RchiOBHm_Chr5g0040911

1,4-alpha-glucan-branching enzyme 3, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003672
Physical Location & Seq
Forward (+)
35531365 .. 35538387
7023 bp
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UTR
Exon/CDS
Intron
PRQ31938

Sequence Viewer

Length: 1365 bp
ATGTTGACACATCTATTTCCTGCCACTAATTTATATGCTGTTAGCAGCCGATTTGGCACTCCTGATGATTTCAAGCGGTTGGTTGATGAAGCACATGGACAAGGACTGCTGGTCTTCTTGGAGATTGTCCATTCCTACTCAGCTGCAGATGAGATGGTTGGATTAGGGCTTTTTGATGGAACAAATGACTGCTACTTCCACACTGGCAAACGGGGACATCACAAGCACTGGGGTACCAGAATGTTTAAATATGGTGATCTGGATGTTCTGCATTTCCTTCTCTCGAATTTGAACTGGTGGATCACGGAGTACCAAGTTGATGGTTTTCAGTTCCATTCACTTTCATCAATGATTTATACTCATAATGGTTTTGCTTCGTTTACTGGTGACTTGGAGGAGTACTGCAATCAATATGTTGATCGGGACGCATTGTTGTACCTCATTTTGGCAAACGAAATACTTCATGCTCTCCACCCAGATATAATAACGATTGCTGAAGATGCAACCTTCTATCCAGGTCTATGTGAACCAATCTCTCAAGGCGGATTGGGCTTTGATTATTGTGTCAATCTTTCTGTCTCAGAGATGTGGTCATCTTTCCTTGAGAATGTTCCAGACCATGATTGGAGCATGAGTAAGATTGTGAGTACATTAATGGGCAACAAAAATTATGCAGATAAGATGCTCATGTATGCTGAAAATCATAACCAGTCAATTTCTGGAGGGAGATCTTTTGCAGAGATACTGTTTGGTGAAATCAGGGAGGGCTCTCCTGACAAGGAGAAATTATTGCTCAGAGGTTGTTCCTTGCACAAGATGATCAAATTAATTACTATGACAATTGGTGGCCGTGCTTATCTCAACTTCATGGGCAATGAATTTGGGCATCCAGAGAGGGTTGAGTTCCCAATGCCAAGCAACAATTTCTCATTTTCTCTTGCCAACCGAAGGTGGGATCTTCTTGCCAACGAGGGATTGCATCTCAACTTGTTTAGTTTCGATAAGGACTTGATGAAGTTGGATGAAAATGAAAGAATACTTCCAAGAGTTTTACCCAGCATTCACCATGTGAATGACAATAGCATGGTAATTGCTTACATAAGAGGGCCTCTTCTATTCGTATTTAACTTTCATCCAACAGATTCTTATGAAGGATACCGAATAGGTGTAGAAGAAGCTGGAGAGTATCAACTTGTACTAAATACGGATGAAAGAAAGTACGGAGGTCAAGGGCTTTTGAAGGATGACCAATATTCTAGGACAACTACTAGCCAAAGAAGTGATGGTCTCCGAAACAGCTTAGAAGTTCCTCTGCCTAGTAGGACTGCCCAGGCTTACAAATTAAGTCGGATTTTAAGAATATAA

Protein Analysis

454

Amino Acids

51.83

Weight (kDa)

5.52

Isoelectric Point (pI)

