FvH4_3g29610

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004781
Physical Location & Seq
Reverse (-)
22614335 .. 22618774
4440 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g29610.t2

Sequence Viewer

Length: 900 bp
ATGATAACTCAGTTTAGCCACCATCATTGTTTTATCCAAAATAAAAGGGTGTTGCTATTTGAACCGCAAATGACTAAGTGTACCCGGCATTTAAAATTAGACCATATATTTCCTGTTTTACCCTATGTAAATAAACCCAGCTATCTCCCCTTTTATCACCCTTTTTGTTTGCTAGAAGAGGAACCGTCTCAGAATCTCATTTTACTTAAAGCTCTACTCACCAGTTACCGTCTTACATTAAGATCAGCTGTCCTCACTAAACCGAAGCCAAGCACTCTCCTTCCCTTTAGTTTTCCTCATCAATTCATAATTTCATATAGCAGGGTTGTTCAGTCCTTCAGTAGTGAAGTCTGCATTGGGTACCACTGCTTTTTGTCTTGGAAGGTTAACAAGAAAGCAACCATGGATGTTGATTCACAACCAACAATGGAGGAAACCATTTTGGTGGGAGATGATCTAATGACGGGCCCGCCATCACCAATAATTCCACCAGAAATTGCTTCTTATGTGCTTGAAGGTGTTGATTTGTGTGATGGGATATTGAAGAATCTATTTCTATGTCTGCAAATCAATGATATTGAGCCATTTTGTCAGGATGAGCTTGTTATGTATAAGCAGTGTGCTGAAAGAAGAGATCGGGAACTGAGGAAACGACTTCTAGACAGTGAGCAGAAGCTGGGGTTGTCGATGCCTTTAAATGATGCAAAAGAAAGAGCTACCCAGCTTGAAAAAGAGGTCACATCATTAGATAGACGGTTCATTCTTGCTAGCGGACTTGAAGGCATAGAAGGATTTCGCCAGAGATGGAGTTTGCATGGTTGCCTTACAGACACCAAGAAAAGGTTGGAGTCCTTGAAGCAGGGAATGGAGAACAGGAAAGTTGTCGCTGTCAGCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

300

Amino Acids

34.46

Weight (kDa)

8.0

Isoelectric Point (pI)

