI	GGNCC	5 cut(s)	295, 734, 743, 1049, 2441
PspPPI	RGGWCCY	1 cut(s)	295
PstI	CTGCAG	1 cut(s)	1483
PsuI	RGATCY	2 cut(s)	2063, 2290
PvuII	CAGCTG	1 cut(s)	1309
RseI	CAYNNNNRTG	2 cut(s)	819, 1153
SatI	GCNGC	3 cut(s)	127, 1381, 1479
Sau3AI	GATC	7 cut(s)	898, 1223, 1591, 1635, 2063, 2154, 2290
Sau96I	GGNCC	5 cut(s)	295, 734, 743, 1049, 2441
ScaI	AGTACT	1 cut(s)	1736
SchI	GAGTC	1 cut(s)	1497
ScrFI	CCNGG	4 cut(s)	414, 669, 1851, 2666
SduI	GDGCHC	4 cut(s)	412, 421, 1329, 2105
SfaNI	GCATC	7 cut(s)	216, 379, 769, 1825, 2007, 2230, 2323
SfcI	CTRYAG	1 cut(s)	1479
SfuI	TTCGAA	1 cut(s)	954
SinI	GGWCC	2 cut(s)	295, 743
SmiMI	CAYNNNNRTG	2 cut(s)	819, 1153
SmlI	CTYRAG	3 cut(s)	290, 1872, 1937
SmoI	CTYRAG	3 cut(s)	290, 1872, 1937
SsiI	CCGC	5 cut(s)	132, 694, 827, 1411, 1878
SspI	AATATT	2 cut(s)	312, 2588
SspMI	CTAG	4 cut(s)	831, 1044, 2592, 2604
StyD4I	CCNGG	4 cut(s)	412, 667, 1849, 2664
TaaI	ACNGT	5 cut(s)	473, 579, 614, 1345, 2082
TaiI	ACGT	2 cut(s)	755, 964
TaqI	TCGA	6 cut(s)	210, 237, 373, 954, 1619, 2334
TatI	WGTACW	6 cut(s)	567, 892, 1020, 1734, 1982, 2530
TfiI	GAWTC	2 cut(s)	90, 2477
TscAI	CASTG	7 cut(s)	438, 619, 1059, 1130, 1255, 1348, 1568
TseFI	GTSAC	2 cut(s)	608, 1721
TseI	GCWGC	3 cut(s)	126, 1380, 1478
Tsp45I	GTSAC	2 cut(s)	608, 1721
TspGWI	ACGGA	4 cut(s)	260, 1655, 2555, 2571
TspRI	CASTG	7 cut(s)	438, 619, 1059, 1130, 1255, 1348, 1568
VpaK11BI	GGWCC	2 cut(s)	295, 743
VspI	ATTAAT	1 cut(s)	1988
XapI	RAATTY	4 cut(s)	233, 917, 1621, 2213
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1956, 2051, 2615
XmnI	GAANNNNTTC	1 cut(s)	1794
XspI	CTAG	4 cut(s)	831, 1044, 2592, 2604
ZrmI	AGTACT	1 cut(s)	1736

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.