FvH4_3g29630

Root cap

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002618
Physical Location & Seq
Forward (+)
22637427 .. 22639518
2092 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g29630.t1

Sequence Viewer

Length: 1509 bp
ATGGTTCATCCATCTACGGTGCGCCTGGGTGCTGTCTTGCTCATTCTGTTGTTCGCTGTTCTTTTAACTGAGGGAGCTCCTCAACCAAAGGCCAAGAAAGTCAAGTGTCAAGACAAGAAGTTCTACAAGTGTTATCACAAAGATCTCTATTGCCCTAAAGCATGCCTCCGTACTTGTGTTGTGGATTGTAAGAAATGCAAAGCTGTTTGTAAGGCCCCTCCTCCCCCACCACCGCCTCCACCACCGCCACCTCCGCCACCTCCGCCACCTCCGCCGAGACGTAGATGGCGCCAGTCACCCCCTCCTCCCCCAAAGAGGTATCACCATTCGCCACCTCCTCCTCCCAAGGCTTATCCTTCACCACCACCTGCCCCTTCAGTTGTTCCACCTCCCCCTCCTCCAACTACAACTCCACCTCCGTCACCTCCTGTTTCTACCCCGCCGTCACCACCATTGCCTCCTCCTACTACCCCATCACCACCGCCATCCCCTATTCCTACCCCTTCACCACCTTTGTCTACACCATCATCACCGCCACCCCTTACTCCTACTCCATCACCGCCGCCGCCCCCTACTTTTTCACCACCTTCTTTGTATCCTCCTCCACCACCGCCTTCGTCAGAGGCCGCCGGTGAAAAGAGAGTGAAATGCAAGAACAAGGACTACCCTCATTGCTACGGTCTAGAGATGAGTTGTCCTAGTGGGTGCCCTGAGCAATGCGAAGTTGACTGCGTTACATGCAGTCCCGTTTGTAATTGCAACAAACCAGGTGCTGTCTGCCAAGACCCCAGATTCGTTGGTGGTGATGGTATAACCTTCTATTTCCATGGCAAGCGAGACCAAGACTTCTGCCTCGTATCTGATTCCAACATCCACATCAACGCCCACTTCATCGGCAAAAGACACCAAAATATGAGGAGGGACTTCACTTGGGTCCAGTCTCTAGGTATCCTCTTTGACAACCGCAAGCTCTTCATAGGCGCCAAACCGACTTCCACTTGGGATGACTCTGTCGACCGCCTAGCCATAGTCATTGACGACGAAACCATCAGCCTTGCTGACAACGAGGGCGCATACTGGCGATCACAAGGACTCAAGATCACAAGAAGCAAGAACACAAATGCTGTAGAAATCGAAGCCGAAGGGAACTTCAAGATCAAAGCAGTAGTGGTGCCGATAACGGAGAAAGAGTCAGTGATACACAAGTACGGGATTACACAGGAGGATTGCTTTGCTCACCTTGACTTGAGCTTCAAGTTTTATGCCCTCAGTGGGGAAGTTAATGGTGTACTGGGACAGACGTACAGTGAGAACTATGTCAGCAGGGTTAAGATGGGTGTCGTGATGCCTGTTTTGGGTGGTGATAGAGAATTTTCATCGTCTAGCATCTTTGCCTCAGATTGTTCTGTTGCTAGGTTTAGCGGTGAATTTAGTGAAAAGTCCAAGTATGCTAGTACTATGAATTGTGCTAGTGGGATGGATGGTCGTGGAGTTGTTTGTAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Root_cap

Protein Analysis

503

Amino Acids

54.53

Weight (kDa)

8.91

Isoelectric Point (pI)

