Rh5DG402800

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0005691
Physical Location & Seq
Reverse (-)
63826177 .. 63830220
4044 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG402800.1

Sequence Viewer

Length: 681 bp
ATGATTGGGCCGGGCCGGACCTTGCGAAGAAGAGAAGGCGATCGGAGAAGAGAGCGCGAGGGCGGTGGGACTCAGATCTGGGATCGGTGGTGGTTCTCAGGCGGGTTGGGTCGTGGCCTGACACGTGTTCAGGATCTATCGGCAGTCCCAGACTATGGCTTCGGGTTACGTATTCAATTTGCTATTGATTTGTCTCCACGTGACACGGCACCGTTTCGGTTCAGCCCAAAACGCAGCGTTTTCGGTTCAGCCCAAAACGCAGCGTTTCGGTCTCTTTGGCAGCATAAACACCCTGCTCCTTGCAATTCTGTCTCTATCGCTTTTACAAACTCAGCAGTCTGGTTATGTTTTTGGGAGTGTCCAAGAATGAGTCTTATGAAGCGGTATGTGCTGCGGTTGTTCATATCATTGAAGTACATCACAGCAAACGTCGTTGACAGAAATAATGGCCGGATTGTTGTAACAGCATCTACAATTGAACATTCAGTTAAGGGCTCGCTCGAGTGTGGCCGCTCTTGCAACGCAAAGGCGGCAGCAGTTGTTGGAGAGGTGCTCGCTATGCGACTTAAGGTTGAAGGTCTTGACCAGGGACAGGGACGAGGGATTCATGTCAATGTAAATAATGAAGTTGAGAAGAAGGGTTTTAAGAACCGTACCAAGAAGGATTTGAGCTGGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

226

Amino Acids

25.45

Weight (kDa)

10.5

Isoelectric Point (pI)

