Rorug05G0188500

1,4-alpha-glucan-branching enzyme 3, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003672
Physical Location & Seq
Reverse (-)
18327324 .. 18327927
604 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0188500.1

Sequence Viewer

Length: 510 bp
ATGAAAAGCCTAGATAATGTTGCCTACAAGTGGATGACAGCACCCGAGAGACCAGCTATACATTGGTGTCAGGCTTTCTTCAACACAGTTGTGGACTGTGACATCATGATTAACAACATCTGTGAGAGTTTCAATGCGTGGATATTAGATGCTAGGGGTAAGCCCCCTGTGACTATGTTGGAAGAATTAAGGGTGAAGCTAATGATACGGATTGCAGTGAGGAAGGAAAAAATGGAAGGGTACCATGGGAATATCTGCCCCAAGCCAAGGGCTATTATAGAGAGAAACAAGGAAAAGGCTGCTGTGGACTGTATTGCAACATTCAATGGTGGAGACATTGCTGAGGTTGACAATATTGAGGGATCAAAGAATGTAGTCAACCTTGCAATGAGGACATGCACTTGCAGGAGGTGGCACTTGACTGGAATCCCCTGCAAGCATGCTATTTCTGCCATATACATGAAGAGGGAAGACCCGGATGATTATGTGGCAGCATGCTATCTCAAGTAG

Protein Analysis

169

Amino Acids

19.28

Weight (kDa)

8.35

Isoelectric Point (pI)

