Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0003672

Physical Location & Seq

Genomic Coordinates Forward (+)

35705070 .. 35715042

Gene Structure 9973 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG287300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2583 bp

ATGGCTTCACTTTCACTTTCAACCAAGTTCCCTCTGTACCCCAACCTCACATTTCTCCAATTCCAGTACCCAAACAGACCTCAAAGAATCATCTTTCCTATCAAGAAGACAACCCAGATAGGCTGCTCCGCCACAGGTCAACCCCACCAACAACAAAGACCACCCAAGAACAAGAAGAAAATCCTCACCGACGGCGACAAAGGCGTTGACCCAGTTGGCTTCCTCTCCAAATTCGACATCTCCCATAAGCAATTTGCTCAGTTCCTCCGTGAAAGACACAAAGCCCTCAAGGACCTGACAGATGAAATATTAACTCGCCACATCAACCTCAGGGATATGTCTTCTGGATATGAGATATTGGGTTTGCATCGACACCCAGAACATAGAGAGGATTATATGGAGTGGGCTCCAGGTGCTCGCTACTGCGCACTGGTTGGTGATTTCAATGGGTGGTCTCCTACAGAGAACTGTGCAAAAGAGGGTCATTTTGGACATGATGACTATGGATACTGGTTCATCATTCTTGAAGATAAGTTGAGGAATGGAGAGAAACCAGATGAGCTGTACTTTCAACAGTATAACTATGTAGATGATGACGATAAAGGTGACAGTGGTGTTACTATTGAAGAAGTCTTTAAGAAAGCAAATGATGAGTATTGGGAACCTGGAGAAGACCGGTTTCTAAAAAACCGCTTAGAAGTACCAGCAAAGTTGTATGAACAGATTTTTGGGCCTAATGGACCTCAAACATTAGAGGAACTTGAAGAAATACCAGATGCAGAAACAAGATATAAAGCGTGGAAAGAACAGCATAAAGATGACCCGCCTAGTAACTTACCTTCTTATGATGTGATTGACAATGGAAAAGAGTATGACATTTACAATGTTGTACTTGATCCAGTATCACAAGAGAAATTTAAAGCAAAGAAGCCTCCCTTGCCATATTGGTTCGAAACACGTAAAGGAAGGAAGGCATGGTTGAAAAAGTATAGGCCGTGCATTCCTCATGGGAGCAAGTACAGGGTCTATTTTAATACTCCTAGTGGGCCACTGGAACGAGTACCTGCTTGGGCAACTTTTGTGGAACCAGACGCAGATGGAGGACAAGCTTTTGCAATCCACTGGGAACCACCCCCAGAACTTGCATACAAATGGAAGAATGCACGCCCTAAAGTGCCAAAGTCCCTGCGCATTTATGAATGTCATGTTGGCATAAGTGGATCAGAGCCTAAAGTATCTTCTTTCAGTGATTTCACAGAGAAGGTTCTTCCTCATGTAAAGGAGGCTGGCTATAATGCAATTCAGCTGATTGGAGTTATTGAGCACAAAGATTATTTCACTGTTGGTTATAGAGCCACTAATTTATATGCTGTTAGCAGCCGATTTGGCACTCCTGATGATTTCAAGCGGTTGGTTGATGAAGCACATGGACAAGGACTGCTGGTCTTCTTGGAGATTGTCCATTCCTACTCAGCTGCAGATGAGATGGTTGGATTAGGACTCTTTGATGGAACAAATGACTGCTACTTCCATACTGGCAAACGGGGACATCACAAGCACTGGGGTACCAGAATGTTTAAATATGGTGATCTGGATGTTCTGCATTTCCTTCTCTCGAATTTGAACTGGTGGATCACGGAGTACCAAGTTGATGGTTTTCAGTTCCATTCACTCTCATCAATGATTTATACTCATAATGGTTTTGCTTCGTTTACCGGTGACTTGGAGGAGTACTGCAATCAATATGTTGATAGGGACGCATTGTCATACCTCATTTTGGCAAACGAAATACTTCATGCTCTCCACCCAGATATAATAACGATTGCTGAAGATGCAACCTTCTATCCAGGTCTATGTGAACCAATCTCTCAAGGCGGATTGGGCTTTGATTATTGTGTCAATCTTTCTGTCTCAGAGATGTGGTCATCTTTCCTTGAGAATGTTCCAGACCATGATTGGAGCATGAGTAAGATTGTGAGTACATTAATGGGCAACAAAAATTATGCAGATAAGATGCTCATGTATGCTGAAAATCATAACCAGTCAATTTCTGGAGGGAGATCTTTTGCCGAGATACTGTTTGGTGAAATCAGGGAGGGCTCTCCTGACAAGGAGAAATTATTGCTCAGAGGTTGTTCCTTGCACAAGATGATCAGATTAATTACCATGACAATTGGTGGCCGTGCTTATCTCAACTTCATGGGCAATGAATTTGGGCATCCAGAGAGGGTTGAGTTCCCAATGCCAAGCAACAATTTCTCATTTTCTCTTGCCAACCGAAGGTGGGATCTTCTTGCCAATGAGGGATTGCATCTCAACTTGTTTACCTTCGATAAGGACTTGATGAAGTTGGATGAAAATGAAAGAATACTTCCAAGAGTTTTACCCAGCATTCACCATGTGAATGACAATAGCATGGTAATTGCTTACATAAGAGGGCCTCTTCTATTCGTATTTAACTTTCATCCAACAGATTCTTATGAAGGATACCGAATAGGTGTAGAAGAAGCTGGAGAGTATCAAGTAGGTTATGGCAAACAGCATTTAGTACTTTTATGTCAGAAATATAGTTCCAAATCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0007275 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009791 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0048856 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko01110 map00500 map01100 map01110

