MD00G1033500.v1.1

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0005691
Physical Location & Seq
Reverse (-)
5789826 .. 5793068
3243 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1033500.v1.1.491

Sequence Viewer

Length: 723 bp
ATGTGGTTTGATGATGCCATTTGTCACTTCACTCCGACCTCCGTGCTGCAACGGGAGAGAAGGGGGAGAGCACTAAGGAGAGTTTGGGCTCCAATTCCAAAGACAATATTTGGGTTTGGGTGTCCATTACTTGTCAAACTTGGACGCAGTAGCCCAGTAACCACCACAGCCCTGCAAGAGCCCGTTACCCACACCAGCCCACCGTTGGCTTTCCTCCTCCTCCTCCTCGTCCTCCTGCTTTCCTGGTTCGGCTCGCACGGTCACACTCACTCCTACGCTTCCACCCGGCCAAGCTTTCATCTTTGTTCCCGACAGGACTGCCCCAGAATGACGGTTGTGAAGCGATATGTGCTACGGCTGTTCATATCATTGAAGTACATTACAGCAAACGTAGTGGACAGAAATAATGGCCGAATTGTTGTGTCAGCATCTACGGTTGAACATTCAATCAAGGGCTCACTCGAATGTGGAAGGTCTTGCAATGCAAAGGCAGCGGCAGTTGTTGGAGAGGTGTTGGCTAGGCGACTCAAGGTGGAAGGTCTTGGCGAGGGACAGGGACGAGGGATTCATGTCAATGTAAATAACGAAGTTGAGAAAAAGGGTTTTAAGAACCGCACCAAGATTTGGGCTATTGTCAATGCTCTTAAGGACAATGGAGTCAAACTCATTCTTGACGGTAGTGATGAAAATACTTCTCAGCCAAAAAGGAAGGATTTGCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

241

Amino Acids

26.69

Weight (kDa)

10.2

Isoelectric Point (pI)

