Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0003672

Physical Location & Seq

Genomic Coordinates Forward (+)

35313693 .. 35323025

Gene Structure 9333 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000034012

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2694 bp

ATGGCTTCACTTTCTCTTTCAACTAAGTTCACTCTGTACCCCAACCTCACATTTCTCCAATTCCAGTACCCAAACAGACCTCAAAGAATCATCTTTCCTATCAAGAAGAAAACCCAGATAGGCTGCTCCGCCACAGGTCAACCCCACCAACAACAAAGACCCCCCAAGAAGAAGACAACCCTCACCGACGGCGACAAAGGCGTCGACCCAGTTGGTTTCCTCTCCAAATTCAACATCTCCCATAAGCAGTTTGCTCAGTTCCTCCGTGAAAGACATAAAGCTTTGAAGGACCTGACAGATGAAATATTAAATCGGCACATTAACCTCAGGGATATGTCTTCTGGATATGAGATATTGGGTTTGCATCGACACCCAGAACATAGAGAGGATTATATGGAGTGGGCTCCAGGTGCTCGCTACTGCGCACTGGTTGGTGATTTCAATGGGTGGTCTCCCACAGAGAACTGTGCAAAAGAGGGTCATTTTGGACATGATGACTATGGATACTGGTTCATCATTCTTGAAGATAAGTTGAGGAATGGAGAGAAACCAGATGAGCTGTACTTTCAACAGTATAACTATGTAGATGATTACGATAAAGGTGACAGTGGTGTTACCATTGAAGAAGTCTTTAAGAAAGCAAATGATGAGTATTGGGAACCTGGAGAAGACCGGTTTCTAAAAAACCGCTTAGAAGTACCAGCAAAGTTGTATGAGCAGATTTTTGGGCCTAATGGACCTCAAACATTAGAGGAACTTGAAGAAATACCAGATGCAGAAACAAGATATAAAGCGTGGAAAGAACAGCATAAAGATGACCCGCCTAGTAACTTACCTTCTTATGATGTGATTGACAATGGAAAAGAGTATGACATTTACAATGTTGTACTTGATCCAGTATCACAAGAGAAATTTAAAGCAAAGAAGCCTCCCTTACCATATTGGTTCGAGACACGTAAAGGAAGGAAGGCATGGTTGAAAAAGTATAGGCCGTGCATTCCTCATGGGAGCAAGTACAGGGTCTATTTTAATACTCCTAGTGGGCCACTGGAACGAGTACCTGCTTGGGCAACTTATGTGGAACCAGACGCAGATGGAGGACAAGCTTTTGCAATCCACTGGGAACCACCCCCAGAACTTGCATACAAATGGAAGAATGCACGCCCTAAAGTGCCAAAGTCCCTGCGCATTTATGAATGTCATGTTGGCATAAGTGGATCAGAGCCTAAAGTATCTTCTTTCAGTGATTTCACAGAGAAGGTTCTTCCTCATGTAAAGGAGGCTGGCTATAATGCAATTCAGCTGATTGGAGTTATTGAGCACAAAGATTATTTCACTGTTGGTTATAGAGCCACTAATTTATATGCTGTTAGCAGCCGATTTGGCACTCCTGATGATTTCAAGCGGTTGGTTGATGAAGCACATGGACAAGGACTGCTGGTCTTCTTGGAGATTGTCCATTCCTACTCAGCTGCAGATGAGATGGTTGGATTAGGGCTTTTTGATGGAACAAATGACTGCTACTTCCACACTGGCAAGCGGGGACATCACAAGCACTGGGGTACCAGAATGTTTAAATATGGTGATCTGGATGTTCTGCATTTCCTTCTCTCGAATTTGAACTGGTGGATCACGGAGTACCAAGTTGATGGTTTTCAGTTCCATTCACTCTCATCAATGATTTATACTCATAATGGTTTTGCTTCGTTTACCGGTGACTTGGAGGAGTACTGCAATCAATATGTTGATAGGGACGCATTGTTATACCTCATTTTGGCAAACGAAATACTTCATGCTCTCCACCCAGATATAATAATGATTGCTGAAGATGCAACCTTCTATCCAGGTCTATGTGAACCAATCTCTCAAGGCGGATTGGGCTTTGATTATTGTGTCAATCTTTCTGTCTCAGAGATGTGGTCATCTTTCCTTGAGAATGTTCCAGACCATGATTGGAGCATGAGTAAGATTGTGAGTACATTAATGGGCAACAAAAATTATGCAGATAAGATGCTCATGTATGCTGAAAATCATAACCAGTCAATTTCTGGAGGGAGATCTTTTGCAGAGATACTGTTTGGTGAAATCAGGGAAGGCTCTCCTGACAAGGAGAAATTATTGCTCAGAGGTTGTTCCTTGCACAAGATGATCAGATTAATTACCATGACAATTGGTGGCCGTGCTTATCTCAACTTCATGGGCAATGAATTTGGGCATCCAGAGAGGGTTGAGTTCCCAATGCCAAGCAACAATTTCTCATTTTCTCTTGCCAACCGAAGGTGGGATCTTCTTGCCAATGAGGGATTGCATCTCAACTTGTTTACCTTCGATAAGGACTTGATGAAGTTGGATGAAAATGAAAGAATACTTCCAAGAGTTTTACCCAGCATTCACCATGTGAATGACAATAGCATGGTAATTGCTTACATAAGAGGGCCTCTTCTATTCGTATTTAACTTTCATCCAACAGATTCTTATGAAGGATACCGAATAGGTATAGAAGAAGCTGGAGAGTATCAACTTGTACTAAATACGGATCAAAGGAAGTACGGAGGTCAGGGGCTTTTGAAGGATGACCAATATTCTAGGACAACTACTAGCCAAAGAAGTGATGGCCTCCGAAACAGCTTAGAAGTTCCTCTGCCTAGTAGGACTGCCCAGGTTTACAAATTAAGTCGGATTTTAAGAATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0007275 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009791 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0048856 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

