Rh5BG388500

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0005691
Physical Location & Seq
Reverse (-)
62163858 .. 62167153
3296 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG388500.1

Sequence Viewer

Length: 513 bp
ATGTTTAGTATAAGCTTACCACTAACCATGAGGAGAATTAAATGCCTGACTGTTGGTATTTGCAATTTCTTTCATTTGGTTTCATATCTAATTGGCTTTTTGTTAAACACAAAACTCCAGGAGTGTCCAAGAATGAGTCTTATGAAGCGGTATGTGCTGCGGTTGTTCATATCATTGAAGTACATCACAGCAAACGTCCTTGACAGAAATAATGGCCGGATTGTTGTAACAGCATCTACAATTGAACATTCAGTTAAGGGCTCGCTCGAGTGTGGCCGCTCTTGCAACGCAAAGGCGGCAGCAGTTGTTGGAGAGGTGCTCGCTATGCGACTTAAGGTTGAAGGTCTTGACCAGGGACAGGGACGAGGGATTCATGTCAATGTAAATAATGAAGTTGAGAAGAAGGGTTTTAAGAACCGTACCAAGGTATGGGCTATTGTCAATGCTCTTAAAAACAATGGAGTTAAACTCATTCTTGATGATAATGATGAAAATAGTTCTCAGCCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

170

Amino Acids

18.94

Weight (kDa)

9.74

Isoelectric Point (pI)