Pfam Domains

Protein Families

Alpha-amylase Alpha-amylase_C CBM_48

Protein Analysis

860

Amino Acids

99.0

Weight (kDa)

5.86

Isoelectric Point (pI)

34.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Alpha-amylase	PF00128	423 - 491	1e-06	Alpha amylase, catalytic domain
Alpha-amylase_C	PF02806	800 - 842	2.2e-10	Alpha amylase, C-terminal all-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003672)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20440 AT3G20440 AT3G20440
fragaria_vesca	FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220 FvH4_3g23220
malus_domestica	MD11G1212700.v1.1
prunus_persica	Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1 Prupe.4G219000_v2.0.a1
pyrus_communis	pycom11g18770
rosa_chinensis	RchiOBHm_Chr5g0040901 RchiOBHm_Chr5g0040911
rosa_laevigata	RLG00000034012
rosa_multiflora	Rmu_sc0002848.1_g000016 Rmu_ssc0000416.1_g000003
rosa_roxburghii	Rroxscaffold_1G00039790
rosa_rugosa	Rorug05G0188500 Rorug05G0188600 Rorug05G0188600
rosa_samantha	Rh5AG274100 Rh5BG279100 Rh5CG311200 Rh5DG287300
rosa_wichuraiana	Rw5G025750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	427, 1190
Acc36I	ACCTGC	1 cut(s)	1072
Acc65I	GGTACC	1 cut(s)	1565
AccB1I	GGYRCC	1 cut(s)	1565
AciI	CCGC	5 cut(s)	129, 691, 824, 1408, 1875
AclWI	GGATC	4 cut(s)	890, 1228, 1640, 2295
AcoI	YGGCCR	1 cut(s)	2179
AcsI	RAATTY	4 cut(s)	230, 914, 1618, 2210
AcuI	CTGAAG	1 cut(s)	1848
AdeI	CACNNNGTG	1 cut(s)	2401
AfiI	CCNNNNNNNGG	3 cut(s)	1777, 2280, 2284
AflIII	ACRYGT	1 cut(s)	956
AgeI	ACCGGT	2 cut(s)	675, 1715
AgsI	TTSAA	9 cut(s)	21, 445, 527, 572, 626, 764, 982, 1405, 1624
AhdI	GACNNNNNGTC	2 cut(s)	1442, 1762
AjnI	CCWGG	3 cut(s)	409, 664, 1846
AjuI	GAANNNNNNNTTGG	4 cut(s)	711, 743, 1191, 1223
AluBI	AGCT	5 cut(s)	562, 1109, 1306, 1475, 2510
AluI	AGCT	5 cut(s)	562, 1109, 1306, 1475, 2510
Alw21I	GWGCWC	2 cut(s)	418, 1326
Alw26I	GTCTC	2 cut(s)	459, 1915
AlwI	GGATC	4 cut(s)	890, 1228, 1640, 2295
AoxI	GGCC	5 cut(s)	731, 992, 1046, 2179, 2438
ApeKI	GCWGC	3 cut(s)	123, 1377, 1475
ApoI	RAATTY	4 cut(s)	230, 914, 1618, 2210