78.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Root_cap PF06830 417 - 473 5e-27 Root cap
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 376
Acc36I ACCTGC 1 cut(s) 376
AccB1I GGYRCC 4 cut(s) 288, 705, 980, 1171
AccI GTMKAC 2 cut(s) 518, 1014
AcsI RAATTY 2 cut(s) 1370, 1427
AcuI CTGAAG 1 cut(s) 360
AcyI GRCGYC 2 cut(s) 289, 981
AfaI GTAC 5 cut(s) 172, 1208, 1290, 1304, 1456
AfiI CCNNNNNNNGG 4 cut(s) 315, 1272, 1273, 1355
AgsI TTSAA 2 cut(s) 1153, 1255
AjnI CCWGG 2 cut(s) 24, 766
AluBI AGCT 4 cut(s) 77, 203, 970, 1251
AluI AGCT 4 cut(s) 77, 203, 970, 1251
Alw21I GWGCWC 1 cut(s) 79
Alw26I GTCTC 3 cut(s) 271, 831, 945
AlwNI CAGNNNCTG 1 cut(s) 773
AoxI GGCC 3 cut(s) 90, 213, 624
ApoI RAATTY 2 cut(s) 1370, 1427
ArsI GACNNNNNNTTYG 2 cut(s) 776, 808
AspLEI GCGC 4 cut(s) 24, 291, 983, 1073
AspS9I GGNCC 2 cut(s) 214, 934
AvaII GGWCC 1 cut(s) 934
BaeGI GKGCMC 1 cut(s) 710
BaeI ACNNNNGTAYC 2 cut(s) 1190, 1223
BanI GGYRCC 4 cut(s) 288, 705, 980, 1171
BanII GRGCYC 1 cut(s) 79
Bbv12I GWGCWC 1 cut(s) 79
BceAI ACGGC 1 cut(s) 427
BciT130I CCWGG 2 cut(s) 26, 768
BciVI GTATCC 2 cut(s) 606, 959
BcoDI GTCTC 3 cut(s) 271, 831, 945
BfaI CTAG 8 cut(s) 683, 699, 944, 1022, 1383, 1413, 1452, 1470
BfmI CTRYAG 1 cut(s) 1125
BfoI RGCGCY 2 cut(s) 292, 984
BfuAI ACCTGC 1 cut(s) 376
BfuI GTATCC 2 cut(s) 606, 959
BglII AGATCT 1 cut(s) 142
BisI GCNGC 3 cut(s) 563, 566, 627
BlsI GCNGC 3 cut(s) 564, 567, 628
BmcAI AGTACT 1 cut(s) 1456
Bme1390I CCNGG 2 cut(s) 26, 768
Bme18I GGWCC 1 cut(s) 934
BmgT120I GGNCC 2 cut(s) 214, 934
BmiI GGNNCC 6 cut(s) 216, 290, 707, 935, 982, 1173
BmrFI CCNGG 2 cut(s) 26, 768
BmrI ACTGGG 1 cut(s) 1301
BmsI GCATC 2 cut(s) 1335, 1395
BmuI ACTGGG 1 cut(s) 1301
Bpu10I CCTNAGC 1 cut(s) 711
BpuEI CTTGAG 2 cut(s) 1079, 1267
BsaHI GRCGYC 2 cut(s) 289, 981
BsaI GGTCTC 1 cut(s) 831
BsaJI CCNNGG 3 cut(s) 25, 345, 826
BsaXI ACNNNNNCTCC 8 cut(s) 394, 400, 424, 430, 535, 565, 1175, 1205
Bsc4I CCNNNNNNNGG 4 cut(s) 315, 1272, 1273, 1355
Bse118I RCCGGY 1 cut(s) 629
Bse1I ACTGG 4 cut(s) 292, 937, 1082, 1296
Bse3DI GCAATG 3 cut(s) 452, 670, 722
