Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0003672

Physical Location & Seq

Genomic Coordinates Forward (+)

34327420 .. 34336797

Gene Structure 9378 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG311200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2694 bp

ATGGCTTCACTTTCTCTTTCAACCAAGTTCCCTCTGTACCCCAACCTCACATTTCTCCAATTCCAGTACCCAAACAGACCTCAAAGAATCACCTTTCCTATCAAGAAGAAAACCCAGATAGGCTGCTCTGCCACAGGTCAACCCCACCAACAACAAAGACCCCCCAAGAAGAAGAAAACCCTCACCGACGGCGACAAAGGCGTCGACCCAGTTGGCTTCCTCTCCAAATTCGACATCTCCCATAAGCAATTTGCTCAGTTCCTCCGTGAAAGACACAAAGCCCTCAAGGACCTGACAGATGAAATATTAACTCGCCACATCAACCTCAGGGATATGTCTTCTGGATATGAGATATTGGGTTTGCATCGACACCCAGAACATAGAGAGGATTATATGGAGTGGGCTCCAGGTGCTCGCTACTGCGCACTGGTTGGTGATTTCAATGGGTGGTCTCCCACAGAGAACTGTGCAAAAGAGGGTCATTTTGGACATGATGACTATGGATACTGGTTCATCATTCTTGAAGATAAGTTGAGGAATGGACAGAAACCAGATGAGCTGTACTTTCAACAGTATAACTATGTAGATGATTACGATAAAGGTGACAGTGGTGTTACTATTGAAGAAGTCTTTAAGAAAGCAAATGATGAGTATTGGGAACCTGGAGAAGACCGGTTTCTAAAAAACCGCTTAGAAGTACCAGCAAAGTTGTATGAGCAGATTTTTGGGCCTAATGGACCTCAAACGTTAGAGGAACTTGAAGAAATACCAGATGCAGAAACAAGATATAAAGCGTGGAAAGAACAGCATAAAGATGACCCGCCTAGTAACTTACCTTCTTATGATGTGATTGACAATGGAAAAGAGTATGACATTTACAATGTTGTACTTGATCCAGAATCACAAGAGAAATTTAAAGCAAAGAAGCCTCCCTTGCCATATTGGTTCGAAATACGTAAAGGAAGGAAGGCATGGTTGAAAAAGTATAGGCCGTGCATTCCTCATGGGAGCAAGTACAGGGTCTATTTTAATACTCCTAGTGGGCCACTGGAACGAGTACCTGCTTGGGCAACTTATGTGGAACCAGATGCAGATGGAGGACAAGCTTTTGCAATCCACTGGGAACCACCCCCAGAACTTGCATACAAATGGAAGAATGCACGCCCTAAAGTGCCAAAGTCCCTGCGCATTTATGAATGTCATGTTGGCATAAGTGGATCAGAGCCTAAAGTATCTTCTTTCAGTGATTTCACAGAGAAGGTTCTTCCTCATGTAAAGGAGGCTGGCTATAATGCAATTCAGCTGATTGGAGTTATTGAGCACAAAGATTATTTCACTGTTGGTTATAGAGCCACTAATTTATATGCTGTTAGCAGCCGATTTGGCACTCCTGATGATTTCAAGCGGTTGGTTGATGAAGCACATGGACAAGGACTGCTGGTCTTCTTGGAGATTGTCCATTCCTACTCAGCTGCAGATGAGATGGTTGGATTAGGACTCTTTGATGGAACAAATGACTGCTACTTCCACACTGGCAAACGGGGACATCACAAGCACTGGGGTACCAGAATGTTTAAATATGGTGATCTGGATGTTCTGCATTTCCTTCTCTCGAATTTGAACTGGTGGATCACAGAGTACCAAGTTGATGGTTTTCAGTTCCATTCACTCTCATCAATGATTTATACTCATAATGGTTTTGCTTCGTTTACCGGTGACTTGGAGGAGTACTGCAATCAATATGTTGATAGGGACGCATTGTTATACCTCATTTTGGCAAACGAAATACTTCATGCTCTCCACCCAGATATAATAACGATTGCTGAAGATGCAACCTTCTATCCAGGTCTATGTGAACCAATCTCTCAAGGCGGATTGGGCTTTGATTATTGTGTCAATCTTTCTGTCTCAGAGATGTGGTCATCTTTCCTTGAGAATGTTCCAGACCATGATTGGAGCATGAGTAAGATTGTGAGTACATTAATGGGCAACAAAAATTATGCAGATAAGATGCTCATGTATGCTGAAAATCATAACCAGTCAATTTCTGGAGGGAGATCTTTTGCAGAGATACTGTTTGGTGAAATCAACGAGGGCTCTCCTGACAAGGAGAAATTATTGCTCAGAGGTTGTTCCTTGCACAAGATGATCAGATTAATTACCATGACAATTGGTGGCCGTGCTTATCTCAACTTCATGGGCAATGAATTTGGGCATCCAGAGAGGGTTGAGTTCCCAATGCCAAGCAACAATTTCTCATTTTCTCTTGCCAACCGAAGGTGGGATCTTCTTGCCAACGAGGGATTGCATCTCAACTTGTTTACCTTCGATAAGGACTTGATGAAGTTGGATGAAAATGAAAGAATACTTCCAAGAGTTTTACCTAGCATTCACCATGTGAATGACAATAGCATGGTAATTGCTTACATAAGAGGGCCTCTTCTATTCGTATTTAACTTTCATCCAACAGATTCTTATGAAGGATACCGAATAGGTGTAGAAGAAGCTGGAGAGTATCAACTTGTACTAAATACGGATGAAAGGAAGTACGGAGGTCAAGGGCTTTTGAAGGATGACCAATATTCTAGGACAACTACTAGCCAAAGAAGTGATGGCCTCCGAAACAGCTTAGAAGTTCCTCTGCCTAGTAGAACTGCCCAGGTTTACAAATTAAGTCGGATTTTAAGAATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0007275 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009791 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0048856 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