61.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7803 PF25086 135 - 294 3.4e-92 Domain of unknown function (DUF7803)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 360
AccB1I GGYRCC 1 cut(s) 360
AciI CCGC 3 cut(s) 65, 470, 771
AcuI CTGAAG 1 cut(s) 322
AfaI GTAC 2 cut(s) 82, 362
AfiI CCNNNNNNNGG 1 cut(s) 840
AgsI TTSAA 6 cut(s) 62, 515, 544, 728, 779, 856
AjuI GAANNNNNNNTTGG 2 cut(s) 339, 371
AluBI AGCT 7 cut(s) 141, 212, 248, 601, 676, 716, 724
AluI AGCT 7 cut(s) 141, 212, 248, 601, 676, 716, 724
Alw26I GTCTC 1 cut(s) 192
AlwNI CAGNNNCTG 1 cut(s) 676
AoxI GGCC 1 cut(s) 466
ApaI GGGCCC 1 cut(s) 470
Asp700I GAANNNNTTC 1 cut(s) 792
Asp718I GGTACC 1 cut(s) 360
AspS9I GGNCC 2 cut(s) 466, 467
AsuC2I CCSGG 1 cut(s) 85
AsuHPI GGTGA 3 cut(s) 149, 211, 468
AsuNHI GCTAGC 1 cut(s) 767
BaeGI GKGCMC 1 cut(s) 470
BanI GGYRCC 1 cut(s) 360
BanII GRGCYC 1 cut(s) 470
BccI CCATC 4 cut(s) 30, 481, 527, 798
BcnI CCSGG 1 cut(s) 85
BcoDI GTCTC 1 cut(s) 192
BfaI CTAG 3 cut(s) 173, 659, 768
Bme1390I CCNGG 1 cut(s) 85
BmgT120I GGNCC 2 cut(s) 466, 467
BmiI GGNNCC 3 cut(s) 183, 362, 468
BmrFI CCNGG 1 cut(s) 85
BmsI GCATC 2 cut(s) 678, 691
BmtI GCTAGC 1 cut(s) 771
BpuMI CCSGG 1 cut(s) 85
BsaJI CCNNGG 1 cut(s) 402
Bsc4I CCNNNNNNNGG 1 cut(s) 840
Bse1I ACTGG 1 cut(s) 222
BseDI CCNNGG 1 cut(s) 402
BseGI GGATG 2 cut(s) 412, 601
BseLI CCNNNNNNNGG 1 cut(s) 840
BseMII CTCAG 3 cut(s) 23, 203, 635
BseNI ACTGG 1 cut(s) 222
BseSI GKGCMC 1 cut(s) 470
BseYI CCCAGC 3 cut(s) 137, 676, 720
BshFI GGCC 1 cut(s) 468
BshNI GGYRCC 1 cut(s) 360
BsiSI CCGG 1 cut(s) 85
BslI CCNNNNNNNGG 1 cut(s) 840
BsmAI GTCTC 1 cut(s) 192
BsmBI CGTCTC 1 cut(s) 192
BsnI GGCC 1 cut(s) 468
Bsp120I GGGCCC 1 cut(s) 466
Bsp1286I GDGCHC 1 cut(s) 470
Bsp143I GATC 3 cut(s) 242, 454, 634
Bsp19I CCATGG 1 cut(s) 402
BspACI CCGC 3 cut(s) 65, 470, 771
BspANI GGCC 1 cut(s) 468
BspCNI CTCAG 3 cut(s) 22, 202, 636
BspLI GGNNCC 3 cut(s) 183, 362, 468
BspOI GCTAGC 1 cut(s) 771
BspT107I GGYRCC 1 cut(s) 360
BsrI ACTGG 1 cut(s) 222
BssECI CCNNGG 1 cut(s) 402
BssMI GATC 3 cut(s) 242, 454, 634
BssT1I CCWWGG 1 cut(s) 402
Bst4CI ACNGT 4 cut(s) 186, 230, 665, 756
Bst6I CTCTTC 2 cut(s) 171, 625
BstC8I GCNNGC 2 cut(s) 470, 769
BstDEI CTNAG 4 cut(s) 9, 75, 189, 644
BstDSI CCRYGG 1 cut(s) 402
BstF5I GGATG 2 cut(s) 412, 601
BstKTI GATC 3 cut(s) 245, 457, 637
BstMAI GTCTC 1 cut(s) 192
BstMBI GATC 3 cut(s) 242, 454, 634
BstSCI CCNGG 1 cut(s) 83
BstSLI GKGCMC 1 cut(s) 470
BstXI CCANNNNNNTGG 1 cut(s) 445
BsuRI GGCC 1 cut(s) 468
BtgI CCRYGG 1 cut(s) 402
BtsCI GGATG 2 cut(s) 412, 601
BtsI GCAGTG 2 cut(s) 364, 623
BtsIMutI CAGTG 3 cut(s) 364, 623, 670
Cac8I GCNNGC 2 cut(s) 470, 769
CaiI CAGNNNCTG 1 cut(s) 676
Cfr13I GGNCC 2 cut(s) 466, 467
Csp6I GTAC 2 cut(s) 81, 361
CspCI CAANNNNNGTGG 2 cut(s) 8, 43
CviAII CATG 2 cut(s) 403, 815
CviQI GTAC 2 cut(s) 81, 361
DdeI CTNAG 4 cut(s) 9, 75, 189, 644
DpnI GATC 3 cut(s) 244, 456, 636
DpnII GATC 3 cut(s) 242, 454, 634
DraI TTTAAA 2 cut(s) 93, 696
Eam1104I CTCTTC 2 cut(s) 171, 625
EarI CTCTTC 2 cut(s) 171, 625
Eco130I CCWWGG 1 cut(s) 402
Eco24I GRGCYC 1 cut(s) 470
Eco57I CTGAAG 1 cut(s) 322