898

Amino Acids

103.39

Weight (kDa)

5.99

Isoelectric Point (pI)

35.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Alpha-amylase	PF00128	422 - 490	1.1e-06	Alpha amylase, catalytic domain
Alpha-amylase_C	PF02806	799 - 891	1.3e-21	Alpha amylase, C-terminal all-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003672)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20440 AT3G20440 AT3G20440
fragaria_vesca	FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220
malus_domestica	MD11G1212700.v1.1
prunus_persica	Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1
pyrus_communis	pycom11g18770
rosa_chinensis	RchiOBHm_Chr5g0040901 RchiOBHm_Chr5g0040911
rosa_laevigata	RLG00000034012
rosa_multiflora	Rmu_sc0002848.1_g000016 Rmu_ssc0000416.1_g000003
rosa_roxburghii	Rroxscaffold_1G00039790
rosa_rugosa	Rorug05G0188500 Rorug05G0188600 Rorug05G0188600
rosa_samantha	Rh5AG274100 Rh5BG279100 Rh5CG311200 Rh5DG287300
rosa_wichuraiana	Rw5G025750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	200
Acc16I	TGCGCA	2 cut(s)	424, 1187
Acc36I	ACCTGC	1 cut(s)	1069
Acc65I	GGTACC	1 cut(s)	1562
AccB1I	GGYRCC	1 cut(s)	1562
AccI	GTMKAC	1 cut(s)	204
AciI	CCGC	6 cut(s)	129, 688, 821, 1405, 1540, 1872
AclWI	GGATC	5 cut(s)	887, 1225, 1637, 2292, 2544
AcoI	YGGCCR	1 cut(s)	2176
AcsI	RAATTY	4 cut(s)	227, 911, 1615, 2207
AcuI	CTGAAG	1 cut(s)	1845
AcyI	GRCGYC	1 cut(s)	201
AdeI	CACNNNGTG	1 cut(s)	2398
AfiI	CCNNNNNNNGG	3 cut(s)	1774, 2277, 2281
AflIII	ACRYGT	1 cut(s)	953
AgeI	ACCGGT	2 cut(s)	672, 1712
AhdI	GACNNNNNGTC	1 cut(s)	1439
AjnI	CCWGG	4 cut(s)	406, 661, 1843, 2658
AjuI	GAANNNNNNNTTGG	2 cut(s)	1188, 1220
AluBI	AGCT	7 cut(s)	281, 559, 1106, 1303, 1472, 2507, 2628
AluI	AGCT	7 cut(s)	281, 559, 1106, 1303, 1472, 2507, 2628
Alw21I	GWGCWC	2 cut(s)	415, 1323
Alw26I	GTCTC	3 cut(s)	456, 944, 1912
AlwI	GGATC	5 cut(s)	887, 1225, 1637, 2292, 2544
AoxI	GGCC	6 cut(s)	728, 989, 1043, 2176, 2435, 2614
ApeKI	GCWGC	3 cut(s)	123, 1374, 1472
ApoI	RAATTY	4 cut(s)	227, 911, 1615, 2207
AseI	ATTAAT	2 cut(s)	1982, 2156
AsiGI	ACCGGT	2 cut(s)	672, 1712