897

Amino Acids

103.34

Weight (kDa)

5.92

Isoelectric Point (pI)

35.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Alpha-amylase	PF00128	422 - 490	1.1e-06	Alpha amylase, catalytic domain
Alpha-amylase_C	PF02806	799 - 891	4.5e-22	Alpha amylase, C-terminal all-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003672)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20440 AT3G20440 AT3G20440
fragaria_vesca	FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220
malus_domestica	MD11G1212700.v1.1
prunus_persica	Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1
pyrus_communis	pycom11g18770
rosa_chinensis	RchiOBHm_Chr5g0040901 RchiOBHm_Chr5g0040911
rosa_laevigata	RLG00000034012
rosa_multiflora	Rmu_sc0002848.1_g000016 Rmu_ssc0000416.1_g000003
rosa_roxburghii	Rroxscaffold_1G00039790
rosa_rugosa	Rorug05G0188500 Rorug05G0188600 Rorug05G0188600
rosa_samantha	Rh5AG274100 Rh5BG279100 Rh5CG311200 Rh5DG287300
rosa_wichuraiana	Rw5G025750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	200
Acc16I	TGCGCA	2 cut(s)	424, 1187
Acc36I	ACCTGC	1 cut(s)	1069
Acc65I	GGTACC	1 cut(s)	1562
AccB1I	GGYRCC	1 cut(s)	1562
AccI	GTMKAC	1 cut(s)	204
AciI	CCGC	4 cut(s)	688, 821, 1405, 1872
AclI	AACGTT	1 cut(s)	746
AclWI	GGATC	4 cut(s)	887, 1225, 1637, 2292
AcoI	YGGCCR	1 cut(s)	2176
AcsI	RAATTY	4 cut(s)	227, 911, 1615, 2207
AcuI	CTGAAG	1 cut(s)	1845
AcyI	GRCGYC	1 cut(s)	201
AdeI	CACNNNGTG	1 cut(s)	2398
AfiI	CCNNNNNNNGG	3 cut(s)	1774, 2277, 2281
AgeI	ACCGGT	2 cut(s)	672, 1712
AhdI	GACNNNNNGTC	1 cut(s)	1439
AjnI	CCWGG	4 cut(s)	406, 661, 1843, 2658
AjuI	GAANNNNNNNTTGG	2 cut(s)	1188, 1220
AluBI	AGCT	6 cut(s)	559, 1106, 1303, 1472, 2507, 2628
AluI	AGCT	6 cut(s)	559, 1106, 1303, 1472, 2507, 2628
Alw21I	GWGCWC	2 cut(s)	415, 1323
Alw26I	GTCTC	2 cut(s)	456, 1912
AlwI	GGATC	4 cut(s)	887, 1225, 1637, 2292
AoxI	GGCC	6 cut(s)	728, 989, 1043, 2176, 2435, 2614
ApeKI	GCWGC	3 cut(s)	123, 1374, 1472
ApoI	RAATTY	4 cut(s)	227, 911, 1615, 2207
AseI	ATTAAT	2 cut(s)	1982, 2156
AsiGI	ACCGGT	2 cut(s)	672, 1712
Asp700I	GAANNNNTTC	1 cut(s)	1788
Asp718I	GGTACC	1 cut(s)	1562