AseI	ATTAAT	2 cut(s)	1985, 2159
AsiGI	ACCGGT	2 cut(s)	675, 1715
Asp700I	GAANNNNTTC	1 cut(s)	1791
Asp718I	GGTACC	1 cut(s)	1565
AspLEI	GCGC	2 cut(s)	428, 1191
AspS9I	GGNCC	5 cut(s)	292, 731, 740, 1046, 2438
AsuHPI	GGTGA	7 cut(s)	178, 449, 617, 1598, 1730, 2096, 2387
AsuII	TTCGAA	1 cut(s)	951
AvaII	GGWCC	2 cut(s)	292, 740
AxyI	CCTNAGG	1 cut(s)	329
BaeI	ACNNNNGTAYC	2 cut(s)	1549, 1582
BanI	GGYRCC	1 cut(s)	1565
BanII	GRGCYC	2 cut(s)	409, 2102
BarI	GAAGNNNNNNTAC	2 cut(s)	823, 855
BbsI	GAAGAC	4 cut(s)	113, 333, 678, 1438
Bbv12I	GWGCWC	2 cut(s)	418, 1326
BbvI	GCAGC	3 cut(s)	110, 1389, 1462
BccI	CCATC	4 cut(s)	1091, 1480, 1502, 1646
BceAI	ACGGC	3 cut(s)	208, 979, 2166
BciT130I	CCWGG	3 cut(s)	411, 666, 1848
BciVI	GTATCC	2 cut(s)	500, 2480
BclI	TGATCA	1 cut(s)	2151
BcoDI	GTCTC	2 cut(s)	459, 1915
BfaI	CTAG	2 cut(s)	828, 1041
BfmI	CTRYAG	2 cut(s)	459, 1476
BfuAI	ACCTGC	1 cut(s)	1072
BfuI	GTATCC	2 cut(s)	500, 2480
BglI	GCCNNNNNGGC	1 cut(s)	1386
BglII	AGATCT	1 cut(s)	2060
BisI	GCNGC	3 cut(s)	124, 1378, 1476
BlsI	GCNGC	3 cut(s)	125, 1379, 1477
BmcAI	AGTACT	2 cut(s)	1733, 2550
Bme1390I	CCNGG	3 cut(s)	411, 666, 1848
Bme18I	GGWCC	2 cut(s)	292, 740
BmeRI	GACNNNNNGTC	2 cut(s)	1442, 1762
BmgT120I	GGNCC	5 cut(s)	292, 731, 740, 1046, 2438
BmiI	GGNNCC	5 cut(s)	408, 663, 1086, 1128, 1567
BmrFI	CCNGG	3 cut(s)	411, 666, 1848
BmrI	ACTGGG	3 cut(s)	206, 1132, 1570
BmsI	GCATC	6 cut(s)	376, 766, 1822, 2004, 2227, 2320
BmuI	ACTGGG	3 cut(s)	206, 1132, 1570
BpiI	GAAGAC	4 cut(s)	113, 333, 678, 1438
BpmI	CTGGAG	4 cut(s)	393, 687, 2073, 2532
Bpu14I	TTCGAA	1 cut(s)	951
BpuEI	CTTGAG	3 cut(s)	272, 1854, 1955
BsaAI	YACGTR	1 cut(s)	959
BsaI	GGTCTC	1 cut(s)	459
BsaWI	WCCGGW	2 cut(s)	675, 1715
BsaXI	ACNNNNNCTCC	2 cut(s)	1951, 1981
Bsc4I	CCNNNNNNNGG	3 cut(s)	1777, 2280, 2284
Bse118I	RCCGGY	2 cut(s)	675, 1715
Bse21I	CCTNAGG	1 cut(s)	329
Bse3DI	GCAATG	1 cut(s)	2212
BseBI	CCWGG	3 cut(s)	411, 666, 1848
BseGI	GGATG	4 cut(s)	1600, 2218, 2359, 2464
BseLI	CCNNNNNNNGG	3 cut(s)	1777, 2280, 2284
BseMI	GCAATG	1 cut(s)	2212
BseMII	CTCAG	5 cut(s)	272, 343, 1485, 1926, 2140