38.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 114 - 181 7.8e-08 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 656
AccB7I CCANNNNNTGG 1 cut(s) 624
AciI CCGC 2 cut(s) 494, 613
AcoI YGGCCR 2 cut(s) 287, 409
AfaI GTAC 1 cut(s) 377
AfiI CCNNNNNNNGG 2 cut(s) 205, 624
AflII CTTAAG 1 cut(s) 644
AgsI TTSAA 3 cut(s) 373, 440, 447
AjnI CCWGG 1 cut(s) 242
AluBI AGCT 1 cut(s) 294
AluI AGCT 1 cut(s) 294
Alw21I GWGCWC 1 cut(s) 73
AoxI GGCC 2 cut(s) 287, 409
ApeKI GCWGC 2 cut(s) 46, 491
AspLEI GCGC 1 cut(s) 720
AsuC2I CCSGG 1 cut(s) 286
BanII GRGCYC 3 cut(s) 91, 183, 458
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 2 cut(s) 33, 503
BceAI ACGGC 1 cut(s) 371
BcgI CGANNNNNNTGC 4 cut(s) 25, 59, 300, 334
BciT130I CCWGG 1 cut(s) 244
BcnI CCSGG 1 cut(s) 286
BfaI CTAG 1 cut(s) 519
BfrI CTTAAG 1 cut(s) 644
BisI GCNGC 3 cut(s) 47, 492, 495
BlsI GCNGC 3 cut(s) 48, 493, 496
Bme1390I CCNGG 2 cut(s) 244, 286
BmiI GGNNCC 1 cut(s) 90
BmrFI CCNGG 2 cut(s) 244, 286
BmrI ACTGGG 1 cut(s) 149
BmsI GCATC 2 cut(s) 4, 437
BmuI ACTGGG 1 cut(s) 149
BplI GAGNNNNNCTC 2 cut(s) 648, 680
BpuEI CTTGAG 1 cut(s) 512
BpuMI CCSGG 1 cut(s) 286
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bsc4I CCNNNNNNNGG 2 cut(s) 205, 624
Bse1I ACTGG 1 cut(s) 155
Bse3DI GCAATG 1 cut(s) 487
BseBI CCWGG 1 cut(s) 244
BseLI CCNNNNNNNGG 2 cut(s) 205, 624
BseMI GCAATG 1 cut(s) 487
BseMII CTCAG 1 cut(s) 710
BseNI ACTGG 1 cut(s) 155
BseRI GAGGAG 4 cut(s) 206, 209, 212, 215
BseXI GCAGC 2 cut(s) 33, 503
BshFI GGCC 2 cut(s) 289, 411
BsiHKAI GWGCWC 1 cut(s) 73
BsiSI CCGG 1 cut(s) 286
BslFI GGGAC 2 cut(s) 564, 570
BslI CCNNNNNNNGG 2 cut(s) 205, 624
BsmFI GGGAC 2 cut(s) 564, 570
BsnI GGCC 2 cut(s) 289, 411
Bsp1286I GDGCHC 4 cut(s) 73, 91, 183, 458
BspACI CCGC 2 cut(s) 494, 613
BspANI GGCC 2 cut(s) 289, 411
BspCNI CTCAG 1 cut(s) 709
BspLI GGNNCC 1 cut(s) 90
BspTI CTTAAG 1 cut(s) 644
BsrDI GCAATG 1 cut(s) 487
BsrI ACTGG 1 cut(s) 155
Bst2UI CCWGG 1 cut(s) 244
Bst4CI ACNGT 5 cut(s) 204, 260, 334, 436, 677
BstAFI CTTAAG 1 cut(s) 644
BstC8I GCNNGC 1 cut(s) 254
BstDEI CTNAG 2 cut(s) 74, 696
BstHHI GCGC 1 cut(s) 720
BstMWI GCNNNNNNNGC 2 cut(s) 349, 491
BstNI CCWGG 1 cut(s) 244
BstSCI CCNGG 2 cut(s) 242, 284
BstV1I GCAGC 2 cut(s) 33, 503
BsuRI GGCC 2 cut(s) 289, 411
Cac8I GCNNGC 1 cut(s) 254
CfoI GCGC 1 cut(s) 720
CseI GACGC 1 cut(s) 153
Csp6I GTAC 1 cut(s) 376
CspCI CAANNNNNGTGG 2 cut(s) 375, 410
CviAII CATG 1 cut(s) 569
CviQI GTAC 1 cut(s) 376
DdeI CTNAG 2 cut(s) 74, 696
DrdI GACNNNNNNGTC 1 cut(s) 656
DseDI GACNNNNNNGTC 1 cut(s) 656
EaeI YGGCCR 2 cut(s) 287, 409
Eco24I GRGCYC 3 cut(s) 91, 183, 458
EcoRII CCWGG 1 cut(s) 242
EcoT38I GRGCYC 3 cut(s) 91, 183, 458
FaeI CATG 1 cut(s) 572
FaiI YATR 3 cut(s) 348, 365, 570
FaqI GGGAC 2 cut(s) 564, 570
FatI CATG 1 cut(s) 568
Fnu4HI GCNGC 3 cut(s) 47, 492, 495
