FvH4_3g31090

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0005691
Physical Location & Seq
Reverse (-)
24844807 .. 24849791
4985 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g31090.t1

Sequence Viewer

Length: 324 bp
ATGAGTGTTATGAAGCGGTATGTGCTGCGATTGTTCATATCATTGAAGTACATCACAGCAAACGTCGTTGACCGAAATAATGGCCGGATTGTTGTAACAGCATCTACAATTGAACATTCGGTTAAGGGCTCACTCGAGTGTGGCCGCTCTTGCAATGCAAAAGCAGCATCAGTTGTTGGAGAAGTGCTCGCGATGCGACTTAAGGTGGAAGGTCTTGACCAGGGACAGGGACAAGGGATTCACATTAATGTAAATAATGAAGTTGAGAAGAAGGGTTTTAAAAACCGTACCAAGTTCCTTTTTCATGAAGCTTTCTGTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

108

Amino Acids

11.87

Weight (kDa)

9.55

Isoelectric Point (pI)

25.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 4 - 73 5.2e-08 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 147
AccII CGCG 1 cut(s) 191
AciI CCGC 2 cut(s) 16, 145
AcoI YGGCCR 2 cut(s) 82, 142
AfaI GTAC 2 cut(s) 50, 289
AfiI CCNNNNNNNGG 1 cut(s) 226
AflII CTTAAG 1 cut(s) 200
AgsI TTSAA 2 cut(s) 46, 113
AjnI CCWGG 1 cut(s) 219
AleI CACNNNNGTG 1 cut(s) 136
AluBI AGCT 1 cut(s) 311
AluI AGCT 1 cut(s) 311
Alw21I GWGCWC 1 cut(s) 189
Ama87I CYCGRG 1 cut(s) 134
AoxI GGCC 2 cut(s) 82, 142
ApeKI GCWGC 2 cut(s) 25, 164
AseI ATTAAT 1 cut(s) 246
AvaI CYCGRG 1 cut(s) 134
BanII GRGCYC 1 cut(s) 131
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 2 cut(s) 12, 176
BciT130I CCWGG 1 cut(s) 221
BfaI CTAG 1 cut(s) 322
BfrI CTTAAG 1 cut(s) 200
BisI GCNGC 3 cut(s) 26, 145, 165
BlsI GCNGC 3 cut(s) 27, 146, 166
Bme1390I CCNGG 1 cut(s) 221
BmeT110I CYCGRG 1 cut(s) 134
BmrFI CCNGG 1 cut(s) 221
BmsI GCATC 3 cut(s) 110, 176, 183
BplI GAGNNNNNCTC 2 cut(s) 171, 203
BsaJI CCNNGG 1 cut(s) 220
Bsc4I CCNNNNNNNGG 1 cut(s) 226
Bse3DI GCAATG 1 cut(s) 160
BseBI CCWGG 1 cut(s) 221
BseDI CCNNGG 1 cut(s) 220
BseLI CCNNNNNNNGG 1 cut(s) 226
BseMI GCAATG 1 cut(s) 160
BseXI GCAGC 2 cut(s) 12, 176
Bsh1236I CGCG 1 cut(s) 191
BshFI GGCC 2 cut(s) 84, 144
BsiHKAI GWGCWC 1 cut(s) 189
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 1 cut(s) 85
BslFI GGGAC 2 cut(s) 237, 243
BslI CCNNNNNNNGG 1 cut(s) 226
BsmFI GGGAC 2 cut(s) 237, 243
BsnI GGCC 2 cut(s) 84, 144
BsoBI CYCGRG 1 cut(s) 134
Bsp1286I GDGCHC 2 cut(s) 131, 189
Bsp68I TCGCGA 1 cut(s) 191
BspACI CCGC 2 cut(s) 16, 145
BspANI GGCC 2 cut(s) 84, 144
BspFNI CGCG 1 cut(s) 191
BspHI TCATGA 1 cut(s) 304
BspTI CTTAAG 1 cut(s) 200
BsrBI CCGCTC 1 cut(s) 147
BsrDI GCAATG 1 cut(s) 160
BssECI CCNNGG 1 cut(s) 220
Bst2UI CCWGG 1 cut(s) 221
Bst4CI ACNGT 1 cut(s) 287
BstAFI CTTAAG 1 cut(s) 200
BstC8I GCNNGC 1 cut(s) 189
BstFNI CGCG 1 cut(s) 191
BstMWI GCNNNNNNNGC 4 cut(s) 22, 150, 164, 193
BstNI CCWGG 1 cut(s) 221
BstSCI CCNGG 1 cut(s) 219
BstUI CGCG 1 cut(s) 191
BstV1I GCAGC 2 cut(s) 12, 176
BsuRI GGCC 2 cut(s) 84, 144
BtgZI GCGATG 1 cut(s) 206
BtuMI TCGCGA 1 cut(s) 191
Cac8I GCNNGC 1 cut(s) 189
CciI TCATGA 1 cut(s) 304
Csp6I GTAC 2 cut(s) 49, 288
CviAII CATG 1 cut(s) 305
CviJI RGCY 4 cut(s) 84, 129, 144, 311
CviKI_1 RGCY 4 cut(s) 84, 129, 144, 311
CviQI GTAC 2 cut(s) 49, 288