AspLEI	GCGC	2 cut(s)	425, 1188
AspS9I	GGNCC	5 cut(s)	289, 728, 737, 1043, 2435
AsuHPI	GGTGA	8 cut(s)	82, 175, 446, 614, 1595, 1727, 2093, 2384
AsuII	TTCGAA	1 cut(s)	948
AvaII	GGWCC	2 cut(s)	289, 737
AxyI	CCTNAGG	1 cut(s)	326
BaeI	ACNNNNGTAYC	2 cut(s)	1546, 1579
BanI	GGYRCC	1 cut(s)	1562
BanII	GRGCYC	2 cut(s)	406, 2099
BarI	GAAGNNNNNNTAC	2 cut(s)	820, 852
BbsI	GAAGAC	3 cut(s)	330, 675, 1435
Bbv12I	GWGCWC	2 cut(s)	415, 1323
BbvI	GCAGC	3 cut(s)	110, 1386, 1459
BccI	CCATC	5 cut(s)	1088, 1477, 1499, 1643, 2606
BceAI	ACGGC	3 cut(s)	205, 976, 2163
BciT130I	CCWGG	4 cut(s)	408, 663, 1845, 2660
BciVI	GTATCC	2 cut(s)	497, 2477
BclI	TGATCA	1 cut(s)	2148
BcoDI	GTCTC	2 cut(s)	456, 1912
BfaI	CTAG	6 cut(s)	825, 1038, 2385, 2586, 2598, 2646
BfmI	CTRYAG	1 cut(s)	1473
BfuAI	ACCTGC	1 cut(s)	1069
BfuI	GTATCC	2 cut(s)	497, 2477
BglI	GCCNNNNNGGC	1 cut(s)	1383
BglII	AGATCT	1 cut(s)	2057
BisI	GCNGC	3 cut(s)	124, 1375, 1473
BlsI	GCNGC	3 cut(s)	125, 1376, 1474
BmcAI	AGTACT	1 cut(s)	1730
Bme1390I	CCNGG	4 cut(s)	408, 663, 1845, 2660
Bme18I	GGWCC	2 cut(s)	289, 737
BmeRI	GACNNNNNGTC	1 cut(s)	1439
BmgT120I	GGNCC	5 cut(s)	289, 728, 737, 1043, 2435
BmiI	GGNNCC	5 cut(s)	405, 660, 1083, 1125, 1564
BmrFI	CCNGG	4 cut(s)	408, 663, 1845, 2660
BmrI	ACTGGG	3 cut(s)	203, 1129, 1567
BmsI	GCATC	7 cut(s)	373, 763, 1078, 1819, 2001, 2224, 2317
BmuI	ACTGGG	3 cut(s)	203, 1129, 1567
BpiI	GAAGAC	3 cut(s)	330, 675, 1435
BpmI	CTGGAG	4 cut(s)	390, 684, 2070, 2529
Bpu14I	TTCGAA	1 cut(s)	948
BpuEI	CTTGAG	3 cut(s)	269, 1851, 1952
BsaAI	YACGTR	1 cut(s)	956
BsaHI	GRCGYC	1 cut(s)	201
BsaI	GGTCTC	1 cut(s)	456
BsaJI	CCNNGG	1 cut(s)	2658
BsaWI	WCCGGW	2 cut(s)	672, 1712
BsaXI	ACNNNNNCTCC	2 cut(s)	1948, 1978
Bsc4I	CCNNNNNNNGG	3 cut(s)	1774, 2277, 2281
Bse118I	RCCGGY	2 cut(s)	672, 1712
Bse21I	CCTNAGG	1 cut(s)	326
Bse3DI	GCAATG	1 cut(s)	2209
BseBI	CCWGG	4 cut(s)	408, 663, 1845, 2660
BseDI	CCNNGG	1 cut(s)	2658
BseGI	GGATG	6 cut(s)	1597, 2215, 2356, 2461, 2542, 2578
BseLI	CCNNNNNNNGG	3 cut(s)	1774, 2277, 2281
BseMI	GCAATG	1 cut(s)	2209
BseMII	CTCAG	5 cut(s)	269, 340, 1482, 1923, 2137
BseRI	GAGGAG	1 cut(s)	1739