46.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 127 - 195 3e-07 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 208
AccB7I CCANNNNNTGG 1 cut(s) 155
AccBSI CCGCTC 1 cut(s) 513
AccII CGCG 1 cut(s) 57
AciI CCGC 6 cut(s) 63, 102, 382, 394, 511, 530
AclWI GGATC 2 cut(s) 90, 141
AcoI YGGCCR 2 cut(s) 448, 508
AcvI CACGTG 2 cut(s) 125, 200
AfaI GTAC 2 cut(s) 416, 655
AfiI CCNNNNNNNGG 2 cut(s) 155, 592
AflII CTTAAG 1 cut(s) 566
AflIII ACRYGT 2 cut(s) 122, 124
AgsI TTSAA 4 cut(s) 176, 412, 479, 575
AjnI CCWGG 1 cut(s) 585
AluBI AGCT 1 cut(s) 672
AluI AGCT 1 cut(s) 672
Alw21I GWGCWC 1 cut(s) 555
Alw26I GTCTC 3 cut(s) 198, 276, 316
AlwI GGATC 2 cut(s) 90, 141
Ama87I CYCGRG 1 cut(s) 500
AoxI GGCC 5 cut(s) 8, 13, 115, 448, 508
ApeKI GCWGC 5 cut(s) 234, 260, 280, 391, 533
ArsI GACNNNNNNTTYG 2 cut(s) 143, 175
AspLEI GCGC 1 cut(s) 57
AspS9I GGNCC 3 cut(s) 8, 13, 18
AsuC2I CCSGG 1 cut(s) 12
AvaI CYCGRG 1 cut(s) 500
AvaII GGWCC 1 cut(s) 18
BanI GGYRCC 1 cut(s) 208
BanII GRGCYC 1 cut(s) 497
BbrPI CACGTG 2 cut(s) 125, 200
Bbv12I GWGCWC 1 cut(s) 555
BbvI GCAGC 5 cut(s) 246, 272, 292, 378, 545
BceAI ACGGC 1 cut(s) 222
BcgI CGANNNNNNTGC 2 cut(s) 223, 257
BciT130I CCWGG 1 cut(s) 587
BcnI CCSGG 1 cut(s) 12
BcoDI GTCTC 3 cut(s) 198, 276, 316
BfrI CTTAAG 1 cut(s) 566
BglII AGATCT 1 cut(s) 75
BisI GCNGC 7 cut(s) 235, 261, 281, 392, 511, 531, 534
BlsI GCNGC 7 cut(s) 236, 262, 282, 393, 512, 532, 535
Bme1390I CCNGG 2 cut(s) 12, 587
Bme18I GGWCC 1 cut(s) 18
BmeT110I CYCGRG 1 cut(s) 500
BmgT120I GGNCC 3 cut(s) 8, 13, 18
BmiI GGNNCC 1 cut(s) 210
BmrFI CCNGG 2 cut(s) 12, 587
BmsI GCATC 1 cut(s) 476
BplI GAGNNNNNCTC 2 cut(s) 537, 569
BpuMI CCSGG 1 cut(s) 12
BsaAI YACGTR 3 cut(s) 125, 170, 200
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 586
Bsc4I CCNNNNNNNGG 2 cut(s) 155, 592
BseBI CCWGG 1 cut(s) 587
BseDI CCNNGG 1 cut(s) 586
BseLI CCNNNNNNNGG 2 cut(s) 155, 592
BseMII CTCAG 3 cut(s) 86, 111, 345
BseXI GCAGC 5 cut(s) 246, 272, 292, 378, 545
Bsh1236I CGCG 1 cut(s) 57
Bsh1285I CGRYCG 1 cut(s) 43
BshFI GGCC 5 cut(s) 10, 15, 117, 450, 510
BshNI GGYRCC 1 cut(s) 208
BsiEI CGRYCG 1 cut(s) 43
BsiHKAI GWGCWC 1 cut(s) 555
BsiHKCI CYCGRG 1 cut(s) 500
BsiSI CCGG 3 cut(s) 11, 16, 451
BslFI GGGAC 4 cut(s) 82, 131, 603, 609
BslI CCNNNNNNNGG 2 cut(s) 155, 592
BsmAI GTCTC 3 cut(s) 198, 276, 316
BsmFI GGGAC 4 cut(s) 82, 131, 603, 609
BsnI GGCC 5 cut(s) 10, 15, 117, 450, 510
Bso31I GGTCTC 1 cut(s) 276
BsoBI CYCGRG 1 cut(s) 500
Bsp1286I GDGCHC 2 cut(s) 497, 555
Bsp143I GATC 4 cut(s) 40, 75, 82, 133
BspACI CCGC 6 cut(s) 63, 102, 382, 394, 511, 530
BspANI GGCC 5 cut(s) 10, 15, 117, 450, 510
BspCNI CTCAG 3 cut(s) 85, 110, 344
BspFNI CGCG 1 cut(s) 57
BspLI GGNNCC 1 cut(s) 210
BspPI GGATC 2 cut(s) 90, 141