BseRI	GAGGAG	1 cut(s)	1742
BseXI	GCAGC	3 cut(s)	110, 1389, 1462
BseYI	CCCAGC	1 cut(s)	2387
BshFI	GGCC	5 cut(s)	733, 994, 1048, 2181, 2440
BshNI	GGYRCC	1 cut(s)	1565
BshTI	ACCGGT	2 cut(s)	675, 1715
BsiHKAI	GWGCWC	2 cut(s)	418, 1326
BsiSI	CCGG	2 cut(s)	676, 1716
BslFI	GGGAC	3 cut(s)	1168, 1560, 1769
BslI	CCNNNNNNNGG	3 cut(s)	1777, 2280, 2284
BsmAI	GTCTC	2 cut(s)	459, 1915
BsmFI	GGGAC	3 cut(s)	1168, 1560, 1769
BsmI	GAATGC	3 cut(s)	999, 1165, 2391
BsnI	GGCC	5 cut(s)	733, 994, 1048, 2181, 2440
Bso31I	GGTCTC	1 cut(s)	459
Bsp119I	TTCGAA	1 cut(s)	951
Bsp1286I	GDGCHC	4 cut(s)	409, 418, 1326, 2102
Bsp143I	GATC	7 cut(s)	895, 1220, 1588, 1632, 2060, 2151, 2287
BspACI	CCGC	5 cut(s)	129, 691, 824, 1408, 1875
BspANI	GGCC	5 cut(s)	733, 994, 1048, 2181, 2440
BspCNI	CTCAG	5 cut(s)	271, 342, 1484, 1925, 2139
BspLI	GGNNCC	5 cut(s)	408, 663, 1086, 1128, 1567
BspMAI	CTGCAG	1 cut(s)	1480
BspMI	ACCTGC	1 cut(s)	1072
BspPI	GGATC	4 cut(s)	890, 1228, 1640, 2295
BspT104I	TTCGAA	1 cut(s)	951
BspT107I	GGYRCC	1 cut(s)	1565
BspTNI	GGTCTC	1 cut(s)	459
BsrDI	GCAATG	1 cut(s)	2212
BsrFI	RCCGGY	2 cut(s)	675, 1715
BssAI	RCCGGY	2 cut(s)	675, 1715
BssMI	GATC	7 cut(s)	895, 1220, 1588, 1632, 2060, 2151, 2287
Bst2UI	CCWGG	3 cut(s)	411, 666, 1848
Bst4CI	ACNGT	5 cut(s)	470, 576, 611, 1342, 2079
Bst6I	CTCTTC	1 cut(s)	2448
BstBAI	YACGTR	1 cut(s)	959
BstBI	TTCGAA	1 cut(s)	951
BstC8I	GCNNGC	3 cut(s)	418, 1165, 1288
BstDEI	CTNAG	6 cut(s)	258, 329, 694, 1471, 1912, 2126
BstF5I	GGATG	4 cut(s)	1600, 2218, 2359, 2464
BstHHI	GCGC	2 cut(s)	428, 1191
BstKTI	GATC	7 cut(s)	898, 1223, 1591, 1635, 2063, 2154, 2290
BstMAI	GTCTC	2 cut(s)	459, 1915
BstMBI	GATC	7 cut(s)	895, 1220, 1588, 1632, 2060, 2151, 2287
BstMWI	GCNNNNNNNGC	6 cut(s)	201, 413, 937, 1386, 1832, 1881
BstNI	CCWGG	3 cut(s)	411, 666, 1848
BstSCI	CCNGG	3 cut(s)	409, 664, 1846
BstSFI	CTRYAG	2 cut(s)	459, 1476
BstV1I	GCAGC	3 cut(s)	110, 1389, 1462
BstV2I	GAAGAC	4 cut(s)	113, 333, 678, 1438
BstX2I	RGATCY	2 cut(s)	2060, 2287
BstXI	CCANNNNNNTGG	1 cut(s)	1652
BstYI	RGATCY	2 cut(s)	2060, 2287