Asp700I	GAANNNNTTC	1 cut(s)	1788
Asp718I	GGTACC	1 cut(s)	1562
AspLEI	GCGC	2 cut(s)	425, 1188
AspS9I	GGNCC	5 cut(s)	289, 728, 737, 1043, 2435
AsuHPI	GGTGA	7 cut(s)	175, 446, 614, 1595, 1727, 2093, 2384
AvaII	GGWCC	2 cut(s)	289, 737
AxyI	CCTNAGG	1 cut(s)	326
BaeI	ACNNNNGTAYC	2 cut(s)	1546, 1579
BanI	GGYRCC	1 cut(s)	1562
BanII	GRGCYC	1 cut(s)	406
BarI	GAAGNNNNNNTAC	2 cut(s)	820, 852
BbsI	GAAGAC	4 cut(s)	179, 330, 675, 1435
Bbv12I	GWGCWC	2 cut(s)	415, 1323
BbvI	GCAGC	3 cut(s)	110, 1386, 1459
BccI	CCATC	5 cut(s)	1088, 1477, 1499, 1643, 2606
BceAI	ACGGC	3 cut(s)	205, 976, 2163
BciT130I	CCWGG	4 cut(s)	408, 663, 1845, 2660
BciVI	GTATCC	2 cut(s)	497, 2477
BclI	TGATCA	1 cut(s)	2148
BcoDI	GTCTC	3 cut(s)	456, 944, 1912
BfaI	CTAG	5 cut(s)	825, 1038, 2586, 2598, 2646
BfmI	CTRYAG	1 cut(s)	1473
BfuAI	ACCTGC	1 cut(s)	1069
BfuI	GTATCC	2 cut(s)	497, 2477
BglI	GCCNNNNNGGC	1 cut(s)	1383
BglII	AGATCT	1 cut(s)	2057
BisI	GCNGC	3 cut(s)	124, 1375, 1473
BlsI	GCNGC	3 cut(s)	125, 1376, 1474
BmcAI	AGTACT	1 cut(s)	1730
Bme1390I	CCNGG	4 cut(s)	408, 663, 1845, 2660
Bme18I	GGWCC	2 cut(s)	289, 737
BmeRI	GACNNNNNGTC	1 cut(s)	1439
BmgT120I	GGNCC	5 cut(s)	289, 728, 737, 1043, 2435
BmiI	GGNNCC	5 cut(s)	405, 660, 1083, 1125, 1564
BmrFI	CCNGG	4 cut(s)	408, 663, 1845, 2660
BmrI	ACTGGG	3 cut(s)	203, 1129, 1567
BmsI	GCATC	6 cut(s)	373, 763, 1819, 2001, 2224, 2317
BmuI	ACTGGG	3 cut(s)	203, 1129, 1567
BpiI	GAAGAC	4 cut(s)	179, 330, 675, 1435
BpmI	CTGGAG	4 cut(s)	390, 684, 2070, 2529
BpuEI	CTTGAG	2 cut(s)	1851, 1952
BsaAI	YACGTR	1 cut(s)	956
BsaHI	GRCGYC	1 cut(s)	201
BsaI	GGTCTC	1 cut(s)	456
BsaJI	CCNNGG	1 cut(s)	2658
BsaWI	WCCGGW	2 cut(s)	672, 1712
BsaXI	ACNNNNNCTCC	2 cut(s)	1948, 1978
Bsc4I	CCNNNNNNNGG	3 cut(s)	1774, 2277, 2281
Bse118I	RCCGGY	2 cut(s)	672, 1712
Bse21I	CCTNAGG	1 cut(s)	326
Bse3DI	GCAATG	1 cut(s)	2209
BseBI	CCWGG	4 cut(s)	408, 663, 1845, 2660
BseDI	CCNNGG	1 cut(s)	2658
BseGI	GGATG	5 cut(s)	1597, 2215, 2356, 2461, 2578
BseLI	CCNNNNNNNGG	3 cut(s)	1774, 2277, 2281
BseMI	GCAATG	1 cut(s)	2209
BseMII	CTCAG	5 cut(s)	269, 340, 1482, 1923, 2137