DraI TTTAAA 1 cut(s) 280
EaeI YGGCCR 2 cut(s) 82, 142
Eco24I GRGCYC 1 cut(s) 131
Eco88I CYCGRG 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 219
EcoT38I GRGCYC 1 cut(s) 131
FaeI CATG 1 cut(s) 308
FaiI YATR 4 cut(s) 11, 21, 38, 306
FaqI GGGAC 2 cut(s) 237, 243
FatI CATG 1 cut(s) 304
Fnu4HI GCNGC 3 cut(s) 26, 145, 165
FriOI GRGCYC 1 cut(s) 131
Fsp4HI GCNGC 3 cut(s) 26, 145, 165
FspBI CTAG 1 cut(s) 322
GluI GCNGC 3 cut(s) 26, 145, 165
HaeIII GGCC 2 cut(s) 84, 144
HapII CCGG 1 cut(s) 85
Hin1II CATG 1 cut(s) 308
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HindIII AAGCTT 1 cut(s) 309
HinfI GANTC 1 cut(s) 238
HpaII CCGG 1 cut(s) 85
Hpy166II GTNNAC 1 cut(s) 70
Hpy188III TCNNGA 3 cut(s) 190, 215, 305
Hpy8I GTNNAC 1 cut(s) 70
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 2 cut(s) 203, 265
HpyCH4III ACNGT 1 cut(s) 287
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 2 cut(s) 153, 158
HpyF10VI GCNNNNNNNGC 4 cut(s) 22, 150, 164, 193
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 1 cut(s) 308
LpnPI CCDG 4 cut(s) 98, 206, 212, 233
Lsp1109I GCAGC 2 cut(s) 12, 176
LweI GCATC 3 cut(s) 110, 176, 183
MaeI CTAG 1 cut(s) 322
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 1 cut(s) 94
MbiI CCGCTC 1 cut(s) 147
MboII GAAGA 1 cut(s) 280
MfeI CAATTG 1 cut(s) 108
MhlI GDGCHC 2 cut(s) 131, 189
MluCI AATT 1 cut(s) 108
MmeI TCCRAC 1 cut(s) 157
MseI TTAA 4 cut(s) 123, 201, 246, 279
MslI CAYNNNNRTG 2 cut(s) 136, 246
MspCI CTTAAG 1 cut(s) 200
MspI CCGG 1 cut(s) 85
MspR9I CCNGG 1 cut(s) 221
MunI CAATTG 1 cut(s) 108
MvaI CCWGG 1 cut(s) 221
MvnI CGCG 1 cut(s) 191
MwoI GCNNNNNNNGC 4 cut(s) 22, 150, 164, 193
NlaIII CATG 1 cut(s) 308
NruI TCGCGA 1 cut(s) 191
OliI CACNNNNGTG 1 cut(s) 136
PaeR7I CTCGAG 1 cut(s) 134
PagI TCATGA 1 cut(s) 304
PfeI GAWTC 1 cut(s) 238
PkrI GCNGC 3 cut(s) 27, 146, 166
PshBI ATTAAT 1 cut(s) 246
Psp6I CCWGG 1 cut(s) 219
PspGI CCWGG 1 cut(s) 219
PspXI VCTCGAGB 1 cut(s) 134
RruI TCGCGA 1 cut(s) 191
RsaI GTAC 2 cut(s) 50, 289
RsaNI GTAC 2 cut(s) 49, 288
RseI CAYNNNNRTG 2 cut(s) 136, 246
SaqAI TTAA 4 cut(s) 123, 201, 246, 279
SatI GCNGC 3 cut(s) 26, 145, 165
ScrFI CCNGG 1 cut(s) 221
SduI GDGCHC 2 cut(s) 131, 189
SetI ASST 4 cut(s) 66, 207, 214, 313
SfaNI GCATC 3 cut(s) 110, 176, 183
Sfr274I CTCGAG 1 cut(s) 134
SlaI CTCGAG 1 cut(s) 134
SmiMI CAYNNNNRTG 2 cut(s) 136, 246
SmlI CTYRAG 2 cut(s) 134, 200
SmoI CTYRAG 2 cut(s) 134, 200
Sse9I AATT 1 cut(s) 108
SsiI CCGC 2 cut(s) 16, 145
SspMI CTAG 1 cut(s) 322
StyD4I CCNGG 1 cut(s) 219
TaaI ACNGT 1 cut(s) 287
TaiI ACGT 1 cut(s) 66
TaqI TCGA 1 cut(s) 135
TaqII GACCGA 1 cut(s) 87
TasI AATT 1 cut(s) 108
TatI WGTACW 1 cut(s) 48
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 1 cut(s) 238
Tru1I TTAA 4 cut(s) 123, 201, 246, 279
Tru9I TTAA 4 cut(s) 123, 201, 246, 279
TseI GCWGC 2 cut(s) 25, 164
TspDTI ATGAA 5 cut(s) 25, 26, 273, 293, 321
Vha464I CTTAAG 1 cut(s) 200
VspI ATTAAT 1 cut(s) 246
XhoI CTCGAG 1 cut(s) 134
XspI CTAG 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.