Bsu36I	CCTNAGG	1 cut(s)	329
BsuI	GTATCC	2 cut(s)	500, 2480
BsuRI	GGCC	5 cut(s)	733, 994, 1048, 2181, 2440
BtsCI	GGATG	4 cut(s)	1600, 2218, 2359, 2464
BtsIMutI	CAGTG	7 cut(s)	428, 616, 1049, 1120, 1252, 1338, 1558
BveI	ACCTGC	1 cut(s)	1072
Cac8I	GCNNGC	3 cut(s)	418, 1165, 1288
CfoI	GCGC	2 cut(s)	428, 1191
Cfr10I	RCCGGY	2 cut(s)	675, 1715
Cfr13I	GGNCC	5 cut(s)	292, 731, 740, 1046, 2438
CseI	GACGC	2 cut(s)	1100, 1766
CspAI	ACCGGT	2 cut(s)	675, 1715
CspCI	CAANNNNNGTGG	2 cut(s)	1062, 1097
DdeI	CTNAG	6 cut(s)	258, 329, 694, 1471, 1912, 2126
DpnI	GATC	7 cut(s)	897, 1222, 1590, 1634, 2062, 2153, 2289
DpnII	GATC	7 cut(s)	895, 1220, 1588, 1632, 2060, 2151, 2287
DraI	TTTAAA	2 cut(s)	919, 1579
DraIII	CACNNNGTG	1 cut(s)	2401
DriI	GACNNNNNGTC	2 cut(s)	1442, 1762
EaeI	YGGCCR	1 cut(s)	2179
Eam1104I	CTCTTC	1 cut(s)	2448
Eam1105I	GACNNNNNGTC	2 cut(s)	1442, 1762
EarI	CTCTTC	1 cut(s)	2448
EciI	GGCGGA	2 cut(s)	118, 1890
Eco24I	GRGCYC	2 cut(s)	409, 2102
Eco31I	GGTCTC	1 cut(s)	459
Eco47I	GGWCC	2 cut(s)	292, 740
Eco57I	CTGAAG	1 cut(s)	1848
Eco81I	CCTNAGG	1 cut(s)	329
EcoO109I	RGGNCCY	2 cut(s)	292, 2438
EcoRII	CCWGG	3 cut(s)	409, 664, 1846
EcoT38I	GRGCYC	2 cut(s)	409, 2102
FalI	AAGNNNNNCTT	2 cut(s)	920, 952
FaqI	GGGAC	3 cut(s)	1168, 1560, 1769
FauI	CCCGC	1 cut(s)	831
FbaI	TGATCA	1 cut(s)	2151
Fnu4HI	GCNGC	3 cut(s)	124, 1378, 1476
FokI	GGATG	4 cut(s)	1607, 2205, 2366, 2451
FriOI	GRGCYC	2 cut(s)	409, 2102
Fsp4HI	GCNGC	3 cut(s)	124, 1378, 1476
FspBI	CTAG	2 cut(s)	828, 1041
FspI	TGCGCA	2 cut(s)	427, 1190
GlaI	GCGC	2 cut(s)	427, 1190
GluI	GCNGC	3 cut(s)	124, 1378, 1476
GsaI	CCCAGC	1 cut(s)	2391
GsuI	CTGGAG	4 cut(s)	393, 687, 2073, 2532
HaeIII	GGCC	5 cut(s)	733, 994, 1048, 2181, 2440
HapII	CCGG	2 cut(s)	676, 1716
HgaI	GACGC	2 cut(s)	1100, 1766
HhaI	GCGC	2 cut(s)	428, 1191
Hin6I	GCGC	2 cut(s)	426, 1189
HinP1I	GCGC	2 cut(s)	426, 1189
HincII	GTYRAC	2 cut(s)	140, 208
HindII	GTYRAC	2 cut(s)	140, 208
HindIII	AAGCTT	1 cut(s)	1107
HinfI	GANTC	3 cut(s)	87, 1500, 2474
HpaII	CCGG	2 cut(s)	676, 1716
HphI	GGTGA	7 cut(s)	178, 449, 617, 1598, 1730, 2096, 2387