BspT107I GGYRCC 1 cut(s) 208
BspTI CTTAAG 1 cut(s) 566
BspTNI GGTCTC 1 cut(s) 276
BsrBI CCGCTC 1 cut(s) 513
BssECI CCNNGG 1 cut(s) 586
BssMI GATC 4 cut(s) 40, 75, 82, 133
Bst2UI CCWGG 1 cut(s) 587
Bst4CI ACNGT 2 cut(s) 213, 653
Bst6I CTCTTC 2 cut(s) 25, 43
BstAFI CTTAAG 1 cut(s) 566
BstBAI YACGTR 3 cut(s) 125, 170, 200
BstC8I GCNNGC 2 cut(s) 497, 555
BstDEI CTNAG 3 cut(s) 72, 97, 331
BstFNI CGCG 1 cut(s) 57
BstHHI GCGC 1 cut(s) 57
BstKTI GATC 4 cut(s) 43, 78, 85, 136
BstMAI GTCTC 3 cut(s) 198, 276, 316
BstMBI GATC 4 cut(s) 40, 75, 82, 133
BstMCI CGRYCG 1 cut(s) 43
BstMWI GCNNNNNNNGC 6 cut(s) 231, 257, 388, 516, 530, 559
BstNI CCWGG 1 cut(s) 587
BstSCI CCNGG 2 cut(s) 10, 585
BstSNI TACGTA 1 cut(s) 170
BstUI CGCG 1 cut(s) 57
BstV1I GCAGC 5 cut(s) 246, 272, 292, 378, 545
BstX2I RGATCY 2 cut(s) 75, 133
BstYI RGATCY 2 cut(s) 75, 133
BsuRI GGCC 5 cut(s) 10, 15, 117, 450, 510
Cac8I GCNNGC 2 cut(s) 497, 555
CfoI GCGC 1 cut(s) 57
Cfr13I GGNCC 3 cut(s) 8, 13, 18
Csp6I GTAC 2 cut(s) 415, 654
CviAII CATG 1 cut(s) 608
CviQI GTAC 2 cut(s) 415, 654
DdeI CTNAG 3 cut(s) 72, 97, 331
DpnI GATC 4 cut(s) 42, 77, 84, 135
DpnII GATC 4 cut(s) 40, 75, 82, 133
EaeI YGGCCR 2 cut(s) 448, 508
Eam1104I CTCTTC 2 cut(s) 25, 43
EarI CTCTTC 2 cut(s) 25, 43
Eco105I TACGTA 1 cut(s) 170
Eco24I GRGCYC 1 cut(s) 497
Eco31I GGTCTC 1 cut(s) 276
Eco47I GGWCC 1 cut(s) 18
Eco72I CACGTG 2 cut(s) 125, 200
Eco88I CYCGRG 1 cut(s) 500
EcoRII CCWGG 1 cut(s) 585
EcoT38I GRGCYC 1 cut(s) 497
FaeI CATG 1 cut(s) 611
FaiI YATR 9 cut(s) 156, 285, 346, 377, 387, 404, 560, 609, 679
FaqI GGGAC 4 cut(s) 82, 131, 603, 609
FatI CATG 1 cut(s) 607
FauI CCCGC 1 cut(s) 95
Fnu4HI GCNGC 7 cut(s) 235, 261, 281, 392, 511, 531, 534
FriOI GRGCYC 1 cut(s) 497
Fsp4HI GCNGC 7 cut(s) 235, 261, 281, 392, 511, 531, 534
GlaI GCGC 1 cut(s) 56
GluI GCNGC 7 cut(s) 235, 261, 281, 392, 511, 531, 534
HaeIII GGCC 5 cut(s) 10, 15, 117, 450, 510
HapII CCGG 3 cut(s) 11, 16, 451
HhaI GCGC 1 cut(s) 57
Hin1II CATG 1 cut(s) 611
Hin6I GCGC 1 cut(s) 55
HinP1I GCGC 1 cut(s) 55
HincII GTYRAC 1 cut(s) 436
HindII GTYRAC 1 cut(s) 436
HinfI GANTC 3 cut(s) 70, 370, 604
HpaII CCGG 3 cut(s) 11, 16, 451
Hpy166II GTNNAC 1 cut(s) 436
Hpy188I TCNGA 2 cut(s) 45, 75
Hpy188III TCNNGA 2 cut(s) 131, 581
Hpy8I GTNNAC 1 cut(s) 436
Hpy99I CGWCG 1 cut(s) 434
HpyAV CCTTC 4 cut(s) 29, 569, 631, 655
HpyCH4III ACNGT 2 cut(s) 213, 653
HpyCH4IV ACGT 4 cut(s) 124, 169, 199, 429
HpyCH4V TGCA 2 cut(s) 303, 519
HpyF10VI GCNNNNNNNGC 6 cut(s) 231, 257, 388, 516, 530, 559
HpyF3I CTNAG 3 cut(s) 72, 97, 331
HpySE526I ACGT 4 cut(s) 124, 169, 199, 429
Hsp92II CATG 1 cut(s) 611
HspAI GCGC 1 cut(s) 55
Kzo9I GATC 4 cut(s) 40, 75, 82, 133
LmnI GCTCC 1 cut(s) 301
Lsp1109I GCAGC 5 cut(s) 246, 272, 292, 378, 545