32.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alpha-amylase_C PF02806 356 - 448 3.1e-22 Alpha amylase, C-terminal all-beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 233
AccB1I GGYRCC 1 cut(s) 233
AciI CCGC 2 cut(s) 76, 543
AclWI GGATC 2 cut(s) 308, 963
AcoI YGGCCR 1 cut(s) 847
AcsI RAATTY 2 cut(s) 286, 878
AcuI CTGAAG 1 cut(s) 516
AdeI CACNNNGTG 1 cut(s) 1069
AfaI GTAC 7 cut(s) 235, 311, 401, 437, 649, 1197, 1220
AfiI CCNNNNNNNGG 3 cut(s) 445, 948, 952
AgsI TTSAA 3 cut(s) 73, 292, 1240
AhdI GACNNNNNGTC 1 cut(s) 110
AjnI CCWGG 2 cut(s) 514, 1329
AluBI AGCT 3 cut(s) 143, 1178, 1299
AluI AGCT 3 cut(s) 143, 1178, 1299
Alw26I GTCTC 2 cut(s) 583, 1292
AlwI GGATC 2 cut(s) 308, 963
AoxI GGCC 2 cut(s) 847, 1106
ApeKI GCWGC 2 cut(s) 45, 143
ApoI RAATTY 2 cut(s) 286, 878
AseI ATTAAT 2 cut(s) 653, 827
Asp700I GAANNNNTTC 1 cut(s) 459
Asp718I GGTACC 1 cut(s) 233
AspS9I GGNCC 1 cut(s) 1106
AsuHPI GGTGA 4 cut(s) 266, 398, 764, 1055
BaeI ACNNNNGTAYC 2 cut(s) 217, 250
BanI GGYRCC 1 cut(s) 233
BanII GRGCYC 1 cut(s) 770
BbsI GAAGAC 1 cut(s) 106
BbvI GCAGC 2 cut(s) 57, 130
BccI CCATC 4 cut(s) 148, 170, 314, 1277
BceAI ACGGC 1 cut(s) 834
BciT130I CCWGG 2 cut(s) 516, 1331
BciVI GTATCC 1 cut(s) 1148
BclI TGATCA 1 cut(s) 819
BcoDI GTCTC 2 cut(s) 583, 1292
BfaI CTAG 3 cut(s) 1257, 1269, 1317
BfmI CTRYAG 1 cut(s) 144
BfuI GTATCC 1 cut(s) 1148
BglI GCCNNNNNGGC 1 cut(s) 54
BglII AGATCT 1 cut(s) 728
BisI GCNGC 2 cut(s) 46, 144
BlsI GCNGC 2 cut(s) 47, 145
BmcAI AGTACT 1 cut(s) 401
Bme1390I CCNGG 2 cut(s) 516, 1331
BmeRI GACNNNNNGTC 1 cut(s) 110
BmgT120I GGNCC 1 cut(s) 1106
BmiI GGNNCC 1 cut(s) 235
BmrFI CCNGG 2 cut(s) 516, 1331
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 4 cut(s) 490, 672, 895, 988
BmuI ACTGGG 1 cut(s) 238
BpiI GAAGAC 1 cut(s) 106
BpmI CTGGAG 2 cut(s) 741, 1200
BpuEI CTTGAG 2 cut(s) 522, 623
BsaI GGTCTC 1 cut(s) 1292
BsaJI CCNNGG 1 cut(s) 1329
BsaXI ACNNNNNCTCC 2 cut(s) 619, 649
Bsc4I CCNNNNNNNGG 3 cut(s) 445, 948, 952
Bse1I ACTGG 5 cut(s) 208, 233, 299, 388, 709
Bse3DI GCAATG 1 cut(s) 880
BseBI CCWGG 2 cut(s) 516, 1331
BseDI CCNNGG 1 cut(s) 1329
BseGI GGATG 6 cut(s) 268, 886, 1027, 1132, 1213, 1249
BseLI CCNNNNNNNGG 3 cut(s) 445, 948, 952
BseMI GCAATG 1 cut(s) 880
BseMII CTCAG 3 cut(s) 153, 594, 808
BseNI ACTGG 5 cut(s) 208, 233, 299, 388, 709
BseRI GAGGAG 1 cut(s) 410
BseXI GCAGC 2 cut(s) 57, 130
BseYI CCCAGC 1 cut(s) 1055
BshFI GGCC 2 cut(s) 849, 1108
BshNI GGYRCC 1 cut(s) 233
BslFI GGGAC 2 cut(s) 228, 437
BslI CCNNNNNNNGG 3 cut(s) 445, 948, 952
BsmAI GTCTC 2 cut(s) 583, 1292
BsmFI GGGAC 2 cut(s) 228, 437
BsmI GAATGC 1 cut(s) 1059
BsnI GGCC 2 cut(s) 849, 1108
Bso31I GGTCTC 1 cut(s) 1292
Bsp1286I GDGCHC 1 cut(s) 770
Bsp143I GATC 6 cut(s) 256, 300, 418, 728, 819, 955
BspACI CCGC 2 cut(s) 76, 543
BspANI GGCC 2 cut(s) 849, 1108