Hpy166II	GTNNAC	5 cut(s)	140, 208, 1713, 1859, 2325
Hpy188I	TCNGA	5 cut(s)	1225, 1915, 2129, 2156, 2562
Hpy8I	GTNNAC	5 cut(s)	140, 208, 1713, 1859, 2325
Hpy99I	CGWCG	1 cut(s)	194
HpyAV	CCTTC	9 cut(s)	849, 960, 964, 1255, 1619, 1849, 2274, 2338, 2477
HpyCH4III	ACNGT	5 cut(s)	470, 576, 611, 1342, 2079
HpyCH4IV	ACGT	1 cut(s)	958
HpyF10VI	GCNNNNNNNGC	6 cut(s)	201, 413, 937, 1386, 1832, 1881
HpyF3I	CTNAG	6 cut(s)	258, 329, 694, 1471, 1912, 2126
HpySE526I	ACGT	1 cut(s)	958
HspAI	GCGC	2 cut(s)	426, 1189
KpnI	GGTACC	1 cut(s)	1569
Ksp22I	TGATCA	1 cut(s)	2151
Kzo9I	GATC	7 cut(s)	895, 1220, 1588, 1632, 2060, 2151, 2287
LmnI	GCTCC	4 cut(s)	131, 412, 1011, 1959
Lsp1109I	GCAGC	3 cut(s)	110, 1389, 1462
LweI	GCATC	6 cut(s)	376, 766, 1822, 2004, 2227, 2320
MaeI	CTAG	2 cut(s)	828, 1041
MaeII	ACGT	1 cut(s)	958
MaeIII	GTNAC	4 cut(s)	605, 616, 830, 1718
MalI	GATC	7 cut(s)	897, 1222, 1590, 1634, 2062, 2153, 2289
MboI	GATC	7 cut(s)	895, 1220, 1588, 1632, 2060, 2151, 2287
MfeI	CAATTG	1 cut(s)	2172
MflI	RGATCY	2 cut(s)	2060, 2287
MhlI	GDGCHC	4 cut(s)	409, 418, 1326, 2102
MlyI	GAGTC	1 cut(s)	1494
MmeI	TCCRAC	3 cut(s)	1471, 2331, 2492
MroXI	GAANNNNTTC	1 cut(s)	1791
MseI	TTAA	9 cut(s)	311, 636, 918, 1032, 1578, 1985, 2159, 2457, 2581
MslI	CAYNNNNRTG	3 cut(s)	816, 1150, 2403
MspA1I	CMGCKG	2 cut(s)	1306, 1475
MspI	CCGG	2 cut(s)	676, 1716
MspR9I	CCNGG	3 cut(s)	411, 666, 1848
MunI	CAATTG	1 cut(s)	2172
Mva1269I	GAATGC	3 cut(s)	999, 1165, 2391
MvaI	CCWGG	3 cut(s)	411, 666, 1848
MwoI	GCNNNNNNNGC	6 cut(s)	201, 413, 937, 1386, 1832, 1881
NdeII	GATC	7 cut(s)	895, 1220, 1588, 1632, 2060, 2151, 2287
NlaIV	GGNNCC	5 cut(s)	408, 663, 1086, 1128, 1567
NmeAIII	GCCGAG	1 cut(s)	2095
NmuCI	GTSAC	2 cut(s)	605, 1718
NsbI	TGCGCA	2 cut(s)	427, 1190
NspV	TTCGAA	1 cut(s)	951
PctI	GAATGC	3 cut(s)	999, 1165, 2391
PdmI	GAANNNNTTC	1 cut(s)	1791
PfeI	GAWTC	2 cut(s)	87, 2474
PinAI	ACCGGT	2 cut(s)	675, 1715
PkrI	GCNGC	3 cut(s)	125, 1379, 1477
PleI	GAGTC	1 cut(s)	1494
PpsI	GAGTC	1 cut(s)	1494
Ppu21I	YACGTR	1 cut(s)	959
PpuMI	RGGWCCY	1 cut(s)	292
PshBI	ATTAAT	2 cut(s)	1985, 2159