BseRI	GAGGAG	1 cut(s)	1739
BseXI	GCAGC	3 cut(s)	110, 1386, 1459
BseYI	CCCAGC	1 cut(s)	2384
BshFI	GGCC	6 cut(s)	730, 991, 1045, 2178, 2437, 2616
BshNI	GGYRCC	1 cut(s)	1562
BshTI	ACCGGT	2 cut(s)	672, 1712
BsiHKAI	GWGCWC	2 cut(s)	415, 1323
BsiSI	CCGG	2 cut(s)	673, 1713
BslFI	GGGAC	3 cut(s)	1165, 1557, 1766
BslI	CCNNNNNNNGG	3 cut(s)	1774, 2277, 2281
BsmAI	GTCTC	3 cut(s)	456, 944, 1912
BsmFI	GGGAC	3 cut(s)	1165, 1557, 1766
BsmI	GAATGC	3 cut(s)	996, 1162, 2388
BsnI	GGCC	6 cut(s)	730, 991, 1045, 2178, 2437, 2616
Bso31I	GGTCTC	1 cut(s)	456
Bsp1286I	GDGCHC	3 cut(s)	406, 415, 1323
Bsp143I	GATC	8 cut(s)	892, 1217, 1585, 1629, 2057, 2148, 2284, 2536
BspACI	CCGC	6 cut(s)	129, 688, 821, 1405, 1540, 1872
BspANI	GGCC	6 cut(s)	730, 991, 1045, 2178, 2437, 2616
BspCNI	CTCAG	5 cut(s)	268, 339, 1481, 1922, 2136
BspLI	GGNNCC	5 cut(s)	405, 660, 1083, 1125, 1564
BspMAI	CTGCAG	1 cut(s)	1477
BspMI	ACCTGC	1 cut(s)	1069
BspPI	GGATC	5 cut(s)	887, 1225, 1637, 2292, 2544
BspT107I	GGYRCC	1 cut(s)	1562
BspTNI	GGTCTC	1 cut(s)	456
BsrDI	GCAATG	1 cut(s)	2209
BsrFI	RCCGGY	2 cut(s)	672, 1712
BssAI	RCCGGY	2 cut(s)	672, 1712
BssECI	CCNNGG	1 cut(s)	2658
BssMI	GATC	8 cut(s)	892, 1217, 1585, 1629, 2057, 2148, 2284, 2536
BssNI	GRCGYC	1 cut(s)	201
Bst2UI	CCWGG	4 cut(s)	408, 663, 1845, 2660
Bst4CI	ACNGT	5 cut(s)	467, 573, 608, 1339, 2076
Bst6I	CTCTTC	1 cut(s)	2445
BstACI	GRCGYC	1 cut(s)	201
BstBAI	YACGTR	1 cut(s)	956
BstC8I	GCNNGC	4 cut(s)	415, 1162, 1285, 1538
BstDEI	CTNAG	8 cut(s)	24, 255, 326, 691, 1468, 1909, 2123, 2629
BstF5I	GGATG	5 cut(s)	1597, 2215, 2356, 2461, 2578
BstHHI	GCGC	2 cut(s)	425, 1188
BstKTI	GATC	8 cut(s)	895, 1220, 1588, 1632, 2060, 2151, 2287, 2539
BstMAI	GTCTC	3 cut(s)	456, 944, 1912
BstMBI	GATC	8 cut(s)	892, 1217, 1585, 1629, 2057, 2148, 2284, 2536
BstMWI	GCNNNNNNNGC	5 cut(s)	198, 410, 1383, 1829, 1878
BstNI	CCWGG	4 cut(s)	408, 663, 1845, 2660
BstSCI	CCNGG	4 cut(s)	406, 661, 1843, 2658
BstSFI	CTRYAG	1 cut(s)	1473
BstV1I	GCAGC	3 cut(s)	110, 1386, 1459
BstV2I	GAAGAC	4 cut(s)	179, 330, 675, 1435