BspCNI CTCAG 3 cut(s) 152, 593, 807
BspLI GGNNCC 1 cut(s) 235
BspMAI CTGCAG 1 cut(s) 148
BspPI GGATC 2 cut(s) 308, 963
BspT107I GGYRCC 1 cut(s) 233
BspTNI GGTCTC 1 cut(s) 1292
BsrDI GCAATG 1 cut(s) 880
BsrI ACTGG 5 cut(s) 208, 233, 299, 388, 709
BssECI CCNNGG 1 cut(s) 1329
BssMI GATC 6 cut(s) 256, 300, 418, 728, 819, 955
Bst2UI CCWGG 2 cut(s) 516, 1331
Bst4CI ACNGT 1 cut(s) 747
Bst6I CTCTTC 1 cut(s) 1116
BstDEI CTNAG 4 cut(s) 139, 580, 794, 1300
BstF5I GGATG 6 cut(s) 268, 886, 1027, 1132, 1213, 1249
BstKTI GATC 6 cut(s) 259, 303, 421, 731, 822, 958
BstMAI GTCTC 2 cut(s) 583, 1292
BstMBI GATC 6 cut(s) 256, 300, 418, 728, 819, 955
BstMWI GCNNNNNNNGC 3 cut(s) 54, 500, 549
BstNI CCWGG 2 cut(s) 516, 1331
BstSCI CCNGG 2 cut(s) 514, 1329
BstSFI CTRYAG 1 cut(s) 144
BstV1I GCAGC 2 cut(s) 57, 130
BstV2I GAAGAC 1 cut(s) 106
BstX2I RGATCY 2 cut(s) 728, 955
BstXI CCANNNNNNTGG 1 cut(s) 320
BstYI RGATCY 2 cut(s) 728, 955
BsuI GTATCC 1 cut(s) 1148
BsuRI GGCC 2 cut(s) 849, 1108
BtsCI GGATG 6 cut(s) 268, 886, 1027, 1132, 1213, 1249
BtsIMutI CAGTG 2 cut(s) 201, 226
Cfr13I GGNCC 1 cut(s) 1106
CseI GACGC 1 cut(s) 434
Csp6I GTAC 7 cut(s) 234, 310, 400, 436, 648, 1196, 1219
CviAII CATG 8 cut(s) 95, 464, 620, 631, 688, 868, 1067, 1084
CviQI GTAC 7 cut(s) 234, 310, 400, 436, 648, 1196, 1219
DdeI CTNAG 4 cut(s) 139, 580, 794, 1300
DpnI GATC 6 cut(s) 258, 302, 420, 730, 821, 957
DpnII GATC 6 cut(s) 256, 300, 418, 728, 819, 955
DraI TTTAAA 1 cut(s) 247
DraIII CACNNNGTG 1 cut(s) 1069
DriI GACNNNNNGTC 1 cut(s) 110
EaeI YGGCCR 1 cut(s) 847
Eam1104I CTCTTC 1 cut(s) 1116
Eam1105I GACNNNNNGTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 1116
EciI GGCGGA 1 cut(s) 558
Eco24I GRGCYC 1 cut(s) 770
Eco31I GGTCTC 1 cut(s) 1292
Eco57I CTGAAG 1 cut(s) 516
EcoO109I RGGNCCY 1 cut(s) 1106
EcoRII CCWGG 2 cut(s) 514, 1329
EcoT38I GRGCYC 1 cut(s) 770
FaeI CATG 8 cut(s) 98, 467, 623, 634, 691, 871, 1070, 1087
FaqI GGGAC 2 cut(s) 228, 437
FatI CATG 8 cut(s) 94, 463, 619, 630, 687, 867, 1066, 1083
FbaI TGATCA 1 cut(s) 819
Fnu4HI GCNGC 2 cut(s) 46, 144
FokI GGATG 6 cut(s) 275, 873, 1034, 1119, 1220, 1256
FriOI GRGCYC 1 cut(s) 770
Fsp4HI GCNGC 2 cut(s) 46, 144
FspBI CTAG 3 cut(s) 1257, 1269, 1317
GluI GCNGC 2 cut(s) 46, 144
GsaI CCCAGC 1 cut(s) 1059
GsuI CTGGAG 2 cut(s) 741, 1200
HaeIII GGCC 2 cut(s) 849, 1108
HgaI GACGC 1 cut(s) 434
Hin1II CATG 8 cut(s) 98, 467, 623, 634, 691, 871, 1070, 1087
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 1 cut(s) 1142
HphI GGTGA 4 cut(s) 266, 398, 764, 1055
Hpy166II GTNNAC 3 cut(s) 6, 381, 527
Hpy188I TCNGA 4 cut(s) 583, 797, 1292, 1350
Hpy188III TCNNGA 8 cut(s) 62, 260, 283, 422, 614, 720, 773, 890
Hpy8I GTNNAC 3 cut(s) 6, 381, 527
HpyAV CCTTC 5 cut(s) 287, 517, 942, 1145, 1234
HpyCH4III ACNGT 1 cut(s) 747
HpyCH4V TGCA 8 cut(s) 146, 271, 405, 503, 674, 737, 811, 979