51.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIM PF04434 125 - 155 6.7e-10 SWIM zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 240
AccB1I GGYRCC 1 cut(s) 240
AclWI GGATC 1 cut(s) 370
AfaI GTAC 1 cut(s) 242
AfiI CCNNNNNNNGG 2 cut(s) 30, 267
AgsI TTSAA 3 cut(s) 82, 133, 325
AleI CACNNNNGTG 1 cut(s) 89
AluBI AGCT 2 cut(s) 56, 199
AluI AGCT 2 cut(s) 56, 199
Alw26I GTCTC 2 cut(s) 43, 327
AlwI GGATC 1 cut(s) 370
Ama87I CYCGRG 1 cut(s) 44
ApeKI GCWGC 2 cut(s) 299, 491
Asp718I GGTACC 1 cut(s) 240
AsuC2I CCSGG 1 cut(s) 476
AsuHPI GGTGA 1 cut(s) 205
AvaI CYCGRG 1 cut(s) 44
BanI GGYRCC 1 cut(s) 240
BbsI GAAGAC 1 cut(s) 477
BbvCI CCTCAGC 1 cut(s) 342
BbvI GCAGC 2 cut(s) 286, 503
BcnI CCSGG 1 cut(s) 476
BcoDI GTCTC 2 cut(s) 43, 327
BfaI CTAG 2 cut(s) 11, 153
BisI GCNGC 2 cut(s) 300, 492
BlsI GCNGC 2 cut(s) 301, 493
Bme1390I CCNGG 1 cut(s) 476
BmeT110I CYCGRG 1 cut(s) 44
BmiI GGNNCC 1 cut(s) 242
BmrFI CCNGG 1 cut(s) 476
BmsI GCATC 1 cut(s) 139
BpiI GAAGAC 1 cut(s) 477
Bpu10I CCTNAGC 1 cut(s) 342
BpuEI CTTGAG 1 cut(s) 488
BpuMI CCSGG 1 cut(s) 476
BsaI GGTCTC 1 cut(s) 43
BsaJI CCNNGG 2 cut(s) 244, 266
Bsc4I CCNNNNNNNGG 2 cut(s) 30, 267
Bse1I ACTGG 1 cut(s) 427
Bse3DI GCAATG 2 cut(s) 336, 393
BseDI CCNNGG 2 cut(s) 244, 266
BseGI GGATG 2 cut(s) 39, 484
BseLI CCNNNNNNNGG 2 cut(s) 30, 267
BseMI GCAATG 2 cut(s) 336, 393
BseMII CTCAG 1 cut(s) 333
BseNI ACTGG 1 cut(s) 427
BseXI GCAGC 2 cut(s) 286, 503
BshNI GGYRCC 1 cut(s) 240
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 1 cut(s) 476
BslI CCNNNNNNNGG 2 cut(s) 30, 267
BsmAI GTCTC 2 cut(s) 43, 327
Bso31I GGTCTC 1 cut(s) 43
BsoBI CYCGRG 1 cut(s) 44
Bsp143I GATC 1 cut(s) 362
Bsp19I CCATGG 1 cut(s) 244
BspCNI CTCAG 1 cut(s) 334
BspHI TCATGA 1 cut(s) 105
BspLI GGNNCC 1 cut(s) 242
BspPI GGATC 1 cut(s) 370
BspT107I GGYRCC 1 cut(s) 240
BspTNI GGTCTC 1 cut(s) 43
BsrDI GCAATG 2 cut(s) 336, 393
BsrI ACTGG 1 cut(s) 427
BssECI CCNNGG 2 cut(s) 244, 266
BssMI GATC 1 cut(s) 362
BssT1I CCWWGG 2 cut(s) 244, 266
Bst4CI ACNGT 3 cut(s) 88, 98, 311
Bst6I CTCTTC 1 cut(s) 458
BstC8I GCNNGC 3 cut(s) 437, 441, 496
BstDEI CTNAG 1 cut(s) 342
BstDSI CCRYGG 1 cut(s) 244
BstF5I GGATG 2 cut(s) 39, 484
BstKTI GATC 1 cut(s) 365
BstMAI GTCTC 2 cut(s) 43, 327
BstMBI GATC 1 cut(s) 362
BstMWI GCNNNNNNNGC 1 cut(s) 449
BstNSI RCATGY 3 cut(s) 399, 443, 498
BstSCI CCNGG 1 cut(s) 474
BstV1I GCAGC 2 cut(s) 286, 503
BstV2I GAAGAC 1 cut(s) 477
BtgI CCRYGG 1 cut(s) 244
BtsCI GGATG 2 cut(s) 39, 484
BtsI GCAGTG 1 cut(s) 222
BtsIMutI CAGTG 1 cut(s) 222
Cac8I GCNNGC 3 cut(s) 437, 441, 496
CciI TCATGA 1 cut(s) 105
Csp6I GTAC 1 cut(s) 241
CviAII CATG 6 cut(s) 106, 245, 396, 440, 460, 495
CviJI RGCY 8 cut(s) 9, 56, 74, 163, 199, 265, 272, 299
CviKI_1 RGCY 8 cut(s) 9, 56, 74, 163, 199, 265, 272, 299
CviQI GTAC 1 cut(s) 241
DdeI CTNAG 1 cut(s) 342
DpnI GATC 1 cut(s) 364
DpnII GATC 1 cut(s) 362
Eam1104I CTCTTC 1 cut(s) 458