27.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 49 - 118 1.6e-07 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 429
AccBSI CCGCTC 1 cut(s) 279
AciI CCGC 4 cut(s) 148, 160, 277, 296
AcoI YGGCCR 2 cut(s) 214, 274
AfaI GTAC 2 cut(s) 182, 421
AfiI CCNNNNNNNGG 3 cut(s) 358, 424, 429
AflII CTTAAG 1 cut(s) 332
AgsI TTSAA 3 cut(s) 178, 245, 341
AjnI CCWGG 2 cut(s) 117, 351
AluBI AGCT 1 cut(s) 15
AluI AGCT 1 cut(s) 15
Alw21I GWGCWC 1 cut(s) 321
Ama87I CYCGRG 1 cut(s) 266
AoxI GGCC 2 cut(s) 214, 274
ApeKI GCWGC 2 cut(s) 157, 299
AvaI CYCGRG 1 cut(s) 266
BanII GRGCYC 1 cut(s) 263
Bbv12I GWGCWC 1 cut(s) 321
BbvI GCAGC 2 cut(s) 144, 311
BciT130I CCWGG 2 cut(s) 119, 353
BfrI CTTAAG 1 cut(s) 332
BisI GCNGC 4 cut(s) 158, 277, 297, 300
BlsI GCNGC 4 cut(s) 159, 278, 298, 301
Bme1390I CCNGG 2 cut(s) 119, 353
BmeT110I CYCGRG 1 cut(s) 266
BmrFI CCNGG 2 cut(s) 119, 353
BmsI GCATC 1 cut(s) 242
BplI GAGNNNNNCTC 4 cut(s) 303, 335, 453, 485
BpmI CTGGAG 1 cut(s) 101
BsaJI CCNNGG 2 cut(s) 352, 423
Bsc4I CCNNNNNNNGG 3 cut(s) 358, 424, 429
BseBI CCWGG 2 cut(s) 119, 353
BseDI CCNNGG 2 cut(s) 352, 423
BseLI CCNNNNNNNGG 3 cut(s) 358, 424, 429
BseRI GAGGAG 1 cut(s) 46
BseXI GCAGC 2 cut(s) 144, 311
BshFI GGCC 2 cut(s) 216, 276
BsiHKAI GWGCWC 1 cut(s) 321
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 1 cut(s) 217
BslFI GGGAC 2 cut(s) 369, 375
BslI CCNNNNNNNGG 3 cut(s) 358, 424, 429
BsmFI GGGAC 2 cut(s) 369, 375
BsnI GGCC 2 cut(s) 216, 276
BsoBI CYCGRG 1 cut(s) 266
Bsp1286I GDGCHC 2 cut(s) 263, 321
BspACI CCGC 4 cut(s) 148, 160, 277, 296
BspANI GGCC 2 cut(s) 216, 276
BspTI CTTAAG 1 cut(s) 332
BsrBI CCGCTC 1 cut(s) 279
BssECI CCNNGG 2 cut(s) 352, 423
BssT1I CCWWGG 1 cut(s) 423
Bst2UI CCWGG 2 cut(s) 119, 353
Bst4CI ACNGT 2 cut(s) 52, 419
BstAFI CTTAAG 1 cut(s) 332
BstC8I GCNNGC 2 cut(s) 263, 321
BstDEI CTNAG 1 cut(s) 501
BstMWI GCNNNNNNNGC 4 cut(s) 154, 282, 296, 325
BstNI CCWGG 2 cut(s) 119, 353
BstSCI CCNGG 2 cut(s) 117, 351
BstV1I GCAGC 2 cut(s) 144, 311
BsuRI GGCC 2 cut(s) 216, 276
Cac8I GCNNGC 2 cut(s) 263, 321
Csp6I GTAC 2 cut(s) 181, 420
CviAII CATG 2 cut(s) 28, 374
CviJI RGCY 7 cut(s) 15, 96, 216, 261, 276, 434, 505
CviKI_1 RGCY 7 cut(s) 15, 96, 216, 261, 276, 434, 505
CviQI GTAC 2 cut(s) 181, 420
DdeI CTNAG 1 cut(s) 501
EaeI YGGCCR 2 cut(s) 214, 274
Eco130I CCWWGG 1 cut(s) 423
Eco24I GRGCYC 1 cut(s) 263
Eco88I CYCGRG 1 cut(s) 266
EcoRII CCWGG 2 cut(s) 117, 351
EcoT14I CCWWGG 1 cut(s) 423
EcoT38I GRGCYC 1 cut(s) 263
ErhI CCWWGG 1 cut(s) 423
FaeI CATG 2 cut(s) 31, 377
FaiI YATR 9 cut(s) 11, 29, 85, 143, 153, 170, 326, 375, 430
FaqI GGGAC 2 cut(s) 369, 375
FatI CATG 2 cut(s) 27, 373
Fnu4HI GCNGC 4 cut(s) 158, 277, 297, 300
FriOI GRGCYC 1 cut(s) 263
Fsp4HI GCNGC 4 cut(s) 158, 277, 297, 300
GluI GCNGC 4 cut(s) 158, 277, 297, 300
GsuI CTGGAG 1 cut(s) 101
HaeIII GGCC 2 cut(s) 216, 276
HapII CCGG 1 cut(s) 217
Hin1II CATG 2 cut(s) 31, 377
HindIII AAGCTT 1 cut(s) 13
HinfI GANTC 2 cut(s) 136, 370
HpaII CCGG 1 cut(s) 217
Hpy188III TCNNGA 2 cut(s) 347, 476
HpyAV CCTTC 2 cut(s) 335, 397
HpyCH4III ACNGT 2 cut(s) 52, 419
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 2 cut(s) 63, 285
HpyF10VI GCNNNNNNNGC 4 cut(s) 154, 282, 296, 325
HpyF3I CTNAG 1 cut(s) 501
HpySE526I ACGT 1 cut(s) 195
Hsp92II CATG 2 cut(s) 31, 377
LpnPI CCDG 7 cut(s) 59, 104, 131, 230, 338, 344, 365
Lsp1109I GCAGC 2 cut(s) 144, 311
LweI GCATC 1 cut(s) 242
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 1 cut(s) 226
MbiI CCGCTC 1 cut(s) 279
MboII GAAGA 1 cut(s) 412
MfeI CAATTG 1 cut(s) 240
MhlI GDGCHC 2 cut(s) 263, 321
MluCI AATT 4 cut(s) 36, 64, 90, 240
MlyI GAGTC 1 cut(s) 145
MmeI TCCRAC 1 cut(s) 289
MnlI CCTC 3 cut(s) 24, 307, 359
MseI TTAA 8 cut(s) 39, 104, 255, 333, 411, 450, 465, 511
MslI CAYNNNNRTG 1 cut(s) 378
MspCI CTTAAG 1 cut(s) 332
MspI CCGG 1 cut(s) 217
MspR9I CCNGG 2 cut(s) 119, 353
MunI CAATTG 1 cut(s) 240
MvaI CCWGG 2 cut(s) 119, 353
MwoI GCNNNNNNNGC 4 cut(s) 154, 282, 296, 325
NlaIII CATG 2 cut(s) 31, 377
PaeR7I CTCGAG 1 cut(s) 266
PfeI GAWTC 1 cut(s) 370
PflMI CCANNNNNTGG 1 cut(s) 429
PfoI TCCNGGA 1 cut(s) 117
PkrI GCNGC 4 cut(s) 159, 278, 298, 301
PleI GAGTC 1 cut(s) 144
PpsI GAGTC 1 cut(s) 144
Psp6I CCWGG 2 cut(s) 117, 351
PspGI CCWGG 2 cut(s) 117, 351
PspXI VCTCGAGB 1 cut(s) 266
RsaI GTAC 2 cut(s) 182, 421
RsaNI GTAC 2 cut(s) 181, 420
RseI CAYNNNNRTG 1 cut(s) 378
SaqAI TTAA 8 cut(s) 39, 104, 255, 333, 411, 450, 465, 511
SatI GCNGC 4 cut(s) 158, 277, 297, 300
SchI GAGTC 1 cut(s) 145
ScrFI CCNGG 2 cut(s) 119, 353
SduI GDGCHC 2 cut(s) 263, 321
SetI ASST 6 cut(s) 17, 198, 318, 339, 346, 429
SfaNI GCATC 1 cut(s) 242
Sfr274I CTCGAG 1 cut(s) 266
SlaI CTCGAG 1 cut(s) 266
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 2 cut(s) 266, 332
SmoI CTYRAG 2 cut(s) 266, 332
Sse9I AATT 4 cut(s) 36, 64, 90, 240
SsiI CCGC 4 cut(s) 148, 160, 277, 296
StyD4I CCNGG 2 cut(s) 117, 351
StyI CCWWGG 1 cut(s) 423
TaaI ACNGT 2 cut(s) 52, 419
TaiI ACGT 1 cut(s) 198
TaqI TCGA 1 cut(s) 267
TasI AATT 4 cut(s) 36, 64, 90, 240
TatI WGTACW 1 cut(s) 180
TauI GCSGC 2 cut(s) 279, 299
TfiI GAWTC 1 cut(s) 370
Tru1I TTAA 8 cut(s) 39, 104, 255, 333, 411, 450, 465, 511
Tru9I TTAA 8 cut(s) 39, 104, 255, 333, 411, 450, 465, 511
TseI GCWGC 2 cut(s) 157, 299
TspDTI ATGAA 7 cut(s) 62, 72, 157, 158, 362, 405, 504
Van91I CCANNNNNTGG 1 cut(s) 429
Vha464I CTTAAG 1 cut(s) 332
XhoI CTCGAG 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.