Psp5II	RGGWCCY	1 cut(s)	292
Psp6I	CCWGG	3 cut(s)	409, 664, 1846
PspFI	CCCAGC	1 cut(s)	2387
PspGI	CCWGG	3 cut(s)	409, 664, 1846
PspN4I	GGNNCC	5 cut(s)	408, 663, 1086, 1128, 1567
PspPI	GGNCC	5 cut(s)	292, 731, 740, 1046, 2438
PspPPI	RGGWCCY	1 cut(s)	292
PstI	CTGCAG	1 cut(s)	1480
PsuI	RGATCY	2 cut(s)	2060, 2287
PvuII	CAGCTG	2 cut(s)	1306, 1475
RseI	CAYNNNNRTG	3 cut(s)	816, 1150, 2403
SaqAI	TTAA	9 cut(s)	311, 636, 918, 1032, 1578, 1985, 2159, 2457, 2581
SatI	GCNGC	3 cut(s)	124, 1378, 1476
Sau3AI	GATC	7 cut(s)	895, 1220, 1588, 1632, 2060, 2151, 2287
Sau96I	GGNCC	5 cut(s)	292, 731, 740, 1046, 2438
ScaI	AGTACT	2 cut(s)	1733, 2550
SchI	GAGTC	1 cut(s)	1494
ScrFI	CCNGG	3 cut(s)	411, 666, 1848
SduI	GDGCHC	4 cut(s)	409, 418, 1326, 2102
SfaNI	GCATC	6 cut(s)	376, 766, 1822, 2004, 2227, 2320
SfcI	CTRYAG	2 cut(s)	459, 1476
SfuI	TTCGAA	1 cut(s)	951
SinI	GGWCC	2 cut(s)	292, 740
SmiMI	CAYNNNNRTG	3 cut(s)	816, 1150, 2403
SmlI	CTYRAG	3 cut(s)	287, 1869, 1934
SmoI	CTYRAG	3 cut(s)	287, 1869, 1934
SsiI	CCGC	5 cut(s)	129, 691, 824, 1408, 1875
SspI	AATATT	1 cut(s)	309
SspMI	CTAG	2 cut(s)	828, 1041
StyD4I	CCNGG	3 cut(s)	409, 664, 1846
TaaI	ACNGT	5 cut(s)	470, 576, 611, 1342, 2079
TaiI	ACGT	1 cut(s)	961
TaqI	TCGA	5 cut(s)	234, 370, 951, 1616, 2331
TatI	WGTACW	6 cut(s)	564, 889, 1017, 1731, 1979, 2548
TfiI	GAWTC	2 cut(s)	87, 2474
Tru1I	TTAA	9 cut(s)	311, 636, 918, 1032, 1578, 1985, 2159, 2457, 2581
Tru9I	TTAA	9 cut(s)	311, 636, 918, 1032, 1578, 1985, 2159, 2457, 2581
TscAI	CASTG	7 cut(s)	435, 616, 1056, 1127, 1252, 1345, 1565
TseFI	GTSAC	2 cut(s)	605, 1718
TseI	GCWGC	3 cut(s)	123, 1377, 1475
Tsp45I	GTSAC	2 cut(s)	605, 1718
TspGWI	ACGGA	2 cut(s)	257, 1652
TspRI	CASTG	7 cut(s)	435, 616, 1056, 1127, 1252, 1345, 1565
VpaK11BI	GGWCC	2 cut(s)	292, 740
VspI	ATTAAT	2 cut(s)	1985, 2159
XapI	RAATTY	4 cut(s)	230, 914, 1618, 2210
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1953, 2048
XmnI	GAANNNNTTC	1 cut(s)	1791
XspI	CTAG	2 cut(s)	828, 1041
ZrmI	AGTACT	2 cut(s)	1733, 2550

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.