BseBI CCWGG 2 cut(s) 26, 768
BseDI CCNNGG 3 cut(s) 25, 345, 826
BseGI GGATG 6 cut(s) 7, 485, 870, 1009, 1482, 1486
BseLI CCNNNNNNNGG 4 cut(s) 315, 1272, 1273, 1355
BseMI GCAATG 3 cut(s) 452, 670, 722
BseMII CTCAG 4 cut(s) 60, 702, 1282, 1410
BseNI ACTGG 4 cut(s) 292, 937, 1082, 1296
BseRI GAGGAG 9 cut(s) 69, 210, 294, 327, 330, 387, 450, 591, 931
BseSI GKGCMC 1 cut(s) 710
Bsh1285I CGRYCG 1 cut(s) 1018
BshFI GGCC 3 cut(s) 92, 215, 626
BshNI GGYRCC 4 cut(s) 288, 705, 980, 1171
BsiEI CGRYCG 1 cut(s) 1018
BsiHKAI GWGCWC 1 cut(s) 79
BsiSI CCGG 1 cut(s) 630
BslFI GGGAC 3 cut(s) 729, 935, 1308
BslI CCNNNNNNNGG 4 cut(s) 315, 1272, 1273, 1355
BsmAI GTCTC 3 cut(s) 271, 831, 945
BsmBI CGTCTC 1 cut(s) 271
BsmFI GGGAC 3 cut(s) 729, 935, 1308
BsnI GGCC 3 cut(s) 92, 215, 626
Bso31I GGTCTC 1 cut(s) 831
Bsp1286I GDGCHC 2 cut(s) 79, 710
Bsp143I GATC 4 cut(s) 142, 1082, 1098, 1155
Bsp19I CCATGG 1 cut(s) 826
BspANI GGCC 3 cut(s) 92, 215, 626
BspCNI CTCAG 4 cut(s) 61, 703, 1281, 1409
BspLI GGNNCC 6 cut(s) 216, 290, 707, 935, 982, 1173
BspMI ACCTGC 1 cut(s) 376
BspQI GCTCTTC 1 cut(s) 977
BspT107I GGYRCC 4 cut(s) 288, 705, 980, 1171
BspTNI GGTCTC 1 cut(s) 831
BsrDI GCAATG 3 cut(s) 452, 670, 722
BsrFI RCCGGY 1 cut(s) 629
BsrI ACTGG 4 cut(s) 292, 937, 1082, 1296
BssAI RCCGGY 1 cut(s) 629
BssECI CCNNGG 3 cut(s) 25, 345, 826
BssMI GATC 4 cut(s) 142, 1082, 1098, 1155
BssNI GRCGYC 2 cut(s) 289, 981
BssT1I CCWWGG 2 cut(s) 345, 826
Bst2UI CCWGG 2 cut(s) 26, 768
Bst4CI ACNGT 3 cut(s) 19, 680, 1307
Bst6I CTCTTC 1 cut(s) 977
BstACI GRCGYC 2 cut(s) 289, 981
BstC8I GCNNGC 3 cut(s) 163, 833, 968
BstDEI CTNAG 4 cut(s) 69, 711, 1268, 1396
BstDSI CCRYGG 1 cut(s) 826
BstF5I GGATG 6 cut(s) 7, 485, 870, 1009, 1482, 1486
BstH2I RGCGCY 2 cut(s) 292, 984
BstHHI GCGC 4 cut(s) 24, 291, 983, 1073
BstKTI GATC 4 cut(s) 145, 1085, 1101, 1158
BstMAI GTCTC 3 cut(s) 271, 831, 945
BstMBI GATC 4 cut(s) 142, 1082, 1098, 1155
BstMCI CGRYCG 1 cut(s) 1018
BstMWI GCNNNNNNNGC 4 cut(s) 253, 262, 271, 738
BstNI CCWGG 2 cut(s) 26, 768
BstNSI RCATGY 2 cut(s) 165, 741
BstSCI CCNGG 2 cut(s) 24, 766