BseXI	GCAGC	3 cut(s)	110, 1386, 1459
BshFI	GGCC	6 cut(s)	730, 991, 1045, 2178, 2437, 2616
BshNI	GGYRCC	1 cut(s)	1562
BshTI	ACCGGT	2 cut(s)	672, 1712
BsiHKAI	GWGCWC	2 cut(s)	415, 1323
BsiSI	CCGG	2 cut(s)	673, 1713
BslFI	GGGAC	3 cut(s)	1165, 1557, 1766
BslI	CCNNNNNNNGG	3 cut(s)	1774, 2277, 2281
BsmAI	GTCTC	2 cut(s)	456, 1912
BsmFI	GGGAC	3 cut(s)	1165, 1557, 1766
BsmI	GAATGC	3 cut(s)	996, 1162, 2388
BsnI	GGCC	6 cut(s)	730, 991, 1045, 2178, 2437, 2616
Bso31I	GGTCTC	1 cut(s)	456
Bsp119I	TTCGAA	1 cut(s)	948
Bsp1286I	GDGCHC	4 cut(s)	406, 415, 1323, 2099
Bsp143I	GATC	7 cut(s)	892, 1217, 1585, 1629, 2057, 2148, 2284
BspACI	CCGC	4 cut(s)	688, 821, 1405, 1872
BspANI	GGCC	6 cut(s)	730, 991, 1045, 2178, 2437, 2616
BspCNI	CTCAG	5 cut(s)	268, 339, 1481, 1922, 2136
BspLI	GGNNCC	5 cut(s)	405, 660, 1083, 1125, 1564
BspMAI	CTGCAG	1 cut(s)	1477
BspMI	ACCTGC	1 cut(s)	1069
BspPI	GGATC	4 cut(s)	887, 1225, 1637, 2292
BspT104I	TTCGAA	1 cut(s)	948
BspT107I	GGYRCC	1 cut(s)	1562
BspTNI	GGTCTC	1 cut(s)	456
BsrDI	GCAATG	1 cut(s)	2209
BsrFI	RCCGGY	2 cut(s)	672, 1712
BssAI	RCCGGY	2 cut(s)	672, 1712
BssECI	CCNNGG	1 cut(s)	2658
BssMI	GATC	7 cut(s)	892, 1217, 1585, 1629, 2057, 2148, 2284
BssNI	GRCGYC	1 cut(s)	201
Bst2UI	CCWGG	4 cut(s)	408, 663, 1845, 2660
Bst4CI	ACNGT	5 cut(s)	467, 573, 608, 1339, 2076
Bst6I	CTCTTC	1 cut(s)	2445
BstACI	GRCGYC	1 cut(s)	201
BstBAI	YACGTR	1 cut(s)	956
BstBI	TTCGAA	1 cut(s)	948
BstC8I	GCNNGC	3 cut(s)	415, 1162, 1285
BstDEI	CTNAG	7 cut(s)	255, 326, 691, 1468, 1909, 2123, 2629
BstF5I	GGATG	6 cut(s)	1597, 2215, 2356, 2461, 2542, 2578
BstHHI	GCGC	2 cut(s)	425, 1188
BstKTI	GATC	7 cut(s)	895, 1220, 1588, 1632, 2060, 2151, 2287
BstMAI	GTCTC	2 cut(s)	456, 1912
BstMBI	GATC	7 cut(s)	892, 1217, 1585, 1629, 2057, 2148, 2284
BstMWI	GCNNNNNNNGC	6 cut(s)	198, 410, 934, 1383, 1829, 1878
BstNI	CCWGG	4 cut(s)	408, 663, 1845, 2660
BstSCI	CCNGG	4 cut(s)	406, 661, 1843, 2658
BstSFI	CTRYAG	1 cut(s)	1473
BstSNI	TACGTA	1 cut(s)	956
BstV1I	GCAGC	3 cut(s)	110, 1386, 1459
BstV2I	GAAGAC	3 cut(s)	330, 675, 1435
BstX2I	RGATCY	2 cut(s)	2057, 2284
BstXI	CCANNNNNNTGG	1 cut(s)	1649
BstYI	RGATCY	2 cut(s)	2057, 2284