FriOI GRGCYC 3 cut(s) 91, 183, 458
Fsp4HI GCNGC 3 cut(s) 47, 492, 495
FspBI CTAG 1 cut(s) 519
GlaI GCGC 1 cut(s) 719
GluI GCNGC 3 cut(s) 47, 492, 495
HaeIII GGCC 2 cut(s) 289, 411
HapII CCGG 1 cut(s) 286
HgaI GACGC 1 cut(s) 153
HhaI GCGC 1 cut(s) 720
Hin1II CATG 1 cut(s) 572
Hin6I GCGC 1 cut(s) 718
HinP1I GCGC 1 cut(s) 718
HindIII AAGCTT 1 cut(s) 292
HinfI GANTC 3 cut(s) 525, 565, 657
HpaII CCGG 1 cut(s) 286
Hpy166II GTNNAC 1 cut(s) 397
Hpy188I TCNGA 1 cut(s) 36
Hpy188III TCNNGA 2 cut(s) 309, 671
Hpy8I GTNNAC 1 cut(s) 397
HpyAV CCTTC 4 cut(s) 54, 465, 530, 703
HpyCH4III ACNGT 5 cut(s) 204, 260, 334, 436, 677
HpyCH4IV ACGT 1 cut(s) 390
HpyCH4V TGCA 4 cut(s) 49, 175, 480, 485
HpyF10VI GCNNNNNNNGC 2 cut(s) 349, 491
HpyF3I CTNAG 2 cut(s) 74, 696
HpySE526I ACGT 1 cut(s) 390
Hsp92II CATG 1 cut(s) 572
HspAI GCGC 1 cut(s) 718
LmnI GCTCC 1 cut(s) 94
Lsp1109I GCAGC 2 cut(s) 33, 503
LweI GCATC 2 cut(s) 4, 437
MaeI CTAG 1 cut(s) 519
MaeII ACGT 1 cut(s) 390
MaeIII GTNAC 4 cut(s) 23, 157, 184, 260
MhlI GDGCHC 4 cut(s) 73, 91, 183, 458
MluCI AATT 2 cut(s) 93, 414
MlyI GAGTC 2 cut(s) 519, 666
MmeI TCCRAC 2 cut(s) 59, 484
MseI TTAA 2 cut(s) 606, 645
MslI CAYNNNNRTG 2 cut(s) 463, 573
MspA1I CMGCKG 1 cut(s) 494
MspCI CTTAAG 1 cut(s) 644
MspI CCGG 1 cut(s) 286
MspR9I CCNGG 2 cut(s) 244, 286
MvaI CCWGG 1 cut(s) 244
MwoI GCNNNNNNNGC 2 cut(s) 349, 491
NciI CCSGG 1 cut(s) 286
NlaIII CATG 1 cut(s) 572
NlaIV GGNNCC 1 cut(s) 90
NmuCI GTSAC 2 cut(s) 23, 260
PfeI GAWTC 1 cut(s) 565
PflMI CCANNNNNTGG 1 cut(s) 624
PkrI GCNGC 3 cut(s) 48, 493, 496
PleI GAGTC 2 cut(s) 519, 665
PpsI GAGTC 2 cut(s) 519, 665
Psp6I CCWGG 1 cut(s) 242
PspGI CCWGG 1 cut(s) 242
PspN4I GGNNCC 1 cut(s) 90
RsaI GTAC 1 cut(s) 377
RsaNI GTAC 1 cut(s) 376
RseI CAYNNNNRTG 2 cut(s) 463, 573
SaqAI TTAA 2 cut(s) 606, 645
SatI GCNGC 3 cut(s) 47, 492, 495
SchI GAGTC 2 cut(s) 519, 666
ScrFI CCNGG 2 cut(s) 244, 286
SduI GDGCHC 4 cut(s) 73, 91, 183, 458
SetI ASST 7 cut(s) 41, 296, 393, 476, 513, 534, 541
SfaNI GCATC 2 cut(s) 4, 437
SmiMI CAYNNNNRTG 2 cut(s) 463, 573
SmlI CTYRAG 2 cut(s) 527, 644
SmoI CTYRAG 2 cut(s) 527, 644
Sse9I AATT 2 cut(s) 93, 414
SsiI CCGC 2 cut(s) 494, 613
SspI AATATT 1 cut(s) 108
SspMI CTAG 1 cut(s) 519
StyD4I CCNGG 2 cut(s) 242, 284
TaaI ACNGT 5 cut(s) 204, 260, 334, 436, 677
TaiI ACGT 1 cut(s) 393
TaqI TCGA 1 cut(s) 462
TasI AATT 2 cut(s) 93, 414
TatI WGTACW 1 cut(s) 375
TauI GCSGC 1 cut(s) 497
TfiI GAWTC 1 cut(s) 565
Tru1I TTAA 2 cut(s) 606, 645
Tru9I TTAA 2 cut(s) 606, 645
TseFI GTSAC 2 cut(s) 23, 260
TseI GCWGC 2 cut(s) 46, 491
Tsp45I GTSAC 2 cut(s) 23, 260
TspDTI ATGAA 4 cut(s) 287, 352, 557, 699
TspGWI ACGGA 1 cut(s) 31
Van91I CCANNNNNTGG 1 cut(s) 624
Vha464I CTTAAG 1 cut(s) 644
XcmI CCANNNNNNNNNTGG 1 cut(s) 202
XspI CTAG 1 cut(s) 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.