BstSFI CTRYAG 1 cut(s) 1125
BstSLI GKGCMC 1 cut(s) 710
BstX2I RGATCY 1 cut(s) 142
BstYI RGATCY 1 cut(s) 142
BsuI GTATCC 2 cut(s) 606, 959
BsuRI GGCC 3 cut(s) 92, 215, 626
BtgI CCRYGG 1 cut(s) 826
BtsCI GGATG 6 cut(s) 7, 485, 870, 1009, 1482, 1486
BtsIMutI CAGTG 3 cut(s) 1200, 1276, 1312
BveI ACCTGC 1 cut(s) 376
Cac8I GCNNGC 3 cut(s) 163, 833, 968
CaiI CAGNNNCTG 1 cut(s) 773
CfoI GCGC 4 cut(s) 24, 291, 983, 1073
Cfr10I RCCGGY 1 cut(s) 629
Cfr13I GGNCC 2 cut(s) 214, 934
CsiI ACCWGGT 1 cut(s) 766
Csp6I GTAC 5 cut(s) 171, 1207, 1289, 1303, 1455
CspCI CAANNNNNGTGG 2 cut(s) 573, 608
CviAII CATG 3 cut(s) 162, 738, 827
CviQI GTAC 5 cut(s) 171, 1207, 1289, 1303, 1455
DdeI CTNAG 4 cut(s) 69, 711, 1268, 1396
DinI GGCGCC 2 cut(s) 290, 982
DpnI GATC 4 cut(s) 144, 1084, 1100, 1157
DpnII GATC 4 cut(s) 142, 1082, 1098, 1155
Eam1104I CTCTTC 1 cut(s) 977
EarI CTCTTC 1 cut(s) 977
EciI GGCGGA 3 cut(s) 243, 252, 261
Ecl136II GAGCTC 1 cut(s) 77
Eco130I CCWWGG 2 cut(s) 345, 826
Eco24I GRGCYC 1 cut(s) 79
Eco31I GGTCTC 1 cut(s) 831
Eco47I GGWCC 1 cut(s) 934
Eco53kI GAGCTC 1 cut(s) 77
Eco57I CTGAAG 1 cut(s) 360
EcoICRI GAGCTC 1 cut(s) 77
EcoO109I RGGNCCY 1 cut(s) 214
EcoRII CCWGG 2 cut(s) 24, 766
EcoT14I CCWWGG 2 cut(s) 345, 826
EcoT38I GRGCYC 1 cut(s) 79
EgeI GGCGCC 2 cut(s) 290, 982
EheI GGCGCC 2 cut(s) 290, 982
ErhI CCWWGG 2 cut(s) 345, 826
Esp3I CGTCTC 1 cut(s) 271
FaeI CATG 3 cut(s) 165, 741, 830
FaqI GGGAC 3 cut(s) 729, 935, 1308
FatI CATG 3 cut(s) 161, 737, 826
FauI CCCGC 1 cut(s) 447
FblI GTMKAC 2 cut(s) 518, 1014
Fnu4HI GCNGC 3 cut(s) 563, 566, 627
FokI GGATG 5 cut(s) 472, 857, 1016, 1489, 1493
FriOI GRGCYC 1 cut(s) 79
Fsp4HI GCNGC 3 cut(s) 563, 566, 627
FspBI CTAG 8 cut(s) 683, 699, 944, 1022, 1383, 1413, 1452, 1470
GlaI GCGC 4 cut(s) 23, 290, 982, 1072
GluI GCNGC 3 cut(s) 563, 566, 627
HaeII RGCGCY 2 cut(s) 292, 984
HaeIII GGCC 3 cut(s) 92, 215, 626
HapII CCGG 1 cut(s) 630
HhaI GCGC 4 cut(s) 24, 291, 983, 1073
Hin1I GRCGYC 2 cut(s) 289, 981
Hin1II CATG 3 cut(s) 165, 741, 830
Hin6I GCGC 4 cut(s) 22, 289, 981, 1071
HinP1I GCGC 4 cut(s) 22, 289, 981, 1071