BstX2I	RGATCY	2 cut(s)	2057, 2284
BstXI	CCANNNNNNTGG	1 cut(s)	1649
BstYI	RGATCY	2 cut(s)	2057, 2284
Bsu36I	CCTNAGG	1 cut(s)	326
BsuI	GTATCC	2 cut(s)	497, 2477
BsuRI	GGCC	6 cut(s)	730, 991, 1045, 2178, 2437, 2616
BtsCI	GGATG	5 cut(s)	1597, 2215, 2356, 2461, 2578
BtsIMutI	CAGTG	8 cut(s)	425, 613, 1046, 1117, 1249, 1335, 1530, 1555
BveI	ACCTGC	1 cut(s)	1069
Cac8I	GCNNGC	4 cut(s)	415, 1162, 1285, 1538
CfoI	GCGC	2 cut(s)	425, 1188
Cfr10I	RCCGGY	2 cut(s)	672, 1712
Cfr13I	GGNCC	5 cut(s)	289, 728, 737, 1043, 2435
CseI	GACGC	3 cut(s)	190, 1097, 1763
CspAI	ACCGGT	2 cut(s)	672, 1712
CspCI	CAANNNNNGTGG	2 cut(s)	1059, 1094
DdeI	CTNAG	8 cut(s)	24, 255, 326, 691, 1468, 1909, 2123, 2629
DpnI	GATC	8 cut(s)	894, 1219, 1587, 1631, 2059, 2150, 2286, 2538
DpnII	GATC	8 cut(s)	892, 1217, 1585, 1629, 2057, 2148, 2284, 2536
DraI	TTTAAA	2 cut(s)	916, 1576
DraIII	CACNNNGTG	1 cut(s)	2398
DrdI	GACNNNNNNGTC	1 cut(s)	200
DriI	GACNNNNNGTC	1 cut(s)	1439
DseDI	GACNNNNNNGTC	1 cut(s)	200
EaeI	YGGCCR	1 cut(s)	2176
Eam1104I	CTCTTC	1 cut(s)	2445
Eam1105I	GACNNNNNGTC	1 cut(s)	1439
EarI	CTCTTC	1 cut(s)	2445
EciI	GGCGGA	2 cut(s)	118, 1887
Eco24I	GRGCYC	1 cut(s)	406
Eco31I	GGTCTC	1 cut(s)	456
Eco47I	GGWCC	2 cut(s)	289, 737
Eco57I	CTGAAG	1 cut(s)	1845
Eco81I	CCTNAGG	1 cut(s)	326
EcoO109I	RGGNCCY	2 cut(s)	289, 2435
EcoRII	CCWGG	4 cut(s)	406, 661, 1843, 2658
EcoT38I	GRGCYC	1 cut(s)	406
FalI	AAGNNNNNCTT	2 cut(s)	917, 949
FaqI	GGGAC	3 cut(s)	1165, 1557, 1766
FauI	CCCGC	2 cut(s)	828, 1533
FbaI	TGATCA	1 cut(s)	2148
FblI	GTMKAC	1 cut(s)	204
Fnu4HI	GCNGC	3 cut(s)	124, 1375, 1473
FokI	GGATG	5 cut(s)	1604, 2202, 2363, 2448, 2585
FriOI	GRGCYC	1 cut(s)	406
Fsp4HI	GCNGC	3 cut(s)	124, 1375, 1473
FspBI	CTAG	5 cut(s)	825, 1038, 2586, 2598, 2646
FspI	TGCGCA	2 cut(s)	424, 1187
GlaI	GCGC	2 cut(s)	424, 1187
GluI	GCNGC	3 cut(s)	124, 1375, 1473
GsaI	CCCAGC	1 cut(s)	2388
GsuI	CTGGAG	4 cut(s)	390, 684, 2070, 2529
HaeIII	GGCC	6 cut(s)	730, 991, 1045, 2178, 2437, 2616
HapII	CCGG	2 cut(s)	673, 1713
HgaI	GACGC	3 cut(s)	190, 1097, 1763
HhaI	GCGC	2 cut(s)	425, 1188
Hin1I	GRCGYC	1 cut(s)	201