HpyF10VI GCNNNNNNNGC 3 cut(s) 54, 500, 549
HpyF3I CTNAG 4 cut(s) 139, 580, 794, 1300
Hsp92II CATG 8 cut(s) 98, 467, 623, 634, 691, 871, 1070, 1087
KpnI GGTACC 1 cut(s) 237
Ksp22I TGATCA 1 cut(s) 819
Kzo9I GATC 6 cut(s) 256, 300, 418, 728, 819, 955
LmnI GCTCC 1 cut(s) 627
Lsp1109I GCAGC 2 cut(s) 57, 130
LweI GCATC 4 cut(s) 490, 672, 895, 988
MaeI CTAG 3 cut(s) 1257, 1269, 1317
MaeIII GTNAC 1 cut(s) 386
MalI GATC 6 cut(s) 258, 302, 420, 730, 821, 957
MboI GATC 6 cut(s) 256, 300, 418, 728, 819, 955
MboII GAAGA 5 cut(s) 106, 509, 950, 1103, 1184
MfeI CAATTG 1 cut(s) 840
MflI RGATCY 2 cut(s) 728, 955
MhlI GDGCHC 1 cut(s) 770
MmeI TCCRAC 4 cut(s) 139, 999, 1160, 1328
MroXI GAANNNNTTC 1 cut(s) 459
MseI TTAA 6 cut(s) 246, 653, 827, 1125, 1343, 1355
MslI CAYNNNNRTG 1 cut(s) 1071
MspA1I CMGCKG 1 cut(s) 143
MspR9I CCNGG 2 cut(s) 516, 1331
MunI CAATTG 1 cut(s) 840
Mva1269I GAATGC 1 cut(s) 1059
MvaI CCWGG 2 cut(s) 516, 1331
MwoI GCNNNNNNNGC 3 cut(s) 54, 500, 549
NdeII GATC 6 cut(s) 256, 300, 418, 728, 819, 955
NlaIII CATG 8 cut(s) 98, 467, 623, 634, 691, 871, 1070, 1087
NlaIV GGNNCC 1 cut(s) 235
NmuCI GTSAC 1 cut(s) 386
PctI GAATGC 1 cut(s) 1059
PdmI GAANNNNTTC 1 cut(s) 459
PfeI GAWTC 1 cut(s) 1142
PkrI GCNGC 2 cut(s) 47, 145
PshBI ATTAAT 2 cut(s) 653, 827
Psp6I CCWGG 2 cut(s) 514, 1329
PspFI CCCAGC 1 cut(s) 1055
PspGI CCWGG 2 cut(s) 514, 1329
PspN4I GGNNCC 1 cut(s) 235
PspPI GGNCC 1 cut(s) 1106
PstI CTGCAG 1 cut(s) 148
PsuI RGATCY 2 cut(s) 728, 955
PvuII CAGCTG 1 cut(s) 143
RsaI GTAC 7 cut(s) 235, 311, 401, 437, 649, 1197, 1220
RsaNI GTAC 7 cut(s) 234, 310, 400, 436, 648, 1196, 1219
RseI CAYNNNNRTG 1 cut(s) 1071
SaqAI TTAA 6 cut(s) 246, 653, 827, 1125, 1343, 1355
SatI GCNGC 2 cut(s) 46, 144
Sau3AI GATC 6 cut(s) 256, 300, 418, 728, 819, 955
Sau96I GGNCC 1 cut(s) 1106
ScaI AGTACT 1 cut(s) 401
ScrFI CCNGG 2 cut(s) 516, 1331
SduI GDGCHC 1 cut(s) 770
SfaNI GCATC 4 cut(s) 490, 672, 895, 988
SfcI CTRYAG 1 cut(s) 144
SmiMI CAYNNNNRTG 1 cut(s) 1071
SmlI CTYRAG 2 cut(s) 537, 602
SmoI CTYRAG 2 cut(s) 537, 602
SsiI CCGC 2 cut(s) 76, 543
SspI AATATT 1 cut(s) 1253
SspMI CTAG 3 cut(s) 1257, 1269, 1317
StyD4I CCNGG 2 cut(s) 514, 1329
TaaI ACNGT 1 cut(s) 747
TaqI TCGA 2 cut(s) 284, 999
TatI WGTACW 3 cut(s) 399, 647, 1195
TfiI GAWTC 1 cut(s) 1142
Tru1I TTAA 6 cut(s) 246, 653, 827, 1125, 1343, 1355
Tru9I TTAA 6 cut(s) 246, 653, 827, 1125, 1343, 1355
TscAI CASTG 2 cut(s) 208, 233
TseFI GTSAC 1 cut(s) 386
TseI GCWGC 2 cut(s) 45, 143
Tsp45I GTSAC 1 cut(s) 386
TspGWI ACGGA 3 cut(s) 320, 1220, 1236
TspRI CASTG 2 cut(s) 208, 233
VspI ATTAAT 2 cut(s) 653, 827
XapI RAATTY 2 cut(s) 286, 878
XcmI CCANNNNNNNNNTGG 3 cut(s) 621, 716, 1280
XmnI GAANNNNTTC 1 cut(s) 459
XspI CTAG 3 cut(s) 1257, 1269, 1317
ZrmI AGTACT 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.