HincII GTYRAC 2 cut(s) 727, 1015
HindII GTYRAC 2 cut(s) 727, 1015
HinfI GANTC 5 cut(s) 792, 863, 1007, 1092, 1190
HpaII CCGG 1 cut(s) 630
Hpy166II GTNNAC 4 cut(s) 519, 727, 1015, 1289
Hpy188I TCNGA 3 cut(s) 622, 862, 1399
Hpy188III TCNNGA 5 cut(s) 110, 683, 1096, 1153, 1342
Hpy8I GTNNAC 4 cut(s) 519, 727, 1015, 1289
Hpy99I CGWCG 1 cut(s) 1043
HpyAV CCTTC 7 cut(s) 366, 384, 513, 597, 624, 826, 1136
HpyCH4III ACNGT 3 cut(s) 19, 680, 1307
HpyCH4IV ACGT 2 cut(s) 280, 1301
HpyCH4V TGCA 4 cut(s) 198, 651, 741, 759
HpyF10VI GCNNNNNNNGC 4 cut(s) 253, 262, 271, 738
HpyF3I CTNAG 4 cut(s) 69, 711, 1268, 1396
HpySE526I ACGT 2 cut(s) 280, 1301
Hsp92I GRCGYC 2 cut(s) 289, 981
Hsp92II CATG 3 cut(s) 165, 741, 830
HspAI GCGC 4 cut(s) 22, 289, 981, 1071
KasI GGCGCC 2 cut(s) 288, 980
Kzo9I GATC 4 cut(s) 142, 1082, 1098, 1155
LguI GCTCTTC 1 cut(s) 977
LmnI GCTCC 2 cut(s) 74, 82
LweI GCATC 2 cut(s) 1335, 1395
MabI ACCWGGT 1 cut(s) 766
MaeI CTAG 8 cut(s) 683, 699, 944, 1022, 1383, 1413, 1452, 1470
MaeII ACGT 2 cut(s) 280, 1301
MaeIII GTNAC 4 cut(s) 294, 420, 444, 733
MalI GATC 4 cut(s) 144, 1084, 1100, 1157
MboI GATC 4 cut(s) 142, 1082, 1098, 1155
MboII GAAGA 1 cut(s) 964
MflI RGATCY 1 cut(s) 142
MhlI GDGCHC 2 cut(s) 79, 710
MluCI AATT 4 cut(s) 754, 1370, 1427, 1462
Mly113I GGCGCC 2 cut(s) 289, 981
MlyI GAGTC 3 cut(s) 1001, 1086, 1199
MmeI TCCRAC 2 cut(s) 425, 891
MseI TTAA 3 cut(s) 65, 1281, 1329
MspI CCGG 1 cut(s) 630
MspR9I CCNGG 2 cut(s) 26, 768
MvaI CCWGG 2 cut(s) 26, 768
MwoI GCNNNNNNNGC 4 cut(s) 253, 262, 271, 738
NarI GGCGCC 2 cut(s) 289, 981
NcoI CCATGG 1 cut(s) 826
NdeII GATC 4 cut(s) 142, 1082, 1098, 1155
NlaIII CATG 3 cut(s) 165, 741, 830
NlaIV GGNNCC 6 cut(s) 216, 290, 707, 935, 982, 1173
NmeAIII GCCGAG 1 cut(s) 300
NmuCI GTSAC 3 cut(s) 294, 420, 444
NspI RCATGY 2 cut(s) 165, 741
PaeI GCATGC 1 cut(s) 165
PaqCI CACCTGC 1 cut(s) 376
PciSI GCTCTTC 1 cut(s) 977
PfeI GAWTC 2 cut(s) 792, 863
PflFI GACNNNGTC 1 cut(s) 1010
PkrI GCNGC 3 cut(s) 564, 567, 628
PleI GAGTC 3 cut(s) 1001, 1086, 1198
PluTI GGCGCC 2 cut(s) 292, 984