Bsu36I	CCTNAGG	1 cut(s)	326
BsuI	GTATCC	2 cut(s)	497, 2477
BsuRI	GGCC	6 cut(s)	730, 991, 1045, 2178, 2437, 2616
BtsCI	GGATG	6 cut(s)	1597, 2215, 2356, 2461, 2542, 2578
BtsIMutI	CAGTG	8 cut(s)	425, 613, 1046, 1117, 1249, 1335, 1530, 1555
BveI	ACCTGC	1 cut(s)	1069
Cac8I	GCNNGC	3 cut(s)	415, 1162, 1285
CfoI	GCGC	2 cut(s)	425, 1188
Cfr10I	RCCGGY	2 cut(s)	672, 1712
Cfr13I	GGNCC	5 cut(s)	289, 728, 737, 1043, 2435
CseI	GACGC	2 cut(s)	190, 1763
CspAI	ACCGGT	2 cut(s)	672, 1712
CspCI	CAANNNNNGTGG	2 cut(s)	1059, 1094
DdeI	CTNAG	7 cut(s)	255, 326, 691, 1468, 1909, 2123, 2629
DpnI	GATC	7 cut(s)	894, 1219, 1587, 1631, 2059, 2150, 2286
DpnII	GATC	7 cut(s)	892, 1217, 1585, 1629, 2057, 2148, 2284
DraI	TTTAAA	2 cut(s)	916, 1576
DraIII	CACNNNGTG	1 cut(s)	2398
DrdI	GACNNNNNNGTC	1 cut(s)	200
DriI	GACNNNNNGTC	1 cut(s)	1439
DseDI	GACNNNNNNGTC	1 cut(s)	200
EaeI	YGGCCR	1 cut(s)	2176
Eam1104I	CTCTTC	1 cut(s)	2445
Eam1105I	GACNNNNNGTC	1 cut(s)	1439
EarI	CTCTTC	1 cut(s)	2445
EciI	GGCGGA	1 cut(s)	1887
Eco105I	TACGTA	1 cut(s)	956
Eco24I	GRGCYC	2 cut(s)	406, 2099
Eco31I	GGTCTC	1 cut(s)	456
Eco47I	GGWCC	2 cut(s)	289, 737
Eco57I	CTGAAG	1 cut(s)	1845
Eco81I	CCTNAGG	1 cut(s)	326
EcoO109I	RGGNCCY	2 cut(s)	289, 2435
EcoRII	CCWGG	4 cut(s)	406, 661, 1843, 2658
EcoT38I	GRGCYC	2 cut(s)	406, 2099
FalI	AAGNNNNNCTT	2 cut(s)	917, 949
FaqI	GGGAC	3 cut(s)	1165, 1557, 1766
FauI	CCCGC	1 cut(s)	828
FbaI	TGATCA	1 cut(s)	2148
FblI	GTMKAC	1 cut(s)	204
Fnu4HI	GCNGC	3 cut(s)	124, 1375, 1473
FokI	GGATG	6 cut(s)	1604, 2202, 2363, 2448, 2549, 2585
FriOI	GRGCYC	2 cut(s)	406, 2099
Fsp4HI	GCNGC	3 cut(s)	124, 1375, 1473
FspBI	CTAG	6 cut(s)	825, 1038, 2385, 2586, 2598, 2646
FspI	TGCGCA	2 cut(s)	424, 1187
GlaI	GCGC	2 cut(s)	424, 1187
GluI	GCNGC	3 cut(s)	124, 1375, 1473
GsuI	CTGGAG	4 cut(s)	390, 684, 2070, 2529
HaeIII	GGCC	6 cut(s)	730, 991, 1045, 2178, 2437, 2616
HapII	CCGG	2 cut(s)	673, 1713
HgaI	GACGC	2 cut(s)	190, 1763
HhaI	GCGC	2 cut(s)	425, 1188
Hin1I	GRCGYC	1 cut(s)	201
Hin6I	GCGC	2 cut(s)	423, 1186
HinP1I	GCGC	2 cut(s)	423, 1186
HincII	GTYRAC	2 cut(s)	140, 205
HindII	GTYRAC	2 cut(s)	140, 205