EarI CTCTTC 1 cut(s) 458
Eco130I CCWWGG 2 cut(s) 244, 266
Eco31I GGTCTC 1 cut(s) 43
Eco88I CYCGRG 1 cut(s) 44
EcoT14I CCWWGG 2 cut(s) 244, 266
ErhI CCWWGG 2 cut(s) 244, 266
FaeI CATG 6 cut(s) 109, 248, 399, 443, 463, 498
FatI CATG 6 cut(s) 105, 244, 395, 439, 459, 494
Fnu4HI GCNGC 2 cut(s) 300, 492
FokI GGATG 2 cut(s) 46, 491
Fsp4HI GCNGC 2 cut(s) 300, 492
FspBI CTAG 2 cut(s) 11, 153
GluI GCNGC 2 cut(s) 300, 492
HapII CCGG 1 cut(s) 476
Hin1II CATG 6 cut(s) 109, 248, 399, 443, 463, 498
HincII GTYRAC 2 cut(s) 349, 379
HindII GTYRAC 2 cut(s) 349, 379
HinfI GANTC 1 cut(s) 426
HpaII CCGG 1 cut(s) 476
HphI GGTGA 1 cut(s) 205
Hpy166II GTNNAC 4 cut(s) 94, 307, 349, 379
Hpy188III TCNNGA 1 cut(s) 106
Hpy8I GTNNAC 4 cut(s) 94, 307, 349, 379
HpyAV CCTTC 2 cut(s) 217, 230
HpyCH4III ACNGT 3 cut(s) 88, 98, 311
HpyCH4V TGCA 6 cut(s) 215, 317, 386, 399, 405, 435
HpyF10VI GCNNNNNNNGC 1 cut(s) 449
HpyF3I CTNAG 1 cut(s) 342
Hsp92II CATG 6 cut(s) 109, 248, 399, 443, 463, 498
KpnI GGTACC 1 cut(s) 244
Kzo9I GATC 1 cut(s) 362
LpnPI CCDG 7 cut(s) 56, 66, 180, 391, 408, 445, 489
Lsp1109I GCAGC 2 cut(s) 286, 503
LweI GCATC 1 cut(s) 139
MaeI CTAG 2 cut(s) 11, 153
MaeIII GTNAC 2 cut(s) 98, 169
MalI GATC 1 cut(s) 364
MboI GATC 1 cut(s) 362
MboII GAAGA 4 cut(s) 70, 194, 475, 482
MluCI AATT 1 cut(s) 185
MmeI TCCRAC 1 cut(s) 159
MnlI CCTC 6 cut(s) 213, 337, 352, 384, 402, 459
MseI TTAA 2 cut(s) 111, 188
MslI CAYNNNNRTG 2 cut(s) 89, 458
MspI CCGG 1 cut(s) 476
MspR9I CCNGG 1 cut(s) 476
MwoI GCNNNNNNNGC 1 cut(s) 449
NciI CCSGG 1 cut(s) 476
NcoI CCATGG 1 cut(s) 244
NdeII GATC 1 cut(s) 362
NlaIII CATG 6 cut(s) 109, 248, 399, 443, 463, 498
NlaIV GGNNCC 1 cut(s) 242
NmuCI GTSAC 2 cut(s) 98, 169
NspI RCATGY 3 cut(s) 399, 443, 498
OliI CACNNNNGTG 1 cut(s) 89
PaeI GCATGC 2 cut(s) 443, 498
PagI TCATGA 1 cut(s) 105
PfeI GAWTC 1 cut(s) 426
PkrI GCNGC 2 cut(s) 301, 493
PspN4I GGNNCC 1 cut(s) 242
RsaI GTAC 1 cut(s) 242
RsaNI GTAC 1 cut(s) 241
RseI CAYNNNNRTG 2 cut(s) 89, 458
SaqAI TTAA 2 cut(s) 111, 188
SatI GCNGC 2 cut(s) 300, 492
Sau3AI GATC 1 cut(s) 362
ScrFI CCNGG 1 cut(s) 476
SetI ASST 5 cut(s) 58, 201, 348, 384, 413
SfaNI GCATC 1 cut(s) 139
SmiMI CAYNNNNRTG 2 cut(s) 89, 458
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
SphI GCATGC 2 cut(s) 443, 498
Sse9I AATT 1 cut(s) 185
SspI AATATT 1 cut(s) 355
SspMI CTAG 2 cut(s) 11, 153
StyD4I CCNGG 1 cut(s) 474
StyI CCWWGG 2 cut(s) 244, 266
TaaI ACNGT 3 cut(s) 88, 98, 311
TasI AATT 1 cut(s) 185
TfiI GAWTC 1 cut(s) 426
Tru1I TTAA 2 cut(s) 111, 188
Tru9I TTAA 2 cut(s) 111, 188
TscAI CASTG 1 cut(s) 222
TseFI GTSAC 2 cut(s) 98, 169
TseI GCWGC 2 cut(s) 299, 491
Tsp45I GTSAC 2 cut(s) 98, 169
TspDTI ATGAA 2 cut(s) 17, 476
TspGWI ACGGA 1 cut(s) 223
TspRI CASTG 1 cut(s) 222
XceI RCATGY 3 cut(s) 399, 443, 498
XcmI CCANNNNNNNNNTGG 1 cut(s) 60
XspI CTAG 2 cut(s) 11, 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.