LweI GCATC 1 cut(s) 476
MaeII ACGT 4 cut(s) 124, 169, 199, 429
MaeIII GTNAC 3 cut(s) 165, 200, 460
MalI GATC 4 cut(s) 42, 77, 84, 135
MbiI CCGCTC 1 cut(s) 513
MboI GATC 4 cut(s) 40, 75, 82, 133
MboII GAAGA 4 cut(s) 39, 42, 60, 646
MfeI CAATTG 1 cut(s) 474
MflI RGATCY 2 cut(s) 75, 133
MhlI GDGCHC 2 cut(s) 497, 555
MluCI AATT 3 cut(s) 176, 304, 474
MlyI GAGTC 2 cut(s) 64, 379
MmeI TCCRAC 1 cut(s) 523
MnlI CCTC 3 cut(s) 52, 541, 593
MseI TTAA 3 cut(s) 489, 567, 645
MslI CAYNNNNRTG 1 cut(s) 612
MspCI CTTAAG 1 cut(s) 566
MspI CCGG 3 cut(s) 11, 16, 451
MspR9I CCNGG 2 cut(s) 12, 587
MunI CAATTG 1 cut(s) 474
MvaI CCWGG 1 cut(s) 587
MvnI CGCG 1 cut(s) 57
MwoI GCNNNNNNNGC 6 cut(s) 231, 257, 388, 516, 530, 559
NciI CCSGG 1 cut(s) 12
NdeII GATC 4 cut(s) 40, 75, 82, 133
NlaIII CATG 1 cut(s) 611
NlaIV GGNNCC 1 cut(s) 210
NmuCI GTSAC 1 cut(s) 200
PaeR7I CTCGAG 1 cut(s) 500
PfeI GAWTC 1 cut(s) 604
PflMI CCANNNNNTGG 1 cut(s) 155
PkrI GCNGC 7 cut(s) 236, 262, 282, 393, 512, 532, 535
Ple19I CGATCG 1 cut(s) 43
PleI GAGTC 2 cut(s) 64, 378
PmaCI CACGTG 2 cut(s) 125, 200
PmlI CACGTG 2 cut(s) 125, 200
PpsI GAGTC 2 cut(s) 64, 378
Ppu21I YACGTR 3 cut(s) 125, 170, 200
Psp6I CCWGG 1 cut(s) 585
PspCI CACGTG 2 cut(s) 125, 200
PspGI CCWGG 1 cut(s) 585
PspN4I GGNNCC 1 cut(s) 210
PspPI GGNCC 3 cut(s) 8, 13, 18
PspXI VCTCGAGB 1 cut(s) 500
PsuI RGATCY 2 cut(s) 75, 133
PvuI CGATCG 1 cut(s) 43
RsaI GTAC 2 cut(s) 416, 655
RsaNI GTAC 2 cut(s) 415, 654
RseI CAYNNNNRTG 1 cut(s) 612
SaqAI TTAA 3 cut(s) 489, 567, 645
SatI GCNGC 7 cut(s) 235, 261, 281, 392, 511, 531, 534
Sau3AI GATC 4 cut(s) 40, 75, 82, 133
Sau96I GGNCC 3 cut(s) 8, 13, 18
SchI GAGTC 2 cut(s) 64, 379
ScrFI CCNGG 2 cut(s) 12, 587
SduI GDGCHC 2 cut(s) 497, 555
SetI ASST 9 cut(s) 23, 127, 172, 202, 432, 552, 573, 580, 674
SfaNI GCATC 1 cut(s) 476
Sfr274I CTCGAG 1 cut(s) 500
SinI GGWCC 1 cut(s) 18
SlaI CTCGAG 1 cut(s) 500
SmiMI CAYNNNNRTG 1 cut(s) 612
SmlI CTYRAG 2 cut(s) 500, 566
SmoI CTYRAG 2 cut(s) 500, 566
SnaBI TACGTA 1 cut(s) 170
Sse9I AATT 3 cut(s) 176, 304, 474
SsiI CCGC 6 cut(s) 63, 102, 382, 394, 511, 530
StyD4I CCNGG 2 cut(s) 10, 585
TaaI ACNGT 2 cut(s) 213, 653
TaiI ACGT 4 cut(s) 127, 172, 202, 432
TaqI TCGA 1 cut(s) 501
TaqII GACCGA 1 cut(s) 258
TasI AATT 3 cut(s) 176, 304, 474
TatI WGTACW 1 cut(s) 414
TauI GCSGC 2 cut(s) 513, 533
TfiI GAWTC 1 cut(s) 604
Tru1I TTAA 3 cut(s) 489, 567, 645
Tru9I TTAA 3 cut(s) 489, 567, 645
TseFI GTSAC 1 cut(s) 200
TseI GCWGC 5 cut(s) 234, 260, 280, 391, 533
Tsp45I GTSAC 1 cut(s) 200
TspDTI ATGAA 4 cut(s) 391, 392, 596, 639
Van91I CCANNNNNTGG 1 cut(s) 155
Vha464I CTTAAG 1 cut(s) 566
VpaK11BI GGWCC 1 cut(s) 18
XhoI CTCGAG 1 cut(s) 500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.