HindIII	AAGCTT	1 cut(s)	1104
HinfI	GANTC	4 cut(s)	87, 899, 1497, 2471
HpaII	CCGG	2 cut(s)	673, 1713
HphI	GGTGA	8 cut(s)	82, 175, 446, 614, 1595, 1727, 2093, 2384
Hpy166II	GTNNAC	6 cut(s)	140, 205, 1710, 1856, 2322, 2665
Hpy188I	TCNGA	6 cut(s)	1222, 1912, 2126, 2153, 2621, 2679
Hpy8I	GTNNAC	6 cut(s)	140, 205, 1710, 1856, 2322, 2665
Hpy99I	CGWCG	2 cut(s)	191, 206
HpyCH4III	ACNGT	5 cut(s)	467, 573, 608, 1339, 2076
HpyCH4IV	ACGT	2 cut(s)	746, 955
HpyF10VI	GCNNNNNNNGC	6 cut(s)	198, 410, 934, 1383, 1829, 1878
HpyF3I	CTNAG	7 cut(s)	255, 326, 691, 1468, 1909, 2123, 2629
HpySE526I	ACGT	2 cut(s)	746, 955
Hsp92I	GRCGYC	1 cut(s)	201
HspAI	GCGC	2 cut(s)	423, 1186
KpnI	GGTACC	1 cut(s)	1566
Ksp22I	TGATCA	1 cut(s)	2148
Kzo9I	GATC	7 cut(s)	892, 1217, 1585, 1629, 2057, 2148, 2284
LmnI	GCTCC	3 cut(s)	409, 1008, 1956
Lsp1109I	GCAGC	3 cut(s)	110, 1386, 1459
LweI	GCATC	7 cut(s)	373, 763, 1078, 1819, 2001, 2224, 2317
MaeI	CTAG	6 cut(s)	825, 1038, 2385, 2586, 2598, 2646
MaeII	ACGT	2 cut(s)	746, 955
MaeIII	GTNAC	4 cut(s)	602, 613, 827, 1715
MalI	GATC	7 cut(s)	894, 1219, 1587, 1631, 2059, 2150, 2286
MboI	GATC	7 cut(s)	892, 1217, 1585, 1629, 2057, 2148, 2284
MfeI	CAATTG	1 cut(s)	2169
MflI	RGATCY	2 cut(s)	2057, 2284
MhlI	GDGCHC	4 cut(s)	406, 415, 1323, 2099
MlyI	GAGTC	1 cut(s)	1491
MmeI	TCCRAC	4 cut(s)	1468, 2328, 2489, 2657
MroXI	GAANNNNTTC	1 cut(s)	1788
MslI	CAYNNNNRTG	3 cut(s)	813, 1147, 2400
MspA1I	CMGCKG	2 cut(s)	1303, 1472
MspI	CCGG	2 cut(s)	673, 1713
MspR9I	CCNGG	4 cut(s)	408, 663, 1845, 2660
MunI	CAATTG	1 cut(s)	2169
Mva1269I	GAATGC	3 cut(s)	996, 1162, 2388
MvaI	CCWGG	4 cut(s)	408, 663, 1845, 2660
MwoI	GCNNNNNNNGC	6 cut(s)	198, 410, 934, 1383, 1829, 1878
NdeII	GATC	7 cut(s)	892, 1217, 1585, 1629, 2057, 2148, 2284
NlaIV	GGNNCC	5 cut(s)	405, 660, 1083, 1125, 1564
NmuCI	GTSAC	2 cut(s)	602, 1715
NsbI	TGCGCA	2 cut(s)	424, 1187
NspV	TTCGAA	1 cut(s)	948
PctI	GAATGC	3 cut(s)	996, 1162, 2388
PdmI	GAANNNNTTC	1 cut(s)	1788
PfeI	GAWTC	3 cut(s)	87, 899, 2471
PinAI	ACCGGT	2 cut(s)	672, 1712
PkrI	GCNGC	3 cut(s)	125, 1376, 1474
PleI	GAGTC	1 cut(s)	1491
PpsI	GAGTC	1 cut(s)	1491