Hin6I	GCGC	2 cut(s)	423, 1186
HinP1I	GCGC	2 cut(s)	423, 1186
HincII	GTYRAC	2 cut(s)	140, 205
HindII	GTYRAC	2 cut(s)	140, 205
HindIII	AAGCTT	2 cut(s)	279, 1104
HinfI	GANTC	2 cut(s)	87, 2471
HpaII	CCGG	2 cut(s)	673, 1713
HphI	GGTGA	7 cut(s)	175, 446, 614, 1595, 1727, 2093, 2384
Hpy166II	GTNNAC	7 cut(s)	30, 140, 205, 1710, 1856, 2322, 2665
Hpy188I	TCNGA	6 cut(s)	1222, 1912, 2126, 2153, 2621, 2679
Hpy8I	GTNNAC	7 cut(s)	30, 140, 205, 1710, 1856, 2322, 2665
Hpy99I	CGWCG	2 cut(s)	191, 206
HpyCH4III	ACNGT	5 cut(s)	467, 573, 608, 1339, 2076
HpyCH4IV	ACGT	1 cut(s)	955
HpyF10VI	GCNNNNNNNGC	5 cut(s)	198, 410, 1383, 1829, 1878
HpyF3I	CTNAG	8 cut(s)	24, 255, 326, 691, 1468, 1909, 2123, 2629
HpySE526I	ACGT	1 cut(s)	955
Hsp92I	GRCGYC	1 cut(s)	201
HspAI	GCGC	2 cut(s)	423, 1186
KpnI	GGTACC	1 cut(s)	1566
Ksp22I	TGATCA	1 cut(s)	2148
Kzo9I	GATC	8 cut(s)	892, 1217, 1585, 1629, 2057, 2148, 2284, 2536
LmnI	GCTCC	4 cut(s)	131, 409, 1008, 1956
Lsp1109I	GCAGC	3 cut(s)	110, 1386, 1459
LweI	GCATC	6 cut(s)	373, 763, 1819, 2001, 2224, 2317
MaeI	CTAG	5 cut(s)	825, 1038, 2586, 2598, 2646
MaeII	ACGT	1 cut(s)	955
MaeIII	GTNAC	4 cut(s)	602, 613, 827, 1715
MalI	GATC	8 cut(s)	894, 1219, 1587, 1631, 2059, 2150, 2286, 2538
MboI	GATC	8 cut(s)	892, 1217, 1585, 1629, 2057, 2148, 2284, 2536
MfeI	CAATTG	1 cut(s)	2169
MflI	RGATCY	2 cut(s)	2057, 2284
MhlI	GDGCHC	3 cut(s)	406, 415, 1323
MmeI	TCCRAC	4 cut(s)	1468, 2328, 2489, 2657
MroXI	GAANNNNTTC	1 cut(s)	1788
MslI	CAYNNNNRTG	3 cut(s)	813, 1147, 2400
MspA1I	CMGCKG	2 cut(s)	1303, 1472
MspI	CCGG	2 cut(s)	673, 1713
MspR9I	CCNGG	4 cut(s)	408, 663, 1845, 2660
MunI	CAATTG	1 cut(s)	2169
Mva1269I	GAATGC	3 cut(s)	996, 1162, 2388
MvaI	CCWGG	4 cut(s)	408, 663, 1845, 2660
MwoI	GCNNNNNNNGC	5 cut(s)	198, 410, 1383, 1829, 1878
NdeII	GATC	8 cut(s)	892, 1217, 1585, 1629, 2057, 2148, 2284, 2536
NlaIV	GGNNCC	5 cut(s)	405, 660, 1083, 1125, 1564
NmuCI	GTSAC	2 cut(s)	602, 1715
NsbI	TGCGCA	2 cut(s)	424, 1187
PctI	GAATGC	3 cut(s)	996, 1162, 2388
PdmI	GAANNNNTTC	1 cut(s)	1788
PfeI	GAWTC	2 cut(s)	87, 2471