EcoT14I CCWWGG 1 cut(s) 402
EcoT38I GRGCYC 1 cut(s) 470
ErhI CCWWGG 1 cut(s) 402
Esp3I CGTCTC 1 cut(s) 192
FaeI CATG 2 cut(s) 406, 818
FatI CATG 2 cut(s) 402, 814
FauI CCCGC 1 cut(s) 477
FokI GGATG 2 cut(s) 419, 608
FriOI GRGCYC 1 cut(s) 470
FspBI CTAG 3 cut(s) 173, 659, 768
GsaI CCCAGC 3 cut(s) 141, 680, 724
HaeIII GGCC 1 cut(s) 468
HapII CCGG 1 cut(s) 85
Hin1II CATG 2 cut(s) 406, 818
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HinfI GANTC 4 cut(s) 193, 413, 547, 848
HpaI GTTAAC 1 cut(s) 388
HpaII CCGG 1 cut(s) 85
HphI GGTGA 3 cut(s) 149, 211, 468
Hpy166II GTNNAC 2 cut(s) 81, 388
Hpy188I TCNGA 1 cut(s) 192
Hpy188III TCNNGA 3 cut(s) 593, 638, 659
Hpy8I GTNNAC 2 cut(s) 81, 388
HpyAV CCTTC 6 cut(s) 290, 346, 376, 509, 773, 782
HpyCH4III ACNGT 4 cut(s) 186, 230, 665, 756
HpyCH4V TGCA 4 cut(s) 354, 565, 704, 814
HpyF3I CTNAG 4 cut(s) 9, 75, 189, 644
Hsp92II CATG 2 cut(s) 406, 818
KpnI GGTACC 1 cut(s) 364
KspAI GTTAAC 1 cut(s) 388
Kzo9I GATC 3 cut(s) 242, 454, 634
LweI GCATC 2 cut(s) 678, 691
MaeI CTAG 3 cut(s) 173, 659, 768
MaeIII GTNAC 2 cut(s) 224, 736
MalI GATC 3 cut(s) 244, 456, 636
MboI GATC 3 cut(s) 242, 454, 634
MboII GAAGA 3 cut(s) 188, 556, 642
MhlI GDGCHC 1 cut(s) 470
MluCI AATT 5 cut(s) 95, 302, 309, 483, 495
MlyI GAGTC 1 cut(s) 857
MmeI TCCRAC 1 cut(s) 825
MnlI CCTC 6 cut(s) 172, 263, 306, 424, 639, 727
MroXI GAANNNNTTC 1 cut(s) 792
MseI TTAA 5 cut(s) 92, 207, 239, 387, 695
MslI CAYNNNNRTG 1 cut(s) 443
MspA1I CMGCKG 1 cut(s) 248
MspI CCGG 1 cut(s) 85
MspR9I CCNGG 1 cut(s) 85
NciI CCSGG 1 cut(s) 85
NcoI CCATGG 1 cut(s) 402
NdeII GATC 3 cut(s) 242, 454, 634
NheI GCTAGC 1 cut(s) 767
NlaIII CATG 2 cut(s) 406, 818
NlaIV GGNNCC 3 cut(s) 183, 362, 468
NmuCI GTSAC 1 cut(s) 736
PdmI GAANNNNTTC 1 cut(s) 792
PfeI GAWTC 3 cut(s) 193, 413, 547
PleI GAGTC 1 cut(s) 856
PpsI GAGTC 1 cut(s) 856
PspFI CCCAGC 3 cut(s) 137, 676, 720
PspN4I GGNNCC 3 cut(s) 183, 362, 468
PspOMI GGGCCC 1 cut(s) 466
PspPI GGNCC 2 cut(s) 466, 467
PstNI CAGNNNCTG 1 cut(s) 676
PvuII CAGCTG 1 cut(s) 248
RsaI GTAC 2 cut(s) 82, 362
RsaNI GTAC 2 cut(s) 81, 361
RseI CAYNNNNRTG 1 cut(s) 443
SaqAI TTAA 5 cut(s) 92, 207, 239, 387, 695
Sau3AI GATC 3 cut(s) 242, 454, 634
Sau96I GGNCC 2 cut(s) 466, 467
SchI GAGTC 1 cut(s) 857
ScrFI CCNGG 1 cut(s) 85
SduI GDGCHC 1 cut(s) 470
SfaNI GCATC 2 cut(s) 678, 691
SmiMI CAYNNNNRTG 1 cut(s) 443
Sse9I AATT 5 cut(s) 95, 302, 309, 483, 495
SsiI CCGC 3 cut(s) 65, 470, 771
SspMI CTAG 3 cut(s) 173, 659, 768
StyD4I CCNGG 1 cut(s) 83
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 4 cut(s) 186, 230, 665, 756
TaqI TCGA 1 cut(s) 686
TasI AATT 5 cut(s) 95, 302, 309, 483, 495
TfiI GAWTC 3 cut(s) 193, 413, 547
Tru1I TTAA 5 cut(s) 92, 207, 239, 387, 695
Tru9I TTAA 5 cut(s) 92, 207, 239, 387, 695
TscAI CASTG 3 cut(s) 371, 623, 670
TseFI GTSAC 1 cut(s) 736
Tsp45I GTSAC 1 cut(s) 736
TspDTI ATGAA 3 cut(s) 295, 303, 748
TspRI CASTG 3 cut(s) 371, 623, 670
XbaI TCTAGA 1 cut(s) 658
XcmI CCANNNNNNNNNTGG 1 cut(s) 841
XmnI GAANNNNTTC 1 cut(s) 792
XspI CTAG 3 cut(s) 173, 659, 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.