PpsI GAGTC 3 cut(s) 1001, 1086, 1198
Psp124BI GAGCTC 1 cut(s) 79
Psp6I CCWGG 2 cut(s) 24, 766
PspGI CCWGG 2 cut(s) 24, 766
PspN4I GGNNCC 6 cut(s) 216, 290, 707, 935, 982, 1173
PspPI GGNCC 2 cut(s) 214, 934
PsrI GAACNNNNNNTAC 2 cut(s) 647, 679
PstNI CAGNNNCTG 1 cut(s) 773
PsuI RGATCY 1 cut(s) 142
PsyI GACNNNGTC 1 cut(s) 1010
RsaI GTAC 5 cut(s) 172, 1208, 1290, 1304, 1456
RsaNI GTAC 5 cut(s) 171, 1207, 1289, 1303, 1455
SacI GAGCTC 1 cut(s) 79
SalI GTCGAC 1 cut(s) 1013
SapI GCTCTTC 1 cut(s) 977
SaqAI TTAA 3 cut(s) 65, 1281, 1329
SatI GCNGC 3 cut(s) 563, 566, 627
Sau3AI GATC 4 cut(s) 142, 1082, 1098, 1155
Sau96I GGNCC 2 cut(s) 214, 934
ScaI AGTACT 1 cut(s) 1456
SchI GAGTC 3 cut(s) 1001, 1086, 1199
ScrFI CCNGG 2 cut(s) 26, 768
SduI GDGCHC 2 cut(s) 79, 710
SexAI ACCWGGT 1 cut(s) 766
SfaNI GCATC 2 cut(s) 1335, 1395
SfcI CTRYAG 1 cut(s) 1125
SfoI GGCGCC 2 cut(s) 290, 982
SgrAI CRCCGGYG 1 cut(s) 629
SinI GGWCC 1 cut(s) 934
SmlI CTYRAG 2 cut(s) 1094, 1246
SmoI CTYRAG 2 cut(s) 1094, 1246
SphI GCATGC 1 cut(s) 165
Sse9I AATT 4 cut(s) 754, 1370, 1427, 1462
SspDI GGCGCC 2 cut(s) 288, 980
SspMI CTAG 8 cut(s) 683, 699, 944, 1022, 1383, 1413, 1452, 1470
SstI GAGCTC 1 cut(s) 79
StyD4I CCNGG 2 cut(s) 24, 766
StyI CCWWGG 2 cut(s) 345, 826
TaaI ACNGT 3 cut(s) 19, 680, 1307
TaiI ACGT 2 cut(s) 283, 1304
TaqI TCGA 2 cut(s) 1014, 1134
TasI AATT 4 cut(s) 754, 1370, 1427, 1462
TatI WGTACW 2 cut(s) 1288, 1454
TauI GCSGC 3 cut(s) 565, 568, 629
TfiI GAWTC 2 cut(s) 792, 863
Tru1I TTAA 3 cut(s) 65, 1281, 1329
Tru9I TTAA 3 cut(s) 65, 1281, 1329
TscAI CASTG 3 cut(s) 1200, 1276, 1312
TseFI GTSAC 3 cut(s) 294, 420, 444
Tsp45I GTSAC 3 cut(s) 294, 420, 444
TspDTI ATGAA 4 cut(s) 880, 964, 1365, 1475
TspGWI ACGGA 3 cut(s) 158, 408, 1196
TspRI CASTG 3 cut(s) 1200, 1276, 1312
Tth111I GACNNNGTC 1 cut(s) 1010
VpaK11BI GGWCC 1 cut(s) 934
XapI RAATTY 2 cut(s) 1370, 1427
XbaI TCTAGA 1 cut(s) 682
XceI RCATGY 2 cut(s) 165, 741
XmiI GTMKAC 2 cut(s) 518, 1014
XspI CTAG 8 cut(s) 683, 699, 944, 1022, 1383, 1413, 1452, 1470
ZrmI AGTACT 1 cut(s) 1456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.