PinAI	ACCGGT	2 cut(s)	672, 1712
PkrI	GCNGC	3 cut(s)	125, 1376, 1474
Ppu21I	YACGTR	1 cut(s)	956
PpuMI	RGGWCCY	1 cut(s)	289
PshBI	ATTAAT	2 cut(s)	1982, 2156
Psp5II	RGGWCCY	1 cut(s)	289
Psp6I	CCWGG	4 cut(s)	406, 661, 1843, 2658
PspFI	CCCAGC	1 cut(s)	2384
PspGI	CCWGG	4 cut(s)	406, 661, 1843, 2658
PspN4I	GGNNCC	5 cut(s)	405, 660, 1083, 1125, 1564
PspPI	GGNCC	5 cut(s)	289, 728, 737, 1043, 2435
PspPPI	RGGWCCY	1 cut(s)	289
PstI	CTGCAG	1 cut(s)	1477
PsuI	RGATCY	2 cut(s)	2057, 2284
PvuII	CAGCTG	2 cut(s)	1303, 1472
RseI	CAYNNNNRTG	3 cut(s)	813, 1147, 2400
SalI	GTCGAC	1 cut(s)	203
SatI	GCNGC	3 cut(s)	124, 1375, 1473
Sau3AI	GATC	8 cut(s)	892, 1217, 1585, 1629, 2057, 2148, 2284, 2536
Sau96I	GGNCC	5 cut(s)	289, 728, 737, 1043, 2435
ScaI	AGTACT	1 cut(s)	1730
ScrFI	CCNGG	4 cut(s)	408, 663, 1845, 2660
SduI	GDGCHC	3 cut(s)	406, 415, 1323
SfaNI	GCATC	6 cut(s)	373, 763, 1819, 2001, 2224, 2317
SfcI	CTRYAG	1 cut(s)	1473
SinI	GGWCC	2 cut(s)	289, 737
SmiMI	CAYNNNNRTG	3 cut(s)	813, 1147, 2400
SmlI	CTYRAG	2 cut(s)	1866, 1931
SmoI	CTYRAG	2 cut(s)	1866, 1931
SsiI	CCGC	6 cut(s)	129, 688, 821, 1405, 1540, 1872
SspI	AATATT	2 cut(s)	306, 2582
SspMI	CTAG	5 cut(s)	825, 1038, 2586, 2598, 2646
StyD4I	CCNGG	4 cut(s)	406, 661, 1843, 2658
TaaI	ACNGT	5 cut(s)	467, 573, 608, 1339, 2076
TaiI	ACGT	1 cut(s)	958
TaqI	TCGA	5 cut(s)	204, 367, 948, 1613, 2328
TatI	WGTACW	6 cut(s)	561, 886, 1014, 1728, 1976, 2524
TfiI	GAWTC	2 cut(s)	87, 2471
TscAI	CASTG	8 cut(s)	432, 613, 1053, 1124, 1249, 1342, 1537, 1562
TseFI	GTSAC	2 cut(s)	602, 1715
TseI	GCWGC	3 cut(s)	123, 1374, 1472
Tsp45I	GTSAC	2 cut(s)	602, 1715
TspGWI	ACGGA	4 cut(s)	254, 1649, 2549, 2565
TspRI	CASTG	8 cut(s)	432, 613, 1053, 1124, 1249, 1342, 1537, 1562
VpaK11BI	GGWCC	2 cut(s)	289, 737
VspI	ATTAAT	2 cut(s)	1982, 2156
XapI	RAATTY	4 cut(s)	227, 911, 1615, 2207
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1950, 2045, 2609
XmiI	GTMKAC	1 cut(s)	204
XmnI	GAANNNNTTC	1 cut(s)	1788
XspI	CTAG	5 cut(s)	825, 1038, 2586, 2598, 2646
ZrmI	AGTACT	1 cut(s)	1730

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.