Ppu21I	YACGTR	1 cut(s)	956
PpuMI	RGGWCCY	1 cut(s)	289
PshBI	ATTAAT	2 cut(s)	1982, 2156
Psp1406I	AACGTT	1 cut(s)	746
Psp5II	RGGWCCY	1 cut(s)	289
Psp6I	CCWGG	4 cut(s)	406, 661, 1843, 2658
PspGI	CCWGG	4 cut(s)	406, 661, 1843, 2658
PspN4I	GGNNCC	5 cut(s)	405, 660, 1083, 1125, 1564
PspPI	GGNCC	5 cut(s)	289, 728, 737, 1043, 2435
PspPPI	RGGWCCY	1 cut(s)	289
PstI	CTGCAG	1 cut(s)	1477
PsuI	RGATCY	2 cut(s)	2057, 2284
PvuII	CAGCTG	2 cut(s)	1303, 1472
RseI	CAYNNNNRTG	3 cut(s)	813, 1147, 2400
SalI	GTCGAC	1 cut(s)	203
SatI	GCNGC	3 cut(s)	124, 1375, 1473
Sau3AI	GATC	7 cut(s)	892, 1217, 1585, 1629, 2057, 2148, 2284
Sau96I	GGNCC	5 cut(s)	289, 728, 737, 1043, 2435
ScaI	AGTACT	1 cut(s)	1730
SchI	GAGTC	1 cut(s)	1491
ScrFI	CCNGG	4 cut(s)	408, 663, 1845, 2660
SduI	GDGCHC	4 cut(s)	406, 415, 1323, 2099
SfaNI	GCATC	7 cut(s)	373, 763, 1078, 1819, 2001, 2224, 2317
SfcI	CTRYAG	1 cut(s)	1473
SfuI	TTCGAA	1 cut(s)	948
SinI	GGWCC	2 cut(s)	289, 737
SmiMI	CAYNNNNRTG	3 cut(s)	813, 1147, 2400
SmlI	CTYRAG	3 cut(s)	284, 1866, 1931
SmoI	CTYRAG	3 cut(s)	284, 1866, 1931
SnaBI	TACGTA	1 cut(s)	956
SsiI	CCGC	4 cut(s)	688, 821, 1405, 1872
SspI	AATATT	2 cut(s)	306, 2582
SspMI	CTAG	6 cut(s)	825, 1038, 2385, 2586, 2598, 2646
StyD4I	CCNGG	4 cut(s)	406, 661, 1843, 2658
TaaI	ACNGT	5 cut(s)	467, 573, 608, 1339, 2076
TaiI	ACGT	2 cut(s)	749, 958
TaqI	TCGA	6 cut(s)	204, 231, 367, 948, 1613, 2328
TatI	WGTACW	6 cut(s)	561, 886, 1014, 1728, 1976, 2524
TfiI	GAWTC	3 cut(s)	87, 899, 2471
TscAI	CASTG	8 cut(s)	432, 613, 1053, 1124, 1249, 1342, 1537, 1562
TseFI	GTSAC	2 cut(s)	602, 1715
TseI	GCWGC	3 cut(s)	123, 1374, 1472
Tsp45I	GTSAC	2 cut(s)	602, 1715
TspGWI	ACGGA	3 cut(s)	254, 2549, 2565
TspRI	CASTG	8 cut(s)	432, 613, 1053, 1124, 1249, 1342, 1537, 1562
VpaK11BI	GGWCC	2 cut(s)	289, 737
VspI	ATTAAT	2 cut(s)	1982, 2156
XapI	RAATTY	4 cut(s)	227, 911, 1615, 2207
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1950, 2045, 2609
XmiI	GTMKAC	1 cut(s)	204
XmnI	GAANNNNTTC	1 cut(s)	1788
XspI	CTAG	6 cut(s)	825, 1038, 2385, 2586, 2598, 2646
ZrmI	AGTACT	1 cut(s)	1730

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.